• 专利附录
  • 专利说明
  • 权利要求
  • 类似技术
  • 同族专利
  • 引用文献
  • 法律状态
  • 优先权
    • 专利摘要:
    Method and apparatus for analyzing whole blood samples which feature impregnating a gel medium used for the analysis of the whole blood sample with an inert substance. When the whole blood sample is placed on the gel medium, the inert substance diffuses from the gel as the plasma solutes of the blood diffuse into the gel. The amount of the inert substance diffusing from the gel medium is inversely proportional to the hematocrit of the blood sample. In one embodiment, plasma solutes are caused to react with one or more reagents in the gel medium to assay various specific analytes. Also, the hematocrit, indicated by the diffusion rate of the inert substance from the gel, is determined and used to correct such assay.
    公开号:SU1501929A3
    申请号:SU792789701
    申请日:1979-07-10
    公开日:1989-08-15
    发明作者:Дж.Ли Мартин；Дж.Малин Микаэль
    申请人:Текникон Инструментс Корпорейшн (Фирма)；
    IPC主号:
    专利说明:

    This invention relates to the field of medicine and can be used for component analysis of whole blood.
    The purpose of the invention is to improve the accuracy of the method.
    FIG. 1 is a graph showing the diffusion of vitamin B from agarose into whole blood as a function of hematocrit; in fig. 2 is a graph showing the diffusion of vitamin B from agarose as the square root of the time function according to the diffusion laws J in FIG. 3 is a graph showing the correlation between hematocritis
    (HTT), determined by diffusion and standard technical centrifuging techniques in FIG. 4 is a graph showing the effect of hematocrit on albumin analysis.
    The method is carried out in a skinny manner.
    Vitamin B preparation, in an agarose gel.
    A 25 ml Erlenmeyer flask is added. 0.1 g of agar and 9.0 ml of PBS (phosha-te beffered salim) containing 80 ml 0.1 M 20 ml 0.1 M:
    8.5 g of NaCl and 0.2 g of NaN ,, pH 7.3. The flask is placed in a boiling bath.
    WITH
    c
    CD
     CM
    on
    5 minutes to dissolve the aga. Cool the mixture to 50 ° C, add rose water.
    1.0 ml of vitamin 1.0% (in PBS) is poured thereto. Then gel sticks are made by injecting warm mixture of wells into the wells and block them with a plastic coating. The sticks can be stored at room temperature in a humidified container for several months. Red; color is stable during this period. ; Determination of blood hematocrit by diffusion of vitamin B from the gel to the outside.
    For each rod, the initial absorbance at 540 nm was determined with a clean (no additive) agarose gel as a reference. The sticks are then immersed for 10 minutes in 1.5 ml of whole blood of a known hematocrit (HST) determined by centrifugation. The rods are quickly rinsed in two beakers with pure PBS and the absorbance at 540 nm is again determined. The change in absorbance at 540 nm is then determined.
    1501929
    NaOH pellets), then Brij 5.0 ml - 35, 30% surfactant solution and 5.36 g of NaCl are added. The volume e of the mixture is adjusted to 1 l.
    Gel analysis for albumin.
    The initial absorbance of each stick at 630 nm is determined. 10 Place blood drops on the surface.
    15
    20
    25
    gel for 30 seconds, quickly wash the sample in two washes with clean succinate buffer. After 20 minutes, the absorbance of the stick was measured again at 630 nm.
    权利要求:
    Claims (1)
    [1]
    Plot & AJJP is plotted as a function of hematocrit (HST) with a fixed, swarm of plasma albumin concentration, which illustrates the hematocrit effect (Fig. 4). Invention Formula
    A method for analyzing a sample of whole blood by placing it on the surface of a gel, separating the solid and liquid phases, followed by analyzing the analytes inside the liquid phase and inside the gel, in that, with: the aim of improving the accuracy of the analysis, an agarose gel containing the dye, vitamin B, and the gel containing the reagent for the analyte, the analysis is performed spectrophotometrically, the hematocrit number is additionally determined by the amount of vitamin B diffusing from the gel, the analyte concentration is determined by the cta its color reaction, the concentration of which is adjusted in accordance with the defined by the hematocrit.
    LA
    .
    The experimental data are plotted on the graph, where bA is the y-axis, Hct is the abscissa axis (see Fig. 1-3 for data from 49 samples).
    Preparation of gels for analysis on albumin.
    In a 25 ml Erlenmeyer flask, 150 mg of agarose, 7.0 ml of dissolving succinate buffer, are placed. 8.85 g of succinic acid (75 mm) in 800 ml of distilled water (pH adjusted to 4.4 by adding
    gel for 30 seconds, quickly wash the sample in two washes with clean succinate buffer. After 20 minutes, the absorbance of the stick was measured again at 630 nm.
    Plot & AJJP is plotted as a function of hematocrit (HST) with a fixed, swarm of plasma albumin concentration, which illustrates the hematocrit effect (Fig. 4). Invention Formula
    A method for analyzing a sample of whole blood by placing it on the surface of a gel, separating the solid and liquid phases, followed by analyzing the analytes inside the liquid phase and inside the gel, in that, with: the aim of improving the accuracy of the analysis, an agarose gel containing the dye, vitamin B, and the gel containing the reagent for the analyte, the analysis is performed spectrophotometrically, the hematocrit number is additionally determined by the amount of vitamin B diffusing from the gel, the analyte concentration is determined by the cta its color reaction, the concentration of which is adjusted in accordance with the defined by the hematocrit.
    050 015
    FIG. I
    NMERSiOff TlMf
    60
    so w
    X 20
    Yu O
    10 10 X but 50 60
    CENTRiFUSM No Fia.Z
    CORRBCTfJJ FOK НсТ
    Ntircoffiecrfs ROCK No
    05
    类似技术:
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    SU1501929A3|1989-08-15|Method of analysis of whole blood sample
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    同族专利:
    公开号 | 公开日
    BE878245A|1980-02-14|
    SE7902734L|1980-02-25|
    AU518642B2|1981-10-08|
    GB2029012A|1980-03-12|
    CA1118327A|1982-02-16|
    JPS5531989A|1980-03-06|
    AU4727279A|1980-02-28|
    FR2434395A1|1980-03-21|
    NL7903582A|1980-02-26|
    DE2934110C2|1988-03-03|
    GB2029012B|1982-11-03|
    FR2434395B1|1985-02-22|
    JPS633260B2|1988-01-22|
    SE445146B|1986-06-02|
    US4250257A|1981-02-10|
    IT7924560D0|1979-07-23|
    CH640946A5|1984-01-31|
    IT1122264B|1986-04-23|
    DE2934110A1|1980-03-06|
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    法律状态:
    优先权:
    申请号 | 申请日 | 专利标题
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