Method of obtaining polyester antibiotic

专利摘要:
New polyether antibiotic A80190, its acyl and alkyl ester, acyl ester and urethane derivatives, and salts thereof, are useful antibacterial and anticoccidial agents and increase feed-utilization efficiency in animals. Methods of making A80190 by culture of Actinomodura oligospora NRRL 15877 or NRLL 15878 and synergistic compositions of the A80190 compounds with nicarbazin, 4,4'-dinitrocarbanilide, certain napthalenamine and benzenamine compounds and metichlorpindol are also provided.
公开号:SU1531861A3
申请号:SU853963528
申请日:1985-10-08
公开日:1989-12-23
发明作者:Л.Хамилл Роберт；Че-Фонг Яо Раймонд；Двейн Боек Лаверн
申请人:Эли Лилли Энд Компани (Фирма)；
IPC主号:

专利说明:

Characteristics A80I90. The antibiotic A80190 is attributed to structure I, based on X-ray crystallographic studies.
A80190 in its free acid form) has the following characteristics.
State: white crystals (from acetone - water). M.p. 98-100 or 120- (more often); likely to vary with degree of solvation, pKa 6 6.2 (66% aqueous dimethyl (р щ щ щ). 1
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 (p-1, CHClj).
Mol. Mass 828 (mass spectrometry with desorbed field). UV: no absorption. IR: (CHClI). Shows - Absorption at the following frequencies, cm-: 3019, 2970, 2936, 2827, 1721, 1457, 1402, 1376, 1314, 1163, 1105, 1092, 1083, 1056, 1022, 1006, 989, 980, 945, 934 , 917, 892 and 859.
Calculated,%: C 63.77; H 9.18; About 27.05.
With NTBOi
Found,%: C 63.35; H 9.17; About 27.10.
Solubility. Insoluble in water; soluble in lower alcohols, such as methanol, ketones, such as acetone; esters, such as ethyl acetate
halogenated hydrocarbons such as chloroform and hydrocarbons such as diethylene ether, benzene, toluene, and warm hexane.
A80190 has an acid function capable of forming salts and ester derivatives, and has at least one hydroxyl group that can be etherized or can form ether derivatives or urethane derivatives. Acyl and alkyl esters and apkyl ester derivatives of A801.90, urethane derivatives, pharmaceutically acceptable salts of A80190 and these derivatives are also useful as antibiotics, coxy acids, and as agents that increase the efficiency of animal feed.
According to the purpose of the invention, the description provides a compound (Formulas (I):

