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    • 专利摘要:
    The straw comprises a tube (11) having a swelling pad (15) provided with a fibrous carrier and a swelling agent associated with the support agent and configured to be at least in the vicinity of one second end (18) of the swelling pad, after a liquid-based substance has encountered its second end, in a swelling state thwarted by said tube such as if a first portion (26) of the swelling pad of length between 2 mm and 3 mm from its second end is exited from the tube by a second end (14) of the tube, while a second part of the swelling pad remains in the tube, the first part of the swelling pad is decompressed and blossoms with the second end of the swelling pad which takes a diameter (d) at least equal to one and a half times the inner diameter of the tube.
    公开号:FR3017793A1
    申请号:FR1451418
    申请日:2014-02-21
    公开日:2015-08-28
    发明作者:Eric Schmitt；Jean-Charles Gorges
    申请人:IMV Technologies SA；
    IPC主号:
    专利说明:

    [0001] The invention relates to straws for the preservation of a predetermined dose of liquid-based substance, in particular a biological substance, for example pure or diluted animal semen or a preservation medium containing embryos. It is known that such a straw is conventionally formed by a thin tube, having for example an internal diameter of 1.6 or 2.5 mm, and by a plug engaged in the thin tube. In the filled state, the plug is disposed near a first end of the tube and the dose of substance is disposed in the straw between the plug and the second end of the tube.
    [0002] To fill the straw, the first end of the tube, adjacent the plug, is placed in communication with a source of vacuum while the second end is placed in communication with a container containing the substance to be introduced into the straw. The air initially contained between the plug and the second end is sucked through the plug as the substance progresses in the tube until it meets the plug, which it can not cross because it becomes liquid tight. If necessary, after filling, the straw is welded in the vicinity of one or both ends and is stored cold. To empty the flake, if necessary after cutting the welded end portions and defrosting, is introduced into the tube through the end nearest the plug a rod that bears against the plug. With this rod, the plug is slid in the manner of a piston towards the end farthest from the plug, so that the dose of substance initially contained in the straw is expelled by this end.
    [0003] In general, the flake caps are of the tripartite type originally described in French Patent No. 995,878, corresponding to British Patent No. 669,265, that is to say formed by two pads of fibrous substance enclosing a powder which is converted on contact with a liquid in an impermeable paste or gel adheres to the wall of the tube so that the cap is liquid-tight. French Patent Application 2,651,676 proposes a stopper constituted by a first plurality of fibers and a second plurality of fibers associated with each other by braiding, the first plurality of fibers being constituted by polymerizable fibers under the action of the liquid and the second plurality of fibers being plurality of fibers being constituted by conductive fibers of the liquid by capillarity effect. The polymerizable fibers under the action of the liquid are made in the same material as the powder of conventional tripartite plugs. French Patent 2,753,367, which corresponds to US Pat. No. 5,868,178, proposes a tripartite plug whose length of the external buffer is at least two times greater than the length of the internal buffer. European patent application EP 0 873 726 proposes that the plug be made of a monoblock cylinder of hydrophobic microporous material. French patent applications 2,771,285 and 2,784,572, to which correspond the US patent application US 2001/0014376 and the US patent US 6,416,611, propose that the plug is constituted by a rigid insert pierced with a substantially coaxial orifice and a microporous and hydrophobic membrane associated with the insert to seal the orifice of the insert on the inner side. French patent application 2,781,662, to which corresponds US Pat. No. 6,203,489, proposes that the plug be constituted by a fibrous pad comprising a gelling material compound present in a dispersed gas-permeable form and comprising a support fiber compound, with the gelling material compound which is finely distributed throughout the carrier fiber compound, so as to allow swelling of the gelling material after contact with a water-containing substance, to form an integrated stopper with the support fiber compound . The gelling material is made of the same material as the powder of conventional tripartite stoppers. The gelling material swells by absorbing the water present in the liquid to completely fill the tube and then it reaches a gelling state while bonding with the support fiber compound to form an integrated plug. French patent applications 2,824,255 and 2,824,256, to which correspond the US patent applications US 2002/0183653 and US 2002/0188222, propose adding to the cap, in addition to the powder and fibers, non-polar elements. absorbents, in this case a thermoplastic core, coated with a sheath of braided yarns, and non-absorbent material in dispersed form, in the powder. The PCT application WO 2010/070533 proposes that the plug be made of a monoblock cylinder of self-sealing sintered microporous material as described, for example, in the PCT application WO 2010/070533, that is to say consisting of a microporous matrix and particles of substance with high water absorption capacity supported by the microporous matrix, which provides the block an intrinsic mechanical coherence (the particles do not detach) including in the moistened state. The invention aims to provide such a straw which is simple, convenient and economical to manufacture and which is efficient in use. The invention proposes for this purpose a straw for the preservation of a predetermined dose of liquid-containing substance containing water, in particular a biological substance, comprising a tube extending between a first end and a second end and comprising a a gas-permeable and liquid-tight swelling pad, which swelling pad is disposed in the tube adjacent its first end and extends between a first end facing the first end of the tube and a second end facing the second end of the tube which swelling buffer comprises a fibrous carrier and a swelling agent associated with the carrier, which swelling agent swells by water absorption in contact with the liquid-based substance, with said swelling pad and said tube which are configured so that after the liquid-based substance has come to meet the swelling pad by its second end The blowing buffer blocks the passage of the liquid-based substance and, by pushing on one end thereof slidable in the tube towards the second end of the tube; characterized in that said swelling pad is configured to be, at least near its second end, after the liquid-based substance has encountered its second end, in a state of swelling annoyed by said tube such as if a first part of the swelling pad of length between 2 mm and 3 mm from the second end of the swelling pad is output from the tube through the second end of the tube, while a second portion of the swelling pad remains in the tube, the first part of the pad The blister is decompressed and blooms with the second end of the swelling pad which has a diameter of at least one and a half times the inner diameter of the tube. The portion of the inflated buffer exiting the tube flourishes because the tube no longer resumes the tension born because the swelling has been thwarted and because in the absence of this voltage recovery part of the swelling buffer output tube is decompressed.
