Method of purifying human beta-interferon

专利摘要:
A method for the purification of human interferon by the successive treatments of a crude interferon solution with an immobilized carrier which is coupled with a blue dye and a chelating carrier which contains a chelating residue chelated with at least one metal ion selected from the group consisting of Co2-. Ni2- and Zn2-.
公开号:SU1523046A3
申请号:SU833593070
申请日:1983-05-16
公开日:1989-11-15
发明作者:Хосои Кацуо；Озава Хитоси
申请人:Торэй Индастриз, Инк (Фирма)；
IPC主号:

专利说明:

This invention relates to medicine, in particular to a method for purifying human interferon, especially human (5-interferon.
The purpose of the invention is to increase the productivity of the process.
The method is carried out as follows.
The crude interferon obtained as a result of the activity of human cells is treated with immobilized blue carrier, InterLeron, absorbed on the immobilized blue carrier, is eluted with eluent. The interferon solution to be taken is then treated with a metal chelate carrier with a chelating residue, including 5 m at least one metal ion selected
14 24 out of a group consisting of Co, Ni and Zn. The interferon absorbed on the chelate-metallic carrier is separated from the carrier to obtain an interferon preparation of high purity and high concentration.
The crude interferon solution is first brought into contact with the immobilized blue carrier,.
The blue dye can be immobilized on any of a variety of commonly used carriers capable of combining with the dye. Such carriers include (A) a dye compound through the amino group of the anthraquinone moiety with agarose activated with cyan bromide; (c) combining a dye with a cross-linked agarose gel by a triazine compound via an ester linkage; (c) a compound of blue dextran R (dextran combined with a blue dye, the company Farmesie) with agarose activated by cyanogen bromide by the method of a triazine compound; (e) a compound of blue dye associated with
sd to
with
about
four
ABOUT
 CM
315
side chain Affi-gel 10® by Bio-Rad Lab. hereinafter referred to as Vio-Rad) via a peptide bond to a polysaccharide carrier. Other carriers can also be used, for example, a cross-linked dextran gel, for example, Sephadex, (Pharmesie), and a hydroxyl vinyl polymer that can also be used as a carrier for chelate metal chromatography in the next stage; , 1st granulated polymer, for example, Toyoperl ,; (firm Toyo soda comp.).
It is preferable to use a blue agarose gel, appropriate. I; B), since it is very efficiently bound to interferon, does not cause separation of the dye from the carrier, since the compound of the dye with the carrier is very stable under pH conditions from 6 to 13, and it is readily available on the market. This blue agarose gel has the following structure and is marketed under the trade name Sepharose Cb-bv {Pharmesie firm) Matrex gel A® blue (Amicon corp.) And Affi-gel blu® (Vio-Rad)
About NH-i
SOaH.,.,
TSIH N. /
t NH4ON Cross svN. Zannaya agaro80zN oh
gel
Either the batch method or the column method can be used to contact the immobilized blue carrier with the solution of the crude interferon.
Example 1. The solution of crude interferon byp was obtained by treating human fibroblast cells in MEM Needle medium containing 0.4% methylcellulose with poly 1; poly C, followed by treatment with cycloheximide and actinipin D,
21 mg of protein and 100 μg of actinomycin D per 1 l of 30 ml of blue agarose gel (Affigel blu® by Vio-Rad) were added to a 30 liter solution of crude interferon with interferon activity 6.2 and 10 ipedo. After stirring for 40 hours and