Me
Me
J- / - Ko oo- {soV
wherein R is hydrogen or
- CONHR
R.
alkyl, aryl, alkyp-aryl, arylalkyl, haloaryl, nitroaryl, haloarylalkyl, alkoxyaryl, arylok syaryl, aripcycloalkip, aciparyl and cycloalkip, or azip, alkyl-ester or alkyl ester derivatives, or its salts.
A80190 and its derivatives are useful as antibacterial and antiprotozoal agents. They improve the efficiency of feed use in ruminants and act as a means of promoting the growth of animals with one gastric cavity. In addition, they have 1ssecticidal, herbicidal and antiviral activity. In addition, they are useful as ionophores.
Me
Me
/ - K oL- {soV
The term acyl means from C, C, preferably from C to C, an alkanoic acid residue, i.e. rah
formula formulas
before
R
ta
- WITH -
II Oh
in which R is from C to
S.- mil
etc.
alkyl or hydrogen, for example forforcetyl, propionyl, butyryl and
The term cycloalkyl means cyclic hydrocarbon groups containing 3-7 carbon atoms, such as cyclopropyl, cyclobutyl, cyclohexyl, and others, with cyclohexip being preferred. The cycloalkyl group may be substituted with an aryl residue, for example, 2- (phenyl) cyclopropyl.
The term alkoxy means a lower alkyl group from C to C, having a substituted oxygen function in it.
such as methoxy, ethoxy, propoxy, etc.
The term aryl means an aromatic residue obtained by removing a hydrogen atom from an aromatic hydrocarbon, such as, for example, Aenyl, pyridip or furyl, especially phenyl. The aryl residue may be substituted with various groups. The substituent on the phenyl moiety is preferably located at position 4. Examples are A-alkylaryl, for example 4-methylphenyl (4-tolip); 4-haloncephenyl, for example A-chlorophenyl; 4-nitrophe nile; 4-aryloxyaryl, for example 4-phenoxyphenyl; 4-alkoxyphenyl, for example 4-methoxyphenyl; 4- (alkylcarbonyl) -phenyl, for example 4- (methylcarbonyl) -phenyl or 4- (phenylcarbonyl) -phenyl.
The term “alkyl” means C, —C, a straight or branched chain hydrocarbon, preferably a C, —C hydrocarbon, for example methyl, ethyl, propyl, isopropyl, and butyl, etc. The alkyl group may be substituted with an aryl residue, as defined above, to form an arylalkyl residue, such as phenylethyl or 2-phenylethyl, or a halonaryl residue to form a haloarylalkyl residue, such as 4-bromophenylethyl.
Salts of A80I90 and its derivatives are useful for the separation and purification of antibiotics. Particularly useful are pharmaceutically acceptable salts. Such salts should have little or no toxicity in relation to warm-blooded animals. Examples of salts are salts of A8G190 and its derivatives of alkali metals, alkaline earth metals and amines.
The alkali metal and alkaline earth metal salts A80190 include sodium, potassium, lithium, cesium, rubium, barium, calcium and magnesium salts. Amine salts A80190 include ammonium and primary, secondary, and tertiary C, -C alkylammonium and hydroxy alkylpammonium salts. Amine salts include salts formed by the reaction of A80190 with ammonium hydroxide, methylamine, sec-butylamine, isopropylamine, diethylamine, diisopropyl amia, ethanolamine, triethylamine, 3-amino-1-propanol, and the like.
It is widely known in the veterinary pharmaceutical field that form
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An antibiotic is usually not essential for treating an animal with an antibiotic. In most cases, the state of the animal changes the form of the drug, which is different from the form that was introduced. The salt form in which it could be administered is not, therefore, essential. However, the salt form could be chosen for reasons of economy, convenience, and lack of toxicity.
The A80190 antibiotic can be obtained by cultivating A80190, a producing strain of Actinomadura oligospora under deep aerobic conditions in a suitable nutrient medium, until significant antibiotic activity is achieved. The antibiotic is isolated by using various methods of isolation and purification, limestone in the art.
The new Actinomadura oligospora, a microorganism of the present invention that produces the antibiotic A80I90, is isolated from a soil sample from India. Subsequently, a significantly improved version, which produces increased amounts of A80190, was allocated to La Verne D. Doems. The cultures of these two A80190-producing organisms were deposited and became part of the collection of the genus culture in the Northern Regional Research 1 Center (Agricultural Research), from which they are available to the public under the accession numbers NRRL 15877 (parent strain) and NRRL 15878 (strain of the modified individual - variant strain). According to taxonomic studies, two New microorganisms are classified as members of a new species of the genus Actinomadura, for which the name Actinomadura oligospora is suggested.
Characteristics of cultures. Cultivation of the body is usually weak on. a chemically defined medium, but better on a complex organic medium. There are no aerial miels, with the exception of trace amounts on IPS M 4 and sodium butyrate agar. If there are disputes, then their coloring is oyster-white according to the Tresner and Backus systems. The back side is yellowish green to brown. No soluble pigments are priduced, with the exception of producing a very light brown soluble pigment in the case of ISP 2 agar and a dark brown soluble pigment in dextrose-yeast agar. In tab. 1. Represents the characteristics of the NRRL 15878 and .Tasga cultures on various agar media .10
Morphological characteristics. Spore chains are produced scattered on ISP 4 and yatry butyrate agar. The sporophores, containing approximately 15 10 spores per chain, are generally tortuous in a right-hand bending (R-I) configuration. However, hooked sporophores are also observed. Air hyphae (fungus filaments) have a tendency to group together. The spore shape is elongated and varies in size from 0.5 - 0.7 to 0.9 - t ,. The average spore size is 1.1-0. Surface drawing 5
the dispute is smooth.
In tab. Figure 2 shows the carbohydrate use pattern of the NRRL 15878 strain compared to A. Mascah. ISP, (ranked number 9 is used as the main one). The addition of vitamin B or the use of the Luidemann environment contributes to better cultivation, but does not change the use of carbon. Adonite, cellobiose, s glucose and ribose were used. Dubious use is noted in the case of fructose and xylose. Arabinose, cellulose, dextran, galactose, cis-inositol, inulin, lactose, mannitol, mannose, meliosis, melibiosis, raffinose, ramonosis, salicin, sucrose, trehalose and xylitol are not used for cultivation,.
In tab. 3 shows the data on the resistance of the strain NRRL 15878 to various antibiotics at the indicated concentrations and their comparison with A. mash.
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NRRL 15878 cultured at temperatures at concentrations up to 2% NaCl. It produces catalase, phosphatase and urease.
NRRL 15878 degrades casein,. 55 DNA, esculin and gelatin, but does not decompose adenine, calcium acid malic acid, chitin, elastin, guanine, hypopurate, hypoxanthine, keratin, starch,
testosterone, starch, tyrosine or co-antin.
Analysis of cell wall. Hydrolyzed whole cells contain diaminopimelic acid mesoisomer. The sugars present in the whole cell line are the following: glucose, mannose, madurose, and ribose. The cell wall type according to Backer is spike III, and the sugar sample is type B. A qualitative analysis of whole cell methanolysates for mycolic acids gives questionable results. It is doubtful that the culture contains mycolic acids. A type P1 phospholipid sample was detected. Type P1 does not contain nitrogen phospholipids and is characteristic of the genus Actinomadura.
The authenticity of the strain. NRRL 15878 has a type C cell envelope. Type B is a whole cell of a sugar sample and Type P1 is a phospholiptic sample. This homotaxonomic information, along with the general characteristics of the cultures, is consistent with the assignment of the strain to the genus Actinomadura according to Léchevalier. A comparison of its characteristics with those in published descriptions of known species of Actinomadura shows that the culture is similar to A.pelletieri.
Culture resembles Actinomadura pelletieri mainly in the absence of-. effect of at least rare malichea of aerial mycelium. The morphology of the sporophores, when they are produced, is similar to that described in the literature of P3. Two cultures also possess a number of physiological characteristics. However, cultural and physiological differences are sufficient to separate them as a separate species. A.pelletieri causes degradation of elastin, hypoxanthin, and keratin hyrosin, uses trehalose, restores nitrate and cultured at 45 ° C, but NRRL 15878 does not have these characteristics. The latter uses adonite and cellobiose, causes degradation of esculin and DNA, and is resistant to lysozyme, however, A.pelletieri does not have these characteristics. Therefore, a new culture is a species different from A.pelletieri.
Due to the similarity of the A.mash culture, simultaneous laboratory studies of NRRL 15878 and A. Maega are carried out, which have many common properties. Both are not capable of degrading adenine, the calcium salt of malic acid, chitin, elastin, guanine, keratin, starch, testosterone, tyrosine, and xanthine. Neither produces either melanoid pigments. Both cultures cause the degradation of casein, DNA and gelatins, produce catalase and phosphatase, and also synthesize a polyether antibiotic. They have the same tolerance to NaCl, both cultivating well-known physical and chemical mutagens, such as ultraviolet light, X-rays, gamma rays and chemicals, such as N-methyl-N-nitro-N-nitrozogenide. The natural and induced variants, mutants and recombinants of Actioraadura oligospara NRRL 15877, which retain the A80I90 production characteristic, are considered part of this invention.
Extractive medium used for cultivating Acti10 crops
with sodium butyrate, they have the same oligospora, can be any
Naku type of cell membrane. From a number of environments. To save on pro-
A.Masga and NRRL 15878 are bottled to obtain
based on the pattern of carbon utilization, recovery and ease of product release
degradation of esculin and poxanthin, us-some growing media are resistant to antibiotics, temperatures-20 are preferred. So, for example
limit, urease production and nitrate reduction.
A. Masca and NRRL 15878 have many common cultural characteristics, especially the lack of aerial mycelium. However, there are significant differences. The reverse side of the NRRL 15878 is green to yellowish brown, A. Masga is red in many media. This difference appears most clearly on glkzozo-aspartic agar. On this medium, A. masga, in contrast to jNRRL 15878, produces pink aerial growth. These culture comparisons are shown in Table. one .
The morphologists of NRRL I5878 are similar to those of A. masga. Both have poorly developed aerial mycelium. Spore surfaces are smooth. The differences and similarities between NRRL 15878 and A. Masca are outlined in Table. BUT.
In tab. 5 shows the detailed similarities and differences between NRRL 15878 and A. Masg.
These comparisons show that NRRL 15877 and NRRL 15878 cultures differ significantly from other Actinomadura species and represent strains of the new species for which the name Actinornadura oligospora is suggested - a new species. Strain NRRL 15878 is a type strain of A.oligospora. As in the case of other organisms, the characteristics of the A80190 producing cultures of the present invention, Actinomadug oligospora NRRL 15878 are subject to change. Recombinants, mutants, or strain variants can be obtained by processing a variety of