    [0004] The invention is based on the observation that certain substances with a high water absorption capacity, in particular superabsorbent polymers called SAP (SuperAbsorbent Polymers), although they are not wetting agents in the wetted state. and that, on the contrary, they tend to become quasi-liquid in view of the considerable quantity of water which they can absorb, may nonetheless serve as a swelling agent in a swelling pad to form a straw stopper, if their swelling is thwarted. adequately by the tube of the straw. It is in fact that by appropriately counteracting the swelling by the tube of the straw, the swelling agent can become so compact that it blocks the passage of the liquid and gives a mechanical strength to the swelling buffer, while the support agent, which is simply fibrous, and the swelling agent, which tends to become quasi-liquid, can not alone impart a mechanical strength to the swelling pad in the moistened state. It should be noted in this regard that in the above-mentioned part of the swelling buffer leaving the tube, which has decompacted, the swelling agent readily leaves the fibrous support agent, for example by simple wiping.
    [0005] It has been determined that the above-mentioned blooming characteristics are present when the swelling pad has in the vicinity of its second end a state of swelling which is adequately countered by the tube.
    [0006] The above-mentioned blooming features serve only to reflect the swelling state annoyed by the tube. It is understood that the straw according to the invention is used with the swelling pad which remains in the straw at its original location during the storage of the substance, and without leaving the tube at the end of the expulsion of the substance.
    [0007] Note that in the swelling buffer of the straw described by the French patent application 2,781,662, the maintenance of the consistency of the swelling buffer along the walls of the tube when the swelling buffer is slid into the tube is obtained because the swelling agent becomes in the moistened state a bonding agent, so that the swelling buffer becomes an integrated element.
    [0008] It will also be noted that in the plug made of a monoblock cylinder of self-sealing sintered microporous material described in PCT application WO 2010/070533 mentioned above, the maintenance of the coherence of the plug along the walls of the tube when the plug is slid into the tube is obtained because the microporous matrix retains the substance with high water absorption capacity. On the contrary, in the swelling buffer of the straw according to the invention, without the state of annoying swelling, the fibrous support agent and the swelling agent alone can not hold firmly to each other. when the swelling agent has absorbed liquid. As previously indicated, in the uncompressed state, the swelling agent readily leaves the fibrous support agent, for example by simple wiping. It is the tube-swollen swelling that causes the swelling agent to compact and remain associated with the fibrous support agent. It will be observed that the swelling buffer of the straw according to the invention is likely to have a particularly high swelling dynamic (speed of water absorption) and in this case, although it is surprising, the swelling agent blocks the passage of the liquid-base substance so rapidly that the amount of liquid-based substance consumed by the swelling buffer is very low, unlike the swelling buffer described by the French patent application 2,781,662, wherein the gelling material is made in the same material as the powder of conventional tripartite plugs, so that the performance in terms of amount of absorbed substance is similar to that of conventional tripartite plugs. It will be noted that the swelling buffer of the straw according to the invention is relatively simple, convenient and economical to manufacture, and in any case much more than the monoblock cylinder of sintered self-sealing microporous material 10 as described by PCT application WO 2010 / 070533. Finally, note that the upset swelling state of the swelling buffer provides a positional maintenance of the swelling buffer relative to the tube of the straw which is relatively high, and in any case much more than it is possible to obtain with the one-piece cylinder of sintered self-sealing microporous material described by PCT application WO 2010/070533. According to advantageous features of implementation of the straw according to the invention: - the swelling buffer is a braid formed by the combination of son; The swelling buffer comprises between eight and eleven threads per mm 2 of section of the tube of the straw; the swelling buffer has a weight of between 0.8 and 1.2 mg per mm of length; the swelling buffer comprises a proportion by weight of swelling agent of between 20% and 30%; the swelling agent is a superabsorbent polymer configured to absorb several hundred times its volume in water; the swelling agent is sodium polyacrylate; the swelling pad is formed by the combination of yarns each comprising support fibers and swelling fibers, said support agent being formed by the support fibers and said swelling agent being formed by the swelling fibers; the swelling buffer has a first predetermined color in the absence of prior contact with the liquid-based substance and a second predetermined color, having a hue different from the hue of the first color, when it has been in contact with said substance ; the swelling buffer comprises a non-fluorophoric salt in the dry state and fluorophore when it is dissolved in water; said salt is part of the group comprising a fluorescein salt, a Rhodamine B salt, a Rhodamine 6G salt and a Eriochrome® Cyanine R salt; the straw also comprises a gas and liquid permeable barrier buffer extending between a first end facing the first end of the tube and a second end facing the second end of the tube, with the second end of the swelling pad and the first end of the barrier pad being disposed against each other, said swelling pad and said plug forming barrier pad extending between the first end of the swelling pad and the second end of the barrier pad; the barrier buffer is a braid formed by the combination of yarns and the swelling buffer is a braid formed by the combination of yarns, with the barrier buffer comprising more yarns than the swelling buffer; the barrier buffer is formed by the combination of the yarns each comprising fibers and a coating to make the yarn hydrophobic; and / or - said coating to make the hydrophobic yarn comprises a fluororesin.
