• 专利附录
  • 专利说明
  • 权利要求
  • 类似技术
  • 同族专利
  • 引用文献
  • 法律状态
  • 优先权
    • 专利摘要:
    A new compound, named Substance No. 51262, may be produced by cultivating a suitable microorganism of the genus Streptomyces, especially the new strain SANK 63584 (FERM BP-958). The compound has herbicidal and growth retardant properties.
    公开号:SU1442063A3
    申请号:SU864027022
    申请日:1986-02-04
    公开日:1988-11-30
    发明作者:Ханейси Тацуо；Накадзима Муцуо；Ториката Акио；Оказаки Такао；Тодзигамори Манабу；Кавакубо Кацухико
    申请人:Санкио Компани Лимитед (Фирма)；
    IPC主号:
    专利说明:

    (21) 4027022 / 28-13
    (22) 04.02.86
    (31) 2060J / 1985
    (32) 05.02.85
    (33) JP
    (46) 11/30/88. Vul. No. 44
    (71) Sankio Company Limited (JP)
    (72) Tatsuo Haneisi, Mutsuo Nakajima, Akio Torikata, Takao Okazaki, Manabu Tojigamori and Katsuhiko Kavakubo (JP) (53) 576.8 (088.8)
    (56) US Patent No. 4263036, cl. A 01 N 63/00, 1981.
    (54) METHOD OF OBTAINING AIRCRAFT No. 51262
    (57) The invention relates to the microbiological industry, in particular to a method for producing a new herbicide, referred to as substance 51262. The purpose of the invention is to obtain a new, more effective herbicide. The method consists in the cultivation of Streptomyces hygrosco picus PERM actinomycete on a liquid nutrient medium containing sources of carbon, nitrogen in mineral salts, with subsequent removal of biomass and isolation of the desired product from the culture fluid and its purification. The obtained herbicide is more effective than biolafos, tab. 4, 3 Il.
    WITH
    with
    1 | tc
    oh oh
    with
     sn
    11442063
    The invention relates to the microbiological industry, in particular, to a method for producing herbicides, namely a new compound, referred to as substance No. 51262, which exhibits herbicidal activity and the ability to inhibit plant growth.
    The purpose of the invention is to obtain HOBO-JQ elements. In the process of cultivating a more effective herbicide.
    The structure of substance No. 51262 has not been adequately understood. It has the following characteristics:
    Appearance: neutral, colorless 5 crystals.
    Specific rotation: al +28.8 (, 04, Н20, D - line sodium). C, 38.54; H 4.62;
    N
    Calculated, 12.84. C-, H oN20g
    20
    Antifoaming agents such as silicone, vegetable oil, or suitable surfactants can be used in the liquid medium.
    The cultivation is carried out at neutral pH and a temperature of 24-38 ° C, most preferably at a temperature of about 28 ° C.
    The production of substance No. 51262 during cultivation is controlled by evaluating the inhibitory activity of the culture fluid in relation to sensitive plant species, such as Brassica Cara (L) var. Komatsuna (Komatsuna).
    Found,%: C 38.55; H 4.53; N12.9
    Crystal melting point: i87-189 ° C.
    Molecular weight 218 m / e.
    UV spectrum (see Fig. 1): there are no absorption maxima above 220 nm in an aqueous solution.
    The IR spectrum for the KB tablet is shown in FIG. 2.,
    The NMR spectrum (cG, ppm) was measured at 400 MHz in heavy water using tetramethylsilane as an external reference (see figure 3).
    Solubility: soluble in water, methanol and ethanol, insoluble in ethyl acetate and acetone.
    Color reactions: positive for reactions with sulfuric acid, anisaldehyde (sulfuric acid and potassium permanganate).
    Thin layer chromatography: Rf value of 0.3 on a silica gel plate No. 5715 (Merck Co., 50: 10: 2 by volume of ethyl acetate isopropanol and water.
    The method of obtaining herbalism No. 5126 is as follows.
