• 专利附录
  • 专利说明
  • 权利要求
  • 类似技术
  • 同族专利
  • 引用文献
  • 法律状态
  • 优先权
    • 专利摘要:
    Microencapsulation procedure of oils in microorganisms, product obtained by that procedure and uses thereof. The present invention relates to a microencapsulation process of oils in microorganisms selected from: microalgae or cyanobacteria, the product obtained from that process and the uses given to said product. (Machine-translation by Google Translate, not legally binding)
    公开号:ES2728088A1
    申请号:ES201830383
    申请日:2018-04-19
    公开日:2019-10-22
    发明作者:Bañares Víctor Casado
    申请人:Neoalgae Micro Seaweeds Products S L；
    IPC主号:
    专利说明:

    [0001]
    [0002] MICROENCAPSULATION PROCEDURE OF OILS IN MICROORGANISMS, PRODUCT OBTAINED BY THAT PROCEDURE AND USES OF THE SAME
    [0003]
    [0004] The present invention relates to a microencapsulation process of oils in microorganisms selected from: microalgae or cyanobacteria, the product obtained from that process and the uses given to said product.
    [0005]
    [0006] Background of the invention
    [0007]
    [0008] Microalgae are used in food and cosmetics generally in powder form, this format facilitates their conservation, encapsulation, distribution and addition in different formulas. It is known that microalgae have bioactive compounds of commercial interest, however, it is interesting to incorporate other ingredients in the same formula without modifying their physical characteristics such as, for example: moisture or its powdered form.
    [0009]
    [0010] Patent application with application number WO2016059262 describes a method of enriching microalgae with polyunsaturated fatty acids. This procedure does not present any stage of rupture of the outer membranes of the microalgae and to achieve good results it is necessary to stabilize the oil in the living cell using emulsifiers or ethanol.
    [0011]
    [0012] From what is known in the state of the art, new procedures are necessary that stabilize active ingredients in microorganisms selected from microalgae and cyanobacteria.
    [0013]
    [0014] Description of the invention
    [0015]
    [0016] The present invention relates to a process that covers an oil with microorganisms selected from: microalgae or cyanobacteria.
    [0017]
    [0018] An advantage of the present invention is that it does not use any emulsifier or gelling agent so that the oil is incorporated into the microorganisms. In addition, an additional oil coating ingredient that is intended to be used is not necessary because they are the microorganisms: microalgae or cyanobacteria, which act as a coating.
    [0019]
    [0020] With this process, microorganisms can be enriched with oils from sources other than those of microorganisms, to provide them with added value without ceasing to be an easily manageable solid.
    [0021]
    [0022] The oil is incorporated into the microorganisms so that they encompass the oil and it is not lost during processing. In the process of the invention, the cellular structure of the microorganism selected from microalgae and / or cyanobacteria is modified to give rise to a heterogeneous matrix with the embedded oil.
    [0023]
    [0024] Therefore, a first aspect of the invention relates to an oil microencapsulation process in microorganisms selected from: microalgae or cyanobacteria comprising the steps of:
    [0025] a) add an oil to microalgae or cyanobacteria;
    [0026] b) break the cell envelope: cell wall and / or plasma membrane of the microorganism;
    [0027] c) dehydrate the product obtained in step b).
    [0028]
    [0029] In the present invention the term "microencapsulation" refers to coating an oil with a microorganism envelope, which is resistant and stable and immiscible with the oil, although adherent to it and which is only altered by releasing its content in certain media such as example of the intestinal tract itself.
    [0030]
    [0031] In the present description the term "microalgae" refers to a photosynthetic unicellular eukaryotic microoganism that lives in both fresh and salt waters, which convert water and sunlight into carbon dioxide and biomass.
    [0032]
    [0033] In the present description the term "cyanobacteria" refers to a photosynthetic unicellular prokaryotic microoganism that lives in both fresh and salt waters, which convert water and sunlight into carbon dioxide and biomass.
    [0034]
    [0035] A second aspect of the description refers to a product obtained by the process of the invention.
    [0036] Another aspect of the invention relates to the use of the product of the invention as a food supplement, a pharmaceutical composition and / or a cosmetic composition.
    [0037]
    [0038] Finally, another aspect of the invention relates to a capsule comprising the product of the invention.
    [0039]
    [0040] Detailed description of the invention
    [0041]
    [0042] As stated above, the first aspect of the invention refers to a method of incorporating oil into microorganisms selected from: microalgae or cyanobacteria comprising the steps of:
    [0043] a) add oil to microalgae or cyanobacteria;
    [0044] b) break the cell envelope: cell wall and / or plasma membrane of the microorganism;
    [0045] c) dehydrate the product obtained in step b).
    [0046]
    [0047] Advantageously, since the microorganisms are not alive, the conditions of the environment where the microorganisms are found are not very demanding and can be very variable. Preferably the microorganisms are in an aqueous medium. Therefore, preferably in step a) oil is added to microalgae or cyanobacteria in a medium, particularly in an aqueous medium.