    [0009] The description of the invention will now be continued by the description of exemplary embodiments, given below by way of illustration and without limitation, with reference to the appended drawings, in which: FIG. 1 is a diagrammatic view in longitudinal section; a straw according to the invention, in the empty state; - Figure 2 is a view similar to Figure 1 but showing the straw in the filled state; - Figure 3 is a view similar to Figure 2, but showing the straw after the dose of substance that was packaged was expelled; FIG. 4 is a view similar to FIG. 3, but showing the state of the plug when it is partially out of the tube in a predetermined manner; - Figure 5 is a schematic sectional view of the swelling buffer that includes the plug of the straw; - Figure 6 is a schematic sectional view of one of the son that form the swelling buffer; - Figure 7 is a schematic sectional view of the barrier buffer that includes the plug of the straw; - Figure 8 is a schematic sectional view of one of the son that form the barrier buffer; - Figures 9 to 12 are views similar to Figures 1, 2, 5 and 7 for a first variant of the straw according to the invention; and - Figures 13 to 14 are views similar to Figures 2 and 6 for a second variant of the straw according to the invention. The straw 10 illustrated in FIG. 1 comprises a tube 11 and a plug 12. The tube 11 is conventionally made of extruded plastic material, here transparent, with an internal diameter which is here of the order of 1.6 mm and a length of the order of 133 mm. The outer diameter of the tube 11 is of the order of 2 mm.
    [0010] The tube 11 extends between one end 13 and one end 14. The plug 12 is formed by a swelling pad 15 and a barrier pad 16. The swelling pad 15 extends between an end 17 facing the end 13 of the tube 11 and an end 18 facing the end 14 of the tube 11.
    [0011] The barrier buffer 16 extends between an end 19 facing the end 13 of the tube 11 and an end 20 facing the end 14 of the tube 11. The end 18 of the swelling buffer 15 and the end 19 of the barrier buffer 16 are arranged against each other. The plug 12 extends between the end 17 of the swelling pad 15 at the end of the barrier pad 16. As will be described later in more detail, the swelling pad 15 includes a fibrous carrier and a swelling agent. associated with the fibrous support agent, which swelling agent swells by absorption of water in contact with a liquid containing it, whereby the swelling pad 15 is gas-permeable and liquid-tight. Note that the swelling pad 15 is able to play the same role as the conventional tripartite cap, but that the manufacture of the straw is simpler and more convenient since it suffices to insert the swelling pad 15 in the tube 11 (and not a first fibrous buffer, then the gelling powder and then a second fibrous buffer). The barrier buffer 16 is fibrous. It is permeable to gases and liquids.
    [0012] In the initial state, illustrated in FIG. 1, the plug 12 is disposed in the vicinity of the end 13 of the tube 11 and it is expected that, in the filled state, the dose of substance 22 (FIG. it is stored in the straw 10 is disposed between the plug 12 and the end 14 of the tube 11 farthest from the plug 12. The substance 22 is a liquid base containing water.
    [0013] To fill the straw 10, the end 13 is placed in communication with a source of vacuum while the end 14 is placed in communication with a container containing the substance 22 to be introduced into the straw. The air initially contained between the plug 12 and the end 14 is sucked through the plug 12 while the substance 22 progresses in the tube 11 until it meets the plug 12, by the end 20 of the plug barrier 16 facing the end 14 of the tube 11, that is to say by the end of the plug 12 that is seen on the right in Figures 1 and 2.
    [0014] The substance 22 passes through the barrier buffer 16 and meets the swelling buffer 15 at its end 18 facing the end 14 of the tube 11, that is to say by the end that is seen on the right in FIGS. and 2. In contact with the substance 22, a zone 23 of the swelling buffer 15 located near its end 18 assumes a state of swelling which is thwarted by the tube 11 which blocks the passage of the substance 22. The straw 10 is then exposed to filled state shown in Figure 2. It will be observed that the annoyingly swollen zone 23 of the buffer 15 is relatively short from the end 18, here of the order of 3 mm.
    [0015] It is in fact that the swelling dynamics of the swelling pad 15 in contact with the liquid-based substance 22 is such that a state of upset swelling sufficient to block the passage of the liquid is reached while swelling has occurred. only a relatively small distance from the end 18, here of the order of 3 mm.
    [0016] Although surprising, the use of a high dynamic swelling agent, i.e. capable of very rapidly absorbing a large amount of liquid, does not cause the swelling pad to absorb a large amount of the liquid-based substance 22, but on the contrary, given the speed with which the upset swelling state is reached to block the passage of the liquid, the amount of liquid absorbed is relatively moderate, for example the 3% of the dose of substance 22 introduced into the straw 10. It will be noted that the state of swelling thwarted by the tube 11 that the swelling buffer 15 provides maintains in position the swelling buffer 15 relative to the tube 11 of straw 10 which is relatively high. In particular, the buffer 15 can be maintained during handling of the straw 10 in the filled state and remain in place in the tube 11 during the freezing of the substance 22. Where appropriate, after filling, the straw is welded in the vicinity of one or both ends 13 and 14 and is stored cold. To empty the straw 10, if necessary after cutting the welded end portions and thawing, is introduced into the tube 11 a rod 25 (Figure 3) which bears against the end 17 of the swelling buffer 15 is that is to say against the end of the plug 12 that is seen on the left in FIGS. 1 to 3. With this rod, the plug 12 is slid in the manner of a piston towards the end 14, which causes the expulsion of the dose of substance 22 which had been introduced into the straw. The end 14 is at the initial location or is recessed from the original location if the tube 11 had been welded and the welded portion was cut prior to the expulsion of the substance dose 22.
