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    • 专利摘要:
    Compounds of the formula <IMAGE> +TR <IMAGE> and pharmaceutically acceptable non-toxic acid addition salts thereof, in which L and D, when applicable, define the chirality; R1 is hydrogen, C1-C3 primary alkyl, or allyl; R2 is hydrogen or C1-C3 primary alkyl, subject to the limitation that when R1 is allyl, R2 is hydrogen; R3 is hydrogen or C1-C3 primary alkyl; R4 is C1-C4 primary or secondary alkyl; R5 is hydrogen or C1-C4 primary or secondary alkyl; R6 is hydrogen or C1-C3 primary alkyl; R7 is hydrogen or C1-C3 primary alkyl; Y is hydrogen or acetyl; Z is hydrogen of <IMAGE> in which R8 is C1-C3 alkyl or hydrogen; and W is isopropyl, -VR9, or -CH2-X-CH3, in which V is O or S, R9 is C1-C4 alkyl or aralkyl, and X is O, S, or -CH2-, subject to the limitation that, when W is isopropyl, R7 is C1-C3 primary alkyl; are useful analgesic agents.
    公开号:SU753358A3
    申请号:SU772526421
    申请日:1977-09-26
    公开日:1980-07-30
    发明作者:Ли Смитвик(Младший) Эдвард；Теодор Шуман Роберт；Куртис Артур Фредериксон Роберт
    申请人:Эли Лилли Энд Компани (Фирма)；
    IPC主号:
    专利说明:

    one
    The invention relates to a method for producing peptides or their salts of compounds having biological activity that can be used in medicine.
    In peptide chemistry, the method for producing peptides by de-blocking the corresponding protected peptides in an anhydrous acidic medium is widely used.
    The purpose of the invention is to obtain new peptides with pharmacological properties using known methods of peptide chemistry.
    The goal is achieved by the described method of producing peptides of general formula I
    where R R R is hydrogen)
    R C ;, -C4 is alkyl;
    R-R is independently hydrogen or methyl,
    -wr -cHjSCH3, -scHjCHj, -sn (sns) g,
     - with
    21 II o
    OR their salts, the fact that OQ that in the compound of formula I
    o e o r i and
    CH — C — K — CH — —HH — CH — C—: lr — CR — —i — .H,
    ““ T R k ia,. C where l is N, “i-n) rm-butyloxycarbyl; R-R, Z and W are as defined above, the protecting groups are cleaved in an anhydrous acidic medium. The process is preferably carried out in trifluoroacetic or glacial acetic acid with gaseous hydrogen chloride or 98% formic acid. Example 1. Preparation of chlorine hydrate of 1-tyrosyl-0-alanylglycyl-1-phenylalanyl-hP-L-methionylamide. A. Benzyl-O-alinat-p-toluenesulf. Nat. To a mixture of 100 ml of benzyl alcohol and 200 ml of benzene containing 55.1 g (0.29 mol) of mono hydrate of p-lolsulfonic acid, 25 g (0.281 mol) of D-alanine are added. The mixture was heated to reflux and the water was removed with a De Na-Stark apparatus. The mixture was heated for 15 hours and then cooled to room temperature and diluted with ether. The resulting precipitate was collected and recrystallized from methanol and ether. 55.3 g (56%) of the whole compound are obtained, m.p. 112-115 ° C. Found,%: C 58.19; H 6.06; N 3.82. C47H5, N05S (351.42), Credited,%: C 58, 6.02; N 3.99. . B. Benzyl N t-butyloxycarb nyl-0-benzyl-1-tyrosyl-0-alinate. To 200 ml of anhydrous N, N-dimethylformamide, 35.1 g (0.1 mol of a part product) is added. The mixture is cooled with stirring to 0 ° C and 11.2 g (0.1 mol) of bicycloooctane diaza is added. Stirring is continued for another 10 minutes at and 37.1 g (0.1 mol) of N -trwt-butyloxycarbonyl-O-benzyl-L-tyrosine is added, then 13.5 g (0.1 mol) of 1- hydroxybenzotriazole and 20.6 g (0.1 mol) of N, M-dicyclohexylcarbodimide. The resulting mixture is stirred for 3 hours at room temperature, then at room temperature for 24 hours. The mixture is cooled again and the suspension is filtered, the filtrate is concentrated in vacuo. Op dissolved in ethyl acetate and carefully promyveiot sodium bicarbonate solution, water, 0,75 N
    ABOUT
    About JC
    n
    w cold citric acid and water again. The organic layer was separated, dried over magnesium sulfate, filtered, and the filtrate was concentrated in vacuo. The residue is dissolved in hot ethanol. Upon cooling, crystals precipitate from the solution, after recrystallization of which from ethanol, 41.5 g (80%) of pure target compound are obtained; m.p. 121-123s; Found,%: C 68.99; H 6.75; N 5.17. Сз «5бМ2 ° б (520,63) It is calculated,%: С 69.21) Н6.97; N. 5.38. B. (p Butyloxycarbonyl-O-benzyl-1-tyrosyl-0-alanine. To a mixture of 200 ml of tetrahydrofuran and 20 ml of water was added 31.2 g (0.06 mol) of part B. The solution was cooled to 0 ° C and 13.2 ml (1.1 squiv) of 5N sodium hydroxide solution are slowly added to it. The mixture is slowly heated to room temperature with stirring. After 5 hours the mixture is distributed between water and ether. The aqueous layer is separated, cooled and adjusted to pH 2. by adding citric acid. The product is then extracted with ethyl acetate. The extract is washed with water, dried over magnesium sulfate, filtered and diluted with ether. The resulting precipitate is filtered off to give 17.7 g (67%) of the title compound, mp 1601620 s. Found: C 64.73; H 6.70; N "MozoHgOb (442.51). Calculated: C 65.14; H 6.83; N 6.63. G. Benzyl N -mpem-butyloxycarbonyl-0-benzyl-1-tyrosyl-0-alanyl glycinate. Mixture of 70 ml of anhydrous dimethylformamide, 6.74 g (0.02 mol) of the benzyl glycinate salt and p-toluenesulfonic acid is cooled to 0 ° C and g (0.020 mol) of diazabicycloooctane is added. The mixture is stirred for several minutes and 8.84 g (0, -020 mol) of the product of Part B are added to it, followed by 2.7 g (0.020 mol) of 1-hydroxybenzotriazole and 4.12 (0.020 mol) of dicyclohexylcarbodiimide. The reaction mixture is stirred at OS for 2 hours and then at room temperature for 24 hours. The suspension is cooled to, filtered and the filtrate is concentrated in vacuo. The residue is dissolved in ethyl acetate. Washed successively with IN solution of sodium bicarbonate, water, cold 0.75N citric acid and water. The organic phase is separated, dried over magnesium sulphate, filtered and the filtrate is concentrated in vacuo. The residue is crystallized from ethanol to give Yu, 8 g (92%) of pure desired compound; m.p. 145-147C.
    Found,%: C, 67.32; H 6.83; N 6.91.
     (589.69).
    Calculated,%: C, 67.22; H 6.67;
     .
    D. N-tert-butyloxycarbonyl-L-tyrosyl-O-alanylglycine.
    To 60 ml of dimethylformamide was added 10.5 g (0.018 mol) of the product obtained as described in Part D, then 2.5 g of 5% palladium on carbon as a suspension in dimethylformamide. The mixture was purged with nitrogen, hydrogen was introduced in a tube at atmospheric pressure and at room temperature. After 3.5 hours, the supply of hydrogen is stopped and the catalyst is removed by filtration. The filtrate is concentrated in vacuo. By treating the residue with ether, 5.4 g (75%) of the title compound are obtained as an amorphous solid.
    Found,%: C, 70.08; H, 5.82; N 6.16.
    C2, HjgN, 05 (446.65).
    Calculated: C 69,94; H 5.87; N 6.27.
    E. N-tert-Butyloxycarbonyl-M-methyl-1-methionylamide.
    The N-tre1U dicyclohexylamine salt (-butyloxycarbonyl-1-methionine (17.2 g, 0.04 mol) is partitioned between ethyl acetate and cold 0.75 N citric acid. The organic phase is separated, washed with water, dried over magnesium sulfate, filtered and concentrated in vacuo to an oily residue. The residue is dissolved in a mixture of 80 ml of anhydrous tetrahydrofuran and 10 ml of dimethylformamide and 0.5 g of 18-crown-6-ether is added. Potassium hydride suspension (equivalent to 0.12 mol) is added with stirring dropwise to the obtained cold mixture over 30 minutes. Then distilled methyl (2.49 ml of 0.04 mol) and the mixture is stirred for 24 hours at room temperature. The cooled reaction mixture is acidified with 0.75 N citric acid to rP 3 and distributed between water and ether. The ether layer is washed several times with water and extracted with 1N sodium bicarbonate solution. The aqueous extracts are combined, acidified to pH 2 and extracted with ethyl acetate. Extracted powder over magnesium sulfate, filtered
    and evaporated in vacuo. Get 8.4 g of a product having an NMR spectrum corresponding to the desired N-methylated product: d 2.92, N — CHj; tf-2.11, S-CHji tf -1,6, C (CH,) 5.
    The oil (8.4 g, about 0.034 mol) is dissolved in 60 ml of dimethylformamide. The solution is cooled before and 4.69 g (0.035 mol) of hydroxybenzothiaol and 7.0 g (0.034 mol) of dicyclohexylcarbodimide are added. The mixture was stirred for 2 hours at 0 ° C and anhydrous ammonia was bubbled through it for 45 minutes. The reaction mixture was then filtered and the filtrate was concentrated in vacuo. The residue was introduced into a silica gel column (0.076-0.252 mm) measuring 3 x 50 cm. Chloroform mixture (9.75: 0.25) of chloroform and methanol was used for elution. The fractions obtained by chromatography are combined on the basis of the results of analysis by chromatography on a thin layer and are concentrated in vacuo, obtained after double recrystallization (from a mixture of ether and petroleum ether) of the desired product (4.1 g); m.p. 75-78 ° C.