    The strain of actinomycete Streptomyces hydroscopicus (FERMBP-958) AnK 63584 is cultivated in a liquid nutrient medium during displacement and aeration. The nutrient medium contains sources of carbon, nitrogen, and mineral salts. As a carbon source, glucose, maltose, sucrose, mannitol, molasses, glycerin, dextrin, starch, soybean oil, and cottonseed oil can be used, from nitrogen sources
    soybean, peanut, cotton flour, fish flour, corn extract, peptone, meat extract, pressed yeast, yeast extract, sodium nitrate, ammonium or ammonium sulfate, from the salts sodium chloride, phosphates, calcium carbonate and, if necessary, metal salts in the form of micro5
    0
    five
    0
    five
    0
    five
    0
    five
    Antifoaming agents such as silicone, vegetable oil, or suitable surfactants can be used in the liquid medium.
    The cultivation is carried out at neutral pH and a temperature of 24-38 ° C, most preferably at a temperature of about 28 ° C.
    The production of substance No. 51262 during cultivation is controlled by evaluating the inhibitory activity of the culture fluid in relation to sensitive plant species, such as Brassica Cara (L) var. Komatsuna (Komatsuna).
    The highest amount of substance No. 51262 is observed after cultivation for 60-120 hours. However, this period may vary depending on the conditions and methods of cultivation; a shorter or longer period of each particular case may be acceptable by experimentation using appropriate control methods.
    Substance No. 51262, soluble in water, released mainly into the culture fluid, can be isolated by removing the dry matter, including the mycelium, for example by filtration, preferably using filter media such as diatomite, or by centrifugation. It can then be infused from the separated liquid part by traditional methods based on its specific physicochemical properties and subsequent purification if necessary.
    Substance No. 51262 is separated from other products using an adsorbent, either by adsorbing impurities, or by adsorbing a substance- No. 51262, or by adsorbing one or the other, separately or together with the subsequent elution of substance No. 51262. A wide range of adsorbents can be used, in particular activated carbon, resin-like adsorbents such as Amberlite (registered trademark) XAD-2, XAD-4 or XAD-7 (manufactured by Rohm and Haas), and Diaion (registered trademark) HP 10, HP20, CHP20F or HP50 (manufactured by Mitz kill chemical industries). Impurities present in the liquid part can be removed by passing a solution containing substance No. 51262 through a layer or column with one or more of the mentioned adsorbents or adsorbing substance No. 51262 on one or more adsorbents, followed by elution with a suitable eluent, acetone or butanol with water
    Substance No. 51262 may be further purified by various means, for example, distribution column chromatography using a cellulosic product such as Avitsel (a registered trademark of Asahi Chemical Industries) or Sefa-dex LH-20 (a registered trademark of Pharmaceutical Products), Impurities, powder, granules, microgranules.
    40
    qi
    Those in the liquid part containing substance No. 51262 can also be removed by adsorbing them on various cation exchange resins (strong or weak), such as Dowex 50U (registered trademark of Dow Chemical products) or Amberlite 1KS-50 (registered the trademark of Rohm & Haas products) or on an anion exchange resin, such as Dowex G or Diaion W A10.
    Further purification of substance No. 51262 can be carried out using chromatography using gel (if necessary),
    Substance No. 51262 exhibits germicidal activity and the ability to inhibit plant growth. By herbicidal activity, it is meant that the substance inhibits the growth of plants and may ultimately lead to their death. On the other hand, the ability to inhibit the growth of plants means that the substance inhibits or inhibits the growth of plants, without giving such signs of herbicidal activity that would manifest in necrosis or death of plants. The reaction of the plant to the substance
    (manifestation of a herbicidal effect or a growth inhibition effect) varies depending on the application or application method, the concentration of the substance No. 51262, and the sensitivity of the treated plant to the substance. The compound exhibits herbicidal activity against various
    10 weeds and applicable before and after germination. Herbicide can be applied on the foliage of plants. The substance can inhibit the growth of plants without causing their death. It is suitable for
    15 preventing lodging of rice plants due to the shortening of the straw of the rice plant, to reduce the duration or frequency of harvesting, to sow the seedlings by slowing down the growth of grass and garden plants, to ensure the dwarfism of flowering plants, etc,
    Substance No. 51262 may be used alone or in a mixture with a carrier and / or additives. The concentration can be presented in various forms, for example, it can be made up in the form of a dust, a wettable powder, a water-soluble powder or a liquid preparation. It is not necessary to use the completely clean form of substance No. 51262 so that cleaning can be stopped at any stage. and the resulting raw substance may be consumed as an active ingredient of the composition.