    [0017] Figure 3 shows the straw 10 at the end of the expulsion of the dose of substance 22. The end 20 of the barrier buffer 16, which here forms the end of the plug 12 facing the end 14 of the tube 11, is at the end 14. If one continues to push with the rod 25 on the stopper 12, the barrier buffer 16 leaves the tube 11 and the inflating buffer 15 in turn leaves the tube 11. In the configuration shown on Figure 4, a portion 26 of the swelling pad 15 has been removed from the tube 11. Here, the length I of the portion 26 is of the order of 2 mm.
    [0018] Providing that the portion 26 of the inflated buffer 15 of the tube 11 has a length I of between 2 and 3 mm, it is ensured that the portion 26 is wholly in the annoyingly swelling zone 23, which has a length of 1 mm. order of 3 mm. As soon as it leaves the tube 11, the part 26 is decompressed. Decompacting occurs because the tube 11 no longer resumes the tension born because the swelling has been thwarted. Due to unpacking, the end 18 takes a generally convex shape and the lateral surface of the portion 26 takes a generally frustoconical shape. This is how Part 26 flourishes. Once the development completed, the end 18 has an outline which here has a diameter d of the order of 4 mm. Note that the portion 26 of the swelling buffer 15 is described above and is shown in Figure 4 very schematically. Because the portion 26 of the swelling pad 15 is decompacted, its actual contour has irregularities around the general outline described and illustrated. In practice, the configuration shown in Figure 4 can be obtained by placing the straw 10, after it has been emptied as shown in Figure 3, on a horizontal surface and pushing the plug 12 until the swelling pad 15 is taken out of the tube 11 along the length I from the end 18. To facilitate the measurement of the length I, the horizontal surface is for example formed by a sheet of graph paper. The placement of the straw 10 on the horizontal surface does not influence or has little influence on the shape taken by the portion 26, since it is after the exit of the tube 11 that the portion 26 is decompressed. As already indicated, in the uncompressed state taken by the portion 26, the swelling agent readily leaves the support agent. If, from the configuration shown in FIG. 4, the flake 10 is rolled on the surface on which it rests, the swelling agent is deposited on the surface by rolling on the surface of the part 26 The barrier pad 16 is useful in the straw so that the moistened swelling agent remains in the swelling pad 15: the barrier pad 16 prevents its passage to the substance 22.
    [0019] Note that when the barrier pad 16 is in the tube 11, it is slightly compressed and therefore relaxes slightly when it is out of the tube 11 as shown in FIG. 4. Here, the diameter of the barrier buffer 16 out of the tube 11 is a few hundredths of a mm more than the internal diameter of the tube 11.
    [0020] The swelling pad 15 in support of FIGS. 5 and 6 will now be described in detail. The swelling pad 15 is a braid formed by the combination of threads 32 (FIG. 6). Here, the swelling pad 15 is formed by nineteen identical threads arranged in a core 30 and a cover 31 surrounding the core 30.
    [0021] The core 30 is formed by three son arranged parallel against each other.
    [0022] The cover 31 is annular section. It consists of sixteen braided threads divided into eight spindles, each with two threads. One of the son 32 which form the swelling pad 15 is shown in Figure 6, in section, very schematically.
    [0023] The yarn 32 comprises support fibers 33 and swelling fibers 34. The assembly of the support fibers 33 and swelling fibers 34 to give the yarn 32 is done in a well-known manner by spinning with a twist. Here, the support fibers 33 are discontinuous filaments of polyester and / or viscose neither cracked nor carded; and the swelling fibers 34 are discontinuous filaments of sodium polyacrylate neither cracked nor carded. Sodium polyacrylate is a superabsorbent polymer (SAP) capable of absorbing several hundred times its volume of water. It should be noted that sodium polyacrylate is not spermicidal and is therefore suitable for contact with animal semen.
    [0024] The swelling fibers 34 here have a length which is at most 6 MM. The support fibers 33 are relatively aerated. This allows them to be gas permeable. In addition, the aerated nature of the support fibers 33 causes the yarn 32 to have a fluffy contour, which is favorable for maintaining the swelling pad 15 in the tube 11 when the straw is in the empty state (swelling buffer 15 in the state dry). The support fibers 33 occupy in the wire 32 a relatively large volume relative to the volume occupied by the fibers 34, which are relatively compact.
    [0025] This arrangement is favorable to the speed of liquid absorption by the threads 32: the airy nature of the support fibers 33 and the large volume occupied by the support fibers 33 allow each thread 32 to be wetted by a large amount of liquid and thus to supply liquid swelling fibers very rapidly with liquid 34.
    [0026] In the swelling pad 15 formed by the combination of yarns 32 arranged as already indicated (core 30 and cover 31), the fibrous support agent is formed by the support fibers 33 of the yarns 32 and the swelling agent is formed by the swelling fibers 34 of the threads 32. As already indicated, if, from the configuration shown in FIG. 4, the flake 10 is rolled on the surface on which it rests, swelling agent is deposited on the surface of the Part 26 is rolled over the latter. Found in the swelling agent deposited on the surface are rod-shaped elements which are swelling fibers 34 having absorbed a large quantity of liquid.