known physical and chemical mutagens, such as ultraviolet light, X-rays, gamma rays, and chemicals, such as N-methyl-N-nitro-N-nitrosoanidine. The natural and induced variants, mutants and recombinants of Actioraadura oligospara NRRL 15877, which retain the A80I90 production characteristic, are considered part of this invention.
Extractive medium used for cultivating Acti crops
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measures, T1, the preferred carbohydrate source for large-scale fermentation (fermentation) is glucose, although it can be used. 5 also ribose, xylose, fructose, galactose, mannose, mannitol, potato dextrin, and the like. Glycerol and lipids support little or no support for the cultivation or production of an antibiotic when used as a main carbon source. In combination with glucose, they increase biomass, but reduce the production of antibiotics.
A preferred nitrogen source is collagen hydrolyzate, although eisin is also useful - hydrolyzed casein, meat peptones, fish meal, liver meal, and the like. Among the nutrient inorganic salts that can be added to the growth medium, soluble salts capable of producing zinc, sodium, magnesium, calcium, ammonium, chloride, carbonate, sulfate, nitrate and the like ions are common.
Valuable trace amounts of elements necessary for the growth and development of the organism should also be included in the growing medium. Such trace amounts of elements are usually found as impurities in other medium substitutes in quantities sufficient to meet the cultured requirements of the organism. Pence education is usually not a problem, but small amounts (i.e.
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0.2 ml / l) of an antifoaming agent, such as polypropylene glycol, can be added to the large scale fermentation fermentation medium if necessary.
For producing significant amounts of antibiotic A80190, deep aerobic fermentation is preferred.
Example 1. Getting A80190. A. Fermentation A80190 in a shake flask. The culture of Actinomadura oligospora NRRL 15878 in a water-free lyophilized pill or in the whole suspension contained in liquid nitrogen is used for sowing on the medium of the following composition, wt%; glucose 1.0; soluble starch 2.0; yeast extract 0.5; enzymatic casein hydrolysis (NZ amine A, Sheffield Kemikal Co., Norwich, New York) 5.0; CaCO 0.1. Deionized water (amount of solution) 1 l. NaOH is added to raise the pH to about 7.2 before sterilization.
Inclined planes and plates are prepared by adding 2.5% agar to the seed medium. Microorganisms on an inclined plane are incubated for about 10-14 days. The culture matured on an inclined plane is scraped with a sterile instrument. About one quarter of the released spores and the culture grown in this way are used to inoculate 50 ml of the seed media of the first stage.
The grafted medium of the first stage is incubated in a 250 ml Erlemeyer flask with a shaker with a circular orbit of two inches (5.08 cm) at a speed of 250 rpm for 48 hours. This medium (0.4 ml) is used for sowing 50 ml of the produced medium having the following composition,%: glucose 3.0; NZ amine A 0.4; collagen hydrolyzate 0.5; H 0.05; CaCOj 0.2. Cold tap water (amount of solution) 1 l. Pre-sterilization is carried out by adjusting the pH to 7.0.
The production medium is incubated in a 250 ml wide-neck Erleimeyer flask with a capacity of 250 ml at 30 for 8–10 days on a shaker with a circular orbit of 5.08 cm at a speed of 250 rpm.
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R. Fermentation A80190 in large volume. To provide a larger volume of seed, 10 ml of the incubated medium of the first stage, prepared as described in section A, are used for grafting 400 ml of nutrient medium of the second stage, having a composition similar to that of the first stage. This vegetative medium. The second stage is incubated in a 2-liter Erleimeier flask with a capacity of 2 liters for 48 hours at 30 ° C on a shaker with a circular orbit of 5.08 dm at a speed of 250 rpm.
The vegetative medium of the second stage thus obtained (800 ml) is used for grafting 100 liters of sterile production medium prepared as described in section A. The production medium is fermented in a 165-liter stirred fermentation tank for 8-10 days at a temperature 30 - 32 ° C. The flow of air (0; 12–0.25 v / v m / min) and the low mixing rate (150–200 rpm) in the stirred vessel maintain the level of dissolved oxygen at 30% air saturation.
Example 2. Branch A80190. The entire fermentation broth from two 100-liter tanks is combined (207 L) and filtered through a filter with Hyflo Supercel. The mycelium sintering mass on the filter is extracted with circulating methanol (40 L) through a filter press. Acetone can also be used as an extracting agent. Ketanol extract, concentrated under vacuum to approx. 15 liters combined with broth filtrate (182 liters). The mixture was adjusted to pH 9 with 1N. sodium hydroxide, the resulting solution was extracted with an equal volume of ethyl acetate. The ethnopacetate extract is concentrated to a volume of about 700 ml. To the concentrated extract was added 1 l of water, the pH was adjusted to 9.0 with sodium hydroxide, the mixture was extracted twice with toluene (1 l), maintaining the pH at 9.0. The toluene extracts were combined and concentrated in vacuo to give an oily residue containing A80190.
The residue was dissolved in toluene (100 ml) and added to a column containing 2 liters of silica gel (Woelm, 70-150 mesh) in toluene. The column was eluted first with toluene (10 l) and then with a mixture of toluene: ethanol (49: 1, 10 l) and (48: 2, 10 l), collecting fractions of 1 l each. The elution was controlled by bio-analysis and those. The fractions containing A80190 are combined and concentrated. The residue was dissolved in dioxane and freeze-dried, yielding 13.6 g of blue A80190.
Example 3. Cleaning A80190.
Crude A80190 (20.4 g)., Obtained from four 100 l tanks, as in Examples 1 and 2, was dissolved in acetonitrile (200 ml). The solution is introduced into a column containing 2 liters of silica gel (toelm, 70-150 mesh) and acetonitrile. The column is followed by aetonitrile (10 L) and eluted sequentially. mixtures of acetonitron acetone (9: 5, 2 l), (9: 1, 1 O l), (4: 1, 10 l) and (7: 3, 10 l), collect fractions of 1 l. Elution is controlled by bioassay using Bacillus subtilis. The fractions containing A80190 are combined and concentrated. The residue is dissolved in dioxane and freeze dried, yielding 15.8 g of purified A80190.
Example 4. A80190 crystallization.
Purified A80190 (28.2 g) was dissolved in acetone (500 ml). Water (500 ml) is added and the pH is adjusted to 5.0 with dilute hydrochloric acid. The resulting solution was allowed to crystallize at room temperature for 20 hours. The crystals are separated by filtration, washed with water and dried in a vacuum oven, yielding 25.9 g of A80190 crystalline (acid form).
Example 5. Getting A80190 sodium salt.
A80190 (acid form, 300 mg) was dissolved in acetone (30 ml), water (10 ml) was added and the pH was adjusted to 10 with NaOH. The solution is concentrated to an oily residue, which is dissolved in dioxane and freeze dried to obtain a white powder (m.p. 145-150). Mol mass of the product by field desorption mass spectrometry (FPD) is 850, G in CHCI3 shows the absorption band maxima at the following frequencies, cm-: 2962, 2934, 2885, 2825, 1719, 1569, 1455, J397, 1377, 1358.
1314.1285, 1243, 1162, 1104, 1074, 1057, 1025, 897, 979, 936, 922, 895 and 847.
, Example 6. Another method of obtaining sodium salt A80190.
A80190 (acid form, 5 g) was dissolved in ethyl acetate (500 mg), water (500 ml) was added. The mixture is adjusted to
Q to pH 10 with 5 NaOH, stirring for 15 minutes, that & support RP. The ethyl acetate phase is separated, washed with water (500 ml) and concentrated under vacuum to dryness. Remainder
5 was dissolved in dioxane (100 ml) and freeze dried, to obtain 4.1 g of sodium salt A80190.
The aqueous washes were left at room temperature overnight, and the resulting crystals were filtered and dried. The crystals are A80190 in acid form (mp. 115-120 ° C).
Example 7: Crystallization of A80190 sodium salt.
5 Pathogenic salt A80190 (1 g) is dissolved in pentane (10 ml) and left to stand at room temperature for three days (the solvent is evaporated to a volume of a mixture of about 5 ml).
0 The crystals that form are filtered and dried under vacuum to obtain 593 mg of crystalline A80190 sodium salt, m.p. 225 - 230 ° C.
Example 8. Preparation of acetylated A80190.
A80I90 (acid form, 200 mg) was dissolved in pyridine (4 ml), acetic anhydrate (4 ml) was added and the mixture was left to stand for 72 hours at
Q room temperature. Add in. do (10 ml), the aqueous solution is extracted with chloroform (50 ml). The chloroform extract is successively taken in 50 ml each with 0.1N. solution
5 HC1, water containing 1Z NaHCO, and water. The chloroform extract is then concentrated under vacuum to a residue, which is dissolved in acetone (100 ml). Acetone solution
0 concentrated under vacuum to remove residual pyrpine and acetic acid. This step is repeated three times, and the residue obtained is dissolved in acetone (50 ml). Water (50 ml) is added and the resulting solution is left to stand at room temperature until crystallization is complete. The crystals are separated by filtration, washed with water and dried in a vacuum.
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kilns at 50 ° C to obtain 192 mg of crystalline acetylated A80, 90 (m.p. 87 -). Mol mass is 870 by mass spectrometry with fast atomic bombardment (MSBAB), IR in ClIClj shows the absorption band maxima at the following frequencies, 3021, 3018, 2935, 2825, 1726, 1454, 1373.1311, 1244, 1162, 1104, 1078, 1058, 1025, 867, 976, 934 and 925.
Example 9. Preparation of propionyl derivative A80190.
A80190 (acid form, 200 mg) was dissolved in pyridine (4 ml), propionic anhydride (4 ml) was added and the mixture was left to stand at room temperature for 72 hours. Water (10 ml) was added and the solution was extracted with chloroform (50 ml). The chloroform extract is washed successively in 50 ml each with 0.1N. HC1 solution, - water containing 1% NaHCG, and water. The chloroform extract is concentrated under vacuum to obtain a residue, which is dissolved in acetone (100 ppm). The acetone solution is concentrated under vacuum to remove residual pyridine and acetic acid. This step is repeated three times. The residue was dissolved in acetone (50 ml), water (50 ml) was added and the mixture was allowed to stand at room temperature for 16-20 hours until the crystallization was complete. The crystals are separated by filtration, washed with water and dried in a vacuum oven at 50 ° C to obtain 170 mg of crystalline propionyl derivative A80190 (mp. 83-8) Mol. the mass is equal to 884 in the MRTD and the MSBAB, IR in CHCl1 shows the maximum of the absorption bands at the following frequencies, cm-: 3018, 2976, 2935, 2827, 1725, 1460, 1376, 1313, 1160, 1105,1082, 1058.1024, 987, 979, 945, 935 and 893. Drimers 10-12. The following ester derivatives A80190 can be obtained by the method of examples 8 and 9: n-heptanoyl derivative A80190, valeryl-derivative A80190, tert-butyryl-derivative A80190.
Example 13. Preparation of 4-bromopheniphetanetane derivative A80190.
A801 90 (acid dbopMa, 7 g) is dissolved in. benzene (100 ml) and 4-bromophenyl isioate (3 g) in benzene (100 ml) were added with stirring. - The mixture is stirred at room temperature.

