methods of automated selection of microorganisms and identification with the use of curs

专利摘要:
AUTOMATED SELECTION OF MICRO-ORGANISMS AND IDENTIFICATION WITH THE USE OF MALDI. A method and a device for the location and selection of a colony of microorganisms on a culture plate and identification of microorganisms in said selected colony using MALDI. The method comprises the steps of locating and selecting a colony of microorganisms on a culture dish; obtain a sample of said selected microorganism colony; depositing at least some of said sample of said selected microorganism colony on a target plate; and transferring said target plate with said sample to an apparatus for performing MALDI for identification of said sample of said selected microorganism colony. A sample of a colony of microorganisms is automatically deposited at a deposition point so that the sample covers, at most, approximately half of said one of the deposition points of the target plate. A suspension of a sample of microorganisms is automatically prepared by automatically collecting the sample with a selection tool and submersion of the selection tool with said sample in a suspension, after which the (...) tool.
公开号:BR112014024474B1
申请号:R112014024474-0
申请日:2013-04-02
公开日:2020-12-01
发明作者:Jetze Botma；Martijn Kleefstra；Tino Walter Van Der Zee
申请人:Bd Kiestra B.V；
IPC主号:

专利说明:

FIELD OF THE INVENTION
[001] The present invention generally relates to a method for locating and selecting a colony of microorganisms and identifying microorganisms using MALDI, in particular MALDI-TOF-MS (Matrix Assisted Laser Desorption and Ionization Time-of-Flight Mass Spectroscopy) (Time-of-Flight Mass Spectroscopy Matrix with Desorption and Laser Ionization) and the systems for carrying out such methods.
[002] MALDI analysis is a useful tool for solving structural problems in biochemistry, immunology, genetics and biology. The samples are ionized on the gas platform and a time of flight analyzer (TOF) is used to measure the mass of the ions. TOF analysis begins when ions are formed and are accelerated to constant kinetic energy as they enter a region of fluctuation. They arrive at a detector after the flight hours that are provided to the square root of their masses. A mass spectrum is created because ions of different masses reach the detector at different times.
[003] Mass spectrometry can be a powerful tool in the areas of drug discovery and development, genotyping and proteome research. In addition, MALDI has already been used for the characterization and identification of bacteria and microorganisms. Current research trends are to analyze increasing sample numbers using individual sample amounts ranging from micromole levels to atomole levels. As a result, the samples are also getting smaller and smaller and there is a need for efficient and reliable acquisition of the correct amount of microorganisms and the accurate deposit of a sample of the quantity acquired on a target plate used in the MALDI instrument.
[004] In a typical MALDI TOF MS operation, the sample to be analyzed is marked or deposited on a metal plate (also called a target plate or MALDI plate), reagents are added (matrix) which support the ionization and then are dried to form crystals. In these instruments, the target plate is positioned in a fixed position on the MALDI instrument. The target plate has a plurality of deposition points (for example, 24 to 384 deposition points on a single target plate) and these deposition points have a fixed orientation with respect to the edges of the target plate. The target plate is positioned on an X-Y platform so that a sample obtained from a colony of microorganisms can be deposited at a selected deposition point. A high voltage potential is maintained between the target plate and a metal mesh. This tension can be maintained or pulsed, depending on the desired results and a vacuum is created in the chamber. A laser is fired at the sample / matrix and an ion cloud is formed. The voltage difference is used to accelerate the ions in a flight tube so that they can be analyzed. The analysis directly refers to the flight time for the mass of the ionized component.
[005] Various parameters can affect the quality of the results, including the flatness of the target, quantity and type of matrix, the concentration of the sample, the conductivity of the target sample, the accuracy of placement at the deposition point, as well as other variables.
[006] In particular, an important aspect is the treatment of the sample and the concentration of the sample. It is known that a suspension is made from a sample of a colony of microorganisms and that a researcher pipets a drop of the suspension obtained with the sample to a point of deposition of a target plate by hand. To provide a correct analysis, however, the suspension must contain sufficient sample concentration from the start.
[007] In the preparation of such a suspension of a sample of microorganisms, a portable device comprising a unit. The unit comprises a housing with a rotary drive motor contained therein, and a connector that is configured for releasably fixing the sample acquisition device to the unit. The sample acquisition device comprises a sample collection region, which is first placed in contact with the biological material (mainly grown on a culture plate) to be analyzed. Subsequently, the sample collection region is fixed to the rotating unit, placed in contact with a liquid medium contained in a tube and the rotating unit is activated for a certain period of time in such a way that a sample of biological material in the acquisition device of samples is released into the liquid medium. After the sample collection region is removed from the tube, the suspension tube maintains a suspension containing the sample, which, for example, can be used to perform MALDI analysis.
[008] However, the efficiency of the sample release from the sample acquisition region may, in some cases, be insufficient to perform a correct analysis of the microorganism suspended in the liquid medium. This can result in a prepared suspension that is not usable, resulting in a loss of time and money. In addition, since the sample collection region is rotated inside the tube, the tube must be of a sufficient size to allow rotation without the region of sample acquisition coming into contact with the inner wall of the tube, which can be harmful to the release of the microorganism in the liquid medium. Such a relatively large sized tube therefore contains a relatively large volume of liquid medium, which leads to a correspondingly long additional processing time of such liquid suspension medium. For example, the time that the liquid suspension containing the sample has to incubate in an incubator is sufficient for the amount of liquid in suspension. Therefore, there is a need for a method in which the preparation of a suspension of a sample of microorganisms is carried out automatically leading to a much more reproducible way for the preparation of such a suspension. In addition, there is a need for reliably and reproducibly releasing the sample at a target plate deposition point. SUMMARY OF THE INVENTION
[009] In order to solve at least one of the problems mentioned above, the present invention provides a method for locating and selecting a colony of microorganisms on a culture plate and identification of microorganisms in said colony selected with the use of MALDI, in which the said method comprises the automated steps of: locating and selecting a colony of microorganisms on a culture plate; obtain a sample of said selected microorganism colony; depositing at least some of said sample of said selected microorganism colony on a target plate; and transferring said target plate with said sample to an apparatus for performing MALDI for identification of said sample of said selected microorganism colony. It appears that the problems mentioned above mostly find their cause there because the steps are carried out manually and these steps are, therefore, subject to variation and undesirable errors, leading to incorrect results from the MALDI instrument , at additional costs and wasted time. By automating, each step of these problems can be overcome, at least to a large extent. In this field it was taken for granted that at least some of the steps can only be performed manually, however, in contrast here, the present invention provides the possibility, for the first time, to automate all the steps necessary to locate and select a colony of microorganisms and identify the microorganisms in said selected colony using MALDI.
[010] In an embodiment of a method according to the invention, the step of depositing at least some of said sample of said colony of microorganisms selected on a target plate is performed by direct placement of the sample obtained from said colony of microorganisms organisms selected on said target plate. When placing a sample directly on a target plate, it is not necessary to first prepare a suspension of said sample, thus circumventing any problems that may arise from preparing such a suspension. Preferably, the method comprises the additional automated step of overlaying a drop of a MALDI matrix solution on the portion of the sample deposited on the target plate.
[011] In an alternative modality of a process according to the invention, the step of depositing at least some of said sample of said colony of microorganisms selected on a target plate is carried out by the automated steps of: transferring said sample obtained for a suspension tube containing a quantity of a suspension medium; preparing a sample suspension, transferring at least a portion of said sample obtained in said suspension medium; obtaining a drop of said sample suspension; and transferring said drop of sample suspension onto said target plate. By fully automating, the preparation of a suspension the invention provides an accurate and reproducible method of using suspensions for the identification of microorganisms using MALDI. In particular, when the method further comprises the automated step of superimposing a drop of a MALDI matrix solution on said drop of sample suspension deposited on the target plate, the method of the invention is extremely suitable for the characterization of microorganisms. The best results of the analysis can be obtained in another embodiment of a method according to the invention, in which said drop of sample suspension deposited on the target plate is allowed to dry before said drop of MALDI matrix solution is superimposed. This alternative method of using a suspension is also extremely useful if another test or analysis is performed on the microorganism colony sample. Such additional analysis can, in a particularly efficient and reproducible manner, be carried out in an embodiment of a method according to the invention, wherein the method further comprises the automated steps of: obtaining a second drop of said sample suspension; depositing said second drop of sample suspension on a test culture plate; and transferring said test culture plate to an apparatus for carrying out a susceptibility test or other additional analysis. Consequently, the method of the invention can be used to automatically obtain or select a sample that can be fed into available ID / AST instruments including but not limited to BACTECTM, Phoenix, MGIT, BacT / Alert.
[012] The present invention also provides the possibility to carry out such additional analyzes in the event that no suspension is made from a sample obtained. In this situation, a particularly advantageous embodiment of a method according to the invention further comprises the automated steps of: obtaining a second sample of a colony of microorganisms from the culture plate; transferring said second sample from said selected microorganism colony; depositing at least some of said second sample of said colony of microorganisms on a selected test culture plate; and transferring said test culture plate to an apparatus for carrying out a susceptibility test or other additional test.
[013] Since in order to characterize and identify microorganisms, normally, a plurality of colonies are grown on a culture plate, it is still important that the sample be obtained from a colony of interest. If samples are taken from uninteresting colonies, efficient use of time and the MALDI instrument is compromised. According to the applicant's knowledge, until now there is no automated process or apparatus to discriminate between interesting and uninteresting colonies. However, the process of discriminating colonies can be at least partially automated while maintaining a very high certainty of correct discrimination in a modality of a method according to the invention, in which the method comprises, before the automated stage of locating and selecting a colony of microorganisms on a culture plate, the step of providing a culture plate comprising a number of colonies of microorganisms, obtaining an initial image of said culture plate, including all colonies of microorganisms, display of said initial image of said culture plate including all colonies of microorganisms on a monitor, and selection of at least one colony of microorganisms in said initial image. In this way a researcher or analyst can select colonies of interest based on education and complete knowledge. In a particular embodiment, said culture plate is provided with an individual identification identifying said culture plate, such as a bar code, and the method further comprises the step of storing said initial image of said culture plate including all the colonies, store information related to said at least one colony of microorganisms selected, store said identification of said culture plate in a central control computer's memory. In an additional modality, the researcher or analyst can manually insert the processing instructions in relation to the processing to which a colony of microorganisms selected from said culture plate must be subjected, said processing instructions being stored in said memory of said computer central control for later use.
