pharmaceutical composition comprising a complement inhibitor and an anti-th17 agent, and uses thereo

专利摘要:
PHARMACEUTICAL COMPOSITION UNDERSTANDING A COMPLEMENT INHIBITOR AND ANTI-TH17 AGENT, AND USES OF THE SAME.The present invention relates to a composition comprising a complement inhibitor and an anti-Th17 agent useful in the treatment of a chronic complement-mediated disorder or a chronic disorder of the respiratory system. In some modalities, the disorder is a chronic obstructive pulmonary disorder. In some embodiments, the disorder is asthma. The invention also relates to uses of an anti-Th17 agent and / or a complement inhibitor in the preparation of pharmaceutical compositions.
公开号:BR112013033272A2
申请号:R112013033272-7
申请日:2012-06-22
公开日:2020-11-10
发明作者:Cedric François；Pascal Deschatelets
申请人:Apellis Pharmaceuticals, Inc.；
IPC主号:

专利说明:

[002] [002] Chronic disorders of the respiratory system are important causes of morbidity and mortality, the incidence of which is increasing worldwide. According to World Health Organization estimates, about 80 million people have moderate to severe chronic obstructive pulmonary disorder (COPD), and more than 3 million people died of COPD in 2005 (~ 5% of all deaths globally). COPD was the fifth leading cause of death in 2002, and estimates suggest it will be the third leading cause of death in the world in 2030, unless the main risk factors, particularly tobacco use, can be successfully controlled . Asthma is also a significant problem for global health, which is estimated to affect 300 million individuals worldwide. Both asthma and COPD can have debilitating effects on patients' daily functioning and quality of life, particularly when they are severe. These diseases also represent significant burdens in terms of health care costs and lost productivity.
[003] [003] Pharmacological therapies such as bronchodilators and corticosteroids are widely used in the treatment of asthma and COPD. However, a significant proportion of patients experience persistent symptoms despite such interventions. In addition, these agents can be associated with significant side effects
[004] [004] The invention provides, among other things, methods to treat a chronic and complement-mediated disorder, the methods comprising administering a complement inhibitor to a subject in need of treatment for the disorder. In some aspects, the invention provides methods of treating a chronic disorder of the respiratory system, methods that comprise the administration of a complement inhibitor to a subject in need of treatment for the disorder. In some modalities, the disorder is similar. In some modalities, the disorder is COPD. Certain aspects of the invention are based, at least in part, on the recognition that complement inhibitors exhibit a prolonged duration of effect in the treatment of complement-mediated chronic disorders, for example, complement-mediated chronic respiratory system disorders, such as asthma or COPD. For example, in some modalities, the duration of action of a complement inhibitor to significantly reduce one or more manifestation (s) of a chronic complement-mediated disorder, for example, a chronic respiratory disorder, is greater than the duration of action of the complement inhibitor to substantially inhibit the complement's ability to activate plasma when administered intravenously.
[005] [005] In some respects, the invention provides a method of treating a subject in need of treatment for a chronic respiratory disorder or other complement-mediated chronic disorder, a method comprising the administration of multiple doses of a complement inhibitor to a subject according to the dosing schedule where successive doses are administered, on average, (i) at least 2 weeks after the plasma concentration of the complement inhibitor is reduced to no more than 20% of the maximum plasma concentration that has been reached after the previous dose; (ii) at least two weeks after the plasma complement activation capacity has returned to at least 50% of the baseline value after the previous 5 dose; (iii) at intervals equal to at least 2 times the terminal plasma half-life of the complement inhibitor; or (iv) at intervals of at least 3 weeks.
[006] [006] In some modalities of any method including dosage, at least 5 doses are administered.
[007] [007] In some modalities, a subject is in need of treatment for asthma, chronic obstructive pulmonary disorder (COPD) or both. In some modalities, a subject is in need of treatment for severe asthma.
[008] [008] In some embodiments, a complement inhibitor is administered via the respiratory route. In some embodiments, the complement inhibitor is administered using a nebulizer, metered dose inhaler or dry powder inhaler. In some embodiments, a complement inhibitor is administered using a vibrating mesh nebulizer.
[009] [009] In some embodiments, a complement inhibitor is administered intravenously.
[0010] [0010] In some embodiments, a complement inhibitor acts on C3 or upstream of C3 in the complement cascade. In some embodiments, the complement inhibitor inhibits cleavage of C3, C5 or factor B.
[0011] [0011] In some embodiments, a complement inhibitor comprises an antibody, aptamer, peptide, polypeptide or molecule.
[0012] [0012] In some embodiments, a complement inhibitor comprises an antibody, aptamer, peptide, polypeptide or small molecule that binds to C3, C5, factor B or factor D. 5 [0013] In some embodiments, an inhibitor of complement comprises a compstatin analogue.
[0014] [0014] In some embodiments, a complement inhibitor comprises a compstatin analogue whose sequence comprises SEQ ID NO: 14, 21, 28, 29, 32, 33, 34 or 36.
[0015] [0015] In some embodiments, a complement inhibitor comprises a compstatin analogue whose sequence comprises any of SEQ ID NO: 3 - 41.
[0016] [0016] In some modalities, a complement-mediated disorder is a disorder associated with Th17.
[0017] [0017] In some modalities, any treatment method comprises the detection of a Th17 biomarker in the subject or in a sample obtained from the subject. In some embodiments, the Th17 bi-marker is detected in a sample comprising a body fluid, in which the body fluid is optionally selected from blood, BAL fluid, sputum, nasal or urine discharge or a combination of these. In some embodiments, the biomarker comprises at least one cytokine that is produced by or promotes the formation, survival or activity of Th17 cells. In some modalities, a higher level of the Th17 biomarker compared to a reference indicates that the subject needs a dose of the complement inhibitor. In some modalities, the reference is within the normal range for personnel who do not suffer from the disorder or is a baseline value for the subject when the disorder is under control. In some embodiments, the Th17 biomarker is detected before the administration of a dose of the complement inhibitor and serves as an indicator that the subject needs a dose of the complement inhibitor. In some embodiments, the biomarker is detected before administration of a dose of the complement inhibitor and serves as an indicator that the subject needs a dose of the complement inhibitor, and the method comprises the administration of the inhibitor of the complement. complement within a predetermined period of time after detection of the biomarker. In some modes, a predetermined period of time is 1, 2, 3, 4, 5, 6, or 7 days, or 2, 3, or 4 weeks.
[0018] [0018] In some respects, a method of treating a subject that needs treatment for a chronic complement-mediated disorder comprises: (a) administration of at least one dose of a complement inhibitor to the subject; and (b) monitoring the subject for the detection of the Th17 biomarker in the subject or in a sample obtained from the subject. In some modalities, the method also comprises: (c) administration of at least one additional dose of the complement inhibitor to the subject. In some embodiments, step (b) comprises the detection of a Th17 biomarker in the subject or in a sample obtained from the subject. In some modalities, step (b) comprises the detection of a higher level of the biomarker compared to a reference, where the higher level indicates that the subject needs a dose of the complement inhibitor. In some embodiments, step (b) comprises detecting a higher level of the biomarker compared to a reference, where the higher level indicates that the subject needs a dose of the complement inhibitor, and the method comprises further (c) administration of at least one additional dose of complement inhibitor to the subject. In some embodiments, step (b) comprises detecting a higher level of the biomarker compared to a reference, where the highest level includes
[0019] [0019] In some embodiments, an anti-Th17 agent comprises an agent that inhibits the formation of or the activity of Th17 cells. In some embodiments, an anti-Th17 agent comprises an agent that inhibits the production or activity of a cytokine produced by Th17 cells or that promotes the formation or activity of Th17 cells. In some embodiments, an anti-Th17 agent comprises an agent that inhibits the production or activity of IL-1, IL-6, IL-21, IL-22, IL-17 or IL-23. In some embodiments, an anti-Th17 agent comprises an antibody, small molecule, aptamer, polypeptide or RNAi agent. In some embodiments, an anti-Th17 agent comprises an antibody, small molecule, aptamer or polypeptide that binds to IL-1, IL-6, IL-21, IL-22, IL-17 or IL-23 or binds to a receiver from any of the above.
[0020] [0020] In some respects, a pharmaceutical composition comprising a complement inhibitor and an anti-Th17 agent is provided. In some modalities in which the complement inhibitor inhibits C3 activity or C3 activation. In some embodiments, the complement inhibitor comprises a compstatin analog. In some embodiments where an anti-Th17 agent comprises an antibody, small molecule, aptamer or polypeptide that binds to IL-1, IL-6, IL-21, IL-22, IL-17 or IL-23 or connects to a receiver from any of the above.
[0021] [0021] In some ways, a method of treating a device
[0022] [0022] In some respects, a method of treating a Th17-associated disorder comprises administering a complement inhibitor and an anti-Th17 agent to a subject who needs these.
[0023] [0023] In some respects, a DC-Th17-B-Ab-C-DC cycle interruption method is provided, a method comprising administering a composition comprising a complement inhibitor and an anti-Th17 agent. for a guy who needs it.
[0024] [0024] In some respects, a method of treating a Th17-associated disorder comprises administering a complement inhibitor and an anti-Th17 agent to a subject who needs these.
[0025] [0025] In some respects, a method of treating a Th17-associated disorder comprises administering a composition comprising a complement inhibitor and an anti-Th17 agent to a subject who needs it.
[0026] [0026] In some respects, a DC-Th17-B-Ab-C-DC cycle interruption method is provided, a method comprising administering a composition comprising a complement inhibitor and an anti-Th17 agent. for a guy who needs it.
[0027] [0027] In some modalities, any of the methods includes monitoring the subject to detect evidence of a DC-Th17-B-Ab-C-DC cycle.
[0028] [0028] In some modalities, any of the methods includes monitoring the subject to detect evidence of a cycle DC-Th17-B-Ab-C-DC and the administration of a complement inhibitor, anti-Th17 agent or composition comprising a complement inhibitor, anti-Th17 agent to the subject based, at least in part, on a result of such monitoring.
[0029] [0029] In some modalities, any of the methods includes monitoring the subject for the detection of a Th17 biomarker.
[0030] [0030] In some modalities, any of the methods includes monitoring the subject for the detection of a Th17 biomarker and the administration of a complement inhibitor, anti-Th17 agent or composition comprising a complement inhibitor, anti-Th17 agent to the subject based, at least in part, on a monitoring result.
[0031] [0031] In some respects, a method of treating a subject who suffers or is at risk of suffering from a complement-mediated disorder comprises monitoring the subject for the detection of evidence of a DC-Th17-B cycle -Ab-C-DC and the administration of a complement inhibitor to the subject based, at least partially, on a result of such monitoring. In some embodiments, a method further comprises administering an anti-Th17 agent to the subject.
[0032] [0032] In some aspects, a method of treating a subject who is suffering or is at risk of suffering from a complement-mediated disorder comprises monitoring the subject for the detection of evidence of a cycle DC-Th17-B -Ab-C-DC and the administration of a complement inhibitor and an anti-Th17 agent to the subject based, at least partially, on a result of such monitoring.
[0033] [0033] In some aspects, a method of treating a subject who suffers or is at risk of suffering from a disorder associated with Th17, a method characterized by the fact of understanding the monitoring of the subject for the detection of evidence of a cycle DC-Th17-B-
[0034] [0034] In some embodiments, a method further comprises administering an anti-Th17 agent to the subject.
