• 专利附录
  • 专利说明
  • 权利要求
  • 类似技术
  • 同族专利
  • 引用文献
  • 法律状态
  • 优先权
    • 专利摘要:
    OPTICAL DEVICE, TESTING EQUIPMENT, SAMPLE SUPPORT AND TEST METHOD. An optical device (22) includes a housing (30), which fits over at least part of a mobile computing device (24) which has the first and second faces and includes a light source (29), which emits a beam of illumination through an exit opening, and a camera module (27), which captures images through an entrance opening. Both the entry opening and the exit opening are opened through the second face of the mobile computing device. A receptacle (31) in the housing receives and positions a sample (56) in the vicinity of the second face of the mobile computing device within a field of view of the camera module. The illumination optic (36, 38) receives and deflects the beam emitted by the light source in such a way as to backlight the sample while the camera module captures one or more images of the backlit sample.
    公开号:BR112016011252B1
    申请号:R112016011252-0
    申请日:2014-12-09
    公开日:2020-11-24
    发明作者:Assaf BEN SHOSHAN；Vitaly STRONGIN；Lior SHRIKI；Marcia DEUTSCH
    申请人:Mes Medical Electronic Systems Ltd；
    IPC主号:
    专利说明:

    Cross-referencing related orders
    [001] The present application claims the benefit of the North American provisional patent application 61 / 914,980, filed on December 12, 2013, which is incorporated herein by reference. Field of invention
    [002] The present invention relates in general to the optical inspection of samples and, specifically, to automated devices and methods for the capture and analysis of electronic images of samples. Foundation
    [003] Infertility is a complex but common problem that affects approximately 10 to 15% of couples trying to conceive a child. In up to a third of these cases, the problem is, at least in part, related to male reproductive problems. Among these issues, low sperm motility is commonly associated with infertility. The qualitative evaluation of the mobile sperm can be done through the visual evaluation of the movement of the sperm in the sample under the microscope. These microscope-based systems are generally expensive, however, which is not suitable for use by inexperienced people. This type of visual assessment can produce inconsistent results, even when used by well-trained personnel.
    [004] Several devices and methods have been developed for automated sperm motility testing. For example, the publication of the North American patent application 2014/0254004 describes test kits to assess male fertility, which includes a sample holder that defines an object plane, a lens, and a two-dimensional light sensor that defines a plane image arranged along a common linear axis. The test kit can have a housing with a maximum linear dimension of no more than 100 mm. The processing circuit can be predicted, which is configured for the production of a sperm count and / or the measurements of the mnt-ilidadp dnc pcnprmatnvnidoc nnr mpin dn nrnrpccampntn of the dadnc dn magnet two-dimensional light sensor.
    [005] Almost all mobile phones currently on sale include built-in cameras, which can be used in various applications. For example, US patent application publication 2011/0292198 describes a microscope accessory for attachment to a mobile phone that features a viewfinder positioned on one first face and a camera positioned on a second opposite face. The microscope accessory includes latching features for removably connecting the microscope accessory to the mobile phone, and an optical assembly that features a first mirror positioned to be moved from the camera, a second mirror positioned for alignment with the camera, and a microscope lens positioned in the optical path. The optical assembly is combined with the camera, in such a way that the surface is in focus when the mobile phone rests flat against the surface. Synthesis
    [006] Some of the embodiments of the present invention that are described hereinafter provide devices and methods that make use of the imaging and processing capabilities of a mobile computing device, such as a Smartphone, to perform automated assessment and measurement. microscopic inspection of a sample.
    [007] Therefore, according to an embodiment of the present invention, an optical device, including a housing, is provided, which is configured to fit over at least part of a mobile computing device having a first and second face and including a light source, which emits a beam of light through an exit opening, and a camera module, which captures images through an entrance opening, with both the entrance opening and the exit opening being opened through the second face of the mobile computing device. A receptacle in the housing is configured to receive and position a sample in proximity to the second face of the mobile computing device within a field of view of the camera module. The lighting optics are configured to receive and transform the beam emitted by the light source in order to backlight the sample while the camera module captures one or more images of the sample.