    NMR: tf 2; 80, tf 2, 10, SU 1.48, C (CHj) j-, l5 -29.5 ° (С 0.5, chloroform).
    Found,%: C 50.59; H 8.24; N 10.87,
     (262.37).
    Calculated,%: C 50.36; H 8.45; N 10.68.
    J. M-trepp-Butyloxycarbonyl-b-phenylalanil-M-methyl-1-methionylamide
    Anhydrous hydrogen chloride was bubbled through a mixture of 20 ml of glacial acetic acid, 2 ml of anisole, 2 ml of triethylsilane and 3.6 g (0.0144 mol) of the product obtained under the conditions described in part E, for 30 minutes. The mixture is then diluted with ether. The precipitate formed is filtered off, dried (2.9 g) and redissolved in 40 ml of dimethylformamide. The solution is cooled before and 2.9 ml (0.0146 mol) of dicyclohexylamine and then 1.97 g (0.0146 mol) of hydroxybenzothiazole, 3.87 g (0.0146 mol) of N -mpem-butyloxycarbonyl 1-phenylalanine and 3.0 g (0.0166 mol) of dicyclohexylacarbdimide. The resulting mixture was stirred for 2 hours at 0 ° C, and then 24 i at room temperature. Cool again, filter, concentrate the filtrate in vacuo. The residue is dissolved in ethyl acetate and the solution is washed successively with 1 N sodium bicarbonate solution, water, 0.75 N citric acid, and water. The ethylacetylene solution was dried over magnesium sulfate, evaporated in vacuo to give an oil that did not crystallize from petroleum ether. The residue is introduced into a column (3x50 cm) filled with silica gel and the product is eluted with chloroform, a mixture of chloroform and methanol (9.8: 0.2). A residue is obtained from the fractions combined according to the result of chromatography on a thin layer. which, after recrystallization from a mixture of ether and petroleum ether, gives 3.1 g (52.5%) of the desired compound, m.p. EE-SW C.
    Found,%: C 59.14; H 7.47; N. 10.45,
     (409.55).
    Calculated: with 58.65 N 7.63; N 10.26,
    3, N-mpem-Butyloxycarbonyl-L-tyrosyl-D-apanylglycyl-L-phenyl-alanyl-N-methyl-L-methionyl amide.
    2.2 g of a mixture of 20 ml of glacial acetic acid, 3 ml of anisole and 3 ml of triethylsilane are added.
    (5.37 mol) of the product obtained under the conditions described in part G, anhydrous hydrogen chloride is passed through the mixture for 30 minutes. Ether is then added, the precipitated precipitate is filtered off and dried in vacuo. The solid (1.75 g, 5 mol) is dissolved in 30 ml of anhydrous dimethylformamide and the mixture is cooled to, the neutral hydrochloride salt is added by adding 0.99 ml (5. mol). dicyclohexylamine. After 5 minutes, 2.05 g (5 ml) of the product obtained under the conditions described in part D were introduced, followed by 0.68 g (5 mol), oxybenzothiazole and 1.03 g (5 mol) dicyclohexylcarbodiimide. Mixture stirred for 24 hours at C. The resulting insoluble product is filtered off and the filtrate is evaporated in vacuo. The residue is dissolved in ethyl acetate, the solution is washed successively with IN, an aqueous solution of sodium bicarbonate, cold 0.75 N citric acid and water, the solution is dried over magnesium sulfate, introduced into a column (3x50 cm) with silica gel and the product is eluted with chloroform, and then the mixture chloroform and methanol (9: 1). The fractions are combined, analyzed by thin layer chromatography, evaporated, two batches of raw product weighing 0.80 and 1.2 g are obtained. The first portion is further purified by chromatography on a thin layer of silica gel (chloroform methanol 9 : 1) and get 0,62 g intact Vågå compound as an amorphous solid.
    Found,%: C 58, 48; H 6, 64, N 11.97,
    With “N..MbOe5 (700,86).
    Calculated,%: C 58.27; H 6.90; N 11.99,
    Amino Acid Analysis: Found Dex 0.99i Dea 1.00; sgu 1.00; Phe 1.00,
    The second portion of the product is chromatographed twice by the same method and 0.74 g of the desired product is obtained, which had the elementary analysis and analysis of the amino acid.
    And, 1-tyrosyl-0-alanylglycyl-L-phenyl alanyl-N-methyl-L-methionylamide hydrochloride,
    To 5 ml of glacial acetic acid, containing 0.2 ml of anisole, 0.72 g (1.03 mol) of the desired compound of part 3 is added. Anhydrous hydrogen chloride is passed through the mixture for 20 minutes, the mixture is freeze-dried, to give 0, 74 g of the title compound; Rj 0.3.
    An analytical sample of the product. dried in vacuum at 100
    Found,%: C 54.36; H 6.19; N 13.00.
    , M Ob5SV (637.20).
    Calculated,%: C 54.5b; n 6.49; N 13.19.
    Amino Acid Analysis: Found 1,01; AYA 0.99; Ceu 1.00; Phe 1.00.
    Example 2. Preparation of mono-acetate of 1-tyrosyl-0-leucylglycyl-1-phenylal anyl-M-methyl-b-methionyl amide one-and-a-half hydrochloride monoacetate.
    A. Benzyl-O-leucine-p-tolylsulfone at.
    The compound is prepared under the conditions described in Part A of Example 1 to prepare the 0-alinate derivative. Yield 73%; T..PL. 155-156 S.
    Found,%: C 61.17; H 6.68; N 3.81.
    Ca / liiNs-S (393.50).
    Calculated,%: C 61.05; H 6.92; iN 3.56.
    B. Benzyl-M gpre-butyloxycarbonyl-0-benzyl-1-tyrosyl-0-leucine.
    To 50 ml of dimethyl forms, 7.86 g (0.020 mol) of the product obtained under the conditions described in H part A are added. The mixture is cooled to 0 ° C and 2.24 g (0.020 mol DA8CO is added. The mixture is stirred for 5 minutes and 7, 42 g (0.020 mol) of M-tert-butyloxycarbonyl-O-benzyl-L-tyrosine, and then 2.7 g (0.020 mol of oxybenzothiazole and 4.12 g (0.02 mol of dicyclohexylcarbodiimide) are added. The mixture is stirred 2 h at 0 ° C and 24 h at room temperature. Then the mixture is cooled again to 0 ° C, the resulting suspension is filtered, and the filtrate is concentrated in vacuo.The resulting residue is dissolved in ethyl acetate, p the target is washed successively with 1 N sodium bicarbonate solution, water, cold 0.75 N citric acid and water. The organic phase is dried over magnesium sulfate, filtered and the filtrate is concentrated in vacuo. The residue is crystallized from hot ethnol and 9.0 g are obtained (78% ) the target compound; mp 100-103 ° C.
    Found,%: C 71.30; H 7.15, N 4.79.
    CgiH NjO (574.72)
    Calculated,%: C n 7.37; N 4.87.
    B. N-Yaret-Butyloxycarbonyl-0-benzyl-L-tyrosis yl-0-leucine.
    To 80 ml of tetrahydrofuran was added 8.0 g (0.0193 mol) of the product obtained under the conditions described in part B. After adding 20 ml of water, the mixture was cooled to 0 ° C and 7.25 MP were slowly added to it. (0.0145 mol) 2N aqueous solution of sodium hydroxide. The mixture was stirred for 30 minutes at 0 ° C and 24 hours at room temperature, then partitioned between water and ether. The aqueous phase is separated, cooled to 0 ° C, acidified with 1 N hydrochloric acid to pH 2. The product is extracted with ethyl acetate. The extract is washed with water, dried over magnesium sulfate, filtered and concentrated in vacuo. Get syrupy residue. This residue is crystallized from a mixture of ether and petroleum ether, and 6.4 g (95%) of the desired compound are obtained; m.p. 90-94 0.
    Found,%: C 67.14; H 7.38, N 5.76.
     (484.59),
    Calculated,%: C, 66.92;
    H 7.49; 5.78.
    N
    G. Benzyl-M-tributyloxycarbonyl-O-benzyl-L-tyrosyl-0-leucylglycinate.
    To a mixture of 3.37 g (0.010 mol) of benzyl glycinate salt and p-toluenesulfonic acid and 1.12 g (0.010 mol) of ABCO in 25 ml of anhydrous dimethylformamide were added 4.84 g (0.010 mol) of the compound obtained under the conditions described in Part B. The mixture is cooled to 0 ° C and 1.35 g (0.010 mol) of hydroxybenzothiazole and 2.06 g (0.010 mol) of dicyclohexylcarbodiimide are added to it. The resulting mixture was stirred for 2 hours at Oc and 24 hours at room temperature. After cooling to 0 ° C, the mixture is filtered and the filtrate is concentrated in vacuo. The residue was dissolved in ethyl acetate, and the solution was washed successively with a 1 N aqueous solution of nari bicarbonate, water, cold 0.75 N citric acid, and water. After drying over magnesium sulfate, the solution is filtered and concentrated in vacuo. The residue is crystallized from aqueous ethanol to give 4.0 g (63%) of the desired compound; m.p. 114-116 ° C ..
    Found,%: C 68,17; H 7.12; N -6.40.