[0035] [0035] In some aspects, a method of treating a subject who is suffering or is at risk of suffering from a disorder associated with Th17, a method that includes monitoring the subject for the detection of evidence of a DC-cycle Th17-B-Ab-C-DC and the administration of a complement inhibitor and an anti-Th17 agent to the subject based, at least partially, on a result of such monitoring. In some embodiments, the complement inhibitor inhibits C3 activity or C3 activation. In some embodiments, the complement inhibitor comprises a compstatin analog.
[0036] [0036] In some embodiments of a composition or method related to, at least in part, an anti-Th17 agent, the anti-Th17 agent comprises an antibody, small molecule, aptamer or polypeptide that binds to IL-1, IL- 6, IL-21, IL-22, IL-17 or IL-23 or binds to a receptor from any of the above.
[0037] [0037] In some modalities of any method including monitoring the subject for the detection of evidence of a cycle DC-Th17-B-Ab-C-DC, such monitoring comprises the evaluation of a biomarker associated with Th17 in a subject or in a sample obtained from a subject.
[0038] [0038] In some modalities of any method including monitoring a subject, monitoring occurs approximately every 1-2 weeks, 2-4 weeks, or approximately every 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11 or 12 months.
[0039] [0039] In some modalities of any method comprising the administration of a complement inhibitor, anti-inflammatory agent
[0041] [0041] All articles, books, patent applications, patents, other publications, websites and databases mentioned in this application are incorporated into this document by reference. In the event of a conflict between the specification and any of the embedded references, the specification (including any changes to it) will prevail. Unless otherwise indicated, meanings of terms and abbreviations accepted in the art are used in this document. Brief Description of Drawings
[0042] [0042] Figures 1 - 11 are graphs showing concentrations in bronchoalveolar lavage fluid (BAL) of the indicated cytokines, measured in samples obtained from individual cynomolgus monkeys at the indicated points in time before or after Ascaris suum 0 challenges , 1 and 2. Control animals (blue, triangles); animals treated with Budesonide (red; +); animals treated with CA-28 (green; circles). Plots of average cytokine concentration at each point in time are overlaid and shown as continuous lines to more clearly show changes over the 24 hour time period. Detailed Description of Certain Modalities of the Invention
[0043] [0043] As used herein, the term "antibodies" encompasses antibodies and antibody fragments comprising an antigen binding site. Antibodies useful in certain modalities of the invention could be derived from or derived from various species, for example, human, non-human primate, rodent (for example, mouse, rat, rabbit), goat, chicken, and / or can be of several classes of antibodies, for example, human classes: IgG (for example, IgGl, IgG2, IgG3, IgG4), IgM, IgA, IgD and IgE. An antibody fragment (Fab) can be, for example, a Fab ', F (ab') 2, scFv (single chain variable) or another fragment that retains or contains an antigen binding site. See, for example, Allen, T., Natural Reviews Cancer, Vol.2, 750-765, 2002, and references therein. Antibodies known in the art as diabody, minibody or nanobody can be used in several modalities. Biospecific or multispecific antibodies can be used in several modalities. The light and heavy chains of IgG immunoglobulins (for example, rodent or human IgGs) contain four framework regions (FR1 to FR4) separated, respectively, by three complementarity determining regions (CDR1 to CDR3). CDRs, particularly in the CDR3 regions, especially the CDR3 heavy chain, are largely responsible for the specificity of the antibody. An antibody may be a chimeric antibody in which, for example, a variable domain of rodent or non-human primate origin is fused to a constant domain of human origin, or a "humanized" antibody, where some or all of the amino acids in the complementarity determining region (CDR) that constitute an antigen binding site (sometimes together with one or more amino acids or framework regions) are "grafted" from a rodent antibody (for example: murine antibody ) or phage display antibody to a human antibody, thus maintaining the specificity of the rodent or phage display antibody.
[0044] [0044] The terms "approximately" or "about" in reference 5 to a number generally include numbers that fall within ± 10%, in some modalities ± 5%, in some modalities ± 1%, in some modalities ± 0 , 5% of the number, unless otherwise indicated or evident otherwise through the context (except when such number inadmissibly exceeds 100% of a possible value).
[0045] [0045] "Complement activation capacity" refers to the level of complement activation that would result from exposure to a stimulus that causes maximum complement activation. Normally, the complement's ability to activate is assessed using a sample obtained from a subject (for example, a blood, plasma, serum or other fluid sample, which can be diluted properly), a sample that can be exposed in vitro a complement, activating the stimulus. A heat inactivated sample can be used as a control. It will be understood that the stimulus does not need to be sufficient to cause maximum complement activation in order to provide a measure of the complement's activation capacity. For example, to the extent that activation of the add-on occurs within a defined period of time, it can provide an indication of the ability to activate the add-on. Complement activation can be measured using, for example, an appropriate assay as a functional assay based on hemolysis (for example, lysis of red blood cells from sheep or chicken); deposition or capture of complement activation products (for example, C3a, C3b, iC3b, C5a, MAC), etc. The activation capacity of the specific complement to the pathway can be assessed using, for example, appropriate stimuli and assay conditions (for example, the presence or absence of calcium ions in the assay composition) to activate one or more of the routes. For example, antibodies (for example, IgM or immune complex) can be used to activate the classic route; lipopolysaccharide (LPS) can be used to activate the alternative pathway, mannan can be used to activate the mannose-binding lectin portion of the lectin pathway, etc. In some modalities, the total classical complement activity in a sample is measured using a CH50 test using antibody-sensitized sheep or chicken erythrocytes as the activator of the classical complement pathway and various dilutions of the test sample to determine the amount needed to give 50% Lysis. Percent hemolysis can be determined spectrophotometrically. The greater the sample dilution that can still reach 50% of lysis (that is, the more diluted the sample), the greater the activation capacity of the complement. In some embodiments, an ELI-SA based assay is used. In some embodiments, complement activation is assessed based on iC3b levels, for example, substantially as described in PCT / US2010 / 035871 (WO2010135717) (see Examples). In some modalities, complement activation is assessed based on C3b levels, for example, substantially as described in PCT / US2008 / 001483 (WO / 2008/097525), Examples 1 and 2, respectively. In some modalities, complement activation via the classic route is assessed using the MicroVue CH50 Eq EIA Kit (classic route), MicroVue Bb Plus EIA Kit (alternatively), MicroVue iC3b EIA Kit or MicroVue C3a Plus EIA Kit ( all of Quidel Corp.). In some modalities, the amount of a complement activation product is normalized to the amount of intact C3 present in the sample before exposure to a complement activation stimulus.
[0046] [0046] A "complement component" or "complement protein" is a protein that is involved in the activation of the complement system or participates in one or more complement-mediated activities. Components of the classic complement pathway include, for example, C1q, C1r, C1s, C2, C3, C4, C5, C6, C7, C8, C9 and the C5b-9 complex, also referred to as the membrane attack complex - 5 na (MAC) and active fragments or enzymatic cleavage products from any of the above (for example, C3a, C3b, C4a, C4b, C5a, etc.). Components of the alternative pathway include, for example, factors B, D and properdin. Components of the lectin pathway include, for example, MBL2, MASP-1 and MASP-2. Complement components also include cell-bound receptors for soluble complement components, where that receptor mediates one or more biological activities of such a soluble complement component following the binding of the soluble complement component. Such receivers include, for example, C5a (C5aR) receiver, C3a (C3aR) receiver, Complement Receiver 1 (CR1), Complement Receiver 2 (CR2), Complement Receiver 3 (CR3, also known as CD45), etc. It will be appreciated that the term "complement component" is not intended to include those molecules and molecular structures that serve as a "trigger" for complement activation, for example, antigen-antibody complexes, foreign structures found on microbial or artificial surfaces, etc. .
[0047] [0047] A "complement regulatory protein" is a protein involved in the regulation of complement activity. A complement regulatory protein can down-regulate complement activity by, for example, inhibiting complement activation or deactivating or accelerating the decay of one or more activated complement proteins. Examples of complement regulatory proteins include C1 inhibitor, C4 binding proteins, clusterin, vitronectin, CFH, factor I and CD46, CD55, CD59, CR1, CR2 and CR3 cell-bound proteins.
[0048] [0048] "Linked", as used here with respect to two or more moieties, means that the moieties are physically associated or linked together to form a molecular structure that is sufficiently stable for the moieties to be remain associated in the 5 conditions in which the bond is formed and, preferably, in the conditions in which the new molecular structure is used, for example, physiological conditions.
[0049] [0049] "Polypeptide", as used herein, refers to a polymer of amino acids, optionally including one or more amino acid analogs. A protein is a molecule composed of one or more polypeptides. A peptide is a relatively short polypeptide, typically between 2 and 60 amino acids in length, for example, between 8 and 40 amino acids in length. The terms "protein", "polypeptide" and "peptide" can be used interchangeably. Polypeptides used in this document may contain amino acids such as those that are naturally found in proteins, amino acids that are not naturally found in proteins and / or analogues of amino acids other than amino acids. As used here, an "analog" of an amino acid can be a different amino acid that structurally resembles amino acids, or a compound that is not an amino acid that structurally resembles the amino acid. A large number of analogs known in the art of the 20 amino acids commonly found in proteins are known (the "standard" amino acids). One or more of the amino acids in a polypeptide can be modified, for example, by adding a chemical entity such as a group of carbohydrates, a phosphate group, a farnesyl group, an isopharnesyl group, a group of fatty acids, a binder for conjugation, functionalization, or other modification, etc. Certain suitable non-restrictive analogs and modifications are described in WO2004026328 and / or below. The polypeptide can be acetylated, for example, the N-terminal and / or amidated, for example, at the C-terminal.
[0050] [0050] In general, polypeptides can be obtained or produced using any suitable method, known in the art. For example, polypeptides can be isolated from natural sources, produced in vitro or in vivo using recombinant DNA technology 5 in suitable expression systems (for example, by recombinant host cells or transgenic non-human animals or plants), synthesized by chemical means such as solid phase peptide synthesis and / or using methods involving chemical bonding of the synthesized peptides (See, for example, Kent, S., J PeptSci., 9 (9): 574-93, 2003 and US Pub. No. 20040115774), or a combination thereof. An individual skilled in the art would readily select appropriate method (s). A polypeptide can include a tag, for example, an epitope tag, which can facilitate the purification and / or detection of the polypeptide. Exemplary tags include, for example, 6XHis, HA, Myc, SNUT, FLAG, TAP, etc. In some embodiments, a tag is cleavable, for example, the tag comprises a recognition site for cleavage by a protease, or is separated from an inhibitory portion of the polypeptide complement by a binding portion that comprises a recognition site for protease cleavage. For example, a TEV protease cleavage site can be used.
[0051] [0051] "Poxvirus" refers to a family of complex double-stranded DNA viruses that make up the Poxviridae family. The family includes the orthopoxvirus, a genus of the family Poxviridae, subfamily Chorodopoxvirinae, which includes many species infecting mammals, including humans. Poxviruses are described in Fields, BN, et al., Philosophy Virology, 3rd ed., Lippincott Williams & Wilkins, 2001. Orthopoxviruses include, but are not limited to, vaccinia virus, major smallpox virus, minor pox virus , cowpox virus, monkey pox virus, camel pox virus, swinepox virus and ectromelia virus.