holding the supernatant for 3 hours was removed, and the agarose gel was transferred to the column, washing with a physiological solution containing phosphate-acid buffer. The supernatant was recovered again. The column was washed twice and the following washes and eluent were used:
first wash solution (320 ml):
1.0 M sodium chloride solution containing 10 mM phosphorus. sodium sulfate fpH 7.2) second wash solution (280 ml):
25% ethylene glycol solution containing 10 mM sodium phosphate and 1.0 M sodium chloride (pH 7.2) eluent (400 ml):
55% ethylene glycol solution containing 10 mM sodium phosphate and 1.0 M sodium chloride (pH 7.2) o
The yield of partially purified interferon in the eluate (300 ml) of 81% of the original solution of interferon, purification of 33 times.
In the column with zinc chelate (10 mp), the eluate (285 ml) and gel columns of blue agarose were passed at a flow rate of 20 ml / g. After double washing the column was eluted. The following washing solutions and eluant were used:
first wash solution (300 ml):
distilled water and O, D M sodium phosphate (pH 6.7) were used at a flow rate of 30 ml / g for 1 h alternately
a second wash solution (50 ml) g of 20 mM sodium citrate solution (pH 5.0); eluent;
0.1 M buffer solution of acetic acid - sodium acetate (pH 4.5).
Code of chelatz chromatography is 87%, and the total yield is 70% “Cleaning reached ε 330 times.
The activity and yield of interferon, the amount of protein and the specific activity of each stage are shown in Table 1,
Interferon activity analysis by electrophoresis of polyacrylamide gel
Part of the final eluate was dialyzed in the presence of sodium dodecyl sulfate (VAT) and lyophilized. Field of lyophilized dialysate reduction 2-mercaptoethanol The recovered material was subjected to electrophoresis using a polyacrylamide gel in the presence of VAT according to the method of Lampi (Neucher, 227, 680-635 (1970) for analyzing the activity of interferon and dye with about. 00 and brilliant blue of R 200. As a result, the activity of interferon and the dark blue band were detected only in the position corresponding to the molecular weight about 23,000 o
Analysis of actinomycin D „
The analysis of actinomycin D was carried out by the method of biological samples.
A portion of the final eluate was desalted by gel chromatography using Sephadex G 25® after the addition of human serum albumin (1 mg / ml). Adding additionally a small amount of human serum albumin and lactose, the desalted zlyuat was filtered through a 2 μm filter and lyophilized. Actinomycin D was detected in an amount of not more than 0.0003 μg / 10 and the amount of interferon activity,
The eluate from the blue agarose gel column contained; 0.7 µg / m actinomycin T To the total amount of actinomycin D was 210 µg. In addition, the presence of actinomycin D in the eluate from the gel column of blue agarose was detected by absorption (430 nm) and by bioassay
A portion of the eluate from the blue agarose gel column was desalted by gel chromatography using Sephadex G-25® after the addition of human serum albumin. The ethylene glycol was removed, the desalted eluate was lyophilized. Nevertheless, about 0.7 μg / 10 imoedo of the interferon activity of actinomycin D. was found.
Pyrogenic test on rabbits
The lyophilized material obtained by the above method from the final eluate was injected intravenously to three rabbits (i.e./kg
the mass of the rabbitJ, the summation of pyrexia for all three rabbits was 0.6 C. Interferon, purified by the proposed method, turned out to be negative to the pyrogenic rabbit test
Lyophilized material obtained from a blue agarose gel eluate was injected intravenously.
three rabbits (u / kg mass