    [0048]
    [0049] Preferably in the process of the invention the addition of step a) is carried out in the presence of water. When stage a) is carried out in water the concentration of the microorganism is between 1% and 500% w / w and the oil concentration is between 1% and 500% w / w with respect to the weight of the microorganism . More preferably step a) is carried out in water the concentration of the microorganism is between 1% and 10% w / w and the oil concentration is between 1% and 10% w / w with respect to the weight of the microorganism.
    [0050]
    [0051] Preferably step b) of rupture of the cell envelope is performed by emulsion by stirring the microorganisms with the oil. Preferably in the process of the invention the microorganisms are not alive. Since the stirring times in the emulsion stage in the present invention are not alive, they are very short, preferably between 5 minutes and 70 minutes, more preferably between 5 and 20 minutes; It is also not necessary to incorporate a light source that Keep microorganisms alive. Preferably, the emulsion stage is carried out in a temperature range between 20 ° and 100 ° C. More preferably the temperature range is between 25 ° C and 30 ° C.
    [0052]
    [0053] As stated before, the present procedure is carried out without emulsifiers. It is also carried out without oil solvents. The oil is added directly without adding an emulsifier, the oil is emulsified by stirring and by the components of the microorganisms themselves. It is the process itself that allows the inclusion of oil in the microorganism.
    [0054]
    [0055] More preferably step b) of rupture of the cell envelope is carried out by emulsion by stirring the microorganisms with the oil and homogenizing the result of the emulsion by pressure, by ultrasound, freezing, with pH modification by acids or bases, or with osmolarity modification.
    [0056]
    [0057] Preferably, the homogenization stage is carried out under pressure in a preferred range between 1 bar and 5000 bar. More preferably it is performed in a range between 500 bars and 2000 bars.
    [0058]
    [0059] Preferably, the homogenization step is carried out by ultrasound. More preferably in a range between 1 Khz to 100 Khz and at a power in a range between 10 W to 2000 W for 20 minutes to 120 minutes. More preferably in a range between 40 Khz to 60 Khz and at a power in a range between 800 W to 1200 W for 40 minutes to 80 minutes.
    [0060]
    [0061] Preferably, the homogenization step is carried out by freezing. More preferably in a range between -1 ° C and -100 ° C. Preferably between 2 and 4 cycles are performed.
    [0062]
    [0063] Preferably, the homogenization step is carried out by modifying the pH with acids or bases. More preferably with sulfuric acid, hydrochloric acid and other acids or with bases such as sodium hydroxide, potassium hydroxide and other bases.
    [0064]
    [0065] Preferably, the homogenization step is carried out by modifying the osmolarity, preferably with a saline buffer. More preferably with 1 to 100 mM sodium phosphate buffer.
    [0066] Preferably, step c) of dehydration is carried out by lyophilization, spray drying, vacuum drying or in the oven.
    [0067]
    [0068] Preferably after step c) the product of step c) is milled to form a powder product.
    [0069]
    [0070] One of the advantages of obtaining the powder product with the incorporated oil is that liquid oils require a technique to form more complicated capsules than dry powder capsules.
    [0071]
    [0072] Preferably, the microalgae of the genus Porphyridium, Chlorella, Tetraselmis and Nannochloropsis are preferred .
    [0073]
    [0074] Preferably, cyanobacteria of the genus Arthrospira are preferred .
    [0075]
    [0076] When this description refers to oil, it refers to vegetable oils, fruit oils, plant oils, fish oils, or algae or microalgae oils. Said oils comprise fatty acids, preferably: omega-3, DHA and EPA fatty acids; vitamins, phospholipids, phytosterols, amino acids, carotenoids such as lycopene, lutein, astaxanthin and other antioxidants. Preferably, the oil is an oil extracted from Haematococcus pluvialis with a percentage in astaxanthin between 3% and 10% and the oil extracted from Schizochytrium rich in DHA. Preferably, the oil is a vegetable oil of chia and flax rich in omega3.
    [0077] Preferably, the product refers to a powder product that is obtained by the process comprising after stage c) the grinding stage of the product of stage c). Preferably, the invention relates to a capsule comprising the powder described.
    [0078]
    [0079] Preferably, the product contains oil in a range between 15% w / w and 30% w / w oil. More preferably between 20% w / w and 25% w / w.
    [0080]
    [0081] Preferably, the invention relates to the use of the product of the invention as a food supplement.
    [0082] EXAMPLES
    [0083]
    [0084] Example 1
    [0085] 800 grams of Arthrospira platensis in 6000 milliliters of distilled water at room temperature was incorporated into a stirred reactor. After 20 minutes 200 ml of Haematococcus pluvialis oil was introduced while stirring until an emulsion was achieved. This mixture was kept under stirring while it was passed through a homogenizer at 1500 bar and collected in trays. The trays were then frozen at -40 ° C and dried by lyophilization.
    [0086] Once the product was dried, it was ground and the powder generated in capsules was incorporated.
    [0087]
    [0088] Example 2
    [0089] 1000 grams of Arthrospira platensis in 10,000 milliliters of distilled water at room temperature. After 20 minutes 100 ml of Haematococcus pluvialis oil was introduced . This mixture was kept under stirring while posing as a 500 bar homogenizer and collected in trays. They were then spray dried.