    [008] In the described embodiments, the light source directs the beam away from the second face of the mobile computing device, and the lighting optics includes at least one reflector, which is configured to reflect the beam back for the second face. The device may include a pair of reflective surfaces, each configured to deflect the beam at 90 °.
    [009] In some embodiments, the device includes a lens mounted in the housing, in a location between the sample and the inlet aperture and configured in such a way that the sample is located within a focal length of the camera module. Typically, the lens is configured to enlarge one or more images captured by the camera module. In a described embodiment, the lens includes a spherical lens, which has wings attached to it for mounting in the housing.
    [0010] In some embodiments, the apparatus includes a transparent sample holder, which is configured to receive the sample and to be positioned inside the receptacle, in order to firmly position the sample in the camera's field of view. When the sample is liquid, the sample holder usually includes a slide that has a depression formed in it to hold the sample and a slide fixed over the depression.
    [0011] Also provided, according to an embodiment of the present invention, the test apparatus, including a mobile computing device that has a first and second face and including a light source, which emits a beam of light through an exit opening, and a camera module, which captures images through an entrance opening, with both the entrance and exit openings being opened through the second side of the mobile computing device. The apparatus further includes an optical device, as described above, which is configured to fit over the mobile computing device so that the positions of the sample receptacle within the field of view of the camera module, and the optics of lighting, are aligned with the light source.
    [0012] In a described embodiment, the mobile computing device is a Smartphone.
    [0013] Typically the mobile computing dipositivi includes a processor which is configured to process the captured images in order to analyze a sample property. In some embodiments, the sample includes semen, and the processor is configured to analyze a sperm motility characteristic in the semen.
    [0014] In addition or alternatively, the mobile computing device includes a display screen on the first face of the mobile computing device, and the processor is configured to present a property assessment on the display screen.
    [0015] Additionally, in accordance with an embodiment of the present invention, a sample holder is provided, including a slide, which contains a depression in one surface of the slide, and a slide, which is fixed on the slide over the depression, in order to define a sample chamber, while leaving a cargo area of the depression uncovered, so that a liquid sample deposited in the cargo area is dragged, that is, forced to move to the sample chamber by capillary action .
    [0016] In one described embodiment, the blade is shaped to define at least one first groove, in which an adhesive is inserted, in order to fix the lamella on the blade, and at least a second groove located between at least one first groove and the depression, in order to avoid overflow of the adhesive from at least one first groove in the sample chamber.
    [0017] According to an embodiment of the present invention, a method for testing is also provided, which includes inserting a sample into a transparent sample holder and inserting the transparent sample holder with the sample within an optical adapter. The optical adapter is fitted over a mobile computing device that includes a light source, which emits a beam of light through an exit opening, and a camera module, which captures images through an entrance opening, so that the sample is positioned within a field of view of the camera module. An image of the sample on the optical adapter is captured using the camera module while illuminating the sample with the beam emitted by the light source. The captured image is analyzed on the mobile computing device in order to allow the evaluation of the sample.
    [0018] In some embodiments, the sample is liquid, and the sample holder includes a slide equipped with a depression formed in it to contain the sample and a fixed slide on the depression. In one described embodiment, the slide is attached to the slide over the depression in order to define a sample chamber, while leaving a loading area of the depression uncovered, and inserting the sample includes depositing the sample in the loading area so that the liquid is dragged into the sample chamber by capillary action.
    [0019] In a described embodiment, the mobile computing device is a Smartphone, and the analysis of the captured image includes processing the captured image using an application software running on a processor on the Smartphone
    [0020] In some embodiments, the sample includes semen, and the analysis of the captured image includes a motility characteristic of the sperm in the semen, for example, by calculating a concentration of mobile sperm.
