西班牙专利ES2668976A1 Substrate for the control of flies and other insects, their manufacturing process and use of the sub

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专利摘要:
Substrate for the control of flies and other insects, their manufacturing process and use of the substrate as an animal bed. The invention is a substrate, especially designed for use as an animal bed, for the control of insects, preferably flies, characterized in that it comprises an absorbent substrate impregnated with at least one insect growth regulator (RCI), in a determined preferential concentration, and wherein the substrate is preferably impregnated with a formulation comprising, in addition to the growth regulator, a film-forming substance, a particulate mineral material insoluble in water, an organic solvent, one or more additives and water, in the amounts specified therein. memory. Also part of the invention is the method of obtaining the substrate, by impregnating the formulation, and using the substrate as an animal bed for insect control. The use of the waste formed by the mixed substrate with manure after being used as an animal bed is also disclosed, as an organic amendment for agricultural soils through a process of stabilization and degradation by biosolarization. (Machine-translation by Google Translate, not legally binding)
公开号:ES2668976A1
申请号:ES201600885
申请日:2016-10-21
公开日:2018-05-23
发明作者:Maria Del Pilar MATEO HERRERO
申请人:Maria Del Pilar MATEO HERRERO；
IPC主号:

专利说明:

SUBSTRATE FOR THE CONTROL OF FLIES AND OTHER INSECTS, ITS MANUFACTURING PROCESS AND USE OF SUBSTRATE AS AN ANIMAL BED SECTOR OF THE TECHNIQUE
The present invention is within the Chemical sector, specifically in the area of insect control, preferably flies, at the livestock level. In particular, the present invention provides a substrate and method for the control of flies and other insects in animal beds and other environments where animals such as stables, stables, farms or zoos live.
Additionally, the waste generated by using the substrate of the invention as a bed of animals in stables, stables, farms or zoos can be transformed by a process that degrades pesticides and subsequently be used as fertilizer and organic amendment for agricultural soils. Therefore, this invention is applicable in the field of Public Health, Agriculture and Animal Health. STATE OF THE TECHNIQUE
There are different species of flies, all of them are annoying for humans and animals, and can affect their health since flies can mechanically transmit pathogenic microorganisms and parasites. In general, the life cycle of flies comprises four stages: egg, larva, pupa and adult fly. Among the different species of existing flies, the housefly (Musca domestica) and the stable fly (Stomoxys calcitrans) are the most common, prolific and the most damaging in the livestock sector.
The housefly (Musca domestica L) can multiply very quickly, which can be a serious problem in places where animals such as cattle, horses, etc. are raised or lived. Flies generally lay their eggs in decomposing organic matter such as, for example, wet manure or spilled food. Taking into account that the housefly can multiply very quickly (as an example, a pound of wet manure can lead to more than 1500 larvae), and fast (in optimal conditions its life cycle can be only 6 days ), even in small areas of animal growth a large population of flies can be generated.
The stable fly (Sfomoxys calcifrans) is a widespread pest of cattle throughout the world. It is very common in cattle farms (especially dairy and fattening), in horses and in pig stables, but they attack any warm-blooded animal (donkeys, mules, sheep, etc.) including man. They are found mostly on the legs, on the flanks and on the backs of the animals. Males and females suck blood 2 or 3 times a day, each time for about five minutes. When they are not sucking blood they usually rest outside the host, perched on the walls or objects close to the hosts (poles, trees, fences, fences). Each female lays about 500 to 1000 eggs on decomposing organic material, to be able to be of plant origin: bales of decomposing straw, silage wells, horse or cow dung mixed with the materials used in animal beds, etc. and rarely in pure excrement. His hematophagous activity is diurnal and of exophageal habits, that is, they bite outdoors, outdoors, typical in the vicinity of the stables, although flies have been described biting inside the rooms related, normally, with fasting situations .
In addition to these two species, they can be found more occasionally in the stables and livestock farms, flies of the genera Sarcophaga, Lucilia, Calliphora and Musca, especially problematic is Musca aufumnalis (face fly), in addition to other species located in certain Geographic regions such as Haemafobia irrifans, very abundant in South America.
As mentioned earlier, flies can transmit diseases and parasites between animals, as well as between animals and humans. In particular, house flies can house more than 100 different types of pathogenic organisms. There are studies that have associated them with more than 65 human and animal diseases. In addition, they can transport eggs and infectious larvae of several parasitic worms. More specifically, flies can transmit viral diseases in cattle such as bovine viral diarrhea (BVD), bovine herpesvirus (BHV-1) causing bovine infectious rhinitis (IBR) and bovine parainfluenza 3 (PI3). Bacterial diseases caused by flies include conjunctivitis, mastitis, bacterial diarrhea, typhoid fever, anthrax, vibriosis and various clostridial diseases. Flies can also transmit diseases among horses, for example, Pigeon fever or equine infectious anemia, in addition to having a relevant role in the formation of summer sores.
A plague of flies can cause serious economic problems in livestock farms. In particular, flies can lead to a reduction in milk production, interfere with the work of operators, increase the frequency of diseases in livestock and, in relation to the above, increase veterinary costs.
Similarly, pest flies can also pose a serious economic damage in the equestrian sector, as they can affect the health of both horses and stable staff. High infestations of housefly cause stress in horses that are very nervous and altered. In the case of the stable fly (Stomoxys calcitrans L.), in addition to the problems mentioned above, there may be others related to the fact that this fly species feeds on the blood of animals and humans. Therefore, a high population of this fly species can lead to weakening of animals due to blood loss. Additionally, the bite of this species of flies is quite painful and, sometimes, can lead to allergic reactions, so that a pest of this species of flies can negatively affect other industries such as, for example, tourism. In addition, Stomoxys calcitrans can transmit various protozoa to horses such as Trypanosoma evansi and Trypanosoma equinum causing hip disease or surra.
Flies are also main vectors of Habronema spp, a genus of gastrointestinal nematodes common in horses that cause a disease called cutaneous habronemasis that causes ulcers in different parts of the animal. Since they visit many different hosts, they can also act as mechanical vectors of viral and bacterial diseases, eg of the foot and mouth disease virus.
On the other hand, there are other insects that proliferate in the materials used as animal beds, since these are an excellent shelter and shelter. This is the case of the bed beetle (Alphitobius diaperinus) that is usually present in chicken farming facilities (broilers) or the presence of various species of fleas in stables and stables of sheep, goats or cattle.
Thus, in addition to the housefly, the following insect species usually have their habitat in animal environments and presence in livestock facilities.
SPECIES FEATURES
Stomoxys • Can transmitmiscellaneousprotozoatothehorseshow
calcitrans Trypanosoma evansi and Trypanosoma equinum causing hip disease or surra. • Main vectors of Habronema spp, a genus of gastrointestinal nematodes common in horses that cause a disease called cutaneous habronemasis that causes ulcers in different parts of the animal. • They can also act as mechanical vectors of viral and bacterial diseases, eg. of foot and mouth disease virus
Alphitobius diaperinus • Proliferate in materials used as animal beds, especially in chicken farming facilities (broilers)
Fleas • Commonly present in livestock environments.
To combat all these pests present in environments where animals are raised and / or live
such as, for example, in livestock facilities, stables, farms or zoos,
A combination of various control systems is currently used. Generally this
The system includes processes of sanitation of the environment aimed at reducing breeding points, that is, removal of decomposing material where flies or other insects can develop (for example, old fodder, agricultural residues, hay, manure, spilled food) and cleaning of the facilities; biological control using natural enemies of insects whose plague you want to control; and / or chemical agents.
As control agents qUlmlco are often used insecticides in the form of aerosol, cold fogging (fine drops) and spraying, devices for direct self-application to the animal (pour-on and spot-on), baits or traps. The application of insecticidal aerosols on the surfaces where insects are usually deposited, in particular flies, that is, 15 walls, posts, roofs or other structures, creates a toxic surface that allows reducing the population of insects in the environment where they are They find animals, particularly stables and / or stables. Another fairly widespread alternative is to apply the insecticidal compound in the form of a mist, that is, very small drops that remain floating in the air for a short period of time. This treatment allows to control the population of
20 flying insects such as adult flies, as they come into contact with the insecticide while flying through the area where the treatment has been applied. In both cases it is necessary to protect the water and food of the animals, as well as the animals themselves, when the insecticidal composition is applied.
It should be noted that the use of larvicidal products, mainly insect growth regulators (RCls) such as diflubenzuron, novaluron, triflumuron and ciromazine, has become a common practice in livestock facilities. However, the application in situ by spraying or sprinkling these active agents incorporated on the manure and the bed of the animals has the following disadvantages:
• Need to repeat treatments each time on new manure or on the material of the animal's bed that is replenished during cleanings;
• Continuous handling of biocidal products by the maintainers of the livestock facilities;
• Incomplete (and, therefore, non-homogeneous) coverage of the entire surface and volume of the animals' bed, since it is generally only applied to manure presence areas. Also, the beds are only treated in the surface layer, being less effective in larvicidal action;
• The existence of untreated areas allows the development of larvae of other insects that do not live in manure, such as fleas; Y