[0052] [0052] "Poxvirus complement control protein" refers to members of a family of homologous proteins encoded by a number of different poxviruses that bind to one or more proteins in the complement pathway and inhibit the classical pathway of complement activation, the alternative complement activation pathway, the lectin pathway or any combination of these. Poxvirus complement control proteins are members of the complement control protein (CCP), also called the complement activation regulator superfamily (RCA) (Reid, KBM and Day, AJ, Immunol Today, 10: 177 -80, 1989).
[0053] [0053] "Recombinant host cells", "host cells" and other such terms, denote prokaryotic or eukaryotic cells or cell lines that contain an exogenous nucleic acid (usually DNA) as an expression vector comprising a nucleic acid cell that encodes a polypeptide of interest. It will be understood that such terms include the descendants of the original cell (s) into which the vector or other nucleic acid has been introduced. Suitable host cells include any of those routinely used in the art to express polynucleotides (for example, for the production of polypeptide (s) encoded by such polynucleotides) including, for example, prokaryotes, such as E. coli; and eukaryotes, including, for example, fungi, such as yeast (for example, Pichia pastoris); insect cells (for example, Sf9), plant cells and animal cells, for example, mammalian cells like CHO, R1.1, BW, LM, African green monkey kidney cells (for example, COS- 1, COS-7, BSC-1, BSC-40 and BMT-10) and human cell culture. The exogenous nucleic acid can be kept stable as an episome like a plasmid or can, at least in part, be integrated into the host cell genome, optionally after having
[0054] [0054] "Reactive functional groups" as used herein refers to groups, including, but not limited to, olefins, acetylenes, alcohols, phenols, ethers, oxides, halides, aldehydes, ketones, carboxylic acids, 5 esters, amides, cyanates, isocyanates, thiocyanates, isothiocyanates, amines, hydrazines, hydrazones, hydrazides, diazo, diazonium, nitro, nitriles, mercaptans, sulfides, disulfides, sulfoxides, sulfones, sulfonic acids, sulfinic acids, acetals, acetals, anhydrides, sulfates, sulfenic acids, isonitriles, amidines, imides, imidates, nitrones, hydroxylamines, oximes, hydroxamic acids, thiohydroxamic acids, alenes, orthoesters, sulfites, enamines, inamines, ureas, isreas, semicarbazides, carbodiimides, carbamates, imine , azo-compounds, azo compounds and nitrous compounds, N-Hydroxysuccinimide esters, maleimides, sulfhydryls and the like. Methods for preparing each of these functional groups are well known in the art and their application or modification for a specific purpose is within the ability of an individual skilled in the art (see, for example, Sandler and Karo, eds. ORGANIC FUNCTIONAL GROUP PREPARATIONS, Academic Press, San Diego, 1989, and Hermanson, G., Bioconjugate Techniques, 2nd ed., Academic Press, San Diego, 2008).
[0055] [0055] "Specific binding" generally refers to a physical association between a target polypeptide (or, more generally, a target molecule) and a binding molecule such as an antibody or ligand. The association is generally dependent on the presence of a specific structural characteristic of the target, such as an antigenic determinant, epitope, pocket or binding cavity, recognized by the binding molecule. For example, if an antibody is specific for epitope A, the presence of a polypeptide containing epitope A or the presence of unmarked free A in a reaction containing both unmarked free A and the binding molecule that binds to it will reduce the amount
[0057] [0057] "Treat", as used here when it comes to treating a subject, refers to the provision of treatment, that is, provision of any type of medical or surgical management of a subject. Treatment can be provided to reverse, alleviate, inhibit progression, prevent or reduce the likelihood of a disease, or to reverse, alleviate, inhibit or prevent progression, prevent or reduce the likelihood of one or more more symptoms or manifestations of an illness. "Avoid" refers to preventing a disease or symptom or manifestation of a disease from occurring for at least a period of time in at least some individuals, for example, individuals at risk of developing the disease, symptom, or manifestation. Treating may include administration of a compound or composition to the subject after the development of one or more symptoms or manifestations indicative of a disease, for example, in order to reverse, alleviate, reduce the severity of, and / or inhibit or prevent progression of the disease and / or to reverse, relieve, reduce the severity of, and / or inhibit or one or more symptoms or manifestations of the disease. A compound or composition can be administered to a subject who has developed a disease, or is at an increased risk of developing the disease in relation to a member of the general population, optionally matched in terms of age, sex and / or other variable (s) ( demographic (s).
[0058] [0058] The "variant" of a specific polypeptide or polynucleotide has one or more changes (for example, additions, substitutions and / or exclusions, which may be referred to collectively as "mutations") in relation to the polypeptide or acid nucleic, which can be referred to as the "original polypeptide" or "original polynucleotide", respectively. Thus, a variant can be shorter or longer than the polypeptide or polypeptide of which it is a variant. The term "variant" encompasses "fragments". A "fragment" is a continuous portion of a polypeptide that is shorter than the original polypeptide. In certain embodiments of the invention, a variant polypeptide has significant sequence identity with the original polypeptide over a continuous portion of the variant comprising at least 50%, preferably at least 60%, at least 70%, at least 80 %, at least 90%, at least 95%, or more, of the length of the variant or the length of the polypeptide, (whichever is shorter). In certain embodiments of the invention, a variant polypeptide has substantial sequence identity with the original polypeptide over a continuous portion of the variant that comprises at least 50%, preferably at least 60%, at least 70%, at least 80%, at least 90%, at least 95%, or more, of the length of the variant or the length of the polypeptide, (whichever is shorter). In a non-restrictive modality, a variant has at least 80% identity with the original sequence over a continuous portion of the variant comprising between 90% and 100% of the length of the variant or the length of the polypeptide, (whichever is shorter). In another non-restrictive modality, a variant has at least 80% identity with the original sequence over a continuous portion of the variant comprising between 90% and 100% of the length of the variant or the length of the polypeptide, (whichever is shorter). In specific embodiments, the sequence of a variant polypeptide has differences in amino acid N with respect to an original sequence, where N is any integer between 1 and 10. In other specific embodiments, the sequence of a variant polypeptide has amino acid differences N with respect to an original sequence, where N is any integer between 1 and 20. An amino acid "difference" refers to a substitution, insertion or deletion of an amino acid.
[0059] [0059] In certain embodiments of the invention, a fragment or variant has sufficient structural similarity to the original polypeptide so that when its three-dimensional structure (structure is real or predicted) is superimposed on the structure of the original polypeptide, the volume of overlap is at least at least 70%, preferably at least 80%, more preferably at least 90% of the total structure volume of the original polypeptide. A complete or partial three-dimensional structure of the fragment or variant can be determined by crystallization of the protein, which can be done using standard methods.
[0060] [0060] In many modalities, one, more than one, or all the biological functions or activities of a variant or fragment are substantially similar to those of the corresponding biological function or the activity of the original molecule. In certain modalities, the activity of a variant or fragment can be at least 20%, at least 50%, at least 60%, at least 70%, at least 80% or at least 90% of the molecule's activity up to about 100%, about 125%, or about 150% of the activity of the original molecule. In certain embodiments, an activity of a variant or fragment is such that the amount or concentration of the variant required to produce an effect is within 0.5 to 5 times the amount or concentration of the original molecule needed to produce that effect. The invention contemplates the use of variants of any of the complement inhibitor polypeptides disclosed in this document, wherein the variant inhibits complement sufficiently to be useful in a method described in this document. In some modalities, a variant does not have or has a substantial reduction in a property that may be undesirable as an immunogenicity.
[0061] [0061] As used herein, "alkyl" refers to a linear, branched or cyclic saturated hydrocarbon having from about 1 to about 22 carbon atoms (and all combinations and subcomponents)
[0062] [0062] As used here, "halo" refers to F, Cl, Br or I.
[0063] [0063] As used here, "alkanoyl" refers to an optionally substituted, linear or branched acyclic aliphatic residue having about 1 to 10 carbon atoms (and all combinations and subcombination of specific ranges and numbers of carbon atoms) in it, for example, from about 1 to 7 carbon atoms which, as will be appreciated, are attached to a C = O terminal group with a single bond (and can also be referred to as a " acyl group "). Alkaline groups include, but are not limited to, formyl, acetyl, propionyl, butyryl, isobutyryl, pentanoyl, isopentanoyl, 2-methyl-butyryl, 2,2-dimethoxypropionyl, hexanoyl, heptanoyl, octanoyl and the like, and for the purposes of this invention, a formyl group is considered an alkanoyl group. "Lower alkanoyl" refers to an optionally substituted, linear or branched acyclic aliphatic residue having about 1 to about 5 carbon atoms (and all combinations and subcombination of specific ranges and numbers of carbon atoms) . Such groups include, among others, formyl, acetyl, propionyl, butyryl, isobutyryl, pentanoyl, isopentanoyl, etc.
[0064] [0064] As used here, "aryl" refers to a mono- or bicyclic aromatic ring system, optionally substituted, having from about 5 to about 14 carbon atoms (and all combinations and subcombination of ranges and specific numbers of carbon atoms in it), preferably with about 6 to 10 carbons. AND-
[0065] [0065] As used in this document, "aralkyl" refers to alkyl radicals with aryl substituent and having from about 6 to about 22 carbon atoms (and all combinations and subcombination of specific intervals and numbers of carbon atoms in it), with about 6 to about 12 carbon atoms being preferable in certain modalities. Aralkyl groups can be optionally substituted. Non-restrictive examples include, for example, benzyl, naphthylmethyl, diphenylmethyl, triphenylmethyl, phenylethyl and diphenylethyl.
[0066] [0066] In this document, the terms "alkoxy" and "alkoxy" refer to an optionally substituted alkyl-O- group where alkyl is as previously defined. Exemplary alkoxy and alkoxy groups include methoxy, ethoxy, n-propoxy, i-propoxy, n-butoxy and heptoxy.
[0067] [0067] As used in this document, "carboxy" refers to a -C (= O) OH group.
[0068] [0068] As used in this document, "alkoxycarbonyl" refers to a -C (= O) O-alkyl group, where alkyl is defined as above.
[0069] [0069] As used in this document, "aroíla" refers to a group at -C (= O) -aryl, where aryl is defined as before. Exemplary aromatic groups include benzoyl and naphthyl.
[0070] [0070] The term "cyclic ring system" refers to an aromatic or non-aromatic ring system, partially unsaturated or totally saturated, with 3 to 10 members, which includes unique rings with 3 to 8 atoms and ring systems and bi- and tri-cyclic which may include aromatic aryl with 5 or 6 members or aromatic heterocyclic groups fused to a non-aromatic ring. These heterocyclic rings include those with 1 to 3 heteroatoms selected independently from the group consisting of oxygen, nitrogen and sulfur. In certain embodiments, the term heterocyclic refers to a 5, 6, or 7-membered non-aromatic ring or polycyclic group in which at least one atom of the ring is a heteroatom selected from the group consisting of O, S and N, including , among others, a bi or tri-cyclic group, comprising six-membered rings fused to between one and three heteroatoms independently selected from the group consisting of oxygen, sulfur and nitrogen. In some embodiments, "cyclic ring system" refers to a cycloalkyl group which, in this document, refers to groups having 3 to 10, for example, 4 to 7 carbon atoms. Cycloalkyls include, but are not limited to, cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, cycloheptyl and the like, which is optionally substituted. In some modalities, "cyclic ring system" refers to a portion of cycloalkenyl or cycloalkynyl, which is optionally substituted.