    [0027] Here, by weight, the yarn 32, in the dry state, comprises 75% of support fibers 33 and 25% of swelling fibers 34. Therefore, the swelling buffer 15, in the dry state, comprises 75% of agent fibrous support and 25% swelling agent. It is understood that for the swelling pad to remain dry, the humidity of the ambient air must remain below 50%. It will be observed that the proportion of 25% swelling agent is relatively low. Although surprising, it has been determined that the swelling dynamics of the swelling pad 15 is better (swelling faster) than with a much higher weight ratio, such as 45%. This undoubtedly stems from the fact that with a higher proportion by weight there is a smaller exchange surface so that the liquid takes longer to reach the swelling agent. In general, it has been determined that the swelling pad provides good swelling dynamics when the proportion of swelling agent is between 20% and 30% by weight. In practice, it is possible to know the swelling agent content in the swelling buffer by weighing it in the dry state (as delivered in an empty straw) and then placing the swelling buffer. in a water-permeable envelope so that the yarns remain grouped, then leaching the assembly to remove the swelling agent (which is quasi-liquid in the wetted state and which is thus eliminated by leaching) then by weighing the yarns remaining in the dry state, which then comprise the only fibrous support agent. Here, the swelling buffer has a dry weight of the order of 1.07 mg per mm of length.
    [0028] In general, it has been determined that the swelling pad 15 provides very good performance when it has a weight of between 0.8 and 1.2 mg per mm of length. The barrier buffer 16 will now be described in detail in support of FIGS. 7 and 8.
    [0029] The barrier buffer 16 is a braid which is here formed by thirty-two identical threads arranged in a core 35 and a cover 36 surrounding the core 35. The core 35 is formed of two son arranged parallel to each other .
    [0030] The cover 36 is annular section. It is formed of thirty braided threads divided into six spindles each comprising two threads and six spindles each comprising three threads. One of the son 37 which form the barrier buffer 16 is shown in Figure 8, in section, very schematically.
    [0031] The wire 37 is formed of fibers 38 similar to the support fibers 33 of the wire 32 and by a coating 39 making the wire 37 hydrophobic. Here, the coating 39 is fluororesin. Since the yarns 37 are rendered hydrophobic by the coating 39, the barrier pad 16 has a repellent effect on the water.
    [0032] This repellent effect does not prevent the substance 22 from crossing the barrier buffer 16 and reaching the swelling buffer 15, since in practice the substance 22 comes to meet the plug 12 with a certain speed. During the passage of the substance 22 in the barrier buffer 16, the son 37 do not absorb liquid; and after the contraction-swollen zone 23 of the swelling pad 15 is formed and the passage of the liquid-based substance is blocked, the barrier pad 16 does not hold the liquid in its interstices but forces it back into the dose of liquid substance located between the end 20 of the barrier buffer 16 and the end 14 of the tube 11. Therefore, there is no consumption or very little consumption of liquid substance by the barrier buffer 16.
    [0033] The variant of the straw 10 shown in FIGS. 9 and 10 is similar to the straw 10 which has just been described in support of FIGS. 1 to 8, except that: the swelling buffer 15 is shorter , here with a length (distance between its ends 17 and 18) which is of the order of 3 mm; the tube 11 has an internal diameter which is larger, here of the order of 2.5 mm; and - similarly, the swelling pad 15 and the barrier pad 16 have a larger diameter. The outer diameter of the tube 11 is of the order of 3 mm.
    [0034] Here, the swelling pad 15 of the straw 10 shown in FIGS. 9 and 10 is made with the same threads 32, but in greater numbers, than the swelling buffer 15 of the straw 10 shown in FIGS. 1 to 4. More precisely , as shown in FIG. 11, the swelling buffer 15 of the straw 10 shown in FIGS. 9 and 10 is here a braid formed by forty-eight identical threads arranged in a core 30 formed of sixteen braided threads divided into twelve spindles each comprising two son and a cover 31, annular section and surrounding the core 30, formed of twenty-four braided son divided into twelve spindles each having two son. Here, the barrier pad 16 is made with yarns 37 that are similar but thicker than the yarns 37 of the barrier pad 16 of the straw 10 shown in FIGS. 1 to 4. More precisely, as shown in FIG. 12, the barrier pad 16 of the straw 10 shown in Figures 9 and 10 is here a braid formed by twenty-eight identical son arranged in a core 35 formed of four son arranged parallel to each other and a cover 36, annular section and surrounding the l soul 35, formed of twenty-four son divided into twelve spindles each comprising two son.
    [0035] Because the swelling pad 15 has a length (distance between its ends 17 and 18) which is of the order of 3 mm, the zone 23 with contraction swelling, which is also here of the order of 3 mm, s' extends, as seen in FIG. 10, over the entire length of the swelling pad 15.
    [0036] Part of the inflating buffer 15 output tube 11 over a length of about 2 mm from the end 18, blooms like the portion 26 shown in Figure 4. Once the completion completed, the end 18 has an outline which here has a diameter d of the order of 6 mm.
    [0037] In general, by providing that the portion of the inflating pad output from the tube 11 has a length I of between 2 and 3 mm, it is ensured that the portion of the tube 11 entirely belonged to the annoyingly swollen zone 23. which has a length of the order of 3 mm. To observe the expansion, a portion 15 of the swelling pad 15 should be retained in the tube 11. For example, with the pad 15 of the straw 10 shown in FIGS. 1 to 4, the exit portion of the tube may have a length I of 3 mm since the swelling pad 15 is longer. With the swelling pad 15 of the straw 10 shown in Figures 9 and 10, the exit portion of the tube should have a length smaller than 3 mm (this is the length of the swelling pad 15). Note that the braid arrangement of the son 32 which form the swelling pad 15 allows the son 32 to hold each other but that in the vicinity of the ends the son 32 can easily be distressed and deviate from each other. other.
    [0038] The portion of the inflating pad 15 leaving the tube, both for the straw 10 shown in FIGS. 1 to 4 and for the straw 10 shown in FIGS. 9 and 10, is close to the end 18. The braiding of the wires 32 prevents the threads 32, or more precisely what remains after swelling of the swelling fibers 34, to deviate from each other.