    The carriers used in such a composition can be synthetic or natural, organic or inorganic, and are mixed with the compound to facilitate storage, transportation and handling of the active ingredient 45 or, in order to promote the ingestion of the active ingredient into the plant, B clay, tal diatomite, kaolin, bentonite, calcium carbonate, gypsum, synthetic precipitated silica, attapulgite, zeolite or pumice stone, such as synthetic and natural resins, can be used as solid carriers; coumarone to see la, alkyd resins, polyvinyl chloride, ester gum or xanthan waxes such as carnauba wax and parafin-, and other organic mat
    50
    (manifestation of the herbicidal effect or growth inhibition effect) varies depending on the method of application or application, the concentration of the substance No. 51262 and the sensitivity of the treated plant to the substance. The compound exhibits herbicidal activity against various
    weeds and applicable before and after germination. Herbicide can be applied on the foliage of plants. The substance may inhibit the growth of plants without causing their death. It is suitable for
    preventing lodging of rice plants due to the shortening of straw of a rice plant, to reduce the duration or frequency of harvesting, to sow the seedlings due to inhibition of growth of grass and garden plants, to ensure the dwarfism of flowering plants, etc,
    Substance No. 51262 may be used alone or in a mixture with a carrier and / or additives. Contrast can be presented in various forms, for example, it can be made in the form of dust, grain.
    wettable powder, water soluble powder or liquid preparation. It is not necessary to use the completely pure form of substance No. 51262, therefore the purification can be suspended at any stage, and the resulting raw substance can be used as the active ingredient of the composition.
    The carriers used in such a composition can be synthetic or natural, organic or inorganic, and mixed with the compound to facilitate storage, transportation and handling of the active ingredient or, in order to promote the active ingredient to aggravate. , Clay, talc, diatomite, kaolin, bentonite, calcium carbonate, gypsum, synthetic precipitated silica, attapulgite, zeolite or pumice, such synthetic and natural resin can be used as solid carriers. s as coumarone resin, alkyd resin, polyvinyl chloride, ester gum or xanthan, waxes such as carnauba wax or parafin-, and other organic mate0
    rials such as nutshells (nagfimer, walnuts and other nuts) or soybean powder. Water and alcohols such as methanol, ethanol, isopropanol or ethylene glycol are used as liquid carriers.
    The composition may contain a surfactant that promotes dispersion, wetting or distribution of the composition. Anionic surfactants are introduced in the form of sodium and calcium salts of ligninsulfonic acid, sodium oleate, dodecylbenzene. The vegetative hyphae of the strain ZAZ 5358 grow well, with branching, on cres branching, dah ISP for 14 days
    sodium sulfonate, dialkylsulfosuccinate sodium zincate, sodium, ammonium and amine salts of polyoxyethylene alkyl-aryl ether sulfates or polyoxyethylene alkyl ether sulfates or free acids or salts of polyoxyethylene alkylarylsh1-2 for mainly closed helixes, which are used for mainly closed helix sulfate or polyoxyethylene alkylaryl salts or mainly polyoxyethylene alkyl sulfonate for sulfate sulfate, or polyoxyethylene alkyl ether sulfates, or polyoxyethylene alkyl aromatic sulfates;
    Microscopic studies of air hyphae show simple branches. Spore chains are represented by
    phosphates; Of cationic surfactants, higher aliphatic amines and ztilen oxide condensates are used with us such as quaternary ammonium chlorides, N-alkylamine acetates and N-alkyl aminoxide oxides. From amphoteric surfactants use betaines and surfactants of the amino acid type. From nonionic surfactants, glyceride and sucrose esters of fatty acids, condensates of ethylene oxide with higher aliphatic alcohols, condensates of ethylene oxide with alkylphenols or apkylnaphthols, amides or ethoxylated amides of higher fatty acids, and fatty acids, as well as the clocks, are used. sorbitol, higher fatty acid esters of glycerioborate or ethoxylated glycerolborate, and copolymers of ethylene oxide with propylene oxide.