     (631.77).
    calculated,%: C 68.44; H 7.18; M f;, 65.
    D. M-y cb -Butyloxycarvinyl-1-tyrosyl-0-leucylglycine.
    3.9 g (0.006 mol) was added to 5 ml of anhydrous dimethylformamide; the compound obtained under the conditions described in part D and 1.5 g of 5% palladium on carbon. After adding 40 ml of ethanol, the mixture is purged with nitrogen and hydrogen is introduced into it at 5 hours at atmospheric pressure and at room temperature. The catcher is filtered and the filtrate is evaporated in vacuo. The residue is crystallized from a mixture of ether and ethyl acetate, to obtain 2.3 g (85%) of the target compound: Ne; m.p. 189-190 s.
    5; Found: C 58.79; H 7.48; N 9.39.
    C. (451.52),
    Calculated,%: C 58.52 H 7.37 N 9.31.
    0
    E. N -mpem-Butyloxycarboyl-L-tyrosyl-0-leucylglycyl-1-phenylalanyl-M-methyl-1-methionylamide. 0.692 g (0.002 mol) is added to 10 ml of anhydrous dimethylformamide.
    5 hydrochloric acid salt of L-phenylalanyl-N-methylmethionylamide (prepared as described in part 3 of example 1) and 0.903 g (0.002 mol) of the product, the preparation of which is described in
    0 parts D. The resulting mixture is cooled to OOS and 0; 28 ml (0.002 mol) of triethylamine and after 10 minutes 0.27 g (0.002 mol) of hydroxybenzothiazole and 0.412 g (0.002 mol) are added
    5 dicyclohexylcarbodiimide. The mixture is stirred for 2 hours at 0 ° C and 24 hours at 4 ° C. The resulting precipitate is filtered off and the filtrate is concentrated in vacuo to a residue, which is dissolved in ethyl acetate. The solution is washed successively with 1 N aqueous sodium bicarbonate solution, water, cold 0.75 N citric acid and water. The organic phase is separated, dried over magnesium sulfate, filtered, and the filtrate is concentrated in vacuo. A chromatography is performed in a thin layer and the product is eluted from the plate with a mixture of chloroform and methanol (9.25: 0.75). From each
    0 plates cut out the main UV-positive band and the product was eluted with silica gel with a mixture of chloroform and methanol. After removal of the solvent in vacuo, 1.2 g (81%) are obtained.
    5 target compound as an amorphous solid, -31.5 ° (C 0.5, methanol) ..
    Found,%: C 59.88, H 7.06; N 11.15.
    0
    Cj Hs NiOgS (742.93).
    calculated%: C 59.88; H 7.06; H 11.15.
    Aialie amino acids: Found Tug 5 Leu 1,00, Gey 1,00; Phe 0.99. g. L-thyroyl-O-leucylglycyl-b-phenylalanyl-K-meth &amp; l-1-m4thionyl amide monoacetate (1.5). To 5 ml of glacial acetic acid containing 0.3 ml of anisole, add 0.900 g (0.0012 mol) of the compound, the preparation of which is described in part. Anhydrous hydrogen hydroxide is passed through the mixture for 20 minutes. The product is dried by lyophilization, the target compound is obtained from acetic acid as an amorphous solid; - 2.1 ° (with 0.3, m tanol). Found,%: C 54.30; H 6.64 N 11.32; every 6.96. CaiH rNfiOfiS- 15 not- CdNdOg (757, Calculated,%: C 53.93; H 6.79; N 11.10-cce 7.02; amino acid analysis: Found Tu 0.99; Leu 1.03; GPy 0.99; Phe 0.9 Example 3. Preparation of tyrosyl-0-alanylglycene-L-phenylalanyl-L-methionylamide hydrochloride 1. A. Methyl-N-thrung-butyloxycarbyl nyl-1-phenylalanyl-1-methionate. To a mixture of 200 ml of dimethylformamide and 19.9 g (0.1 mol) of methyl L-methionate hydrochloride, cooled to 0 ° C, 19.9 ml (0.1 mol) of dicyclohexylamine, 26.5 g (0, 1 mol) yg ret-butyloxycarbonyl-1-phenylalanine, 13.5 g (0.1 mol) of oxybenzothiazole and 20.6 (0.1 mol) of dicylohexylcarbodiimide. The mixture is stirred for 2 hours at room temperature for 24 hours and then cooled again to 0 ° C and the precipitate formed is filtered off. The filtrate is concentrated in vacuo and the residue is dissolved in ethyl acetate. The solution is washed successively with cold 0.75 N citric acid, water, 1 N sodium bicarbonate solution and water. The ethyl acetate layer was dried over magnesium sulfate and evaporated in vacuo to obtain a crystalline residue. The residue is recrystallized twice from a mixture of ether and petroleum ether, and 26.6 (65%) of the title compound are obtained; t. square 8992bc Found,%: C 58.41; H 7.15; N 6.71. CjoHiJ iO S (410,53). Calculated,%: C 58.51; H 7.37; N 6.82. B. N-tert-butyloxycarbonyl-L-phenylapanine-1-methionylamide. To 60 ml of methanol was added 13.0 of the compound obtained under the conditions described in part A. The resulting suspension was placed in a thick-walled bottle equipped with a stirrer. The mixture is cooled to -78 ° C and 60 ml of liquid anhydrous ammonia is added to it. The reaction vessel is closed and the contents are allowed to warm to room temperature, at which the liquid is stirred for 24 hours. Then the vessel is slowly cooled again to -78 ° C and open up. The ammonia residue is evaporated by heating the mixture and the product obtained after evaporation of methanol is recrystallized from methanol, 9.7 g (77%) 192-195 s are obtained. target compound; m.p. Found,%: C 57.41; H 7.17; N 10.37. C gH NgOijS (395.52). Calculated,%: C 57.70; H 7.39; N 10.62. B. 1-phenylalanil-1-methionylamide hydrochloride. To 150 ml of glacial acetic acid containing 10 ml of anisole and 10 ml of triethylsilane were added 9.6 g (0.024 mol) of the product of part B. Then anhydrous hydrogen chloride was passed through the mixture using a gas dispersing tube and after 30 min the reaction the mixture is diluted with ether. The precipitate formed is recrystallized from a mixture of ethanol and ether to obtain 7.5 g (94%) of the desired compound; m.p. 214-216 C. Found;%: C 50.75, H 6.84; N 12.54. C ,, OaSCe (331,87). Calculated,%: C 50.67; H 6.68; N 12.66. G. N -mpeni-Butyloxycarbonyl-O-alanylglycyl-L-phenylNIL-L-methionylamide. 1.66 g (0.005 mol) of the product described in part B and dicyclohexylamine (0.99 ml, 0.005 mol) were added to 4O, ml of dimethylformamide and the solution was stirred while cooling to. Then 0.88 g (0.005 mol) m-grresh-butyloxycarbonylglycine, 0.68 g (0.005 mol) oxybenzothiazole and 1.03 g (0.005 mol) dicyclohexylcarbodiimide are added to the mixture. The resulting mixture was stirred for 2 hours at 0 ° C, and then 24 hours at room temperature. The precipitate formed after re-cooling the mixture before is filtered off and the filtrate is evaporated in vacuo. The residue is dissolved in ethyl acetate and the solution is washed successively with 1 N sodium bicarbonate solution, water, cold 0.75 N citric acid and water. The organic phase is dried over magnesium sulphate, filtered and evaporated in vacuo. The residue is dissolved in hot ethyl acetate. After cooling, a gel forms, which does not undergo crystallization, the gel is filtered, and dried, to obtain 1.7 g of an amorphous solid. The substance is suspended in 50 ml of acetonitrile containing 5 ml of anisole and 5 ml of triethylsilane. After adding p-toluenesulfonic acid monohydrate, the mixture is stirred for 5 hours. The precipitate is filtered off and dried, and 1.6 g (0.003 mol) of crude p-toluenesulfonic acid and 1-phenylalanyl-1-methionylamide glycyl are obtained. This product is dissolved in 30 ml of anhydrous dimethylformamide, cooled to 0 ° C, 0.336 g (0.003 mol) of DABCO was added And after 10 minutes 0.8 g (0.004 mol) of M -gorep -butyloxycarbonyl-O-alanine, 0.540 g (0.004 mol) of oxybenzothiazole u0, 824 g ( 0,004 mol) dicyclohexylcarbodiimide. The resulting mixture was stirred for 2 hours at 48 hours and at room temperature. Again cooled to 0 ° C and filtered, the filtrate is evaporated in vacuum. The residue was dissolved in butanol, and the solution was washed successively with 1 N sodium bicarbonate solution, water, cold 0.75 N citric acid, and water. The organic phase is dried over magnesium sulphate, filtered and concentrated in vacuo. The residue was dissolved in hot ethanol, and with the addition of ethyl acetate, the desired compound was obtained as a precipitate (1.1 g, 42%).
    Amino Acid Analysis: Found 1.01; Giy 1.01; Phe 1.01; Met 0.98.