[014] In another embodiment of the invention, the method comprises the automated steps of positioning said culture plate on a platform for a culture plate, obtaining an image of said culture plate positioned on said platform, obtaining the identification of said culture plate, compare the image obtained by the image device of said selection tool device with the initial stored image of said culture plate to obtain information on the location of the selected microorganism colony and, optionally, to obtain the processing instructions in relation to the processes to be carried out in said selected microorganism colony. By comparing the image of the culture plate when it is placed in the selection tool device with the initial image, the location of the selected colonies can be obtained automatically, for example, by comparing computerized images.
[015] In yet another embodiment of a method according to the invention, the method comprises the step of automatically preparing a suspension of a sample of microorganisms comprising the steps of: - providing a first selection tool and providing a positioning device with a selection tool holder to hold a selection tool, said positioning device being arranged for positioning a selection tool in a starting position above the location obtained from the selected microorganism colony in culture plate and to automatically lower and raise a selection tool towards, and away from, the culture plate and to position a selection tool in a transfer position, respectively; - positioning in said first selection tool in said positioning device selection tool holder; - by said positioning device, position the first selection tool in the starting position above the location obtained from the selected microorganism colony on the culture plate, automatically lower the first selection tool towards the culture plate in contact with the microorganism to selecting a sample of said microorganism, automatically raising the first selection tool with said sample of the microorganism away from the culture plate to the transfer position; - provide a suspension tube support to maintain a suspension tube; - position a suspension tube in the suspension tube support; - providing an automatic suspension medium dispenser to automatically dispense a suspension medium in a suspension tube maintained in the suspension tube holder; - through the automatic dispenser, automatically supply an initial amount of suspension medium to the suspension tube maintained in the suspension tube holder; - providing a transfer device to automatically transfer a selection tool from the transfer position of the positioning device to a position above a suspension tube maintained in the suspension tube holder, and to lower and raise a selection tool to inside and away from a suspension medium contained in a suspension tube, and to position a selection tool in a standby position above a suspension tube maintained in the suspension tube holder, respectively; - by said transfer device, automatically transfer said first selection tool with the sample of the microorganism from the transfer position of the positioning device to a position above the suspension tube maintained in the suspension tube holder, lowering said first selection tool with the microorganism sample for the suspension medium contained in the suspension tube; by the transfer device, oscillate the first selection tool in a vertical linear movement over a period of time, while the first selection tool with the sample of said microorganism is submerged in the suspension medium; and after said period of time has elapsed, lift the first selection tool away from the suspension medium contained in the suspension tube to the standby position; - providing a turbidity meter to measure the turbidity of a suspension medium contained in a suspension tube maintained in the suspension tube holder; - at least after the elapsed period of time during which the selection tool is oscillated, measure the turbidity of the suspension medium contained in the suspension tube maintained in the suspension tube support by the turbidity meter and provide a final measurement value indicative of the measured turbidity; - provide a controller communicatively connected to the positioning device, the transfer device, the automatic suspension medium dispenser and the turbidity meter to automatically control the movement of the positioning device, the movement of the transfer device, the operation of the automatic dispenser suspension medium and the operation of the turbidity meter, respectively; - by said controller: a) determine whether the final measured value is above a first threshold value previously stored in a memory of the controller, if so, perform step b); or if the final measured value is identical to or less than the first threshold value and identical to or greater than a second threshold value previously stored in the controller's memory, said first threshold value being equal to or greater than the second threshold value , if yes, perform step c); or if the final measured value is less than the second threshold value, if so, then perform step d); b) controlling the automatic suspension medium dispenser to supply an additional amount of suspension medium to the suspension tube; c) provide a signal that the suspension tube with the suspension can be removed from the suspension tube holder for further processing; or d) provide an additional selection tool; positioning said additional selection tool in said positioning device selection tool holder; by said positioning device, position the additional selection tool in the starting position above the culture plate, automatically lower the additional selection tool towards the culture plate in contact with the microorganism to select an additional sample of said microorganism , automatically lift the additional selection tool with said sample of the microorganism away from the culture plate to the transfer position; by said transfer device, automatically transfer said additional selection tool with the additional sample of the microorganism from the transfer position of the positioning device to a position above the suspension tube maintained in the transfer suspension tube holder, lowering said additional selection tool with the additional sample from the microorganism to the suspension medium contained in the suspension tube; by the transfer device, oscillate the additional selection tool in a vertical linear movement for a period of time while the additional selection tool with the additional sample of said microorganism is submerged in the suspension medium; and after said period of time has elapsed, lift the additional selection tool away from the suspension medium contained in the suspension tube to the standby position; at least, after the period of time during which the additional selection tool has been oscillated has elapsed, measure the turbidity of the suspension medium contained in the suspension tube held in the suspension tube holder by the turbidity meter and provide a measured value additional end indicative of the measured turbidity; and perform step a). In this way, it is possible to prepare a suspension of a sample of a microorganism in an advanced automatic way, while by means of the controller and the turbidity meter it is possible to supply a suspension tube containing a suspension medium, which contains a quantity of microorganism that is always sufficient (and reproducible) to carry out a correct analysis of the microorganism.
[016] In the form of a method for the automatic preparation of a suspension of a sample of microorganisms, according to the invention, the controller is arranged in such a way that the turbidity measurement step of the suspension medium contained in the tube of suspension maintained in the suspension pipe support by the turbidity meter is additionally carried out during the period of time during which the selection tool is oscillated, in which the turbidity meter is arranged to provide an online measurement value indicative of turbidity measured, during the time period during which the selection tool is oscillated for the controller. In this way, an extremely fast automatic determination of the amount of microorganisms in suspension can be obtained. In particular, if, during oscillation, the online measurement value of turbidity is equal to or less than the first threshold value and equal to or greater than the second threshold value, the controller controls the movement of the transfer device. such that the selection tool is raised to the standby position, and the controller further provides a signal that the suspension tube with the suspension can be removed from the suspension tube holder for further processing. In this way, the oscillation of the selection tool is interrupted when the suspension medium contains a sufficient amount of microorganisms, so that the method can be carried out with extremely efficient time.
[017] The mutual arrangement of the selection tool and the sensors of the turbidity meter is such that, during the oscillation of the selection tool, the selection tool does not obstruct the trajectory of the turbidity meter.
[018] In another modality of a method for the automatic preparation of a suspension of a sample of microorganisms, according to the invention, the controller is arranged to control the turbidity meter in which the measurement step of the turbidity of the suspension medium contained in the suspension tube maintained in the suspension tube support by the turbidity meter is started before the selection tool is submerged in the suspension medium contained in the suspension tube. In this way it is possible, for example, to check that the initial suspension medium used is not contaminated. In addition, this provides an indication of the starting value for turbidity which is useful in determining the final measured value.
[019] In yet another embodiment of a method for the automatic preparation of a suspension of a sample of microorganisms, according to the invention, the method further comprises the step of providing as a suspension tube support to maintain a tube suspension, a rotating suspension tube support for rotation of a suspension tube maintained in the rotating suspension tube support, the step of arranging the controller in such a way that it is communicatively connected with a rotating suspension tube support to control the rotation of the suspension tube support, and the step of arranging the controller in such a way that the suspension tube is rotated during the measurement of the turbidity of the suspension medium contained in the suspension tube. This rotation of the suspension tube allows turbidity measurements to be carried out in various positions within the suspension tube, which are spaced in rotation with each other leading to a more correct final measurement of the suspension's turbidity. Rotation, as such, is not necessary to release the sample from the selection tool, the vertical linear oscillating movement of the selection tool is more than enough to release the sample.
[020] Although the additional selection tool can be used which is different from the first selection tool, the method can be carried out economically, when in step d), the first selection tool is provided as a selection tool additional; and the positioning of said additional selection tool on said positioning device selection tool holder is carried out by the transfer device under the control of the controller.
[021] In yet another embodiment of a method for the automatic preparation of a suspension of a sample of microorganisms, according to the invention, the additional amount of suspension medium is determined by the controller based on the initial amount of medium suspension, the value of the final measure and the value of the first and / or second threshold value. This makes it possible to use only sufficient additional suspension medium if necessary and thus in this embodiment as little as possible of the suspension medium can be used.
[022] Since, according to the method of the invention for the automatic preparation of a suspension of a sample of microorganisms, the selection tool is oscillated with a vertical linear movement in relation to the suspension tube, the size of the suspension can be relatively small. This makes it possible that, in an embodiment of a method according to the invention, the controller can be arranged to control the automatic suspension medium dispenser such that the initial value provided is about 0.5 - 2 ml, preferably about 1 ml. Such a relatively small amount of suspension medium is sufficient to prepare a correct suspension of a sample of the microorganisms. In such a method for the automatic preparation of a suspension of a sample of microorganisms it is possible to use as a suspension tube, a suspension tube that has a substantially circular cross section with a diameter of about 6 to about 12 mm, from preferably about 10 mm, which is relatively small when compared to traditional tubes that have a diameter of about 16 mm. With such a relatively small suspension tube, a correct release of the sample from the selection tool is obtained when the controller is arranged to control the oscillation of the transfer device in such a way that the selection tool oscillates at a frequency between about 5 Hz to about 120 Hz, preferably from about 30 Hz to about 90 Hz, more preferably, about 50 Hz. Preferably, the controller is arranged to control the oscillation of the transfer device as so that the selection tool oscillates with an amplitude of about 0.5 mm to about 4 mm, preferably from about 2 mm to about 3 mm, which results in an optimal sample release from the tool of selection. In case the controller is willing to control the oscillation of the transfer device in such a way that the period of time during which the selection tool oscillates is from about 3 seconds to about 10 seconds, preferably about 6 seconds , it appears that, in almost all cases, the complete sample can be released from the selection tool.
