• 专利附录
  • 专利说明
  • 权利要求
  • 类似技术
  • 同族专利
  • 引用文献
  • 法律状态
  • 优先权
    • 专利摘要:
    Device for determining the amount of ethanol in low alcoholic beverages. A device is detailed in this document that allows carrying out a series of measurements from data taken in low alcoholic beverages, in order to determine from said measurements the amount of ethanol present in them. The device described here presents a pair of flow subsystems and electrodes governed by a microcontroller in such a way that, through an injection system, it is possible to pass a sample of the beverage to be quantified by a measuring cell. The object of the invention is based on a series of elements such as valves and sensors implemented together with two flow subsystems that allow carrying out the measurement that gives the possibility of determining the amount of ethanol. (Machine-translation by Google Translate, not legally binding)
    公开号:ES2661092A1
    申请号:ES201631254
    申请日:2016-09-27
    公开日:2018-03-27
    发明作者:Ángel Julio REVIEJO；José Manuel PINGARRÓN；Asunción RUIZ BARRIO；Felipe COZUELO；Eva VARGAS；Guillermo José GONZÁLEZ DE RIVERA PECES；Fernando LÓPEZ COLINO；Javier GARRIDO SALAS
    申请人:Universidad Complutense de Madrid；Universidad Autonoma de Madrid；
    IPC主号:
    专利说明:

    OBJECT OF THE INVENTION
     The object of the invention belongs to the technical field of analytics.
    More specifically, the present invention relates to an automated analyzer for the determination of ethanol preferably in beer of the so-called "0.0" by a method that is carried out remotely in real time during the brewing process. . Although the present invention is defined in terms related to beer, the invention is extensible to the determination of other alcoholic beverages with low ethanol content. BACKGROUND OF THE INVENTION
    The industrial scale control of the fermentation or product manufacturing processes of the beer industry is based mainly on the monitoring of ethanol. In order to analyze and validate these processes efficiently, it is necessary to know when and where changes occur in the fermentation and processing process to correct them. Therefore, there is a need to develop rapid and efficient analytical instrumentation that allows real-time monitoring of ethanol. In this context, automatic methods for online measurements meet these requirements and are ideal for monitoring these fermentation processes.
    At present, the monitoring of ethanol, mainly in the case of “0.0” type beers in the beer industry, is carried out offline, since for the detection limits and the level of precision required in this case no There are sensors on the market that meet these requirements. This supposes a high consumption of time and, in addition, prevents the resolution of the problems that can arise in real time, during its manufacture.


    Automated analyzers are based on the use of enzymatic amperometric biosensors as a sensor element.
    The methodologies used to determine the alcoholic strength in beverages can be listed as follows:
    one. Alcohol in beer by distillation. Concentration range: 2.2 to 9.0% (v / v). Accuracy: 0.04%
    2. Alcohol in beer by catalytic combustion. Concentration range 0.188 to 7.25% (v / v) .Precision 0.026%
    3. Alcohol in beer by refractometry. Concentration range 0.82 to 7.37% (v / v). 0.026% accuracy
    Four. Alcohol in beer by gas chromatography. Concentration range 0.84 to 7.24% (v / v). 0.028%
    There is a wide variety of equipment on the market that uses these methodologies for the monitoring of ethanol in beers in particular and in alcoholic beverages in general.
    There is only one company that sells equipment for “on-line” monitoring of alcoholic strength in “0.0” beers. This company is Anton-Paar, (http://www.antonpaar.com/in-en/products/details/online-measurement-of-alcohol-extract-and-originalextract-beer-monitor/) whose precision (0, 02%) is not suitable for the measurement of ethanol content in “0.0” beers.
    The other methodologies and, therefore, the equipment based on them, are not applicable to the determination of the online ethanol content in the brewing process of this type of beer. DESCRIPTION OF THE INVENTION
    To solve the above-mentioned problem, there is a device that can have a biosensor, sensor, analyzer, in short, a device for determining the amount of ethanol in low-grade alcoholic beverages that