    The compositions may contain protective colloidal substances such as gelatin, gum arabic, :: casein, polyvinyl alcohol or carboxymethylcellulose, or thickeners such as sodium polyphosphate or bentonite. To enhance applicability and save labor, the composition can be combined with fungicides, insecticides, other herbicides, plant growth regulators, or fertilizers.
    The actinomycete strain used was identified by the Brief Definition of Bacteria Vergy, Mi, Mir, 1980 t. 2, Actonomycetes strain was assigned No. 8APK 63384. It was deposited at the Institute of Fermentation Research of the Industrial Science Agency of the Ministry of International Trade and Industry, Japan, where it was assigned PERM P-8004, and re-deposited at the Institute of Fermentation Research with assignment of the number FERM BP-958.
    The strain has the following characteristics.
    Morphological and cultural characteristics.
    Vegetative hyphae of strain ZAZ 5358 grow well, with branching, on cres branching, dah ISP for 14 days
    the fight is mainly a closed spiral, the surface structure of the chain of spores
    the fight is mainly a closed spiral, the surface structure of the chain of spores
    A microscopic study of aerial hyphae shows simple branches. Spore chains are co5
    wart folded No special organs are observed, such as sclerotia, fragmented vegetative hyphae or sporangia.
    Aerial mycelium is moistened and blackened during cultivation. Soluble pigment does not produce. In tab. Figure 1 shows the characteristics of the growth and colonies of the strain obtained after cultivation for 14 hours at 28 ° C on various standard media.
    Physiological properties:
    Hydrolyzes starch. Thins gelatine. Restores nitrate. Milk does not coagulate. Peptonized milk. Grows at a temperature (Wednesday 1) 11-4b with. The optimal growth temperature (medium 1) is 24-38 ° C. Growing on medium 1, containing 7% NaCl, with a content of 10% - no growth. Decomposes casein and tyrosine. Does not decompose xanthine. Melanoid pigment does not form on media 1, 3, 4. There is no melanoid pigment (medium 3). There is no tube of melanoid pigment (medium 4).
    Media used:
    Wednesday 1: agar on yeast and malt extracts (ISP2),
    Medium 2: Tryptone-Yeast Extract Broth (ISP 1),
    Medium 3: peptone-yeast-extract iron agar (ISP 6),
    Wednesday 4: tyrosine agar (ISP 7),
    The use of carbon sources was studied using a non-carbon-free cjpegy Pridham-Gottlieb after incubus
    five
    0
    five
    strain 6358A for 14 days. Since the strain SANK 63584 can grow in the basal medium without introducing an additional carbon source, it is rather difficult to accurately describe its ability to use carbon sources. The degree of relative use of carbon sources is shown below. As a control, the basal medium was used, indicated by the sign C -).
    Relation to carbon sources:
    D-glucose + L-arabinose i D-xylose i
    inositol D-mannitol is used.
    D-fructose +
    L-rhamnose sucrose - raffinose - control - - poorly used, - - not used).
    Whole cell components.
    The cell walls of the strain Streptomyces hygroscopicus 8AJ 63584 contain b, b-diaminopimelitic acid and glycine.
    Example 1. Strepto-nycessp culture. The strain SANK 63584 is inoculated into four 500 ml Erlenmeyer flasks, each containing 80 ml of medium having the following composition, in wt%:
    Glucose3
    DRYAAJE1
    Soyeva flour 3
    Calcium carbonate 0.4
    MgS04-7H-iO 0.2
    Antifoam
    (Nissan Dismous
    CB-442 manufactured by Nissan
    Chemical Industries) 0.01
    Water to pH 7.2
    before sterilization 100
    Then the strain is cultivated on a rocking chair at 220 rpm for 72 hours at 28 ° C.