rabbit / about summation of pyrexia for
all three rabbits were 1.5 ° C; Interferon, purified according to the blue agarose chromatography method, was found to be vaguely negative to the pyrogenic test on rabbits
Example 2. In this experiment a solution of crude interferon was used, similar to that used in Example 1.
20 l of a solution of crude interferon was passed through a 20 ml column of blue agarose (Matrex gel A blue, manufactured by Amicon Corp.). The column was washed three times and eluted. The following washing solutions and eluent were used:
first wash solution (200 ml):
 1 M sodium chloride containing 10 mM sodium phosphate (pH 7.2). second wash solution (200 ml):
25% ethylene glycol solution containing 10 mM sodium phosphate and 1 M sodium chloride (pH 7.2). third wash solution (40 ml);
A 40% ethylene glycol solution containing 10 mM sodium phosphate and 1 M sodium chloride (pH 7.2). eluent (200 ml):
55% ethylene glycol solution containing 10 mM sodium phosphate and 1 M sodium chloride
The chelatzik column (5 ml) was connected to the exit of the blue agarose column after the start of the elution, and the eluate from the blue agarose column was directly passed through the chelatzinc column. Then the chelate column was washed twice and eluted. Wash the internal solutions and the eluent were as follows:
the first wash solution (200 ml);
distilled water and 0.1 M sodium phosphate (pH 6.7) alternately second wash solution (20 ml)
.20 mM sodium citrate (pH 5.0): eluent:
0.2 M Acetic Acid-Acetic Acid-on-Gry buffer containing 1 M sodium chloride.
Interferon activity, yield, amount of protein and specific activity of each stage are shown in the scoreboard2,
PRI and MER 3, 20 l of a solution of crude interferon with interferon activity of 15-10 them, edo and 60 mg / l of total protein were in contact with up to ml of syneagarose carrier (Matrex gel Blue A® from Amikon CorPo). The carrier onto which interferon was adsorbed was loaded onto the column. Then the column was washed twice and eluted. The following washing solutions and eluent were used: the first washing solution (400 ml):
1M solution of sodium chloride containing 10 mM sodium phosphate (pH 7.2) and a second wash solution (400 ml):
25% ethylene glycol solution containing 10 mM sodium phosphate and 1 M sodium chloride (pH 7.2) and eluent; (400 ml).
A 100 mt solution of interferon taken from a column of blue agarose was passed through a 5 ml chelate column. The nickel chelate column used was prepared in the same manner as in Example 1, except that instead of a solution of zinc chloride, a solution of nickel chloride was used to flash the chelate column and elute. The following wash solution and eluent were used: wash solution
distilled water and 0.1 M sodium phosphate (pH 6.7, alternately used eluent:
0.2 M acetic acid-acetic acid buffer solution
0
five
0
five
0
five
0
five
0
five
sodium containing 1 M sodium chloride (pH 4.5). Interferon activity, yield, amount of protein and specific activity of each stage are shown in,
The final eluate contained a very small amount of pyrogenic substances and was negative relative to the test of Limulus. Actinomycin D was not detected in it.
Example 4 The procedure of Example 3 was repeated, except that a chelate cobalt column was used instead of a chelate column, and a chelate cobalt column was prepared as in Example 1, But a solution of cobalt chloride was used instead of zinc chloride,
The eluate (15 ml) contained 4.5–10 of them “interferon unit” yield 60%) and 0.25 mg of protein. The specific activity was 1.8–10 of them „unit / mg of protein. The final eluate was negative to the test. Limulus, Actinomycin D was not detected in it.
PR and Mer 5c cultivated. E. coli strain, in which the structural она interferon gene was integrated, and the cultured strain. Subject to the procedures for collecting bacteria, grinding bacteria, removing nucleic acid and precipitating ammonium sulfate. The protein fraction containing E-interferon obtained from the E. KOLI strain was dissolved in a 25% ethylene glycol solution containing 1 M sodium chloride and 10 mM sodium phosphate (pH 7.2) in order to obtain a solution of crude interferon. The solution of crude interferon had an interferon-like activity of 5 "10 them, unit. and 20 mg of protein per ml
40 ml of a crude interferon solution were passed through a column of 2 ml of blue agarose (Matrex gel Blu A of Amicon Corp.), equilibrated with sodium phosphate buffer solution containing 1 M sodium chloride O Column twice washed to remove approximately 95% of protein in the solution of crude interferon, and eluted with fractionation into each fraction of 2 ml. The following washing solutions and eluent were used:
first wash solution (10 mp): 25% ethylene glycol solution containing 1 M sodium chloride®
ri and 10 mM sodium phosphate
second wash solution (10 ml) 40% ethylene glycol solution containing 1 M sodium chloride and 10 mM sodium phosphate eluent:
60% ethylene glycol solution containing 1 M sodium chloride and 10 mM sodium phosphate
The eluate (12 ml) from the column of blue aga rose had an interferon activity of 1.6-10 units (80% yield) and 4.6 mg of bvcAo. The average specific activity in each fraction was 2.5-10 iMoed / mg protein ( maxo; 510 im.edo / mg protein).
The eluate was analyzed as in Example 1. As a result, the purity of the interferon activity with a molecular weight of about 19,000 was 10-50% (average 25%).
The 6 MP eluate from the blue agarose column was passed through a 1 mp chelate column equilibrated with a 60% ethylene glycol solution containing 1 M sodium chloride and 1 mM sodium phosphate. The column was washed three times and eluted. The following wash solutions and eluent were used: the first wash solution (6 ml): 60% ethylene glycol solution containing 1 M sodium chloride and 10 mM sodium phosphate. second wash solution (6 ml):
third water wash solution (6 ml) distilled water: 220 mM sodium phosphate buffer solution containing .2 M sodium chloride (pH 6.0), eluent:
0.1 M sodium acetate buffer solution containing 1 M sodium chloride (pH 4.0). The final eluate (4 mp) had an interferon activity of 4.0–10 imoeed, (yield 50%) and 0.4 mg of protein. The specific activity was 1-10 imo udo / mg protein.
The final eluate was analyzed as in Example 1 and a single band was found at a molecular weight position of about 19,000. The purity was greater than 97%.
Example 6. Was repeated pro- procedure of example 3, except

