• 专利附录
  • 专利说明
  • 权利要求
  • 类似技术
  • 同族专利
  • 引用文献
  • 法律状态
  • 优先权
    • 专利摘要:
    A vertically oriented centrifuge tube is placed in a rotor cavity or other enclosure, and the open end sealed with a tapered plug. A resilient shell-like cap is placed over the plug such that it is wedged between the plug and the tube. A retaining disc, secured to the open end of the enclosure, wedges the plug and cap into the open end of the centrifuge tube. The mouth of the enclosure is flared to accomodate the plug taper. Alternatively, the flare may be provided by an annular insert, for the cavity, whose interior is flared.
    公开号:SU776542A3
    申请号:SU772555354
    申请日:1977-12-16
    公开日:1980-10-30
    发明作者:Эндрю Романаускас Вильям
    申请人:Е.И.Дюпон Де Немур Энд Компани (Фирма)；
    IPC主号:
    专利说明:

    The invention relates to equipment for the separation of substances into fractions under the action of centrifugal forces and can be used in scientific research in the field of biology, bio-5 chemistry, medicine and other industries.
    Closest to the invention is a centrifuge rotor for separating the preparations according to the density gradient, which is a body of revolution with vertically and concentrically arranged along the periphery and having threaded sections in the upper part of the cells, which serve to accommodate elastic. 15 bones with the studied preparation equipped with conical plugs and locking discs with a thread interacting with the thread of the rotor cells [1}.
    The disk, pressing the conical plug 1 {20 seizes the wall of the elastic container ’between the conical plug and the walls of the rotor cell. Due to the elasticity of the container, a relatively strong, leakproof seal is ensured, effective even at high pressures arising from centrifugation.
    However, although these seals are quite satisfactory, as the diameter of the containers increases, leakage of the test drug can sometimes occur, because with large diameter tanks (more than 2.5 mm), as well as with increasing rotor speed, the pressure on the plug increases.
    The aim of the invention is to increase the tightness of containers.
    This goal is achieved by the fact that in the centrifuge rotor, which is a rotational body with vertically and concentrically arranged along the periphery and having threaded 'sections in the upper part, which serve to' place elastic containers with the studied drug, equipped with conical plugs and locking disks with thread, interacting with the thread of the rotor cells, a recess is made in the wall of each cell under the fixing disk, in which an annular gasket is placed with a gap relative to the wall, the surface of which has a shape similar to that of a cell. .
    In addition, between the wall of each container and the conical plug can be placed additional elastic gasket.
    • 776542
    In FIG. 1 shows a centrifuge * rotor rotor, partial section; in FIG. 2, a container seal made in accordance with a preferred embodiment of a transverse section; in FIG. 3 - the same, in accordance with another possible 5 embodiment. .
    The rotor 1 of the described centrifuge is a body of revolution with cells 2 vertically and concentrically located on its periphery, 10 in which containers 3 for the studied preparation are placed, made of any elastic material, including polyallomers, cellulose nitrate, nylon polypropylene, Upper 15 part of each cell 2 of the rotor has a threaded section 4, elastic containers on top are closed with conical plugs 5, which may have shanks 6 to facilitate their increase after centrifugation. Threaded locking discs 7 mesh with the threads of the cells, are fixed above the shanks of 6 conical plugs 5 and press the latter, jamming the thin elastic walls of the containers 3 between the plugs and the walls of the cells 2. The elasticity of the containers provides a liquid-tight seal, which is quite reliable and It allows speeds of 30 rotor rotations up to 65,000 rpm and higher.
    A recess is made in the wall of each tank 3 under the fixing disk 7
    8, in which an annular gasket is placed with a clearance of 35 • walls
    9, the inner surface of which has a shape similar to that of a cell. With this type of sealing, the conical plug 5 is supported by a third core gasket 9, which can move laterally. Such freedom of movement allows the gasket to adapt to various forces arising from centrifugation. 45
    In addition, when the ring gasket is installed before the centrifuge is actuated, the upper edge of the gasket slightly expands, as a result of which the sealing surfaces 50 are kept in close contact even if they are relatively laterally displaced or deformed.