[023] The relative values for frequency, amplitude and duration are dependent on the properties of the specific microorganism and, for example, its adherence to the selection tool. From the image inspection it can be deduced whether or not the sample was, at least, for the most part released from the selection tool, first using the aforementioned preferred values. If there is still any material left in the selection tool then the vertical oscillation is repeated within the ranges given at different values.
[024] Method for the automatic preparation of a suspension of a sample of microorganisms according to the invention can be obtained when the method additionally comprises the step of providing an automatic positioning and removal device from the culture plate to position and removing the culture plate comprising said microorganism on and from the platform, respectively. The controller is arranged in such a way that it is then communicatively connected to the automatic culture plate positioning and removal device to control the operation of the automatic culture plate positioning and removal device. In this positioning mode, a culture plate comprising said microorganism on the platform can be performed automatically under the control of the controller. Additional automation is carried out by providing an automatic suspension tube positioning and removal device to automatically position and remove a suspension tube on and from the suspension tube support, respectively. The controller is arranged in such a way that it is then connected communicatively to the automatic suspension tube positioning and removal device to control the operation of the automatic suspension tube positioning and removal device, so that the positioning of a suspension tube in the suspension tube holder can be made automatically under the control of the controller. Advantageously, the controller is arranged in such a way that a culture plate is allowed to be automatically removed from the platform by the positioning and automatic removal of the culture plate only after the signal that the suspension tube with the suspension can be removed from the suspension tube support for further processing is provided. In addition, it is therefore preferable that the controller is arranged in such a way that a suspension tube holder is automatically removed from the automatic suspension tube positioning and removal device only after the signal that the suspension tube with the suspension can be removed. removed from the suspension tube holder for further processing to be provided.
[025] In yet another embodiment of a method according to the invention, the method comprises the step of providing an identification mark on said suspension tube, and the step of storing the identification mark of said suspension tube, together with the properties of the suspension with a link to the identity of the culture plate from which the selected microorganism colony was obtained in the memory of said central control computer, the method can not only be operated automatically in an extremely efficient manner , but also the correct and fast processing of the obtained analysis results is improved.
[026] In yet another modality of a method according to the invention, in which the processing instructions for a selected microorganism colony indicate to directly place said sample obtained from said microorganism colony selected on said plate target, the method comprises the following automated steps: providing a first selection tool and providing a positioning device with a selection tool holder to hold a selection tool, said positioning device being arranged for positioning a selection in a starting position above the location obtained from the selected microorganism colony on the culture plate and to automatically lower and raise a selection tool towards and away from the culture plate and to position a selection tool in a transfer position, respectively; positioning said first selection tool in said positioning device selection tool holder; by said positioning device, position the first selection tool in the starting position above the location obtained from the selected microorganism colony on the culture plate, automatically lower the first selection tool towards the culture plate in contact with the microorganism to select a sample of said microorganism, automatically lift the first selection tool with said sample of the microorganism away from the culture plate to the transfer position; providing a support for the target plate to hold a target plate, said target plate having a plurality of deposition points; position a target plate on the target plate holder; provide a transfer device to automatically transfer the selection tool from the transfer position of the positioning device to a position above one of the deposition points of the target plate, and to lower the selection tool such that the sample of colony of microorganisms present in the selection tool comes into contact with the target plate, move the selection tool in a plane parallel to the plane of the target plate in such a way that the sample of colony of microorganisms is deposited on the deposition point, in particular covering at most approximately half of said one of the target plate deposition points; and lift said target plate selection tool. By depositing the sample automatically, a more reproducible and accurate deposition of the sample at the deposition point can then be achieved by manually depositing a sample. In addition, when the sample covers, at most approximately half of said one of the deposition points of a target plate, the results obtained from the analysis of the MALDI instrument on the part of the deposition point, which initially not covered with the sample are surprisingly extremely more accurate than the results obtained from the MALDI instrument analysis on the part of the deposition point, which was initially covered with the sample. Although the inventors do not intend to stick to a particular theory, it is believed that crystallization that occurs after a drop of matrix material is coated on the sample covering a part of the deposition point ensures that also the part of the deposition point at which not covered contains a quantity of sample material, and that this quantity is extremely adequate to provide excellent analysis results. In an alternative embodiment of a method according to the invention, in which in the case of processing instructions for a selected microorganism colony indicates obtaining a drop of said sample suspension and to transfer said drop onto said plate target, the method comprises the following automated steps: providing a pipetting tool in said selection tool device and providing a positioning device with a pipetting tool holder to maintain said pipetting tool, said positioning device being arranged to position said pipetting tool from a starting position above the suspension tube and to automatically lower and raise the pipetting tool into and away from the suspension and for positioning the pipetting tool from a transfer position, respectively; positioning said pipetting tool in said pipetting tool holder of the positioning device; by said positioning device, position the pipetting tool in the starting position above the suspension tube, lower the pipetting tool on the suspension in said suspension tube, operate the pipetting tool to select a quantity of suspension, raise the piping tool with said amount of suspension to the transfer position; said pipetting tool comprising a pressurizable chamber closed by a controlled valve containing the amount of suspension medium; providing a support for the target plate to hold a target plate, said target plate having a plurality of deposition points; position a target plate on the target plate holder; provide a transfer device to automatically transfer the pipetting tool from the transfer position of the positioning device to a position above one of the deposition points on the target plate, and to lower the pipetting tool to a predefined distance above the plate target, pressurize the chamber to a pressure in the range of about 0.5 bar to 1.1 bar, and open the valve for a time such that a drop of suspension with a volume in the range of about 0.5 to 3.0 ul is deposited at the point of deposition, in particular covering at most approximately half of said one of the points of deposition of the target plate; and lifting said pipetting tool from the target plate. Depending on the properties of the specific microorganism, for example, its stickiness, the pressure value and the opening time can be adjusted to obtain a small drop of suspension that can be prepared in a reproducible way and that, as result of the automated process, it can be accurately deposited on the target plate.
[027] In order to avoid cross contamination of a preferred embodiment of a method according to the invention, the shape of the pipetting tool, in particular the dispensing tip of the same, in such a way that the deposition of the drop of suspension on the target plate occurs in a free spreading mode. It was found that, depending on the type of micro-organism used, and especially on its stickiness, in addition to a choice of the correct pressure in the range mentioned above and the opening time of the valve in the range mentioned above, an adequate form of the Pipetting ensures that a drop of suspension can be deposited in a spread-free manner.
[028] In another embodiment of a method according to the invention, in which the method comprises the step of providing an identification mark on the target plate and, optionally, providing an identification mark at the deposition points of said target plate , and the step of storing the identification mark of said target plate and deposition points together with the properties of the suspension, with a link to the identity of the culture plate from which the selected microorganism colony was obtained in memory of said central control computer, the method can not only be operated automatically in an extremely efficient way, but also the correct and fast processing of the analysis results obtained is improved.
[029] The present invention also relates to a method for the automatic preparation of a suspension of a sample of microorganisms, said method comprises the steps of:
[030] - providing a platform for a culture plate comprising said microorganism;
[031] - position a culture plate comprising said microorganism on the platform;
[032] - provide a first selection tool and provide a positioning device with a selection tool holder to hold a selection tool, said positioning device being arranged for positioning a selection tool in a position starting above the culture plate and to automatically lower and raise a selection tool towards, and away from, the culture plate and to position a selection tool in a transfer position, respectively;
[033] - position said first selection tool on said support of the positioning device selection tool;
[034] - Using said positioning device, position the first selection tool in the starting position above the culture plate, automatically lower the first selection tool towards the culture plate in contact with the microorganism to select a sample from said microorganism, automatically lift the first selection tool with said sample of the microorganism away from the culture plate to the transfer position;
[035] - provide a suspension tube support to maintain a suspension tube;
[036] - position a suspension tube in the suspension tube support;
[037] - provide an automatic suspension medium dispenser to automatically dispense a suspension medium in a suspension tube maintained in the suspension tube holder;
[038] - by the automatic dispenser, automatically supply an initial amount of suspension medium to the suspension tube maintained in the suspension tube holder;
[039] - provide a transfer device to automatically transfer a selection tool from the transfer position of the positioning device to a position above a suspension tube maintained in the suspension tube holder, and to lower and raise a tool selection into and away from a suspension medium contained in a suspension tube, and to position a selection tool in a waiting position above a suspension tube held in the suspension tube holder, respectively;
[040] - by said transfer device, automatically transfer said first selection tool with the sample of the microorganism from the transfer position of the positioning device to a position above the suspension tube maintained in the suspension tube support , lower said first selection tool with the microorganism sample to the suspension medium contained in the suspension tube; by the transfer device, oscillate the first selection tool in a vertical linear movement for a period of time, while the first selection tool with the sample of said microorganism is submerged in the suspension medium; and after said period of time has elapsed, lift the first selection tool away from the suspension medium contained in the suspension tube to the standby position;
[041] - provide a turbidity meter to measure the turbidity of a suspension medium contained in a suspension tube maintained in the suspension tube holder;
[042] - at least, after the period of time during which the oscillating selection tool has elapsed, measure the turbidity of the suspension medium contained in the suspension tube maintained in the suspension tube support by the turbidity meter and provide a value final measurement indicative of the measured turbidity;
[043] - provide a controller communicatively connected to the positioning device, the transfer device, the automatic suspension medium dispenser and the turbidity meter to automatically control the movement of the positioning device, the movement of the transfer device, the operation the automatic suspension medium dispenser and the operation of the turbidity meter, respectively;
[044] - By said controller:
[045] a) determine if the final measured value is above a first threshold value previously stored in a controller memory, if yes, perform step b); or if the final measured value is identical to or less than the first threshold value and identical to or greater than a second threshold value previously stored in the controller's memory, said first threshold value being equal to or greater than the second threshold value , if yes, perform step c); or if the final measured value is less than the second threshold value, if so, then perform step d);
[046] b) control the automatic suspension medium dispenser to supply an additional amount of suspension medium to the suspension tube
[047] c) provide a signal that the suspension tube with the suspension can be removed from the suspension tube holder for further processing; or
[048] d) provide an additional selection tool; positioning said additional selection tool on said positioning device selection tool holder; by said positioning device, position the additional selection tool in the starting position above the culture plate, automatically lower the additional selection tool towards the culture plate in contact with the microorganism to select an additional sample of said microorganism , automatically lift the additional selection tool with said sample of the microorganism away from the culture plate to the transfer position; by said transfer device, automatically transfer said additional selection tool with the additional sample of the microorganism from the transfer position of the positioning device to a position above the suspension tube maintained in the transfer suspension tube holder, lowering said additional selection tool with the additional sample from the microorganism to the suspension medium contained in the suspension tube; by the transfer device, oscillate the additional selection tool in a vertical linear movement for a period of time while the additional selection tool with the additional sample of said microorganism is submerged in the suspension medium; and after said period of time has elapsed, lift the additional selection tool away from the suspension medium contained in the suspension tube to the standby position; at least, after the period of time during which the additional selection tool has been oscillated has elapsed, measure the turbidity of the suspension medium contained in the suspension tube held in the suspension tube holder by the turbidity meter and provide a measured value additional end indicative of the measured turbidity; and perform step a). In this way, it is possible to prepare a suspension of a sample of a microorganism in an advanced automatic way, while by means of the controller and the turbidity meter it is possible to supply a suspension tube containing a suspension medium, which contains a quantity of microorganism that is always sufficient (and reproducible) to carry out a correct analysis of the microorganism.
