• 专利附录
  • 专利说明
  • 权利要求
  • 类似技术
  • 同族专利
  • 引用文献
  • 法律状态
  • 优先权
    • 专利摘要:
    Biosensor electrocrºmico. The present invention relates to a biosensor formed by an anode of a conductive material containing at least one enzyme immobilized on its surface and a redox mediator and a cathode of a transparent material containing an electrochromic molecule that changes color when its state changes of oxidation. An ion conducting medium is deposited between the anode and the cathode, which may be in the form of a gel or a permeable membrane. This biosensor is automatically activated when a sample containing the analyte is deposited, whose oxidation/enzymatic reduction generates a flow of electrons from the anode to the cathode that causes the color change of the electrochromic material and which immediately informs the user of the presence of said analyte in the sample. (Machine-translation by Google Translate, not legally binding)
    公开号:ES2623080A1
    申请号:ES201531788
    申请日:2015-12-10
    公开日:2017-07-10
    发明作者:Francisco Javier DEL CAMPO GARCÍA；Antón GUIMERÁ BRUNET；María KITSARA；Miguel ALLER PELLITERO
    申请人:Consejo Superior de Investigaciones Cientificas CSIC；
    IPC主号:
    专利说明:

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    Electrochromic biosensor
    DESCRIPTION
    The present invention relates to a biosensor formed by a first electrode of a conductive material containing at least one enzyme and a redox mediator immobilized on its surface and a second electrode of a transparent material containing an electrochromic molecule that changes color when it changes its oxidation state. Between both electrodes an ion conductive medium is deposited which may be in the form of a gel or permeable membrane. Therefore, the present invention can be encompassed in the field of electrochemistry.
    STATE OF THE TECHNIQUE
    There is a growing need for tools that allow the rapid diagnosis of certain diseases (diabetes and cardiovascular problems mainly). Most of these systems for rapid tests are based on one of two technologies: on the one hand we find colorimetric test strips, and on the other hand we find tests based on a series of reagents and a small measuring instrument or reader. Colorimetric tests are usually economical and very easy to use, but in most cases they only offer qualitative information, and are used primarily to discriminate positive cases from negative cases. On the other hand, the systems based on the combination of reagents and a reader, although they are capable of offering quantitative or at least semi-quantitative responses, have the disadvantage of considering a more complex management by needing a reader that, in addition, can be expensive. The paradigmatic example of these measurement systems, based on test strips plus a reader, is the glucometer.
    A large number of companies and research groups have worked to improve both technologies, although following different directions. In the case of reagent and reader based systems, thanks to the availability of abundant energy sources, such as batteries and batteries, the improvements have sought to simplify use, improve information management, and reduce the volume of sample needed for your analysis. On the other hand, in the case of test-based systems
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    colorimetric, the most sought objective has been the quantification of the sample. To do this, inevitably, all efforts and approaches have been based on conventional detection techniques, mainly optical and electrochemical. The problem with this is that when a measuring device is introduced into the system, the costs per analysis soar and commercial interest is lost. To date, the only product on the market that allows the quantification of a lateral flow test is a system for birth control that identifies the most fertile days of the woman's menstrual cycle. However, this system is complex, expensive and has a significant environmental impact.
    The concept of self-powered sensors arises for the first time in the 1970s in the nuclear industry. However, the first self-powered electrochemical sensor does not arrive until 2001 in the form of a biosensor (J. Am. Chem. Soc., 2001, 123, 10752-10753) where enzymatic fuel cells capable of generating electricity were described from glucose or lactate present in a sample. However, the low amount of energy that these systems were capable of generating did not allow the necessary additional functions to be supplied: mainly a control electronics and some way of making the sensor information available to the user.
    In US2014 / 0322617 and US2015 / 0126834 a biological fuel cell is described which comprises a substrate that adheres to the user's skin, an anode that includes a catalyst that facilitates the conversion of a substrate present in a biological fluid with the consequently liberation of electrons and a cathode formed by an electroconductive material that contains a product capable of reducing an oxygenated substance. The anode and the cathode are separated by an interface that allows the flow of electrons.
