• 专利附录
  • 专利说明
  • 权利要求
  • 类似技术
  • 同族专利
  • 引用文献
  • 法律状态
  • 优先权
    • 专利摘要:
    Apparatus and a method of separating a particulate portion as defined herein from a mixture including a liquid portion, including introducing the mixture into a stream of wash solution flowing along an open bore of a conduit, the wash solution having immiscible fluid segments therein, and subsequent to a separating step, collecting at least one of the aforesaid portions. Combined with these steps is the step of permeating over a period of time the internal surface of the aforementioned conduit with at least the aforementioned liquid portion. This retards the flow of the liquid portion which progressively lags behind the particulate portion until the two portions are separated by the segmented wash solution. Either or both portions may be collected, and when collected, the particulates are in washed condition. The aforementioned separation may be achieved on-line in a sample analyzer, and either or both collected portions may be reacted on-line with a reactant, and at least one product of the reaction may be analyzed on-line.
    公开号:SU784742A3
    申请号:SU772466679
    申请日:1977-03-30
    公开日:1980-11-30
    发明作者:Р. Снайдер Ллойд；Дж. Оберхардт Брюс；Олич Джек
    申请人:Текникон Инструментс Корпорейшн (Фирма)；
    IPC主号:
    专利说明:

    . The invention relates to the separation of mixtures of substances in accordance with the size of their particles, for example, to separation from a mixture of particles with a smaller size, in a liquid state, from particles with large sizes, which can exist in the form of particulates.
    A known method of isolating macro. particles from the mixture, which consists in the formation of the mixture in the form of a stream and its separation on a porous surface. two products with the subsequent analysis of one of them £ 13
    The method is carried out in a device containing a housing made in the form of a pipe with a porous inner surface, inlet and outlet valves.
    The disadvantage of using this method and device in the separation processes is the low efficiency of the separation of particulates from the mixture.
    A known method and device for 25 separation of emulsions £ 2 ^ · The method consists in the fact that the liquid is formed in the form of a stream flowing through an annular channel, divided on a porous surface into two products with <prple3
    In FIG. 1 shows a device, a general view of ', in FIG. 2 - section of core, pus.
    The device consists of a housing 1, a pipe 2 for introducing additional liquid, a pipe 3 for introducing a liquid mixture, a pipe 4 for introducing an immiscible agent. Part, the housing is made in the form of a spiral, 5 and part in the form of a straight segment. The housing consists of an outer tube b and an inner 7, between which an annular channel 8 is formed. The inner tube with an inner channel 9 is made of porous material and can be relatively thin to 0.038 mm. The dimensions of the housing can vary over a fairly wide range, depending on the intended type of mixture to be separated. The outer tube of the housing may be made of suitable plastic, such as nylon.
    The device for outputting the selected fractions consists of a tube 10., which is made of a transparent material, a valve 11 and discharge pipes 12 and 13. On this device, a unit for detecting the selected parts and controlling the valves, consisting of a light source 14, a photodetector 15, is connected. A photodetector is connected with valve conventional logic circuit.
    Carry out the method in this device as follows.
    A washing solution, such as saline solution or water, is continuously injected through the inlet pipe 2 continuously for injection into the pipes and 5. A liquid mixture is introduced into the pipe b through the pipe 3 and the catalytic pipe 4 is cut off segments IQ .35 howl a fixed immiscible agent is cut side by side at certain time intervals to interrupt the flow of solution in the tube b. The liquid mixture can be isolated from similar portions of the wash solution during the introduction of the mixture using portions of an immiscible agent.
    . The segmented stream flowing in tube b enters a long mixing spiral 5, where, due to the presence of portions of an immiscible agent in such a stream, the contents of each liquid segment containing macroparticles are thoroughly mixed so that a significant volume of the liquid part of such a segment contacts repeatedly with the internal flow in channel 9 through a porous surface. The particles are retained, while the fluid in these segments freely passes through the pores of the surface. The flow in channel 9 is not segmented, as a result of which it lags behind the annular flow 8.
    Upon completion of the separation of the liquid of the initial mixture from the macroparticle, the effluent from the tube 5 flows through the tube 10, allowing visual incubation. The analysis of the flowing flow with the particles is carried out portionwise using a light source, 14 and a recording photodetector, which transmits; its signal to the valve 11 activated by the solenoid for its activation in order to distribute the flow and the corresponding outlet pipes 12 and 13.
