• 专利附录
  • 专利说明
  • 权利要求
  • 类似技术
  • 同族专利
  • 引用文献
  • 法律状态
  • 优先权
    • 专利摘要:
    System and method for muscular, tendinous, cartilaginous and bone regeneration with autologous micro-grafts of stem cells, progenitors and cells of the stromal vascular system and their use. The system and the method for such regeneration of these tissues, is based on the mechanical disintegration of autologous tissues such as the dermis, muscle, perichondrium and cartilage of stem cells and other cellular components of the stromal vascular system (sve), obtained through mechanical processing. Of the sample, and developing first by disintegrating the tissue and filtering the stem cells, followed by disinfection of the area to be treated and application of the solution obtained as close to the injured area or intra-articular injection if the lesion is located within of the joint region. It includes the use of the micro-grafts thus obtained for trauma regenerative, rehabilitative or sports medicine therapy. (Machine-translation by Google Translate, not legally binding)
    公开号:ES2652135A1
    申请号:ES201600624
    申请日:2016-07-29
    公开日:2018-01-31
    发明作者:José Miguel CASANOVA ROSELL
    申请人:José Miguel CASANOVA ROSELL；
    IPC主号:
    专利说明:

    SYSTEM AND METHOD FOR MUSCLE REGENERATION, TENDINOSA,CARTILAGINOUS AND 6SEA WITH AUT6LOGUE MICROINJUTS OFMOTHER CELLS AND ITS USE
    OBJECT OF THE INVENTION
    The present invention relates to a system and method for muscle, tendon, cartilaginous and bone regeneration with micro grafts autologous stem cells, progenitors, stromal vascular system cells, the system comprising a machine that operates at a constant speed of approximately 80 rpm, which operates in conjunction with a kit, which is a disposable medical device with CE certification, containing a grid of stationary stainless steel with approximately 100 holes Hexagonal and around each hole 6 micro blades perform the cutting and filtering of hard and soft tissues. It is also the object of the invention to use said stem cells as autologous micro grafts for the regeneration of the tissues described.
    In all tissues, such as the skin, or the hair follicle, muscle, perichondrium of the facial cartilage, fat, etc. There are a large number of stem cells, which have a restorative-regenerative function and that is why it makes sense to apply them to tissues that require regeneration, due to some type of lesion.
    The advantages of the present invention over the previously known are the following:
    The method is very effective against clinical signs such as pain, stiffness and inflammation, stopping it and causing the treated tissues to revitalize and regenerate in the initial, non-scar stage.
    With just one session it is sufficient if the diagnosis and technique are correctly performed, repeat in case of reproduction of clinical signs of the pathology. The method is performed quickly, in just 30 minutes the patient can return to their chores It can be done in a medical consultation without the need for an operating room or a sterile room, although in a sterile field. The tissue manipulation is brief and simple, it does not exceed 90 seconds. The method has no side effects or sequelae. It is much cheaper than any other known method.
    The industrial application of the present invention is in the field of traumatic regenerative medicine, rehabilitation and sports medicine and more specifically in regenerative medicine by autologous micro grafts of stem cells.
    BACKGROUND OF THE INVENTION
    Although no invention identical to that described has been found, we present below found documents that reflect the state of the art related to it.
    Thus, document ES234 7078T3 refers to an implantable material, comprising a biocompatible matrix and endothelial cells anchored or included, endothelial type cells or their analogs to be used in the reduction of an immune response or an inflammatory reaction in a method comprising the step of providing said implantable material to a receptor in an amount sufficient to reduce the immune response or inflammatory reaction in said receptor, wherein the matrix has a three-dimensional configuration such that anchored and / or included cells can create and occupy a multidimensional habitat In this case, the implantable material comes from a biocompatible matrix, unlike in the proposed invention, which is autologous micro grafts of progenitor and regenerating cells.
