• 专利附录
  • 专利说明
  • 权利要求
  • 类似技术
  • 同族专利
  • 引用文献
  • 法律状态
  • 优先权
    • 专利摘要:
    Glucose is converted to fructose in the presence of an enzyme produced by Flavobacterium arborescens.
    公开号:SU747436A3
    申请号:SU772540996
    申请日:1977-10-19
    公开日:1980-07-23
    发明作者:Ки Ли Чин
    申请人:Р.Дж. Рейнольдс Тобакко Компани (Фирма)；
    IPC主号:
    专利说明:

    one
    The invention relates to the food industry, in particular to a method for producing fructose by enzymatic glucose isomerization.
    A known method for producing fructose is by treating a glucose solution with a glucose enzyme preparation from bacteria, followed by isolation of the final product i.
    The lack of a known method is the low yield of fructose.
    The purpose of the invention is to increase the yield of fructose.
    This goal is achieved by using the F avobac teriura arborescens ATCC 4358 or NRRL B - 11022 or NRRL B - 11023 as producers of glucose isomerase.
    For the production of fructose, whole microbial cells, immobilized cells, as well as a cell-free extract containing glucose isomerase are used. Regardless of the forgs in which the glucose isomerase is used, the process of isomerization of glucose into fructose is carried out at 60-75 ° C, pH 6.5-8. Isomerization is carried out both in a solution of pure glucose and in a solution of starch hydrolysates obtained by acid or enzymatic treatment. The glucose concentration in the solution is 30-60%.
    Enzyme activity is increased by adding small amounts of magnesium salts to the glucose solution.
    Characterization of F strains of avobacterium arborescens ATCC-4358, NRRL B-11022, NRRL B-11023.
    Stored in a permanent collection
    10 cultures of the ARS Northern Regional Research Laboratory of the US Department of State in Peoria, Illinois.
    The rods are 6.5-2.5 microns, arranged alone and with the formation of chains. Long, wavy filaments form in the broth. Motionless, greggy positive. Colonies on gelatin: colonies at first fibrous
     and the branch is, when observed, with a small increase. The centers become yellowish, and the edges become translucent and branching. Sowing a gelatin injection: thinning to form a yellow precipitate. Laying out on beveled agar: slow growth, the color is warm orange. Broth: turbid, yellow precipitate; thin films are missing. Litmus milk:
    30 slow coagulation, recovery
    litmus The reaction is neutral. Cartrfel: lush growth, bright orange rubs. Growth is absent in the nitrate solution; nitrites are not produced. Aerobic, optionally anaerobic, optimal growth temperature.
    The strains differ in the level of glucose isomerase activity depending on the carbon source. The ATCC 4358 strains, NRRL 11022, NRRL B - 11023 pro is the maximum glucose isomerase a | (when grown on a nutritional medium containing xylose or lactose as the sole source of carbon
    Gomer 1. To 500 ml of 50% glucose solution containing MgCEj B; a concentration of 0.01 M, 10 g of washed cells FEavobacterI urn arborescens NRRL B - 11022, grown on a nutrient medium containing lactose, as a carbon source, are added. The solution is alkalinized with NaOH to pH 8.0 and incubated for 20 hours at 60 ° C and weak stirring. The yield of fructose 45%.
    ; Example 2. To 500 ml of 50% placTBOpa glucose containing MDSE: O concentration, 005 M, add 9; g cells FSavobacterium arboresce NRRL B - 11023 grown on a nutrient medium containing glucose as a carbon source. The solution is alkalinized with NaOH to pH 8.0 and incubated for 27 hours at 60 ° C and (pub stirring. Fructose yield 3S%.
    P. and Mp 3. The process is carried out according to example 1, but using
    Ftavobactag cells; looking for arborescens ATCC 4358 grown on nutrient medium containing xylose as a carbon source. After 24 hours incubation, the yield of fructose is 35%.
    Example 4. A 50% glucose solution containing MgCEj in a concentration of 0.005 M, at pH 8.0, is passed through a 1 inch C glass column with 5 g of avobacterium arborescens F cells N avobacterium arborescens NRRL B - 11022 immobilized by flocculation grown on a nutrient containing lactose as carbon source Liquid consumption 26 ml / h. The yield of fructose 45%.
    Thus, the use of glucose isomerase for the isomerization of glucose to fructose from the described F strains of avobactegium arborescens makes it possible to obtain fructose with a good yield.
    Formula
    the invention
    The method of producing fructose by treating a glucose solution with an enzyme preparation of glucose isomerase from bacteria, followed by isolation of the final product, and with the fact that, to increase the yield of the target product, strains of G avobactegium arborescens ATSS38 are used as producers of glucose isomerase. or NRRL B-11022, or NRRL B-11023.
    Sources of information taken into account in the examination
    1. US patent K 3829369, cl. 195-31, pub. 1976 (prototype
    权利要求:
    Claims (1)
    [1]
    Claim
    The method of obtaining fructose by
    25 treatment of a glucose solution with an enzyme preparation of glucose isomerase from bacteria, followed by isolation of the final product, characterized in that, in order to increase the yield of the target product, strains of G Avobactegium arborescens ATCC 4358 or NRRL B-11022, or NRRL B-11022, or NRRL B-11022 are used 11023.
    35 Sources of information taken into account in the examination
    1. US Patent K 3829369, cl. 195-31, published. 1976 (prototype).
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    同族专利:
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    引用文献:
    公开号 | 申请日 | 公开日 | 申请人 | 专利标题
    JPS5311929B2|1972-08-31|1978-04-25|
    US3834988A|1972-10-10|1974-09-10|Anheuser Busch|Method of making glucose isomerase and using same to convert glucose to fructose|
    GB1455993A|1973-01-12|1976-11-17|Novo Industri As|Glucose isomerase|
    US3956066A|1973-08-16|1976-05-11|A. E. Staley Manufacturing Company|Glucose isomerizing enzyme|US4399222A|1979-08-06|1983-08-16|A. E. Staley Manufacturing Company|Carbohydrases from acidophilic streptomyces|
    US4459354A|1979-08-06|1984-07-10|A. E. Staley Manufacturing Company|Carbohydrases from acidophilic streptomyces|
    US4348481A|1979-11-29|1982-09-07|Institute Po Microbiologia|Method of obtaining glucose isomerase|
    US4348480A|1980-06-04|1982-09-07|Miles Laboratories, Inc.|Process for producing glucose isomerase|
    US4317883A|1980-12-03|1982-03-02|Institute Po Microbiologia|Method for obtaining of glucose isomerase|
    US4429044A|1982-09-28|1984-01-31|Miles Laboratories, Inc.|Preparation of an alkaline protease from flavobacterium arborescens|
    US4920052A|1988-02-05|1990-04-24|Miles Inc.|Process for producing glucose isomerase|
    法律状态:
    优先权:
    申请号 | 申请日 | 专利标题
    US05/734,222|US4061539A|1976-10-20|1976-10-20|Preparation and use of glucose isomerase|
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