ten
five
0
five
0
five
0
five
0
five
that instead of a chelating column, a chelate zinc column was used and a 0.2 M solution of Ij-histidine was used as an eluent, a balanced sodium chloride (pH 7.0).
The eluate (20 mp). 50-10 and interferon (yield 67%) and 50 g of total protein. The specific activity was 1.0-10 units / mg protein „
Pr and M. 7. The crude interferon obtained from human fibroblast cells was used, similar to the interferon used in Example 1.
To 20 l of a solution of crude interferon with the activity of interferon 4210 iMoed. CMoprex Gel Blue A® Amicon corp 30 MP gel of blue agarose was added to a 70 mg / l beel concentration. After stirring the mixture for 3 days and aging for h, the supernatant was removed and the blue agarose gel was passed through a column of 200 mp. 1.0 M sodium chloride solution containing 10 mM sodium phosphate with pH 7.2 (buffer A).
The column was then washed twice. Before elution of interferon to improve purification, a column was connected to the output of the above column. with 15 ml of fresh agarose gel, equilibrated with buffer A, containing 25% ethylene glycol O solution. After the third wash, the interferon was eluted. The following washing solutions and eluent were used:
first wash solution (200 mp) buffer A
the second wash solution (ZOO nl); buffer A, containing 25% ethylene glycol solution,
the third wash solution (120 ml), 3 M sodium chloride solution containing 30 mM phosphoric acid sodium (pH 7.2) and 30% -11NY ethylene glycol solution
eluent (600 mp):
Buffer A containing 55% ethylene glycol solution
The eluate fractionation solution Results are presented in tabLo4o
The second elution fraction was further purified by chelatz chromatography.
Buffer A containing 50% was added to a column with zinc chelate (15 ml).
ch152304
ethylene glycol solution. A portion (270 ml) of the second elution fraction from the above blue agarose column was diluted with 27 ml of buffer A to obtain a 50% ethylene glycol solution and passed through a zinc chelate column at a flow rate of 30 ml / h.
All operations were performed with
The temperature is 2-8 ° C. After double washing
ten
ten
the column was eluted. The following washing solutions and eluent were used:
the first wash solution (200 ml), distilled water and a 0.1 M phosphoric acid solution (pH 6.7) were used at a flow rate of 30 ml / h for 1 h alternately
Interferon from the eluate containing 40% ethylene glycol solution, from which column the blue agarose gel was subjected to the same analysis as in example 7 to determine its purity and to carry out a pyrogenic test.
Its purity was 60% and the result of testing Lumulus is positive.
Comparative example 2. Column
second wash solution (30 ml): 2o of zinc chelate (50 ml) equilibrated with 2 M sodium chloride solution containing 20 mM sodium phosphate (pH)
eluent
0.1 M acetic acid - sodium acetate with a buffer solution containing 0.1 M sodium chloride (pH 7.4). The activity, the yield of interferon, the amount of protein / protein and the rate of this cleaning procedure are presented in Table 5.
Interferon from a zinc chelate column was analyzed by electrophoresis of a polyacrylate amide gel ... in the presence of sodium dodecyl sulfate (SAT). Purity determination eluate was subjected to a pyrogenic test using Limulus test using endotoxin-detecting reagent. Interferon purity 90%, the eluate is negative to the test., Limulus when the content of interferon is 1.6-10 units / MPO
Comparative example .1. A second M agarose gel column (Matrex Gel Blue А4) was loaded with 2 M sodium chloride solution containing 20 mM sodium phosphate, pH 7.2 (buffer c).
Part ;; p ... cd (45 mp) of the second elution of ich Kb. -oH; ibi blue agarose gel, .uy description in example /, diluted
25
thirty
35
Wali using PBS. Untreated human fibroblast interferon, which had an interferon activity of 30 "10 IU / ml and a protein concentration of 70 mg / l, was passed through a zinc chelate column with a bulk velocity of 100 MP / h. After washing, the column was eluted. The following wash solution and eluent {
a washing solution (400 ml), distilled water and a 0.1 M sodium phosphate solution (pH 6.7) were used at a volumetric rate of 100 ml / h for 1 hour, alternating with eluent:
A 0.1 M buffer solution of acetic acid and sodium acetate containing 1.0 M sodium chloride (pH 4.7). The elution fraction on a zinc chelate column was used for further purification using chromatography on blue agar.
The blue agar column (5 ml) was equilibrated with 0.1 M sodium acetate buffer, containing 50 M sodium chloride. The elution fraction from the above zinc chelate column was passed through the column, with a blue a: gar, with a bulk velocity of 10 ml / h. After two times 40
5 ml of Buffer A and 75 ml of Buffer B and the washout were eluted. The following washing solution and eluant were used: the first wash solution (50 ml) of a 1.0 M solution of sodium chloride.
they let blue agarose gel through the second column at a flow rate of 20 MP / h. All operations were performed at 15-25 C.
12