    [0021] The present invention will be more fully understood from the following detailed description of the embodiments thereof, taken in conjunction with the drawings, in which: Brief description of the drawings
    figure 1 is a schematic, pictorial illustration of the test apparatus, according to an embodiment of the present invention; figures 2A and 2B are exploded schematic views of the apparatus of figure 1, shown from the front and rear sides of the apparatus, respectively, in accordance with an embodiment of the present invention; figure 3 is an exploded schematic view of a microscopy adapter for a mobile computing device used in the apparatus of figure 1, according to an embodiment of the present invention; figure 4 is a schematic cross-sectional view showing details of the apparatus of figure 1, according to an embodiment of the present invention; -Figure 5 is a schematic front view of a test slide, according to the image Ho roali7arãn Ha nrpcpnfp invAnrSrv -Figure 6 is a flowchart that schematically illustrates a method for sperm testing, according to a embodiment of the present invention; and figure 7 is a flowchart that schematically illustrates a method for the automatic assessment of the concentration of mobile sperm, according to an embodiment of the present invention. Detailed description of the embodiments
    [0022] In view of the high incidence of male fertility problems, sperm tests should be performed at an early stage in most cases where a couple has difficulty conceiving. This type of testing, however, requires that the man ejaculate a semen sample in the laboratory, which can be difficult and embarrassing, or run from your home to the laboratory with the sample, with the risk that the sperm may die during the traffic.
    [0023] The embodiments of the present invention that are described herein solve this problem by providing an automated sperm tester that can be operated conveniently and reliably in the privacy of your home. The device comprises an optical adapter, which fits into an existing mobile computing device, such as a smartphone, and converts the device into a video microscope. The device takes advantage of the existing features of the mobile computing device, including camera (and its internal lenses), light source, processor and display screen, and therefore provides a low-cost solution that allows the user to test their semen on home at any stage, even before going to a doctor.
    [0024] In the described embodiments, a pluggable optical adapter device comprises a housing, which fits over at least part of a mobile computing device, such as a smartphone, where the light source and the camera module are located. The housing of the adapter device has a sample receptacle, such as a semen sample, or other liquid sample, contained in an appropriate sample holder, such as a clear slide. When inserted into this receptacle, the sample is positioned within the field of view and a final dictation of the camera's location in the center of the mobile computing dicnncifivn in which the entry and exit openings are located. In the described embodiments, the desired focus and sample enlargement are achieved with the help of the internal optics of the adapter device, as described below.
    [0025] To capture a suitable electronic image of the sample for analysis, it is desirable that the sample be backlit. On all common smartphones, as well as other similar devices, however, the light source outlet opening and the camera module inlet opening are located on the same face of the device, not facing each other, as needed for backlight. A separate light source can be envisaged for this purpose, but this approach increases the size and cost of the adapter and also requires that you supply power to the light source.
    [0026] Instead, the described embodiments of the present invention exploit the light source existing in the mobile computing device itself, using the lighting optics in the adapter device housing to receive and deflect the beam emitted by the light source from to backlight the sample. Thus, the camera module (with the aid of the adapter device's optics) captures the backlit images of the sample, which are processed by the mobile computing device in order to process and issue an evaluation of the sample. Typically, the adapter device also comprises a lens mounted in the housing, in a location between the sample and the inlet opening of the camera module, which enlarges the images captured by the camera module.
    [0027] A pluggable adapter of this type, which takes advantage of the light source and the camera module in a Smartphone or other mobile computing device, can be useful in several methods for automated tests. Thus, the embodiments of the present invention also provide a method in which a sample is inserted into a transparent sample holder, the transparent sample holder with the sample is inserted into an optical adapter, and the optical adapter is fitted over a device mobile computing. (Alternatively, the adapter can be placed first on the mobile computing device, after which the holder of the sample P incpridn nn adantadnA The sample of the sample chamber, which is illuminated by the beam emitted by the light source An application program running on the mobile computing device acts on the camera module and the light source, and causes the processor on the device to analyze the images in order to process and issue an evaluation of the sample.