[049] In the form of a method for the automatic preparation of a suspension of a sample of microorganisms, according to the invention, the controller is arranged in such a way that the turbidity measurement step of the suspension medium contained in the tube of suspension maintained in the suspension pipe support by the turbidity meter is additionally carried out during the period of time during which the selection tool is oscillated, in which the turbidity meter is arranged to provide an online measurement value indicative of turbidity measured, during the time period during which the selection tool is oscillated for the controller. In this way, an extremely fast automatic determination of the amount of microorganisms in suspension can be obtained. In particular, if, during oscillation, the online measurement value of turbidity is equal to or less than the first threshold value and equal to or greater than the second threshold value, the controller controls the movement of the transfer such that the selection tool is raised to the standby position, and the controller further provides a signal that the suspension tube with the suspension can be removed from the suspension tube holder for further processing.
[050] In another embodiment of a method for the automatic preparation of a suspension of a sample of microorganisms, according to the invention, the controller is arranged to control the turbidity meter in which the measurement step of the turbidity of the suspension medium contained in the suspension tube maintained in the suspension tube support by the turbidity meter is started before the selection tool is submerged in the suspension medium contained in the suspension tube. In this way it is possible, for example, to check that the initial suspension medium used is not contaminated. This can, for example, be done by taking a measurement of the turbidity of a pipe suspension and comparing the value obtained with a predetermined value indicating that the suspension is not contaminated. In case the difference exceeds a threshold value, then, for example, a warning signal can be given indicating that the suspension is contaminated.
[051] In yet another embodiment of a method for the automatic preparation of a suspension of a sample of microorganisms, according to the invention, the method further comprises the step of providing as a suspension tube support to hold a tube suspension, a rotating suspension tube support for rotation of a suspension tube maintained in the rotating suspension tube support, the step of arranging the controller in such a way that it is communicatively connected with a rotating suspension tube support to control the rotation of the suspension tube support, and the step of arranging the controller in such a way that the suspension tube is rotated during the measurement of the turbidity of the suspension medium contained in the suspension tube. This rotation of the suspension tube allows turbidity measurements to be carried out in various positions within the suspension tube, which are spaced apart in rotation which leads to a more correct final measurement of the suspension turbidity. Rotation, as such, is not necessary to release the sample from the selection tool, the vertical linear oscillating movement of the selection tool is more than enough to release the sample.
[052] Although the additional selection tool can be used which is different from the first selection tool, the method can be carried out economically, when in step d), the first selection tool is provided as a selection tool additional; and the positioning of said additional selection tool on said positioning device selection tool holder is carried out by the transfer device under the control of the controller.
[053] In yet another embodiment of a method for the automatic preparation of a suspension of a sample of microorganisms, according to the invention, the additional amount of suspension medium is determined by the controller based on the initial amount of medium suspension, the value of the final measure and the value of the first and / or second threshold value. This makes it possible to use only sufficient additional suspension medium if necessary and thus in this embodiment as little as possible of the suspension medium can be used. In case the measured value indicates that the amount of microorganisms is very low, then the control system controls the selection instrument to obtain an additional sample from the same colony selected, and the process is repeated.
[054] Since according to the method of the invention for the automatic preparation of a suspension of a sample of the microorganisms the selection tool is oscillated with a vertical linear movement, only the size of the suspension tube can be relatively small. This makes it possible that, in an embodiment of a method according to the invention, the controller can be arranged to control the automatic suspension medium dispenser such that the initial amount supplied is about 0.5 - 2 ml, preferably about 1 ml. Such a relatively small amount of a suspension medium is sufficient to prepare a correct suspension of a sample of the microorganisms. In such a method for the automatic preparation of a suspension of a sample of microorganisms it is possible to use as a suspension tube, a suspension tube that has a substantially circular cross section with a diameter of about 6 to about 12 mm, from preferably about 10 mm, which is relatively small when compared to traditional tubes that have a diameter of about 16 mm. With these relatively small suspension tubes, the correct release of the sample from the selection tool is achieved when the controller is arranged to control the oscillation of the transfer device in such a way that the selection tool oscillates at a frequency between about 5 Hz to about 120 Hz, preferably from about 30 Hz to about 90 Hz, more preferably, about 50 Hz. Preferably, the controller is arranged to control the oscillation of the transfer device in such a way that the selection tool oscillates with an amplitude of about 0.5 mm to about 4 mm, preferably from about 2 mm to about 3 mm, which results in an optimal sample release from the tool of selection. In case the controller is willing to control the oscillation of the transfer device in such a way that the period of time during which the selection tool oscillates is from about 3 seconds to about 10 seconds, preferably about 6 seconds , it appears that, in almost all cases, the complete sample can be released from the selection tool.
[055] The invention also relates to an apparatus for the automatic preparation of a suspension of a sample of microorganisms for carrying out a method for locating and selecting a colony of microorganisms on a culture plate and identification of microorganisms in said selected colony with the use of MALDI or for carrying out a step of a method for the automatic preparation of a suspension of a sample of microorganisms, according to the methods of the invention, said apparatus comprising:
[056] - a platform for a culture plate comprising said microorganism;
[057] - A first tool and an additional selection tool and a positioning device with a selection tool holder to hold a selection tool, said positioning device being arranged for positioning a selection tool in one position starting above the culture plate and to automatically lower and raise a selection tool towards, and away from, the culture plate and to position a selection tool in a transfer position, respectively;
[058] - a suspension tube support for maintaining a suspension tube;
[059] - an automatic suspension medium dispenser to automatically dispense a suspension medium in a suspension tube maintained in the suspension tube holder;
[060] - a transfer device for automatically transferring a selection tool from the transfer position of the positioning device to a position above a suspension tube maintained in the suspension tube holder, and for lowering and raising a selection into and away from a suspension medium contained in a suspension tube, and to position a selection tool in a waiting position above a suspension tube maintained in the suspension tube holder, respectively, said device being still arranged to oscillate a selection tool in a vertical linear movement during a transfer time period;
[061] - a turbidity meter to measure the turbidity of a suspension medium contained in a suspension tube maintained in the suspension tube holder and to provide a final measurement value indicative of the measured turbidity;
[062] - a controller communicatively connected to the positioning device, the transfer device, the automatic suspension medium dispenser and the turbidity meter to automatically control the movement of the positioning device, the movement of the transfer device, the operation of the automatic suspension dispenser and half-turbidity meter operation, respectively;
[063] - Said controller being willing to:
[064] a) determine whether the final measured value is above a first threshold value previously stored in a controller memory, if so, said controller being arranged to perform step b); or if the final measured value is identical to or less than the first threshold value and identical to or greater than a second threshold value previously stored in the controller's memory, said first threshold value being equal to or greater than the second threshold value , if so, said controller being willing to perform step c); or if the final measured value is less than the second threshold value, if so, said controller being arranged to perform step d);
[065] b) controlling the automatic suspension medium dispenser to supply an additional amount of suspension medium to the suspension tube;
[066] c) provide a signal that the suspension tube with the suspension can be removed from the suspension tube holder for further processing; or
[067] d) position said additional selection tool on said positioning device selection tool holder; by said positioning device, position the additional selection tool in the starting position above the culture plate, automatically lower the additional selection tool towards the culture plate in contact with the micro-organism to select an additional sample from said micro -organism, automatically lift the additional selection tool with said microorganism sample away from the culture plate to the transfer position; by said transfer device, automatically transfer said additional selection tool with the additional sample of the microorganism from the transfer position of the positioning device to a position above the suspension tube maintained in the transfer suspension tube holder, lowering said additional selection tool with the additional sample from the microorganism to the suspension medium contained in the suspension tube; by the transfer device, oscillate the additional selection tool in a vertical linear movement for a period of time while the additional selection tool with the additional sample of said microorganism is submerged in the suspension medium; and after said period of time has elapsed, lift the additional selection tool away from the suspension medium contained in the suspension tube to the standby position; at least, after the period of time during which the additional selection tool has been oscillated has elapsed, measure the turbidity of the suspension medium contained in the suspension tube held in the suspension tube holder by the turbidity meter and provide a measured value additional end indicative of the measured turbidity; and perform step a).
