• 专利附录
  • 专利说明
  • 权利要求
  • 类似技术
  • 同族专利
  • 引用文献
  • 法律状态
  • 优先权
    • 专利摘要:
    Fattening additive in animal nutritional composition. The invention relates to the use of at least 10 ppm and up to 500 ppm based on the total berberine composition obtained from natural or synthetic origin of formula (I), where berberine is in the form of a salt selected from berberine hydrochloride or Berberine sulfate present at a concentration of at least 95% by weight with respect to the total weight of the berberine composition, as a fattening additive in an animal nutritional composition. (Machine-translation by Google Translate, not legally binding)
    公开号:ES2681489A1
    申请号:ES201730297
    申请日:2017-03-06
    公开日:2018-09-13
    发明作者:Santiago DOLZ LUNA
    申请人:Vall Garraf S L；Vall Garraf SL；
    IPC主号:
    专利说明:

    The present invention relates to the use of berberine as an ingredient or additive in nutritionanimal. In particular, it refers to the use of berberine in the form of a salt selected fromBerberine hydrochloride or berberine sulfate as an ingredient or additive in animal feed orDrink intended to be ingested by the animal. 10 Background of the invention
    At present, diarrhea in animal farms has become a prevalent disease, which can even cause the death of the animal, resulting in a great economic loss for farmers.
    15 Thus, the provision of antibiotics for the control and prevention of diseases caused by bacteria and / or protozoa is common in animal production. Current products to control bacteria and protozoa are mostly antibiotics or substances that can end up being harmful to the environment, such as zinc oxide.
    20 In addition, due to undue administration in the long term, a large number of bacteria have become resistant to medications, so that the drugs commonly used are less and less effective, even in some cases they are 100% resistant, representing the Control of these diseases every time a major difficulty. There is
    25 increasing pressure to achieve a decrease in antibiotic use due to possible cross-resistance that may appear in human medicine, see Timothy
    F. Landers et al. in "A Review of Antibiotic Use in Food animals: Perspective, Policy, and Potential."
    30 The use of berberine in humans has been described as antidiarrheal, antiparasitic, analgesic, anti-inflammatory and anti-carcinogenic.
    Berberine in humans has also been used in topical pharmaceutical formulations in EP2448577 for the treatment of rosacea and other skin disorders related to the red face in humans. 2


    As an alternative to antibiotics, in the animal sector, various groups of products have been proposed that try to help work with a lower load of antibiotics, for example, organic acids, probiotics, prebiotics, enzymes, immune system stimulants, oils
    5 essentials, extracts from different plants, or mixtures of these components.
    Although trials on the effectiveness of berberine in animals such as antidiarrheal and antiprotozoal have been reported in the literature, berberine has been described in general in the pharmaceutical field.
    Description of the invention
    Due to the high demand for food of animal origin, it is desirable to increase its production, so many efforts have been made to find mechanisms that
    15 accelerate animal growth, especially during its first life periods where it is more vulnerable to external factors.
    The present invention is directed to the use of at least 10 ppm and up to 500 ppm based on the total berberine composition obtained from plant or synthetic origin of formula:
    25 where the berberine is in the form of a salt selected from berberine hydrochloride or berberine sulfate, present at a concentration of at least 95% by weight with respect to the total weight of the berberine composition, as a natural additive or breeding ingredient, fattening
    or reproduction in an animal nutritional composition.
    30 The use of berberine as a natural additive for breeding, fattening and reproduction in an animal nutritional composition is remarkable, where the animal nutritional composition includes animal feed or drink intended to be ingested by the animal. Berberine can be obtained from plant or synthetic origin.