    D. M -trego-Butyloxycarbonyl-1-tyrosyl -o-alanylglycyl-1-phenylalanyl-L-methionylamide.
    To a mixture of 20 ml of acetic acid, 2 ml of anisole and 2 ml of triethylsilane was added 1.0 g (0.0019 mol) of the product described in Part G. The gas was dispersed into the mixture during 30 min anhydrous hydrogen chloride. Ether is then added to the reaction mixture and the precipitate formed is filtered off and dried. 0.870 g of a solid is obtained, which is dissolved in a mixture of 20 ml of cold (0 ° C) dimethylformamide and 0.38 ml (0.0019 mol) of dicyclohexylamine. After 10 minutes, 0.534 g (0.0019 mol) of N-rt pbm-butyloxycarbonyl-L-tyrosine, 0.257 g (0.0019 mol) of oxybenzotazole and 0.391 g (0.0019 mol) of dicyclohexylcarbodiimide are added to the mixture. Stirring is continued at 2 hours and at room temperature for 24 hours. After cooling the mixture again, the precipitate formed is filtered and the filtrate is concentrated in vacuo. The resulting residue is dissolved in butanol, and the solution is washed successively with 1 N sodium bicarbonate solution, water, cold 0.75 N citric acid, and water. The organic phase is dried over sulfate, magnesium, filtered and the filtrate is concentrated in vacuo. When trying to crystallize the residue from ethyl 1gate or ethanol, a gel is obtained. The residue is dissolved. in hot methanol and the solution is applied to a chromatographic plate
    in a thin layer, a mixture of chloroform and methanol (9: 1) is used for elution. The corresponding band is cut from the plate and extracted with a mixture of chloroform and methanol. After evaporation of the solvent in vacuo, 0.270 g (21%) of the desired compound are obtained; Rj 0.17.
    Amino Acid Analysis: Found Ty; ACa 1.02; cross at 0.99; Phe 1.02; Met 0.98.
    E. L-tyrosyl-O-alanylglycyl-1-phenylalanyl-1-methylionyl amide hydrochloride.
    To 5 ml of glacial acetic acid containing 0.25 ml of anisole, 0.270 g (0.0004 mol) of the product described in part D are added. Hydrogen chloride is introduced into the mixture using a dispersing tube. the mixture is cooled and lyophilized to give 0.182 g (75%) of the desired compound; R 0.5.
    0
    Amino Acid Analysis: Found Tug 0, ACa 1,00; GSy 0.99; Fhe 1.01; Met 0.91.
    This analysis showed the presence of methionine sulfoxide.
    five
    PRI me R 4. Preparation of t-tyrosyl-D-ala nylglycyl-N-methyl-L-aryl-N-methyl-L-methionylamide thydrosyl-hydrochloride, three-aqueous hydrochloride.
    A. N-ropeg (-Butyloxycarbonyl-N 0 -methyl-1-phenylinatate-M, N-dicyclohexylammonium.
    To 80 ml of anhydrous tetrahydrofuran was added 5.3 g (0.02 mol) of M-Clap-butyloxycarbonyl-1-phenyl 5 alanine. The resulting solution is cooled to approximately 10 ° C and 10 ml of anhydrous dimethylformamide and 0.5 g of 18-crown b-ether are added. Then, 10.15 g (containing 0.060 mol KN) of an oily dispersion of potassium hydride are slowly added to the mixture. The resulting mixture is cooled. And 1.24 ml (0.020 mol) of methyl iodide are added to it. Stirring at room temperature
    5 continue for 24 hours. Then the mixture is poured into crushed ice and extracted with ether. The aqueous phase is acidified to pH 2 with citric acid and the product is extracted with ethyl acetate. The organic phase is washed with water, dried over magnesium sulphate and concentrated in vacuo. A noncristaping syrup is obtained, the NMR spectrum of which corresponds to the intended aqueous production.
    NMR ICf 2.72, N-CH; Cfl, 35, C (CHj).
    The syrup is dissolved in ether and 4.0 ml of dicyclohexyl amine is added to the solution. Upon cooling, crystals precipitate out of which, after crystallization from a mixture of methanol-ester, 6.8 g (74%) of the desired compound are obtained; T.SH1. 171-1740s; h: 5 -22.0 ° 5 (C "1, methanol). Found,%: C 70.60J H 9.49; N 6.19. , N40 | (460.66). Calculated,%: C 70.40 H 9.63, N 6.08. B. M-tert-Butyloxycarbonyl-n-methyl-L-phenylalanyl-N-methyl-L-methionyl 1mide. A mixture of 30 MP of anhydrous dimethylformamide containing 1.98 g (0.010 mol) of the M-methyl-1-methionylamide hydrochloride salt and 4.16 (.0.010 mol)) et-butyloxycarbonyl-M-methyl-1-phenylalanine is mixed for 5 minutes, then it is cooled to OC and oxybenzotaeol (1.35 g, 0.010 mol) and dicclohexy carbodiimide (2.06 g, 0.010 mol) are added. This mixture was stirred at-0 ° C for 2 hours and filtered at room temperature for 24 hours. The precipitate that formed was filtered and the filtrate was concentrated in vacuo to give a syrup, which was dissolved in ethyl acetate. This solution is washed successively with a solution of sodium bicarbonate, water, cold 0.75 N citric acid, and water. The organic phase is dried over magnesium sulphate and concentrated in vacuo to syrup. The syrup is dissolved in chloroform, added to a column (3x50 cm) with silica gel and the product is eluted with chloroform, and then a mixture of chloroform with methanol (9.75: 0.25), the fractions are combined and after concentration in vacuo, 1.4 g are obtained (33%) of syrup that had an NMR spectrum corresponding to the spectrum of the target dyneni, NMR: cf 2.93; N-CHgPheV Cf 2.73; N-CH.Met; (f 2.10; s-CH ;; cf 1.37 s (cn) h. c. N — Pfiro-Butyloxycarbonyl-L-tyrosyl-O-alanylglycyl-M-methyl-i-phenyl-schanyl-M-methyl-b -methionyls To a mixture of 5 ml of glacial acetic acid, 1 ml of anisole and 1 ml of triethylslyane, 1.4 g (0.0033 mol) of part B product was added. Anhydrous hydrogen chloride was passed through the mixture for 30 minutes, then the reaction mixture the mixture is diluted with ether. The precipitate formed is isolated and dried (1.1 is then dissolved in 40 ml of dimethylformamide. To the cooled mixture, 1.27 g (0.0031 mol) of product are added, the preparation of which is described in part D of example 0.420 g (0.0031 m or l) hydroxybenzothiazole and 0.640 g (0.0031 mol) of dicyclohexylcarbodiimide. After 10 min, 0.43 g (0.0031 mol) of three ethylamine are added and stirring is continued at 2 h and at 4 ° C - 48 h. the precipitate is filtered off and the filtrate is concentrated under vacuum to a syrup, which is then dissolved in ethyl acetate. The solution is successively washed with 1 N sodium bicarbonate solution, water, cold 0.75 N citric acid and water, then dried over magnesium sulfate, filtered and the filtrate is concentrated in vacuum to obtain 2.0 g of crude product. The product was dissolved in chloroform, dissolved in a column (3x50 cm) with silica gel, and the product was eluted with chloroform and then with a mixture of chloroform and methanol (9: 1). The fractions containing the desired product are combined, concentrated in vacuo to obtain 1.1 g (47%) of the non-crystalline target compound. Found,%: C 59.01; H 6.78; N 11.58. (714.88). Calculated,%: C 68.80; H 7.05; N 11.76. G. L-thyro3-D-alanylglycyl-N -methyl-O-phenyl-alanyl-N -methyl-L-methionyl-amide three-water hydrochloride. To a mixture of 10 ml of glacial acetic acid and 0.5 ml of anisole was added 0.70 g (0.001 mol) of the product described in part B. Anhydrous hydrogen chloride was passed through the mixture for 20 minutes. The reaction mixture is then cooled and lyophilized to obtain 0.678 g of a hygroscopic target compound. Found,%: C 51.13; H 6, 97; N 11.72. CsoH jNeOgSCe (705.23). Calculated,%: C51,08; H7,0; N 11.91 .. Amino Acid Analysis: Found 1.0%; ACa 1.01; Ggy 0.96. Example 5. Preparation of L-tyrosyl-0-alanyl-1-alanyl-1-phenylalanyl-m-methyl-1-methionylamide monoacetate-1,25-hydrochloride monoacetate. A. Benzyl-M-tert-butyloxycarbonyl-0-benzyl-1-tyrosyl-0-alanyl-0-alinate. To a solution of 3.19 g (0.010 mol) of the p-toluenesulfonate salt of benzylinate in 30 ml of anhydrous dimethylformamide was added 4.43 g (0.010 mol) of the product described in Part B of Example 1. The mixture was cooled to 0 ° C and added to 1.12 g (0.010 mol) of DABCO, and then after 10 min - 2.135 g (0.010 mol) of oxybenzotazole and 2.06 g (O, 010 mol) of dicyclohexylcarbodiimide. The resulting mixture was stirred for 2 hours at 48 hours and at room temperature. The precipitate formed is filtered off and the filtrate is evaporated in vacuo to a syrup. The syrup is dissolved in ethyl acetate and the solution is washed successively with IN solution of sodium bicarbonate, water, cold 0.75 N hydrochloric acid and water. The organic phase is dried over magnesium sulphate and filtered. The filtrate is concentrated in vacuo to give a residue which does not crystallize from ethanol or-ether. Upon dilution of the ether solution with petroleum ether, a gel is obtained, which is filtered and dried in vacuo. The crude amorphous product (4.0 g) is introduced into a silica gel column and the product is eluted with chloroform, then with a mixture of chloroform and methanol (9.75: 0.25) . Combining the fractions containing the desired product, the eluate is evaporated in vacuo to give a syrupy residue. This residue is dissolved in ether and, when petroleum ether is added to the solution, 3.0 g (50%) of the title compound is obtained as an amorphous solid, m.p. 100-104 ° C. Found,%: C 67.56; H 6.60; N 7.16. C3jH, iNjOr (603.72). Calculated,%: C 67.64; H 6.85, N 6.96. B. N-mpem-Butyloxycarbonyl-1-tyrosyl-0-alanyl-and-alanyl. 