    The invention can be used to isolate more than two components from a mixture, as well as separating soluble components.
    Example. Blood cells whole blood samples are fixed using a fixative consisting of a 7.4% solution of formaldehyde in sodium phosphate buffer solution (pH-6.7), and the excess fixative is separated from the fixed cells. A specific example relates to this embodiment of the device, which tube 5 is designed so that it has an open inner wall of etched nylon, and the device has a mixing spiral, not shown in the figures and located between the spiral 5 and tube b, the mixing spiral being controlled by temperature. In the nozzle 2 of the tube 6 is continuously supplied with a salt wash solution. Immiscible gas segments are continuously introduced through the nozzle 3 into the saline solution. Through a pipe 4 in combination with a 0.006 megapixel fixative, a sample of whole blood with a volume of 0.007 ml is introduced, which is one of the samples of the sequence intended for supply through the said pipe.
    The tube 5 has a length of 1.32 mm and an effective inner diameter of 1.0 mm. Before entering each sample with a fixative through the pipe 4 and after it through the pipe 2 serves 0,037 ml of saline. Each combined sample and fixative are mixed in a mixing spiral located between tubes b and 5, and the cell part of each sample is fixed during incubation in a mixing spiral. After that, the cell part of the sample is separated from the excess fixative in the tube 5, and the profiled cells are taken into the pipe 13 using the valve 11, and after unloading from the pipe 13 is collected for cytochemical analysis. The performance of the device is 120 samples per hour.
    权利要求:
    Claims (2)
    [1]
    (54) METHOD OF THE INVENTION The invention relates to the separation of mixtures of substances in accordance with the size of their particles, for example, separation from a mixture of smaller particles in a liquid state from particles with larger sizes that may exist in the form of particulates. A known method for isolating particulates from a mixture consists in forming the mixture as a stream and separating it on a porous surface. 15V of product with the subsequent analysis of one of them 1. The method is carried out in a device comprising a housing made in the form of a pipe with a porous inner surface, inlet and outlet valves. The disadvantage of using such a method and device in the separation process is the efficiency of the separation of particulates from the mixture. The known method and apparatus for separating emulsions 2j. The method consists in that the liquid is formed in the form of a stream flowing through the annular channel, divided into a porous surface into two products of a C.P.M. A device for carrying out the method includes a housing made in the form of coaxially arranged pipes, wherein the inner pipe is porous, the pipe of the liquid mixture and the device with valves for outputting the separated fractions. The disadvantage of this process is its complexity, since the seeding is carried out with the imposition of centrifugal forces, as well as insufficient / its effectiveness. The purpose of the invention is to simplify the process and increase the accuracy of the section | No liquid mixture. This goal is achieved by introducing the liquid mixture into the pre-formed stream of additional liquid and, before dividing it on a porous surface, the total stream is divided into parts by portions of unmixed cacti. with him an agent. The device implementing the method is equipped with nozzles for introducing an immiscible agent and additional liquid, and a device for withdrawing implanted fractions with a unit for detecting one of the selected Parts and controlling the valves. FIG. 1 shows the device a general view; in fig. 2 - body section. The device consists of a housing 1, a nozzle 2 for introducing additional liquid, a nozzle 3 for introducing a liquid mixture, a nozzle 4 for introducing an immiscible agent. The part, the body is made in the form of a spiral 5 and the part is in the form of a straight section. The body consists of an outer tube b and an inner 7, between which an annular channel 8 is formed. The inner tube with an inner channel 9 is made of porous material and can be relatively thin to 0.038 mm. The dimensions of the shell may vary over a rather wide range depending on the type of mixture to be separated. The outer tube of the housing may be made of a suitable plastic, such as nylon. The device for outputting the separated fractions consists of a tube 10 ,, the coring device is made of a transparent material, a valve 11 and discharge pipes 12 and 13. On this device there is a unit for detecting the selected parts and controlling the valves, consisting of a light source 14, a photodetector 15. A photodetector is connected to the valve by a conventional logic circuit. Carry out the method in this device as follows. The washing solution, for example, salt solution or water, is continuously injected in the usual way through the introductory tube 2 to provide a flow to the TE) slabs b and 5. A liquid