    It allows the monitoring of the alcoholic strength in alcoholic beverages, such as beer, a device that can have at least two different configurations. This type of solution also allows real-time measurements to be obtained that allow decisions to be taken in real time to solve problems in said manufacturing process.
    On the one hand, graphite-Teflon composite sensors can be used, and on the other hand, sensors can be used that involve the use of modified electrodes with gold films deposited by means of sputtering techniques.
    The analyzer device of the invention directly drinks the beverage by means of a probe and from there proceeds to measure the content of ethanol in a unit of measurement, providing results in units of volume percent. Also, alarm means are available that indicate when the ethanol content is above a certain limit value; that in the case of non-alcoholic beers, it can be set at 0.04%, which is the quality parameter for this product.
    The device of the invention can have at least two possible configurations depending on the measurement form, a continuous mode analyzer (continuous measurement of the ethanol content) and in discontinuous mode (measurement of the ethanol content at constant time intervals and equal volumes) . In both cases the components and devices will be similar and only the analyzer control method will vary. DESCRIPTION OF THE DRAWINGS
    To complement the description that is being made and in order to help a better understanding of the characteristics of the invention, according to a preferred example of practical implementation thereof, a set of drawings is attached as an integral part of said description. where, for illustrative and non-limiting purposes, the following has been represented:
    Figure 1.- Shows a view of a representative diagram of a preferred embodiment of the object of the invention.


    Figure 2.- Shows a view of a representative diagram of an alternative embodiment of the object of the invention.
    Figure 3.- Shows a perspective view of the measuring cell of the device of the invention.
    Figure 4.- Shows side and plan views of the sample unit of the device of the invention.
    Figure 5.- Shows a view of the degasser of a possible alternative embodiment of the device of the invention.
    Figure 6.- Shows a perspective view of a possible configuration of the object of the invention.
    Figure 7.- Shows a view of what is shown on a screen in response to an analysis carried out using the invention.
    Figure 8.- Shows a screenshot of the results obtained for the continuous mode.
    Figure 9.- Shows a screenshot of the results obtained for the discontinuous mode. PREFERRED EMBODIMENT OF THE INVENTION
    In a preferred embodiment of the object of the invention, and by way of non-limiting example, it is directed to beer of the non-alcoholic type or also called "0.0".
    There are two possible configurations of the analyzer or device for determining the amount of ethanol in low-alcoholic alcoholic beverages of the invention, which comprise a flow system which in turn comprises two flow subsystems. A first flow subsystem is responsible for taking the ethanol separated from the sample to an amperometric detector (17) and a second flow subsystem is the


    responsible for carrying the sample to a measuring cell (1) of the device where the ethanol is separated from the sample and subsequently to waste.
    Both possible configurations of the analyzer, which is automated, comprise a mechanical or hydrodynamic block, an electronic and control block. Next, for the different possible configurations of the analyzer device, its components and each of the blocks will be described.
    The mechanical or hydrodynamic block includes all the components and devices necessary to carry the sample under the appropriate conditions from where the beer “0.0” is being made to an amperometric detector (17) which is located in a cell of flow. The general scheme of the analyzer in continuous mode is shown in Figure 1 and in discontinuous mode in Figure 2.
    In this way it is necessary that in a preferred embodiment of the device of the
    This invention comprises controlled by a microprocessor (19):
    - A first flow subsystem responsible for carrying ethanol separated from the
    shows up to a measuring cell (1) comprising a series of electrodes
    (5,6,7).
    -  A second flow subsystem responsible for taking the sample to a sampling unit (3) where the ethanol is separated from the sample and from there it passes to the measuring cell (1), passing the rest of the sample once the sample is separated. ethanol without passing through the measuring cell (1); where the measuring cell (1) in turn comprises: An input of the sample solution, an output of carrier solution and / or sample, and a reference electrode (5), an auxiliary electrode (6) and an indicator electrode ( 7) connected to an amperometric detector (17) and intended to perform amperometric measurements.
    -  An injection system which in turn comprises: a loop (2) of constant volume, a first solenoid valve (V1) with two inlets being one of them connected to the carrier and an outlet connected to the loop (2), and intended to allow the sample dissolution step to fill the loop (2); said first solenoid valve (V1) presenting two modes of operation: a first mode of operation, called FILLING, where the exit path of