    ES2364957T3 proposes the use of a concentrated population of regenerative cells obtained from adipose tissue comprising stem cells in the preparation of a medicament to promote epithelialization and the formation of neo dermis at a wound site, in which said concentrated population of cells is It must be administered to a patient who needs it without culturing the cells before the cells are administered. In comparison with the invention proposed in this specification, the comparison refers to a use of regenerative cells obtained from adipose tissue comprising stem cells, for the formation of neo dermis at a wound site, while the invention proposed here refers to a use of stem cells of the same embryonic strain, ectoderm, so there are more unipotential and multipotential cells that do not need to differentiate, being the tissue solution processed and ready to inject in just 20 minutes, with minimal manipulation, not requiring an operating room, no sequelae remaining and being the most economical and fastest procedure.
    ES2545385T3 refers to a stem cell processing unit derived from autonomous adipose tissue, comprising: a tissue removal vessel that is configured to receive unprocessed adipose tissue that is removed from a patient, wherein said vessel for extracting tissue. tissue is defined by a closed system; a first filter that is placed inside said tissue removal container, wherein said first filter has a pore size of about 20 µm to about 5 mm and wherein said first filter is configured to retain a first component of said unprocessed adipose tissue and passing a second component of said unprocessed adipose tissue, such that said first filter separates said first component from said second component, and wherein said first component comprises a population of cells comprising tissue derived stem cells adipose and said second component comprises lipid, blood, mature, and saline adipocytes; a cell extraction vessel, which is configured to receive said first component comprising a population of cells comprising stem cells derived from adipose tissue from said tissue extraction vessel, wherein said cell extraction vessel is within said system closed; a conduit configured to allow the passage of said first component comprising a population of cells comprising stem cells derived from adipose tissue from said container for tissue extraction to said container for cell extraction while maintaining a closed system; a cellular concentrator placed within said cell extraction vessel, which is configured to facilitate the concentration of said first component comprising a population of cells comprising stem cells derived from adipose tissue to obtain a concentrated population of cells comprising stem cells derived of adipose tissue, wherein said cellular concentrator comprises a centrifuge or a rotating membrane filter; and an outlet configured to allow aseptic removal of said concentrated population of cells comprising stem cells derived from adipose tissue. In this case the procedure proposed by the present invention is much faster, it uses stem cells of the same embryo excision, ectoderm, so there are more unipotential and multipotential cells that do not need to differentiate, being ready in just 20 minutes and the tissue solution ready to inject, with minimal manipulation, no operating room, no sequelae and much cheaper.
    Conclusions: As can be seen from the research carried out, none of the documents found solves the problems raised as the proposed invention does.
    DESCRIPTION OF THE INVENTION
    The system for the regeneration of muscular, tendon, cartilaginous and bone tissue based on autologous micro grafts of stem cells from the safe and standardized preparation of samples for the automation and mechanical disintegration of solid human tissues, is constituted from a machine and a kit, the machine being a compact unit that works together with the kit and operates at a constant speed of approximately 80 rpm, while the kit is a disposable medical device containing a stationary stainless steel rack with approximately 100 hexagonal holes and around each hole 6 micro blades for cutting hard and soft tissues to the passage of a stainless steel propeller that rotates and causes the tissue to disintegrate over said holes and seep together with the solution added to a lower receptacle, from which the cellular content is extracted by absorption with a syringe.
    The method for the regeneration of muscle, tendon, cartilage and bone based on autologous micro grafts of stem cells is based on the mechanical disintegration of autologous tissues containing stem cells and other cellular components of the stromal vascular system (EVS), obtained by processing Autologous micro graft mechanic with the patented device as "biological material disintegrating device for the preparation of cell suspensions and tissue micro grafting" with number WO 2016/097960 A2
    To carry out this method, it is essential that the patient is correctly diagnosed with acute, non-scarring tendon, muscle, cartilage or bone lesions. The muscular and tendon lesion should not exceed six months of evolution and as for cartilage, it should not exceed stage 2-3 of lacia articular chondroma and stage 1-2 of osteoarthritis.
    a) In a first stage, a sample of donor tissue, muscle, fat, dermis, facial cartilage is treated, preferably extracted from the posterior region of the ear shell in the case of cartilage regeneration treatment, separating then the dermis of the epidermis manually (de-deridermization) with scalpel or scissors and cutting the autograft for the best mechanical processing of the sample.
    b) Said tissue, is treated under maximum conditions of sterility and is processed in the machine of the system described above, previously adding physiological serum, 1.2 cc maximum or any other solution that acts as a vehicle to be processed and infiltrated later and not damage the viability of the cell content of the suspension and thus allow the disintegration of the tissue and the filtering of the stem cells.
    c) Once the preparation is obtained, the solution obtained by the system described above can be used, in a period not exceeding half an hour if possible, so as not to decrease the viability of the cellular content, by intra-articular injection into the case of chondromalacia or osteorarthrosis or by subcutaneous injection, as close as possible to the tendon or muscle lesion.