    Liu H. et al described a sensor for detecting glucose in artificial urine that uses an electrode with Prussian blue electrodeposited on a thin layer of ITO as an electrochromic indicator and a carbon ink electrode (Anal. Chem. 2012, 84, 2528- 2532).
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    Jia, W. et al described an epidermal device to detect certain substances in the sweat formed by an anode containing the enzyme lactate oxidase, CNTs, TTF protected by a chitosan membrane and a cathode formed by a carbon and platinum material (Angew Chem. Int. Ed. 2013, 52, 7233-7236).
    Zloczeska, A. et al. described a biosensor to detect ascorbic acid based on an anode that is an ITO electrode modified with carbon nanomaterials and a cathode with a silicate matrix where the bilirubin oxidase enzyme is immobilized and protected with a permeable membrane (Biosensors and Bioelectronics , 54 (2014) 455-461).
    More recently, Pinyou et al. Have presented a self-powered device consisting of a glucose fuel cell whose current serves to reduce a transparent electrode modified with methylene green. The color change, which is measured by a spectrophotometer, is proportional to the concentration of glucose present in the sample within a range between 0 and 1mM (Pinyou, P. et al. Coupling of an enzymatic biofuel cell to an electrochemical cell for self-powered glucose sensing with optical readout Bioelectrochemistry 106, 22-27 (2015)).
    DESCRIPTION OF THE INVENTION
    The inventors have developed a biosensor that overcomes the difficulties existing in the field and is composed of a galvanic cell or battery in which one of its electrodes is an electrochemical sensor and the other an electrochromic visor. Thus, given the configuration in the form of a stack (the potential of the cathode is greater than the potential of the anode) by adding a sample containing a substrate for the enzyme present in the system, the sensor is automatically activated, giving direct information to the user at the same time. that an electric power is generated available for other functions.
    Therefore, a first aspect of the invention relates to a biosensor comprising the following elements:
    a) a first electrode formed by a base of a conductive material comprising at least one type of enzyme immobilized on its surface and at the
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    less a redox mediator that facilitates the exchange of charge between the enzyme and the electrode on which it is immobilized;
    b) a second electrode formed by a transparent conductive material on which an electrochromic material has been deposited;
    c) an ion conductive medium, located between both electrodes.
    The first electrode may be made of a conductive material that has good electrocatalytic properties, preferably carbon in any of its forms, such as graphite, carbon nanotubes, or graphene, electrodes based on noble metals such as gold or platinum, or other conductive materials commonly used in the construction of electrochemical devices, such as indium and tin oxide (ITO), or conductive polymers such as PEDOT: PSS, polyanilines, pyrroles, etc.
    In a preferred embodiment, the first electrode further comprises carbon materials such as graphene or carbon nanomaterials. In a more preferred embodiment, the first electrode comprises carbon nanotubes. These materials can improve biosensor performance by increasing the actual area available for immobilization and facilitating the transfer of charge between the electrode and enzymes.
    In another preferred embodiment, the redox mediator of the charge is selected from among ferrocyanides, ferrocenes, osmium complexes, ruthenium complexes, quinones, phenothiazines or fulvalenes. The redox mediator facilitates the transfer of electrons between the active site of the enzyme and the surface of the electrode on which it (the enzyme) is located.
    In a preferred embodiment, the redox mediator is incorporated into a polymeric structure, such as polyethyleneimines, polyvinyl chlorides, or polymers based on vinylimidazole or vinylpyridine.
    In another preferred embodiment, the electrochromic material is selected from hexacyanomethalates, metal oxides, metallochromic complexes, electrochromic conjugated polymers, viologens or porphyrins.
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    In a more preferred embodiment, the electrochromic material is Prussian blue hexacyanomethalate.
    In another more preferred embodiment, the electrochromic conjugated polymers are selected from polyaniline, polypyrrole, polythiophene, PEDOT, polycarbazole, polyazulene or polyindole.