[0071] [0071] Typically, substituted chemical moieties include one or more substituents that replace hydrogen. X-exemplary substituents include, for example, halo, alkyl, cycloalkyl, aralkyl, aryl, sulfhydryl, hydroxyl (-OH), peroxyl, cyano (-CN), carboxyl (-COOH), -C (= O) O-alkyl , aminocarbonyl (-C (= O) NH2), aminocarbonyl - N-substituted (-C (= O) NHR "), CF3, CF2CF3, and the like. Regarding the aforementioned substituents, each R" of the portion can be, independently, any H, alkyl, cycloalkyl, aryl or aralkyl, for example.
[0072] [0072] As used herein, "L-amino acid" refers to any of the naturally occurring levorotatory alpha-amino acids normally present in proteins or the alkyl esters of such alpha-amino acids. The term "D-amino acid" refers to dextrorotatory alpha-amino acids. Unless otherwise specified, all amino acids referred to in this document are L-amino acids.
[0073] [0073] As used in this document, an "aromatic amino acid" is an amino acid comprising at least one aromatic ring, for example, comprising an aryl group.
[0074] [0074] As used herein, an "aromatic amino acid analog" is an amino acid analog that comprises at least one aromatic ring, for example, comprising an aryl group. II. Methods of Treating Disorders Using Complement Inhibitors
[0075] [0075] The present invention provides, among other things, methods of treating complement-mediated chronic disorders using complement inhibitors. For example, the invention provides methods of treating chronic respiratory diseases using complement inhibitors. In some respects, inventive methods are based, at least in part, on the recognition that complement inhibitors have a prolonged duration of action in the treatment of a variety of diseases, for example, chronic respiratory diseases, compared, for example, with its plasma half-life and / or its duration of action to inhibit the plasma complement's activation capacity. In some respects, the invention provides methods of treating a chronic complement-mediated disorder by administering multiple doses of a complement inhibitor, where the complement inhibitor is administered according to a dosing schedule that uses the prolonged effect of complement inhibition.
[0076] [0076] As used here, a "chronic disorder" is a disorder that persists for at least 3 months and / or is accepted in the art as a chronic disorder. In many modalities, a chronic disorder persists for at least 6 months, for example, at least 1 year or more, for example, indefinitely. An individual skilled in the art will appreciate that at least some manifestations of various chronic disorders may be intermittent and / or may present severe relapses and relapses over time. A chronic disorder can be progressive, for example, it has a tendency to become more severe or affect larger areas over time.
[0077] [0077] In some modalities, a chronic complement-mediated disorder is a disorder associated with Th2. As used here, a Th2-associated disorder is a disorder characterized by an excessive number and / or excessive or inadequate activity of CD4 + Th2 subtype helper T cells ("Th2 cells") in the body or a part of it, for example, at least one tissue, organ or structure. For example, there may be a predominance of Th2 cells over CD4 + T helper cells of the Th1 subtype ("Th1 cells"), for example, at least one tissue, organ or structure affected by a disorder. As known in the art, Th2 cells normally secrete characteristic cytokines, such as interleukin-4 (IL-4), interleukin-5 (IL-5) and interleukin-13 (IL-13), while Th1 cells normally secrete interferon- (IFN-) and tumor necrosis factor β (TNF β). In some modalities, a disorder associated with Th2 is characterized by the production and / or excessive amount of IL-4, IL-5 and / or IL-13, for example, in relation to IFN- and / or TNFβ, for example, at least a little, at least one tissue, organ or structure.
[0078] [0078] In some modalities, a chronic complement-mediated disorder is a disorder associated with Th17. As used here, a Th17-associated disorder is a disorder characterized by an excessive number and / or excessive or inadequate activity of CD4 + helper T cells of the Th17 subtype ("Th17 cells") in the body or a part of it, for example, by least one tissue, organ or structure. For example, there may be a predominance of Th17 cells over Th1 and / or TH2 cells, for example, at least one tissue, organ or structure affected by a disorder.
[0079] [0079] In some aspects, the present disclosure provides the view that the activation of complement and Th17 cells participate in a cycle that involves antibodies and dendritic cells and that contributes to the maintenance of a pathological immune microenvironment underlying a variety of diseases. Without wishing to be bound by any theory, the pathological immune microenvironment, once established, is self-sustaining and contributes to the damage of cells and tissues. Dendritic cells (DCs) are a type of white blood cell that occurs in most tissues in the body, particularly those exposed to the external environment, such as skin and mucous membranes and in the blood (where they can be found in an immature state). Immature DCs provide samples of the environment for pathogens 5 through, for example, standard recognition receptors, such as toll-type receptors (TLRs). In response to various stimuli (for example, substances associated with the pathogen or other danger signs, inflammatory cytokines and / or T cells activated by antigen), DCs mature and migrate to lymphoid tissues, where they act as cells present antigen and activate other cells of the immune system, such as T cells and B cells, presenting them with antigen fragments together with co-stimulation molecules not specific to the antigen. DC stimulation promotes the proliferation of Th cells, activation and differentiation in effector Th cells. Effector Th cells "help" cytotoxic T cells, B cells and macrophages, for example, by secreting cytokines that have various stimulatory effects. The aid of th can, for example, increase the proliferation and activation of cytotoxic T cells, stimulate cell proliferation and B maturation and antibody production. Of particular importance, in accordance with certain aspects of this disclosure, mature DCs are able to cause CD4 + T helper cells to differentiate into Th17 cells, which in turn stimulate B cell maturation and activation, resulting in the production of antibodies .
[0080] [0080] The antibody response is generally polyclonal, with most antibodies having low affinity. However, some of these antibodies may cross-react with autoproteins, such as enzymatically or non-enzymatically chemically modified autoproteins in the body post-translationally in any of a variety of ways. These self-proteins can,
[0081] [0081] Modified autoproteins (for example, proteins modified by malondialdehyde, proteins modified by CEP) may contain epitopes recognized as non-auto by the immune system, for example, by antibodies.
[0082] [0082] The effector pathways that lead this cycle to inflict tissue damage can be varied, but, without wanting to be linked by any theory, it is believed that a major pathway is through macrophages. In some aspects, IL-17 secreted by Th17 cells, either alone or in combination with one or more other cytokines such as gamma interferon (IFN-γ) contributes to the activation of macrophages and / or polarization towards an M1 phenotype. Macrophages polarized with M1 are immune effector cells that are characterized by the expression of high levels of proinflammatory cytokines, high production of reactive nitrogen and oxygen intermediates and can show strong cytotoxic activity against targets, such as microbes and tumor cells . Macrophages, for example, macrophages polarized with M1 and the products they produce can lead to tissue damage and are important mediators of immunopathology. Modification of autoproteins and other cellular components by reactive nitrogen and oxygen species can make them dysfunctional, thereby interfering with normal cellular processes. Modified dysfunctional proteins can accumulate to toxic levels, which can lead to cell death. Macrophages are also capable of directly killing altered auto cells, for example, auto cells that have oxidatively modified proteins or lipids exposed on their cell surface. Reactive nitrogen and oxygen species produced by macrophages can amplify oxidative stress, resulting in additional modification of autoproteins by mechanisms such as those described above, which produce new targets for auto-reactive antibodies and macrophages. The antibodies also activate the complement, which maintains the DC polarization for a Th17 promoter phenotype. Thus, a vicious cycle is perpetuated in which Th17 cells activate B cells, resulting in the production of polyclonal antibodies and consequent activation of the complement, which, in turn, promotes DC polarization to a Th17 promoter phenotype that drives the continuous stimulation of B lymphocytes and production of antibodies. For the purposes of this document, this cycle, also summarized above, can be referred to as the "dendritic cell-Th 17 cell - B cell-antibody-complement-dendritic cell" cycle, abbreviated as DC-Th17-B-Ab cycle. -C-DC. Polarization of macrophages to an M1 phenotype and production of ROS that can directly damage cellular components can occur as "exits" from this feedback loop. The pathological consequences that result from the DC-Th17-B-Ab-C-DC cycle and its outputs can vary in different tissues or organs. For example, in the respiratory system, they may at least in part underlie chronic respiratory diseases, such as asthma and COPD. In the eyes, they can, at least in part, support chronic disorders such as age-related macular degeneration. On the skin, they can at least partially support psoriasis. In the pancreas, they can at least partially support type I diabetes.
[0083] [0083] In some modalities, a chronic complement-mediated disorder is a disorder associated with IgE. As used here, an "IgE-associated disorder" is a disorder characterized by the production and / or excessive and / or inadequate amount of IgE, excessive or inadequate activity of IgE-producing cells (for example, IgE-producing B cells or plasma cells), and / or excessive and / or inadequate activity of IgE-sensitive cells such as eosinophils or mast cells. In some modalities, a disorder associated with
[0084] [0084] In some modalities, a complement-mediated chronic disorder 5 is characterized by complement-mediated hemolysis, for example, complement-mediated hemolysis attributable to the deficiency or mutation of one or more endogenous complement-regulating proteins. In some modalities, a chronic complement-mediated disorder is not characterized by attributable hemolysis, for example, deficiency or mutation of one or more endogenous complement-regulating proteins.
[0085] [0085] In some modalities, a chronic complement-mediated disorder is characterized by the presence of autoantibodies and / or immune complexes in the body, which can activate the complement through, for example, the classical route. Auto-antibodies can, for example, bind to auto antigens, for example, in cells or tissues in the body. In some modalities, autoantibodies bind to antigens in blood vessels, skin, nerves, muscles, connective tissue, heart, kidney, thyroid, etc. In some modalities, a chronic mediated complement disorder is not characterized by autoantibodies and / or immune complexes.
[0086] [0086] In some embodiments, the invention provides methods of treating a chronic complement-mediated disorder by administering multiple doses of a complement inhibitor, in which the complement inhibitor is administered according to a dosage schedule using the prolonged effect of complement inhibition. "Dose schedule" refers to the time of administration of a compound (or composition containing a compound). In some modalities, an inventive method uses a larger dosage range, compared, for example, with a dosage range that aims to maintain a significant level of complement inhibitor and / or a significant level of complement inhibition in the body substantially over a treatment period. In some embodiments, an inventive method uses a larger dosage range, in comparison, 5 for example, with a dosage range that aims to expose tissue (s) or organ (s) affected by a complement-mediated disorder to a level significant complement inhibitor and / or maintain a significant level of complement inhibition in such tissue (s) or organ (s) (and / or in contact with body fluids or within such tissue (s) or organ (s) ) substantially over a treatment period. As used herein, the "dosing schedule" refers to the time interval between administration of successive doses of a compound (or composition that comprises a compound).
[0087] [0087] In some modalities, a chronic complement-mediated disorder is a respiratory disorder. In some modalities, a chronic respiratory disorder is asthma or chronic obstructive pulmonary disease (COPD). In some modalities, a chronic respiratory disorder is pulmonary fibrosis (for example, idiopathic pulmonary fibrosis), radiation-induced lung injury, allergic bronchopulmonary aspergillosis, hypersensitivity pneumonitis (also known as allergic alveolitis), eosinophilic pneumonia , interstitial pneumonia, sarcoidosis, Wegener's granulomatosis or obliterating bronchiolitis.
[0088] [0088] In some modalities, a chronic complement-mediated disorder is allergic rhinitis, rhinosinusitis or nasal polyposis. In some embodiments, the invention provides a method of treating a subject in need of treatment for allergic rhinitis, rhinosinusitis or nasal polyposis, the method which comprises administering a complement inhibitor according to a dosage schedule described here for a subject who needs treatment for the disorder.