    Berberine is a quaternary ammonium salt of the isoquinoline alkaloid group. It is found in the plants of the Berberis family, for example, Berberis aquifolium, Berberis vulgaris, among others. Its formula is C20H18NO4 + and its molar mass is 336.3612g / mol.
    5 In particular, Berberis vulgaris is a species of bush-bearing vegetable belongingto the berberidaceae family. It is known to contain isoquinoline alcoloids such asBerberine, palmatine and jatrorrhizine and alkaloids derived from thebisbenzyltetrahydroisoquinoline such as berbamine, oxiacantin and isotetrandin.
    10 Therefore, there are different sources from which to obtain berberine either in the form of an extract or parts of the plant directly without extracting or even berberine obtained synthetically.
    The berberine that is used in the present invention comes in the form of hydrochloride
    15 berberine or berberine sulfate, and the concentration of berberine in the total berberine composition is at least 95%, preferably at least 97%, by weight with respect to the weight of the total berberine composition.
    Schematically, berberine hydrochloride can be obtained from plant sources from
    20 of the general stages that follow: prepare the berberis vulgaris plant or parts thereof; smash; subject to aqueous extraction or alcoholic extraction; crystallization; dissolution; filtration; dried up sieving and packaging to obtain the berberine composition comprising solid state berberine hydrochloride.
    In one embodiment, the use is at least 50 ppm and up to 300 ppm based on the total berberine composition with respect to the weight of the animal nutritional composition.
    In another embodiment, the use is at least 100 ppm and up to 250 ppm based on the total berberine composition with respect to the weight of the animal nutritional composition.
    In the present invention, the term "animal nutritional composition" means animal feed or beverage intended to be ingested by the animal.
    In the present invention the term "animal" means an animal intended for the production of meat, milk or eggs for human consumption or intended for reproduction


    to produce animals for the previous purpose. In particular, pig, rabbit, poultry that are intended for human consumption.
    In one embodiment, berberine in the form of a salt selected from hydrochloride of5 berberine or berberine sulfate is used as a natural additive or breeding ingredient, fattening
    or reproduction in animal feed, where animal feed is a preparation that also comprises a mixture of crushed hazelnut or almond shell and citrus-based bioflavonoids.
    In another embodiment, berberine in the form of a salt selected from berberine hydrochloride or berberine sulfate is used as a natural additive or breeding ingredient, fattening
    or reproduction in beverage intended to be ingested by the animal, where said beverage is water which further comprises an organic solvent acceptable for animal consumption.
    15 The use of berberine as an additive or natural ingredient for breeding, fattening or reproduction in an animal nutritional composition is carried out by oral administration.
    In another embodiment, the animal nutritional composition is a nutraceutical formulation. 20 Brief description of the figures
    To better understand how much has been exposed, some drawings are attached in which, schematically and only by way of non-limiting example, a practical case of realization is represented.
    25 Figure 1 shows a bar chart of the daily weight gain (g) of piglets from weaning (28 days) to bait entry (+23 days) treated with zinc oxide (OZn) or with two doses of the total composition of berberine under commercial production conditions, where dose I comprises 100 ppm and dose II comprises 200 ppm.
    30 Figure 2 shows a bar chart of the daily feed intake (g) of piglets from weaning (28 days) to entry into bait (+23 days) treated with zinc oxide (OZn) or with two doses of the total composition of berberine under commercial production conditions, where dose I comprises 100 ppm and dose II comprises 200 ppm.
    35 5


    Figure 3 shows a bar chart of the daily weight gain (g) of piglets from weaning (28 days) to 14 days post weaning fed with feed containing two doses of the total berberine composition under experimental production conditions, where the dose I comprises 100 ppm and dose II comprises 200 ppm. In the bar that
    5 represents the reference control group only contains animal feed.
    Figure 4 shows a bar chart of the daily weight gain (g) of piglets 14 days post-weaning until entry into bait 36 days post-weaning fed with two doses of the total berberine composition under experimental production conditions, where the
    10 dose I comprises 50 ppm and dose II comprises 100 ppm. In the bar that represents the reference control group only contains animal feed.
    Figure 5 shows a bar chart of the daily consumption (g) of feed in piglets from weaning (28 days) to 14 days after weaning fed with feed containing two
    15 different doses of the total berberine composition under experimental production conditions, where dose I comprises 100 ppm and dose II comprises 200 ppm. In the bar that represents the reference control group, the animal is fed with untreated feed.
    Figure 6 shows a bar chart of the daily consumption (g) of feed in piglets 14
    20 days post-weaning until entry into bait 36 days post-weaning fed with feed containing two doses of the total berberine composition under experimental production conditions, where dose I comprises 50 ppm and dose II comprises 100 ppm. In the bar that represents the reference control group only contains animal feed.
    Figure 7 shows a bar chart of the average result of the weight increase (g) of piglets of the global period fed with feed containing two doses of the total berberine composition with respect to the negative control under experimental production conditions, where dose I it comprises 100ppm in the first period (figure 3) and 50 ppm in the second period (figure 4), and dose II comprises 200 ppm in the first period (figure 3) and 100 ppm
    30 in the second period (figure 4). Description of a preferred embodiment
    Two tests have been performed in order to verify the effectiveness of the use of berberine in
    35 animal feed, more specifically in piglets. 6