    [0039] In practice, in general, the end such as 18 of the part such as 23, of length between 2 mm and 3 mm from the end such as 18, blooms with the end such that 18 which takes a diameter that can go up to three times the inner diameter of the tube. In general, it has been determined that the straw as it offers very good performance when the portion such as 23 of the swelling pad such as 15, of length between 2 mm and 3 mm from the end such 18, flourishes with the end such that 18 which takes a diameter at least equal to one and a half times the internal diameter of the tube. For example, for the straw 10 shown in Figures 1 to 4, whose inner diameter is of the order of 1.6 mm, the diameter of the end 18 is at least equal to 2.4 mm; and for the straw 10 shown in Figures 9 and 10, whose inner diameter is of the order of 2.5 mm, the diameter of the end 18 is at least equal to 3.7 mm. The swelling of the swelling agent is then adequately countered by the tube 11.
    [0040] By having its swelling thus frustrated by the tube 11, the swelling agent of the swelling pad 15 becomes so compact that it blocks the passage of the liquid-based substance 22 while it confers a mechanical resistance to the swelling zone 23 upset. In particular, the swelling pad 15 remains coherent (the swelling agent and the fibrous support agent are held together) when the swelling pad 15 is slid into the tube 11 to empty the straw. note that the blocking of the liquid-based substance 22 provided by the swelling pad 15, which occurs particularly rapidly as explained above, is particularly suitable for filling the straws 10 with an automatic machine, whose rate can to be very high, up to several thousand glitter per hour. In the straw 10 illustrated in FIGS. 9 and 10, the fact that the cap is shorter maximizes the space available for the storage of the liquid-based substance.
    [0041] It will be observed that in the straw 10 illustrated in FIGS. 1 to 4, the length of the plug 12, that is to say the distance between the end 17 of the swelling buffer 15 and the end 20 of the barrier buffer 16 is the order of 10 mm, and that the distance between the end 14 of the tube 11 and the end 17 of the swelling buffer 15 is of the order of 7 mm. The straw 10 illustrated in Figures 1 to 4 can be used exactly as a conventional straw. In particular, to be emptied, it can be arranged in a conventional insemination gun. It will be observed that in the flake 10 illustrated in FIGS. 1 to 4, whose inside diameter of the tube is of the order of 1.6 mm, and whose internal section of the tube is therefore of the order of 2 mm 2, the swelling pad 15 has nineteen threads 32 or 9.5 threads per mm 2 section of the tube; and that in the straw 10 illustrated in FIGS. 9 and 10, whose inner diameter of the tube is of the order of 2.5 mm, and whose inner section of the tube is therefore of the order of 5 mm 2, the buffer It has forty-eight threads or 9.6 threads per mm2 of section of the tube. In general, it has been determined that the swelling pad 15 provides very good performance when there is between 8 and 11 threads per mm 2 of section of the straw tube. In general, with the exception of the coating 39 of the threads 37, the barrier pad 16 of the straw 10 shown in FIGS. 1 to 4 and the barrier pad 16 of the straw 10 shown in FIGS. 9 and 10 are configured as one of the fibrous buffers of a conventional tripartite stopper.
    [0042] Alternatively, the barrier buffer 16 is replaced by another similar barrier buffer, whose son 37 does not include the coating 39 making them hydrophobic. In this case, the barrier buffer 16 has no repulsive effect on the liquid.
    [0043] Once the straw is filled, the swelling pad 15 continues to block the passage of the liquid-based substance 22 in an appropriate manner for several hours, i.e., the usual time between filling and freezing straw or its direct use for artificial insemination.
    [0044] It is understood that the blockage of the passage of the liquid-based substance 22 by the swelling buffer 15 is neither absolute nor permanent.
    [0045] For example, if the flake 10 is left in the filled state at room temperature beyond a few hours, i.e. outside the usual conditions of use of a straw, a small amount of The liquid-based substance is able to propagate in the swelling pad 15 beyond the annoyingly swollen zone 23, probably by capillarity along the threads 32. When the straw is frozen, it is no longer useful to block the passage substance 22, which is then in the solid state. The flake variant 10 shown in Fig. 13 is similar to the flake 10 shown in Figs. 1 to 4, except that the threads 32 which form the inflating pad 15, shown in Fig. 14, are similar to the flap 10 shown in Figs. wire 32 shown in Figure 6 but have a coating 40 to change color between the dry state and the wet state. In the empty state, the swelling pad 15 of the straw 10 shown in Fig. 13 has the same appearance as the swelling pad 15 of the straw 10 shown in Fig. 1. In the filled state, the area 23 with upset swelling The pad 15 of the straw shown in Fig. 13 has a different appearance of the pad 15 when dry. More specifically, when the flake 10 shown in Figs. 13 is in the empty state, the swelling pad 15 has a first color and when this straw 10 is in the filled state shown in Fig. 13, the moistened portion of the swelling pad 15 (zone 23 with upset swelling) has a second color. Here, the hue of the first color (empty state) is slightly brownish white while the hue of the second color (filled state) is slightly greenish yellow.
    [0046] For example, the swelling pad 15 when the straw is in the empty state, as seen through the tube 11, has a Pantone® 155U color and the area 23 of the swelling pad 15 when the straw is in the filled state ( Figure 13), as seen through the tube 11, has a Pantone® 395C color. We recall here that the hue of a color corresponds to the wavelengths (or the unique wavelength if the color is part of the rainbow) of the light emitted by the object having this color. . Hue is just one of the components of color, which depends on other parameters such as brightness and saturation. The change in color of the swelling pad 15 between the dry state and the wetted state is due to the presence of the color change coating 40.