• Contribution of abundant moisture to manure and the material of the beds due to spraying, which implies greater appetite for oviposition by flies and proliferation of fungi in animal beds.
Fly larvae can also be eliminated by incorporating an insecticide into animal feed. The incorporation into the drinking water has been less satisfactory due to the irregular amounts consumed by the animals and the difficulty of maintaining the appropriate dose per animal. These methods often give rise to residue problems in animal tissues or products.
Finally, biological control methods, consisting of the use of predators and parasitoids (mites and beetles predators of eggs and larvae of flies and parasitoid hymenoptera) have their best results with dry manure. In very wet manure, predators cannot move effectively to find and devour the eggs and larvae of flies. With regard to the use of entomopathogenic fungi, their efficacy against flies has been proven, but most species are only active on adults. Some species such as Beauveria bassiana have a broad spectrum of activity (for example, that can infect many insect species), while others are more selective (for example, Metarhizium anisopliae and Paecilomyces fumosoroseus, which are more effective against the fly of the face and horn fly).
The use of conventional insecticides to control fly pests, as well as other insects, has several drawbacks. Among them, it should be noted that flies quickly generate resistance to the insecticides used, so, if these chemical agents are chosen, it is necessary to alternate or rotate between different kinds of insecticides. In addition, there are currently new regulations that require a reduction in the levels of insecticides present in food, as well as in the levels to which humans and animals are exposed. Although the methods comprising using insecticides in the form of traps, aerosols or fog allow reducing the number of adult flies, the results obtained are limited, since it is not possible to keep the number of flies at acceptable levels for a long time. Consequently, an improved method of fly control is required to prevent their development in those places where animals are raised and / or live, in particular, cattle, horses or confined wild animals.
For the control of pests of flies or other insects in environments where animals are raised and / or live, a correct choice of the material that forms the animal's bed may be helpful. Thus, for example, the use of wood chips as an absorbent substrate is preferred instead of hay or straw, since the latter absorb the urine and decompose rapidly, making the animal's bed a suitable place for the development of insects, in particular flies. To avoid this, in case of using hay or straw as an absorbent material or substrate to form the animal's bed, it is necessary to remove the material used more frequently than if wood chips are used.
The correct choice of the material or substrate that forms the bed of an animal is relevant to ensure that it remains in a comfortable and healthy environment. Among the multiple functions that this material has is the absorption of urine and gases, as well as reducing the growth of bacteria. Absorbent substrates that can be used as an animal bed are wood shavings, wood pellets, peat, hay or straw, newspaper strips or alternative materials such as rice husks, wheat by-products, etc. All these materials, once contaminated with urine, manure or any other type of waste generated by the animal, are easy to remove from the stables and / or stables, so that the animal can be kept in a healthy environment.
Currently, part of the waste generated by removing the contaminated part by feces from animal beds, particularly from stables, stables or farms, can be used as organic fertilizer in crop fields, for example, in cane cultivation fields of sugar. However, so far, these residues do not undergo any previous treatment to eliminate the bacteria that may be present in the excrements contained in the absorbent substrate, as well as the insecticides that may have been deposited in the beds after the treatment are not eliminated as an aerosol or fog or even larvicides applied directly to the ground, bed and / or manure in situ, to control the pests of flies and other insects in the environment of animals. Therefore, the direct application of these wastes as fertilizers can cause public health problems and / or environmental pollution.
The presence of pesticides in soils constitutes an environmental problem for different environmental compartments such as surface water from runoff, groundwater from leaching and air emissions from volatilization. Additionally, these substances can affect soil microorganisms, beneficial insects and generate harmful effects on plants. The majority contribution of these substances to the soil comes from the application of phytosanitary products by farmers when it comes to combating pests and diseases in agricultural crops.
The dynamics of phytosanitary products in the soil is governed by various physical, chemical and microbiological processes. In particular, adsorption / desorption processes, leaching, chemical and microbiological degradation, absorption by the plant, volatilization, rain, climatic factors (humidity and temperature), solar radiation and the organic matter content.
DESCRIPTION OF THE INVENTION The present invention provides a substrate, specially designed to be used as an animal bed, which allows controlling the biological cycle of insects, preferably flies, preventing them from entering the adult state and, consequently, allows controlling pests of these insects in the environment where animals are raised and / or live, particularly in stables, stables, farms or zoos; as well as in the places where the waste generated by these animals is stored.
More specifically, the present invention refers to a substrate for insect control, preferably flies, characterized in that it comprises an absorbent substrate impregnated with at least one insect growth regulator (Rel). This substrate is, therefore, a material treated with said growth regulator. The term "treated material or substrate" and "impregnated material or substrate" may be considered indistinct within the scope of the present specification, since the former best conforms to the names of the regulatory framework on biocides, while the latter is a term that is technically more accepted and used to refer more directly to the way in which the product is treated (impregnated) with the regulator.
Thus, in the substrate for insect control, preferably flies, described herein, one or more insect growth regulators (Rel) are distributed homogeneously on the surface of the absorbent substrate. In this way, the use of the substrate of the present invention as an absorbent material in animal beds allows to achieve a high, controlled and reproducible effectiveness in the control of insect pests, preferably flies, in facilities where animals are raised and / or live such as stables, farms, stable or zoological, thus solving the problems detected in the technical field derived from applying these active agents directly on the manure and the bed of the animals.
In the present invention, the absorbent substrate can be selected from those known as absorbent material for animal beds. Preferably, the absorbent substrate comprised in the insect control substrate, preferably flies, of the present invention (and which is impregnated with the formulation) is selected from the group consisting of wood chips ("shavings" in English), straw, wood pellets, paper, hemp, linen, crushed cardboard and a combination of the above. More preferably, the substrate is made of wood chips, in particular pine or spruce. The chips are inedible, widely available and marketed in bags and to
bulk.
The insect control substrate, preferably flies, described herein incorporates at least one insect growth regulator (Rel), also known in the sector as IGR according to its acronym in English ("Insect Growth Regulator"). Rels are natural or synthetic products, with a structure very similar to certain hormones that regulate the growth of arthropods. Its effect is to interfere with the development of the larvae, preventing individuals from completing their reproductive cycle. Because they are substances similar to those of the arthropod itself, they cannot cause resistance phenomena.
Two main types of Rels can be highlighted.
A first type is constituted by chitin synthesis inhibitors. These compounds interfere with the formation of chitin during the molting periods. Consequently, they prevent normal growth since the structure of the exoskeleton cannot be restored correctly after molting, which causes malformations. The most commonly used chitin synthesis inhibitors are diflubenzuron, triflumuron, lufenuron, hexaflumuron, hexaflumuron, novalurone, chlorfluazuron, lufenuron, teflubenzuron and ciromazine. This type of insecticide is effective mainly when applied in the first larval states (L1, L2). The majority are synthetic products, but there are some of plant origin such as plumbagin, obtained from the roots of Plumbago capensis, which inhibits chitin synthetase, and its effect on different pest lepidoptera has been proven. Among the Rels that are inhibitors of chitin synthesis, a preferred case is that of ciromazine, which belongs to the group of triazine derivatives, and which acts by interfering with the metabolism of insect chitin. Ciromazine is quite specific for diptera larvae (muscled, caliporid, mosquitoes, fleas and some beetles, among others), which can be an advantage since it does not harm many species of insects and other beneficial organisms such as parasites and predators of other plague insects. In addition, the very low toxicological profile of ciromazine is added to this fact, even being used as a feed additive in feed.
A second type of Rel consists of analogs and antagonists of the juvenile and molt hormone. These compounds modify the levels of juvenile and mute hormone, hindering the development of the insect and moving to the adult stage, so that the insect ends up dying without reproducing. Thus, juvenile hormone analogues, or juvenoids, prevent the metamorphosis of the insect because they inhibit the differentiation of adult characters and therefore prolong the larval state, affecting fertility. They can be substances produced by the insects themselves or artificial synthesis that induce molting, causing the appearance of non-viable individuals, with intermediate characteristics of larva and pupa; inhibition of feeding that leads to a decrease in reproductive potential and other physiological alterations. Additionally, juvenile hormone analogs, also called HJ analogs, can have an ovicidal effect.
Preferred HJ analogs can be selected from the group consisting of pyriproxyfen, methoprene, phenoxycarb and a combination of the foregoing.