    The first solenoid valve (V1) is in communication with the loop (2) by connecting said exit path of the first solenoid valve (V1) with one of the other two inlets of the first solenoid valve (V1) connected to the input of said loop (2), and a second mode of operation, called INSERTION, in which the other two paths of the first solenoid valve (V1) other than the inlet path are in communication; a second three-way solenoid valve (V2), with an inlet path connected to the loop (2) and two outlets, and intended to allow the solution to be discarded when the first solenoid valve (V1) is in the first mode of operation through an exit path, or to the second flow subsystem when the first solenoid valve (V1) is in the second mode of operation through another exit path; a third three-way solenoid valve (V3), with an inlet path connected to one of the first solenoid valve inlets (V1), another inlet path connected to an outlet path of the second solenoid valve (V2) and intended to allow the passage of a carrier solution, which is a solution not coming from the loop (2), when the first solenoid valve (V1) is in the first mode of operation or allow the passage of the sample solution from the loop (2) to the second flow subsystem when the first solenoid valve (V1) is in the second mode of operation, and - a fourth solenoid valve (V4) with two inlet and one outlet, corresponding a first inlet path to the sample, a second way of entry to the pattern, and the exit path being connected to one of the paths of the first solenoid valve (V1), preferably the entry path, such that, since the first solenoid valve (V1) has one of its paths connectedto the loop (2), the microprocessor (19) manages the alternative communication between one of the inputs and the output in such a way that the passage to the loop (2) of the sample or the pattern is controlled. The injection system also has a first pump (B1) connected to the loop (2) intended to propel the carrier solution through the first flow subsystem to the measuring cell (1), a second pump (B2) connected to a pathway. output of the third solenoid valve (V3) intended to propel solution from the loop (2) to the second flow subsystem when the first solenoid valve (V1) is in the second mode of operation thus allowing the passage of the carrier solution, and a third pump (B3) connected to one of the exit paths of


    the second solenoid valve (V2) other than that exit path connected to the inlet path of the third solenoid valve (V3), such that the filling of the loop (2) with sample solution is allowed and the excess to be disposed of when the First solenoid valve (V1) is in the first mode of operation.
    The device is completed with an amperometric detector (17) connected to the electrodes (5, 6, 7) and adapted to control an applied potential and collect a current value generated in the sensor which allows to obtain the concentration reading of ethanol
    The electrodes used can be: silver / silver chloride electrode as reference electrode (5), stainless steel tube as auxiliary electrode (6) and as an indicator electrode (7) two different designs can be used, graphite enzymatic amperometric- Teflon or stainless steel coated its surface with gold by sputtering, immobilizing enzymes and proper mediator on the electrode surface by entrapment with a dialysis membrane. It is used in both modes of the analyzer. The base of the indicator electrode (7) is located perpendicularly to a flow inlet and said indicator electrode (7) comprises an input (8) of carrier solution, standard or sample and an outlet (9) that goes to the waste. As can be seen in Figure 3.
    An injection system allows constant sample volumes to be inserted. Therefore, it will only be used in the batch analyzer, figure 2. This injection system has the constant volume loop (2), the peristaltic pumps (B1, B2, B3), preferably three and the solenoid valves (V1, V2, V3, V4), preferably four, of three ways each. The first solenoid valve (V1) is used to allow the sample / pattern to pass (according to the flow established by a fourth solenoid valve (V4) to fill the loop (2) in a first mode of operation (FILL position) or carrier solution to insert the sample into the analyzer flow system in a second mode of operation (INSERT position) The second solenoid valve (V2) allows the solution selected by the fourth solenoid valve (V4) to go to waste when the first solenoid valve (V1) ) is in the first mode of operation called FILLING, or go to the flow system in the second mode of operation