A buffer containing 30, DO and 50% ethylene glycol solution, respectively, was passed through the column at a flow rate of 20 MP / Cho
The results of the second gel chromatography of blue agarose are presented in table 6.
Interferon from the eluate containing 40% ethylene glycol solution, from which column the blue agarose gel was subjected to the same analysis as in example 7 to determine its purity and to carry out a pyrogenic test.
Its purity was 60% and the result of testing Lumulus is positive.
Comparative example 2. Column
zinc chelate (50 ml) equilibrated5
0
five
Wali using PBS. Untreated human fibroblast interferon, which had an interferon activity of 30 "10 IU / ml and a protein concentration of 70 mg / l, was passed through a zinc chelate column with a bulk velocity of 100 MP / h. After washing, the column was eluted. The following wash solution and eluent {
a washing solution (400 ml), distilled water and a 0.1 M sodium phosphate solution (pH 6.7) were used at a volumetric rate of 100 ml / h for 1 hour, alternating with eluent:
A 0.1 M buffer solution of acetic acid and sodium acetate containing 1.0 M sodium chloride (pH 4.7). The elution fraction on a zinc chelate column was used for subsequent purification using chromatography on blue agar.
A column of blue agar (5 ml) was equilibrated with 0.1 M sodium acetate buffer, containing QM 1.0 M sodium chloride. The elution fraction from the above zinc chelate column was passed through the column, with a blue a: gar, with a bulk velocity of 10 ml / h. After two times
containing 10 mM sodium phosphate, pH 7.2 (buffer A), second washing solution of GSO ml buffer A, containing 25% ethylene glycol: eluant:
buffer A, containing 55% glycol,
The eluate solution was fractionated. The results are shown in table 7,
Comparative Example 3 “Crude fibroblast interferon, which had an interferon activity of 7.7-10 IU / ml and a protein concentration of 35 mg / l, was used as the starting material. This stock solution (2.0 L) was mixed with 5 g (17 ml) of CRG oshriks (porous glass beads with a pore size of 350 A) and the mixture was vigorously stirred for 13 hours. Then the mixture was passed through a glass filter and CRG beads. Packed in a column (diameter 0.9 cm) Next, the column was washed with 00 ml of PBS and then with a 0.01 M solution of glycine-HC1 at pH 3.5, and eluted with 0.3 M buffer of glycine-HC at pH 2.0, containing 0.1 mg / ml human serum albumin.
The eluates were dialyzed using 41 L of a 1 M NaC1 solution buffered with a 0.02 M phosphate solution (pH 7.4).
Dialyzed solution: passed through a zinc chelate column with dimensions of 0.6.7 cm, then the column was washed with 10 ml of a 1 M solution of sodium chloride, buffered 0.02 M phosphate solution (pY 7.4) and 10 ml of 0, 1 M sodium acetate buffer (pH 5.9). Then the column was eluted with 0.1 M sodium acetate buffer (pH 4).
The results are shown in table 8.
Srat.1nte. ipprrr. 4 Ispolzusyrpn cs
Orfeg: N, anplo pchmGg ispolzimok; g neither: - {in the example
PiirTBop crude whit 7rfgfon, which has an itgurferon activity of 38-10 units / l 5 protein concentration 63 mg / l and specific activity 10) / mg protein, adjusted to pH 2 by adding 6 n. NA and loaded into the column- SP-Sefadekg.a (20 ml, 2 cm "KB, 4 cm). After g. Loading 6 l solution0
five
0
five
0
five
0
five
0
five
The crude interferon with a flow rate of 40 MP / hr, the column was washed with 2PO ml of distilled water. The column is then eluted with 320 ml of a 0.1 M sodium phosphate buffer solution (pH 8.3). The eluate solution is fractionated. The results are shown in Table 9o. The second and third elution fractions are used for the subsequent purification method by chelatzinc chromatography.
A column with zinc chelate (3 ml, 1 cm 3.8 cm) is equilibrated with 0.1 M sodium phosphate buffer (pH 8.3). The above fractions (ZOO ml) from the 3P-Sephadex column are passed through a chelatzinc column at a flow rate of 6 ml / h. After washing, the column was eluted twice. The following wash solution is used: thief and eluent: with
first wash solution (40 ml): distilled water and M sodium phosphate solution (pH 6.7 | used alternately; at a flow rate of 6 ml / h for 1 h; second wash solution (b ml): 2 M sodium chloride containing 20 mM sodium phosphate (pH 7.2) eluent:
0.1 M buffer solution of acetic acid - sodium acetate. Containing 0.1 M sodium chloride (pH 4.7). The results of this purification method are shown in Table 10.
The crude interferon solution used as the starting material has a pH of 7.3 °. After settling for 10 days, its interferon activity is 3810 nU / l and no change in the activity of the inter-. farona not detected. Whereas after settling for 10 days a solution of crude interferon having a pH of 2, before loading it onto an SP-Sephadex column, the interferon activity decreases to 16 10 units / l. This means that the solution of the crude interferon is unstable at pH 2o
The invention has the following significant features and advantages compared with the known technical solution.
Cleaning method in accordance with the known technical solution including
reads SP-Sephadex column chromatography followed by chelate metal chromatography. Crude interferon, purified in accordance with a known method, has a low concentration, for example, crude interferon used in example 1 has an interferon activity of 3.2-10 em, / l and a protein concentration of 20 mg / l, and crude interferon used in example 2 has interferon activity 5-10 im./l / l and a protein concentration of 25 mg / l. A known technical solution refers to the purification of crude interferon with a relatively low concentration of about
The proposed purification method includes chromatography on an immobilized blue carrier followed by chelate metal chromatography. In accordance with this purification method Highly concentrated crude interferon (for example, crude interferon | used in example 7 has interferon activity 42 10 IMoDo / L and a protein concentration of 70 mg / l. Can be satisfactorily purified
In addition, as can be seen from Example 7, in which high concentration of crude interferon was used, product extraction by a known method was at most 47%. Thus, the known method does not allow the purification of high concentration of crude interferon. This becomes more apparent from a comparison of the results of the example 7 with the results of comparative example 4 (in both the samples, the dp material was a crude raw material of interferon with a high concentration,
Purifiers Stadium Purifiers Stadium
7 69
36 20
The application of the known method is limited to crude interferon with a relatively low concentration. The proposed method is applicable to crude interferon as a low concentration.
five
0
0
five
0
35
45
5U
55
radio transmitter, and with a high concentration of
The known method has the disadvantage that, before loading the SP-Sephadex column, the crude interferon is necessarily made strongly acidic since the interferon activity in this acid state is noticeably lost, as shown in comparative example 4
This disadvantage of the known method is not found in the invention since the chromatography on the immobilized blue carrier is carried out under conditions of almost neutral pH.
Example A Untreated interferon solution used is obtained by straining human fibroblast cells using a technique similar to Example 1,
To a solution of crude interferon, which has an interferon activity of 30,000 u / l, a protein concentration of 0.11 mg / mp and a pH of 7.2, was added 6 n HCl solution or I But NaOH solution in order to bring the pH to up to 2.3, 4,5,6,7,8,9 or 10.
The stability of each solution of the crude interferon is used by determining the titer remaining after settling each solution of the crude interferon at a temperature of 16 hours.
Below are the results of the test, expressed in percentage
pH SUSTAINABILITY%
283
369
480
590
697
7100
898
994
1080
Then 15 ml of each solution of the crude interferon is placed in a polypropylene centrifuge tube, in which 0.05 ml of blue dye as a carrier (Matrex Gel Blue / P) is also placed and the mixture is stirred for 16 hours. After removing the supernatant layer and washing twice 2 , 5 ml of 10 gM sodium phosphate solution - 1 But NaCl (pH 7.2) 2 ml
10 mM sodium phosphate - 1 N NaCl (pH 7.2) containing 55% ethylene. the glycol is added to the carrier. After centrifugation, the supernatant layer containing the partially purified interferon is removed.
The amounts of the lost interferon are then determined. The total content of interferon not absorbed onto the carrier and the interferon contained in the washing solution and the reduced interferon.
Below are the results expressed in percentages.
pH
2 3 4 5 6 7 8 9 10
0
five
0
five

