Process for preparing the extract of green tea containing a large amount of polyphenol

专利摘要:
PURPOSE: A process for preparing cost effectively the extract of green tea containing a large amount of polyphenol without loosing activity of polyphenol is provided. CONSTITUTION: Polyphenol is a main component in green tea and has the following properties: antioxidation, anticholesterol, lowering blood sugar level, anticancer, and inhibiting agglomeration of blood platelets. The process for preparing the extract of green tea comprises the steps of: adding 60% to 80% of ethanol to leaves of green tea; first extracting at 85°C for 3 to 4 hours, followed by cooling at room temperature, and filtering; repeating the first extracting procedure; concentrating the extracts under vacuum condition; and reconcentrating the extracts to 1/6 to 1/15 volume under vacuum condition.
公开号:KR20000046079A
申请号:KR1019980062755
申请日:1998-12-31
公开日:2000-07-25
发明作者:김완기；이병곤；정진오
申请人:서경배；주식회사 태평양；
IPC主号:

专利说明:

Method for preparing green tea extract with high polyphenol content
The present invention relates to a method for producing green tea extract having a high polyphenol content.
Since ancient times, green tea has been widely consumed as a health beverage in the private sector, and recently, as the pharmacological mechanism of the various ingredients contained in green tea is gradually revealed, its value is re-recognized. Polyphenols, caffeine, amino acids, It contains a large amount of useful ingredients such as vitamin C. It has been found to be excellent for hangover and nicotine detoxification, fatigue recovery, cardiovascular action, and skin beauty. Cholesterol action, hypoglycemic action, anti-tumor action, platelet aggregation inhibitory effect and the like has been attracting much attention.
Accordingly, various production techniques for efficiently extracting polyphenols from green tea and purifying them with high purity are also proposed.
As a manufacturing method of the green tea extract known so far, for example, Japanese Patent Application Laid-Open No. 59-219284 (name of the invention: a method of purifying tea extract using a hydrophilic solvent), Japanese Patent Laid-Open No. 2-6499 (name of the invention: Molecular weight fractionation of tea extract with ultrafiltration membrane, Japanese Patent Application Laid-Open No. 2-311474 (name of the invention: a method of purifying tea extract with a column), Japanese Patent Application Laid-open No. 4-182479 (name of the invention: Method of purification using column and organic solvent), Korean Patent Publication No. 91-4203 (name of the invention; method of preparing high purity green tea extract and its use; 10 times by weight of water is added to green tea to 70 ~ 80 ℃ Heat extraction, cooling and filtration to obtain an extract, which is characterized by evaporating the organic solvent layer under reduced pressure by adding and fractionating ethyl acetate, 1-butanol, 2-butanol or 2-butanone of the same weight); Korean Patent Notification No. 93-5694 (Invention name: Manufacturing method of natural antioxidant: Extract the green tea leaves with water containing calcium carbonate, adsorb the extracted solution on the adsorption resin, elute with organic solvent, convert it to ethyl acetate, and concentrate it. To obtain a catechin powder).
However, after first extracting the green tea leaves by dipping and heating the mixed solution of hot water or alcohol, the extract is concentrated under reduced pressure, and the polyphenol is purified secondly using an organic solvent such as ethyl acetate and chloroform. The well-known method of extraction, since the polyphenol content of the primary extraction is as low as about 25 to 30%, there is a problem in that the cost and time of the subsequent purification process are considerably required and there is a limit in the overall manufacturing efficiency. In addition, since the method uses an organic solvent, there is a fear that the solvent may remain in the extract, and thus there is a problem that safety and working difficulties may arise. On the other hand, there is a problem in that a method of extracting an organic solvent by ethyl acetate or the like without first concentrating the extracted extract has a large amount of organic solvent. In addition, in the known method, polyphenol should be extracted at a high temperature of 80 ° C. or higher in order to obtain a high yield. In this case, since chlorophyll is contained a lot, a process of extracting and removing chlorophyll using chloroform is necessary. In this process, however, chloroform promotes oxidation of flavonoids, which is an active ingredient, and thus the active ingredient is lost, and there is a problem in that the waste of chlorophyll removed must be disposed of.
Accordingly, the present inventors have repeatedly studied to solve the above problems, when the green tea is extracted using 60 to 80% of ethanol to obtain a green tea extract containing a large amount of polyphenol component without a decrease in the activity of the polyphenol component In addition, the present invention has been found to provide a safe extract because it does not use an organic solvent.
Accordingly, it is an object of the present invention to provide a method for producing a green tea extract containing a large amount of polyphenols without degrading the activity of the polyphenol component.
In order to achieve the above object, in the method of the present invention, 60 to 80% ethanol is added to the green tea leaves, followed by primary extraction at about 85 ° C. for 3 to 4 hours, followed by cooling to room temperature and filtration to obtain a primary extract. step;