    According to the second option, done. seal, between the wall of each -> 5 'container' with the 3rd conical plug there is an additional elastic strip 10, which creates an interface between the plug 5 and the container wall, while the preparation under study is последней 0 completely covered by plastic. ‘
    In a preferred embodiment, the additional gasket is made of · a material having an elastic degree equal to or higher than the elasticity of both the container 3 and the plug 5. A material suitable for this purpose is low molecular weight polyethylene, which is particularly preferred because it is more soft, more prone to deformation and more resilient than conventional materials used for centrifuge tubes. Any other known plastics which do not react with container contents may also be used, having sufficient tensile strength and the required low elasticity. This additional elasticity allows greater freedom of relative motion of some parts to absorb mechanical forces arising from centrifugation. In this regard, it should be noted that when using containers made of cellulose nitrate, it is desirable to use ordinary silicone grease, since containers made of cellulose nitrate are more fragile than containers made from other materials.
    The taper of the plug 5 must be less than or equal to the taper of the socket 11 of the neck of the cell 2. Strictly speaking, in the preferred embodiment of the invention, the taper angle cA of the plug, which can be defined as the angle formed between the projection of the circle of the plug and the axis of the cell, can vary between 0 ° and an angle less than 30 °. The angle jb of the socket 11 can vary between 1 ° and 30 ° - the angle fb of the socket is defined as the angle between the axis of the cell and the projection on this axis. In a preferred embodiment of the invention, the angle Jb of the socket is about 16 °, then. as the angle of the axis is 2 ° less or about 14 °. The problem with changing the angles is that if the angles become too small, i.e. approaching zero, then the plug is more difficult to remove, while approaching the angles d and | b to 30 ° or more, more axial force is required for. creating the required jamming and ensuring proper compaction.
    The plug 5 may be made of metal, for example aluminum, or more preferably made of acetal resins, for example, Keirin, the commercial name of the product E.Yu.Dupont de Nemours & Company, Wilmington, Delaware. · '
    In general, the seal provided by this invention is based on increased resilience and movement of parts. This increase in elasticity and movement is ensured in one case by an annular gasket capable of moving and stretching, adapting to various forces arising from centrifugation; on the other hand, elasticity is ensured by the presence of an additional elastic gasket, which is mounted under the conical plug and creates an additional advantage, protecting the plug or container contents from the plug itself. This is especially advantageous when the plug is metallic and prevents the partial transfer of metal ions into the contents of the container.
    权利要求:
    Claims (2)
    [1]
    FIG. 1 shows the rotor center rifuga, a partial section; in fig. 2 a container seal, made in accordance with the preferred version of the design, a part of the cross section; in fig. 3 is the same, in accordance with another possible embodiment. The rotor 1 of the described fugty center is a rotating body with vertically and concentrically located on its periphery cells 2, in which containers 3 for the studied preparation are placed, made of any elastic material including polyallomeres, cellulose nitrate, nylon and polypropylene. The top of each cell 2 of the rotor has a threaded section 4, the elastic containers on top are closed with conical plugs 5, which may have shanks, b to facilitate their increase after centrifugation. The threaded locking discs 7 engage with the thread winding of the cells, are fixed above the shanks 6 of the conical plugs 5 and press the latter, wedging the thin elastic strings of the containers 3 between the plugs and the stacks of the cells 2. The elasticity of the containers ensures a liquid-tight seal which is fairly reliable and allows rotor speeds of up to 65,000 rpm and you tue. . In the wall of each tank 3 under the locking disk 7 there is a groove 8, in which an annular gasket 9 is placed with a gap relative to the wall, the inner surface of which has a shape similar to that of a cell. With such a sealing