权利要求:
Claims (1)
[1]
As can be seen from the above results, when the pH of the solution of unpurified interferon was increased to 5-9, the solution of unpurified interferon remained stable and recovery of partially purified interferon was high. Invention Formula
The method of purification of human α-interferon by contacting the crude interferon solution with a sorbent, processing the signal with an eluant with subsequent metal-chelate chromatography of the resulting eluate, in which the carrier contains a hepato-forming residue, and as an ion with Co or N1 or Zn and eluting the target product of histidine or acid buffer solution, characterized in that, in order to increase the productivity of the process, a blue agarose gel is used as a sorbent, and the contacting is carried out at pH 5-9 o
Table 1

st -

R

and that
30,000 186
32,000 320 280 300
285 285 300
50 10 20 30
3
0.2 1.4 150
143
five
0.6
10 60 64 124
81
32 34 10
630
570 20 15 15
14
7
1.8
four
1.0 0.16 1.2
3,010
1.0-10
2.5-1p 6-10 4-10 1.0-10
6
s -


20,000 300
400
100
20
260
65 0.7
55
Table 2
T a b l and c a 3
1200
2,
872701.0-10
740.55
The solution of crude interferon Missed through
300
A solution of crude interferon. Missed through
column
Elution
30% solution
ethylene glycol
40% solution
ethylene glycol
50% solution
chyle glycol
Table
Table3
390
15.4
2500
Table
2.5 1.6 1.7
2500 60
11 0.21 3300 42 0.15 17000 8 0.07 7100
Skipped over +.
1 elution fraction
2- elution fraction 1; s b l and c a 7
Tables
10.3-10
1,3-10
69

类似技术:

---

公开号 | 公开日 | 专利标题

---

SU1523046A3|1989-11-15|Method of purifying human beta-interferon

---

US4257938A|1981-03-24|Purification method of human fibroblast interferon

---

JP3768485B2|2006-04-19|Purification method of serum albumin

---

CA2050277A1|1990-10-15|Process for purifying a protein

---

Danishefsky et al.1976|Synthesis of heparin-sepharoses and their binding with thrombin and antithrombin-heparin cofactor

---

CA1101847A|1981-05-26|Process for the concentration of a placenta-specific glycoprotein

---

US5849874A|1998-12-15|Process for the purification of serum albumin

---

JP2573467B2|1997-01-22|Pharmaceutical compositions suitable for therapeutic use containing factor IX protein

---

US4065445A|1977-12-27|Pregnancy-specific β1 -glycoprotein and process for isolating it

---

EP2894159A1|2015-07-15|Mix-mode antibody affinity separation matrix and purification method using same, and target molecule

---

AU682560B2|1997-10-09|Purification of vitamin-K dependent proteins by membrane chromatography

---

US6555661B1|2003-04-29|Simple, environmentally benign, method for purifying protein A

---

EP0089218B1|1989-01-04|A process for purifying human chorionic gonadotropin

---

US5698104A|1997-12-16|Purification process for hirudin using affinity chromatography

---

Kawade et al.1981|[74] Purification of L cell interferon

---

SU551339A1|1977-03-25|The method of purification of enzyme preparations

---

US4723000A|1988-02-02|Human interferon gamma and interleukin-2

---

JP2661790B2|1997-10-08|Purification method of urinary trypsin inhibitor

---

JP2882828B2|1999-04-12|Method for purifying human urinary trypsin inhibitor

---

JP3508149B2|2004-03-22|Human serum albumin and method for producing the same

---

CA1065305A|1979-10-30|PROCESS FOR THE ENRICHMENT OF THE PREGNANCY-SPECIFIC .beta.1-GLYCOPROTEIN

---

Kessner et al.1979|Isolation of human urinary protease inhibitor by single-step affinity chromatography

---

JPH084503B2|1996-01-24|Method for recovering urokinase and urokinase-containing fraction obtained thereby

---

JPH0679172A|1994-03-22|Chromatographic agent and protein and method for use thereof for separating polypeptide or metal

