Milk flow controlling method

专利摘要:
An injectable sustained release formulation of bovine somatotropin comprises a mixture thereof with a wax and an oil.
公开号:SU1683493A3
申请号:SU4028010
申请日:1986-08-19
公开日:1991-10-07
发明作者:Харри Фергасон Томас；Гаррик Харрисон Роджер；Ли Мур Девид
申请人:Эли Лилли Энд Компани (Фирма)；
IPC主号:

专利说明:

The invention relates to agriculture, namely to veterinary medicine.
The purpose of the invention is to increase the milk and efficiency of the method.
For example, 1800 g of sesame oil is heated to a temperature of about 100 ° C and 200 g of refined white beeswax is added to it. The mixture is stirred at a constant temperature until the wax dissolves in the oil, and then the mixture is cooled with constant gentle stirring to 22 ° C. The oil / wax mixture is homogenized with an Ultra Turrax homogenizer until the mixture becomes homogeneous and with a cream consistency, and its viscosity decreases.
To a portion of 13.6 g of this oil-wax mixture, with weak stirring at 20 ° C, add 2.83 g of bovine somatotropin synthesized by means of a modified culture of Escherihia coti, as described in European Patent 0095361. This somatotropin preparation is absolutely pure, containing about 75% of the total protein, which is determined by changing the optical density of the dissolved material, of which about 95% is monomeric bovine kamatotropin and its related substances (determined by exclusion chromatography). This preparation contains 12.6% bovine somatotropin and related substances.
Example 2-6. The examples described below were carried out in the same manner as Example 1. The compositions of the obtained preparations are listed in Table 1.
Test 1 Preparations according to the present invention were tested on lactating cows, giving about 56 pounds (25 kg) of milk per day at the beginning of the test. Used
cl
with
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with
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with
the test group and the control group, which included 7 cows each. One control cow was removed due to the disease of hardening of the glands and two test cows were removed from the test due to the respectively solidification of the glands and mastitis, the Cows were kept indoors in the stall on the ground every day, and then were outdoors, and during this time the stall was cleared in them the litter was replaced.
The cows received a food ration (without limitation) consisting of 60% of forage (3 parts of corn silage and 1 part of crushed alfalfa hay) and 40% of concentrate, calculated on the dry weight.
A portion of the concentrate of this diet had the following composition,%.
Dried beet pulp10,00 Crushed corn48.50
Soybean Meal33,10
Known to 1.65
Cane sugar molasses 3.50 Sodium Primary Acid Phosphate 1.05 And a mixture of vitamin A, Dz and E0.35 is similar
Selenium initial mix0.05
Sodium bicarbonate 0.75
Traces of salts1.05
The cows were milked twice a day at intervals of about 11 and 13 hours. Milk production was recorded and milk samples were analyzed in the usual way. After a 14-day period of such conditioning, 7.3 g of the preparation corresponding to Example 5 was injected into the cows by subcutaneous injection, the injection of this preparation was carried out with the help of 14-gauge needles. On the 29th day after the first injection, animals were injected into the body. 7.3 g of the preparation corresponding to example 6 was injected and then the third preparation of the preparation corresponding to example b was carried out in the amount of 7.3 g; this third injection was performed on the 57th day after the first injection.
Thus, this experiment included three 28-day periods of prolonged release of the drug.
The release of somatotropin from the injection of the drug was recorded by replacing the concentration of bovine somatotropin in the blood plasma of treated cows. Blood samples were taken by puncture of the strap vein or the coccygeal vein on Mondays and weekends of each week, and these samples were analyzed by antibody double radioimmunoassay.
The results obtained for each three 28-day periods were averaged, tp
the preparation of Table 2, the average analysis data are presented in nanograms per milliliter of somatotropin. The results of the control test are not given in
Table 2, since they are within very narrow limits, in all cases from 2.9 to 3.3 ng / ml.
As expected, the milk yield of the treated cows was significantly higher and
0 The composition of this milk was normal.
Test 2 In this test, the treated and control groups were used, each of which included three cows each. The preliminary care and feeding of the animals was carried out in the same way as in Test Example 1, and the test results were determined by analyzing the content of somagotropin in the blood plasma in the same way as in
In the example of test 1, the test was carried out for 29 days after administration by injection of the preparation according to example 4, in an amount of 7.3 g per cow. The results are shown in table 3. In this
In test 5, there was a significant variability in the content of somatotropin in the body of cows in the control group; therefore, the data for the control group of cows are shown in Table 3.
0 Testing This test was performed in the same way as Test 1, with the difference that each test group included only three cows, There was no control group, but
5 to control the experiment, pretreated samples were taken and analyzed. The preparations of Examples 1, 2 and 3 were used and 4.9 g was injected into the body of each animal.
0 of the corresponding drug on day 0 of the experiment.
During this experiment, blood samples were taken from animals on different days and analyzed for the content of bovine
5 somatotropin, while the results listed in Table 4 were obtained.
At each experimental site, cows were introduced into the experiment in three different periods of their lactation. One cow body was in the early stage of the lactation period, approximately 29 to 42 days after the start of lactation. These cows were observed for 252 days of exposure and they were given 9 injections of bovine somatotans at 28-day intervals.
The second group of cows was introduced into the experiment at an average stage of lactation from 111 to 167 days after the start of lactation. These animals were observed for 168 days of exposure and they made b-injections.
bovine somatotropin with 28-day intervals.
The third group of cows at each site was introduced into the experiment at the late stage of lactation, more than 167 days after the start of lactation and when they were pregnant for about 56-126 days. These animals were observed for 84 days of exposure and they were given 3 injections at 28-day intervals.
Four different doses of bovine somatotropin, 960, 640, and 320 mg / injection were used, and non-injected control animals were observed. All 4 doses were administered at each experimental site and each cow from the groups with early, middle and late lactation. In total, the data presented covers 113 cows from the group with early lactation, 60 cows from the group with medium lactation and 66 cows from the group with late lactation.
Familiar with this data, it is necessary to remember that the milk yield of cows is constantly reduced as the lactation period increases. The quality of the milk obtained from injected cows corresponded in all respects to the quality of milk obtained from non-injected control cows.
The data obtained are summarized by calculating averages of the least squares method for all data of the first, second, third, and fourth weeks obtained over the next 28-day periods following the injections of each dose and for each lactation period. A standard error was calculated for each week data, using the corresponding effect of the statistical model of the experiment as a residual term.
The obtained data on the need for x milk ± standard error pounds / day / head are presented in Table 5 and in the graph.
Thus, compressed data provide a statistically significant picture of milk obtained from each group of cows in the middle of a 4-week period and show the ability of long-term bovine somatotropin injections to maintain increased milk yield for a 28-day period after each injection.
Each injection of bovine somatotropin increases milk yield for about three weeks. However, on the fourth week, the difference between the injected and control animals becomes lower than the sum of the corresponding standard errors.
In the groups with early lactation and with average lactation, the average milk yield for each of the first three weeks at all 3 doses is clearly higher than in control animals. If injection was started only in the period of late lactation, then a large dose of self-trotropin was required, with the highest dose allowing for a higher milk yield than in control animals for only the first 2 weeks.