[068] In another embodiment of an apparatus according to the invention, the controller is arranged to control the turbidity meter such that the measurement of the turbidity of the suspension medium contained in the suspension tube held in the suspension tube holder by the meter of turbidity is started before the selection tool is submerged in the suspension medium contained in the suspension tube.
[069] In yet another embodiment of an apparatus according to the invention, the suspension tube support for holding a suspension tube is a rotating suspension tube support for rotating a suspension tube maintained in the suspension tube support rotary, in which the controller is arranged to be communicatively connected with the rotating suspension tube holder to control the rotation of the suspension support tube, and said controller is arranged to rotate the suspension tube during the turbidity measurement of the suspension medium contained in the suspension tube.
[070] In an advantageous embodiment of an apparatus according to the invention, in step d), the first selection tool is provided as an additional selection tool; and said controller is arranged to control the transfer device to position said additional selection tool on said positioning device selection tool holder.
[071] Preferably, the controller is willing to determine the additional amount of suspension medium, based on the initial amount of suspension medium, the final measurement value and the value of the first and / or second threshold value. In particular, the controller is arranged to control the automatic suspension medium dispenser such that the initial value provided is about 0.5 - 2 ml, preferably about 1 ml. In another embodiment of an apparatus according to the invention, the controller is arranged to control the oscillation of the transfer device in such a way that the selection tool oscillates at a frequency between about 5 Hz to about 120 Hz, preferably , from about 30 Hz to about 90 Hz, more preferably from about 50 Hz. In addition, the controller can be arranged to control the oscillation of the transfer device in such a way that the selection tool oscillates with an amplitude from about 0.5 mm to about 4 mm, preferably from about 2 mm to about 3 mm, and to control the oscillation of the transfer device in such a way that the period of time during which the selection oscillates is about 3 seconds to about 10 seconds, preferably about 6 seconds.
[072] In an inventive device for the automatic preparation of a suspension of a sample of microorganisms, the suspension tube can have a substantially circular cross section with a diameter of about 6 to about 12 mm, preferably from about 10 mm.
[073] A fully automatic device according to the invention, when the apparatus comprises an automatic positioning and removal device of the culture plate to automatically position and remove the culture plate comprising said microorganism and from the platform, respectively said controller being arranged to be communicatively connected with the automatic culture plate positioning and removal device to control the operation of the automatic culture plate positioning and removal device, and to automatically position a culture plate comprising said microorganism on the platform, and when the apparatus comprises an automatic suspension tube positioning and removal device to automatically remove and position a suspension tube on and from the suspension tube support, respectively, said controller being arranged to connect communicatively with the automatic device suspension tube positioning and removal to control the operation of the automatic suspension tube positioning and removal device, and to automatically position a suspension tube in the suspension tube holder. In this case, it is therefore preferable that the controller is prepared to allow a culture plate to be automatically removed from the platform by the positioning and automatic culture plate removal device only after the signal that the suspension tube with the suspension can be removed. from the suspension tube holder for further processing be provided. In addition, the controller is then preferably arranged to automatically remove a suspension tube holder from the automatic suspension positioning and removal tube device only after the signal that the suspension tube with the suspension can be removed suspension tube support for further processing is provided.
[074] The invention also relates to a method for automatically depositing a sample of a colony of microorganisms on a deposition point of a target MALDI plate, in which the method comprises the step of depositing the colony sample of microorganisms on the deposition point so that the sample covers at most approximately half of said one of the deposition points on the target plate. It was discovered that, then, when the sample covers, at most approximately half of said one of the points of deposition of a target plate, the results obtained from the analysis of MALDI instrument of the part of the point of deposition that was not initially covered with the sample are surprisingly much more accurate than the results obtained from the MALDI instrument analysis on the part of the deposition point, which was initially covered with the sample. It is assumed that the crystallization that occurs after a drop of matrix material has been superimposed on the sample covering part of the deposition point also ensures that the part of the deposition point, which has not been covered, contains a quantity of sample material, and that this amount is extremely adequate to provide excellent analysis results. The physical or chemical processes that are the cause for this effect at the moment are not clear, but perhaps more clarity can arise when the underlying fundamental processes of MALDI are known. In one embodiment of such a method, the sample of a colony of microorganisms is in the form of a suspension, in which the sample of the suspension is deposited at a point of deposition of the target plate in the form of a drop with a volume of a range of about 0.5 to 3.0 µl.
[075] The invention also relates to a method for automatically depositing a drop of a suspension containing a sample of a colony of microorganisms on a deposition point of a target MALDI plate, in which the method comprises the following steps automated: - providing a pipetting tool from a selection tool device and providing a positioning device with a pipetting tool holder to hold said pipetting tool, said positioning device being arranged to position said pipetting tool in an initial position above a tube, maintaining said suspension containing a sample of a colony of microorganisms, and to automatically lower and raise the pipetting tool into and out of the suspension and for positioning the pipetting tool in a transfer position, respectively; - positioning said pipetting tool in said support of the pipetting tool of said positioning device; - by said positioning device, position the pipetting tool in the starting position above the suspension tube, lower the pipetting tool in the suspension in said suspension tube, operating the pipetting tool to select a quantity of suspension, raise the piercing tool with said amount of suspension to the transfer position; said pipetting tool comprising a pressurizable chamber closed by a controlled valve containing the amount of suspension medium; - providing a target plate support for holding the target plate, said target plate having a plurality of deposition points; - position the target plate on the target plate support; - provide a transfer device to automatically transfer the pipetting tool from the transfer position of the positioning device to a position above one of the deposition points of the target plate, and to lower the selection tool at a predefined distance above the target plate, pressurize the chamber to a pressure in the range of about 0.5 bar to 1.1 bar, and open the valve for a time such that a drop of suspension with a volume in the range of about 0.5 3.0 ul is deposited on said one of the deposition points; and - lifting from said pipetting tool from the target plate. Preferably, the shape of the pipetting tool is such that the deposition of the drop of the suspension on the target plate occurs free of liquid spillage. In particular, the method comprises pressurizing the chamber to such a pressure in the range of about 0.5 bar to 1.1 bar, and opening the valve for a time such that a drop of suspension with a volume of about a range 0.5 to 3.0 ul is deposited in a maximum of half of one of the deposition points. It was discovered that, then, when the sample covers, at most approximately half of said one of the points of deposition of a target plate, the results obtained from the analysis of MALDI instrument from the point of deposition, which initially not covered with the sample are surprisingly much more accurate than the results obtained from the MALDI instrument analysis on the part of the deposition point, which was initially covered with the sample. It is assumed that the crystallization that occurs after a drop of matrix material is superimposed on the sample covering a part of the deposition point also ensures that the part of the deposition point, which was not covered, contains an amount of sample material, and that this quantity is extremely adequate to provide excellent results of the analysis. The physical or chemical processes that are the cause for this effect at the moment are not clear, but perhaps more clarity can arise when the underlying fundamental processes of MALDI are known. BRIEF DESCRIPTION OF THE DRAWINGS
[076] The invention will be further explained with reference to Figure 1, in which an exemplary, non-limiting embodiment of an apparatus of the invention is shown, in which Figure 1, will also be used to explain exemplary embodiments of the method according to the invention. . DETAILED DESCRIPTION OF EXEMPLARY MODALITIES
[077] In the present invention MALDI or MALDI-TOF-MS is used to identify microorganisms. In a MALDI TOF MS operation, a sample of a colony of microorganisms is marked or deposited on a target plate, which is held in a fixed position on the MALDI instrument. Such a target plate has a plurality of deposition points (for example, deposition points 24 to 384 on a single target plate) and these deposition points have a fixed orientation with respect to the edges of the target plate. The target plate is positioned on an XY platform so that a sample obtained from a colony of microorganisms can be deposited at a selected deposition point, where the location where a specific sample was deposited is indicated by the XY parameters and is stored in a central control computer's memory.
[078] Although not shown in detail in Figure 1, the target plate is positioned below a transfer lane 18 in a position indicated by B. A sample can be transferred along transfer lane 18, from a transfer plate. culture 3 and / or a suspension tube 11 on top of the target plate in position B, where the sample is lowered until it is deposited at a deposition point on the target plate.
[079] Although the invention is described in detail below with reference to the preparation of a suspension containing a sample and the deposition of a drop of said suspension, at a point of deposition of a target plate, the present invention also relates to the deposition directly of a sample obtained (selected) from a culture plate at a target plate deposition point.
[080] In general, in this last method, a colony of microorganisms is detected and located automatically on a culture dish. A sample of the said colony of selected microorganisms is obtained automatically, for example, by a selection tool, which is placed in contact with the colony. At least some of the said sample of said selected microorganism colony is directly deposited on a target plate automatically, by lowering the selection tool in such a way that the sample of microorganism colony present in the tool of selection, contact the target plate, and moving the selection tool in a plane parallel to the plane of the target plate in such a way that the colony sample of microorganisms is deposited on the deposition point, in particular covering at most approximately half of said one of the points of deposition of the target plate. After that, the target plate with said sample is automatically transferred to an MALDI device for identification of said sample of said selected microorganism colony.