[0089] [0089] In some modalities, a disorder mediated by a chronic complement is a disorder that affects the musculoskeletal system. Examples of such inflammatory diseases are joint problems (eg, arthritis, such as rheumatoid arthritis or post-arthritis, chronic juvenile arthritis, spondyloarthropathies Reiter's syndrome, gout). In some modalities, a disturbance of the musculoskeletal system results in symptoms such as pain, stiffness and / or limited movement of the affected body part or parts. Inflammatory myopathies include dermatomyositis, polymyositis, and several other disorders of chronic muscle inflammation of unknown etiology that result in muscle weakness. In some modalities, a chronic complement-mediated disorder is myasthenia gravis. In some modalities, the invention provides a method of treating any of the above diseases affecting the musculoskeletal system, the method which comprises administering a complement inhibitor according to a dosing schedule described here for a subject in need of treatment for the disorder.
[0090] [0090] In some modalities, a disorder mediated by a chronic complement is a disorder that affects the integumentary system. Examples of such diseases include, for example, atopic dermatitis, psoriasis, pemphigus, lupus erythematosus, dermatomyositis, scleroderma, sclerodermatomyositis, Sjögren's syndrome and chronic urticaria. In some respects, the invention provides a method of treating any of the above diseases affecting the integumentary system, the method of administering a complement inhibitor according to a dosage schedule described here for a subject who needs treatment for the disorder.
[0091] [0091] In some modalities, a chronic complement-mediated disorder affects the nervous system, for example, the central nervous system (CNS) or peripheral nervous system (PNS). Examples of such disorders include, for example, multiple sclerosis, other chronic demyelinating diseases, amyotrophic lateral sclerosis, chronic pain, stroke, allergic neuritis, Huntington's disease, Alzheimer's disease and Parkinson's disease. In some fashion, the invention provides a method of treating any of the above diseases affecting the nervous system, the method comprising administering a complement inhibitor according to a dosing schedule described here for a subject who needs treatment for the disorder.
[0092] [0092] In some modalities, a chronic complement-mediated disorder affects the circulatory system. For example, in some modalities the disorder is vasculitis or another disorder associated with inflammation of vessels, for example, blood vessels and / or inflammation of lymphatic vessels. In some modalities, a vasculitis is polyarteritis nodosa, Wegener's granulomatosis, giant cell arteritis, Churg-Strauss syndrome, microscopic polyangiitis, Henoch-Schönlein purple, Takayasu's arteritis, Kawasaki's disorder or Behçet's disease. In some modalities, a subject, for example, a subject who needs treatment for vasculitis, is positive for anti-neutrophil cytoplasmic antibodies (ANCA).
[0093] [0093] In some modalities, a chronic complement-mediated disorder affects the gastrointestinal system. For example, the disorder can be inflammatory bowel disease, for example, Crohn's disorder or ulcerative colitis. In some embodiments, the invention provides a method of treating a complement-mediated chronic disorder affecting the gastrointestinal system, the method of administering a complement inhibitor according to a dosing schedule described here for a subject who needs it. treatment for the disorder.
[0094] [0094] In some modalities, a chronic complement-mediated disorder is thyroiditis (for example, Hashimoto's thyroiditis, Graves' disorder, postpartum thyroiditis), myocarditis, hepatitis (for example, hepatitis C), pancreatitis, glomerulonephritis (for example, membranoproliferative glomerulonephritis or glomerulonephritis membrane) or panniculitis.
[0095] [0095] In some embodiments, the invention provides methods of treating a subject suffering from chronic pain, methods which comprise the administration of a complement inhibitor to a subject according to a dosing schedule of the present invention. In some modalities, a subject suffers from neuropathic pain. Neuropathic pain has been defined as pain initiated or caused by a primary injury or dysfunction of the nervous system, in particular, pain that arises as a direct consequence of an injury or disorder that affects the somatosensory system. For example, neuropathic pain can arise from lesions that involve the somatosensory pathways with damage to small fibers in peripheral nerves and / or to the spinal-thalamocortical system in the CNS. In some modalities, neuropathic pain results from autoimmune disorder (eg, multiple sclerosis), metabolic diseases (eg, diabetes), infection (eg, viral disorder such as herpes or HIV), vascular disorder ( for example, stroke), trauma (for example, injury, surgery) or cancer. For example, neuropathic pain may be pain that persists after a wound heals or after the cessation of a peripheral nerve end stimulus or pain that arises due to nerve damage. Exemplary conditions associated with or associated with neuropathic pain include painful diabetic neuropathy, post-herpetic neuralgia (eg, persistent or recurrent pain at the site of acute herpes zoster 3 or more months after the acute episode), trigeminal neuralgia, cancer-related neuropathic pain, neuropathic pain associated with chemotherapy, HIV-related neuropathic pain (eg
[0096] [0096] In some modalities, a chronic complement-mediated disorder is a chronic eye disorder. In some modalities, chronic eye disorder is characterized by macular degeneration, choroidal neovascularization (CNV), retinal neovascularization (RNV), ocular inflammation or any combination of the above. Macular degeneration, CNV, RNV and / or eye inflammation can be a defining and / or diagnostic feature of the disorder. Exemplary diseases that are characterized by one or more of these characteristics include, but are not limited to, conditions related to macular degeneration, diabetic retinopathy, retinopathy of prematurity, proliferative vitreoretinopathy, uveitis, keratitis, conjunctivitis and scleritis . Conditions related to macular degeneration include, for example, age-related macular degeneration (AMD). In some modalities, a subject is in need of treatment for wet D-MRI. In some modalities, a subject is in need of treatment for dry AMD. In some modalities, a subject is in need of treatment for geographic atrophy (GA). In some modalities, a subject is in need of treatment for eye inflammation. Eye inflammation can affect a large number of eye structures such as the conjunctiva (conjunctivitis), cornea (keratitis), episclera, sclera (scleritis), uveal tract, retina, vasculature, or optic nerve. Evidence of eye inflammation may include the presence of inflammation-associated cells such as white blood cells (eg, neutrophils, macrophages) in the eye, the presence of endogenous inflammatory mediator (s), an or more symptoms such as eye pain, redness, sensitivity to light, blurred vision and flying flies, etc. Uveitis is a general term that refers to inflammation of the uvea of the eye, for example, in any of the structures of the uvea, including the iris, ciliary or choroidal body. Specific types of uveitis include iritis, iridocyclitis, cyclitis, pars planite and choroiditis. In some ways, a subject is in need of treatment for geographic atrophy (GA). In some embodiments, chronic eye disorder is an eye disorder characterized by damage to the optic nerve (eg, optic nerve degeneration), such as glaucoma.
[0097] [0097] In some modalities, a chronic complement-mediated disorder is chronic rejection of a transplanted organ, tissue, cells or cell populations (collectively "grafts"). Examples of grafts include, for example, solid organs such as kidney, liver, lung, pancreas, heart; tissues such as cartilage, tendons, cornea, skin, heart valves and blood vessels; pancreatic islets or islet cells. Transplant rejection is one of the greatest risks associated with transplantation between genetically different individuals of the same species (allografts) or between individuals of different species (xenografts) and can lead to failure and the need to remove the graft from the graft recipient . As used here, "chronic rejection" refers to rejection occurring at least 6 months post-transplant, for example, between 6 months and 1, 2, 3, 4, 5 years or more post-transplant, often after from months to years of good graft function. For the purposes of this, chronic rejection may include chronic graft vasculopathy, a term used to refer to fibrosis of the internal blood vessels in the transplanted tissue. In some embodiments, the invention provides a method of treating a subject in need of treatment to inhibit chronic graft disorder rejection, the method of administering a complement inhibitor to the subject according to a dosage schedule described here . In some embodiments, the invention provides a method of treating a subject who has undergone a transplant or is scheduled to undergo a transplant within the next 12 weeks. In some modalities, treatment is started no later than 1, 2, 3, 6, or 12 months after transplantation.
[0098] [0098] In some respects, the invention provides a method of treating a subject in need of treatment for a chronic complement-mediated disorder, for example, chronic respiratory disorder, a method comprising administering multiple doses of a complement inhibitor to a subject according to the dosing schedule in which successive doses are administered, on average, (i) at least 2 weeks after the plasma concentration of the complement inhibitor is reduced to no more than 20% of the maximum plasma concentration that was reached after the previous dose; (ii) at least two weeks after the plasma complement activation capacity has returned to at least 50% of the baseline value or within the normal range after the previous dose; (iii) at intervals equal to at least 2 times the terminal plasma half-life of the complement inhibitor; or (iv) at intervals of at least 3 weeks. In some embodiments, an inventive method comprises administering a complement inhibitor with an average dosage interval of at least 3 weeks, for example, between 3 and 15 weeks, for example, between 3 and 12 weeks, for example, between 3 and 10 weeks, for example, between 4 and 8 weeks, for example, about every 4, 5, 6, 7, or 8 weeks.
[00100] [00100] In some embodiments, an inventive method comprises the administration of a complement inhibitor with an average dosage interval of at least 3 weeks, for example, between 3 and 15 weeks, for example, between 3 and 12 weeks, for example, between 3 and 10 weeks, for example, between 4 and 8 weeks, for example, about every 4, 5, 6, 7, or 8 weeks. In some embodiments, an inventive method comprises administering a complement inhibitor with an average dosage interval of between 4 and 6 weeks. In some modalities, a sufficient dose is administered to substantially inhibit the ability to activate the plasma complement. In some modalities, a sufficient dose is administered to significantly inhibit the ability to activate the local complement in a tissue or organ affected by a complement-mediated disorder. In some embodiments, complement activation capacity, for example, plasma complement activation capacity or local complement activation capacity, is considered "substantially inhibited" if reduced to no more than twice normal levels, for example , at approximately normal levels. Normal levels (for example, for any aspect or embodiment of the invention) can be the levels determined using a variety of suitable approaches. For example, a control sample 5, for example, a plasma control sample or other body fluid sample, in which the complement was inactivated, for example, by heat inactivation, or which was depleted by one or more complement components such as C3 can be used, and / or a control test can be performed in which an essential test component is omitted. In some modalities, a sufficient dose to reduce and / or maintain the plasma complement within the normal range administered. In some modalities, a sufficient dose is administered to reduce and / or maintain activation of the local complement in a tissue or organ affected by a complement-mediated disorder within the normal range.
[00101] [00101] In some modalities of an inventive method, the element (i) comprises the administration of multiple doses of a complement inhibitor to the subject according to a dosing schedule in which successive doses are administered on average by at least 2 weeks after the plasma concentration of the complement inhibitor decreases to no more than 10%, or in some modalities no more than 5%, or in some modalities no more than 1% of the maximum plasma concentration that was achieved after the previous dose. In some embodiments of an inventive method, element (i) comprises the administration of multiple doses of a complement inhibitor to the subject according to a dosing schedule in which successive doses are administered on average at least 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, or 15 weeks after the plasma complement inhibitor concentration decreases to no more than 20% of the maximum plasma concentration that was achieved after the previous dose, or in some modalities at least 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, or 15 weeks after the plasma concentration of the complement inhibitor decreases to no more than more than 10%, or in some modalities not more than 5%, or in some modalities, no more than 1% of the maximum plasma concentration that was reached after the previous dose.