    called INSERTION. The third solenoid valve (V3) allows the passage of dissolution not coming from the loop (2), which we will call carrier dissolution, when the first solenoid valve (V1) is in FILLED mode and allows the sample to pass from the loop (2) to the system of flow when the first solenoid valve (V1) is in the second mode of operation called INSERTION. The first pump (B1) is used to propel the carrier solution through the first flow subsystem. The second pump (B2) is used to propel the solution from the loop (2) to the flow system of the analyzer and is constantly running because when it does not propel the solution from the loop (2), the first solenoid valve (V1) in so-called mode INSERTION, allows the passage of the carrier solution to the second flow system. The third pump (B3), works only when the injection system is in the FILLED mode and allows the loop to be filled with the sample / pattern (according to the selection in the fourth solenoid valve (V4)) and the excess is sent to the waste.
    According to this configuration, the carrier, standard or sample solution from the second pump (B2) is introduced alternately through the input (8) according to the configuration of the solenoid valves (V1, V2, V3, V4) and / or two additional solenoid valves (V5, V6). The first additional solenoid valve (V5) has two input channels, one of them connected to the pattern and another to the carrier, and an output path connected to an input path of the second additional solenoid valve (V6), which has another input connected to the sample and an output path connected to the second pump (B2) in such a way that the sample, the carrier pattern or solution is allowed to be introduced into the second flow subsystem.
    A sampling unit (3) as shown in Figure 4 is dedicated to allowing the separation of ethanol from the sample, in addition to producing a dilution effect of ethanol. This sampling unit (3) consists of an ethanol permeable membrane that separates the analyte from the sample, that is, the ethanol passes from the second flow subsystem to the first flow subsystem. This membrane is located between two components that have the same dimensions where it enters; solution inlet (10) the solution, in one of them the sample, second flow subsystem, and in the other the carrier solution, first flow subsystem, which collects the ethanol that crosses the membrane and takes it to the flow cell which is an exit (11). In each of the


    Components have an O-ring (12) to make the area where the membrane is airtight being the two components are equal and symmetrical.
    When you have that the low-alcoholic alcoholic beverage contains gas, you can use a degasser (4), such as the one shown in the scheme in Figure 5, which allows you to eliminate bubbles from beer; to carry out this task, the degasser (4) is provided with a degasser tank (14), an inlet of the degasser (13) and two outlets of the degasser (15,16). The inlet of the degasser (13) serves so that the ungassed beer reaches the degasser tank (14) where the bubbles are formed, the formed gas exits through a first outlet of the degasser (15) while through a second outlet of the degasser
    (16) the degassed beer is taken to the second flow subsystem. The beer reaches the degasser through the pressure exerted by the beer in the sampling area.
    In that embodiment in which the device of the invention operates in continuous mode, use is made of the two additional solenoid valves (V5, V6) that allow the sample, the ethanol standard or the carrier to be introduced into the second flow subsystem.
    In order to be able to control the aforementioned elements, the electronic and control block is used, which comprises a control electronics that allows controlling all the instrumentation variables of the automated analyzer, as well as the different electromechanical components mentioned above. To carry out this function, a configuration such as the one shown in Figure 6 is used, where a microprocessor (19) is observed to carry out the acquisition and treatment of the data transmitted by the analyzer, as well as the control of the amperometric detector (17) and the active management interface, and an instrumentation management interface (18) that allows the control of the active electromechanical components of the analyzer flow system (solenoid valves (V1, V2, V3, etc.). V4) and pumps (B1, B2, B3).
    The described electronics is connected to the amperometric detector (17), which is a potentiostatic system that is in turn connected to the electrodes (5, 6, 7) of the measuring cell (1) and that controls the applied potential and collects the intensity of