    2 ml of the obtained culture are inoculated in 130 Erlemeyer flasks of 500 ml each, each of which contains 80 ml of the described medium, and cultured on a rocking chair at 220 rpm for 96 hours at. The resulting culture broths and washes are mixed, in a volume of 11.4 liters, mixed and 1 kg Celite 545 is added (a trademark on the products of the company Jonze Manville Products - the mixture is filtered to obtain 1 1 l of filtrate, having a pH of 7.1. Then the filtrate is passed through
    442063 .8
    a matographic column filled with 3 kg of activated carbon (a product produced by Vacre Pure Chemicals G Industries) for adsorption of substances
     ,
    No. 51262,
    The column is washed with 4.5 L of deionized water, and then the target is eluted with 15 L of 10% (by volume) aqueous acetone. 16 l of the active eluate is condensed by evaporation under reduced pressure, and then lyophized: to obtain 61 g of dry powder containing target substance No. 51262. The resulting raw powder can be used as such for agrochemical use.,
    Then the raw powder is adsorbed
    20 on a chromatographic column containing 500 ml of Avicel, which is pre-equilibrated with acetonitrile. After that, the column is metered with 2 L of acetonitrile, 3 liters of 97% 25% (by volume) aqueous acetonitrile and 6 L of 85% (by volume) acetonitrile. The eluate is collected in fractions of 1 l each. It is established that the target substance No. 51262 is present in
    30 fractions from the 6th to the 9th. These fractions are combined, condensed by evaporation under reduced pressure, and then lyophilized to obtain 4.6 g of powder. The powder is dissolved in a small
    35 amount of 50% (by volume) aqueous methanol and adsorb on dex LH-20 in a column, which is pre-equilibrated with 50% (by volume) aqueous methanol. The column was eluted using 50% (by volume) aqueous methanol; the eluate was collected in 15 ml fractions. Fractions No. 36-50 contain the target substance No. 51262, they are collected, combined,
    45 is condensed by evaporation under reduced pressure and again passed through the same column with Sephadex LH-20. Eluted fractions were collected and freeze-dried to give 334 mg of sample No. 51262 of purity about 70%.
    300 ml of this sample is adsorbed on a silica gel column, which is pre-equilibrated with a 10: 1 mixture by volume of chloroform and methanol. The target substance is eluted with a 6: 1 mixture by volume of chloroform and meta. the eluate and the eluate are collected in 15 ml fractions. Frakischi number 22-60 collected.
    50
    55
    91
    combined, condensed by evaporation under reduced pressure, and then subjected to thin-layer chromatography on silica gel using a 50: 10: 2 mixture of ethyl acetate, isopropanol, and water as the solvent. The target substance is extracted with a 6: 1 mixture by volume of chloroform and methanol. The extract is condensed by evaporation under reduced pressure, and then lyophilized.
    25.5 mg of substance No. 51262 are obtained in the form of a colorless powder, having the following characteristics.
    Appearance: neutral, colorless powder.
    Specific rotation: ofJ + 24.4 ° (С 0.61,, D-line sodium),
    Calculated,%: C 35.59i H 5.08; N 11.86.

    Found,%: C 35.65; H 5.00; N 11.71, etc. all characteristics of substance No. 51262,
    Example 2. 260 mg of partially purified substance No. 51262 is prepared as in Example 1 (to the stage at which a raw sample is obtained with a purity of about 70%). This partially purified compound is dissolved in a small amount of water. The solution is adsorbed on a column containing 300 ml of Dione CHP-29P, and eluted with water. The eluate is collected in 5 ml fractions and the target substance No. 51262 is eluted in fractions No. 61-67, one spot on thin-layer chromatography. These fractions are combined, condensed by evaporation.




    under reduced pressure and lyophilized.
    Obtain 41 mg of target substance No. 51262 as a colorless powder. All specifications match.
    Example 3. Obtaining a crystalline substance.
    The procedure of Example 2 is repeated to obtain 130 mg of substance No. 51262 as a colorless powder. All of this powder is dissolved in hot acetone and cooled. The separated crystals are collected.
    37 mg of substance No. 51262 are obtained in the form of colorless needles. Characteristics match.
    Example 4. Obtaining substances in the form of granules.