2.9 g (0.0048 mol) of the product described in Part A are added to 5 ml of anhydrous dimethylformamide. Then, 1.0 g of 5% palladium on carbon and 50 ml of ethanol are added to the mixture. Hydrogen was introduced into the mixture for 6 hours at atmospheric pressure and at room temperature. The reaction vessel is then purged with nitrogen, the catalyst is filtered off and the filtrate is concentrated in vacuo. The residue is dissolved in ethyl acetate and the solution is diluted with ether. The precipitate formed is filtered off and dried under vacuum. 1.5 g (74%) of the expected compound are obtained in the form of an amorphous solid; qCj 25.9 °. (C 5, chloroform). Found,%: C 56.80; H 9.95; 9.81, (423.47), Calculated,%: C 56.73; H 6.90; N 9.92. B. N -glret-Butyloxycarbonyl-L-tyrosyl-O-alanyl-L-alanyl-L-phenylalanyl-M-methyl-1-methionylamide. A mixture of 10 ml of anhydrous dimethylformamide, 0.692 g (0.002 mol of the hydrochloride salt of L-phenylalanine-H-methyl-1-methionylamide (prepared as described in part 3 of example 1) is cooled to 0 ° C and added to it , 28 ml (0.002 mol) of triethylamine. The reaction mixture is stirred for 10 minutes and 0.846 g (0.002 mol) of the product described in part B is added to it, then 0.270 g (0.002 mol) of oxybenzothiazole and 0.412 g (0.002 mol) of dicyclohexylcarbodiimide The resulting mixture was stirred at 0 ° C for 2 hours and at room temperature for 48 hours. After being cooled again to 0 ° C, the mixture was filtered and filtered. The solution is concentrated in vacuo. The residue is dissolved in ethyl acetate and the solution is washed successively with IN solution of sodium bicarbonate, water, cold 0.75 N citric acid and water. The organic phase is dried over magnesium sulfate, filtered and the filtrate is concentrated in vacuo to give 1.6 g crude product. The product is dissolved in chloroform and placed on two plates for analysis by thick-layer chromatography. The reaction is carried out in a mixture of chloroform and methanol (9: 1). The main bands are cut out from each plate and the product is drawn from silica gel, extracted with a mixture of chloroform and methanol. The eluate (1.3 g) was dissolved and reapplied to a single plate for thick-layer chromatography to obtain 1.0 g (70%) of the target compound as an amorphous solid) л1 -25.6 ° (C 0.5, methanol ). Found C, 58.60; H 6.87; N 11.53. CssHsoNfiOeS (714.88). Calculated,%: C 58.80; H, 7.05; N 11.76. G. L-tyrosyl-D-alanyl-L-alanyl-L-phenylalanyl-L-methyl-1-methionylamide hydrochloride monoacetate. A mixture of 5 ml of glacial acetic acid containing 0.5 ml of anisole and 0.880 g (0.0011 mol) of the product described in Part B was bubbled with hydrogen chloride for 30 minutes, then the reaction mixture was cooled and lyophilized. Obtain 0.704 g of the target compound - -16.2 ° (C 0.5, methanol). Found,%: C 53.48; H 6.47; N 11.62; every 6.50. CjoH, g06 5-1.25 HE-CrH O, (719.14) Calculated,%: C 53.43; H 6.45-, N 11.68; Ct 6.16. Amino Acid Analysis: Found Tug 1.00; AEa 1.99-, Phe 1.01. Example 6. Preparation of 1-tyrosyl-0-alanylglycyl-1-phenylalanyl-1--methyl-5-ethylcystaneNILamide acetate. A. Dicyclohexylamine salt of N-tert-butyloxycarbonyl-5-ethyl-1-cysteine. 50 g (0.336 mol) of 1- (5-ethy-1) -cysteine and then tetramethylguanidine (44.8 ml, 0.336 mol) and dicyclohexylamine (b6.8 ml, 0.336 mol) are added to 400 ml of dimethyl forms and . Thereafter, azidomurate acid tert-butyl ester (68 ml, 0.50 mol) is added dropwise with stirring over 1 hour and the mixture is stirred for 48 hours at room temperature. The dicyclohexyl ammonium azide precipitated was filtered off and the filtrate was evaporated in vacuo. Remainder
    partitioned between ether and water. The pH of the aqueous layer is adjusted to 8.0. The organic layer is separated and discarded. The aqueous layer is acidified to pH 2.0 with cold dilute hydrochloric acid and extracted with cold ethyl acetate. The ethyl acetate phase is washed with water, dried over magnesium sulfate and concentrated in vacuo. The resulting residue is dissolved in ether and 66.8 ml (0.336 mol) of dicyclohexylamine is added to the solution. The precipitate obtained is recrystallized from ethyl acetate to obtain 32.8 g (23% of theory) of the desired compound; m.p. 156-159 ° С, 1 ° (С 1.0, methanol), -7.7 (С 1, methanol) .®
    Found,%: C 61.37; H 9.98 / N 6.26.
     (430.6).
    Calculated,%: C, 61.36; H, 9.83; N 6.51.
    B. M-Butyloxycarbonyl M-methyl-S-ethyl-L-cysteinyl amide.
    18.58 g (74.3 mol) of N-butyloxycarbonyl-S-ethyl-L-cysteine (prepared by neutralizing the product described in Part A. and extracting with ethyl acetate) were added to 50 ml of anhydrous tetrahydrofuran. The resulting mixture is added dropwise over 30 minutes to a stirred suspension of 42.45 g of potassium hydride (22.1% KN in mineral oil 0.234 mol KN) in 375 ml of tetrahydrofuran at 0 ° C and containing 0.35 g of 18-crown -6-ether. Then methyl iodide (9.25 ml, 0.149 mol) in 20 ml of tetrahydrofuran is added dropwise within 15-20 minutes. The mixture is stirred at 1.5 hours, 7.5 ml of acetic acid in 7.5 ml of tetrahydrofuran and then 5 ml of ethanol are added dropwise to it. The resulting reaction mixture is poured onto ice and the pH is adjusted to 9 by adding 2 N sodium hydroxide solution. The resulting aqueous solution is extracted with ether, the pH of the 3 aqueous layer is adjusted by adding solid citric acid, then extracted with ether (3x300 ml). The ether extracts are combined, washed with water, dried over magnesium sulfate, and concentrated in vacuo. The resulting residue is dissolved in 200 ml of ether and to the solution is added 9.56 ml (74.3; Gmol) of d- (+) -L-methylbenzylamine. 500 ml of petroleum ether are added to the cooled mixture. The solution is concentrated in vacuo and the residue is redissolved in petroleum ether. The mixture is then cooled to and the precipitate is filtered off (2.74 g). The mother liquor was concentrated in vacuo and the residue was dissolved in ether. The ether solution is washed with IN with citric acid, water, and dried with HCl / Mg sulfate, concentrated in vacuo. This gives 6.56 g (33% of theory) of iC rt syrup about -61.1 (c 1, ethanol);
    NMR (CDCSj), rf 2.90, N CHj, l, -butyl; f 4.9-4.5 CH. 5. The product (6.5 g, 0.025 mol) is dissolved in 80 ml of dimethylformamide and the solution is cooled to. Then isobutyl chloroformate (3.6 ml,
    Q 0.027 mol) and N-methylmorpholine
    (2.99 ml, Oj027 mol). The reaction mixture is stirred for 10 minutes at and then anhydrous ammonia gas is passed through it for 1 hour. The mixture is stirred for 4 hours at -15 ° C and then poured into a mixture of ice and 1 N sodium bicarbonate solution. The cold aqueous layer was extracted with ether, the ether extract was washed with cold 0.75 N citric acid and water, dried over magnesium sulfate, and concentrated in vacuo. A residue is obtained, which is crystallized from a mixture of tea leaves and petroleum ether to obtain 1.7 g.
    5 (26%) of the title compound;
    m.p. 56-59 ° C, -127.6 ° (C 0.5, chloroform).
    NMR (CH 2): CP 2.80, N-CH ,, cf 1.46 mpenr-butyl; (G 4,9-4,5;
    n V-CH.
    Found,%: C 50.56; H 7.93; N 10.51.
    C, (261.36).
    - Calculated,%: C 50.55; H 8.10; N 10.72.
    B. M-tert-Butyloxycarbonyl-1-phenyl alanyl-No. -Methyl-S-ethyl-L-cyst N. IL-amide.
    To 20 ml of glacial acetic acid containing 1 ml of triethylsilane and 4 ml of anisole, 2.5 g (9.5 mol) of () ° tert-butyloxycarbonyl-M-methyl-5-ethyl-L-cysteinyl amide are added. Dry hydrogen chloride was passed through the resulting mixture for 30 minutes, then ether was added to give a precipitate as the hydrochloride salt (1.8 g). The precipitate is dissolved in 25. 1-liter of dimethylformamide, the solution is cooled to 0 ° C and neutralized with 1.31 ml of triethylamine. The addition) / zgl-butyloxycarbonyl-1-phenylalanine (2.65 g, 0.01 mol) is added; 1.35 g (0.01 mol) of oxybenzothiazole and 2.06 g (0.01 mol) of dicyclohexyl carbodiimide. The resulting mixture was stirred at 0 ° C for 2 hours and at room temperature for 24 hours. It was then cooled to 0 ° C and the precipitate formed was filtered off. The filtrate was concentrated in vacuo and the resulting residue was dissolved in ethyl acetate. The solution is extracted with 1 N sodium bicarbonate solution, water, 0.75 N citric acid, and water. The mixture is then dried 5 over magnesium sulfate, the solvent
    evaporated in vacuo, the resulting syrup was dissolved in chloroform and the solution was introduced into a column (3x45 cm) containing Grade and Davidson grade 62 silica gel. Elution is carried out with a mixture of chloroform and methanol under a step gradient of Cti, - CHCEj (CHjOH) C9: I, the fractions are combined according to the results of the analysis and evaporated in vacuo to obtain 3.0 g of the target compound; (C 0.5, methanol).