It should be added that there is a third group of compounds whose mechanism of action is to also affect an insect hormone, specifically ecdysone or molt hormone. These compounds are considered here as one more type of RCI because they act as growth regulators, ecdysone antagonists, and are quite specific against Lepidoptera. They do not raise ecdysone contents, such as benzoylphenylureas, but act directly on tissues; that is, they force a premature synthesis of the cuticle before the insect is, due to its development, ready to move. It is an original mode of action based on a mimetic action of the molting hormone: 20-hydroxy-ecdysone. This activity totally differentiates it from the regulators and inhibitors of insect growth: it starts the shedding process when the insect is in the larval stage, L 1-L4 states, but is not physiologically prepared for it, thus causing death of the larvae in the state of double cephalic capsule. The treated larvae stop feeding quickly after ingestion, after 2-4 hours, which stops the production of damage. Its ovicidal action is low, in general, but variable with the species; In addition, the application of the product causes a decrease in the fertility of adults altering their ability to reproduce. Preferred ecdiesteroids are: tebufenocide, methoxyphenoid, halfenocide or a combination of the above.
The insect growth regulator (RCI) present in the substrate for the control of insects, preferably flies, which is described herein prevents their normal development from the eggs placed in the feces of the animals resulting from contact. intimate between substrate and animal feces deposited in beds. Preferably, of all the types discussed above that are valid for the present invention, the insect growth regulator is selected from the group consisting of pyriproxyfen, metoprene, hydroprene, diflubenzuron, triflumuron, phenoxycarb, tebufenocide, methoxyphenocide, ciromazine and a combination of the above Even more preferably, the RCI is pyriproxyfen, for its effectiveness at very low doses, low toxicity for mammals and high degradation rate in soils. Another insect growth regulator preferred for its properties and behavior in the present invention is ciromazine.
Pyriproxyfen is a regulator of the growth of broad-spectrum insects, with activity against insect pests such as housefly, mosquitoes and cockroaches. RCIs are specific to insects and have a very low toxicity to mammals. Piriproxifene is one of four Uunto pesticides with temephos, metoprene and permethrin) recommended by the World Health Organization for the treatment of drinking water against mosquito pests.
As mentioned earlier, flies and other insects usually lay their eggs in decomposing organic material such as, for example, manure deposited in animal beds in stables and / or stables. Currently, the substrate used as an animal bed in stables and / or stables, preferably wood chips, is periodically removed for replacement and stored prior to its removal and reuse as an organic fertilizer. During all this time the eggs deposited in the manure hatch and generate the annoying pests of other insects.
Against these disadvantages, with the manufacture of the treated absorbent substrate, used in the beds for animals, by impregnating the RCI, the development of fly eggs present in the manure is affected, being able to arrive, in particular when using pyriproxyfen as RCI, 100% effective in preventing the fly from passing the juvenile stages (larva and pupa), avoiding the appearance of the adult insect. As mentioned, this effect is not achieved when the active agent is applied by spraying or the like on a substrate in situ, for the reasons stated in the previous section (lack of homogeneity in the application, appearance of untreated areas, etc. ).
In preferred embodiments of the present invention, the concentration of insect growth regulator, preferably pyriproxyfen, impregnated in the absorbent substrate is between 1 mg / kg and 6000 mg / kg, amounts expressed by weight of RCI relative to the weight of the absorbent substrate. This amount is equivalent to 0.0001-0.6% by weight with respect to the final weight of the substrate, and it should be understood that these values express the concentration of the active ingredient in the absorbent substrate, which may be referred to as "loading" of RCI in the substrate. More preferably, the concentration of insect growth regulator impregnated in the absorbent substrate is between 1 mg / kg and 500 mg / kg. More preferably, the amount of Rel by weight with respect to the final weight of the substrate is 0.0015%.
It is indicated herein with respect to this range of values as well as any disclosed herein, that the minimum and maximum values described are included as part of the invention.
In preferred embodiments of the present invention, the absorbent substrate is impregnated with a formulation comprising, amounts expressed by weight relative to the total weight of the formulation:
- between 0.005 and 10% of at least one insect growth regulator (Rel);
- between O and 5% of at least one film-forming substance;
- between O and 15% of at least one water insoluble particulate mineral material;
- between O and 25% of an organic solvent;
- between 0.01 and 10% of at least additive selected from the group consisting of emulsifier, thickener, pH regulating agent, antifoam, dispersant, preservative, fungicide and a combination of the above; and - necessary amount of water to complete 100% by weight of the formulation.
In particular embodiments of the present invention, the qualitative and quantitative composition of the formulation ingredients impregnated in the absorbent substrate correspond to the formulation composition comprising the Rel used to make the substrate of the invention, although it is possible that the water content and, eventually, other volatile ingredients such as solvents, have decreased, either during the application of a possible drying stage in the method of manufacturing the substrate of the present invention, or by the action of air or ambient heat during utilization.
Preferably, at least one insect growth regulator (Rel) is comprised in the formulation in an amount by weight with respect to the total weight of the formulation comprised between 0.001% and 10%, this amount being in the most preferred case of 0.05% when Rel is pyriproxyfen. Logically, this value varies and depends on the nature of the Rel incorporated. Thus, in the also preferred case of ciromazine, the preferred concentration would be between 0.05% and 5% and would be more preferably 0.5%.
Preferably, the film-forming substance can be comprised between 0.1% and 2% by weight with respect to the total weight of the formulation. This film-forming substance included in the formulation impregnated in the substrate described herein may be, for example, vinyl acetate homopolymers, acrylic acid homopolymers, or copolymers formed by the following monomers: vinyl acetate, acrylic acid, styrene , vinyl versatate, ethylene, vinyl chloride and butadiene, among others; they can also be thermoplastic polyurethanes; natural or modified cellulose; natural or synthetic film-forming resins such as turpentine, rosin, alkyd or alkyd resins. Preferably, the film-forming substance is a vinyl copolymer.
Additionally, the formulation comprising the RCI may comprise a particulate mineral material insoluble in water, such as talc, kaolin, calcium carbonate, barite, calcium sulfate, bentonite, sepiolite, among others. Preferably, this formulation comprises calcium carbonate. Preferably, this component is present in the formulation in a percentage by weight with respect to the total weight of the formulation between 0.1% -5%
In turn, the organic solvent may preferably be comprised in a percentage by weight with respect to the total weight of the formulation of between 0.1% and 5%. The solvents that can be used preferably in the present invention belong to the groups of hydrocarbons, alcohols, esters, ethers, ketones, amines, aliphatic or aromatic amides.
In preferred embodiments of the present invention, the formulation comprising at least one RCI may additionally comprise one or more of the following additives, in% by weight of the total formulation:
emulsifying agent, preferably being a calcium salt of the alkyl acid
benzenesulfonic acid, between 0.1-10%;
- thickener, preferably being a cellulosic compound, between 0.1-2%;
- pH regulator, preferably being an acetic-acetate buffer, between 0.1
one%;
- antifoam, being preferably selected from mineral oils, among
0.1-2%;
- dispersant, preferably being a derivative of polyacrylic acid, between 0.1
5%; -conservative, preferably being an isothiazolone, between 0.1-1%; I
- fungicide, preferably tebuconazole, between 0.1-1%.
These additives can act as stabilizers of the formulation, both prior to their application by impregnation in the absorbent substrate and once it has been impregnated in the absorbent substrate, which means that these additives maintain the stability of the formulation when impregnated in the substrate
Additionally, the present patent application refers to a method for obtaining the substrate for the control of insects, preferably flies, which is described herein, characterized in that the method comprises: applying (formulation homogeneously by impregnation) on an absorbent substrate a formulation comprising amounts expressed in percentage by weight with respect to the total weight of the formulation:
- between 0.005 and 10% of at least one insect growth regulator (RCI);
- between O and 5% of at least one film-forming substance;
- between O and 15% of at least one water insoluble particulate mineral material;
- between O and 25% of an organic solvent;
- between 0.01 and 10% of at least additive selected from the group consisting of emulsifier, thickener, pH regulating agent, antifoam, dispersant, preservative, fungicide and a combination of the above; and - necessary amount of water to complete 100% by weight of the formulation.
It is evident from the above description that in the essential method a formulation such as the one described above in any of its variants is applied, and that it is impregnated as it is desired to be maintained in the final substrate.
However, in another alternative of the method, this formulation that is impregnated can be in the form of concentrated raw material, which facilitates its storage, packaging, distribution and commercialization, so that in this particular case the method may include a previous stage. of dilution in water of the concentrated formulation, to reach the dose of use, that is, to be ready for use in impregnation. In this case, the method may include a water dilution step of a concentrated formulation comprising the following components by weight of the total formulation:
- between 1 and 50% of at least one insect growth regulator (RCI);
- between O and 25% of at least one film-forming substance; - between 0 and 50% of at least one water insoluble particulate mineral material;
- between O and 95% of an organic solvent;
- between 0.01 and 10% of at least one additive selected from the group consisting of emulsifier, thickener, pH regulating agent, antifoam, dispersant, preservative, fungicide and a combination of the above, and
- water until 100% by weight of the formulation is completed, this amount being equal to zero, before applying said formulation homogeneously by impregnation on the substrate.