arrangement, the conic plug 5 rests on the ring gasket 9, which can move in the lateral direction. . the displacement allows the gasket to adapt to the various forces arising from the centrifugation. In addition, when an annular gasket is installed, the center of the rifuga is set in motion, the upper edge of the pad slightly expands, with the result that the sealing surfaces are kept in close contact even if they are relatively lateral in movement or deformation. According to the second embodiment of the seal, an additional elastic gasket 10 is placed between the wall of each container 3 and the conical plug 5, creating an interface between the plug 5 and the wall of the container, which is completely covered by plastic in the last test preparation. In a preferred embodiment, the additional pad is made of a material having a degree of elasticity equal to or higher than the elasticity of both the container 3 and the stopper 5. A suitable material for this purpose is low-molecular-weight polyethylene, which is particularly preferable because That it is softer, more susceptible to deformations, and more resilient than ordinary materials used for centrifuge tubes. Any other known non-reactive plastics that have sufficient tensile strength and the required low elasticity can also be used. This additional resilience allows for greater freedom of relative movement of some parts to absorb the mechanical forces generated by centrifuging. In this connection, it should be noted that when using cellulose nitrate containers, it is desirable to use conventional. Silicone grease because cellulose nitrate containers are more fragile than containers made from other materials. The krnusnost stub 5 must be less than or equal to the taper of the socket 11 neck. cells
    [2]
    2. Strictly speaking, in the preferred embodiment of the invention, the angle CA of the plug taper, which can be defined as the angle formed between the projection of the circumference of the plug and the axis of the cell, can vary between 0 ° and an angle less than 30%. The angle Jb of the socket 11 can vary between 1 ° and 30 ° - the angle {b of the socket is defined as the angle between the axis of the cell and the projection on this axis. In a preferred embodiment of the invention, the fb angle of the socket is about 16 °, then. as the angle ot.on 2 ° less or about 14 °. The problem with changing angles is that if the angles become too small, i.e. approach zero, the plug is more difficult to remove, whereas as the angles o and | b approach 30 ° or more, a greater axial force dl is required. creating the required seizure and ensuring proper sealing. The plug 5 can be made of metal, for example, aluminum, or more preferably it is made of acetal resins, for example, Delrin () - the trade name of the product is E.Yu. Dupont de Nemours & Company, Wilmington, Delaware .; In general, the compaction provided by this invention is based on increased elasticity and movement of parts. This increase in elasticity and movement is provided in one case by an annular gasket capable of moving and stretching, adapting to the various forces that occur during centrifugation; On the other hand, the resilience is provided by the presence of an additional elastic gasket that secures with a conical plug and creates an additional advantage by protecting the plug or the contents of the container from the plug itself. This is particularly advantageous when the plug is metallic and prevents partial transfer of metallic ions into the contents of the container. Claim 1. Centrifugal rotor for section-research institutes of preparations with density gradient, which is a body of rotation with vertically and concentrically located along the periphery and having in the upper part threaded sections cells for placing elastic containers with a test preparation equipped with conical plugs and fixing disks threaded, interacting with the rotor cell thread, which is distinguished with the fact that, in order to improve the tightness of the containers, in the wall of each cell under the fixing disk, on the recess, in which with a gap relative to the wall is placed; an annular gasket, the inner surface of which has a shape similar to that of a cell, 2, a rotor according to claim 1, characterized in that an additional elastic gasket is placed between the wall of each container and the conical cap. Sources of information taken into account in the examination of li USSR Patent for Application No. 2382104, cl. B 04 B 5/00, 1976.