    current generated in the sensor which will determine the final ethanol concentration value.
    In order to interact with the operator and visualize in real time the information obtained during the monitoring process of the ethanol content in beer “0,0”, a screen is used as shown in Figure 7.
    In that embodiment of the invention in which an analysis is carried out in continuous mode having device of the invention configured in continuous mode; once the automated sensor is put into operation, the carrier solution is passed through the two flow subsystems in order to obtain a baseline. Once a stable baseline is reached, a carrier solution, standard or sample of known ethanol concentration is introduced into the second flow subsystem, which in this case is 0.04% (allowable limit for this type of beer) with in order to calibrate the equipment. To obtain the analytical signal, the ethanol of the second flow subsystem crosses the membrane of the sampling unit and passes to the first flow subsystem that is carried by the carrier solution to the biosensor to reach the steady state which is the analytically useful signal. Once the steady state for the standard solution (known ethanol concentration) is reached, the analyzer reintroduces the carrier solution into the second flow subsystem until it reaches the stationary baseline again. Subsequently, from the probe placed at the sampling point of beer "0.0" the sample is passed to the second flow subsystem continuously, the ethanol crosses the membrane and is collected by the carrier solution that takes it to the cell of flow where the continuous ethanol signal is obtained for the sample solution, until it reaches the steady state.
    The signal is then continuously recorded until one of these two conditions occurs, (i) that 1 hour of beer has passed through the flow cell and (ii) that there is a variation of more than 10% in the intensity of current measured both below and above 0.04% of the ethanol content, at which time the analyzer is recalibrated, device for determining the amount of ethanol in low-grade alcoholic beverages of the invention, passing standard solution of 0.04% ethanol. In all cases it is recorded graphically and the


    Attachment with the data shown in Figure 8.
    In this way, values of the coefficient of variation of around 5.0% and confidence intervals of about ± 0.001% (v / v) are obtained. The values obtained for the monitoring of a series of beers "0,0" with the device of the invention have been compared with the results obtained by gas chromatography, which is the official methodology for this type of analysis. The results obtained are shown in the following table:
    Sample [EtOH],% gas chromatography[EtOH],% BioanalyzerDifferenceۄ expt ۄttab
    Beer “0,0” - 1 Beer “0,0” - 2 0.006 ± 0.002 0.0034 ± 0.00070.005 ± 0.001 0.0038 ± 0.00090.001 -0,0004
    Beer “0,0” - 3 0.025 ± 0.0050.021 ± 0.0040.0040.6642,132
    Beer "0,0" doped 0,04
    0.043 ± 0.007 0.045 ± 0.002 -0.02
    %
    Beer “0,0” doped 0,08
    0.086 ± 0.005 0.085 ± 0.006 0.001
    %
    Confidence intervals have been calculated for a significance level of 0.05.
    To carry out this analysis, different instrumental parameters are provided
    15 to operate the analyzer through the screen that is interactive and allows data entry. In addition to processing the data acquired by the microprocessor (19) and proceed to activate the alarms when necessary.
    Considering first the user interface elements, which should
    20 provide information to the operator during the monitoring of ethanol in beer, the operator has access to the following information:


    - Registration parameters (20): This block shows the experimental variables that are being applied, such as the working potential, the time interval for capturing amperometric current data and the duration of the analysis.
    -  Process indicator (21): This block shows a simplified flow system diagram that allows you to keep track of what solution is coming to the sensor at all times or what type of measurement is to be performed.
    -  Graphic block (22): Section of the screen where the intensity-time record that is obtained is represented in real time.
    -  Ethanol concentration in beer (23): Window showing the concentration measures determined in the sample as they are being recorded.
    -  Standard concentration (24): Window where the concentration of the standard solution that is used as a reference for the calculation of the ethanol concentration in the sample is configured.
    Next, the operator inserts into the device of the invention a series of configuration parameters from which it is possible to carry out a method of analysis and operation thereof and, thus, analysis and decision making. The parameters to be configured are the following:
    x T_S_AUTO_CALIB. Time in seconds after which the system will automatically recalibrate. It is set only in continuous mode.
    x T_S_REPOSO. Time in seconds used to measure the current intensity corresponding to the carrier solution to verify the stability of the baseline. It is configured in both continuous and discontinuous mode.
    x P_DESVIACION. Percentage of deviation of the maximum allowed sample reading between two consecutive measurements before forcing a recalibration. It is configured in both continuous and discontinuous mode.
    x P_ LEVEL. Maximum slope of the curve in absolute value that is tolerated to consider that the current intensity corresponding to the baseline is stable. It is configured in both continuous and discontinuous mode.
    x T_S_REINICIO. Time applied between analyzer restarts. It is configured in both continuous and discontinuous mode.
    x T_N_ESPERA. Time taken for baseline stabilization to