Thus, it is clarified here that the formulation containing the RCI and that is impregnated in the substrate is aqueous, and in some cases instead of being ready for use, it may be in concentrated format. The concentrated version, with an RCI concentration between 1% and 50%, requires dilution in water prior to use, for which a stirred tank can be used. Alternatively, the concentrated product can be dosed in a stream of water under controlled flow conditions and dilution in line for direct application and without the need for dilution in the tank.
It should be noted based on the data provided above that there is an overlap of values between the concentration range of the RCI in the ready-to-use formulation and in the concentrated formulation, specifically between 1% and 10%. This is due to the different effective dose of each of the RCls discussed here and which may be part of the invention. Thus, it is clear that an RCI with a certain weight percentage in the ready-to-use formulation will always have a higher concentration in the concentrated formulation, and in turn this concentration may be in high or low proportion with respect to that of another RCi . Thus, it can be said that between 1% and 10% by weight of RCI the formulation can be impregnated directly on the substrate or diluted, depending on the RCI it contains.
In a preferred case, the concentration of RCI in the ready-to-use formulation is between 0.005% and 1% by weight of the total formulation, including both limits, when the RCI is pyriproxyfen, and the amount of this RCI in the concentrated formulation It is preferably between 1% and 10%.
In preferred embodiments of the present invention, the formulation comprising the RCI can be applied to the absorbent substrate, preferably wood chips, in one at the following doses:
• Formulation dosage ready to use: 2-10% by weight on weight of absorbent substrate.
• Dilution of the concentrated formulation: dilution of 0.5-20% with water and subsequent application on the material in doses 2-10% on weight of absorbent substrate.
• RCI concentration in the ready-made formula: 0.005-10%, preferably between 0.01% and 2%;
• RCI concentration in the concentrated formulation: 1-50%, preferably between 5% and 25%;
• RCI concentration in the chip: 1-6000 mg / kg or 0.0001-0.6%, preferably between 1 mg / kg and 500 mg / kg.
At this point, it should be noted that if the formulation to be impregnated comprises pyriproxyfen as RCI, then in the most preferred form said RCI is contained in the formulation in a percentage by weight with respect to the weight of the formulation comprised between 0.01% and 0, 1%, being more preferably 0.05%. Likewise, preferably the application dose of said formulation on the substrate is 15 mg / kg of substrate (preferably wood chips). In the case of ciromazine, preferably its concentration in impregnating solution is 0.5% and with a load of 150 mg / kg of substrate.
The substrate impregnated with RCI described in this patent application is particularly suitable for use as a component of the beds of horses and other animals, for which it must have a low humidity level that allows its absorption function and, on the other its role in the control of immature states of flies in manure; by "low", a moisture level between 8% and 12% should be understood herein, never exceeding this value. Therefore, the amount of formulation (dose) used for impregnation is as important an parameter as the concentration in RCI (preferably pyriproxyphene or ciromazine) thereof, since at a lower dose, lower humidity of the final product.
The application of the formulation in the absorbent substrate can be carried out by one of the techniques selected within the group consisting of spraying, spraying,
fogging or contact with a surface impregnated with the formulation. Preferably, in the method of the present invention the formulation is applied by spraying.
More specifically, the impregnation of the absorbent substrate, in particular wood chips, can be carried out by various means or combinations thereof. In particular, it can be done by one of the following methods:
• Spraying the formulation comprising the RCI through diffuser nozzles with variable droplet size between 1 and 200 microns with the help of a pump;
• Nebulization of the formulation comprising the RCI by action of pressurized air
or pumping;
• Contact with applicator roller coated with the formulation comprising the RC !.
For this, the impregnation system can be based on the advance and movement of the absorbent substrate by various means or combination thereof simultaneously with the application of the formulation comprising the RCI, preferably pyriproxyfen:
• Gravity fall of the absorbent substrate through a conduit with or without the action of baffles that divide the curtain flow into two or more fractions;
• Advance by inclined plane by means of a fluidized bed that moves the absorbent substrate by pneumatic action;
• Advance by inclined vibrating table; I
• Feed by inclined trommel with rotation and turning of the absorbent substrate; This system is preferred by the suitability for the transport of the material and by the continuous movement of the same, causing its separation and change of face as a result of the turning.
In the substrate for insect control, preferably flies, which is obtained by the method described in this patent application the RCI is homogeneously distributed, so that the active ingredient is conveniently distributed on the surface of the substrate.
In the manufacturing method described herein, when the substrate impregnated with the formulation comprising the insect growth regulator has a humidity greater than 12%, the method comprises an additional step of drying the impregnated absorbent substrate to a value. maximum humidity of 12%, that is to say that it does not exceed 12%, and that preferably it is between 8% -12%.
The drying of the impregnated material (substrate) can be carried out by various means or combination thereof. In particular, one of these means or a combination of them can be used:
• Convective drying by flow of hot air from a burner or an air heating system by other means. Alternatively, air can be used at room temperature (that is, between 20 and 30 oC) if the drying needs are lower. This system is preferred for the economy and simplicity of its industrial execution as well as for the use of lower temperatures than the radiants, so that degradation of the material is prevented;
• Radiant drying by infrared burners, electrical resistors or infrared radiation.
In order to obtain a greater homogenization in the impregnation of the RCI in the absorbent substrate, preferably wood chips, one of the following systems or a combination thereof can be used:
• Suspension of the absorbent substrate by pneumatic action in a chamber with the formulation comprising the RCI sprayed in fine drops. Additionally, movement can be helped by eccentric vibration of the transport table;
• Fall from the height of the absorbent substrate through a duct with the presence of baffles to divide the flow and spray from several points of the formulation comprising at least one RCI;
• Stirring and advancing the absorbent substrate with screw screw inside a chamber and sequential spraying of the formulation along it;
• Turning of the absorbent substrate with combs on the conveyor belt surface and sequential spraying of the formulation along it;
• Turning of the absorbent substrate by rotation and advance of the material inside a cylinder. This system is considered to be preferred because of the disintegration effect and the turning frequency it achieves.
In other preferred embodiments, the absorbent substrate is wood chips, and the method of manufacturing the substrate for insect control also comprises the manufacture of these chips. In general, the wood chip manufacturing process can
comprise the following stages: a) cutting or shaving of the wooden logs, preferably pine or spruce, to form wood flakes of thicknesses between 0.5 and 2 mm; b) drying, for example in hot air dryers, to reduce the moisture content, preferably up to a maximum moisture content of 12% by weight with respect to the total weight of chips; c) purification to remove dust and fine particles; and d) packaging, for example, in sacks or in bulk.
The purification of step c) can be carried out, for example, through a cyclone and sieve. The fine particles separated at this stage can be used as fuel in biomass burners that feed the dryer in hot air in stage b) of flake drying.
Alternatively, this process of obtaining chips can be reduced to cutting wood and packaging, in the case of lower quality products.
In preferred embodiments, wood chips, preferably of pine or spruce, used to obtain the substrate for insect control, preferably flies, described in this patent application have a moisture content between 8% and 12% by weight with respect to to the total weight of chips.
In the insect control substrate, preferably flies, which is described herein, the Rel can be fixed to the chips at any stage of the chip manufacturing process, preferably after purification by sieving. In this way, only the final absorbent substrate is treated and the generation of atmospheric emissions is avoided during the combustion of impurities (dust and fine particles), which would take place if the formulation is applied to the chips before the purification stages .
The impregnation operation is preferably carried out in line with the normal production of the wood chip, inserting an impregnation unit in the process (see Figure 1), although it can also be carried out in isolation to the manufacturing process using already finished chip. The inclusion of this stage in the production line itself represents advantages in terms of process economics and simplicity of operation.
The present invention also refers to the insect control substrate, preferably flies, obtained by the manufacturing method described in this patent application. This substrate has a particularly homogeneous distribution of both Rel and other components that may be present in the impregnation formulation, so they are particularly advantageous when used to control insect pests, preferably flies, in environments where they live or animals are raised.
Additionally, the present invention refers to the use of the substrate for the control of insects, preferably flies, described herein as animal beds. In particular, for species such as horses, cattle, sheep, pigs, goats and other confined wild animals, preferably horses. Preferably, the fly control substrate of the present invention is used as an animal bed in stables and stables.
This treatment of the substrate used in animal beds prevents the normal development of flies and other insects from the eggs that are deposited in the feces of the animals present in the beds.
Additionally, the present invention relates to animal beds formed by the substrate for the control of flies and other insects described herein. Preferably, when the substrate comprises wood chips impregnated with pyriproxyfen, it is in a concentration between 1 and 100 mg / kg, and is distributed homogeneously and uniformly by the wood chips.