    [0028] In one described embodiment, the sample comprises semen, as mentioned above, and the processor assesses the motility of the sperm in the semen. Advantageously, the processor can process and issue an evaluation of a sperm motility characteristic, such as sperm motility concentration (MSC, also referred to in some publications as the sperm motility count), which is the product sperm concentration multiplied by the percentage sperm motility (divided by 100). Because the MSC integrates these two parameters, a better indication of screening for fertility problems results than any concentration or motility of sperm alone.
    [0029] Figure 1 is a schematic, pictorial illustration of the tester 20, according to an embodiment of the present invention. The apparatus 20 comprises a mobile computing device in the form of a Smartphone 24, which can be of any suitable type, which is known in the state of the art. An optical adapter device 22 fits over the top of Smartphone 24. A sample holder, in the form of a test slide 26, is inserted into the adapter device, so that the sample it contains is positioned within the field of view and focal length of the camera module on Smartphone 24 (as shown in the following figures, which include the internal optics on adapter device 22).
    [0030] The processor (not shown) on Smartphone 24 analyzes the images captured by the camera module in order to make an evaluation of the sample, and issues the evaluation to a display screen 28. The images captured through the camera module are referred to here indifferently as "electronic images" or "video images". For the sake of clarity in the description that follows, the term "first face" in reference to Smartphone 24 refers to the side of the Smartphone on which screen PYihirãn 7R CP Inrali7a PMiianfn miP a farp nnncfa wrJfarla nara haivn in fineness 1 preferred as the second face.
    [0031] Despite the present embodiment and the figures in this patent application refer to a particular type of Smartphone, the characteristics of this embodiment can be adapted, once the necessary changes have been made, to operate with smartphones of other types and models, as well as with other mobile devices that have adequate imaging and processing capabilities, such as laptop computers and tablets. All of these alternative implementations are considered to be within the scope of the present invention.
    [0032] Figures 2A and 2B are exploded schematic views of the apparatus 20, shown from the front and rear sides, respectively, according to an embodiment of the present invention. As noted earlier, figure 2A shows the "first face" of Smartphone 24, while figure 2B shows the "second face". The Smartphone comprises a camera module 27 and a light source 29, which respectively have an inlet and an outlet opening side by side on the second side of the Smartphone. The adapter device 22 fits over the end of the Smartphone 24 and covers the inlet and outlet openings of the camera module 27 and light source 29.
    [0033] The test slide 26 fits inside a receptacle 31, which is shaped like a slot in the housing of an adapter device 22. Alternatively, the receptacle 31 can be configured to receive the samples and the sample holders, made appropriate transparent materials of any suitable size and shape.
    [0034] Figure 3 is an exploded schematic view of the adapter device 22, according to an embodiment of the present invention. The device 22 comprises a housing 30, which is typically made of injected plastic and includes a slot for the receptacle 31. The springs 32 are mounted on the housing in order to hold the blade 26 safely and precisely in the receptacle, with the sample in the field of view and focal length of the camera module 27. A mirror holder 34 fits into the rear of the housing 30 and contains a pair of mirrors 36 and 38, whose operation together rnm nnfira dn ihiminarãn nn dicnncifiwn 7 P dpcrrita ahaivn rnm rpfprpnria fineness 4
    [0035] A spherical lens 40, retained between a pair of lens holders 42, is mounted on the housing 30 at a location between the sample held by the blade 26 and the inlet opening of the camera module 27. The springs 32 retain the blade 26 at a fixed, precise distance from lens 40. Lens 40 serves to enlarge the sample images that are captured through the camera module. The lens supports 42 are shaped like wings, which extend outwardly to both sides of the spherical lens 40, without occlusion of the small optical aperture of the spherical lens. Alternatively, the spherical lens and the wings can be injected together into a single piece of plastic or optical glass. Typically, the spherical lens 40 is about 2 mm in diameter, although alternatively larger or smaller elements can be used. Alternatively, the spherical lens 40 can be replaced by other magnifying optics, such as a simple miniature lens (either spherical or aspherical) or even a composite lens, depending on the desired magnification and other optical requirements.
