前苏联专利SU1528329A3 Method of obtaining peroral composition for prophylaxis of poultry coccidiosis

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专利摘要:
An oral vaccine against parasitic protozoa, such as coccidia, comprises live encysted protozoa embedded in a firm gel matrix from which the protozoa are released following ingestion by a host. The gel matrix helps to maintain the essential viability of the protozoa without seriously hindering the natural mechanism by which they infect their host. Preferably the gel matrix is based on alginate. Alginate beadlets containing viable sporulated coccidia oocysts, for administration to poultry, are a preferred feature.
公开号:SU1528329A3
申请号:SU853874750
申请日:1985-04-01
公开日:1989-12-07
发明作者:Джеймс Дэвис Поль；Харрис Петер
申请人:Юнилевер Н.В. (Фирма)；
IPC主号:

专利说明:

The invention relates to biotechnology, and more specifically to the creation of vaccine preparations for the prevention of diseases of farm animals.
The aim of the invention is to repeat the immaturity of the composition.
Example 1. Sodium alginate (llanucol LB) is used as a gel base, characterized by a high ratio of acid residues of mannuric, guluronic acids and low molecular weight (Manucol is the trade name of the company Kelko / Alh International).
95 ml of an aqueous solution containing 11% by weight of itanucol LB are mixed with 5 ml of an aqueous suspension of viable sporulated coccidian oocysts (Eimeria
tenella) containing about 600,000 oocysts. This mixture is poured dropwise from a height of 76.2 mm into a 5% water-No. 1 solution of calcium chloride dihydrate. Get the beads curdled gel with a diameter of 5 mm and a weight of 40 mg. 2 500 beads are obtained within 2 minutes.
The beads are left in the coagulation bath for 15 minutes, separated from the coagulation agent, and then soaked overnight with a tap water to remove excess calcium chloride. Then the beads are dried on absorbent paper at ambient temperature at a final moisture content of 15-16%.