The present invention also refers to a method for obtaining beds for animals which comprises depositing the substrate for the control of flies and other insects described in this patent application in the place where the animal is located, preferably in stables, stables and farms In preferred embodiments of the present invention, this method also comprises the manufacture of the substrate, in particular that comprising wood chips and pyriproxyfen, as described herein.
In recent years several techniques have been developed for the decontamination of contaminated soils from the degrading effect of microorganisms on pollutants. Within these bioremediation techniques of soils contaminated by phytosanitary products, solarization and biosolarization have been highlighted, since in most cases it is possible to accelerate the degradation of these compounds in the soil (Solarization enhances dissipation of carbendazim (MBC) in soil, Yarden et al., Soil Biology and Biochemistry. 21 (1989) 857-861).
Solarization of the soil was described for the first time as a method to disinfect soils against a wide variety of fungi, nematodes, parasitic phalangogams (weeds) or arthropod pests, bacterial diseases, disease complexes, microorganisms associated with a state of weakness and other harmful agents of biotic or abiotic character. As a consequence of all this, the application of the technique of solarization in agricultural land allows to increase the harvest and improves the quality of the crops.
However, the solarization process for soil disinfection involves several problems associated, in particular, there is a temperature gradient between the treatment center (higher temperature) and the outside of the margin of the covered surface (normal temperature) that causes that, from one meter from the edge to the limit, the temperature decreases progressively. In this marginal area there is a loss of effectiveness that should be avoided. The escape of gases from the edges can also cause a lack of efficiency.
It has also been described the contribution of manure or remains of brasicas (agricultural waste) to improve the process of solarization for soil disinfection. In this technique, called biosolarization, volatile substances produced in the biodecomposition of organic matter have an effect on the control of plant pathogens. Finally, the increase in the amount of organic matter in the soil provided by biosolarized manure to agricultural soil translates into an increase in fertility, improved structure and reduction of salinity problems.
The results found show that the use of organic amendments in biosolarization allows to further accelerate the degradation processes in the soil of the insecticides pirimicarb and piroproxifen (Rate of loss of insecticides during soil solarization and soil biosolarization. José Fenoll, Encarnación Ruiz, Pilar Hellín, Carmen M. Martínez, Pilar Flores, Journal of Hazardous Materials 185 (2011) 634-638) and also several fungicides (Solarization and biosolarization enhance fungicide dissipation in the soil. José Fenoll,
Encarnación Ruiz, Pilar Hellín, Simón Navarro, Pilar Flores. Chemosphere 79 (2010) 216220).
In relation to the foregoing, the present patent application refers to a subsequent phase of biosolarization of the bed substrate (which has been described above as an object of protection) together with the manure that is removed from the stables, farms or stables. Essentially it consists of accumulating said substrate in outdoor enclosures and placing a sheet of transparent plastic to achieve that when solar radiation strikes a strong increase in the temperature inside that causes a degradation of the active ingredients, preferably pyriproxyfen, of so that the mixture of substrate and manure is perfectly usable for subsequent work of agricultural fertilization without risk to crops or soil.
This recovery process can include the prior irrigation of the waste to be treated and, subsequently, the placement of a transparent plastic to provide adequate humidity, preferably between 40 and 60%, to achieve a conduction of the optimum temperature within the material. The type of plastic is preferably a low density polyethylene sheet with a thickness of 25 to 100 microns. This sheet is preferred as a cover material, because of its price, tensile strength and radiation transparency.
This recovery process is applied in the present invention directly on the mixture of manure and absorbent substrate of interest for the control of insects in order to stabilize the organic matter, eliminate pathogens and parasites, and finally degrade the pesticides contained in this residue. The increase in temperature and the abundant concentration of microorganisms accelerate the degradation processes and their performance. In this way, the manure and substrate residue for insect control of the present invention can be transformed as an organic fertilizer or amendment, without causing public health problems and / or environmental contamination.
BRIEF DESCRIPTION OF THE FIGURES Figure 1: Scheme representing the method of manufacturing the substrate for fly control of the present invention, comprising wood chips as absorbent substrate and the method further comprises the manufacture of wood chips from the logs. This scheme is subdivided into two figures, Figure 1 a and Figure 1 b, the first one specifically showing the part of the method that includes the surface cutting of the wood and subsequent drying of the chips, as well as the subsequent purification, while the second illustrates the continuation of the process with impregnation of the formulation and subsequent treatment and packaging.
Thus, in particularly preferred embodiments of the manufacturing process of the wood chips impregnated with Rel described herein, the wood logs pass through a mill (1) where the cutting or shaving of the wood logs takes place. controlled way to obtain fine wood flakes (chips) in a controlled process; wood chips pass through the drying zone (2) and then undergo a sieving purification process through a cyclone (3) and a sieve (4). The impregnation zone (5) is located at the exit of the screen (4), this zone comprises a reservoir with the formulation comprising Rel (6), systems for applying the formulation from the reservoir to the impregnation system, where the system of impregnation can be by advance by inclined plane by means of a fluidized bed that moves the wood chips by pneumatic action (5a), spraying the formulation comprising the Rel through diffuser nozzles (5b) or advancing and turning the material by rotation of the cylinder and spray of the formulation comprising the Rel (5c). In any of the three options, the formulation comprising the Rel is supplied from a reservoir (6). Subsequently, the chips impregnated with the formulation comprising at least one insect growth regulator are taken to a packaging area (7), where they are packaged in bags of the desired size or stored in bulk, and distributed by trucks (8 ) or any other transportation system.
Figure 2: Scheme showing the use of the substrate for fly control as a horse bed, and the subsequent collection of the substrate with the excrement in the biosolarization process to valorize the animal beds. This scheme is subdivided into two figures, Figure 2a and Figure 2b. The first figure specifically shows the part of the process that includes its distribution and storage and its incorporation into the stable as an animal bed, that is, its use. Subsequently, the second figure illustrates how this bed, after use, is removed from the barn as a residue for the biosolorization treatment.
In particularly preferred embodiments of the present invention, wood chips impregnated with the Rel are distributed by trucks (8) or any other means of transport to a storage area (9) until use. In one of its preferred applications, the wood chips impregnated with RCI of the present invention are transferred to a stable (10), deposited in a manger (11) as an animal bed for a horse (12). After a certain period of time, the manger (13) is cleaned and the waste formed by the horse's droppings and the chips removed from the manger are stored in a tank (14). Subsequently, this residue is subjected to a biosolarization process (15) where both the RCI present in the chips and microorganisms that may be present in the organic matter are eliminated. Finally, the treated residue is extracted from the biosolarization tank (16) and is used as an organic fertilizer in an agricultural crop (17).
Figure 3: Graph showing the mortality results of M. domestica pupae with wood chips impregnated with the formulations detailed in Table 1 as described in test 1.
Figure 4: Graph showing the mortality results of M. domestica pupae with wood shavings impregnated with the pyriproxyfen formulations detailed in Table 3 as described in trial 2.
Figure 5: Graph showing the mortality results of M. domestica pupae with wood shavings impregnated with the pyriproxyfen formulations detailed in Table 5 as described in test 3.
Figure 6: Graph showing the mortality results of M. domestica pupae with wood shavings impregnated with the pyriproxyfen formulations detailed in Table 7 as described in trial 4.
Figure 7: Graph showing the mortality results of M. domestica pupae with wood shavings impregnated with ciromazine formulations detailed in Table 9 as described in trial 5.
EXAMPLES First, four experiences are described to quantitatively assess the effectiveness of various preparations of pine wood chips impregnated with RCls on larvae and pupae of housefly (Musca domestica, L.). The first trial served to verify the effectiveness of the substrate obtained and select pyriproxyfen as an ingredient
active (Rel), while the second one, carried out, like the first one, on rabbit manure, allowed to choose a certain type of impregnation formulation and profile the concentration and most suitable dose. The results of the second trial were confirmed in the third on horse manure. In the fourth and final test, it was used to define the optimal concentration of pyriproxyfen in the impregnation formulation (0.05% weight with respect to the weight of the formulation) and the application dose (15 mg of pyriproxyfen per kilogram of wood chips). The results obtained demonstrate the efficacy of the treatment for the products and doses tested, with pyriproxyfen being the most effective active even at very low doses.
Secondly, a trial is described to quantitatively evaluate the efficacy of various preparations of pine wood chips impregnated with ciromazine on larvae and pupae of housefly (Musca domestica, L.).
Material and methods The absorbent substrate used for impregnation was "Great Northern" pine wood shavings, which are the most frequently used in horse beds.
For efficacy studies, the application of the formulations for the impregnation of the shavings was carried out manually by means of a spray gun container. Several sprays were made with intermediate chip turns to ensure the impregnation homogeneity.
The organic material used was rabbit manure in the first two trials of pyriproxyfen, due to its greater availability and its high infestation by fly larvae. For tests 3 and 4 of pyriproxyfen and the test carried out with ciromazine, horse manure was used because it was the main object of product application. In both cases, manure with high infestation of fly larvae was selected to obtain an appropriate number of larvae per test.