[081] More detailed, this method comprises the following automated steps: - providing a first selection tool and providing a positioning device with a selection tool holder to maintain a selection tool, said positioning device being arranged for the positioning a selection tool in a starting position above the location obtained from the selected microorganism colony on the culture plate and to automatically lower and raise a selection tool towards, and away from, the culture plate and to position a selection tool in a transfer position, respectively; - positioning said first selection tool on said support of the positioning device selection tool; - by said positioning device, position the first selection tool in the starting position above the location obtained from the selected microorganism colony on the culture plate, automatically lower the first selection tool towards the culture plate in contact with the microorganism to selecting a sample of said microorganism, automatically lifting the first selection tool with said sample of the microorganism away from the culture plate to the transfer position; - providing a support for the target plate to hold a target plate, said target plate having a plurality of deposition points; - position a target plate on the target plate support; - provide a transfer device to automatically transfer the selection tool from the transfer position of the positioning device to a position above one of the target plate deposition points, and to lower the selection tool in such a way that the sample colony of microorganisms present in the selection tool contact the target plate, move the selection tool in a plane parallel to the plane of the target plate in such a way that the sample of colony of microorganisms is deposited on the point deposition, in particular covering at most approximately half of said one of the deposition points of the target plate; and lift said target plate selection tool. A drop of a MALDI matrix solution is then automatically placed in the amount of the sample deposited on the target plate.
[082] In the event that the same colony of microorganisms is subjected to analysis or another test, such as, but not limited to, an Antibiotic Susceptibility Test (AST) a second sample of said selected microorganism colony can be similarly obtained to obtain the initial sample. Once the location of the colony of microorganisms in the culture plates has already been selected, and is therefore "known" to the central control computer, it is possible to easily and reproducibly obtain a second sample from the same colony. At least some of the second sample of said selected microorganism colony is then transferred and deposited on a test culture plate, which can, for example, be automatically placed in the other position below transfer lane 18. Subsequently, said test culture plate is automatically transferred to a device for a susceptibility test or other additional analysis.
[083] Since to perform the characterization and identification of microorganisms, normally, a plurality of colonies are grown on a culture plate, and in addition to a plurality of different culture plates being used, the invention provides the possibility of identifying each culture plate separately, for example, by means of a bar code and, in addition, each colony of interest in a single culture plate is selected and given with an identification mark. To this end, prior to the automated step of locating and selecting a colony of microorganisms on a culture plate, the method according to the invention comprises a step of providing a culture plate comprising a number of micro colonies -organisms, obtaining an initial image of said culture plate, including all colonies of microorganisms, displaying said initial image of said culture plate, including all colonies of microorganisms on a monitor, and selection of at least one colony of microorganisms in said initial image. In this way, a researcher or analyst can select the colonies of interest based on education and deep knowledge. Since each culture plate is provided with an individual identification identifying said culture plate, such as a bar code, the initial image of said culture plate, including all colonies is stored, and information related to said, at least one colony of selected microorganisms is stored (preferably with links indicated in the (electronic) initial image), storing all said information and identification of the culture plate in a central control computer's memory allows for a much more correct processing . In this way the only manual operation is the act of selecting interesting colonies, while all relevant data is processed in an automated way. Optionally, the researcher or analyst can manually insert the processing instructions in relation to the treatment to which a colony of microorganisms selected from said culture plate must be subjected, said processing instructions being also stored in said memory of said control computer. central for later use. After this manual act, all other steps are carried out fully automated in a reliable and efficient manner. For this additional automated processing, the culture plate is automatically positioned on a platform for a culture plate of a selection tool device that comprises an image forming device. An image of said culture plate positioned on said selection tool device is obtained, and together with the identification of said culture plate, it is possible to compare the image obtained by the image device of said selection tool device with the image initial stored of said culture plate and, thus, information about the location of the selected microorganism colony and, optionally, about the processing instructions in relation to the processes to be carried out in said selected microorganism colony. By comparing the image of the culture plate when it is placed on the selection tool device with the initial image, the location of the selected colonies can be obtained automatically, for example by comparing the computerized images. In addition, each target plate is provided with an identification mark and, optionally, each deposition point of said target plate has an identification mark or individual local identifier, so that after storing the identification mark of said target plate and deposition points together with the properties of the suspension with a link to the identity of the culture plate from which the selected microorganism colony was obtained in the memory of said central control computer a correct connection of the results obtained for MALDI to the colony The specificity of the microorganism to be tested is possible in a correct and automated way.
[084] Now, an embodiment of a method of the invention will be described, in which a suspension is made from a sample of a colony of microorganisms selected from a culture plate in conjunction with a modality of an apparatus for perform such a method.
[085] Figure 1 schematically shows an embodiment of an apparatus 1 for the automatic preparation of a sample suspension of a microorganism according to the invention. Said apparatus 1 comprises a platform 2 for a culture plate 3 comprising a microorganism 4 on a nutritive layer 5, such as an agar gel layer.
[086] The device 1 also comprises a first selection tool 6 and an additional selection tool 7. A positioning device 8 comprises a selection tool holder 9 so that, in the shown mode, it can maintain a selection tool releasably, in modality shown in Figure 1, the selection tool holder 9 maintains the first selection tool 6. The positioning device 8 is arranged to position the first selection tool 6 in a starting position (shown in solid lines in Figure 1) above the culture plate 3 and is arranged to lower and raise the first automatic selection tool 6 towards, and away from, the culture plate 3, such that the first selection tool 6 can be positioned in one position (indicated by dashed lines 6 '), in which it comes into contact with microorganism 4 and selects a sample of said microorganism. After the first selection tool 6 selects a sample, the positioning device 8 lifts and positions the first selection tool 6 in a transfer position, which in the modality shown in Figure 1 is identical to the starting position. In other embodiments, the starting and transfer points may be different from each other.
[087] Apparatus 1 according to the invention further comprises a suspension tube holder 10 for holding a suspension tube 11, which can contain a suspension medium which is dispensed from an automatic suspension medium dispenser 12, which in the embodiment shown has a dispensing nozzle 13 to automatically dispense a suspension medium 14 in the suspension tube 11 maintained in the suspension tube support 10. In the present embodiment, the suspension support tube 10 is a tube support rotating suspension rod to rotate the suspension tube 11 about a vertical axis, A.
[088] A transfer device 15 is incorporated into the apparatus of the invention 1 to automatically transfer a selection tool from the transfer position of the positioning device 8 to a position above the suspension tube 11 held in the suspension tube holder 10 In the embodiment shown, the transfer device 15 comprises a transfer support 16 with gripping means 17 for releasably securing a selection tool. The transfer device 15 can in a manner known per se, be mounted on a transfer track 18, such as a rail, for linear movement therein, as indicated by the arrows. In this way, the transfer device 15 can be moved to the positioning device 8, such that the gripping means 17 can reach the selection tool from the positioning device 8, the support of the selection tool 9 thereof. releasing the selection tool after the gripping means 17 have grasped the selection tool. In the modality shown in Figure 1, the second tool or additional selection tool 7 having previously captured a sample 19 of the microorganism 4 is positioned over the suspension tube 11, by the transfer device 15 in a starting position indicated by the lines solid. The transfer device 15 is arranged to lower the second selection tool 7 into the suspension medium 14 contained in the suspension tube 11, in which the position of the second selection tool 7 'with the sample 19 is submerged in the suspension medium 14 , as indicated by dashed lines in Figure 1. In this position, the transfer device 15 is activated to oscillate the second selection tool 7 in a vertical linear movement for a period of time that is sufficient for the sample 19 to be released from the second selection tool 7. Subsequently, the transfer device 15 positions the second selection tool 7 in a waiting position, above the suspension tube 11, which is the waiting position in the mode shown in Figure 1 identical to the initial position . In other modalities of the waiting position, as well as the starting position may be different from each other.
[089] The apparatus of the invention is further provided with a turbidity meter 20 to carry out the turbidity measurements of the suspension medium 14 contained in the suspension tube 11 maintained in the suspension tube holder 10. As generally known in the art a meter turbidity can provide measurement values that are a measure of the concentration of the material, in this case, the concentration of a microorganism in suspension in the suspension medium. In the embodiment shown in Figure 1, the turbidity meter 20 comprises a laser 21, which transmits the laser light to and through the suspension medium and a sensor 22 that detects the amount of laser light transmitted through the suspension medium. In addition, there is an additional sensor (not shown in the drawing), which is for example arranged perpendicularly to the laser light path to detect the amount of laser light that has been dispersed by the suspension.
[090] The operation of the device of the invention is controlled by a controller 23, for example, which comprises a microprocessor, which is connected communicatively (as indicated by the signal lines) to the positioning device 8, the transfer device 15, dispenser of the automatic suspension medium 12, and turbidity meter 20 to automatically control the movement of the positioning device 8, the movement of the transfer device 15, the operation of the automatic suspension medium dispenser 12 and the operation of the turbidity meter 20, respectively. In addition, controller 23 can be directly connected communicatively to other parts of the device, such as, for example, the selection tool holder 9, the transfer holder 16, the laser 21 and the sensor 22.
[091] In the mode shown in Figure 1, controller 23 is configured to control the turbidity meter 20 in such a way that the measurement of the turbidity of the suspension medium 14 is started before the second selection tool 7 is submerged in the suspension medium 14. Additionally, controller 23 controls the rotating suspension tube support 10 to initiate rotation of the suspension tube 11 held in support 10, before the second selection tool 7 is submerged in the suspension medium 14, and to maintain rotation of the tube 11, during the turbidity measurement of the suspension medium 14. In the mode shown, the controller 23 controls the turbidity meter 20 in such a way that the turbidity measurement is carried out during the total period of time during which the second selection tool 7 is oscillated. In this way, the turbidity meter 20 provides an online measurement value with the controller 23, which is indicative of the measured turbidity value and, therefore, of the microorganism concentration, during the period of time during which the second selection tool is oscillated.
[092] Controller 23 comprises a memory in which a first and a second threshold value are stored, wherein said first threshold value is equal to or greater than the second threshold value. If the turbidity measurement value provided by the turbidity meter is equal to or between the first and the second threshold value, the concentration / amount of microorganisms in the suspension medium is sufficient to allow the suspension tube with the suspension to be processed. In that case, controller 23 provides a signal that the suspension tube can be treated later. In addition, in this situation the second selection tool 7 can be eliminated, for example by transferring the transfer device to a position in which C means the grip 17 are activated to release the second selection tool 7.