    Found,%: C 58.87 / H 7, N 9.81.
    CjbHsifjOl (409.5).
    Calculated,%: C 58.65; H 7.63; N 10.26.
    G. Dicyclohexylamino salt of N -pret-butyloxycarbonyl-1-tyrosyl-O-alanylglycine.
    46.80 g of the product obtained by hydrogenolysis (as described in Part D of Example 1), benzyl N -mpem-butyloxycarbonyl-0-benzyl-L-tyrosyl-O-alanyl glycinate, is dissolved in 150 ml of isopropyl alcohol and added to the solution 16 ml (0.081 mol of dicyclohexylamine) and ether, bringing the volume of the solution to 1.5 liters, evaporated to a semi-solid mass, triturated until solidified, and the resulting residue is dried. 46.04 g (98%) of solid are obtained; mp. 194 , 5-197®С
    The substance is dissolved in 100 ml of boiling methanol and 500 ml of isopropyl alcohol is added. The solution is evaporated to 150 ml, blowing nitrogen through it. After cooling, the product precipitates, the precipitate is separated, dried and 41.44 g (88%) of the title compound are obtained; m.p. 198-200.5 s 17.9 ° (C 1, methanol).
    Found,%: C 62.95; H 8.77; N 9.20.
    , 07 (590.8).
    Calculated: C 63.03; H 8.53; N 9.48.
    3. N - lDpen1-Butyloxycarbonyl-L-tyrosyl-0-alanylglycyl-L-phenyl. RIalanyl-N-methyl-S-ethyl-L-cysteinyl imide.
    2.5 g (6.1 mol) of the product described in Part B are added to 20 ml of glacial acetic acid containing 3 ml of anisole and 3 ml of triethylsilane. Dry hydrogen chloride is bubbled through the reaction mixture for 25 minutes. the ether, the mixture is cooled, the precipitate is filtered off and 1.9 g (5.5 mol) of hydrochloride salt are obtained. The salt is dissolved in 25 ml of dimethylformamide, the solution is cooled, and 3.2 g (5.5 mol) of the dicyclohexylamine salt AND-trep-butyloxycarbonyl-L-tyrosyl-D-alanylglycine are added to it. The resulting mixture is stirred at 0 ° C for 10 minutes . Then hydroxybenzothiazole (0.74 g, 5.5 mol) and dicyclo-hexylcarbodiimide (2.1 g, 5.5 mol) are added.
    and the mixture is stirred at 2 hours and at 4 ° C - 48 hours. The formed precipitate is filtered off and the filtrate is evaporated in vacuo. The residue was dissolved in ethyl acetate, and the solution was washed with a 5 in catr bicarbonate solution, water, 0.75 N citric acid, and water. The organic phase is dried over magnesium sulphate and evaporated in. 3.5 g of a crude compound are obtained. The product is dissolved in chloroform and added to a 3x45 cm column with silica gel of Grade 62, firms Grace and Davidson, and the elution is carried out under a step gradient with a mixture of chloroform-methanol 5 cHCEj-CHCtjiCH OH (9: 1). the fractions are combined on the basis of the results of the analysis by chromatography in a thin layer, evaporated in vacuo. Obtain 2.4 g (62%) of the pure target compound; t y -30, 7 (C O, 5, methanol).
    Found,%: with 58,14; H 6.96 N 11.94.
    Ca H «Ng08S (700,86). C, 58.27; H 6.90; 5 N 11.99.
    Amino Acid Analysis: Naideio Tug 1.01; Aea 1.00; sew 1.00; Phe 0.98; NH 1.09.
    E. Acetate L-tyrosyl-O-shanilgly. Cyl-1-phenylalanyl-1-S-methyl-5-ethyl-cysteinyl amide.
    To 20 ml of glacial acetic acid, containing 2 ml of anisole and 2 ml of triethylsilane, 2.2 g (Smol) of the product described in part D are added. Dry hydrogen chloride is bubbled through the reaction mixture for 25 minutes. Ether is added and the mixture is cooled. The resulting precipitate was filtered and dried (2.0 g). 0 The precipitate (1.2 g) is dissolved in a sufficient amount of buffer (1% pyridine and 0.05% acetic acid in water) to achieve a volume of 10 ml. The solution is added to a 2.5x99 cm column filled with diethylaminoethyl - Sephadex A 25 (acetate), pre-equilibrated with the same buffer. Certain fractions are combined and lyophilized. When repeated (Lyophilisation. FROM 10% acetic acid and subsequent freeze-drying from a mixture of water and acetonitrile (75:25), 0.59 g of the desired compound is obtained, H +9, 9 (C 0.5, 1 N-HCG) .
    Found,%: C 56.63; H 6.72; 5 N 12.63; S 4.69.
    Cj Hj NgOgS (660.79). Calculated,%: C 53.35; H 6.71; N 12.72; S 4.85.
    Amino Acid Analysis: Found Tug (1.00; AEa 1.01; Gty 1.00; Phe 0.98; NH ,, 1.09.
    Example 7. Preparation of 1-triosyl-0-alanylglycyl-1-fe5nylalanyl-N -methyl-L-leucylamine acetate. A. N -i f "m-Vutyloxycarbonyl-M-methyl-1-leucine in the form of d - (- f) -d-Me of the tylbenzylamine salt. To 20 ml of ether was added 12.5 g (0.05 mol) of the hydrate M-tr "p-butyloxycarbonyl-L-leucine and the mixture was dried over magnesium sulphate and concentrated in vacuo. The residue is dissolved in 75 tetrahydrofuran and the solution is added dropwise with stirring over 35 minutes to a cooled suspension () 27.9 potassium hydride (22.1% suspension in mineral oil, 0.154 mol KN) in 200 ml of tetrahydrofuran containing 0.25 g of 18-crown-6-ether. Ketil iodide (6.4 g) in 10 ml of tetrahydrofuran is then added dropwise over 15 minutes. The mixture was kept at 0 ° C for 3 hours, then 5 ml of acetic acid in 5 ml of tegrahydrofuran and 5 ml of ethanol were added dropwise to it. The resulting mixture is poured onto 500 ml of ice while adding 1 N sodium hydroxide solution and the pH is adjusted. The aqueous solution is extracted with ether and acidified to pH 3 with the addition of solid citric acid. The acidified aqueous suspension is extracted with ether. The combined ether extracts are washed with water, dried over magnesium sulfate and concentrated in vacuo. Receive; 13.2 g (107% of theory). raw product. Chromatography analysis in a thin layer showed the presence in the resulting product of a certain amount of unreacted starting product. The product obtained is dissolved in ether and 5.25 ml (0.05 mol) of tetrabu-Thylamine is added to the solution. The ether solution is diluted with petroleum ether and cooled overnight. The precipitate formed is filtered and the filtrate is washed with 1 N citric acid and then water. The organic phase is dried over magnesium sulphate and concentrated in vacuo. The residue (6.45 g) was dissolved in 100 ml of ether and 3.39 g (0.026 mol) of d - (+) - d-methylbenzylamine was added to the solution. The solution is cooled overnight, then filtered and 9.09 (49% -) of the title compound are obtained; mp. 12,122 "s; CF G -14.1 ° (C 1, methanol). Found,%: C 65.83. H 9.05; N 7.35. CjoHjuNjO (366.5), Calculated,%: C 65, 54; H 9.35; N 7.64. B. -Butyloxycarbonyl-L-N -methyl-L-leucyl amide. A mixture of 80 ml of dimethylformamide and 11.5 g (0.047 mol) of N-rTipem-butyloxycarbolyl-M-methyl-1-leucum (prepared by neutralizing the product described in Part A with citric acid, followed by extraction with ether) is cooled to -15 ° C and isobutyl chloroformate (6.7 ml, 0.052 mol) and N-methylmorpholine (5.7 ml, 0.052 mol) are added to it. The mixture was stirred for 10 minutes at -15 ° C and infused ammonia was bubbled through for 1 hour. Stirring is continued for another 4 hours at -15 ° C. The reaction mixture is then poured into a mixture of 1 N sodium bicarbonate solution and ice. The cooled mixture is extracted with ether, the ether layer is washed with 0.75 N citric acid and water, dried over magnesium sulfate and evaporated in vacuo. The residue is crystallized from a mixture of ether and petroleum ether, to give 5.5 g (48%) of the desired compound; m.p. 127-128 ° C; - 42.2 "(C 1, methanol). Found,%: C 59.17; H 9.66; N 11.21. SdNaM Oz (244.3). Calculated,%: C, 58.99; H, 9.90; N 11.47, B. M-tert-Butyloxycarbonyl-1-phenylalanyl-M-methyl-1-leucylamine. To 30 ml of glacial acetic acid, containing 3 ml of anisole and 3 ml of triethylsilane, 5.0 g (0.02 mol) of the product described in Part B- are added, and dry hydrogen chloride is bubbled through the mixture for 25 minutes. Ether is then added and the mixture is cooled. The precipitate formed is dried (3.6 g). The collected hydrochloride salt is dissolved in 60 ml of dimethylformamide. The solution is cooled to 0 ° C and 3.99 ml (0.02 mol) of dicyclohexylamine is added to it. The mixture was stirred at 10 minutes, 5.3 g (0.02 mol) of gp / t-butyloxycarbonyl-L-phenylalanine were added, followed by 2.7 g (0.02 mol) of oxybenzothiazole and 4.12 g (0.02 g). mol) dicyclohexylcarbodiimide. The reaction mixture is stirred for 2 hours at then at room temperature for 24 hours. After cooling, the mixture is filtered and the filtrate is evaporated in vacuo. The residue was dissolved in ethyl acetate, and the resulting solution was washed with 1 N sodium bicarbonate solution, water, 0.75 N citric acid, and water. The solution is then dried over magnesium sulphate and evaporated in vacuo. The resulting residue was dissolved in chloroform and the solution was transferred to a 3 x 45 cm column with silica gel. Elution was carried out with a chloroform-meth anol j CHCEg - CHCEj:: gradient (9: 1). The fractions were combined based on the results of a thin layer chromatography and after evaporation of the solvent, 5.7 g (73%) of the desired compound were obtained; Г -49,5 ° (С О, 5, methanol).