For the performance of the efficacy tests, a mixture of shavings / manure in a proportion 30170 was placed in different trays, adding 50 g of shavings per 117 g of manure without making an excessive mixing of both materials that could facilitate the contact of the larvae With the treated chip. The trays were covered with a mesh to prevent the emergence of an adult.
With the intention of reproducing on a small scale the storage conditions the chips impregnated with RCI with manure in the facilities with horses, the trays were kept outdoors in natural conditions, in which periods of alternating
5 shade with periods of sunstroke.
In the case of the tests of beds with pyriproxyfen, after 5 days of exposure (enough time for the larvae to have passed into the pupa stage), the pupae were separated in plastic cups covered with a mesh and the emergency of grown ups. A
Once the adults emerged, the unviable pupae and the emerged adults (healthy and defective) were counted to calculate the percentage of mortality in each case.
In the case of the ciromazine trial, after 5 days of exposure, the pupae and dead larvae were separated. Subsequently, once the adults emerged, the dead larvae and unviable pupae and the emerged adults (healthy and defective) were counted to calculate the percentage of mortality in each case.
In all the trials there was an untreated chip control.
20 Test Results 1. Four different formulations were tested, under the conditions set out in the following table (Table 1). For this, 4 formulations were prepared:
Table 1. Fonnulations with pyriproxyfen and diflubenzuron on rabbit manure
N ° RCI concentration (weight with respect to the total weight of the phonulation)Type of FonnulationDose (formulation weight with respect to chip weight)Load (mg of active / Kg chips)
one Diflubenzuron 10%Concentrated suspension6%6000
2 1% PyriproxyfenConcentrated Suspension6%600
3 Pyriproxyfen 1% + diflubenzuron 10%Concentrated suspension6% + 6%600 + 600
4 100% DiflubenzuronProduct0.6%6000
Iteen; e,
sprinkled
I I
In addition, a control without wood chips (C) and another with untreated wood chips (1) were tested. The mortality results of M. domestica pupae with wood chips impregnated with the formulations detailed in Table 1 obtained in this first test are shown in Table 2 and Figure 3.
Table 2. Mortality results of M. domestica pupae in wood shavings with the formulations in Table 1
N ° Total individualsAdultsPupae not emerged% Mortality
C (control) 10910187.3
OR 14213664.2
one 1813. 414781, 2
2 161 215998.8
3 182one18199.5
4 2229312958.1
10 The results of this first trial were used to select pyriproxyfen as an insect growth regulator (RCI) of the formulation, since the mortality results for diflubenzuron (formulations 1 and 4) are good, but not as good as those of formulations containing pyriproxyfen (formulations 2 and 3).
15 It should be noted that the only non-liquid formulation (formulations 4) shows the worst mortality results, probably due to its lower dispersal capacity and adherence to the substrate (wood chips).
Test 2. Formulations with pyriproxyfen on rabbit manure
20 The formulations prepared for impregnation were aqueous dispersions and emulsions with different concentrations of pyriproxyfen (Table 3):
Table 3. Pyriproxyfen formulations on rabbit manure RCI Concentration (Weight Dose Type (Weight Load
one
2
3
4
5
6
with respect to the total weight of the formulation)
Pyriproxyfen 0.5%
Pyriproxyfen 0.1%
1% Pyriproxyfen
Pyriproxyfen 0.2%
Pyriproxyfen EW 0.5%
Pyriproxyfen EW 0.2% formulation
Water dispersion
Water dispersion
Water dispersion
Water dispersion
Aqueous emulsion
Aqueous emulsion formulation
respect to the
weight fromthe
shavings)
6%
6%
6%
6%
6%
6%
(mg of active / Kg chips)
300
150
600
150
300
150
As in trial 1, a control with manure without shavings (C) was performed. Mortality results on M. domestica pupae with wood shavings impregnated with the formulations with pyriproxyfen described in Table 3 in rabbit manure are shown in the following table (Table 4) and in Figure 4.
Table 4. Mortality results of M. domestica pupae in wood shavings with the pyriproxyfen formulations of Table 2 in rabbit manure
N ° Total individualsAdultsPupae not emerged% Mortality
C (control) 7162912.7
one 35 one3. 497.1
2 85one8498.8
3 42OR42100
4 132OR132100
5 112OR112100
6 71OR71100
10 The results obtained showed high efficacy for all formulations used in impregnating wood chips. The best results (100% mortality) were produced with formulations 3, 4, 5 and 6.
In view of these results, it was decided to repeat the experience impregnating chips
15 wood with the lowest concentration formulations of pyriproxyfen and horse manure.
Test 3. Trays were prepared with wood chips impregnated with 2 formulations with pyriproxyfen (see Table 5 below) and applied on horse manure.
Additionally, a tray with untreated wood chips was prepared that served as a control group (C).
Table 5. Formulations with pyriproxyfen on horse manure
N ° Pyriproxyfen concentration (weight with respect to the total weight of the formulation)Type of formulationDose (formulation weight with respect to chip weight)Load (mg of active / Kg chips)
one Pyriproxyfen 0.2%Aqueous emulsion6%120
2 Pyriproxyfen 0.1%Aqueous emulsion6%60
Mortality results on M. domestica pupae with wood chips 10 impregnated with pyriproxyfen formulations in horse manure are shown in the following table (Table 6) and Figure 5.
Table 6. Mortality results of M. domestica pupae in wood shavings with the formulations in Table 5
N ° Total individualsAdultsPupae emergedno% Mortality
C (control) 53431019
one 93OR93100
2 41OR41100
Again, 100% mortality was obtained for the two substrates prepared with the two pyriproxyfen formulations, both 0.2% and 0.1% (Table 6).
Trial 4: In view of the results, a final trial was prepared with the objective of
20 evaluate the effectiveness of the substrate impregnated with pyriproxyfen solutions at a lower concentration than those tested so far (0.05% and 0.01%), applying them in several doses (3% and 6%), in order to optimize the RCI concentration and application dose.
For this, substrate impregnated with the formulations specified in Table 7 was evaluated:
Table 7. Formulations with pyriproxyfen on horse manure
N ° Pyriproxyfen concentration (weight with respect to the total weight of the formulation)Type of formulationDose (formulation weight with respect to chip weight)Load (mg of active / Kg chips)
one Pyriproxyfen 0.1%Aqueous emulsion6%60
2 Pyriproxyfen 0.05%Aqueous emulsion6%30
3 Pyriproxyfen 0.01%Aqueous emulsion6%6
4 Pyriproxyfen 0.1%Aqueous emulsion3%30
5 Pyriproxyfen 0.05%Aqueous emulsion3%fifteen
6 Pyriproxyfen 0.2%Aqueous emulsion3%60
Formulation 5 of test 4 comprises amounts expressed in percentage by weight
regarding the total weight of the composition:Pyriproxyfen: 0.05%vinyl copolymer: 0.385%
10 additional additives: 1.72% (surfactants 0.02%, solvent 1.6%, preservative 0.1%) water: csp. 100%
The rest of the formulations tested in this study include, quantities expressed
15 in percentage by weight with respect to the total weight of the composition: Rel: as indicated in any one of the tables 1, 3, 5 or 7 vinyl copolymer: O -5% additional additives: 0.5-5% water: csp . 100%
20 Additional additives are surfactants, solvents (other than water) and preservatives that provide stability to the formulation.
Mortality results on M. domestica pupae with wood shavings impregnated with the pyriproxyfen formulations described in Table 7, in horse manure are shown in Table 8 and Figure 6.
Table 8. Mortality results of M. domestica pupae in wood shavings with the formulations of Table 7
N ° Total individualsNormal adultsPupae not emerged% Mortality
OR 3938one2.6
one 71 one7098.6
2 79OR79100
3 4424295.5
4 135 413195
5 fifty24896
6 47OR47100
The results obtained corroborate the good efficacy of pyriproxyfen as a pupicide at very low doses. Even with formulation # 3, where the amount of pyriproxyfen per kg of wood chips is the lowest (6mg / kg), the effectiveness is very good (95.5%).
Test 5. Four different formulations with ciromazine on horse manure were tested (Table 9):
Table 9. Ciromazine formulations on horse manure
N ° CompositionType of formulationDose (formulation weight with respect to chip weight)Load (mg of active / Kg chips)
one Ciromazine 1%Water dispersion6%600
2 Ciromazine 0.5%Water dispersion3%150
3 Ciromazine 0.5%Water dispersion6%300
4 Ciromazine 0.1%Water dispersion6%60
In addition, a control formulation without wood chips (C) and a control product of untreated wood chips (O) were tested.
The mortality results of M. domestica pupae with wood chips impregnated 20 with the formulations detailed in Table 9 obtained in this test are shown in Table 10 and Figure 7.
Table 10. Mortality results of M. domestica pupae in wood chips impregnated with formulations of Table 9 with ciromazine in horse manure
N ° Total individualsAdultsDead larvaePupae not emerged% Mortality
e (control) 8780one68
OR 10392245.8
one 79OR79OR100
2 128 2124 298.4
3 134one130 399.3
4 1173578 470
The results confirmed the effectiveness of the chips impregnated with ciromazine and, in addition, allowed to select the ideal concentration of the formulation to be impregnated and the recommended application dose for it.
10 Wood chips treated with formulation 1 were the most effective product (100% mortality) but formulations 2 and 3 also obtained a high mortality (98.4% and 99.3% respectively) with the fourth part and half load than formulation 1, respectively. Therefore, its cost / effectiveness is much better.
15 Wood chips impregnated with solution 4 obtained good efficacy results, but comparatively they did not reach the high mortality rates of the other formulations, indicating that the asset load per kilogram of chips was too low in this case.
20 Ultimately, this test allowed to verify the effectiveness of the final product when the Rel agent is ciromazine, becoming a valid option for the control of domestic flies in animal beds object of the present invention. It also allowed to select the concentration of the solution (0.5% of ciromazine) and dose (150mg / kg of wood chips). Formulations with very low concentrations of active
25 (ciromazine) and very low doses of the product are sufficient to exercise adequate control of the immature states of M. domestica in manure.
After the five tests performed, several conclusions can be drawn:
- The substrate impregnated with RCls has been shown as an effective tool for controlling manure flies; -The RCls used (pyriproxyfen, diflubenzuron and ciromazine) have shown efficacy at low doses, with pyriproxyphene and ciromazine the best percentages of 5 mortality has presented; - Substrates with loads of 15 mg / kg of pyriproxyfen and even 6 mg / kg, or 150 mg / kg of ciromazine have shown high efficacy in the control of housefly pupae. -In the choice of the appropriate formulation for impregnation, other factors must be taken into account in addition to its effectiveness.