    [0047] Here, the coating 40 is sodium salt fluorescein. It should be noted that the sodium salt of fluorescein is not spermicidal and is therefore suitable for contact with animal semen. It is known that the sodium salt of fluorescein has the following formula: Na0 'tep'0 and that it is identified by the CAS number 518-47-8. It is also known that the sodium salt of fluorescein is a fluorofore salt, that is to say capable of emitting fluorescent light, when it is dissolved in water; while in the dry state it is a non-fluoroforic salt. When the swelling buffer 15 of the straw 10 shown in FIG. 13 is in the dry state, the sodium salt of fluorescein does not emit fluorescent light since it is in a dry state. When this swelling buffer is in the moistened state, the fluorescein sodium salt is dissolved in the water contained in the portion 33 and then emits fluorescent light. The change in color of the portion 23 of the swelling buffer 15 is due to the addition of the fluorescence light. Due to the presence of the sodium salt of fluorescein, the swelling pad 15 forms a component indicative of contact between the plug 12 and the substance 22: the swelling pad 15 has a first predetermined color in the absence of prior contact with the substance 22 and a second predetermined color, having a hue different from the hue of the first color, when the swelling pad 15 has been in contact with the substance 22. The substance-contacting indicator component 22 that forms the swelling pad 15 is useful for check the correct filling of the flake ONa 10, and more precisely the good moistening of the plug 12 by the substance 22. The control of the correct filling of the straw can be done visually by the operator, by simple verification that the swelling pad 15 plug 12 has taken the shade of the second predetermined color, that is, a slightly greenish yellow tint in the present example. The control of the correct filling of the flake 10 can also be carried out automatically. In a variant of the swelling buffer 15, the coating 40 in sodium salt of fluorescein in the dry state is replaced by another non-fluorophore product in the dry state and fluorophore when it is dissolved in water, which is in the form a salt in the dry state. It is for example another salt of fluorescein, a salt of Rhodamine B, a salt of Rhodamine 6G and / or a salt of Eriochrome® Cyanine R. It is known that Rhodamine B has the following formula: and that it is identified by CAS 81-88-9. Rhodamine 6G is known to have the following formula: H3C and is identified by CAS No. 989-38-8. Eriochrome ® Cyanine R is known to have the following formula: and is identified by CAS RN 64-18-9. By choosing one or more of these products, the hue of the swelling pad 15 can be adjusted to the moist state.
    [0048] In other embodiments, the agent such as swelling buffer-modifying coating 40 is colorant, but not fluorophore. The color-modifying agent being a colorant, without being a fluorophore, is, for example, methylene blue or α-zurine. Such coloring products, when in the dry state, do not influence or influence much of the color of the other products forming the swelling pad 15. On the other hand, when the swelling pad 15 is moistened, the In other embodiments, the color change of the swelling pad 15 is effected by contact with a liquid other than water, for example a product contained in a diluent. or a seed preservative for animal semen. In other variants, the material of the tube such as 11 is not transparent, but translucent, for example slightly colored. In variants not shown, the threads 32 of the swelling buffer 15 are different from the threads shown in FIGS. 6 and 14, with for example the integration of the swelling agent which is carried out by coating fibers such as fibers support 33, for example by dipping yarns made of such support fibers or by hot coating, or by coextrusion of multi-filament support fibers and a monofilament swelling fiber, or by intimate mixing of multi-filament support fibers. with a monofilament swelling fiber.
    [0049] In other variants not shown, the swelling pad 15 has different lengths, for example longer or shorter than the swelling pad 15 of the straw 10 shown in FIGS. 1 to 4. In other variants not shown, the plug 12 is formed only by the swelling pad 15 (there is no barrier buffer 16). In other, non-illustrated embodiments, the yarns such as 32 forming the swelling pad as are associated other than by braiding, for example by twisting; and / or the support fibers 33 are of a material other than polyester and / or viscose, for example polyamide or polypropylene.
    [0050] Many other variants are possible depending on circumstances, and it is recalled in this regard that the invention is not limited to the examples described and shown.
    权利要求:
    Claims (15)
    [0001]
    REVENDICATIONS1. A straw for maintaining a predetermined dose of liquid-containing substance (22) containing water, including a biological substance, comprising a tube (11) extending between a first end (13) and a second end (14) ) and comprising a gas-permeable and liquid-tight swelling pad (15), which swelling pad (15) is disposed in the tube (11) adjacent its first end (13) and extends between a first end (17) ) facing the first end (13) of the tube (11) and a second end (18) facing the second end (14) of the tube (11), which swelling plug (15) comprises a fibrous support agent and an agent swelling agent associated with the carrier, which swelling agent swells by absorption of water in contact with the liquid-based substance (22), with said swelling buffer (15) and said tube (11) which are configured so that after the liquid-based substance (22) has come to when the inflating pad (15) meets its second end (18), the inflating pad (15) blocks the passage of the liquid-based substance (22) and is pushed on its first end (17) and is tube (11) to the second end (14) of the tube (11); characterized in that said swelling pad (15) is configured to be, at least near its second end (18), after the liquid-based substance (22) has encountered its second end (18), in a state of contraction swelling by said tube (11) such that if a first portion (26) of the swelling pad (15) of length between 2 mm and 3 mm from the second end (18) of the swelling pad (15) is removed from the tube (11) through the second end (14) of the tube (11), while a second portion of the swelling pad (15) remains in the tube (11), the first portion (26) of the swelling pad (15) is decompacts and blooms with the second end (18) of the swelling pad (15) which takes a diameter (d) at least equal to one and a half times the inner diameter of the tube (11).