    that the system begins to verify the stabilization of the current intensity, before the introduction of standard solution or sample. It is configured in both continuous and discontinuous mode.
    x T_N_ESPERA_MUESTRA. Time taken since the system has made a change in the valves for the introduction of standard or sample until it begins to verify the stabilization of the recorded current intensity (and in the case of the sample, the reading of the concentration of ethanol). It is set only in continuous mode.
    x N_MEDIDAS. Number of sample measurements to be performed before recalibrating with standard solution. It is set only in batch mode.
    x T_S_BETA. Time in seconds during which the extreme value of the amperometric peak is sought. It is set only in batch mode.
    x T_S_CLEAN / T_M_FASE. Continuous / Repetitive Time in minutes used to clean the different inputs of the flow system in each phase of the cleaning program. It is configured in both continuous and discontinuous mode.
    x N_N_MEDIDA. Number of measurements to be taken of the sample in each iteration. It is set only in batch mode.
    x P_ALCOHOL_MAX. Maximum percentage of ethanol allowed in the measure of concentration of a sample. It is configured in both continuous and discontinuous mode.
    x T_S_CEBADO / T_S_CEBADO SAMPLE. Time in seconds used to fill the pattern or sample loop. It is set only in batch mode.
    x D_VARIANZA. Percentage of deviation from the maximum current intensity value allowed during baseline stabilization without considering that the sensor should be changed. It is configured in both continuous and discontinuous mode.
    x T_S_MARGEN_ALCOHOL. Time in seconds during which it is evaluated whether the level of ethanol has exceeded the allowed value. After this time, if it exceeds the established ethanol concentration value, a recalibration is forced. It is set only in continuous mode.
    x P_TOLERANCIA_PATRON. Percentage of maximum signal deviation allowed between two consecutive measurements of the pattern before forcing a


    recalibrated It is configured in both continuous and discontinuous mode.
    x T_S_CAMBIO_PENDIENTE. Time in seconds used to verify the stability of the baseline (corresponding to the carrier solution). In this period, measure the current slope of the curve, and
    5 considers that the change in slope is sufficient to start reading the measurement. It is configured in both continuous and discontinuous mode.
    x N_CHANGE_PENDING. Factor applied in the calculation of the slope of the amperometric curve to consider that the measurement reading can begin. It is configured in both continuous and discontinuous mode.
    10 The results obtained in addition to providing the result of the concentration of ethanol in% (v / v), an attachment is created where the following parameters are shown. Depending on the mode used, the results obtained in the file are for the continuous mode (Figure 8): date and time of registration
    15 (25) and the concentration in% (v / v) (26). And for the discontinuous mode shown in Figure 9: date and time of recording (27), peak current intensity (28), concentration in% (v / v) (29) and type of measurement (30).

    权利要求:
    Claims (5)
    [1]
     R E I V IN D I C A C I O N E S
    1. Device for determining the amount of ethanol in low-alcoholic alcoholic beverages, the device being characterized in that it comprises controlled by a microprocessor (19):
    - a first flow subsystem responsible for carrying the ethanol separated from the sample to a measuring cell (1) comprising a series of electrodes (5,6,7),
    -  a second flow subsystem responsible for taking the sample to a sampling unit (3) where the ethanol is separated from the sample and from there it passes to the measuring cell (1), passing the rest of the sample once the sample is separated ethanol without passing through the measuring cell (1); where the measuring cell (1) in turn comprises:
    -  a solution inlet shows,
    -  a carrier solution solution and / or sample, and
    -  a reference electrode (5), an auxiliary electrode (6) and an indicator electrode (7) connected to an amperometric detector (17) and intended to perform amperometric measurements,
    -  an injection system that in turn comprises:
    -  a constant volume loop (2),
    -  a first solenoid valve (V1) with two inlets being one of them connected to the carrier and an outlet connected to the loop (2), and intended to allow the sample dissolution step to fill the loop (2); said first solenoid valve (V1) presenting two modes of operation: a first mode of operation, called FILLING, where the exit path of the first solenoid valve (V1) is in communication with the loop (2) connecting said exit path of the first solenoid valve (V1) with one of the other two input channels of the first solenoid valve (V1) connected to the input of said loop (2), and a second mode of operation, called INSERTION, in which the two other paths of the first solenoid valve (V1) other than the inlet path,
    -  a second three-way solenoid valve (V2), with an inlet path
    connected to the loop (2) and two output paths, and intended to allow 16