权利要求:
Claims (1)
[1]
Invention Formula
A method of regulating milk production in cows, which includes administering a somatotropin-based preparation by injection, characterized in that, in order to increase milk supply, the efficiency of the method, the introduction of somatotropin is carried out in a mixture with a carrier in an amount of 10.900 - 17.699 wt.%. and as a carrier, a mixture of white or yellow beeswax with peanut or sesame oil is used in a ratio of (1-10) :( 90-99), the drug being administered during lactation 3-6 times with an interval of 28 days based on 320 - 960 mg bovine somatotropin to one animal per injection.
Table
Example
Carrier,%
Sesame oil 95 / white beeswax 5
Peanut butter 90 / yellow beeswax / 10
Peanut Butter 99 / Yellow Beeswax 1
Peanut Butter 99 / Yellow Beeswax 1
Peanut butter 90 / yellow beeswax 10
Somatotrpin,%
12.4
12.5 12.6
10.9 12.6
Table 2
Table3
Ta bl and tsa4
Table 5 Milk yield ± standard error lbs / day / head

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同族专利:

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公开号 | 公开日

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DK396286A|1987-02-24|

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CN86105087A|1987-03-04|

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HUT41637A|1987-05-28|

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AU6170086A|1987-02-26|

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IL79765A|1992-02-16|

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PH25861A|1991-12-02|

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HU195425B|1988-05-30|

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EP0211691A2|1987-02-25|

---

ZA866218B|1988-03-30|

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CN1006197B|1989-12-27|

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JPS6251624A|1987-03-06|

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EG17979A|1992-06-30|

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DK396286D0|1986-08-20|

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EP0211691A3|1987-06-03|

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NZ217267A|1989-02-24|

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CA1285874C|1991-07-09|

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AU591516B2|1989-12-07|

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法律状态:
2005-05-10| REG| Reference to a code of a succession state|Ref country code: RU Ref legal event code: MM4A Effective date: 20030820 |

优先权:

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申请号 | 申请日 | 专利标题

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US76860585A| true| 1985-08-23|1985-08-23|

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