• 专利附录
  • 专利说明
  • 权利要求
  • 类似技术
  • 同族专利
  • 引用文献
  • 法律状态
  • 优先权
    • 专利摘要:

    公开号:SU959613A3
    申请号:SU802997519
    申请日:1980-10-24
    公开日:1982-09-15
    发明作者:Гиган Жан
    申请人:Guigan Jean；
    IPC主号:
    专利说明:

    (5Y DEVICE FOR CONDUCTING ANALYSIS
     one-. „-.
    The invention relates to devices for analyzing substances, for example biological, photocolorimetric method.
    A device for carrying out s analysis is known, comprising a rotor with a plurality of peripheral cells distant from its axis with a port for introducing reagents and radial channels connecting the cells with the to device for dosing a liquid, and an analyzer D J
    The disadvantage of the known analyzer is the duration of the analysis due to the time spent on its preparation.
    The purpose of the invention is to accelerate the analysis with a minimum of manipulation.
    The goal is achieved by the fact that a known device for analysis, comprising a rotor with a plurality of peripheral equidistant from its axis cells with a pipe for introducing reagents and radial channels connecting the cells to the liquid dosing device, and the analyzer, is equipped with a means for washing the cells, in each of which there is a peripheral hole d / 1I liquid release and a solid carrier is placed.
    The washing means is made in the form of a central nozzle placed along the axis of rotation of the rotor and connected to the cells by means of radial channels.
    The rotor is made in the form of two placed, one above the other, on contacting surfaces of which there are troughs, radial and peripheral cavities, peripheral edges, which form, respectively, radial channels, cells and peripheral holes when applied.
    Solid carrier formed of one ball.
    权利要求:
    Claims (3)
    [1]
    A solid support is formed by a plurality of balls with a diameter of 10-20 μm, and each cell is provided with a barrier, 39 reinforced Against the peripheral opening. The barrier is a molecular filter. In order to allow the solid carrier to be placed while centrifuging at the level of the peripheral hole, the bottom of each cell is inclined to the peripheral hole and the cell has a volume sufficient to accommodate a solid carrier with the reagent. The cell is made with parallel side walls, of which one is peripheral - equipped with a drainage chute. The radial channels are at the same level as the peripheral holes for the release of fluid. FIG. 1 shows a part of the device, a general view; in fig. 2 - the same, longitudinal section; in fig. 3 is the same, top view; on .fig. k, 5 and 6 cells, where one bead is used as a solid carrier; in fig. 7 and 8 the same (as a solid carrier used a lot of balls); in fig. 9 and 10 is one of the possible variants of cell implementation. The device includes a rotor 1 with. a plurality of peripheral cells 2, each of which contains a capturing solid carrier. 3 made of polystyrene, coated with protein, having the properties of antibodies, a means for washing the cells, made in the form of a central nozzle C, placed along the axis of the rotor’s thrust 5 and connected to the cells 2 by means of radial channels 6 (the above implementation of the means for washing the cells not is the only one: an annular groove may be provided with the joint pipe, radial channels may be formed with a flat collar made on one part of the rotor, a groove on the other, or grooves on both parts of the rotor). Each cell has a peripheral hole 7, an inlet hole 8 and a bottom 9, inclined to the peripheral hole to accommodate a solid carrier by centrifuging at the level of the peripheral hole 7. The rotor 1 can be made in the form of two disks placed one above the other 10 and 11, contact surfaces of which there are gutters 12, radial and peripheral cavities 13, peripheral edges, which, when applied by 34 research institutes, respectively, form radial channels 6, cells 2 and peripheral holes 7. Reagents are supplied to cells 2 when the help of the dropper 15 when turning the rotor 1 at the appropriate angle. The cell volume must be sufficient for placement. ball and reagent without reaching the level of the reagent channel 6. The rotor is rotated by means of drive means 16. Distribution cone 17 is placed in nozzle k for better separation of the flow of feed fluid. In addition, another variant is possible: making a solid carrier in the form of a set of small balls 18 with a diameter of 10-20 µm, with a barrier 19, which is a molecular filter, installed in the cell. The cells can also be made with parallel side walls 20 and 21, one of which is