    The ion conductive medium may be in the form of a membrane, a solution rich in electrolytes, a gel, or in any form that allows ionic exchange between the electrodes. This means is in contact with all the electrodes, and its mission is to house both the supporting electrolyte that allows to control the internal resistance of the device, as well as the sample to be analyzed. In addition, in the case of laminar flow membranes, these allow the exchange of the solutions used easily and comfortably. In a preferred embodiment, this ion conducting medium comprises Nafion, chitosan, polyethyleneimine, agar gels or polyhema.
    In the biosensor of the invention, the enzyme can be an oxidase, dehydrogenase, tyrosinase, etc. In a preferred embodiment, the enzyme is selected from glucose oxidase, glucose dehydrogenase, lactate oxidase, urate oxidase or cholesterol oxidase.
    The immobilization of the enzyme on the surface of the anode can be covalent or non-covalent.
    In another preferred embodiment, the cathode transparent material is selected from ITO, FTO, PEDOT, or materials based on metallic nanowires such as silver.
    In another preferred embodiment, the biosensor of the invention additionally comprises a permeable support that is selected from a non-woven textile material or a non-permeable polymer.
    In another preferred embodiment, the biosensor of the invention additionally comprises an adhesive layer.
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    In another preferred embodiment, the biosensor of the invention additionally comprises a protective layer formed by a transparent polymer.
    In a preferred embodiment, the first electrode functions as an anode and the second electrode functions as a cathode, so that in the anode the reaction of the substrate analyte of the enzyme that generates a current of electrons that are directed by the external circuit towards the external circuit occurs. cathode, where they reduce the electrochromic material and cause its color change.
    In another preferred embodiment, the first electrode functions as a cathode and the second electrode functions as an anode, so that in the cathode there is a reaction of the substrate analyte of the enzyme that induces the passage of an electron current from the anode, where the Electrochromic material changes color when oxidized.
    In the present invention, the electrochemical nature of three of the key components in the detection systems is used, such as the sensor, the power supply, and a possible electrochromic display, fusing the three elements in a single galvanic cell that complies with the time the functions of detection and quantification and visualization of the result. The system works based on a load measurement, since the load that is generated or consumed in the sensor electrode, is matched by another load of equal magnitude and opposite sign on the display electrode. The color change is directly proportional to the circulating load and, through a specific geometric design, it is possible to transform the color change into information through a visual scale. This allows the user to obtain a direct reading of the analyte concentration present in the sample, without the need to use readers or other external instrumentation.
    This approach is completely new from the point of view of the analysis devices for attention points for several reasons. First, it completely eliminates the need for both a silicon-based control electronics, as well as an additional energy source. In addition, the devices based on the technology of the invention can be manufactured entirely by means of printing techniques, using biodegradable materials, thereby not only reducing manufacturing costs, but also increasing the
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    sustainability of the process since the environmental impact of the devices once discarded is greatly reduced.
    The complete elimination of the need to have silicon electronics in a device that contains it means an unprecedented advance in the area of self-powered sensors, by eliminating a barrier unthinkable until now: the heterogeneous integration of electronic components, including integrated circuits usually used for energy management and control of the sensors and the display. The biosensor applications of the invention would be innumerable in medicine (glucose measurement in diabetic patients) and especially advantageous in non-invasive formats such as skin patches for the measurement of sweat metabolites. Other applications are as labels for the detection of pathogens in packaged foods, alcohol meters in saliva, respiration, sweat and alcoholic beverages in general, gas detectors, and even devices for the rapid detection of pathogens and toxic substances in resourceless environments. In general, the technology is applicable to the detection of any substance capable of acting as a substrate for enzymes that can be coupled to an electrode.
    Another aspect of the invention relates to a device comprising the biosensor as described above.
    The device containing the biosensor of the invention has three distinct parts: on the one hand, the sample chamber, in which said biosensor is housed, the display chamber containing an electrolyte and a modified transparent electrode with a layer of electrochromic material, and thirdly the external circuit that connects both electrodes. The device effectively functions as a battery in which the analyte itself is the fuel and therefore serves to activate the device. Analogously to other electrochemical devices for power generation, for the present device to function properly, it is necessary that the potential at which the oxidation reaction in the anode occurs is less than the potential at which the reduction reaction takes place in the cathode. Although in the present invention the anode fulfills the functions of sensor and the display cathode, this configuration is not exclusive, and there could be cases in which the detection reaction occurs in the cathode and the visualization function is
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    Attribute to the anode. The arrangement of the sensor with respect to the display can be such that it is slightly displaced in the XY plane with respect to the transparent electrode containing the electrochromic material, or coplanar form of the sensor and the transparent electrode on the same substrate.