[093] If the final measured value of the turbidity meter is above the first threshold value previously stored in a memory of controller 23, then the concentration of the microorganism is too high to allow the suspension tube to be processed. In this situation, controller 23 controls the automatic suspension of dispensing medium 12 to supply an additional amount of suspension medium to the suspension tube 11. This additional amount of suspension medium is based on the initial amount of suspension medium, the value final measurement and at the value of the first and / or second threshold value so that the addition of an additional amount of suspension medium to the suspension medium already present in the suspension of tube 11 will lead to a concentration of microorganisms in the suspension medium, which satisfies the requirement for further processing, as can be confirmed by an additional measurement or turbidity by the turbidity meter of 20.
[094] If the final measured value of the turbidity meter below 20 is the second threshold value, which means that the concentration of microorganisms in the suspension medium is too low, controller 23 controls the device 1 so that an additional sample of microorganism is captured by the first selection tool 6 (alternatively, the second or an additional selection tool can be used to select an additional sample). Thus, the controller 23 in this case controls the transfer positioning device 15 such that the second selection tool 7 is discarded as described above. Then (or simultaneously), the first selection tool 6 in the selection tool holder 9 of the positioning device 8 is reduced from the start position above the culture plate 3 towards the culture plate and in contact with microorganism 4 to take an additional sample of said microorganism. After that, the first selection tool 6 is automatically lifted with the additional sample of the microorganism away from the culture plate to the transfer position. Then, said transfer device automatically transfers the first selection tool with the additional sample of the microorganism from the transfer position of the positioning device 8 to a position above the suspension tube 11 The first selection tool 6 with the additional sample of the microorganism, the suspension of product 14 is lowered and is oscillated by the transfer device 15, in a vertical linear movement for a period of time to release an additional sample of said microorganism in the medium of suspension. Again, turbidity is measured during oscillation, and the measured value is compared with the first and second threshold values stored in the controller 23 memory. In this case, controller 23 can be arranged to control the movement of the transfer device. 15, such that the first selection tool 6 is raised to the standby position during oscillation, if the value on the turbidity measurement line performed by the turbidity meter 20 is equal to or less than the first threshold value and equal to or greater than the second threshold value.
[095] The suspension tubes, which are particularly useful for the apparatus of the invention have a substantially circular cross section with a diameter of about 6 to about 12 mm, preferably about 10 mm. In these relatively small suspension tubes the controller 23 can control the automatic suspension dispense medium 12, so that the initial value provided of suspension medium is about 0.5 - 2 ml, preferably about 1 ml.
[096] The oscillation of the transfer device 15 is controlled by the controller 23, such that the selection tool oscillates at a frequency between about 5 Hz to about 120 Hz, preferably from about 30 Hz to about 90 Hz, more preferably, from about 50 Hz, with an amplitude of about 0.5 mm to about 4 mm, preferably from about 2 mm to about 3 mm. The controller is furthermore arranged to control the oscillation of the transfer device 15 such that the period of time during which the selection tool oscillates is from about 3 seconds to about 10 seconds, preferably from about 6 seconds.
[097] The apparatus of the invention 1, furthermore comprises a conveyor 24 from which the final position can form the platform 2 for the culture plate or as shown in Figure 1 a conveyor 24 and a platform 2, which are mutually positioned in such a way so that a culture plate can be transported to the stage and removed from the appropriate operating platform of the conveyor 24. The conveyor 24 is controlled by the controller 23 for the positioning and removal of a culture plate comprising said microorganism on, and from, the platform, respectively automatically. Note that different modes for positioning and removing a culture plate in and from the platform automatically, respectively, have not been shown, respectively, can be used. In particular, controller 23 is configured to allow a culture plate to be automatically removed from the platform by the automatic culture plate positioning and removal device only after the signal that the suspension tube with the suspension can be removed from the holder suspension tube for further processing has been provided. This ensures that it is always possible to take an additional sample, if necessary.
[098] As shown in Figure 1, the apparatus of the invention 1 further comprises an automatic suspension positioning tube and removing device 25 for positioning and removing a suspension tube and suspension tube support, respectively, automatically. When positioning the automatic suspension tube shown, the removal of the device 25 comprises gripping means 26 for releasably securing a suspension tube 11 '. Again, controller 23 is configured to be communicatively connected for automatic suspension tube positioning and removal device 25, 26 to control the operation of the automatic suspension tube positioning and removal device, and to automatically position a suspension tube in the suspension tube holder. Controller 23, in particular, is arranged to automatically remove a suspension tube holder from the automatic positioning and removal device of the 25, 26 suspension tube, only after the sign that the suspension tube with the suspension can be removed from the support suspension tube for further processing was provided. In the modality shown, the automatic suspension positioning tube and the withdrawal device 25 is movable along the rail 18 independent of the movement of the transfer device 15 in the position indicated by C suspension tubes can be empty tubes and suspension obtained with a suspension medium containing a sufficient concentration of microorganism can be delivered to the equipment for further processing, such as an incubator. Note that position C, for example, can be formed by a multichannel system which can take the positioning of the suspension tube and remove the device 25 and the transfer device 15 to a different position in which the different components are gifts or processes can be carried out.
[099] The sample suspension thus prepared is used to characterize or identify microorganisms using MALDI and optionally used for the other analysis, such as AST. For the identification of microorganisms using MALDI, a drop of said sample suspension is obtained; and that drop is transferred to said target plate. A drop can be obtained through an additional selection tool, which is maintained by means of gripping at 17 and which is automatically reduced in the suspension. When this selection tool is lifted out of the suspension a drop of suspension will stay on the tip of the selection tool, which can be transferred along the track to position B, where the selection tool with the drop is reduced until that the deposit drop contacts stain on the target plate, and at least part of the drop remains in place after the deposit of the selection tool has been lifted off the target plate. Alternatively, a pipetting tool, to be described below, can be used to select a quantity of suspension from the suspension tube, transfer this value to position B and deposit a drop of suspension on the target plate. After a drop of suspension has been deposited on the target plate, and in particular when this drop has been left to dry, a drop of a MALDI matrix solution is automatically superimposed over the amount or portion of the sample deposited on the target plate. For carrying out further tests or another analysis of a second drop of said sample suspension, it can be obtained in a similar way, and such a drop can be transferred automatically to be deposited, for example a test culture plate, which is further transferring in an automated way to carry out a susceptibility test or other additional analysis.
[0100] Each suspension tube comprises a unique identification mark, which is stored together with the properties of the suspension, with a link to the identity of the culture plate from which the selected microorganism colony was obtained in said memory central control computer for the purpose of among other things correctly and quickly linking the results obtained from the analysis with the culture plate and colony belonging to said results.
[0101] Although not shown in Figure 1, a quantity of suspension can be taken from the suspension in the suspension tube by means of a pipetting tool, which can be maintained and positioned automatically through the gripping means (which work as a support for the pipetting tool 17) and transferring or positioning the device 15, in the same way as a selection tool. The positioning device 15 is arranged to position said pipetting tool from a starting position above the suspension tube and to lower and raise the pipetting tool into and away from the suspension and for automatically positioning the pipetting tool. a transfer position, respectively. When the pipetting tool is lowered to the suspension in said suspension tube the pipetting tool is operated in a manner known per se (for example, with the use of under pressure) to select a quantity of suspension. After that, the pipetting tool with said amount of suspension is raised to the transfer position. To maintain the quantity, the pipetting tool comprises a pressurizable chamber closed by a controlled valve. The pipetting tool is automatically transferred by the transfer device 15 to position B, above, one of the target plate deposition points. In this position, the pipetting tool is lowered a predefined distance above the target plate, after which the chamber is pressurized to a pressure in the range of about 0.5 bar to 1.1 bar. The valve is then opened for such a time that a drop of suspension with a volume of a range of about 0.5 to 3.0 µl is deposited at the deposition point, in particular covering at most approximately half of said one of the target plate deposition points. After the drop has been deposited, the pipetting tool is lifted from the target plate and can be transferred to position C, where it can be discarded or cleaned for reuse.

权利要求:
Claims (20)
[0001]
1. Method, CHARACTERIZED by the fact that it comprises: providing a culture plate comprising a number of colonies of microorganisms; obtain an initial image of the culture plate including one or more colonies of microorganisms on the culture plate; select one or more colonies of microorganisms from the one or more colonies in the initial image; locate one or more of the colonies of selected microorganisms on the culture plate; provide a positioning device with a selection tool holder to hold a selection tool, the positioning device being arranged to: (a) position a selection tool above the localized colonies of microorganisms on the culture plate; (b) automatically lowering and raising a selection tool towards and away from the culture plate; and (c) positioning a selection tool in a transfer position; provide a first selection tool; position the first selection tool in the positioning device selection tool holder; obtain a first sample of at least one of the localized colonies of microorganisms with the positioning device for performing MALDI by: (a) placing the first selection tool above one of the localized colonies of microorganisms on the culture plate , (b) automatically lower the first selection tool towards the culture plate and in contact with one of the localized colonies of microorganisms in order to take a sample from one of the localized colonies of microorganisms, and (c ) automatically raise the first selection tool with the sample from one of the colonies located of microorganisms away from the culture plate to the transfer position; providing a target plate holder to support a target plate; providing a target plate having a plurality of deposition points; position the target plate in the target plate holder; deposit at least some of the first sample on at least one of the target plate deposition points with the positioning device by: (a) transferring the first transfer position selection tool to a position above one of the plate deposition points (b) lower the first selection tool so that at least some of the first sample contacts the target plate, and (c) raise the first selection tool on the target plate, at least some of the first sample is deposited on the target plate so that it covers at most approximately half of at least one of the deposition points on the target plate; optionally provide a second selection tool; optionally position the second selection tool in the positioning device selection tool holder; obtain a second sample of at least one of the localized colonies of microorganisms with the positioning device to perform a susceptibility test by: (a) positioning the first or second selection tool above one of the localized colonies of microorganisms on the culture plate, (b) automatically lower the first or second selection tool towards the culture plate and in contact with one of the localized colonies of microorganisms in order to take a sample from one of the localized colonies of microorganisms , and (c) automatically lifting the first or second selection tool with the sample from one of the colonies located of microorganisms away from the culture plate to the transfer position; provide a test culture plate; and depositing at least some of the second sample on the test culture plate.