    776542
    类似技术:
    公开号 | 公开日 | 专利标题
    SU776542A3|1980-10-30|Centrifuge rotor for density gradient separation of preparates
    US3434615A|1969-03-25|Centrifuge bottle and closure therefor
    US3366320A|1968-01-30|Centrifuge sample holder
    US4664274A|1987-05-12|Blood-sampling tube
    US3951289A|1976-04-20|Safety-closure device
    US3819111A|1974-06-25|Centrifuge rotor cover
    JP4913166B2|2012-04-11|Cap and cap assembly for centrifuge container
    US3720502A|1973-03-13|Centrifuge test tube stopper
    US8105556B2|2012-01-31|Centrifuge adapter and closure
    GB1559344A|1980-01-16|Phase seperation device
    US7270787B2|2007-09-18|Centrifuge sample jar and closure
    US4080175A|1978-03-21|Internally activated sealing centrifuge test tube cap assembly
    DK173621B1|2001-05-07|Gasket-free closure for carbonated beverage container
    US3281000A|1966-10-25|Closure apparatus
    EP0449425A2|1991-10-02|Self-seal centrifuge tube
    EP1021250A1|2000-07-26|Centrifugally loaded self-sealing integral one-piece cap/closure
    US4166573A|1979-09-04|Centrifuge tube enclosure
    US4372483A|1983-02-08|Fluid containment annulus for fixed angle rotors
    US3443711A|1969-05-13|Vacuum-indicating two-part tamper-proof closure and combination
    IE67280B1|1996-03-20|Cavity sealing system for a centrifuge rotor
    IE914479A1|1992-07-01|Centrifuge bottle having a canted neck
    US4222513A|1980-09-16|Centrifuge tube seal
    JP5240835B2|2013-07-17|Feeding container
    GB2050320A|1981-01-07|Closure for container
    SU580818A3|1977-11-15|Closure for stopping vessels
    同族专利:
    公开号 | 公开日
    IE46019B1|1983-01-26|
    FR2374092A1|1978-07-13|
    IE46019L|1978-06-17|
    DE2756225C2|1984-12-20|
    DE2756225A1|1978-06-22|
    FR2374092B1|1982-03-26|
    JPS5644783B2|1981-10-21|
    GB1596396A|1981-08-26|
    CA1074274A|1980-03-25|
    JPS5389076A|1978-08-05|
    US4114803A|1978-09-19|
    引用文献:
    公开号 | 申请日 | 公开日 | 申请人 | 专利标题
    FR2228718B2|1973-05-08|1980-01-04|Ugine Kuhlmann|
    US3938735A|1975-03-13|1976-02-17|Beckman Instruments, Inc.|Capping assembly for thin all centrifuge tubes|
    US3998383A|1975-07-16|1976-12-21|E. I. Du Pont De Nemours And Company|Gradient separation apparatus|US4222513A|1978-12-12|1980-09-16|E. I. Du Pont De Nemours And Company|Centrifuge tube seal|
    EP0032544B1|1980-01-15|1984-07-25|F. HOFFMANN-LA ROCHE & CO. Aktiengesellschaft|Fixed angle rotor for ultra centrifuge|
    GB2118155B|1982-04-15|1985-11-20|Fisons Plc|Centrifuge tube sealing assembly|
    US4537320A|1983-10-27|1985-08-27|Nielsen Steven T|Centrifuge tube having removable crown and swage fitting|
    US4902270A|1988-10-03|1990-02-20|Nalge Company|Centrifuge tube|
    US4944721A|1988-11-09|1990-07-31|E. I. Du Pont De Nemours And Company|Cavity sealing system for a centrifuge rotor|
    US5382220A|1989-11-07|1995-01-17|E. I. Du Pont De Nemours And Company|Centrifuge tube adapter|
    US5127895A|1990-03-30|1992-07-07|Beckman Instruments, Inc.|Self-seal centrifuge tube|
    DE4233296C1|1992-10-02|1994-03-31|Heitland Und Petre Int Gmbh|Containers for cosmetic products|
    AT253984T|1997-02-14|2003-11-15|Dendreon Corp|METHOD AND DEVICE FOR WASHING CELLS|
    US6080366A|1998-03-02|2000-06-27|Becton, Dickinson And Company|Disposable blood tube holder|
    DE102004062232B4|2004-12-23|2013-01-10|Thermo Electron Led Gmbh|Rotor for laboratory centrifuges|
    JP4761254B2|2005-06-09|2011-08-31|日立工機株式会社|Sample container with sealing mechanism for centrifuge and centrifuge using the same|
    US9913490B2|2013-12-26|2018-03-13|Altria Client Services Llc|Thin wall product display tube|
    CA3043494A1|2016-11-10|2018-05-17|Medisca Pharmaceutique, Inc.|Pharmaceutical compounding methods and systems|
    法律状态:
    优先权:
    申请号 | 申请日 | 专利标题
    US05/751,382|US4114803A|1976-12-17|1976-12-17|Centrifuge tube enclosure|
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