[00102] [00102] In some embodiments, an inventive method comprises administering a complement inhibitor at intervals such that the subject's plasma complement activation capacity is at least 50% of the baseline value or within the normal range for, on average at least 2 weeks between doses. In some modalities, an inventive method comprises administering a complement inhibitor at intervals such that the subject's plasma complement activation capacity is at least 50% of the baseline value for, on average, at least 2 weeks between the doses. "Baseline value" in this context refers to the subject's ability to activate complement when not affected by the administration of an agent or exposure to a stimulus that significantly affects the complement system; and not having experienced an exacerbation of asthma or COPD (or, in some aspects of the invention, another complement-mediated disorder, as applicable) in the previous 6 weeks. In some modalities, an inventive dosing regimen comprises administering a complement inhibitor at intervals such that the subject's plasma complement activation capacity is within the normal range for, on average, at least 2 weeks between doses. "Normal range" in this context usually refers to a range within ± 2 standard deviations from the mean value (for example, an arithmetic mean value) in a population of individuals. A person skilled in the art will appreciate that the specific values for a "normal range" may, at least in part, depend on the specific assay used to assess the ability to activate the complement and / or factors such as the specific reagents used. In some embodiments, a normal range can be determined using published data. In some modalities, a normal range can be adequately defined by a laboratory, test center, person skilled in the art, etc.
[00103] [00103] In some embodiments, the complement inhibitor is administered with a dosage interval, such that the subject's complement activation capacity is at least 50% of the base value or within the normal range for an average of at least 3 weeks, for example, between 3 and 15 weeks, for example, between 3 and 12 weeks, for example, between 3 and 10 weeks, for example, between 4 and 8 weeks, for example, approximately 4, 5, 6, 7 or 8 weeks between doses. For the purposes of the present invention, it will be assumed that the plasma and serum complement activation capabilities are not significantly different and can be used interchangeably if there is no evidence to the contrary. If it is determined that the difference exists, the invention provides modalities in which the plasma complement activation capacity is used, modalities in which the serum complement activation capacity is used and modalities in which a value is used. medium.
[00104] [00104] In some embodiments, an inventive method comprises the administration of a complement at intervals at least equal to an average of twice (2X) the plasma half-life of the complement inhibitor when administered intravenously. In some embodiments, an inventive dosage regimen comprises the administration of a complement inhibitor at intervals at least equal to 3X, 4X, 5X, 6X, 7X, 8X, 9X, or 10X the plasma half-life of the inhibitor. complement when administered intravenously. In some modalities, an inventive method comprises
[00105] [00105] It will be appreciated that a variety of approaches can be used to determine pharmacokinetic parameters (PK) as a half-life. A suitable method can be selected by an individual skilled in the art. In general, the half-life can be determined by a method comprising: administering one or more doses of the compound to subjects, obtaining blood samples from the subject at various times after administration, measuring the concentration of the compound in the samples and calculate the half-life, at least in part based on such measurements. For example, in some modalities, samples can be obtained at time 0 (pre-dose), 5 min, 15 min, 30 min, 1 h, 4 h, 8 h, 24 hours (1 day), 48 hours (2 days), 96 h (4 days), 192 h (8 days), 14 days, 21, 28 days post-dose. It will be appreciated that these time points are exemplary. Different time points and / or more or less time points could be used in various modalities. An individual skilled in the technique would readily select appropriate time points. Blood samples are normally processed to obtain plasma or serum before taking measurements. For the purposes of the present invention, it will be assumed that the plasma and serum concentrations (and pharmacokinetic parameters, such as half-life) are not significantly different and can be used interchangeably if there is no evidence to the contrary. If it is determined that the difference exists, the invention provides modalities in which plasma concentrations (and / or plasma half-life) are used, modalities in which serum concentrations (and / or serum half-life) are used and modalities in which an average value is used.
[00106] [00106] An individual skilled in the technique must select an appropriate method to measure the compound. For example, in some modes, an immunoassay is used. In some embodiments, a method based on chromatography (for example, liquid chromatography (LC), liquid chromatography coupled with mass spectrometry (LC – MS) or liquid chromatography associated with tandem mass spectrometry (LC– MS – MS) In some modalities, a bioassay is used In many modalities, the half-life is a terminal half-life (elimination) In some modalities, a terminal half-life is calculated after administration of a In some modalities, a terminal half-life is calculated after the administration of multiple doses and allowing the concentration to reach the stationary state. In some modalities, a determined half-life for the initial phase (distribution) is used. For example, if most of the compound is removed from circulation during the distribution phase, an initial half-life can be used in some modalities.
[00107] [00107] In some modalities, half-life is determined by performing a PK analysis using non-comparative analysis
[00108] [00108] In some modalities, the half-life is determined using an appropriate dose to treat a complement-mediated chronic disorder, for example, a chronic respiratory disorder, for example, asthma or COPD. In some modalities, one dose is sufficient to reduce the plasma complement's activation capacity to no more than 50% of the lower limit of the normal range. In some embodiments, one dose is sufficient to reduce the plasma complement activation capacity to no more than twice normal levels, for example, to approximately normal levels. In some embodiments, half-life is determined using a composition comprising the complement inhibitor, where the composition is the same or similar to a composition to be used to treat a chronic complement-mediated disorder.
[00109] [00109] In certain modalities, a complement inhibitor is modified by the conjugation with a polypeptide or non-polypeptide component used to stabilize the compound, reduce its immunogenicity, increase its lifespan, increase or decrease
[00110] [00110] A variety of software tools are available to facilitate the calculation of PK parameters. For example, Phoenix NMLE or Phoenix WinNonlin software (PharSight Corp, St. Louis, MO) or Kinetica (Thermo Scientific) can be used. It will be appreciated that a reasonable half-life estimate based on a model can be used. In some embodiments, a half-life determined in a Phase I, II, or III clinical trial of a given compound and / or submitted in an application to a regulatory agency such as the FDA (for example, an IND or NDA) is used as a half-life in determining an inventive dosage interval.
[00111] [00111] In some embodiments, a method comprises administering at least 5, 10, 15, 20 or 25 doses to a subject according to an inventive dosing schedule (that is, using a dosage interval according to with the invention). In some modalities, treatment is continued for a period of at least 3, 6, 9, 12 months or more, for example, 1-2 years, 2-5 years, 5-10 years or more, for example, indefinitely.
[00112] [00112] It will be appreciated that minor deviations, such as the occasional use of a shorter or longer dosage interval compared to a dosage interval or interval specified in this document (for example, up to about 5%, 10% or 20% of doses, for example, within a time span such as 6 months, 1 year, etc.) would fall within the scope of the invention. In some embodiments, a dosage range for a subject may vary over time, or may be selected at least in part based on a measurement.
[00113] [00113] In some modalities of any of the inventive methods, a complement inhibitor is administered intravenously. In some modalities of any of the inventive methods, a complement inhibitor is administered via the respiratory route. In some embodiments of any of the inventive methods, a complement inhibitor is administered subcutaneously. In some embodiments of any of the inventive methods, a complement inhibitor is administered intramuscularly. In some embodiments of any of the inventive methods, a complement inhibitor is administered orally.
[00114] [00114] In some embodiments, a complement inhibitor is administered in a formulation that provides sustained release (also referred to as "extended release" or "controlled release") of the complement inhibitor. In some modalities in which a sustained release formulation is used, the time interval between doses is calculated based, at least in part, on the time during which the sustained release formulation releases the complement inhibitor. For example, if a sustained-release formulation releases a complement inhibitor for N weeks after administration before it becomes depleted, the invention provides a method of treating a subject comprising administering multiple doses of said sustained-release formulation to according to an administration schedule in which successive doses are administered with an average dosage interval of at least N + 3 weeks, for example, between N + 3 and N + 15 weeks, for example, between N + 3 and N + 12 weeks, for example, between N + 3 and N + 10 weeks, for example, between N + 4 and N + 8 weeks, for example, approximately every N + 4, N + 5, N + 6, N + 7 or N + 8 weeks. And bad-
[00115] [00115] All combinations of the various complement inhibitors, characteristics of the complement inhibitor (for example, class of compounds, molecular weight, half-life, molecular target, etc.) and dosage parameters (for example, the dosage interval, route of administration, etc.) and diseases, for example, respiratory diseases disclosed in this document are contemplated in various modalities of the invention. For example, in some embodiments, an inventive method comprises intravenous administration of a complement inhibitor with an average dosage interval of at least 3 weeks, for example, between 3 and 15 weeks, for example, between 3 and 12 weeks. , for example, between 3 and 10 weeks, for example, between 4 and 8 weeks, for example, about every 4, 5, 6, 7, or 8 weeks.
[00116] [00116] Also provided are methods of selecting a dosage range for administration of a complement inhibitor. In some embodiments, a method of selecting a dosage interval for administering a complement inhibitor comprises (a) obtaining a complement inhibitor half-life; and (b) selecting a dosage interval of at least 2-10 weeks longer than the half-life. In some embodiments, a method of selecting a dosage interval for administering a complement inhibitor comprises (a) obtaining a complement inhibitor half-life; and (b) selecting a dosage interval at least 3 times longer than the half-life. In some modalities, a method of selecting a dosage range for a complement inhibitor comprises: (a) determining the time during which the complement inhibitor reduces the plasma complement activation capacity by at least 50 % of the base value and / or the time during which the complement inhibitor reduces the plasma complement's activation capacity to below the normal range; and (b) selecting any of the inventive dosage ranges defined above on the basis of such measured time. In some embodiments, a method of selecting a dosage range may be further understood by testing a complement inhibitor administered according to an inventive dosing schedule for an animal that serves as a model for a complement-mediated chronic disorder, for example. example, a complement-mediated chronic respiratory disorder.
[00117] [00117] In some modalities, an inventive treatment method consists of an induction phase and a maintenance phase. In many modalities, the induction phase (if used) occurs when a subject initiates therapy. The induction phase can consist of a period of time during which a complement inhibitor is administered at a higher dose and / or at more frequent intervals or using a different route of administration than during the phase maintenance. During the maintenance phase, the complement inhibitor can be administered using any of the inventive dosing schedules and / or dosage ranges described above. For example, the complement inhibitor can be administered weekly during an induction phase and on average every 4-15 weeks, for example, every 4-8 weeks, during a maintenance phase. In some embodiments, a complement inhibitor is administered once or more times a day during an induction phase. In some embodiments, a complement inhibitor is administered at least 1, 2, 3, 4, 5, 6 or 7 times weekly during an induction phase. In some modalities, an induction phase lasts up to 1, 2, 3, 4, 5, 6, 7 or 8 weeks. In some embodiments, a dose or dosing interval is adjusted during an induction phase. For example, in some modalities, the dosage interval may be increased over time and / or the dose may be decreased or increased over time during the induction phase.
[00118] [00118] As mentioned above, in some modalities, chronic respiratory disease is asthma. Information on risk factors, epidemiology, pathogenesis, diagnosis, current asthma management, etc., can be found, for example, in "Expert Panel Report 3: Guidelines for the Diagnosis and Management of Asthma". National Heart Lung and Blood Institute. 2007. http://www.nhlbi.nih.gov/guidelines/asthma/asthgdln.pdf. ("Guidelines for
[00119] [00119] Individuals with asthma may experience exacerbations, which are identified as events characterized by a change in the individual's previous status. Severe asthma exacerbations can be defined as events that require urgent action on the part of the individual and their doctor to prevent a serious outcome, such as hospitalization or death from asthma. For example, a severe asthma exacerbation may require the use of systemic corticosteroids (for example, oral corticosteroids) in a subject whose asthma is generally well controlled without OCS or may require an increase in a stable maintenance dose. Moderate asthma exacerbations can be defined as events that are problematic for the subject, and that alert to the need for a change in treatment, but which are not serious. These events are clinically identified because they are outside the normal range of the subject's daily asthma variation.