peripheral provided with a drainage chute 22, and the solid carrier is formed by a wall 21 located against the peripheral and covered with protein. The device works as follows. The rotor is positioned under the feeding device so as to inject into each cell 2 through the opening 8 some volume of the first reagent, such as serum, plasma or any other biological fluid containing the substance being analyzed, which is captured by a solid carrier. After injection, a break is taken during the incubation period. The rotor is then rotated and a washing fluid is injected through the central nozzle. The passage through the distribution cone 17, the flushing fluid enters the radial channels 6 and then into the cells. By centrifuging through the peripheral holes 7, excess reagent and washing liquid are removed. By stopping the rotor, it is possible to conduct a simultaneous analysis, a direct analysis using a counter, if radioactive isotopes are used, or indirect, if additional coloring reagent and a reaction stop is used. These operations are then resumed with the second reagent. The proposed device has a simplicity, increased reliability, allows you to quickly and easily receive several dosages necessary for analysis, for example, the thyroid glands. 1. An apparatus for carrying out an analysis, containing a rotor with a plurality of peripheral equidistant cells from its axis. and radial channels connecting the cells with a device for dispensing a liquid and an analyzer, characterized in that, in order to speed up the analysis with a minimum of manipulation, it is equipped with a unit for washing the cells, in each of which a peripheral opening for discharging a liquid is made and a solid carrier is placed,
    [2]
    2. The device according to claim 1, wherein the means for washing the cells is designed as a central nozzle placed along the axis of rotation of the rotor and connected to the cells through radial channels.
    [3]
    3. The device according to claim 1, T.L. and that the rotor is made in the form of two disks placed one above the other, on the contacting surfaces of which there are troughs, radial and peripheral cavities, peripheral edges that form upon imposing respectively radial channels , Cells and peripheral openings. The device according to claim 1, of which is solid, consisting of a single ball. 3 5. Device on PP. I-, characterized in that the solid support is formed by a plurality of beads with a diameter of 10-20 µm, and each cell is provided with a barrier fixed against the peripheral orifice. 6. The device according to PP, 1-5, about the fact that the barrier is a molecular filter. 7. The device according to paragraphs. 1-6, in order to ensure that the solid carrier can be placed during centrifugation at the level of the peripheral hole, the bottom of each cell is inclined to the peripheral hole and the cell has a volume sufficient to accommodate a solid carrier with the reagent. 8. Device on PP. 1-7, that is, the cell is made with parallel side walls, one of which is peripherally provided with a drainage chute. 9. The device according to paragraphs. 1–8 is that the radial channels are at the same level as the peripheral holes for the release of fluid. ri p and o p and t m paragraph: 26.10.79 pp one- . P.01.80 p.n. 5-7 23.05.80p.p. 8.9 Sources of information taken into account in the examination 1. US Patent No. 3953172, cl. 23-230, 27.0.76.
    eight
    1
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    Fig. 9
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    IE50394B1|1986-04-16|
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    IL61287A|1984-08-31|
    AU534275B2|1984-01-12|
    IL61287D0|1980-12-31|
    IN154925B|1984-12-22|
    US4314968A|1982-02-09|
    DK156405C|1990-01-08|
    IE802210L|1981-04-26|
    DK156405B|1989-08-14|
    AR221190A1|1980-12-30|
    MX148876A|1983-06-28|
    引用文献:
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    CH588700A5|1975-02-28|1977-06-15|Hoffmann La Roche|
    US4053284A|1976-03-10|1977-10-11|Akzona Incorporated|Continuous flow apparatus for biological testing|
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    DE3711177A1|1987-04-02|1988-10-13|Dornier System Gmbh|METHOD AND DEVICE FOR OPERATING FLUIDIZED LAYER REACTORS|
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    法律状态:
    优先权:
    申请号 | 申请日 | 专利标题
    FR7926615A|FR2468909B1|1979-10-26|1979-10-26|
    FR8000583A|FR2473723B2|1980-01-11|1980-01-11|
    FR8011511A|FR2483078B1|1980-05-23|1980-05-23|
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