    [0036] Figure 4 is a schematic sectional view showing details of the apparatus 20, and particularly of the adapter device 22, according to an embodiment of the present invention. This figure illustrates the functionality of the elements that have been described above with reference to figure 3. Light source 29 directs a beam 54 of illumination beyond the second face of Smartphone 24. The beam 54 is reflected back to the entrance opening of the camera module 27 on the second side of the Smartphone through the reflective surfaces of mirrors 36 and 38, each of which deflects the beam by 90 °. In the illustrated embodiments, mirrors 36 and 38 comprise reflectors facing the surface. Alternatively, one or more reflectors of a different shape, such as a suitable prism with reflecting faces, or even transmissive optics, such as a curved light guide, can be used in place of mirrors 36 and 38.
    [0037] Following the reflection of mirrors 36 and 38, beam 54 backlit a sample 56 held by blade 26. The spherical lens 40 creates an enlarged image of the sample in the image sensor plane in the camera module 27. The Smartphone 24 captures and processes the image, as described below.
    [0038] with an embodiment of the present invention. The slide 26 comprises a transparent plastic or glass, which is injected or otherwise manufactured to define a depression 58, typically about 100 µm deep, on the surface of the slide, where the liquid sample is to be held. For example, the depression can alternatively be formed by depositing a layer of an appropriate double-sided adhesive, about 100 µm thick, on the surface of the slide around the area that is to hold the sample. A transparent slide 60, typically about 0.3 mm thick, is attached to the slide over the depression (whether molded or formed by means of double-sided adhesive or other means) in order to define a sample chamber, which is aligned with the spherical lens 40, as shown in the figure. The slide 60 is positioned to leave a loading area 62 of the depression 58 uncovered. Consequently, when a liquid sample is deposited in the loading area 62, the liquid is drawn into the sample chamber by capillary action. The slide 26 can then be loaded into the receptacle 31 for imaging the sample. The shape of the slide 26 ensures that a controlled, known volume of semen is positioned in the field of view of the camera module 27, thus facilitating reliable imaging and evaluation.
    [0039] Typically, the lamella 60 is glued to the lamina 26. For this purpose, the lamina can be shaped to define one or more grooves 64, in which an adhesive is inserted before applying the lamella to the lamina. Once cured (for example, through ultraviolet lighting), the adhesive fixes the coverslip to the blade. To avoid overflow of glue from the grooves 64 into the sample chamber, one or more grooves 66 can be molded in places between the grooves 64 and the depression 58. Alternatively, as indicated above, the coverslip can be placed on the top of the shaped double-sided adhesive that creates the depression 58.
    [0040] The shape of the slide 26 and its dimensions are shown and described here as an example. In alternative embodiments (not shown in the figures), adapter device 22 can accommodate sample holders of other shapes and dimensions.
    [0041] the sperm test according to an embodiment of the present invention. The method is described, for reasons of convenience and clarity, with reference to the apparatus 20, but can also be applied using mobile computing devices with other types of adapters and samples.
    [0042] The user begins the procedure with the preliminary step of installing the test application software on Smartphone 24. The software can be downloaded from a website or "virtual application store" [app store], as it is known in the state of art. It includes a user interface, which guides the user through the test and receives user input as needed, as well as the drive components for operating camera module 27, light source 29, and display screen 28 , as necessary. The application software also includes the image processing and analysis components, which analyze the sample images that are captured by the camera module and process the test results.
    [0043] Once the application has been installed, the user prepares and collects a semen sample, in a sample preparation step 70. For this purpose, the user can mix the semen with a liquefying agent, such as chymotrypsin , to reduce its viscosity. The user inserts a small amount of the sample into the loading area 62, in a sample transfer step 72, after which the sample is dragged into the chamber defined by a depression 58, in a sample transfer step 72. For For example, the user can pour a small amount of the liquefied semen into a capillary tube, and then place the end of the capillary tube in the loading area so that the semen fills the sample chamber.