    3
    ten
    After the cultivation of Example 1, the culture broth was adsorbed on a column of activated carbon. The active fraction eluted with 10% (v / v) acetone is evaporated to a residue under reduced pressure to give a crude powder. This / crude powder was dissolved in water to obtain a 50% (by weight) aqueous solution and was adsorbed onto granulated pumice stone (size 10-48 mesh according to Tyler (0.295-1.651 mm), to give granules.
    Example 5. Getting wettable powder.
    50 ma.ch. Raw powder, 3 ma.ch. sodium dodecylbenzene sulfonate, 2 wt.h.

    polyvinyl alcohol and 45 mah. clays are blended while being uniform and pulverized to obtain a wettable powder.
    Example 6. Obtaining substances in the form of a water-soluble powder.
    50 ma.ch. substances No. 51262, 2 May, h. substances No. 51262, 2 ma.ch. polyoxyethyl phenyl ether, 10 wt. synthetic silica and 38ma.ch. ammonium sulfate is uniformly mixed to form a water soluble powder.
    Example 7. Getting a liquid drug.
    For 10 hours, substances No. 51262 and 2 hours of sodium lauryl sulfate were uniformly dissolved in 88 parts of methanol to form a liquid preparation.
    Example 8, Obtaining a liquid preparation.
    10 parts of the substance No. 51262 and 2 parts of sodium dedecylbenzenesulfonate are dissolved in 88 parts of water to form a liquid preparation.
    Example 9. The effect of inhibition by komatsun
    Sterilized cotton wool is placed on the bottom of test tubes (10 X 100 mm) with a height of about 5 mm. In one tube, cotton wool is impregnated with 0.5 ml of diethized water, in others with an aqueous solution containing substance No. 51262 in various concentrations. Approximately 10 of them are placed in a test tube of matzun and incubated for 3 Å at 28 ° C to determine the inhibitory concentration of matter No. 51262, estimated by the presence or absence of germination of seeds n. The minimum inhibitory concentration of substance No. 51262 in relation to
    Komatsun Ow
    The research institute of seed germination with 3.13 µg / ml.
    Example 10. Test for emergence tillage.
    The plastic pot is filled with riding soil, providing a surface area of about 150 cm, then the seeds of subsequent annual weeds of Sefaria faberi Hernn sigitaria sanguinalis (L) Scorp, Echinochloa crus-galli (L) Beauv are sown on the soil. Setari viridis (L) Beauv, Ana ranthusretroflexus L, Brassica ajrven sis (L) Kuntrei Chenopodium album L, Ambrosia Artemsicfolia L, Abutilon theophrasti Medic, and Sida spinoaa are then covered with a layer of, chv.
    One day after seeding, an aqueous solution of substance No. 51262 is uniformly sprayed on the soil in an amount of 15 ml per pot. Then the pot is placed in the tesh for 3 weeks, after the expiration of which the roy is sleeping. The herbicidal effect of substance No. 51262 is assessed by comparing the growth of the treated plants with a control that was not treated.
    The results are shown in Table. 2
    Example 11. Treating leaves
    Weed seeds are sown in a pot and covered with a layer of soil and left in a greenhouse. 10 days after seeding, a water solution of the substance No. 51262 is mixed with 0.03 wt.% Grameen S distribution means (trademark of the Sankyo company). 5 ml of the solution obtained is sprayed into each pot. After 10 days after treatment, weed growth and the germicidal effect of substance No. 51262 are observed.
    The results of the experiment are presented in table. 3
    Example 12. Inhibition of plant growth.
    The plastic pot is filled with riding soil, providing a surface of the soil of 150 cm. You






    12 seeds of jellied rice, soy
    beans, corn and cotton. Tubers of perennial weeds, watered by umbilical (Cyperusrotondus), are planted in each pot and seeds and tubers are covered with the same soil. The pots are placed in the greenhouse for 10 days. Prepare aqueous solutions of substance P 51262 (according to example 11) to the required concentrations, and add 0.03% by volume of Grameen S distribution means, 5 mg of the resulting solution is sprayed onto the soil of each pot. Plant growth was compared after 2 weeks with a control that had not been subjected to such treatment. In plants treated with 125 ppm substance No. 51262, growth was inhibited in 50% of all plants, whereas in pots treated with 500 ppm. substance No. 51262, there was almost complete suppression of the growth of shoots and rhizomes nourishment.