[00120] [00120] Current asthma medications are usually classified into two general classes: long-term control medications ("control medications"), such as inhaled corticosteroids (ICS), oral corticosteroids (OCS), prolon-acting bronchodilators - gada (LABAs), leukotriene modifiers (for example, leukotriene receptor antagonists or inhibitors of leukotriene synthesis, anti-IgE antibodies (omalizumab (Xolair®)), chromoline and nedocromil, which are used to achieve and maintain the control of persistent asthma and rapid relief medications, such as short-acting bronchodilators (SABA), which are used to treat acute symptoms and exacerbations. For the purposes of the present invention, these treatments may be referred to as "conventional therapy" Treatment of exacerbations may also include increasing the dose and / or the intensity of control drug therapy, for example, an OCS course can be used to regain control of asthma. Current guidelines dictate the daily administration of control medications or, in many cases, the administration of multiple doses of control medications each day to subjects with persistent asthma (with the exception of Xolair, which is administered every 2 or 4 weeks).
[00121] [00121] A subject is generally considered to have persistent asthma if the subject suffers from symptoms on average more than two times a week and / or normally uses a fast relief medication (eg SABA) more than twice per week for symptom control. "Asthma severity" can be classified based on the intensity of treatment required to control the subject's asthma once relevant comorbidities have been treated and adherence and inhalation technique have been optimized (see, for example, GINA Re- port; Taylor, DR, Eur Respir J 2008; 32: 545–554). The description of treatment intensity can be based on the drugs and doses recommended in the step-by-step treatment algorithm found in the guidelines, such as the 2007 NHLBI Guidelines, GINA Report and its predecessors and / or standard medical books.
[00122] [00122] 'Asthma control' refers to the extent to which asthma manifestations have been reduced or removed by treatment (whether pharmacological or non-pharmacological). Asthma control can be assessed based on factors such as frequency of symptoms, nocturnal symptoms, objective measures of lung function such as spirometry parameters (for example,% FEV1 of predicted, FEV1 variability, requirement for the use of SABA to control symptoms. current and future risk can be assessed and included in determining the level of asthma control In some modalities, asthma control is defined as shown in Figure 4.3 (a), 4.3 (b), or 4.3 (c ) of the NHBLI Guidelines, for individuals aged 0-4, 5-11 or 12 years of age, respectively.
[00123] [00123] In general, an individual skilled in the art can select appropriate means of determining the level of asthma severity and / or degree of control, and any classification scheme deemed reasonable by those skilled in the art can be used.
[00124] [00124] In some embodiments of the invention, a subject suffering from persistent asthma is treated with a complement inhibitor using an inventive dosage regimen. In some modalities, the subject 5 suffers from mild or moderate asthma. In some modalities, the subject suffers from severe asthma. In some modalities, a subject has asthma that is not well controlled with conventional therapy. In some modalities, a subject has asthma that, when treated with conventional therapy, requires the use of ICS in order to be well controlled. In some modalities, a subject has asthma that cannot be well controlled, despite the use of ICS. In some modalities, a subject has asthma that, when treated with conventional therapy, requires the use of OCS in order to be well controlled. In some modalities, a subject has asthma that cannot be well controlled, despite the use of conventional high-intensity therapy that includes OCS. In some embodiments of the invention, an inventive dosage regimen comprises the administration of a complement inhibitor as a control medication, wherein the complement inhibitor is administered with reduced frequency and / or less regularly, compared to control medications. standard, maintaining at least equivalent asthma control. In some modalities, an inventive dosage regimen provides better patient acceptability, compliance, and / or convenience, compared to standard regimens for conventional control drugs, while maintaining at least equivalent asthma control. In some embodiments, a subject treated with a complement inhibitor, for example, according to an inventive dosage regimen, can significantly decrease the dose (for example, at least 50%) or substantially avoid using ICS, Xolair, and / or OCS as a control medication.
[00125] [00125] In some modalities, the subject suffers from allergic asthma, which is the case of most asthmatic individuals. In some modalities, an asthmatic subject is considered to have allergic asthma if a non-allergic trigger for asthma (eg, cold, exercise) 5 is not known and / or is not identified in a standard diagnostic evaluation. In some modalities, an asthmatic subject is considered to have allergic asthma if the subject (i) reproduces asthma symptoms (or worsening asthma symptoms) reproducibly after exposure to an allergen or allergen (s) to which the subject is subject. subject is sensitive; (ii) has specific IgE for an allergen or allergen (s) to which the subject is sensitive; (iii) exhibits a positive skin prick test to an allergen or allergen (s) to which the subject is sensitive; and / or (iv) presents other symptom (s) of a characteristic (s) consistent with atopy, such as allergic rhinitis, eczema or elevated total serum IgE. It will be appreciated that a specific allergic trigger may not be identified, but may be suspect or inferred if the subject suffers worsening of symptoms in certain environments, for example.
[00126] [00126] Inhalation allergen challenge is a technique that is widely used in the assessment of allergic airway disease. Allergen inhalation leads to cross-linking of allergen-specific IgE bound to IgE receptors in, for example, mast cells and basophils. Activation of secretory pathways occurs, resulting in the release of bronchoconstriction and vascular permeability meters. Individuals with allergic asthma can develop various manifestations following the allergen challenge, for example, early asthmatic response (EAR), late asthmatic response (LAR), airway hyperreactivity (AHR) and airway eosinophilia, each of which can be detected and quantified as known in the art. For example, airway eosinophilia can be detected as an increase in eosinophils in sputum and / or BAL fluid. RAS, sometimes referred to as the immediate asthmatic response (ARI), is a response to the inhalation allergen challenge that becomes detectable right after inhalation, usually within 10 minutes (min) of inhalation, for example , as a decrease in FEV1. EAR typically reaches a maximum of 5 within 30 min and resolves within 2-3 hours (h) post-challenge. For example, a subject can be considered to have a "positive" EAR his FEV1 decreases at least 15%, for example, at least 20%, within this time window in relation to the base value of FEV1 (where "base value" "in this context it refers to conditions before the challenge, for example, conditions equivalent to the subject's habitual condition, when he is not suffering an exacerbation of asthma and is not exposed to allergic stimuli to which the subject is sensitive). The late asthmatic response (LAR) usually starts between 3 h and 8 h post-challenge and is characterized by cellular airway inflammation, increased bronchovascular permeability and mucus secretion. It is usually detected as a decrease in FEV1, which may be greater in magnitude than that associated with RAS and potentially more clinically important. For example, a subject can be considered to have a "positive" LAR if his FEV1 decreases by at least 15%, for example, by at least 20%, compared to the base value of FEV1 within the relevant time period compared to the base value of FEV1. A delayed airway response (DAR) can occur between about 26 and 32 h, peaking between about 32 and 48 h and resolving within about 56 h after the challenge (Pelikan, Z. Ann AllergyAsthma Immunol. 2010, 104 (5): 394-404).
[00127] [00127] In some modalities, chronic respiratory disorder is chronic obstructive pulmonary disorder (COPD). COPD encompasses a spectrum of conditions characterized by limited respiratory flow that is not fully reversible even with therapy and is generally progressive. Symptoms of COPD include dyspnoea (shortness of breath), decreased exercise tolerance, coughing, sputum production, wheezing and tightness in the chest. People with COPD may experience acute worsening episodes (for example, developing during course 5 of less than a week and often over 24 hours or less) of the symptoms (called COPD exacerbations) that may vary in frequency and duration and are associated with significant morbidity. They can be triggered by events such as respiratory infection, exposure to harmful particles, or they may have an unknown etiology. Smoking is the most commonly found risk factor for COPD, and other inhalation exposures can also contribute to the development and progression of the disorder. The role of genetic factors in COPD is an area of active research. A small percentage of COPD patients have a hereditary deficiency of alpha-1 antitrypsin, an important inhibitor of the circulation of serine proteases, and this deficiency can lead to a rapidly progressive form of the disorder.
[00128] [00128] Pathophysiological characteristics of COPD include narrowing and structural changes in the small airways and destruction of the lung parenchyma (especially around alveoli), most commonly due to chronic inflammation. The chronic airflow limitation seen in COPD typically involves a mixture of these factors, and their relative importance in contributing to symptoms and the airflow limitation varies from person to person. The term "emphysema" refers to the widening of the distal air spaces (alveoli) to the terminal bronchioles, with destruction of their walls. It should be noted that the term "emphysema" is generally used clinically to refer to the medical condition associated with such pathological changes. Some individuals with COPD have chronic bronchitis, which is defined in clinical terms as a cough with sputum production on most days for 3 months of a year, for 2 consecutive years. More information on risk factors, epidemiology, pathogenesis, diagnosis and current management of COPD can be found, for example, in "Global Strategy for the Diagnosis, Management, and Pre- 5 vention of Chronic Obstructive Pulmonary Disease" (updated 2009 ) available on the website of the Global Initiative on Chronic Obstructive Pulmonary Disease, Inc. (GOLD) (www.goldcopd.org), also referred to in this document as the "GOLD report, the American Thoracic Society / European Respiratory Society Guidelines ( 2004) available on the ATS website at www.thoracic.org/ clinical / copd- guidelines / resources / copddoc.pdf, referred to here as "ATC / ERS COPD Guidelines" and standard internal medicine books such as Cecil Textbook of Medicine ( 20th edition), Harrison's Principles of Internal Medicine (17th edition) and / or standard textbooks with a focus on pulmonary medicine.
[00129] [00129] In some modalities, methods disclosed in this document inhibit (interfere in, interrupt) the cycle DC-Th17-B-Ab-C-DC discussed above. For example, administration of a complement inhibitor can break the cycle by which the complement stimulates DC cells to promote the Th17 phenotype. As a result, the number and / or activity of Th17 cells decreases, which in turn reduces the amount of Th17 mediated by stimulation of B lymphocytes and production of polyclonal antibodies. In some modalities, these effects result in "redefining" the immunological microenvironment to a more normal, less pathological state. As described in example 1, evidence that supports the ability to inhibit complement to have a prolonged inhibitory effect on the production of cytokines associated with Th17 was obtained in an animal model of asthma.
[00130] [00130] In some embodiments, inhibiting the DC-Th17-B-Ab-C-DC cycle has a disturbance modifying effect. Without wishing to be bound
[00131] [00131] In some modalities, an immunological microenvironment can become progressively more polarized towards a pathological state over time, for example, in a subject who has not yet developed the symptoms of a chronic disorder or in a subject who has developed the disorder and was treated as described in this document. Such a transition can occur stochastically (for example, due at least in part to apparently random fluctuations in antibody levels and / or affinity) and / or as a result of accumulated "sub-threshold" trigger events that are not intensively enough to cause a symptomatic outbreak of a disorder.