    [0002]
    2. straw according to claim 1, characterized in that the swelling pad (15) is a braid formed by the combination of son (32).
    [0003]
    3. straw according to claim 2, characterized in that the swelling pad (15) comprises between eight and eleven son per mm2 section of the tube (11) of the straw (10).
    [0004]
    4. straw according to any one of claims 1 to 3, characterized in that the swelling buffer (15) has a weight of between 0.8 and 1.2 mg per mm of length.
    [0005]
    5. straw according to any one of claims 1 to 4, characterized in that the swelling buffer (15) comprises a proportion by weight of swelling agent of between 20% and 30%.
    [0006]
    6. straw according to any one of claims 1 to 5, characterized in that the swelling agent is a superabsorbent polymer configured to absorb several hundred times its volume in water.
    [0007]
    7. straw according to any one of claims 1 to 6, characterized in that the swelling agent is sodium polyacrylate.
    [0008]
    8. straw according to any one of claims 1 to 8, characterized in that said swelling pad (15) is formed by the combination of son each comprising support fibers (33) and swelling fibers (34), said agent carrier being formed by the carrier fibers (33) and said swelling agent being formed by the swelling fibers (34).
    [0009]
    9. straw according to any one of claims 1 to 8, characterized in that the swelling pad (15) has a first predetermined color in the absence of prior contact with the substance (22) and a second predetermined liquid color , having a hue different from the hue of the first color, when in contact with said substance (22).
    [0010]
    10. Flake according to claim 9, characterized in that said swelling buffer (15) comprises a non-fluorophoric salt in the dry state and fluorophore when it is dissolved in water.
    [0011]
    11. Flake according to claim 10, characterized in that said salt is part of the group comprising a fluorescein salt, a salt of Rhodamine B, a salt of Rhodamine 6G and a salt of Eriochrome® Cyanine R.
    [0012]
    12. straw according to any one of claims 1 to 11, characterized in that it further comprises a barrier pad (16) permeable to gases and liquids extending between a first end (19) facing the first end; (13) of the tube (11) and a second end (20) facing the second end (14) of the tube (11), with the second end (18) of the swelling pad (15) and the first end (19) of the barrier pad (16) which are arranged against each other, said swelling pad (15) and said barrier pad (16) forming a plug (12) extending between the first end (17) of the swelling pad (15). ) and the second end (20) of the barrier buffer (16).
    [0013]
    13. Sequins according to claim 12, characterized in that the barrier pad (16) is a braid formed by the combination of threads (37) and the inflating pad (15) is a braid formed by the combination of threads (32). ), with the barrier buffer (16) having more threads than the swelling buffer (15).
    [0014]
    14. Straw according to any one of claims 12 or 13, characterized in that the barrier buffer (16) is formed by the combination of son (37) each comprising fibers (38) and a coating (39) to make the hydrophobic thread (37).
    [0015]
    15. Sequins according to claim 14, characterized in that said coating (39) to make the thread (37) hydrophobic comprises a fluororesin.
    类似技术:
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    同族专利:
    公开号 | 公开日
    CN104891001B|2018-10-12|
    EP2910217B1|2019-12-11|
    US9918464B2|2018-03-20|
    CA2882391C|2022-01-04|
    US20150237848A1|2015-08-27|
    FR3017793B1|2019-08-23|
    JP2015178488A|2015-10-08|
    CN104891001A|2015-09-09|
    EP2910217A1|2015-08-26|
    CA2882391A1|2015-08-21|
    DK2910217T3|2020-03-16|
    JP6476004B2|2019-02-27|
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    法律状态:
    2016-02-19| PLFP| Fee payment|Year of fee payment: 3 |
    2017-02-24| PLFP| Fee payment|Year of fee payment: 4 |
    2018-02-26| PLFP| Fee payment|Year of fee payment: 5 |
    2019-02-26| PLFP| Fee payment|Year of fee payment: 6 |
    2020-02-27| PLFP| Fee payment|Year of fee payment: 7 |
    2021-02-24| PLFP| Fee payment|Year of fee payment: 8 |
    2022-02-25| PLFP| Fee payment|Year of fee payment: 9 |
    优先权:
    申请号 | 申请日 | 专利标题
    FR1451418A|FR3017793B1|2014-02-21|2014-02-21|PAILLETTE FOR PRESERVING A PREDETERMINED DOSE OF A LIQUID-BASED SUBSTANCE, IN PARTICULAR A BIOLOGICAL SUBSTANCE|
    FR1451418|2014-02-21|FR1451418A| FR3017793B1|2014-02-21|2014-02-21|PAILLETTE FOR PRESERVING A PREDETERMINED DOSE OF A LIQUID-BASED SUBSTANCE, IN PARTICULAR A BIOLOGICAL SUBSTANCE|
    CN201510190504.9A| CN104891001B|2014-02-21|2015-02-17|The tubule of the substance of liquid base for preserving setting dosage, especially biological substance|
    CA2882391A| CA2882391C|2014-02-21|2015-02-18|Straw for storing a predetermined dose of a liquid substance, in particular a biological substance|
    US14/627,405| US9918464B2|2014-02-21|2015-02-20|Straw for the storage of a predetermined dose of a liquid-based substance, in particular a biological substance|
    EP15155871.5A| EP2910217B1|2014-02-21|2015-02-20|Straw for storing a predetermined dose of a liquid substance, in particular a biological substance|
    DK15155871.5T| DK2910217T3|2014-02-21|2015-02-20|Straw for storing a predetermined dose of a liquid substance, especially a biological substance|
    JP2015032877A| JP6476004B2|2014-02-21|2015-02-23|Straw for storing a certain amount of liquid-based material, especially biological material|
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