    Example 13. Test substance No. 51262 for toxicity.
    A solution of substance No. 51262 was administered intravenously in the amount of 100 mg per 1 kg of body weight. The mice were observed for 14 days and no anomalies were detected.
    Comparative data on the effectiveness of herbicide No. 51262 and Bialaphos are presented in table. four.
    Tests were carried out according to Example 11 at a concentration of 250 ppm.
    权利要求:
    Claims (1)
    [1]
    Invention Formula
    The method of obtaining herbicide No. 51262 by cultivating the actinomycete streptomyces hygroscopicus ferm BP-958 in a liquid nutrient medium containing sources of carbon, nitrogen, mineral salts, with stirring and aeration, at a temperature of 24-38 ° C, followed by removal of the biomass and allocation of the target product from the culture - Tural fluid.
    13
    Saccharosonitrate Agar
    Saccharose-nitrate
    agar
    Glucono-aspartic agar
    Glycerin Asparagine Agar
    (ISP 5)
    Starch agar with inorganic salts (ISP 4)
    Tyrosine Agar (1SP 7)
    Peptone-Yeast Extract in Iron Agar (ISP 6)
    1442063
    14 Table 1
    Less good, flat, pale yellow-orange (2-9-9)
    Underdeveloped, velvety, brownish-white (1-6-6)
    Pale brown (2-8-9)
    Missing .
    Nice, flat, pale yellowish-orange (2-9-9) Slightly formed, velvety, white
    Pale yellowish orange (2-9-9) None
    Abundant, flat, pale yellowish-orange (2-9-9)
    Less good, velvety, pale yellowish-orange (2-9-9) absent
    Abundant, flat, pale yellowish brown (6-7-9)
    Abundantly formed, velvety, brownish-gray (1-5-6)
    Tan (6-6-8) None
    Abundant, flat, pale yellowish brown (4-8-9)
    Less good, velvety, white Tawny (6-7-8) None
    Abundant, flat, pale yellowish brown (4-8-9)
    ,
    Underdeveloped, velvety, white
    Water agar
    Agar on potato and carrot extracts
    Note.
    P - growth; OS - the reverse side, VM - aerial mycelium; RP - soluble pigments.
     The names of the colors and numbers are stamped according to the Guide to Color standard, published. Nippon shikisai kenkuse, Tokyo, Japan.
     -f
    brownish white (1-6-6) Yellowish brown (6-8-8) None
    Bad, flat, pale yellowish orange (2-9-9)
    Less good, velvety, light-measles-bad belmy (2-8-7)
    Light brownish gray (2-8-7) None
    Bad, flat, pale yellowish orange (2-9-9)
    Less horopsy, velvety, brownish gray (1-5-6)
    Light brownish white (1-7-6) None
    1714A206318
    table 2
    Note. Inhibition of growth of 0-5% - the efficiency of O;
    6-30% - i; 31-50% - 2; 51-70% - 3; 71-95% - 4; 96-100% - 5.
    T a b l and c a 3
    Broad-leaved weed Amaranthus retroflexus L.
    Note, a-m tly of one-petnya (roa annua) j b - chicken millet
    (echinochloa crus-galli); с - barren fire (bromufi sterillis); d - ergot (blackgrass) (alopecrus myoeurol- das); e - giant foxtail (setaria faberi); f - copro Alelpskoe (eorghuraHalepease); g-OBcnr (avena atue); h-lodmarnänk (galium arat1pe) {i - skolka (stellaria iKdia), j ark of 8canthium pensylvanicusn) - Field puney (viola ar)) Datyra ordinary (datyra stranoalum) jm - maria bel chenopodium album); n - bindweed purple (ipomoea purpu ea); o - Prickly eidb (side epinosa)} p - ambrosia polynolifolnna (ambrosia astemisii folia) i g canuka theofrast (abutilon theophrastis); r - white mustard (brassicaarvensis).