    The object of the present invention is also the use of autologous micro grafts of stem cells and stromal vascular system cells obtained by the system and by the method described above for the regeneration of muscle, tendon, cartilaginous and bone tissues.
    BRIEF DESCRIPTION OF THE DRAWINGS
    5 For a better understanding of the description of the procedure,accompany some drawings that represent a preferred embodiment of thepresent invention These drawings show representations.schematics of:
    Figure 1: Obtaining a small tissue sample (1-2 mm 10 to 10 mm)
    Figure 2: Wetting with sterile physiological solution
    Figure 3: Placing the tissue with 1 ml of sterile physiological solution on the rack
    Figure 4: Capping and activating the rotation of the filter to separate the tissue
    Figure 5: Collection of the micro graft suspension with a syringe from the hole provided for this purpose.
    Figure 6: Immediate application. Depending on the end of the treatment, it can be applied only, together with PRP or after mixing with biomaterial.
    The numerical references correspond to the following elements of the invention:
    one. Tissue sample
    2. Sterile physiological solution
    3. Grating
    Four. Filter
    5. Syringe
    6. Application
    DESCRIPTION OF A PREFERRED EMBODIMENT
    A preferred embodiment of the present invention can be based on a system for muscle, tendon, cartilaginous or bone regeneration based on autologous stem cell micro grafts from the safe and standardized preparation of samples (1) for automation and disintegration Mechanics of solid human tissues, is constituted from a machine and a kit, the machine being a compact unit that works together with the kit, operating at a constant speed of approximately 80 rpm, while the kit is a disposable medical device that It contains a grid (3) of stationary stainless steel with approximately 100 hexagonal holes and around each hole 6 micro blades for cutting hard and soft tissues to the passage of a stainless steel propeller that is the one that rotates and makes the fabric disintegrate over said holes and filter together with the solution added to a lower receptacle, from which the container is removed cell nest by absorption with a syringe.
    The method for muscle, tendon, cartilaginous or bone regeneration based on autologous stem cell micro grafts is based on the mechanical breakdown of autologous tissues such as the dermis, muscle, perichondrium and cartilage of stem cells and other cellular components of the stromal vascular system (EVS), obtained by means of the mechanical processing of autologous micro grafts with the patented device as a "device for separating biological material for the preparation of cell suspensions and tissue micro grafting" under number WO 2016/097960 A2.
    To carry out this method, it is essential that the patient is correctly diagnosed with acute tendon, muscle or osteomalacia grade 1 and 2 lesions or osteoarthrosis in the initial stages.
    a) In a first stage, a sample of donor tissue, muscle, fat, dermis, facial cartilage is treated, preferably extracted from the posterior region of the ear shell in the case of cartilage regeneration treatment, separating then the dermis of the epidermis manually (de-deridermization) with scalpel or scissors and cutting the autograft for the best mechanical processing of the sample.
    b) Said tissue, is treated under maximum conditions of sterility and is processed in the machine of the system described above, previously adding physiological serum, 1.2 cc maximum or any other solution that acts as a vehicle to be processed and infiltrated later and not damage the viability of the cell content of the suspension and thus allow the disintegration of the tissue and the filtering of the stem cells.
    c) Once the preparation is obtained, the solution obtained by the system described above can be used, in a period not exceeding half an hour if possible, so as not to decrease the viability of the cellular content, by intra-articular injection into the case of chondromalacia or osteorarthrosis or by subcutaneous injection, as close as possible to the tendon or muscle lesion.
    The object of the present invention is also the use of autologous micrografts of stem cells and stromal vascular system cells obtained by system and by the method described above for the so-called medically, traumatic, rehabilitative or sports medicine regenerative therapy.