. . . ,
ten
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20
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Perform for 168 hours. The reaction is monitored daily by silica gel TLC using acetonitrile: acetone (1: 1) as a developing solvent, and detected by means of a dispersant — a mixture of vanillin and,. The reaction is completed in 168 hours. The resulting precipitate is separated by filtration, and the filtrate, containing the derivative and some unreacted A80I90, is concentrated and lyophilized, yielding 7.5 g of the reaction product.
This product was dissolved in acetonitrile (50 ml) and introduced into a column containing 800 ml of silica gel (Vfoelm, 70-150 mesh) in acetone trillet. The column was developed with acetonitrile, a first fraction with a volume of 500 ml, five subsequent fractions of 100 ml each, and then five fractions of 500 ml each. Elution is monitored by TLC on silica gel. Crystals form in the fourth fraction after standing at room temperature overnight. The crystals were collected by filtration and dried under vacuum to obtain 639 mg of 4-bromophenylurethane derivative A80I90.
The filtrate is dried. The residue was dissolved in dioxane (and lyophilized to give additional product (477 mg). The fifth in tenth fractions were combined and concentrated to dryness. The residue was dissolved in acetonitrile (50 ml) and lyophilized, yielding an additional 2.3 g amorphous 4-bromophenylurethane derivative A80190.This compound has the following characteristics: mp. 142 - 145 ° C, o (V +4 C (s 10, CHClj). Mol. mass 1025 (USBAB).
Examples 14-15. The following urethane derivatives of A80190 are prepared using the procedure of Example 13: 4-chlorophenylurethane-derived A80190 and 4-nitropheniphetane-derivative A80190.
Examples 16-23. The following urethane derivatives can be obtained using the procedure of Example 13: phenylurethane-derivative 180190; 4-me 55 tsh1fensh1uretan-derivative A80190; 4-iodophenylurethane-derivative A80J90; 4-fluorophenylurethane-derivative A80190; cyclohexpentane derivative A80190; 2-phenipztiluretan-derivative A80190;
thirty
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171531861
cyclopropylurethane-proeevodnoe
g for but but
A80190; 4-phenoxyphenylurethane derivative A80190.
Example 24. Production of methyl ester manufactured by A80190.
A80190, in acid form, is dissolved in methanol and water (1/2 volume) is added. The solution is left until an ether derivative is formed. The solution is evaporated under vacuum. The product of the reaction is rutot chromatography using, for example, Silica gel and obtaining the methyl ester derivative A80190.
Examples 25-27. Using a procedure similar to that of Example 24, and the corresponding alcohol or thiol, the following ether derivatives of A80190 are obtained: i-propyl ether derivative A80190; methyl thioester derivative of A80190; and -butylether derivative A80190.
Example 28. Chromatographic identification A80190.
I. those on silica gel. System: acetonitrile - acetone (1; 1). Rf 0.59 Detection: Bacillus subtilis. Spraying vanilla mixture -.
Sows are fattened at rates of 1.0–10 g of A80190 per 100 pounds (45.36 kg) of feed. Medical feed predII. HPLC (AO liquid chromatography is left with water. Usually, sowing capacity), Adsor is about 6-8 pounds consumed.
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bent: (uBondapak C, j (4xZOO-mm column). Solvent system: acetonitripe - tetrahydrofuran - water (6: 1: 3), containing 1% Nepo. Adjust pH to 3.0 using NH40H. Detection: refractometer, flow rate 3.0 ml / min, retention time 9.7 minutes.
Example 29. A80190 receive according to the method of example 1, but using Actinomadura oligosporus NRRL 15877 culture.
Example 30 A80190 modified chicken diet for coccidiosis control.
In tab. 6 shows a balanced, high-calorie diet adapted to fattening chickens there.
for quick weight gain.
""but-
 These substances are mixed according to standard methods.
| Shivan feed. Chickens fed with such a diet with water are resistant
to coccidiosis.
Example 3l. The diet of meat cattle, improved additive A80190.
In tab. 7 shows a balanced, highly fragmented diet for meat cattle.
(2.72 - 3.63 kg) diet per day.
Example 33. A80190 - recipe for piglets.
A801 90 is dissolved in a small amount of ethanol. This ethanol solution is suspended in polyethylene glycol 200. The suspension concentrates in such a way that each unit dose has a volume of about 0.5-2 ml. Such sus: pensy give young pigs m at a nor-, max 0.5-50 ng per pound three times a day by forced fattening.
Example 34. Improved ration (premix) for the control of porcine dysentery is given in table. 9.
This premix is added to the conventional pig ration using standard feed mixing techniques, giving a final level of active compound of 100 g / t.
The synergistic effect of A80190 with nicarbazine was carried out as follows: one week old broiler chickens were spread into cages in five birds and fed, with therapeutic and control diets, for one day, and then ingested with coccidi oocytes, causing coccidi 55
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Mixed feed is compressed into feed pellets. With a daily average daily intake rate of 15 Pounds per animal, approximately 300 mg of A80190 (Na-salt) per animal per day is added to the feed.
Example 32. Diet for pigs, improved by addition of A80190.
In tab. 8 shows a balanced diet for farrowing pigs.
For 200 pounds (90.72 kg) of this diet, the premix is prepared by adding A80190 (10 g) to a small amount of solvent-extracted soy food. This premix is then added to 200 pounds of the described porcine diet, mixed by standard methods. This medical food provides the level of 100 g A80190 per 1 ton of the basic ration. The therapeutic food feeds the sows for one day, preferably 7-10 days before the birth, the introduction of this feed after farrowing is also desirable.
Sows are fattened at rates of 1.0–10 g of A80190 per 100 pounds (45.36 kg) of feed. Medical food pre
put with water. Usually sow-and consume about 6-8 pounds.
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(2.72 - 3.63 kg) diet per day.
Example 33. A80190 - recipe for piglets.
A801 90 is dissolved in a small amount of ethanol. This ethanol solution is suspended in polyethylene glycol 200. The suspension concentrates in such a way that each unit dose has a volume of about 0.5-2 ml. Such sus: pensy give young pigs m at a nor-, max 0.5-50 ng per pound three times a day by forced fattening.
Example 34. Improved ration (premix) for the control of porcine dysentery is given in table. 9.
This premix is added to the conventional pig ration using standard feed mixing techniques, giving a final level of active compound of 100 g / t.
The synergistic effect of A80190 with nicarbazine was carried out as follows: one week old broiler chickens were spread into cages in five birds and fed, with therapeutic and control diets, for one day, and then ingested with coccidi oocytes.