60-80% ethanol was added to the first extracted green tea leaves, followed by secondary extraction at about 85 ° C. for 3 to 4 hours, followed by cooling to room temperature and filtration to obtain a secondary extract;
Combining the primary extract with the secondary extract, and concentrating in vacuo to obtain a concentrated extract; And
Concentrating the extract again in vacuo again until it is 1/6 to 1/15 of the volume of the extract:
Characterized by including.
Hereinafter, the present invention will be described in more detail.
Since the method of the present invention uses ethanol which is an edible alcohol without using an organic solvent, it can provide a safe green tea extract, and can also provide a green tea extract containing a large amount of polyphenol without degrading the activity of the polyphenol.
The method of the present invention can also reduce the step of extracting at a high temperature of more than 80 ℃ but using chloroform for the removal of chlorophyll can prevent the loss of the active ingredient of the extract, it can provide the extract at a low cost.
Hereinafter, the present invention will be described in more detail with reference to Examples, but the present invention is not limited only to these Examples.
(Example 1)
1,800 g (8 times weight) of 60% ethanol was added to 100 g of green tea leaves, extracted at 85 ° C. for 3 hours, cooled to room temperature, and filtered to obtain a primary extract. Second extraction was carried out again in the same manner to the first extracted raw material, cooled to room temperature and filtered to obtain a secondary extract. The primary and secondary extracts obtained above were combined and concentrated by vacuum concentrator until the volume of the extract was 1/3. After cooling, the supernatant was collected and filtered, and then concentrated in a vacuum concentrator until the volume of the extract was 1/9 to obtain 30 g of the extract.
(Example 2)
1,800 g (8 times weight) of 70% ethanol was added to 100 g of green tea leaves, extracted at 85 ° C. for 3 hours, cooled to room temperature, and filtered to obtain a primary extract. Second extraction was carried out again in the same manner to the first extracted raw material, cooled to room temperature and filtered to obtain a secondary extract. The primary and secondary extracts obtained above were combined and concentrated by vacuum concentrator until the volume of the extract was 1/3. After cooling, the supernatant was collected and filtered, and then concentrated in a vacuum concentrator until the volume of the extract was 1/9 to obtain 28 g of the extract.
(Example 3)
1,800 g (8 times weight) of 80% ethanol was added to 100 g of green tea leaves, extracted at 85 ° C. for 3 hours, cooled to room temperature, and filtered to obtain a primary extract. Second extraction was carried out again in the same manner to the first extracted raw material, cooled to room temperature and filtered to obtain a secondary extract. The primary and secondary extracts obtained above were combined and concentrated by vacuum concentrator until the volume of the extract was 1/2. After cooling, the supernatant was collected and filtered, and then concentrated in a vacuum concentrator until the volume of the extract was 1/6 to obtain 28 g of the extract.
(Example 4)
1,800 g (8 times weight) of 80% ethanol was added to 100 g of green tea leaves, extracted at 85 ° C. for 3 hours, cooled to room temperature, and filtered to obtain a primary extract. Second extraction was carried out again in the same manner to the first extracted raw material, cooled to room temperature and filtered to obtain a secondary extract. The primary and secondary extracts obtained above were combined and concentrated by vacuum concentrator until the volume of the extract was 1/3. After cooling, the supernatant was collected and filtered, and then concentrated in a vacuum concentrator until the volume of the extract was 1/9 to obtain 26 g of the extract.
(Example 5)
1,800 g (8 times weight) of 80% ethanol was added to 100 g of green tea leaves, extracted at 85 ° C. for 3 hours, cooled to room temperature, and filtered to obtain a primary extract. Second extraction was carried out again in the same manner to the first extracted raw material, cooled to room temperature and filtered to obtain a secondary extract. The primary and secondary extracts obtained above were combined and concentrated by vacuum concentrator until the volume of the extract was 1/3. After cooling, the supernatant was collected and filtered, and then concentrated in a vacuum concentrator until the volume of the extract was 1/15 to obtain 23 g of the extract.
(Comparative Example 1)
1 kg of ethanol was added to 100 g of green tea, extracted at 70 ° C. for 2 hours, and then cooled and filtered. 760 g of the filtrate was evaporated under reduced pressure at 50 ° C. to obtain 26 g of concentrate. The concentrate was suspended in 300 g of water, fractionated with 300 g of chloroform to remove the chloroform layer, and the water layer was further fractionated with 300 g of ethyl acetate to obtain an ethyl acetate layer. This ethyl acetate etc. were evaporated under reduced pressure at 50 degreeC, and 5 g of extracts were obtained.
(Comparative Example 2)
1,800 g (8 times weight) of 50% ethanol was added to 100 g of green tea leaves, extracted at 85 ° C. for 3 hours, cooled to room temperature, and filtered to obtain a primary extract. Second extraction was carried out again in the same manner to the first extracted raw material, cooled to room temperature and filtered to obtain a secondary extract. The primary and secondary extracts obtained above were combined and concentrated by vacuum concentrator until the volume of the extract was 1/3. After cooling, the supernatant was collected and filtered, and then concentrated in a vacuum concentrator until the volume of the extract was 1/9 to obtain 32 g of the extract.