    [0044] The user inserts the slide 26 with the sample into the receptacle 42 of the optical adapter device 22, so that the slide is held firmly in place, in a step of inserting the slide 74. The user then fixes the device 22 on the end of the Smartphone 24, as shown in the previous figures, in a fixing step of the device 76. Alternatively, the order of steps 74 and 76 can be reversed. In both cases, the sample is ready for imaging.
    [0045] is in position by pressing a control shown on screen 28, in a measurement triggering step 78. This step causes the application to activate the light source 29 and operate the camera module 27 to capture one or more images from the sample. The image processing component of the application causes the processor 24 on the Smartphone to process the electronic images on the mobile computing device in order to process and issue an evaluation of the sample. The processing performed in this step is described in more detail below with reference to figure 7.
    [0046] Upon completion of the processing of the captured images, the Smartphone 24 outputs the results, typically through a display screen 28, in a data emission step 80. The measurement results, such as the MSC, can be displayed as a numeric value. Alternatively or additionally, it may be preferable to simply report the interval at which the result fails, in order to indicate to the user on the screen view whether or not there is cause for concern. For example, in the case of MSC, the result can be reported to the user as being "normal" if it is not less than 6 million / ml, or as "low" if the result is less than 6 million / ml. In the latter case, the user may be asked to run the test again, and consult a doctor if the MSC found is low again.
    [0047] Figure 7 is a flowchart that schematically illustrates a method for the automatic evaluation of sperm motility, according to an embodiment of the present invention. This method is initiated when the user initiates the measurement process in step 78 (figure 6). As noted above, in this step, the light source 29 begins to operate, and the TJ camera module captures a sequence of images from the backlit sample, typically in a high resolution setting. Smartphone 24, which operates under the control of the application software mentioned above, expects the resulting video images to stabilize, in a stabilization step 90. Waiting for stabilization is useful due to the Smartphone's auto focus feature requiring time to acquire a optimal video focus while the camera module is operating. The Smartphone processor can simply wait for a tamnn nrp-dpfinirln rnmn 90 cpmindnc in the page QO Fm alf-prnai-iva n nrnnrama application can receive a return from the camera module 27 indicating that the autofocus has been locked and, only at that moment, will start capturing the images for analysis.
    [0048] Within the stable video image, the Smartphone processor chooses the area that presents the sharpest view of the sperm cells for analysis, in a region of the selection stage (ROI) of interest 92. The inventors found that a 700x700 pixel ROI is convenient for analysis and gives reliable results. The processor tries to select an ROI with good contrast and focal quality.
    [0049] For this purpose, for example, the processor can look for the maximum gradient of gray level change in each pixel over time (across all processed image frames) and determine the maximum gradient over all pixels. The processor identifies all the pixels in the captured video stream that had a gradient of at least 50% of the maximum gradient in at least one moment in the image sequence. The ROI is then chosen to be the 700x700 pixel area that is centered on the center of gravity for all identified high gradient pixels.
    [0050] The following application program makes the Smartphone's processor detect the pixels within the ROI that may belong to the sperm cells, in a 94 pixel detection step. The pixels belonging to the sperm cells are defined as dark pixels surrounded by an area of bright pixels. These dark pixels are detected by first setting the brightness of the background environment and areas that are suspected to contain sperm cells, and then this brightness criterion classifies the pixels in the images.
    [0051] The processor aggregates the potential sperm pixels that have been detected in spots, in a 96 pixel aggregation step. This step can use the image processing methods that are known in the art for the identification of connected components. Typically, groups of adjacent sperm pixels are labeled in order to identify them as "spots", and the spots are filtered by size, so that only the spots of a given size are minimized in each area of the area. nprocessor marks the center of each spot that meets these criteria.