权利要求:
Claims (15)
[1]
one. A substrate for insect control, characterized in that it comprises an absorbent substrate impregnated with at least one insect growth regulator.
[2]
2. The substrate for the control of insects according to claim 1, wherein the insect growth regulator is selected from the group consisting of pyriproxyfen, metoprene, hydroprene, diflubenzuron, triflumuron, phenoxycarb, tebufenocide, methoxyphenoid, ciromazine and a combination of the foregoing.
[3]
3. The substrate for insect control according to claim 2, wherein the insect growth regulator is selected from pyriproxyfen and ciromazine.
[4]
Four. The substrate for insect control according to any one of claims 1 to 3, wherein the concentration of the insect growth regulator in the absorbent substrate is between 1 mg / kg and 6000 mg / kg, by weight of growth regulator of the insect with respect to the weight of absorbent substrate.
[5]
5. The substrate for insect control according to any one of claims 1 to 4,
comprising an absorbent substrate impregnated with a formulation comprising: between 0.005 and 10% of at least one insect growth regulator; -between 0 and 5% of at least one film-forming substance; -between 0 and 15% of at least one water insoluble particulate mineral material; -between 0 and 25% of an organic solvent; -between 0.01 and 10% of at least additive selected from the group consisting of emulsifier, thickener, pH regulating agent, antifoam, dispersant, preservative, fungicide and a combination of the above; and - necessary amount of water to complete 100% by weight of the formulation.
[6]
6. The insect control substrate according to any one of claims 1 aS, wherein the absorbent substrate is selected from the group consisting of wood chips, straw, wood pellets, paper, hemp, linen, crushed cardboard and a combination of the previous.
[7]
7. A method for obtaining the substrate for insect control described in a
Any one of claims 1 to 6, characterized in that the method comprises: applying a formulation comprising an amount comprising weight percentage to the total weight of the formulation on an absorbent substrate:
-between 0.005 and 10% of at least one insect growth regulator;-between 0 and 5% of at least one film-forming substance;-between 0 and 15% of at least one water insoluble particulate mineral material;-between 0 and 25% of an organic solvent;-between 0.01 and 10% of at least additive selected from the group consisting ofemulsifier, thickener, pH regulating agent, antifoam, dispersant,preservative, fungicide and a combination of the above; Y-Necessary amount of water to complete 100% by weight of the formulation.
[8]
8. The method according to claim 7, wherein the formulation is applied by impregnation by a technique selected within the group consisting of spraying, spraying, fogging and contact with a surface impregnated with the composition.
[9]
9. The method of any one of claims 7 or 8, wherein the substrate impregnated with the formulation comprising the insect growth regulator has a humidity greater than 8% and the method comprises an additional step of drying the impregnated absorbent substrate to a value. maximum humidity of 12% if this value is exceeded after impregnation.
[10]
10. The method according to any one of claims 7 to 9, wherein the insect growth regulator is selected from pyriproxyfen and ciromazine and the amount of formulation applied is between 2 and 10% by weight relative to the final weight of absorbent substrate.
[11 ]
eleven . The method according to any one of claims 7 to 10, wherein the formulation to be applied on the substrate is in concentrated form comprising the following components by weight of the total formulation:
-between 1 and 50% of at least one insect growth regulator (Rel);-between 0 and 25% of at least one film-forming substance;- between 0 and 50% of at least one water insoluble particulate mineral material;-between 0 and 95% of an organic solvent;-between 0.01 and 10% of at least one additive selected from the group consisting of
emulsifier, thickener, pH regulating agent, antifoam, dispersant,preservative, fungicide and a combination of the above, and-water until 100% by weight of the formulation is completed, this amount being ablebe equal to zero;
5 such that said concentrated formulation is diluted in water before being applied on the substrate.
[12]
12. The method according to any one of claims 7 to 11, wherein the substrate
Absorbents are wood chips, and the method includes the manufacture of these chips. 10
[13]
13. Use of the substrate for insect control described in any one of claims 1 to 6, as animal beds.
[14]
14. Beds for animals formed by the substrate for the control of flies and other insects described in any one of claims 1 to 6.
[15]
15. Use of the substrate for the control of insects described in any one of claims 1 to 6, for the preparation of an agricultural fertilizer.
FIG. 1st FIG. 1b
(12)
(13)
FIG. 2nd
, I I
- 6
-W ....
eleven'
/
(18)
FIG. 2b
7.3- 4.2
and or1 2 Formulation34
FIG. 3
e 1 234 s
Formulation
FIG. 4
and
-
e 1 2 3 4 5 6
Formulation
FIG. 6
"tl ni" tl
neither
t:
or
E 8
5.8
'*
e o 1 2 3 4
Formulation
FIG. 7