    权利要求:
    Claims (4)
    [1]

    [1]
    1.-System for muscle, tendon, cartilaginous or bone regeneration with autologous micro grafts of stem cells, from samples (1) for the automation and mechanical disintegration of solid human tissues, characterized by being a machine and a kit , the machine being a compact unit that works together with the kit, operating at a constant speed of approximately 80 rpm, while the kit is a disposable medical device containing a stationary stainless steel rack (3) with approximately 100 hexagonal holes and around each hole 6 micro blades for cutting hard and soft tissues to the passage of a stainless steel propeller.
    [2]
    2.-Method for muscle, tendon, cartilaginous and bone regeneration with autologous micro grafts of stem cells, based on the mechanical disintegration of autologous tissues such as the dermis, muscle, perichondrium and cartilage of stem cells and other cellular components of the stromal vascular system (EVS), obtained by means of the mechanical processing of autologous micro grafts with the patented device as a "device for separating biological material for the preparation of cell suspensions and tissue micro grafting" number WO 2016/097960 A2, characterized by being developed in the following stages :
    a) In a first stage, a sample of donor tissue, muscle, fat, dermis, facial cartilage is treated, preferably extracted from the posterior region of the ear shell in the case of cartilage regeneration treatment, separating then the dermis of the epidermis manually (de-deridermization) with scalpel or scissors and cutting the autograft for the best mechanical processing of the sample.
    b) In a second stage, said tissue is treated under maximum conditions of sterility and processed in the machine of the system described above, previously adding physiological serum, 1.2 cc maximum or any other solution that acts as a vehicle
    5 to be able to be processed and infiltrated later and not to damage the viability of the cell content of the suspension and thus allow the disintegration of the tissue and filtering of the stem cells.
    c) Once the preparation is obtained, the solution obtained by the system described above can be used in a period
    10 not more than half an hour if possible, so as not to reduce the viability of the cellular content, by intra-articular injection in the case of chondromalacia or osteorarthrosis or by subcutaneous injection, as close as possible to the tendon or muscle lesion.
    [3]
    3.-Use of autologous micro grafts of stem cells obtained by the system and by the method described above for traumatic, rehabilitative or sports medicine regenerative therapy.
    1 ~
     FIG 1
    FIG 2
     FIG 3 
     FIG 4 
     FIG 5 
    FIG 6
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    同族专利:
    公开号 | 公开日
    ES2652135B1|2018-07-30|
    EP3275449A3|2018-02-21|
    EP3275449A2|2018-01-31|
    引用文献:
    公开号 | 申请日 | 公开日 | 申请人 | 专利标题
    ES2121230T3|1993-09-28|1998-11-16|Consul T S S R L|A MECHANICAL CRUSHER FOR BIOLOGICAL MATERIAL.|
    US20060057126A1|2004-09-16|2006-03-16|Nikolai Tankovich|Device and method for hair growth from stem cells|
    WO2014039697A1|2012-09-06|2014-03-13|The Gid Group, Inc.|Tissue processing apparatus and method for processing adipose tissue|
    WO2016097960A2|2014-12-15|2016-06-23|Human Brain Wave S.R.L.|Disgregating device of biological material and corresponding manufacturing method and method for the preparation of cell suspensions and tissue micrografts|
    CN109351422B|2018-11-28|2020-12-11|河南科技大学第一附属医院|Particle medicine grinding equipment for pediatrics|
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    优先权:
    申请号 | 申请日 | 专利标题
    ES201600624A|ES2652135B1|2016-07-29|2016-07-29|System and method for muscle, tendon, cartilaginous and bone regeneration with autologous stem cell micrografts and their use|ES201600624A| ES2652135B1|2016-07-29|2016-07-29|System and method for muscle, tendon, cartilaginous and bone regeneration with autologous stem cell micrografts and their use|
    EP17183807.1A| EP3275449A3|2016-07-29|2017-07-28|System and method for regenerating muscles, tendons, cartilage and bone by means of autologous micrografts of stem cells, progenitor cells, and cells of the vascular stromal system, and use thereof|
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