ÜMe Me,
Lh / H
 ofto nj
wherein R is hydrogen or
-CONHR; R is alkyl, aryl, alkyl-arip,
arylalkyl, haloaryl, nitroaryl, haloarylalkyl, alkoxyaryl, aryloxyaryl, arylCycloalkyl, acylaryl, and picloalkyl, or acyl, alkyl ester or alkyl ether derivative.
No. 2 Height: Good (wet surface)
OS: 61. Green-yellow. Brown
VM: Missing RP: Very light brown No. 3 Height: Poor
OS: 93. Yellowish gray
VM: Footprints: black and white
Several scattered individual lumps of RP: None
# 4 Height: Poor
25
thirty
OR
or its salt, including the cultivation of a strain of the microorganism Actino-madura oligosporus NRRL 13877 or
 a strain of the microorganism A.oligosporus NRRL 15878 on a nutrient medium containing digestible sources of carbon, nitrogen and inorganic salts under conditions of deep-seated aerobic fermentation at 30 — followed by release of the antibiotic.
Table 1
Excess
39. Green. Red
Oyster
43. Red Brown
56. Dark brown
Otsuottsbet
Very light brown
Mediocre 5. Milky. Pink None
Missing
Mediocre to
bad


RP: Growth:
Ls-os; VC:
RP: Growth:
Height: OS:
VM:
RP:
Height: a
OS:
VC:
RP:
Height: OS:
None Good (wet surface) 90. Greenish yellow. 26
Yellow None
Missing
Excessive (wet surface) 79.1. Zel.-yellow. Yellow. Brown39,
Missing
Missing
Excess (wet and
surface)
81. Dark green-yellow brown59.
Missing
Dark brown
Good
93. Yellow gray78.
Red brown No Good
S Yellow Pink
Mediocre. White - 7 sa I Yellow-pink None
Good
Zel.-yellow. Red. Oyster Traces. 2 Smooth (only edges) None Excess (wet surface)
Dark brown
Minor trace
Gray
Dark Brownbread
Mediocre
Dark yellow
The black
VN:
RP:
Height: OS: VM: RP:
Mediating
e. White
Missing
Mediocre
93. Yellow gray
Missing
Missing
Adonite l-arab
Zell
Deke D-Fruck D-Gala D-Gluck-in
Inul D-Lactose
Cannit D-Mannose D-Melesit
Note - stable (no zones;
angiography); - - sensitive (zones of inhibition).
None None Mediocre S Yellow-pink None None
± - doubtful if35 uses {; ;
NP - not tested.
Table 3
Similarities
Morphologists
Physiological properties
Polyester Synthesis Deficit of Air Hyphae
Education vozdushnyh Fov Painting using Lerod
Catalanza degradation of hellin
calcium salt of blast acid casein chitin DNA of elastin exulin gelatin guinine hypoxanthine keratin starch testosterone tyrosine xanthine
Liquid cultivation
environment
Na Butter Cultivation
The production of H S # Llanoid pigments Tolerance to NaCl,
T
Table 4 Differences
Antibiotic resistance
Painting use by the kind of angle
Culture characteristics
Esculin degradation, hypoxantine degradation, nitrate reduction
The shape and size of the dispute Temperature limit Urease production
Table 5
Seldom
(see tab. 2)
+ +
“+
Red orange
Reduction of Phosphatase nitrate Synthesis of polyester Athybiotic resistance pattern
Resistance to lysozyme Coloring the reverse side
Soluble pigment Pepi spores Spore surface Temperatures limit, ° C Urease production Spore form Spore sizes
Soloya soybean flour, extracted with thin skin, extracted with solvent, finely milled, 50% protein
Animal fat (rendered beef fat)
Dried fishmeal with soluble substances (60% protein)
Corn bard with soluble substances Dicalcium phosphate, feed grade
Calcium carbonate Vitamin premix (representing vitamins A, D, E, K and B, choline, niacin, pant genic acid, riboflavin, biotin with glucose as a filler)
Traces of mineral premix (representing, KS, FeSO, CaCOj) 2-amino-4-hydroxy-butyl acid (methionine oxy-analog) A80190 (Na-salt)