    1) First test: Commercial conditions (AgroTest)
    300 weaned piglets were used with 28 days and 3 treatments: Control (with 3000 ppm
    5 zinc oxide), and two groups with two different doses of berberis vulgaris extracts (total berberine composition> 97% by weight of berberine hydrochloride, dose I: 100 ppm and dose II: 200 ppm) not containing zinc oxide . 5 repetitions were performed per treatment. Data were collected on the appearance of diarrhea, consumption, weights from weaning to the entry into bait (from day 28 to 51 of life).
    10 Results In no repetition of any treatment there were diarrhea or mortality, so it can be concluded that the inclusion of berberine hydrochloride was able to keep piglets without enteric problems during the most critical period of production and the protection that
    15 provided against diarrhea was the same as that provided by zinc oxide.
    Surprisingly, it was observed that both the intake, and the growth of animals that consumed berberine hydrochloride, were much higher than those belonging to the group with zinc oxide (control). This greater growth difference, close to 30%, meant that
    20 piglets would bait with 3.3 kg more live weight than their brothers in the control group.
    2) Second test: Experimental conditions (IRTA) 144 weaned piglets were used with 26 days, 3 treatments: negative control (without any medication or additive), two phases (0-14 days and 14-36 days after weaning) with two dose
    25 different extracts of berberis vulgaris (total berberine composition> 97% by weight of berberine hydrochloride, dose I: 100 ppm and dose II: 200 ppm in phase 0-14 days and dose I: 50 ppm and dose II 100 ppm in the phase 14-36 days). 9 repetitions were performed per treatment. The experiment was carried out during the transition period until bait, and two feeds were used: Prestarter up to 14 days and start until bait.
    30 Results There were no incidents due to diarrhea or mortality in any of the 3 groups. The growth results indicate that during the first phase (pre-starter) the animals that consumed berberine hydrochloride had a growth much higher than the negative control, of the order
    35 of 18% more, and during the second phase (starter), the growth improvement was 6%


    Regarding the negative control. In the most critical phase (Prestarter), animals that had been treated with berberine hydrochloride more easily exceeded this period, growing much more than untreated animals. Once this more critical phase was overcome, during the start-up, although differences continued to be observed, these were not as notable. In
    In total, the animals that consumed berberine hydrochloride went into bait with 1 kg more live weight.
    Of both experiments, it should be noted that the best results of the inclusion of berberine hydrochloride have been observed under commercial conditions, first
    10 test, where it is very likely that the infection pressure is higher than in a research institute, second test, and perhaps this fact may explain the differences observed in daily growth when commercial production is compared with experimental farm.
    Although reference has been made to a specific embodiment of the invention, it is apparent to one skilled in the art that the use of berberine as a natural additive or ingredient for animal breeding, fattening or reproduction in a described animal nutritional composition is susceptible of variations and modifications, and that all the mentioned details can be replaced by other technically equivalent ones, without departing from the scope of protection
    20 defined by the appended claims.

    权利要求:
    Claims (6)
    [1]
    1. Use of at least 10 ppm and up to 500 ppm based on the total berberine composition obtained from natural or synthetic origin of formula:
    10 where the berberine is salt selected from berberine hydrochloride or berberine sulfate present at a concentration of at least 95% by weight with respect to the total weight of the berberine composition, as additive or ingredient for breeding, fattening or reproduction in a composition Animal nutrition
    [2]
    2. Use according to claim 1, wherein the berberine in the form of berberine hydrochloride
    15 or berberine sulfate is present at a concentration of at least 97% by weight with respect to the total weight of the berberine composition.
    [3]
    3. Use according to any one of claims 1 or 2, wherein it is at least 50 ppm and up to 300 ppm based on the total berberine composition with respect to the weight of the animal nutritional composition.
    Use according to any one of claims 1 to 3, wherein it is at least 100 ppm and up to 250 ppm based on the total berberine composition with respect to the weight of the animal nutritional composition.
    [5]
    5. Use according to any one of the preceding claims, wherein the composition
    Animal nutrition includes animal feed or drink intended to be ingested by the animal.
    [6]
    6. Use according to claim 5, wherein the animal feed is a preparation which further comprises a mixture of crushed hazelnut or almond shell and citrus-based bioflavonoids.
    [7]
    7. Use according to claim 5, wherein the beverage intended to be ingested by the animal
    30 is water which further comprises an organic solvent acceptable for animal consumption.