    Throughout the description and claims the word "comprises" and its variants are not intended to exclude other technical characteristics, additives, components or steps. For those skilled in the art, other objects, advantages and characteristics of the invention will be derived partly from the description and partly from the practice of the invention. The following examples and figures are provided by way of illustration, and are not intended to be limiting of the present invention.
    BRIEF DESCRIPTION OF THE FIGURES
    FIG. 1 Shows the descriptive scheme of the basic device and the parts it comprises.
    FIG. 2 Shows the response of the glucose biosensor (using the enzyme glucose dehydrogenase and tetrethiafulvalene, TTF, as a redox mediator) expressed as normalized current density with respect to the area of the electrode (3x3mm).
    FIG. 3 Shows the response of the Prussian blue electrode electrodeposited on a 3x15mm ITO electrode expressed as a normalized current density with respect to the electrode area. The continuous line shows the response of the newly electrodeposited electrode, while the dotted line shows the response of this same electrode, 24 hours after its electrodeposition, in a 0.1M KCl and 0.1M HCl solution. The scanning speed is 10mV s-1.
    FIG. 4. Show the images of the electrochromic electrode and how it changes color progressively as more charge passes. The dotted lines mark the end of the electrode (left) and the position of the color front at each moment (right).
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    EXAMPLES
    The invention will be illustrated below by tests carried out by the inventors, which shows the effectiveness of the product of the invention.
    Example 1: glucose measurement
    A biosensor device was manufactured using rapid prototyping techniques, using polymeric materials laminated with adhesive layers. Said device had screen-printed graphite electrodes (working and auxiliary electrode) and silver / silver chloride (pseudo-reference electrode), as well as a 3x15mm transparent ITO electrode. On the latter, a layer of Prussian blue was electrodeposited following a procedure described in the literature (A. A. Karyakin and E. E. Karyakina, Sensors and Actuators B: Chemical, 1999, 57, 268273). The device was mounted using a lateral flow membrane (Whatmann 1 or Fusion 5) between the screen-printed electrodes and the cathode with Prussian blue. For the electrochromic electrode characterization, the system was completed using a 0.1M KCl and 0.1M HCl solution. This electrolyte provides a conductive medium that limits the potential losses of the system, while providing the acidic medium that stabilizes Prussian blue and provides the potassium cations necessary to maintain the reversibility of the electrochromic process. Manufacturing and assembly are detailed below (the elements mentioned can be seen in Figure 1).
    On a plastic substrate (1) a carbon electrode is printed in its allotropic form of graphite, using a commercial ink (2). On said substrate with the printed electrode, a second adhesive material (3) is cut in a way that allows defining the active areas of the electrode, the contact of the electrode, and an area on which a piece of porous material will rest later (6 ). Next, another sheet of adhesive material (4) on which a band of conductive material, in this case copper (5), which will facilitate electrical contact between the transparent ITO electrode (11) has been placed on the previous assembly. ) and the characterization instrumentation used in the study (not shown in Figure 1). Note that the contact electrode (5) can be printed directly on a layer of unique material that replaces the layers (3) and (4). In the assembly described here two layers of material are used because said material has 130-160 microns of
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    thickness, but the lateral flow membrane (6) can be between 100 and 500 microns thick. After placing the piece represented by (4), the piece (6) is placed. This piece comprises two parts: on the one hand it has a narrow and elongated end (7), and on the other hand it is wider (8). The narrow side of the strip serves as the zone of addition of the sample and also as a zone of separation between the electrodes of the system. The internal resistance of the device is controlled by adding solutions of different saline concentration. The wide side of the strip serves as an absorbent, and allows the added sample on the narrow side to fill the system. On this wider side it is possible to place an additional absorbent that allows the establishment of a continuous flow along the strip. Finally, the transparent chip (9) is placed with the transparent electrode (10) on which a thin layer of Prussian blue has been electrodeposited in an area of 3x15mm (11). After assembly, the biosensor is slightly displaced in the XY plane with respect to the transparent electrode containing the electrochromic material.