mixture is introduced into the tube b through the nozzle 3 and through the nozzle 4 cut-off segmented unmixing agent at certain time intervals to interrupt the flow of the solution in the tube. The liquid mixture can be isolated from similar portions of the washing solution while the mixture is injected with portions of an immiscible agent. The segmented stream flowing in tube b enters the long mixing coil 5, where, due to the presence of an immiscible agent in such a flow, kg kg of each fluid segment containing macro particles is thoroughly mixed so that a significant volume of the liquid part of such a segment is const repeatedly with an internal flow in channel 9 through the porous PS surface. The particulates are retained, while the fluid in these segments freely passes through the pores of the surface. The flow in channel 9 is not segmented, which is why it lags behind the annular flow 8. Upon completion of the separation of the initial mixture liquid from the macroparticle substance, the flow from the tube 5 flows through the tube 10, which provides visual visibility. The analysis of the flowing stream with particulates is carried out in batches using a light source, 14 and a recording photo detector, which transmits; its signal to a valve 11 triggered by a solenoid for its operation in order to distribute the flow and the corresponding outlet connections 12 and 13. The invention can be used to separate more than two components from the mixture, as well as to separate soluble components. Example. Blood cells, whole blood samples are fixed with a fixative consisting of a 7.4% formaldehyde solution in sodium phosphate buffer solution (pH-b, 7), and an excess of fixative is separated from the fixed cells. A specific example relates to such an embodiment in which the tube 5 is made so that it has an open: the inner wall of the etched nylon, and the device has a mixing spiral, not shown in the figures, and located between the spiral 5 and the tube b, and the mixing spiral is controlled by temperature Ur. In the nozzle 2 of the tube b, salt wash solution is continuously fed. Through tube 3, immiscible gas segments are continuously introduced into the brine. Through the nozzle 4 in combination with 0.006 ml of fixative, a sample of whole blood of 0.007 ml is injected, which is one of the samples of the sequence intended for delivery through the said nozzle. The tube 5 has a length of 1.32 mm and an effective internal diameter of 1.0 mm. Before entering each specimen with fixative through pipe 4 and after it, 0.037 ml of saline solution is fed through pipe 2. Each combi sample and fixative are mixed in a mixing device located between tubes b and 5, and the cell portion of each sample is fixed during incubation in a mixing helix. Thereafter, the cell portion of the sample is separated from the excess fixative in tube 5, and the fixed cells are retracted into tube 13 using valve 11, and after being unloaded from tube 13, they are harvested for cytochemical analysis. The capacity of the device is 120 specimens per hour at ET. Claims 1. A method for isolating particulates from a liquid mixture, comprising forming a liquid in the form of a pot flowing through an annular channel
    dividing it on a porous surface into two products with subsequent separate output and analysis, characterized in that, in order to simplify the process and increase the accuracy of separation, the liquid mixture is introduced into a pre-formed stream of additional liquid and before splitting on a porous surface, the total flow is divided on portions of an agent that is immiscible with it.
    [2]
    2. A device for carrying out the method according to claim 1, comprising a housing made in the form of coaxially arranged pipes, wherein the inner porous pipe, the liquid inlet pipe and the device with a cylinder Hat m for outputting the separated fractions, characterized in that it is provided with introduction of immiscible agent and additional liquid, and the device for discharging the separated fractions is equipped with
    unit to detect one of you. | divided parts and control valve.
    Sources of information taken into account in the examination
    1. Kevin Catts Geoffrey W. Тgegear. Solid Phase Radloimmunassay
    in Antibody-coated Tubes, Sciense, 1967, V. 158. p. 1670-1597.
    2. US Patent 3,791,575, CL,233-18, published. 1974 (prototype).
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    同族专利:
    公开号 | 公开日
    FR2348730B1|1982-06-04|
    GB1574502A|1980-09-10|
    IT1072750B|1985-04-10|
    CH615835A5|1980-02-29|
    JPS52130061A|1977-11-01|
    SE423680B|1982-05-24|
    AU501596B2|1979-06-21|
    AU2403977A|1978-10-12|
    CA1086660A|1980-09-30|
    SE7704535L|1977-10-21|
    US4028056A|1977-06-07|
    BE851998A|1977-09-02|
    NL7702255A|1977-10-24|
    FR2348730A1|1977-11-18|
    DE2716880A1|1977-11-03|
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    法律状态:
    优先权:
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    US05/678,778|US4028056A|1976-04-20|1976-04-20|Substance separation technique|
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