(Comparative Example 3)
1,800 g (8 times weight) of 80% ethanol was added to 100 g of green tea leaves, extracted at 85 ° C. for 3 hours, cooled to room temperature, and filtered to obtain a primary extract. Second extraction was carried out again in the same manner to the first extracted raw material, cooled to room temperature and filtered to obtain a secondary extract. The primary and secondary extracts obtained above were combined and concentrated by vacuum concentrator until the volume of the extract was 1/3. After cooling, the supernatant was collected and filtered, and then concentrated in a vacuum concentrator until the volume of the extract was 1/3. Thus, 34 g of the extract was obtained.
(Comparative Example 4)
1,800 g (8 times weight) of 80% ethanol was added to 100 g of green tea leaves, extracted at 85 ° C. for 3 hours, cooled to room temperature, and filtered to obtain a primary extract. Second extraction was carried out again in the same manner to the first extracted raw material, cooled to room temperature and filtered to obtain a secondary extract. The primary and secondary extracts obtained above were combined and concentrated by vacuum concentrator until the volume of the extract was 1/3. After cooling, the supernatant was collected and filtered, and then concentrated in a vacuum concentrator until the volume of the extract was 1/18 to obtain 20 g of the extract.
(Test Example 1)
The content of the polyphenols contained in the green tea extracts of Examples 1 to 5 and Comparative Examples 1 to 4 was measured, and the results are shown in Table 1.
The polyphenol content was measured by adding 20 mg of the green tea extracts of Examples 1 to 5 and Comparative Examples 1 to 4 into a 100 ml volumetric flask, followed by pouring 60 to 80 ml of water at 80 to 90 ° C., and then using 30 ° C. in a 80 ° C. water bath. Heat for minutes to dissolve the green tea extract. If the green tea extract does not melt well, dissolve it by sonication. It was then cooled in cold water, filtered to 100 ml solution. 1 ml of filtrate, 1 ml of iron stannate solution, and 3 ml of phosphate buffer solution were added to the test tube, followed by Box Rex mixing. Absorbance was measured at 540 nm, and the content of polyphenol was calculated according to the following equation. It was.
When the calibration curve is Y = AX + B (Y: ethyl gallate amount, X: absorbance value)
Polyphenol Content (%) = Y x 1.5 x 0.1 / Sample Weight (g)
Example 1Example 2Example 3Example 4Example 5Comparative Example 1Comparative Example 2Comparative Example 3Comparative Example 4 Polyphenol Content (%)28.9%29.3%31.4%32.8%35.5%60%28.2%27.3%34.5%
From Table 1, it can be seen that the method of the present invention can be effectively extracted polyphenols. On the other hand, the content of the polyphenol of Comparative Example 1 is higher than the method of the present invention, but there is a problem that the waste of chlorophyll removed because of the removal of chlorophyll using chloroform, Comparative Example 3 because of the high solid content There is a problem in that the flow is expensive because the extract is attached to the wall of the tank is not spray dried.
Test Example 2 Comparison of Activity for Removing Free Radicals from Cytoprotectants (using DPPH Assay)
1.0 ml of ethanol solution (200u) of green tea extract and 0.5 ml of methanol solution of free free radicals (DPPH sol., 1800u, 1,1-Diphenylpicrylhydrazyl) were mixed in a test tube, and after 20 minutes, the free free radical was removed at 517 nm. Was measured by absorbance, and the results are shown in Table 2. The lower the absorbance value, the better the activity of removing free radicals. The inhibition rate is a value obtained by measuring the absorbance at 517 nm after adding a sample to the DPPH free free radical solution and reacting to determine the amount of free free radicals remaining.
Sample nameusageAbsorbance (O, D)% InhibitionRemarks control Example 4 Example 5 Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 420u standard1.0360.1740.1650.1630.2540.2510.1678384848075.883.9
(Test Example 3)
After taking the green tea extract in a hard capsule was measured the change in the antioxidant capacity of the body. That is, two weeks after drinking wine, blood was collected before taking green tea extract capsules using sodium heparin tube and centrifuged for 5 minutes at 3000 rpm for 20 adults to separate plasma. It was. Then, after taking 500 mg of green tea extract capsules, blood was collected again using heparin sodium tube one hour later, and plasma was separated in the same manner as above. The separated plasma was immediately tested using Cobas Mira with a total antioxidant status kit (RANDOX Laboratories Ltd, UK), and the results are shown in Table 3.
mean total antioxidant capacity (mmol / L) Before taking green tea extract capsules1 hour after taking green tea extract capsule 1.36 ± 0.11.45 ± 0.1
As described above, according to the method of the present invention, it is possible to provide a green tea extract containing a large amount of polyphenols without any deterioration of activity, thereby utilizing not only the use as a natural antioxidant, but also the various physiological effects of the polyphenol component of green tea. Green tea extract of the present invention can be used in food, cosmetics and the like.