    [0052] The processor counts the number of sperm in motion in each image frame by comparing the location of each spot in relation to its location in the previous frame, in a motion counting step 98. If a particular spot is discovered to have if moved, the sperm movement count is increased by one. The total number of spots that have moved, thus, results in the movement count for each frame. The following processor processes the mobile sperm count based on the average moving spot count per image frame, in a motility count step 100. This value is scaled by the semen volume contained in the ROI, in order to result in actual MSC value as defined above. The result is output to screen 28 in step 80 (figure 6).
    [0053] Alternatively, other methods of image processing that are known in the state of the art can be applied in the analysis of the image sequence of the sample captured by the camera module 27. These alternative methods, as well as the method described above, can be applied not only in the processing of MSC, but also in measures of extraction only of motility and / or sperm count, as well as analyzing other qualities of semen and other types of samples.
    [0054] Although the embodiments described above refer specifically to sperm testing and, more particularly, the evaluation of MSC, the principles of the present invention can also be applied to test samples of other types, using the capabilities of existing mobile computing. It will thus be appreciated that the above described embodiments are cited by way of example, and that the present invention is not limited to what has been particularly shown and described above. On the contrary, the scope of the present invention includes both the combinations and sub-combinations of the various resources described above, as well as variations and modifications thereof that may occur to those skilled in the art after reading the previous description and which are not described in the Qctarln da art »
    权利要求:
    Claims (21)
    [0001]
    1. Optical device (22), comprising: -a casing (30), which is configured to fit over at least part of a mobile computing device (24) which has the first and second faces and including a light source ( 29), being that, in use, the light source (29) of the mobile computing device (24), into which the optical device (22) is fitted, is arranged to emit a beam of illumination through an exit opening and direct the beam beyond the second face of the mobile computing device, and a camera module (27), arranged to use images to capture images through an inlet opening, with both the inlet and outlet opening being opened through the second face of the mobile computing device; a receptacle (31) in the housing configured to receive and position a sample (56) in proximity to the second face of the mobile computing device within a field of view of the camera module; and -the lighting optics, configured to backlight the sample while the camera module of the mobile computing device (24) in which the optical device (22) is fitted, captures one or more images of the backlit sample, characterized because the lighting optics comprise a pair of reflective surfaces (36, 38), each configured to deflect the beam emitted by the light source (29) by 90 °, and together reflect the beam emitted by the light source back to the second face of the mobile computing device (24) to which the optical device (22) is attached.
    [0002]
    2. Device according to claim 1, characterized in that it comprises a lens mounted in the housing at a location between the sample and the inlet opening and configured in such a way that the sample is located within a focal distance of the camera module.
    [0003]
    3. Device according to claim 2, characterized in that the lens is configured to enlarge to one or more images captured by the camera module.
    [0004]
    4. Device according to claim 2, characterized in that the lens comprises a spherical lens, which has wings attached to it for mounting nn invnhirrn
    [0005]
    5. Device according to claim 1, characterized in that it comprises a transparent sample holder, which is configured to receive the sample and to be positioned inside the receptacle in such a way as to firmly position the sample in the camera's field of view.
    [0006]
    6. Device according to claim 5, characterized in that the sample is liquid, and in that the sample holder comprises a slide that has a depression formed in it to contain the sample and a slide fixed on the depression.
    [0007]
    7.Test apparatus (20), comprising: -a mobile computing device that has a first and second face and comprises a light source, which emits a beam of light through an outlet opening, and a camera module, which captures images through an entrance opening, characterized in that both the entrance opening and the exit opening are opened through the second face of the mobile computing device; and an optical device defined in any one of claims 1 to 6, which is configured to fit over the mobile computing device in such a way that the positions of the sample receptacle within the field of view of the camera module, and the optics lighting, are aligned with the light source.
    [0008]
    8. Apparatus according to claim 7, characterized in that the mobile computing device is a Smartphone.
    [0009]
    Apparatus according to claim 7 or 8, characterized in that the mobile computing device comprises a processor, which is configured to process the captured images in such a way as to analyze a sample property.