    FIG 2

    FIG 4

    FIG 6
    类似技术:
    公开号 | 公开日 | 专利标题
    Durrani et al.2007|Effect of different levels of feed added black seed |
    Gh et al.2011|The effects of Echinacea purpurea L.| as an antibiotic growth promoter substitution on performance, carcass characteristics and humoral immune response in broiler chickens
    ES2307179T3|2008-11-16|I THINK IT CONTAINS ALACALOID OR ADDITIVE FOR FEED.
    Khan et al.2009|Effect of fenugreek | seed extract on visceral organs of broiler chicks
    CN101357223A|2009-02-04|Preparation method of anti-stress agent for fishing
    ES2536249T3|2015-05-21|Feed additive for animals with antimicrobial effect and performance promoter
    ES2233706T3|2005-06-16|USE OF BENZOFENANTRIDINE ALCALOIDS AS ADDITIVES FOR FEED.
    CN104543363A|2015-04-29|Natural plant antibacterial growth-promoting feed additive and application thereof
    ES2732480T3|2019-11-22|Use of an extract of Scutellaria baicalensis comprising baicalin during the period of delivery to stimulate lactation in farm animals
    ES2681489B1|2019-07-09|GRINDING ADDITIVE IN ANIMAL NUTRITIONAL COMPOSITION
    ES2545866T3|2015-09-16|Compositions of abscisic acid for animal feed
    KR101208741B1|2012-12-05|A transportation method for aquareculture fish.
    JP2020162587A|2020-10-08|Feed for aquatic animals, growth improving agents for aquatic animals, and methods for rearing cultured fishes using the same feed and agents
    RU2005104894A|2006-08-10|METHOD FOR INCREASING THE MEAT PRODUCTIVITY OF YOUNG PIG ON FAT
    EP3398448A1|2018-11-07|Fattening additive for animal nutritional composition
    Akinrotimi et al.2013|Effects of clove seed as anaesthetic agents in two species of grey mullets Liza falcipinnis and Liza grandisquamis
    WO2014014334A1|2014-01-23|Animal feed supplement
    CN103283958B|2014-07-02|Formulation and preparation method of Chinese herbal feed additive for livestock and poultry
    Yadav et al.2017|Effect of aloe vera | supplementation on production indices, mortality and cost of production of broiler chicken
    Tangalin2011|Anthelmintic effects of processed mature betel nut as dewormer to native chicken and small ruminants |
    CN102715359B|2013-12-18|Natural rare bird feed addictive
    ES2819860T3|2021-04-19|Animal feed additive containing diurnoside and / or cestrumoside
    WO1998042204A1|1998-10-01|Infectious disease preventive/remedy for fishes and shellfishes
    RU2524664C1|2014-07-27|Method of prevention of mass gastrointestinal and respiratory diseases of suckling pigs
    ES2826424T3|2021-05-18|Food supplement for use against stress and anxiety in pets
    同族专利:
    公开号 | 公开日
    ES2681489B1|2019-07-09|
    引用文献:
    公开号 | 申请日 | 公开日 | 申请人 | 专利标题
    CN103356833A|2013-06-28|2013-10-23|镇江天和生物技术有限公司|Compound preparation used for controlling nutritional diarrhea of weaned piglet|
    CN103446312A|2013-08-27|2013-12-18|西安乐道生物科技有限公司|Compound poplar flower oral liquid and preparation method thereof|
    CN103549222A|2013-11-22|2014-02-05|安徽科技学院|Compound feed additive for preventing piglet diarrhea and preparation method thereof|
    法律状态:
    2018-09-13| BA2A| Patent application published|Ref document number: 2681489 Country of ref document: ES Kind code of ref document: A1 Effective date: 20180913 |
    2019-07-09| FG2A| Definitive protection|Ref document number: 2681489 Country of ref document: ES Kind code of ref document: B1 Effective date: 20190709 |
    优先权:
    申请号 | 申请日 | 专利标题
    ES201730297A|ES2681489B1|2017-03-06|2017-03-06|GRINDING ADDITIVE IN ANIMAL NUTRITIONAL COMPOSITION|ES201730297A| ES2681489B1|2017-03-06|2017-03-06|GRINDING ADDITIVE IN ANIMAL NUTRITIONAL COMPOSITION|
    [返回顶部]