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公开号 | 公开日 | 专利标题

Quarles1992|Diatomaceous earth for pest control

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同族专利:

公开号 | 公开日

CL2019001050A1|2019-09-13|

RU2019111559A3|2021-01-21|

CN110312420A|2019-10-08|

JP2020502257A|2020-01-23|

CA3041399A1|2018-04-26|

AU2017345306A1|2019-05-23|

CO2019003919A2|2019-06-28|

WO2018073472A1|2018-04-26|

EP3530110A4|2020-10-28|

ES2668976B1|2019-02-27|

RU2019111559A|2020-10-19|

EP3530110A1|2019-08-28|

US20180110218A1|2018-04-26|

AR109835A1|2019-01-30|

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ES201600885A|ES2668976B1|2016-10-21|2016-10-21|Substrate for the control of flies and other insects, their manufacturing process and use of the substrate as an animal house.|ES201600885A| ES2668976B1|2016-10-21|2016-10-21|Substrate for the control of flies and other insects, their manufacturing process and use of the substrate as an animal house.|

US15/342,265| US20180110218A1|2016-10-21|2016-11-03|Substrate for Controlling Flies and Other Insects, Method for Manufacturing Thereof and Use of Said Substrate as Animal Litter|

PCT/ES2017/070684| WO2018073472A1|2016-10-21|2017-10-17|Substrate for controlling flies and other insects, production method thereof and use of the substrate as an animal bed|

CA3041399A| CA3041399A1|2016-10-21|2017-10-17|Substrate for controlling flies and other insects, production method thereof and use of the substrate as an animal bed|

AU2017345306A| AU2017345306A1|2016-10-21|2017-10-17|Substrate for controlling flies and other insects, production method thereof and use of the substrate as an animal bed|

CN201780073666.1A| CN110312420A|2016-10-21|2017-10-17|For controlling the purposes of the matrix, its production method and the matrix of flies and other insects as animal beds|

JP2019542805A| JP2020502257A|2016-10-21|2017-10-17|Substrates for controlling flies and other insects, methods for their production and use of said substrates as animal beds|

EP17862564.6A| EP3530110A4|2016-10-21|2017-10-17|Substrate for controlling flies and other insects, production method thereof and use of the substrate as an animal bed|

RU2019111559A| RU2019111559A3|2016-10-21|2017-10-17|

ARP170102906A| AR109835A1|2016-10-21|2017-10-19|SUBSTRATE FOR THE CONTROL OF FLIES AND OTHER INSECTS, ITS PROCESS OF MANUFACTURE AND USE OF SUBSTRATE AS AN ANIMAL BED|

CONC2019/0003919A| CO2019003919A2|2016-10-21|2019-04-17|Substrate for the control of flies and other insects, their manufacturing process and use of the substrate as an animal bed|

CL2019001050A| CL2019001050A1|2016-10-21|2019-04-17|Substrate for the control of flies and other insects, its manufacturing process and use of the substrate as animal litter|

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