    [0010]
    10. Apparatus according to claim 9, characterized in that the sample includes semen, and that the processor is configured to analyze a motility characteristic of sperm in the semen.
    [0011]
    11. Apparatus according to claim 9, characterized in that the mobile computing device comprises a display screen on the first face of the mnvpl mnvpl or nnr n nrnraccadnr nrnfiniiradn nara anrpcpnn nr anrpcpnn nrr property evaluation on the screen.
    [0012]
    12. Method for testing using the apparatus as defined in claim 7, comprising: - inserting a sample (56) into a transparent sample holder (26); - inserting the transparent sample holder with the sample in an optical adapter (22) as defined in claim 1; -the fitting of the optical adapter in a mobile computing device (24) which includes a light source (29), in use the light source (29) of the mobile computing device (24) in which the device optical (22) is fitted and arranged to emit a beam of illumination through an exit opening, and a camera module (27), arranged to use to capture captures images through an entrance opening, such that the sample is positioned within a field of view of the camera module, -the mobile computing device has the first and second faces, and both the inlet and outlet openings are opened through the second face, the source being of light is arranged to direct the beam beyond the second face of the mobile computing device; - capturing an image of the sample on the optical adapter using the camera module while illuminating the sample; and -analysis of the image captured on the mobile computing device in such a way as to process and issue an evaluation of the sample. characterized in that the optical adapter (22) comprises the illumination optics, which comprises a pair of reflecting surfaces (36, 38), each configured to deflect the beam at 90 °, so that the illumination optics receives and deflects the beam emitted by the light source in such a way as to backlight the sample while the camera module, of the mobile computing device (24) in which the optical device (22) is fitted, captures the image.
    [0013]
    13. Method according to claim 12, characterized in that the capture of the image comprises the application of a lens mounted on the optical adapter in an Inalítarãn antria a amnctra P a ahpr + i will pntrarla rln mndi iln dp rlp camera such a way to form a sample image within a focal length of the camera module.
    [0014]
    Method according to claim 13, characterized in that the application of the lens comprises the enlargement of the image.
    [0015]
    Method according to any one of claims 12 to 14, characterized in that the sample is liquid, and in that the sample holder comprises a slide that has a depression formed in it to contain the sample and a slide fixed on the depression .
    [0016]
    16. Method according to claim 15, characterized in that the lamella is fixed on the slide over the depression in such a way as to define a sample chamber, while leaving a load area of the depression uncovered, and in that the sample insertion comprises the deposition of the sample in the loading area so that the liquid is dragged into the sample chamber by capillary action.
    [0017]
    17. Method according to any one of claims 12 to 14, characterized in that the mobile computing device is a Smartphone.
    [0018]
    18. Method according to claim 17, characterized in that the analysis of the captured image comprises the processing of the captured image using application software running on a processor on the Smartphone.
    [0019]
    19. Method according to any one of claims 12 to 14, characterized in that the sample includes semen, and that the analysis of the captured image comprises the evaluation of a sperm motility characteristic in the semen.
    [0020]
    20. Method according to claim 19, characterized in that the evaluation of the motility characteristic comprises the processing of a concentration of mobile sperm.
    [0021]
    21. Method, according to any one of claims 12 to 14, characterized in that the analysis of the captured image comprises the presentation of the sample evaluation on a display screen of the mobile computing device.
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    公开号 | 公开日
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    JP2017506324A|2017-03-02|
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    MX2016006378A|2016-08-01|
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    CN105793694A|2016-07-20|
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    2020-08-11| B09A| Decision: intention to grant [chapter 9.1 patent gazette]|
    2020-11-24| B16A| Patent or certificate of addition of invention granted [chapter 16.1 patent gazette]|Free format text: PRAZO DE VALIDADE: 20 (VINTE) ANOS CONTADOS A PARTIR DE 09/12/2014, OBSERVADAS AS CONDICOES LEGAIS. |
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