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Example 2. Sodium alginate is used as a gel material, and glycine is used as a moisturizer.
45 ml of an aqueous solution containing 11 wt.% Manucol LB (sodium alginate) are mixed with 5 ml of a suspension of xoctidia oocysts (as in Example 1) and 50 ml of glycerol. Beads are obtained from the resulting mixture as in Example 1, except that excess calcium chloride is removed from the gelatinized beads by soaking.
age, and the second group gets a normal, non-oocysts diet. Birds are kept behind the wire, so the amount (the amount of oocycid taken per day can be regulated. The room in which the birds are kept is carefully pre-treated to ensure the absence of the remaining oocysts.
Oocysts are obtained by infecting four weeks old broiler chickens with an oral dose of 50,000 oocysts to each chicken. Their litter is collected
not overnight in a water / glycene mixture on the sixth, seventh and eighth day
50/50, the final moisture content of dry beads is 15-16 wt.%.
The viability of oocysts of coccidia in alginate beads, obtained in accordance with examples 1 and 2, is determined as follows.
After being kept in a dehydrated state for several days, a sample of alginate beads is mixed with 500 grams of regular poultry feed in such a way that each 500 gram sample contains approximately 2500 beads. Each feed sample is given to two 30-day tsips there on demand for two days. With the safe viability of the oocysts in all birds, the clinical symptom of coccidiosis with blood in the litter is observed on the fifth and sixth day after ingestion of food containing oocytes.
The experiment shows that a large number of coccidia oocysts remain in a viable form, despite the treatment with which they are subjected, and they could satisfactorily exist in the intestine, despite being embedded in a solid alginate gel matrix.
Example 6 An experiment was conducted to confirm that a very small amount of virulent sporulated oocysts added to the poultry feed composition according to the proposed method can survive and cause controlled subclinical infection when administered to susceptible birds. Immunity created during such treatment for 27 days.
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after infection, mix with approximately double the volume of a 2.5% aqueous solution of potassium dichromate and then homogenize to form a homogeneous suspension. The suspension is filtered through a nylon sieve, and the OKOCCY are separated from the filtrate by saline flotation as follows. First, the oocysts are seditated by centrifugation at a rate of 1 OOO g for 15 minutes, and the precipitate is resuspended in a saturated aqueous solution of sodium chloride. The resulting suspension is centrifuged at a rate of 500 g for 10 minutes and the remaining suspension of oocysts is diluted with 10 volumes of distilled water. As a result of centrifuging at a rate of 1 for 15 minutes, granules are formed from the CYOCYSTE. The entire procedure of salt flotation is repeated and, finally, the obtained oocysts granules are placed in a 2.5% aqueous solution of potassium dichromate. Extracted NUZYSTYs are sporulated by continuous aeration of the suspensions for 4 days at 28 ° C. These NUZYSTYA are stored at 4 ° C in a phosphate buffer solution as a suspension of the strain
Bisser is prepared according to the method described in Example 1, with the exception that the total number of sporulated coccidia oocysts 30,000,240 beads is added to 3 kg of chicken feed and given to day-old chickens there for 3 weeks. The number of oocysts excreted with feces from each group is monitored.
It is established that oocysts survive
conducted by administering 50,000 oocysts, - - in the diet during the experiment period. Two groups of 12 birds each are taken. The first group receives an oocyst-containing diet, starting with a one-day
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five
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0
after infection, mix with approximately double the volume of a 2.5% aqueous solution of potassium dichromate and then homogenize to form a homogeneous suspension. The suspension is filtered through a nylon sieve, and the OKOCCY are separated from the filtrate by saline flotation as follows. First, the oocysts are seditated by centrifugation at a rate of 1 OOO g for 15 minutes, and the precipitate is resuspended in a saturated aqueous solution of sodium chloride. The resulting suspension is centrifuged at a rate of 500 g for 10 minutes and the remaining suspension of oocysts is diluted with 10 volumes of distilled water. As a result of centrifuging at a rate of 1 for 15 minutes, granules are formed from the CYOCYSTE. The entire procedure of salt flotation is repeated and, finally, the obtained oocysts granules are placed in a 2.5% aqueous solution of potassium dichromate. Extracted NUZYSTYs are sporulated by continuous aeration of the suspensions for 4 days at 28 ° C. These NUZYSTYA are stored at 4 ° C in a phosphate buffer solution as a suspension of the strain
Bisser is prepared according to the method described in Example 1, with the exception that the total number of sporulated coccidia oocysts 30,000,240 beads is added to 3 kg of chicken feed and given to day-old chickens there for 3 weeks. The number of oocysts excreted with feces from each group is monitored.
It is established that oocysts survive
that Substantial immunity begins to develop soon after the chicks have reached two weeks of age.
Formula
3 o
bratis
as there is a sharp increase in oocysts, which is reduced by 21 days,
Obtsists, called immunity, do not have any pathogenic effect. During the entire period of immunization, no blood is observed in the litter and, in addition, the average weight gain of the control group and / group that is immune is 11) is actually the same until the day of the control infection with their chain of test,
A control infection of 50,000 oocysts on the 27th stage causes serious diseases containing 120,000 cysts in 1 ml, before the control group leaves, that can have a final concentration of 6,000 cysts per day.
ten
A method of preparing an oral position for the prevention of coccidiosis in poultry, comprising mixing a suspension of sporulated cyst cysts with filler, which also means that, in order to increase the immunogenicity of the composition, algi sodium, an 11% solution of which is mixed with a suspension of sporulated cyst coccidia Eimeria tenella,
Judging by the blood content in the litter and the abundant release of oocysts. The immunized group is clearly not exposed to infection, but mortality is not observed in both groups.
Examples 4-7. Use of fillers for the production of alginate beads.
Beads are obtained according to the method described in example 1, using the solution indicated in the table.
In Examples 2-7, after coagulation and drying, as in Example 1, a strong bisor is obtained.
Maliso LM is an alginate with a high ratio of mannuranic acid residues; guluronic acid and high molecular weight. When a filler is used, high molecular weight alginate is preferred in order to obtain a gel of the required strength.
rmul
6
3 o
bratis
containing 120,000 cysts in 1 ml, to a final concentration of 6000 cysts in
A method for preparing an oral composition for the prevention of coccidiosis in poultry, including mixing a suspension of sporulated coccidia cysts with a filler, about one hundred percent, and so that, in order to increase the immunogenicity of the composition, algi-: sodium, whose 11% solution is mixed with a suspension of Eimeria tenella sporulated coccidia cysts,
20
ml, after which the mixture is poured dropwise from a height of 76.2 mm into a 5% aqueous solution of calcium chloride dihydrate obtained at this bead is kept in this solution for 15 minutes, after which they are removed, treated with running water and dried to a residual moisture content of 15-16%.
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thirty
five
6 7
110% commercial white wheat flour
0,2510% | commercial white wheat flour
110% kaolin
0,2510% kaolin

权利要求:
Claims (1)
[1]
Claim
A method of obtaining an oral composition for the prevention of coccidiosis in poultry, comprising mixing a suspension of sporulated coccidia cysts with an excipient, in that, in order to increase the immunogenicity of the composition, alginate is used as an excipient: sodium, 1 1% solution of which is mixed with a suspension of sporulated coccidia cysts Eimeria tenella, containing 120,000 cysts in 1 ml, to a final concentration of 6000 cysts in 1 ml, after which the mixture is poured dropwise from a height of 76.2 mm in 5% aqueous solution of chl dihydrate chloride ..kaltsiya obtained with beads maintained in the solution for 15 minutes, after which they were removed, Ind> 1vayut running water, and dried to a residual humidity of 15-16%.
Example
Alginate Manucol LM,%
Filler
10% commercial white ginat with a high ratio of mannuranic acid residues: guluronic acid and high molecular weight. When using a filler, high molecular weight alginate is preferred to provide a gel of the required strength.
5 0.25 wheat flour 10%, commercial white wheat flour
1 10% kaolin
0.25 10% kaolin

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同族专利:

公开号 | 公开日

AU3211584A|1985-03-12|

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JPS60501955A|1985-11-14|

DK153685A|1985-04-03|

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US4148689A|1976-05-14|1979-04-10|Sanraku-Ocean Co., Ltd.|Immobilization of microorganisms in a hydrophilic complex gel|

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US4808404A|1988-01-11|1989-02-28|A. H. Robins Company, Inc.|Live vaccine for coccidiosis utilizing coccidial sporozoites|

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US5178882A|1990-06-22|1993-01-12|The Regents Of The University Of California|Viral decoy vaccine|

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法律状态:

优先权:

申请号 | 申请日 | 专利标题

GB838326633A|GB8326633D0|1983-08-04|1983-08-04|Compositions|

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