    Before placing the lateral flow membrane (6), the graphite electrode is modified to transform it into a glucose biosensor. For this, on the screen-printed carbon electrode, an aqueous mixture is deposited containing an enzyme whose substrate is glucose, a redox mediator, a carbon nanomaterial, a crosslinking agent, and a polymer matrix. The enzyme can be a glucose oxidase or a glucose dehydrogenase in concentrations of the order of 1-50mg mL "1, the redox mediator can be an iron, ruthenium complex or, in a preferred configuration, a polymer loaded with osmium with potential of oxidation near 0V vs. Ag / AgCl In this case, tetrathiafulvalene, TTF, an organic redox compound with an oxidation-reduction potential close to 50mV vs. Ag / AgCl has been used. The cross-linking agent used in this case it was glutaraldehyde at a concentration of 1-1.5% by volume, and the carbon nanomaterial carbon nanotubes at a concentration of 1-10mg / mL. This mixture is deposited by a drop that is allowed to dry. response to glucose concentration of the type shown in Figure 2. Said figure shows the current response of a biosensor as described, measured at a fixed potential such that the oxidation of the mediator is not limited by phenomena of transfer of Charge associated with the electrode, but it depends directly on the activity of the enzyme which, in turn, depends on the concentration of glucose in the medium. If the
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    current in a time interval is obtained the load that has circulated through the biosensor in said time interval. The load measurement in this way constitutes an electrochemical technique known as coulometry. Coulometry has a number of advantages over amperometry, such as greater sensitivity, which allows for smaller concentrations of analyte to be measured than with amperometric methods. In addition, the constant potential load measurements allow a high degree of precision to determine the concentration of an analyte present in a sample even if the diffusion coefficient of the analyte in question is unknown. The total charge (at infinite time) is only a function of the amount of analyte present and the number of electrons involved in the process that takes place to the measurement potential, according to the equation:
    Q = nFNo = nFVCo * (0) Eq. one
    Where Q is the charge measured in coulombs, n the number of electrons involved, F the Faraday constant (96485 Culombios mol-1) and NOT the number of moles of O initially present in the sample. V is the sample volume and CO * the concentration of O initially present in the sample. At shorter times, the load is directly proportional to the amount of initial analyte. Thus, in the proposed biosensor, the coulometric measurement makes special sense since it is intended to analyze the glucose content in a very small sample volume. Therefore, since the load is a cumulative parameter, it is possible to amplify the signal and improve the sensitivity of the device.
    On the other hand, the charge circulating through the biosensor is equalized on the opposite electrode by reducing an electrochromic material homogeneously deposited on the surface of a transparent electrode of a mixed indium and tin oxide (ITO) . In order to manufacture the ITO electrode of the present embodiment, an ITO layer of moderate conductivity (frame resistance less than 15 ohms) and high transparency was deposited, deposited by the chemical vapor deposition technique (CVD) of the acronym of "Chemical Vapor Deposition") on 1mm thick glass wafers. These wafers were cut into 20x20mm dice, and subsequently the electrodes were manufactured in each of the chips. The electrodes were defined in the chips by Recorded
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    wetting of unwanted material in a bath of regal water diluted at room temperature. Electrodes with rectangular shape of dimensions 3x15 mm were prepared, connected to a connection area of the same size by means of a 1mm wide track. This track is important because it helps control the internal resistance of the device.
    Once the electrode was manufactured, the electrochromic material was electrodeposited, which in this embodiment is Prussian blue. The electrodeposition method in this case is amperometry without forced agitation, in a beaker, and polarizing the ITO electrode at 0.4V vs. Ag / AgCl within a 0.1M HCl solution, 2mM ferric nitrate, and 2mM ferricyanide. The method follows the procedure described by Karyakin in (A. A. Karyakin and E. E. Karyakina, Sensors and Actuators B: Chemical, 1999, 57, 268-273). The auxiliary electrode is designed so that the current distribution during the electrodeposition is homogeneous and is not prevented by the presence of the reference electrode. The electrodeposition solution may be deoxygenated. The total applied load is between 1 and 2 mC, and the resulting material has a reduction potential from 0.25 V vs. Ag / AgCl.