---

JPH05339297A|1993-12-21|Separation of ovotransferrin

同族专利:

---

公开号 | 公开日

---

JPH0112760B2|1989-03-02|

---

JPS58201794A|1983-11-24|

---

UA6303A1|1994-12-29|

---

CA1213216A|1986-10-28|

---

EP0094672A1|1983-11-23|

---

US4541952A|1985-09-17|

---

DE3361838D1|1986-02-27|

---

EP0094672B1|1986-01-15|

引用文献:

---

公开号 | 申请日 | 公开日 | 申请人 | 专利标题

---

---

DE11435C|M. MERZBACH in Berlin C, Seydelstrafse 9|Locks for suitcases, chats, books, bag hangers and so on by means of a letter lock|

---

DE27262C|H. HEWITT in Birmingham|Steel spring with ball point|

---

JPS5728270B2|1978-11-07|1982-06-15|

---

FI77877C|1979-04-20|1989-05-10|Technobiotic Ltd|Process for the preparation and purification of human Le-shaped interferon protein.|

---

US4278661A|1979-10-12|1981-07-14|E. I. Du Pont De Nemours And Company|Purification of interferon|

---

NL7907791A|1979-10-23|1981-04-27|Stichting Rega V Z W|METHOD FOR PURIFYING INTERFERON.|

---

US4289689A|1980-03-14|1981-09-15|Hoffmann-La Roche Inc.|Preparation of homogeneous human fibroblast interferon|NO811118L|1980-04-03|1981-10-05|Biogen Nv|DNA SEQUENCES, RECOMBINANT DNA MOLECULES, AND PROCEDURE FOR PREPARING POLYPEPTIDES|

---

US4723000A|1983-07-05|1988-02-02|Biospectrum, Inc.|Human interferon gamma and interleukin-2|

---

US4530787B1|1984-03-28|1993-07-20|Cetus Corp|

---

JPS6169799A|1984-09-14|1986-04-10|Kyowa Hakko Kogyo Co Ltd|Purification of interferon|

---

AT52425T|1985-02-04|1990-05-15|Hoffmann La Roche|ADSORBENS FOR PROTEIN CLEANING.|

---

US4960702A|1985-09-06|1990-10-02|Codon|Methods for recovery of tissue plasminogen activator|

---

US4732683A|1986-12-02|1988-03-22|Biospectrum, Inc.|Purification method for alpha interferon|

---

US4961969A|1987-05-11|1990-10-09|Cetus Corporation|Process for recovering microbially produced interferon-β|

---

US4808314A|1987-09-18|1989-02-28|Scripps Clinic And Research Foundation|Method for reducing bacterial endotoxin contamination in solutions of macromolecules|

---

US5597485A|1988-05-13|1997-01-28|Vilmax S.A.|Process for separating proteins|

---

US5034133A|1990-07-26|1991-07-23|Schering-Corporation|Purification of human interleukin-4 from a CHO-cell line culture medium|

---

US6479300B1|1999-03-15|2002-11-12|Millipore Corporation|Metal loaded ligand bound membranes for metal ion affinity chromatography|

---

WO2002025288A2|2000-09-22|2002-03-28|Clontech Laboratories Inc.|Highly sensitive proteomic analysis methods and kits and systems for practicing the same|

---

WO2002074806A2|2001-02-27|2002-09-26|Maxygen Aps|New interferon beta-like molecules|

---

US7176278B2|2001-08-30|2007-02-13|Biorexis Technology, Inc.|Modified transferrin fusion proteins|

---

KR100524871B1|2003-12-04|2005-10-31|씨제이 주식회사|Processes for the purification of interferon beta|

---

KR100524872B1|2003-12-04|2005-11-01|씨제이 주식회사|Processes for the purification of interferon beta|

---

DE102009032179A1|2009-07-07|2011-01-13|Biogenerix Ag|Process for purifying interferon beta|

法律状态:

优先权:

---

申请号 | 申请日 | 专利标题

---

JP57081505A|JPH0112760B2|1982-05-17|1982-05-17|LV930959A| LV5408A3|1982-05-17|1993-06-30|Decrease in human beta-interferon depletion|

---

LTRP1024A| LT2589B|1982-05-17|1993-09-21|THE HUMAN ALPHA -INTERFERENCE CHOICE|

---

MD94-0031A| MD48C2|1982-05-17|1993-11-12|Method for human b-interferon purification|