    NMR (COC): (G 1.4 tert-butyl / CG 7.25 phenyl) cf 0.95-0.75, CH (CHi). 6-2.7 N-CH,
    G.) cn) -Butyloxycarbonyl-L-tyrosyl-0-alanylglycyl-L-phenyl-alkyl-N-methyl-L-lecylamide
    A mixture of 20 ml of 1 N HC in glacial acetic acid containing 1 ml of anisole and 2.0 g of the product described in Part B was held at room temperature for 30 minutes, ether was added and cooled. The resulting precipitate was separated and dried (1.63 g). The collected hydrochloride salt was dissolved in 30 ml of dimethylformamide, and 2.95 g (0.05 mol) of the d-chlorohexylamine salt of N-P f-butyloxycarbonyl-L-tyrosyl was added to the solution. O-alanylglycine. The mixture is stirred for 15 minutes at 0 ° C, then 0.675 g (0.005 mol) of hydroxybenzothiazole and 1.3 g (0.005 mol of dicyclohexylcarbodiimide) are added to it. The reaction mixture is stirred for 24 hours at 4 ° C, the resulting precipitate is filtered and the filtrate is concentrated in vacuo. The residue is dissolved in ethyl acetate and the solution is washed with 1 N sodium bicarbonate solution, water, 0.75 N citric acid, and water. The etilacetate solution is dried over magnesium sulfate and concentrated in vacuo. The resulting residue is dissolved in chloroform and paciBOp is added to a column of 3x45 cm with silica gel. Product Eluy using a step gradient, a mixture of chloroform-ethanol, l CHCEj-C.CHEC-CH, OH (9: 1) .1 The fractions are combined on the basis of the results of the chromatography in a thin layer and after evaporation of the solvent, 2.3 g (67% ) the target compound ;;; -17.5 ° (C 0.6, methanol).
    Found 9%: C 61.33; H 7.47; N 12.08.
    CssHsoNbOfi (682.8).
    Calculated,%: C, 61.57; H 7.38; N 12.31.
    Amino Acid Analysis: Found Tug 1.00; ACa 1.01; GCy 0.99; Phe 1.00; NH 3 1.08.
    D. Acetate L-tyrosyl-O-alanylglycyl-L-phenylalanyl-M-methyl-L-leucylamide and ..
    A mixture of 5 ml of formic acid containing 0.5 ml of anisole and 0.1 mp of triethylsilane and 1.8 (0.003 moL) of the product described in part D is stirred at room temperature for 3 hours, then diluted with ether and incubated for 1 hour. . From the resulting oil, the ether is separated by decantation. The oil is dissolved in ethanol. At. a precipitate is obtained, which is filtered off and dried, and 0.9 g of the crude title compound is obtained. The product is dissolved in a sufficient amount of buffer (1% pyridine and 0.05%
    formic acid in water) to a volume of 5 ml. The solution was introduced into the column with 2.5 x 100 cm of races filled with diethylaminoethyl-Sephadex A-25 (formic acid ester) and eluted with the same buffer. The appropriate fractions are combined based on the UV elution profile and lyophilist extract. When re-lyophilization from 10% acetic acid and from a mixture of water and acetonitrile (75:25) receive. 0.852 g of the title compound (G + 23.2 "(C 0.6, NHC8).
    Amino Acid Analysis: Found 1.02; Aya 1.00; Git at 1.01; Phe 0.96j NHj 1.03.
    five
    , Example 8. The preparation of hydrochloride L-tyrosyl-O-alanylglycyl-L-phenylalanyl-S-p-methoxybenzyl-1-cysteinlamide.
    A. N-tert-Butyloxycarbonyl-S0-paramethoxybenzyl-1-cysteinyl amide.
    To 80 ml of dimethylformamide was added 6.82 g (0.02 mol) of M -tr # -butyloxycarbonyl-5-p-methoxybenzyl-L-cysteine and after cooling to
    5 -15 ° C, 2.88 ml (0.022 mol) of isobutyl chloroformate and 2.42 ml (0.022 mol) of N-methylmorpholine are added to this mixture. After 10 min through the reaction mixture in
    0 for 1.5 h bubbles pass anhydrous ammonia. Stirring is continued for another 2 hours at. Then the reaction mixture is poured into a mixture of ice and 1 N sodium bicarbonate solution. The resulting aqueous suspension is extracted with ethyl acetate and the resulting extract is washed with water, 0.75 N citric acid and water. The organic layer is dried over magnesium sulfate and concentrated in vacuo. The residue is recrystallized from a mixture of ethanol and water, and 4.9 g (72%) of the desired compound are obtained; m.p. 138-1400С; s g-12.8 ° (C 5, methanol)
    five
    Found,%: C 56.58; H 6.97; N 8.07.
    C jHyN-iO, S (340.4).
    Calculated,%: With 56,45; H 7.11; N 8.23,
    0
    B.N -mpem-Butyloxycarbonyl-L-phenylalanyl-5-p-methoxybenzyl-L-cystenylamide.
    Anhydrous hydrogen chloride is bubbled through a solution of 4.1 g.
    5 (0.012 mol) of the product described in part A, in 45 ml of glacial acetic acid, 5 ml of anisole and 5 ml of triethylsilane. After 20 minutes, ether was added, the precipitate formed (3.3 g) was separated and dried. Collected
    The hydrochloride salt is dissolved in 50 ml of dimethylformamide, and 2.92 g (0.012 mol) of 1-cyclohexylamine, 3.19 g of 0.012 mol) m-tr1-butyloxycarboxy-nyl-1-phenylalanine and 1.62 (0.012 mo of oxybenzothiazole are added to the solution. The mixture was stirred for 10 minutes with 2.47 g (0.012 mol) of dicyclohexylcarbodiimide was added. After stirring for 2 hours while the reaction mixture was stirred for another 24 hours at room temperature, then cooled again and the precipitate was filtered off. The filtrate was concentrated in vacuo and the residue was dissolved in n-butyl alcohol. The solution is washed with 1 N Sodium bicarbonate and water are then dried over magnesium sulphate and evaporated in a vacuum.The resulting residue is crystallized from ethanol and 4.95 g (85%) of the title compound is obtained 17 178 ° C; D -35.1 ° (C - 0, 5, dimethylformamide) Found,%: C 61.78; H 6.78; 8.28. C HjeNjOsS (487.6) Calculated,%: C 61.58 H 6.82; N 8.62. B. N tert-butyloxycarbonyl L-tyrosyl-D-alanylglycyl-L-phenyl. Aryl-5-p-methoxybenzyl-1-cysteine amide. Anhydrous hydrogen chloride bar is botaned through a solution of 1.3 g (0.027 mol) of the product described in Part A in 40 ml of glacial acetic acid, 4 ml of anisole and 4 ml of triethyl silane. After 20 minutes, ether was added to the mixture, and the resulting precipitate was collected and dried (1.1 g). The chloride of the prenatal salt is dissolved in 10 ml of dimethylformamide and the mixture is cooled to. Triethyles (0.34 ml, 0.0026 mol) are then added and after 10 1.06 g (0.0026 mol) M-tert-butyl oxycarbonyl-1-tyrosyl-D-alanylglycine, 0.35 g (0 , 0026 mol) oxyben zothiazole and 0.536 g (0.0026 mol) of dicyclohexylcarbodiimide. The reaction mixture is stirred at 2 hours and at 72 ° C for 72 hours. The precipitate is collected and the filtrate is concentrated in vacuo. The residue was dissolved in ethyl acetate and the solution was washed with 1 N sodium hydroxide solution, water, O, 75 N citric acid and water. The extract is dried over magnesium sulfate and concentrated in vacuo. The residue is dissolved in ethyl acetate and purified by chromatography on a gel. The fractions were combined by analysis, concentrated, and 1.1 g (52%) of the desired compound were obtained after crystallization from a small volume of ethyl acetate / Jj l-4.3 (0 0.5, dimethyl sulfoxide). Found,%: C 59.95, H 6.24; N 10.53. (778.9). also,%: C 60.14; H 6.47, N 10.79. Anashine amino acids: Found Tug 0.98; Ava 1.03; GEy l, Oi; Phe 0.98; NHj 0.99. G. 1-Tyrosyl-0-alanylglycyl-1-phenylalanyl-5-p-methoxybenzyl-1-cysteinylamide hydrochloride. To 20 g of glacial acetic acid containing 0.5 ml of anisole, 0.90 g (0.0012 mol) of the product described in part B is added. Anhydrous hydrogen chloride is bubbled through the solution for 30 minutes. The mixture is then lyophilized, and 0.862 g (100%) of the desired compound is obtained; oll + 2.6 ° (C 0.5, 1NHCE) Found,%: C 56.85, H 6.06, 11.48; SS 5.21. N g O 7 S (715.2) Calculated,%: C 57.09; H 6.06; N 11.75 C) 4.96. Amino Acid Analysis: Found 0.99; Aya 1.01; seu i, oi; Rie o, 98; NHj oh, 99. Example 9. Preparation of L-tyrosyl-D-alanylglycyl-L-phenyl alanine-M-methyl-1-methionyl amide acetate. A. Sol N -mpem-butyloxycarbonyl-M-methyl-1-methionine and d (+) d-methylbenzylamine. A suspension of the d-cyclohexylamine salt N-gn / eat-butyloxycarbonyl-L-methionine (86.13 g, 0.2 mol) in 600 ml of cold ether is washed 4 times in 100 ml portions with cold 1.5 N citric acid and water. The separated organic phase is dried over magnesium sulphate and concentrated in vacuo. The residue is dissolved in 150 ml of tetrahydrofuran and the solution is added dropwise over 30 minutes to a stirred suspension of 0.6 mol of potassium hydride in 1000 ml of anhydrous tetrahydrofuran () containing 1.0 g of 18-crown-6-ether. Methyl iodide (25 ml, 0.4 mol) is added dropwise to the reaction mixture over 15 minutes. After 2 hours, a mixture of 20 ml of acetic acid and 20 ml of tetrahydrofuran was added dropwise, and then 40 ml of ethanol. The mixture is stirred for 30 minutes and poured onto 2 liters of ice. Upon addition of 2N potassium hydroxide solution, the pH is adjusted to 7 with an aqueous mixture and the product is extracted with ether (3 x 400 ml), then acidified to pH 3 with solid citric acid. The mixture is extracted with ether (3x500 ml). The ether extracts are combined, washed and dried over magnesium sulfate, evaporated in vacuo to obtain a syrup (44.76 g, 84% of theory). The syrup is dissolved in 450 ml of ethyl acetate and 25.78 ml (0.2 mol) is added d - (+) - d-methylbenzylamine. The mixture is cooled, the precipitated product is filtered off and 51.05 g (66%) of the title compound are obtained; m.p. 131-134 °; tfl -18.9 ° (C 1, ethanol).