    Continuation of table 3
    Table 4
    g7% / g / vr
    90
    W 250 300 350 JTO
    ftM
    FIG. one
    tIIII
    000 360ff 280O 20OO 1800 ISOO 12OO 1OOO HEI 650
    F1 / g.2
    cm
    - /
    II
     650
    cm
    - /
    eight
    0
    flfi / L
    Tszig.z
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    RU2545403C2|2015-03-27|Compound a-87774 or its salt, method of obtaining thereof and agrochemical, containing thereof as active ingredient
    US4273571A|1981-06-16|Organic chemical compound, microbiological process for its preparation, and its use as a herbicide
    EP0332216B1|1992-11-25|Novel substance for agricultural use and process for its production
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    JPH07304622A|1995-11-21|New insecticidally active substance sf 2,775 substance, its production and insecticide comprising the same as active ingredient
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    同族专利:
    公开号 | 公开日
    AR240943A2|1991-03-27|
    JPH0613542B2|1994-02-23|
    AU597243B2|1990-05-31|
    DK56586D0|1986-02-05|
    EP0232572A2|1987-08-19|
    DK172686B1|1999-05-31|
    US5064760A|1991-11-12|
    IL77796A|1990-03-19|
    AU5323486A|1986-08-14|
    PT81976A|1986-03-01|
    EP0232572A3|1988-08-17|
    JPS6212789A|1987-01-21|
    CA1253096A|1989-04-25|
    PH22475A|1988-09-12|
    ZA86827B|1986-10-29|
    ES551678A0|1987-07-01|
    FI81834B|1990-08-31|
    FI860525A|1986-08-06|
    FI860525A0|1986-02-05|
    ES8706816A1|1987-07-01|
    BR8600457A|1986-10-21|
    DE3683031D1|1992-01-30|
    US4952234A|1990-08-28|
    FI81834C|1990-12-10|
    EP0232572B1|1991-12-18|
    PT81976B|1988-07-01|
    DK56586A|1986-08-06|
    AT70562T|1992-01-15|
    AR240943A1|1991-03-27|
    引用文献:
    公开号 | 申请日 | 公开日 | 申请人 | 专利标题
    RU2545403C2|2009-08-18|2015-03-27|Мицуи Кемикалз Агро, Инк.|Compound a-87774 or its salt, method of obtaining thereof and agrochemical, containing thereof as active ingredient|ZA737247B|1972-09-29|1975-04-30|Ayerst Mckenna & Harrison|Rapamycin and process of preparation|
    US4181715A|1975-12-29|1980-01-01|Meiji Seika Kaisha Ltd.|Novel antibiotic substance SF-1540 and its derivative, and process for the production thereof|
    EP0002695B1|1977-12-24|1981-11-04|Bayer Ag|Organochemical compound, process for its preparation, its use, compositions containing it, and microorganisms|
    US4320135A|1980-05-19|1982-03-16|Sandoz, Inc.|Inhibiting growth hormone secretion with 5,5-substituted hydantoin derivatives|
    US4415669A|1981-12-07|1983-11-15|Merck & Co., Inc.|Substance and process for its production|
    EP0170006B1|1984-06-05|1992-07-08|American Cyanamid Company|Method and compositions for helmintic, arthropod ectoparasitic and acaridal infections with novel agents|US2580216A|1943-06-08|1951-12-25|Bata Narodni Podnik|Device for nailing heels from the interior of shoes|
    IT1246379B|1990-04-12|1994-11-18|Ministero Del Uni E Della Rice|STREPTOMYCES NCIMB 40277 ACTIVE IN THE BIOSTIMULATION OF AGRICULTURAL PRODUCTION|
    US5354868A|1993-10-21|1994-10-11|American Cyanamid Company|Process for the preparation of -hydantocidin and analogs thereof|
    US5714599A|1994-06-24|1998-02-03|Novartis Corporation|Process for the preparation of ste specific 1'-spiro-nucleosides|
    法律状态:
    2007-09-20| REG| Reference to a code of a succession state|Ref country code: RU Ref legal event code: MM4A Effective date: 20040205 |
    优先权:
    申请号 | 申请日 | 专利标题
    JP2060385|1985-02-05|LV931278A| LV5696A3|1985-02-05|1993-11-26|Method of obtaining Herbicida No.51262|
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