    Once the Prussian blue is electrodeposited, the electrode is extracted from the solution rich in iron species and is conditioned, by means of cyclic voltammetry, in a solution of HCl and KCl at pH = 1. The potential is swept between 0 and 0.5 V vs. Ag / AgCl until the oxidation and reduction signals stabilize (remain constant). Figure 3 shows the typical response of the Prussian blue electrode used in the present embodiment. After stabilization of Prussian blue, the electrode is air dried and the system that includes the biosensor described above is assembled.
    A lateral flow membrane is placed on the biosensor, which will allow controlling the vertical separation between the biosensor electrode and the electrochromic electrode, as well as the internal resistance of the system when a pH buffer electrolyte solution is introduced, such as a mixture of salts phosphate, with the sample. After the placement of this membrane, the electrochromic electrode is mounted, which has a contact to be able to connect, through an external circuit, to the
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    biosensor electrode. In the present embodiment, the biosensor and electrochromic electrode are slightly displaced in the horizontal plane, and separated vertically by the membrane. However, in another preferred embodiment, both electrodes could be coplanar, which would simplify the construction of the device. In the case of the present embodiment, the external circuit is a potentiostat that allows characterizing the system. In another embodiment, the external circuit may simply consist of a series of resistors connected in series with a switch that allows both electrodes to be connected when the measurement is to be initiated. Said resistors can be conductive tracks or wires, depending on the application.
    When a sample is added to the described system and the external circuit is closed, the system generates an electromotive force (EMF) around 250mV. This EMF is derived from the reduction potential of the electrochromic material (0.25V) and the oxidation potential of the mediator used in the biosensor electrode (in this case close to 0V). Although this battery potential is insufficient to power virtually any electronic component by itself, the color change produced in the electrode is already directly proportional to the analyte concentration. Thanks to the electrode geometry that contains the electrochromic material and its distribution over the ITO electrode, as well as the relative position between the two electrodes, it is possible that the color change occurs along the electrode, obtaining an indication Direct visual of the glucose concentration present in the sample. On the other hand, the color change is a property of the electrochromic material, so that all the electrical energy produced by the device is also available. Energy that, on the other hand, be used to power other additional functions.
    Example 2: Demonstration of the principle of operation
    To show the principle of operation more clearly, an experiment was designed in which the biosensor electrode was replaced by a current injector electrode. This has the advantage that it allows to precisely control the level of circulating current, as well as the electrical charge that the device passes through. The experimental setup is identical to that of example 1, with the exception that no mixture of redox compounds or enzymes is deposited on the graphite electrode. In this configuration, the graphite electrode is connected to the working electrode of the potentiostat, while the transparent electrode containing Prussian blue is
    connects to the terminals of the auxiliary electrode and the reference. 10 ^ A currents are passed through the system, and images of the device are taken over time. Figure 4 shows 3 key images in which it is appreciated that the color change occurs progressively along the electrode as a function of the circulated charge 5, and not homogeneously on the entire surface thereof. The fact that the color change occurs progressively along the electrode indicates that the circulating current preferentially passes through the path of least internal resistance at all times. As the Prussian blue closest to the graphite electrode has oxidized, it changes color. If current continues to circulate, the electrochromic material will continue to be transformed, always following the path of least resistance along the electrode, until completely exhausted. Given the geometric design of the system, this transformation occurs along the electrode, allowing the quantification of the circulating load visually, and directly without the need for additional electronics. The amount of transformed Prussian blue 15 corresponds directly to the circulated electric charge. Therefore, by the controlled deposit of electrochromic material on the electrode, it is possible to have an idea of the charge per unit area present in the electrode, and therefore the area affected by the color change can be used as a direct indicator of the analyte concentration present in a sample.