[0002]
2. Method according to claim 1, CHARACTERIZED by the fact that it further comprises: transferring the target plate with at least some of the first sample to a device for performing MALDI for identification of the first sample; and transferring the test culture plate with at least some of the second sample to an apparatus for carrying out a susceptibility test.
[0003]
3. Method according to claim 1 or 2, CHARACTERIZED by the fact that it further comprises: superimposing a drop of a MALDI matrix solution on at least some of the first sample deposited on the target plate.
[0004]
Method according to any one of claims 1 to 3, CHARACTERIZED by the fact that the culture plate is provided with an individual identification identifying the culture plate, such as a bar code, and the method further comprising: storing ( a) the initial image of the culture plate, (b) information regarding one or more of the selected colonies of microorganisms, and (c) the identification of the culture plate in a central control computer's memory.
[0005]
5. Method according to claim 4, CHARACTERIZED by the fact that it further comprises: manually insert the processing instructions in relation to the processing to which one or more colonies of microorganisms selected from the culture plate must be subjected, and store processing instructions in the central control computer's memory.
[0006]
6. Method according to claim 4 or 5, CHARACTERIZED by the fact that the step of locating one or more of the selected microorganism colonies on the culture plate comprises: (a) positioning the culture plate on a platform comprising an imaging device, (b) obtaining an image of the culture dish, (c) obtaining the identification of said culture dish, and (d) comparing the image obtained by the imaging device with the initial stored image of the culture dish.
[0007]
7. Method according to any one of claims 1 to 6, CHARACTERIZED by the fact that the step of depositing at least some of the first sample on at least one of the deposition points of the target plate with the positioning device still comprises: moving the first selection tool in a plane parallel to the plane of the target plate after the first selection tool comes into contact with the target plate.
[0008]
8. Method according to any of claims 1 to 7, CHARACTERIZED by the fact that (a) the second selection tool is provided, (b) the second selection tool is positioned in the selection tool holder of the positioning device , and (c) the second sample is obtained with the second selection tool, not with the first selection tool.
[0009]
9. Method according to any of claims 1 to 7, CHARACTERIZED by the fact that the second sample is obtained with the first selection tool, not with the second selection tool.
[0010]
10. Method according to any one of claims 1 to 9, CHARACTERIZED by the fact that the first and second samples comprise at least one of the same colonies of microorganisms located on the culture plate.
[0011]
11. Method according to any one of claims 1 to 10, CHARACTERIZED by the fact that the first and second samples consist entirely of a single of the colonies located of microorganisms on the culture plate.
[0012]
12. Method for locating and selecting a colony of microorganisms on a culture plate and identifying microorganisms in said selected colony using MALDI, CHARACTERIZED by the fact that said method comprises the automated steps of: locating and select a colony of microorganisms on a culture plate; obtain a sample of said selected microorganism colony; and depositing at least some of said sample of said selected microorganism colony on a target plate, wherein the step of depositing at least some of said sample of said selected microorganism colony on a target plate is carried out by the automated steps of : transferring said sample obtained in a suspension tube containing a quantity of a suspension medium; preparing a suspension of the common sample by transferring at least a portion of said sample obtained in said suspension medium; obtaining a first drop of said suspension of the common sample; transferring said drop of suspension of the common sample onto said target plate; and transferring said target plate with said sample to an apparatus for performing MALDI for identification of said sample of said selected microorganism colony, in which the method further comprises the automated steps of: obtaining a second drop of said common sample suspension ; depositing said second drop of suspension of the common sample on a test culture plate; and transferring said test culture plate to an apparatus for carrying out a susceptibility test or other additional analysis, wherein the method further comprises providing an automatic suspension medium dispenser that automatically dispenses the suspension medium in the suspension tube , in which the common sample suspension is prepared by: providing a turbidity meter to perform measurements of the turbidity of a suspension medium contained in a suspension tube; provide a final measurement value indicative of the measured turbidity; and providing a controller communicatively connected to the automatic suspension medium dispenser and the turbidity meter to automatically control the operation of the automatic suspension medium dispenser and the operation of the turbidity meter, and wherein said controller compares the final measurement value at a first threshold value and, if the final measurement is above a first threshold value, the controller causes the automatic suspension medium dispenser to provide an additional amount of suspension medium in the suspension tube; and, if the final measurement is identical to, or less than, the first threshold value and identical to, or above, a second threshold value, said first threshold value being equal to or greater than the second threshold value , the controller reports that the suspension can be removed for further processing.
[0013]
13. Method according to claim 12, CHARACTERIZED by the fact that the method further comprises the automated step of superimposing a drop of a MALDI matrix solution on said drop of common sample suspension deposited on the target plate, and wherein said drop of common sample suspension deposited on the target plate is allowed to dry before said drop of MALDI matrix solution is overlaid.
[0014]
14. Method according to claim 12, CHARACTERIZED by the fact that it also comprises the automated steps of: providing a first selection tool and providing a positioning device with a selection tool holder to maintain a selection tool, said positioning device being arranged to position a selection tool in a starting position above the location obtained from the selected microorganism colony on the culture plate and to automatically lower and raise a selection tool towards, and away from, the , culture plate and to position a selection tool in a transfer position, respectively; positioning said first selection tool in said positioning device selection tool holder; by said positioning device, position the first selection tool in the starting position above the location obtained from the selected microorganism colony on the culture plate, automatically lower the first selection tool towards the culture plate in contact with the microorganism to taking a sample of said micro-organism, automatically lifting the first selection tool with said sample of the micro-organism away from the culture plate to the transfer position; provide a suspension tube support to hold a suspension tube; position a suspension tube in the suspension tube support; providing a transfer device to automatically transfer a selection tool from the transfer position of the positioning device to a position above a suspension tube held in the suspension tube holder, and to lower and raise a selection tool inward and away from a suspension medium contained in a suspension tube, and to position a selection tool in a standby position above a suspension tube maintained in the suspension tube holder, respectively; and providing a controller communicatively connected to the positioning device, the transfer device, to automatically control the movement of the positioning device and the movement of the transfer device, and the operation of the automatic suspension medium dispenser and the meter operation turbidity.
[0015]
15. Method according to claim 14, CHARACTERIZED by the fact that the method comprises providing an automatic device for positioning and removing the culture plate for positioning and removing a culture plate automatically comprising said microorganism on and from the platform, respectively, the step of arranging the controller in such a way that it is communicatively connected to the automatic device for positioning and removing the culture plate to control the operation of the automatic device for positioning and removing the culture plate, in that the stage of positioning the culture plate comprising said microorganism on the platform is carried out automatically under the control of the controller.
[0016]
16. Method according to claim 15, CHARACTERIZED by the fact that the controller is arranged in such a way that a culture plate is allowed to be automatically removed from the platform by the automatic device for positioning and removing the culture plate only after the that the suspension tube with the suspension can be removed from the suspension tube holder for further processing is provided.
[0017]
17. Method according to claim 14, CHARACTERIZED by the fact that the method comprises the provision of an automatic suspension tube positioning and removal device for positioning and automatically removing a suspension tube in and from the suspension tube support, respectively, the step of arranging the controller in such a way that it is communicatively connected to the automatic suspension tube positioning and removal device to control the operation of the automatic suspension tube positioning and removal device, in that the step of positioning a suspension tube on the suspension tube holder is performed automatically under control of the controller.
[0018]
18. Method according to claim 17, CHARACTERIZED by the fact that the controller is arranged in such a way that a suspension tube holder is automatically removed from the suspension tube holder by the automatic suspension tube positioning and removal device only after the signal that the suspension tube with the suspension can be removed from the suspension tube holder for further processing is provided.
[0019]
19. Method according to claim 14, CHARACTERIZED by the fact that the method comprises the step of providing an identification mark on the suspension tube, and the step of storing the identification mark of said suspension tube together with the properties of the suspension, with a link to the identity of the culture plate from which the selected microorganism colony was obtained in the memory of said central control computer.
[0020]
20. Method according to claim 14, CHARACTERIZED by the fact that the method comprises the provision of an automatic suspension tube positioning and removal device for automatically positioning and removing a suspension tube in and from the support the suspension tube, respectively, the step of arranging the controller in such a way that it is communicatively connected to the automatic suspension tube positioning and removal device to control the operation of the automatic suspension tube positioning and removal device, where the step of positioning a suspension tube in the suspension tube holder is performed automatically under the control of the controller.

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法律状态:
2018-12-04| B06F| Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]|

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2019-11-26| B06U| Preliminary requirement: requests with searches performed by other patent offices: procedure suspended [chapter 6.21 patent gazette]|

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2020-07-28| B09A| Decision: intention to grant [chapter 9.1 patent gazette]|

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2020-12-01| B16A| Patent or certificate of addition of invention granted|Free format text: PRAZO DE VALIDADE: 20 (VINTE) ANOS CONTADOS A PARTIR DE 02/04/2013, OBSERVADAS AS CONDICOES LEGAIS. |

优先权:

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申请号 | 申请日 | 专利标题

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US201261618003P| true| 2012-03-30|2012-03-30|

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US61/618.003|2012-03-30|

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PCT/NL2013/050239|WO2013147610A2|2012-03-30|2013-04-02|Automated selection of microorganisms and identification using maldi|

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