    passage of the solution to waste when the first solenoid valve (V1) is in the first mode of operation through an outlet path, or to the second flow subsystem when the first solenoid valve (V1) is in the second mode of operation through from another exit route,
    -  a third three-way solenoid valve (V3), with an inlet path connected to one of the first solenoid valve inlets (V1), another inlet path connected to an outlet path of the second solenoid valve (V2) and intended to allow the passage of a carrier solution, which is a solution not coming from the loop (2), when the first solenoid valve (V1) is in the first mode of operation or allow the passage of the sample solution from the loop (2) to the second flow subsystem when the first solenoid valve (V1) is in the second mode of operation,
    -  a fourth solenoid valve (V4) with two inlets and one outlets, a first inlet path corresponding to the sample, a second inlet path to the pattern, and the outbound path being connected to one of the tracks in the first solenoid valve (V1), preferably the inlet path, such that, since the first solenoid valve (V1) has one of its tracks connected to the loop (2), the microprocessor
    (19) manages the alternative communication between one of the inputs and the output in such a way that the passage to the loop (2) of the sample or the pattern is controlled,
    -  a first pump (B1) connected to the loop (2) intended to propel the carrier solution through the first flow subsystem to the measuring cell (1),
    -  a second pump (B2) connected to an outlet path of the third solenoid valve (V3) intended to propel solution from the loop (2) to the second flow subsystem when the first solenoid valve (V1) is in the second mode of operation allowing thus the passage of the carrier solution, and
    -  a third pump (B3) connected to one of the output paths of the second solenoid valve (V2) other than that output path connected to the input path of the third solenoid valve (V3), such that the filling of the loop (2) with sample solution and carry the

    excess to waste when the first solenoid valve (V1) is in the first mode of operation, and
    -  an amperometric detector (17) connected to the electrodes (5,6,7) and adapted to control an applied potential and collect a current intensity value generated in the sensor which allows to obtain the ethanol concentration reading.
    [2]
    2. Device for determining the amount of ethanol in low-alcoholic alcoholic beverages according to claim 1 characterized in that:
    - the reference electrode (5) is a silver / silver chloride electrode,
    - the auxiliary electrode (6) is a stainless steel tube like, and
    - The indicator electrode (7) is of the graphite-Teflon enzymatic amperometric type
    or stainless steel coated on its surface with gold.
    [3]
    3. Device for determining the amount of ethanol in low-alcoholic alcoholic beverages according to claim 1 or 2, characterized in that: x the measuring cell (1) comprises an inlet (8) of carrier solution, standard or sample and an outlet (9) that goes to waste, and
    x the indicator electrode (7) is located perpendicular to the flow inlet.
    [4]
    Four. Device for determining the amount of ethanol in low-alcoholic alcoholic beverages according to any one of the preceding claims, characterized in that it additionally comprises a first additional solenoid valve (V5) with two inlets, one of them connected to the standard and the other to the carrier, and an output path connected to an input path of a second additional solenoid valve (V6) which has another input path connected to the sample and an output path connected to the second pump (B2) in such a way that the sample, the carrier pattern or solution is introduced into the second flow subsystem.
    [5]
    5. Device for determining the amount of ethanol in low-grade alcoholic beverages according to any one of the preceding claims wherein the low-grade alcoholic beverage contains gas, the device characterized in that it additionally comprises a degasser (4) intended to eliminate the

    bubbles of the alcoholic beverage of low graduation and which in turn includes:
    - an inlet of the degasser (13) so that low-grade alcoholic beverage without degassing reaches a tank of the degasser (14) where bubbles are formed,
    5 -a first outlet of the degasser (15) intended to allow the exit of gas from the degasser tank (14), and
    - a second outlet of the degasser (16) intended to bring the degassed low alcoholic beverage to the second flow subsystem by connecting said second outlet of the degasser (16)
    10 to the first solenoid valve (V1) or to the fourth solenoid valve (V4).

     DRAWINGS 





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