权利要求:
Claims (1)
[1" claim-type="Currently amended] 60 to 80% ethanol was added to the green tea leaves, followed by primary extraction at about 85 ° C. for 3 to 4 hours, followed by cooling to room temperature and filtration to obtain a primary extract;
60-80% ethanol was added to the first extracted green tea leaves, followed by secondary extraction at about 85 ° C. for 3 to 4 hours, followed by cooling to room temperature and filtration to obtain a secondary extract;
Combining the primary extract with the secondary extract, and concentrating in vacuo to obtain a concentrated extract; And
Vacuum concentrating the extract again until the extract volume is 1/6 to 1/15;
Method of producing a green tea extract, characterized in that it comprises a.

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同族专利:

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引用文献:

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公开号 | 申请日 | 公开日 | 申请人 | 专利标题

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法律状态:
1998-12-31|Application filed by 서경배, 주식회사 태평양

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1998-12-31|Priority to KR1019980062755A

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1998-12-31|Priority claimed from KR1019980062755A

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2000-07-25|Publication of KR20000046079A

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2002-09-18|Application granted

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2002-09-18|Publication of KR100344259B1

优先权:

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申请号 | 申请日 | 专利标题

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KR1019980062755A|KR100344259B1|1998-12-31|A method for preparing the extract of green tea containing high content of polyphenol|