f +
four
(cm.
.tables 3) Do not spend korichn. -
-or nzh. Brown
R - And Smooth 15-37
Obligate Oval
From 0.5-0.7 to 0.8-1.0 to
0.9-1, 2-2, Org,
31.09 282.05 (621.8) 6.5 58.97 (130)
0.5
4.54 (10)

1.81 (4)
0.91 (2) 0.09 (0.2)
29
Thinly ground corn Corn cob Corn cob Dehydrated alfalfa flour, 17% protein
Containing per pound (453.6 g): 2,000,000 IU. Vitamin A, 227.200 M.E. Vitamin Dj. and 385.7 g of soybean feed with the addition of 1% oil.
 Dry corn bard with soluble substances containing 20,000 M.U. 0-alpha tocopheripacetate per pound.
Yellow maize hammer Soya oil on flour with peeled, solvent extracted
Dried beet pulp Dicalcium phosphate I Calcium carbonate Pork vitamin premix Salt (NaCl) Choline hlorchts, 25% Traces of mineral premix
1531861 0
Table 7
67u8 615.08 (1356) 1090.72 (200)
5A5.36 (100)
T a b l and c a 8
65.10 590.59 (1302)
167.83 (370)
90.72 (200)
26.31 (58)
10.89 (24) 9.98 (22) 4.99 (II) 3.175 (7) 1.36 (3)
31
Part of
Vitamin A - Premix "Methionine Oxy-Analogue
 1 kg of pre-mix contains the following components: 77.161 E. The US State Pharmacopoeia (USP) of vitamin Dj; 2,205 M.E. vitamin E; 441 mg riboflavin; 1,620 mg pantogenic acid; 2.205 mg niacin; 4.4 mg of vitamin B; 441 mg of vitamin K; 19.180 mg of choline; 110 mg of 4-uric acid; 165 mg pyridoxine; PO mg of thiamine and 22 mg of biotin.
  kg premix a contains the following components: 50 g of manganese in the form of manganese sulphate; 100 g of zinc in the form of zinc carbonate; 50 g of iron in the form of sulfate, - ferrous oxide salts; 5 g of copper in the form of copper oxide; 1.5 g of iodine as potassium iodide and 150 g maximum and 130 g minimum of calcium as calcium carbonate.
 1 kg of premix contains 6.6138 00 USP E. vitamin A.
Part of
Active connection
Calcium silicate
Calcium Carbonate
(oyster flour
shells)

9.13 9.13 8.88 8.90 8.66 0.08
8.63 5.13 1, 63 0.56 0.0
153186132
Continuation of table.8
Content
%
kg / t (lb / t)
0.10 0.05
0.9 (2) 0.54 (1)
Table 9
Content, g / kg
Tabli yes 10