    [0090] Once the product was dried, it was ground and the powder generated in capsules was incorporated.
    [0091]
    [0092] Example 3
    [0093] 1000 grams of Arthrospira platensis in 10,000 milliliters of distilled water at room temperature. After 20 minutes, 100 ml of Haematococcus pluvialis oil was introduced. This mixture was kept under stirring and collected in trays. The trays were then frozen at -40 ° C and dried by lyophilization.
    [0094] Once the product was dried, it was ground and the powder generated in capsules was incorporated.
    [0095]
    [0096] Example 4
    [0097] 1000 grams of Arthrospira platensis in 3000 milliliters of distilled water at room temperature. After 60 minutes, 300 ml of Haematococcus pluvialis oil was introduced. This mixture was kept under stirring while being passed through a homogenizer at 1500 bar and collected in trays. The trays were then frozen at -40 ° C and dried by lyophilization.
    [0098] Once the product was dried, it was ground and the powder generated in capsules was incorporated
    [0099]
    [0100] Example 5
    [0101] 1000 grams of Arthrospira platensis in 10,000 milliliters of distilled water at room temperature. After 100 minutes 100 ml of Haematococcus pluvialis oil was introduced . This mixture was frozen at -40 ° C and thawed at 4 ° C and collected in trays. The trays were then frozen at -40 ° C and dried by lyophilization.
    [0102] Once the product was dried, it was ground and the powder generated in capsules was incorporated
    [0103]
    [0104] Example 6
    [0105] 1000 grams of Arthrospira platensis in 10,000 milliliters of distilled water at room temperature. After 100 minutes 100 ml of Haematococcus pluvialis oil was introduced . This mixture was kept under stirring while ultrasound was applied at 50 Khz and 700W for 0 minutes and collected in trays. The trays were then frozen at -40 ° C and dried by lyophilization.
    [0106] Once the product was dried, it was ground and the powder generated in capsules was incorporated
    权利要求:
    Claims (10)
    [1]
    1. Oil microencapsulation procedure in microorganisms selected from: microalgae or cyanobacteria comprising the steps of:
    a) add an oil to microalgae or cyanobacteria;
    b) break the cell envelope: cell wall and / or plasma membrane of the microorganism;
    c) dehydrate the product obtained in step b).
    [2]
    2. Method according to claim 1 characterized in that in step a) the oil is added to the microalgae or cyanobacteria in an aqueous medium.
    [3]
    3. Method according to any of claims 1 to 2 characterized in that step b) of rupture of the cell envelope is performed by emulsion by agitating the microorganisms with the oil.
    [4]
    4. Method according to any of claims 1 to 2, characterized in that step b) of rupture of the cell envelope is carried out by emulsion by stirring the microorganisms with the oil and homogenizing the result of the emulsion by pressure, by ultrasound, freezing, with pH modification by acids or bases, or with osmolarity modification.
    [5]
    5. Method according to any of claims 1 to 4, characterized in that step c) of dehydration is carried out by lyophilization, spray drying, vacuum drying or in the oven.
    [6]
    Method according to any one of claims 1 to 5, characterized in that after step c) the product of stage c) is milled to form a powder product.
    [7]
    7. Product obtained by the procedure defined in claims 1 to 6.
    [8]
    8. Product obtained by the method of claim 6.
    [9]
    9. Capsule comprising the powder defined in claim 8.
    [10]
    10. Use of the product according to any of claims 7 to 9 as a food supplement.
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    同族专利:
    公开号 | 公开日
    ES2728088B2|2021-03-02|
    引用文献:
    公开号 | 申请日 | 公开日 | 申请人 | 专利标题
    US20060233845A1|2002-02-28|2006-10-19|Gerold Lukowski|Micro/nanoparticle obtained from lipid-containing marine organisms for use in pharmaceutics and cosmetics|
    US20100303989A1|2008-10-14|2010-12-02|Solazyme, Inc.|Microalgal Flour|
    WO2016059262A1|2014-10-15|2016-04-21|Universidade De Santiago De Compostela|Method for the enrichment of microalgae biomass in polyunsaturated fatty acids|
    US20180000137A1|2015-01-26|2018-01-04|Roquette Freres|Method for preparing a flour of lipid-rich crushed microalgae|EP3935958A1|2020-07-08|2022-01-12|Neoalgae Micro Seaweeds Products, S.L.|Encapsulated oil|
    法律状态:
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    优先权:
    申请号 | 申请日 | 专利标题
    ES201830383A|ES2728088B2|2018-04-19|2018-04-19|PROCEDURE FOR MICROENCAPSULATION OF OILS IN MICROORGANISMS, PRODUCT OBTAINED BY THIS PROCEDURE AND USES OF THE SAME|ES201830383A| ES2728088B2|2018-04-19|2018-04-19|PROCEDURE FOR MICROENCAPSULATION OF OILS IN MICROORGANISMS, PRODUCT OBTAINED BY THIS PROCEDURE AND USES OF THE SAME|
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