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    权利要求:
    Claims (17)
    [1]
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    1. Biosensor that includes the following elements:
    a) a first electrode formed by a base of a conductive material comprising at least one type of enzyme immobilized on its surface and at least one redox mediator of the charge;
    b) a second electrode formed by a transparent material on which an electrochromic material has been deposited;
    c) an ion conductive medium, located between both electrodes.
    [2]
    2. Biosensor according to claim 1 wherein the first electrode further comprises graphene or carbon nanotubes.
    [3]
    3. Biosensor according to any of the preceding claims wherein the redox mediator of the load is selected from ferrocyanides, ferrocenes, osmium complexes, ruthenium complexes, quinones, phenothiazines or fulvalenes.
    [4]
    4. Biosensor according to any of the preceding claims wherein the redox mediator is incorporated into a polymer structure.
    [5]
    5. Biosensor according to the preceding claim wherein the polymer structure is selected from polyethyleneimines, polyvinyl chlorides, polymers based on vinylimidazole or polymers based on vinylpyridine.
    [6]
    6. Biosensor according to any of the preceding claims wherein the electrochromic material is selected from hexacyanomethalates, metal oxides, metallochromic complexes, electrochromic conjugated polymers, viologens or porphyrins.
    [7]
    7. Biosensor according to the previous claim where the electrochromic material is Prussian blue.
    [8]
    8. Biosensor according to claim 6 wherein the electrochromic conjugated polymers are selected from polyaniline, polypyrrole, polythiophene, PEDOT, polycarbazole, polyazulene or polyindole.
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    [9]
    9. Biosensor according to any of the preceding claims wherein the ion conducting medium comprises Nafion, chitosan, polyethylenediamine, agar gel or polyhema.
    [10]
    10. Biosensor according to any of the preceding claims wherein the enzyme is selected from glucose oxidase, glucose dehydrogenase, lactate oxidase, urate oxidase, cholesterol oxidase.
    [11]
    11. Biosensor according to any of the preceding claims wherein the transparent material of the second electrode is selected from ITO, FTO, PEDOT, or metallic nanowires.
    [12]
    12. Biosensor according to any of the preceding claims which additionally comprises a permeable support that is selected from a nonwoven textile material or a non-permeable polymer.
    [13]
    13. Biosensor according to any of the preceding claims which additionally comprises an adhesive layer.
    [14]
    14. Biosensor according to any of the preceding claims which additionally comprises a protective layer formed by a transparent polymer.
    [15]
    15. Biosensor according to any of the preceding claims wherein the first electrode functions as an anode and the second electrode functions as a cathode.
    [16]
    16. Biosensor according to any one of claims 1 to 14 wherein the first electrode functions as a cathode and the second electrode functions as an anode.
    [17]
    17. Device comprising the biosensor according to any of claims 1 to 16.
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    同族专利:
    公开号 | 公开日
    ES2623080B1|2018-05-14|
    WO2017098076A1|2017-06-15|
    引用文献:
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    WO2013130145A2|2011-11-30|2013-09-06|The Regents Of The University Of California|Printed biofuel cells|WO2019189808A1|2018-03-29|2019-10-03|東洋紡株式会社|Nanocarbon electron transfer action|
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    CN109369974B|2018-11-08|2021-03-30|西北师范大学|Preparation method of reduced graphene oxide-ferrocene-chitosan composite material|
    CN109541005B|2018-12-12|2021-03-30|上海交通大学|Uric acid visual measurement method based on mobile reaction interface electrophoresis titration chip|
    ES2781376B2|2019-02-28|2021-01-27|Consejo Superior Investigacion|Wireless, portable and reusable biosensor|
    CN110632147B|2019-09-27|2021-06-08|南京工业大学|Preparation method of efficient sensing electrode of lactic acid sensor|
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    ES201531788A|ES2623080B1|2015-12-10|2015-12-10|ELECTROCROMIC BIOSENSOR|ES201531788A| ES2623080B1|2015-12-10|2015-12-10|ELECTROCROMIC BIOSENSOR|
    PCT/ES2016/070873| WO2017098076A1|2015-12-10|2016-12-09|Electrochromic biosensor|
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