3.50 0.13 0.0
1.19 0.0
0.0
33
1531861
- 34 Table II

权利要求:
Claims (3)
[1]
Yu claims
A method of obtaining a polyester antibiotic A80190 of the formula in which R is hydrogen or —CONHR _f ;
R ₍ - alkyl, aryl, alkyl-aryl, • arylalkyl, haloaryl, nitroaryl, haloarylalkyl, alkoxyaryl, aryloxyaryl, arylcycloalkyl, acylaryl and picloalkip, or acyl, alkyl ester or alkyl ester derivative,
25 or its salt, including cultivating a strain of the microorganism Actinomadura oligosporus NRRL 15877 or a strain of the microorganism A.oligosporus NRRL 15878 on a nutrient medium that maintains digestible sources of carbon, nitrogen and inorganic salts under conditions of deep aerobic fermentation at 30 - 32 ° С subsequent isolation of the antibiotic.
Table 1
---------, Agar medium ------_------------------------NRRL 15878 A. masga 1 2 3
ISP No. 2 Height: Good (wet Excess top) 39. Zelgeltoy. Red OysterOS: 61. Green-yellow. To- 43. Reddish brown brown 56. ’Dark BrownVM: Is absent OtsuNoRP: Very light brown Very light brown high ISP No. 3 Height: Bad MediocreOS: 93. Yellowish Gray 5. Dairy. PinkVM: Traces: black and white Is absent Somewhat scattered separate lumps RP: Is absent Is absent ISP No. 4 Height: Bad From mediocre to
bad
21 1531861 22Continuation of the table. ; g1 3 OS: 93. Yellowish Gray 5. Dairy. Pink VM: Traces: black and white A few scattered individual lumps Is absent RP: Is absent Is absent ISP K * 5 Height: Bad Good OS: 93. Yellowish Gray 48. Oyster VM: Is absent Is absent RP: Is absent Is absent ISP Ν ’7 Height: Bad (wet surface) Mediocre OS: 79.1. Green-yellow.Yellow. Brown 48. V. Oyster VM: Is absent Is absent RP: Light brown Is absent Capek Height: Mediocre Mediocre OS: 93. Yellow Gray 93. Yellow Gray VM: Is absent Is absent RP: Is absent Is absent Emerson Height: Good (edges flaky on the back) Good OS: 77. Dairy. Yellow - brown 267. The black VM: Is absent Traces: 5 fe 1. Green-yellow. Red brown RP: Is absent Is absent Height: Good (wet surface) 'Good Glucose* LS-OS: 90. Greenish yellow. 26. S Yellow Pink + paragin VM: Yellow Missing Mediocre. White - 7 sa I Yellow Pink RP: Is absent Is absent Tomato Height:paste Oatmeal Height: Excessive (wet) Good flour OS: surface)79.1. Green-yellow. Yellow. Brown 39. Green-Yellow. Red. Oyster VM: Is absent Traces. 2 White (edges only) RP: Is absent Is absent Yeast* Height: Excessive (wet and, -. Excessive (wet dextrose OS: surface)81. Dark green-yellow - surface)brown 59. Dark brown VM: Is absent Slight Traces of Gray RP: Dark brown Dark brown Butyrate Height: Good Mediocre sodium OS: 93. Yellow Gray 78. Dark Yellow The black
23 1 531861 24Continuation of table 1 11 3 VM: Medium B. White Is absent RP: Is absent Is absent Calcium Height: Mediocre Mediocre apple salt, - OS: 93. Yellow Gray 26 . S Yellow Pink night VM: Is absent Is absent you are sour RP: Is absent Is absent Prime h and u e. OS - the reverse side; VM - aerial mycelium
RP is a soluble pigment.
Continuation of the table. 2
1 ... ΞΖΤ 2_t --- D-MelibiosisRaffinose - - L-Ramnose - - Ribose +. t Salicin - Sucrose - Trehalose - + Xylitol - NP D-xylose ±
T a b l i
2
Carbon Source NRRL 15878 A. masga 1 2 3 Control -. - Adocyte + L-Arabcnose ““ Cellobiosis + - Pulp - NN Dextran - NP D-Fructose t D-galactose - + D-Glucose + + Peace Inositol - t Inulin - NP D-Lactose - - Mannitol - - D-mannose - - D-melesitosis
Note. + - used;
- - not used; ± - doubtful if used; NP - not tested.
Table 3 '
Concentration NRRL .15878 ^ A.Massga
Antibiotic
Bacitracin 1 Ored. + - Cephalotin 30 μg + + Gentamicin 10μ g - - Lincomycin 2 μg + + Neomycin 30 μg - - Oleandomycin 15 μg - Penicillin C Yu units + + Rifampin 5μ g + + Streptomycin Yur g - + Tetracycline 30 μg - - Tebramycin 1 0 μ g - - Vancomycin 30 μg - -
Note * - stable (no activation zones); - - sensitive (zone of inhibition).
I
Table 4 Similarities j Differences Morphology Antibiotic Resistance civility Physiologists physical properties Use picture corner of kind Synthesis of Cultural characteristics diester rice teak and Deficit stuffy gifs Esculin degradation
hypoxanthine degradation, nitrate reduction
Shape and size of spores Temperature limit Urease production
Table 5
Test NRRL 15878 A. masga 12 3
Airborne Hygiene fow Seldom Seldom The picture of the use of Leroda Different (see table 2) Catalase degradation: adé nina - - apple calcium salt acid * - casein + + chitin- DNA + + elastin . - · “ exulin + - gelatins + + guanine - hypoxanthine - ♦ keratin - starchtestosterone · tyrosine· “ xanthine - - Cultivation in liquid environment Colorless Red orange Cultivation on buty- rate Na + + Producing H ^ S - Melanoid pigments - - Tolerance to NaCl,% 2 2
Continuation of Table 5
.1 g 3 Nitrate reduction - + Phosphatase + + Polyester Synthesis + + tibiotics Different (see table 3) Lysozyme resistance + Did not Back side coloring Green .- * Brown - " - * brown - * orang. Soluble pigment Brown Brown Chains of dispute R - And R - And Surface spores Smooth Smooth Temperature limit ° C 15 - 42 15-37 Urease Production - " - Dispute form Oblong Oval Dispute sizes 0.5-0.7 to Sn 0.8-1.0 to 0, 9-1, 3 μ m 1, 2-2.0 μτη
Table 6
Content
Component
X kg (lbs)
Ground yellow corn fifty 453.6 (1000) Solvent-extracted soy flour, finely ground, 50X protein 31.09 282.05 (621.8) Animal fat (melted beef tallow) 6.5 58.97 (130) Dried fish meal with soluble substances (60Ζ protein) 5,0 45.36 (100) Soluble Corn Bard 4.0 36.29 (80) Dicalcium phosphate, feed grade 1.8 16.33 (36) Calcium carbonate 0.9 7.26 (16) Vitamin premix (representing vitamins A, D, E, K and B ₍₁ , choline, niacin, pantothenic acid, riboflavin, biotin with glucose as a filler) 0.5 4,54 (YU) Traces of mineral premix (representing MnS0 ₄ ZnO, KS, FeSO ₄ , CaCOj) 0.2 1, 81 (4) 2-amino-4-hydroxybutyric acid (hydroxy analogue of methionine) 0.1 0.91 (2) A80I90 (Na salt) 0.01 0.09 (0.2)
Table 7
Content
Component (Lunts)
Finely ground corn 67, 8 615.08 (1356) Ground Corn Cob 10 90.72 (200) Dehydrated alfalfa mu. ka, 172 squirrels 5 45.36 (100) Soy flour with skin extract extracted vitorite, 502 protein 9,9956 90.68 (199,912) Sugarcane molasses 5 45.36 (100) Urea 0.6 5.44 (12.0) A 80190 (Na salt) 0.0044 0.04 (0,088) Dicalcium phosphate, feed grade 0.5 4,54 (10.0) Calcium carbonate 0.5 4,54 (10.0) Sodium Chloride oh s 2.72 (6.0) Traces of Mineral Premix 0,03 0.27 (0.6) Vitamins A and 0 _g premix * 0,07 0.635 (1.4) Vitamin E premix * * 0.05 0.454 (1,0) Calcium Propionate 0.15 1.36 (3.0) * Containing per pound (453.6 g): 2,000,000 M.E. 227.200 M.E. vitamin and 385.7 g soy . vitamin feed s to
12 oil added.
** Dry corn bard with soluble substances containing 20,000 M.E. O-alpha tocopheryl acetate per pound.
Component
Content
[2]
2 j kg / t (lb / t)
Ground yellow corn 65.10 590.59 (1 302) Soybean Oil Flour Removed skin-extracted solvent > 8.50 167.83 (370) Dried Beet Pulp 10.00 90.72 (200) Dicalcium phosphate 2.90 26.31 (58) Calcium carbonate 1.20 10.89 (24) Pork Vitamin Premix * 1.10 9.98 (22) Salt (NaCl) 0.55 4.99 (AND) Choline Chloride, 252 0.35 3, 175 (7) Traces of mineral premix ** 0.15 1.36 (3)
[3]
3i
153186]
Continuation of Table 8
ComponentContent kg / t (lb / t) Vitamin A - Premix ♦ ** Oxy-analogue of methionine0.100.05 0.91 (2)0.454 (1)
* 1 kg of premix contains the following components: 77.161 E. US State Pharmacopoeia (USP) Vitamin D ₂ ; 2.205 M.E, vitamin E; 441 mg riboflavin; 1.620 mg of pantogenic acid;
2.205 mg niacin; 4.4 mg of vitamin B ₁ ; 441 mg of vitamin K; 19.180 mg of choline; 110 mg of folic acid; 165 mg pyridoxine; 110 mg of thiamine and 22 mg of biotin.
** I kg of premix contains the following components: 50 g of manganese in the form of manganese sulfate;
100 g of zinc in the form of zinc carbonate; 50 g of iron in the form of sulfate salt of ferrous oxide; 5 g of copper in the form of copper oxide; 1.5 g of iodine in the form of potassium iodide and I50 g maximum and 130 g minimum calcium in the form of calcium carbonate.
*** 1 kg of premix contains 6.6138 00 USP E. Vitamin ι per A.
Table 9
Component Content, g / kg Active connection 150.0 Calcium silicate 20,0 Calcium carbonate (oyster flour shell) 830.0
Table
Nicarbazine (ppm) 0 4 8 12 16 '0 9.13 8.63 5.94 3,50 1.19 20 9.13 5.13 0.19 0.13 0,0 thirty 8.88 1,63 0.50 0,0 40 8.90 0.56 0,0fifty 8.66 0,0 125 0.08
Table 11
------- ---- η Nicarbazine (ppm) ----- ---,0 4 ----------,8 -12 16 20
0 3.44 2.63 1.75 1.19 0.19 20 3.19 2,38 0.31 0.56 0.06 thirty 3,50 0.92 0.19 0,0 40 3.17 1.00 0.58fifty 3.19 0.54 125 0.33
Table 12
Nicarbaein (ppm) 0 4 8 12 16 20 0 147 236 265 251 272 261 20 159 268 284 266 279 thirty 174 279 258 28340 211 258 289 fifty 222 276 125 279