    Found,%: C 59.15; H 8.12; N 7.29.
    C gHjuNjO S (384.54), Calculated,%; C, 59.35; H 8.39; N 7.29.
    B. N-p L1-Butyloxycarbonyl-N -methyl-1-methionylamide.
    M-tert-Butyloxycarbonyl-K-methyl-1-methionine (33.3 g, 0.127 mol), obtained by acidifying the d - (+) - d-methylbenzylamine salt described in Part A, is dissolved in 160 ml of dimethylformamide and extracted The solution is cooled before and 18.3 ml (0.14 mi) of isobutyl chloroformate (15.4 ml, 0.14 mol) of N-methylmorpholine are added to it. The mixture is stirred for 10 minutes and anhydrous ammonia is bubbled through it for 1 After stirring at -15 ° C, it is continued for 4 hours, then the reaction mixture is poured into 300 ml of a cold solution of IN sodium bicarbonate. The aqueous suspension is extracted with ether. The ether extract is washed with water, cold 0.75 N citric acid and water, dried over magnesium sulfate and evaporated in vacuo to a syrup. The syrup is crystallized from a mixture of ether and petroleum ether to obtain 16 g (48%) of the desired compound; m.p. 75-77 ci tdvli - 177.3 ° (C 0.5, chloroform).
    Found,%: C 50.63; H 8.57; N 10.45.
    C NggMLZO-} (262,17) Calculated,%: C 50.36; H 8.45; N 10.78.
    B. m-p1re1 -Butyloxycarbonyl-1-phenylalanine-M-methyl-1-methionylamide
    Anhydrous hydrogen chloride was bubbled through a mixture of 70 ml of glacial acetic acid, 5 ml of anisole, 7 ml of triethylsilane and 13.15 g (0.05 mol) of the product described in part B, and it was poured onto the ether. The precipitate formed (9.9 g dried. The hydrochloride was dissolved in 200 ml of dimethylformamide. The mixture was cooled to 0 ° C and 9.9 ml (0.05 mol) of dicyclohexylamine was added. After 10 minutes of stirring, 6.8 g (0 , 05 mol) oxybenzothiaeol, 13.3 g (0.05 mol) N-tert-butyloxycarbonyl-1-phenyl sshanin and 10.3 g (0.05 mol) dicyclohec-silcarbodiimide The mixture is stirred for 2 hours at 48 h and at room temperature. After cooling, the mixture is filtered and the filtrate is concentrated in vacuo. The resulting oil is dissolved in ethyl acetate and the solution is washed successively with a 1 N solution. m sodium bicarbonate, water, O, 75 N citric acid and water. The ethyl acetate solution is dried over magnesium sulfate and evaporated in vacuo to give a residue, which is crystallized from ether and 16.4 g (80%) of the desired compound are obtained; 114-115 s; - 43.4 (C 0.5, methanol).
    Found,%: C 58.76; H 7.42; N 10.30.
    CjoH}, NjO | S (409.55)., 5 Calculated,%: C 58.65; H 7.63; N 10.26.
    G. N-tert-Butyloxycarbonyl-L-tyrosyl-0-apanylglycyl-1-phenylalanyl-N-methyl-L-methionylamide. 3.5 g (8.56 mol) of the product described in Part B are added to a mixture of 20 ml of glacial acetic acid, 2 ml of anisole and 2 ml of triethylsilac, and anhydrous hydrogen chloride is bubbled through the mixture for 25 minutes.
    5 Then ether is added, the precipitated hydrochloride is filtered off and the filtrate is dried under vacuum. A solution of 5.0 g (8.47 mol) of the salt of N -mpem-butyloxycarbonyl-1-tyrosyl-0-alanylglycine 0 and dicyclohexylamine in 40 ml of dimethylformamide is cooled to and the previously obtained hydrochloride salt is added to the solution. After stirring at 0 ° C for a few 5 minutes, G, 1 g (8.47 mol) is added.
    oxybenzothiazole and 1.7 g (8.47 mol), dicyclohexylcarbodiimide. The mixture is stirred for 24 hours at. The resulting insoluble product Q is filtered off and the filtrate is evaporated in vacuo. The residue was dissolved in ethyl acetate, and the solution was washed successively with a 1 N aqueous solution of sodium bicarbonate, water, cold with 0.75 N citric acid and water. The solution is dried over magnesium sulphate and evaporated in vacuo. The residue is chromatographed as described in the previous examples; 4.1 g (69%) of the desired compound are obtained; -13.1 ° 0 (c 0.5, methanol).
    Found,%: C 58.05, H 6.62; N 11.73.
     (700.86).
    Calculated,%: C 58.27; H 6.90; 5 N 11.99.
    Amino Acid Analysis: Found Tug 1.00; Ala 1.01; GEy 0.99; Phe 1.00; NHj 1.01.
    D. Acetate L-tyrosyl-O-alanylglyl Cyl-1-phenylalanyl-S-methyl-L-methioyl amide.
    A mixture of 15 ml of thioanisole, 8.3 g (0.012 mol) of the product described in part D, is cooled to 0 ° C and 50 ml of cold trifluoroacetic acid is added to it. The mixture was stirred at 30 minutes, then diluted with several volumes of ether. The precipitate obtained is boiled; 8 g of crude trifluoroacetic acid salt is obtained. The salt is dissolved in a sufficient volume of aqueous buffer containing 1% pyridine and 0.05% acetic acid to bring the total volume to 60 ml. The solution is transferred to column 5 (5x138 cm) from diethylaminoethyl sepha.
    权利要求:
    Claims (2)
    [1]
    Claim
    1-Method for the preparation of peptides of the general formula
    OR 8 OR 7 '
    I-CH-C — H — CH — C — IN — CH — C — I — CN — C — N
    A s
    1 CHj ~ cn-cc cn, I w where R ^ R ^ R * is hydrogen;
    R 8 - C4 - C4 - alkyl 1
    R s "R e * R 7 is independently hydrogen or methyl,
    [2]
    2 = - with -HH,. and g 'o g
    w = —CH a scHj, -sn (ssg) g , -pcHjCHj or their salts, characterized in that in the compound of the formula
    OH
    OR 3 0
    II I II
    OR 6 OR 7
    II I II I
    C-H-CH- Z I CHz
    V where o '. - tert-butyloxycarbonyl, Z and W have the above meanings, the protective groups are cleaved off in an anhydrous acidic medium.
    2. The method according to claim 1, characterized in that the process is carried out in trifluoroacetic or glacial acetic acid with gaseous hydrogen chloride or in 98% formic acid.
    Priority by signs:
    09/27/76 at R <= k r = i 3 - hydrogen; ' R * -C <-C4 alkyl;
    40 R = R 6 = R 7 is hydrogen or methyl;
    W = —CH t —S — CH 3 ;
    Z = - С - НН,,
    II g 'o
    06/20/77 at W = -CH (CH 3 ) 2 ;
    - S - CH g CH
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    引用文献:
    公开号 | 申请日 | 公开日 | 申请人 | 专利标题
    GB1532181A|1976-02-02|1978-11-15|Beckman Instruments Inc|Pentapetides and a method of preparing them|
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    DE2933947A1|1979-08-22|1981-03-12|Hoechst Ag, 6000 Frankfurt|NEW PEPTIDAMIDES AND METHOD FOR THEIR PRODUCTION.|
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    法律状态:
    优先权:
    申请号 | 申请日 | 专利标题
    US72672476A| true| 1976-09-27|1976-09-27|
    US05/807,849|US4259234A|1976-09-27|1977-06-20|Analgesic compounds|
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