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引用文献:

---

公开号 | 申请日 | 公开日 | 申请人 | 专利标题

---

---

GB2113671A|1982-01-19|1983-08-10|Pfizer Ltd|Polycyclic ether antibiotics|US4824829A|1984-08-15|1989-04-25|American Cyanamid Company|Non-dusting antibiotic, anticoccidial premix compositions and a process for their manufacture|

---

WO1989006963A1|1988-02-08|1989-08-10|Pfizer Inc.|Acidic polycyclic ether antibiotic having anticoccidial and growth promotant activity|

---

RU1825377C|1988-05-02|1993-06-30|Эли Лилли Энд Компани|Method for producing a 82810 antibiotic or its derivatives and "actinomadura fibrosa" strain - a 82810 antibiotic producer|

---

US5215981A|1988-12-29|1993-06-01|Hokko Chemical Industry Co., Ltd.|Polyether antibiotic mi215-nf3 substance, production process thereof, and agent for control of chicken coccidiosis|

---

US5217993A|1992-07-20|1993-06-08|Eli Lilly And Company|Monensin derivatives|

法律状态:

优先权:

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申请号 | 申请日 | 专利标题

---

US06/658,977|US4683204A|1984-10-09|1984-10-09|Process for producing antibiotic A80190|

---

US06/658,976|US4582822A|1984-10-09|1984-10-09|Antibiotic A80190, pharmaceutical compositions containing same and method of use|

---

CA000492361A|CA1320464C|1984-10-09|1985-10-07|Antibiotic a80190|

---