[00132] [00132] In some aspects, the methods disclosed here include monitoring the subject to detect evidence of a cycle DC-Th17-B-Ab-C-DC. If such evidence is detected, the subject can be treated with a complement inhibitor and / or another agent that interrupts the cycle of DC-Th17-B-Ab-C-DC. In some modalities, a subject is tested for the detection of Th17 cells (for example, the number of Th17 cells or relative number) and / or for the detection of one or more biomarkers associated with Th17 cells and / or Th17 activity ("Th17 biomarker"). In some modalities, a subject is treated with a complement inhibitor based, at least in part, on the evaluation of Th17 cells with a Th17 biomarker. "Th17 biomarker" encompasses any detectable molecule or indicator that correlates with the presence of Th17 cells (e.g., number or concentration of Th17 cells) and / or correlates with at least one Th17 cell activity. In some modalities, a Th17 biomarker comprises a level 5 of a Th17-associated cytokine. In some embodiments, a Th17-associated cytokine is a cytokine that promotes the formation and / or activation of Th17 cells, for example, IL-6, IL-21, IL-23, and / or IL-1. In some embodiments, a Th17-associated cytokine is a cytokine produced by Th17 cells, for example, IL-17 (for example, IL-17A or IL-17F), IL-21, and / or IL-22. In some modalities, an increase in the amount or an increase in the relative amount of an activity associated with Th17 is indicative of an increase in Th17 cells and / or an increase in activity associated with Th17. In some modalities, a relative amount is an amount compared to a different cytokine. In some embodiments, the different cytokine is associated with Treg cells. In some embodiments, the different cytokine is IL-10. In some modalities, levels of 2, 3, 4, 5 or more cytokines associated with Th17 are measured. A collective index or score indicating the level of activity associated with Th17 can be obtained and used as a biomarker for Th17. In some modalities, the presence or level of Th17 cells is assessed for any purpose for which a Th17 biomarker can be assessed. In some modalities, the presence or level of Tregs is assessed. In some modalities, Tregs are identified based on the expression of FOXP3.
[00133] [00133] In some modalities, a Th17 biomarker level is measured in a sample obtained from a subject. In some modalities, a sample comprises a body fluid, for example, blood, BAL fluid, sputum, nasal discharge, urine, etc. In some modalities, a sample comprises a tissue sample
[00134] [00134] In some embodiments, in vivo evaluation of Th17 cells and / or a Th17 biomarker is envisaged. For example, in some embodiments, a detectably labeled agent that binds to Th17 cells (for example, to a cell surface marker or a combination thereof that is reasonably specific to Th17 cells) or that binds to a Th17-associated cytokine is administered to a subject. An appropriate imaging method is used to view the agent in vivo. For example, in some modalities, an image of the lungs, skin or other location that can be affected by a complement-mediated disorder is obtained. In some modalities, in vivo detection allows the assessment of the immunological microenvironment in a tissue or organ of interest. In some modalities, a detectable label is made up of a fluorescent, radioactive portion, detectable by ultrasound or magnetically. In some modalities, an imaging method comprises imaging by magnetic resonance, ultrasonography, optical imaging (for example, fluorescence imaging or bioluminescence imaging) or imaging nuclear. In some embodiments, a fluorescent portion comprises a fluorescent portion of the near or infrared (emitting in the region of the near or near infrared spectrum). In some modalities, an imaging method consists of positron emission (PET), and single photon emission computed tomography (SPECT). In some embodiments, a detectable label is attached to an agent that binds directly to a target to be detected. In some embodiments, a detectable label is associated with or incorporated into or comprises particles, which in some embodiments have an agent on their surface that binds directly to a target to be detected.
[00135] [00135] In some modalities, information obtained from a Th17 biomarker assessment is used together with additional information, for example, genotype information, environmental exposure information and / or subject history information, for determine whether or when to administer a complement inhibitor and / or anti-Th17 agent and / or select a dose or dosage regimen for a subject. In some modalities, any of the biomarker assessment and / or treatment decision methods can be performed at least in part by one or more computers. In some ways, any of the biomarker assessment methods and / or treatment decisions can be incorporated or stored, at least in part, on a computer-readable medium with computer-executable instructions on it. In some modalities, a computer-readable media comprises non-transitory and / or tangible computer-readable media.
[00136] [00136] In some modalities, retreatment can occur in a fixed time scheme.
[00137] [00137] Wherever an aspect or modality of this document is described in relation to complement-mediated diseases, analogous aspects and modalities related to diseases associated with Th17 are provided. Wherever an aspect or modality of this document is described in relation to complement-mediated diseases, analogous aspects and modalities related to diseases associated with Th17 are provided. All combinations of the various complement inhibitors, characteristics of the complement inhibitor (eg class of compounds, molecular weight, half-life, molecular target, etc.) anti-Th17 agents and dosing parameters (eg , the dosage interval, route of administration, etc.) and diseases disclosed in this document are covered in several modalities. All combinations of the various complement inhibitors, characteristics of the complement inhibitor (for example, class of compounds, molecular weight, half-life, molecular target, etc.), anti-Th17 agents, characteristics of anti-Th17 agent (for example , class of compounds, molecular weight, half-life, molecular target, etc.) and
[00138] [00138] In some respects, the invention provides a method of treating a chronic complement-mediated disorder or a Th17 associated disorder comprising administering a complement inhibitor and an anti-Th17 agent to a subject who needs these. In some embodiments, the complement inhibitor and / or anti-Th17 agent is administered according to any appropriate dosage regimen. In some embodiments, the complement inhibitor and / or anti-Th17 agent is administered according to any dosage regimen described herein. In some modalities, the chronic disorder is any disorder mediated by the complement or any disorder associated with Th17. In some aspects, the invention provides methods of treating a complement-mediated chronic disorder comprising administering an anti-Th17 agent to a subject who needs it. In some modalities, the anti-Th17 agent is administered according to any appropriate dosing regimen. In some embodiments, the anti-Th17 agent is administered according to any dosage regimen described herein. In some embodiments, compositions, for example, pharmaceutical compositions, comprising a complement inhibitor and an anti-Th17 agent are provided. Exemplary anti-Th17 agents are discussed in Section V. III. Complement System
[00139] [00139] To facilitate the understanding of the invention and without the intention to limit the invention in any way, this section provides an overview of the complement and its activation pathways. More details are found, for example, in Kuby Immunology, 6th ed., 2006; Paul, W.E., Fundamental Immunology, Lippincott Williams &Wilkins; 6th ed.,
[00140] [00140] Complement is an arm of the innate immune system that plays an important role in the defense of the organism against infectious agents. The complement system comprises more than 30 whey and cellular proteins that are involved in three main pathways, known as the classic, alternative and lectin pathways. The classical pathway is usually triggered by the binding of an antigen complex and the IgM or IgG antibody to C1 (although certain other activators can also initiate the pathway). Activated C1 cleaves C4 and C2 to produce C4a and C4b, in addition to C2a and C2b. C4b and C2a combine to form C3 convertase, which cleaves C3 to form C3a and C3b. The binding of C3b to C3 convertase produces C5 convertase, which cleaves C5 to C5a and C5b. C3a, C4a and C5a are anaphylotoxins and mediate various reactions in the acute inflammatory response. C3a and C5a are also chemotactic factors that attract cells of the immune system such as neutrophils.
[00141] [00141] The alternative pathway is initiated by and amplified on, for example, microbial surfaces and several complex polysaccharides. In this way, hydrolysis from C3 to C3 (H2O), which occurs spontaneously at a lower level, leads to the binding of factor B, which is cleaved by factor D, generating a fluid phase C3 convertase that activates the complement by cleavage of C3 in C3a and C3b. C3b binds to target like cell surfaces and form a complex with factor B, which is later cleaved by factor D, resulting in a C3 convertase. Surface-bound C3 convertases cleave and activate additional C3 molecules, resulting in rapid C3b deposition in close proximity to the activation site and leading to the formation of additional C3 deconvertase, which in turn generates additional C3b. This process results in a C3 cleavage cycle and C3 convertase formation that amplifies
[00142] [00142] The C5 convertases produced in both pathways cleave C5 to produce C5a and C5b. C5b then binds to C6, C7 and C8 to form C5b-8, which catalyzes the polymerization of C9 to form the membrane attack complex (MAC) C5b-9. MAC is inserted into the membranes of target cells and causes cell lysis. Small amounts of MAC in the cell membrane can have a variety of consequences other than cell death.
[00143] [00143] The lectin complement pathway is initiated by the binding of mannose-binding lectin (MBL) and MBL-associated serine protease (MASP) to carbohydrates. The MB1-1 gene (known as LMAN-1 in humans) encodes an integral type I membrane protein, located in the intermediate region between the Golgi complex and the endoplasmic reticulum. The MBL-2 gene encodes the soluble mannose-binding protein found in serum. In the human lectin pathway, MASP-1 and MASP-2 are involved in the proteolysis of C4 and C2, leading to a C3 convertase described above.
[00144] [00144] Complement activity is regulated by various mammalian proteins, known as complement control proteins (CCP) or complement activation regulatory proteins (RCA) (Pat. No. 6,897,290). These proteins differ with respect to the specificity of the ligand and complement inhibition mechanism (s). They can accelerate the normal deterioration of convertases and / or function as cofactors for factor I, to enzymatically cleave C3b and / or C4b into smaller fragments. CCPs are characterized by the presence of multiple (typically 4-56) homologous motifs known as short consensus repetitions (SCR), complement control protein modules (CCP), or SUSHI domains, approximately 50 -70 length amino acids that contain a conserved motif, including four disulfide-linked cysteines (two disulfide bonds), proline, tryptophan and many hydrophobic residues. The CCP family includes the type 1 complement receptor (CR1; C3b: C4b receptor), complements the type 2 receptor (CR2), membrane cofactor protein (MCP; CD46), decay accelerating factor (DAF ), complement factor H (fH) and C4b binding protein (C4bp). CD59 is a membrane-regulating complement protein not structurally related to CCPs. Complement regulatory proteins usually serve to limit the activation of the complement that might otherwise occur in mammalian cells and tissues, for example, the human host. IV. Complement Inhibitors General provisions
[00145] [00145] A variety of different complement inhibitors can be used in various embodiments of the invention. In general, a complement inhibitor can belong to any of a number of classes of compounds such as peptides, polypeptides, antibodies, small molecules and nucleic acids (for example, aptamers, RNAi agents such as short interfering RNAs). In certain embodiments, a complement inhibitor inhibits an enzyme activity of a complement protein. Enzymatic activity can be proteolytic activity, such as the ability to cleave another protein
[00146] [00146] In some embodiments, a complement inhibitor comprises an antibody that substantially lacks the ability to activate the complement. For example, the antibody may have less than 10%, less than 5%, or less than 1% complement-stimulating activity compared to full-length human IgG1. In some embodiments, the antibody comprises a CH2 domain that has reduced the ability to bind to C1q compared to the CH2 domain of human IgG1. In some embodiments, the antibody contains human IgG4 CH1, CH2 and / or CH3 domains and / or does not contain human IgG1 CH1, CH2 and / or CH3 domains.
[00147] [00147] In some embodiments, a complement inhibitor used in, for example, an inventive dosage regimen, has a molecular weight of 1 kD or less. In some embodiments, a complement inhibitor has a molecular weight between 1 kD and 2 kD, between 2 kD and 5 kD, between 5 kD and 10 kD, between 10 kD and 20 kD, between 20 kD and 30 kD, between 30 kD and 50 kD, between 50 kD and 100 kD, or between 100 kD and 200 kD. [00148] A complement inhibitor can be, at least in part, identical to a natural complement inhibiting agent or a variant or fragment thereof. A variety of different complement inhibitor polypeptides are produced by viruses (for example, poxviruses, herpesviruses), bacteria (for example, Staphylococcus) and other microorganisms. Complement inhibitory proteins are produced by several parasites, for example, ectoparasites, such as ticks. A complement inhibitor may comprise at least part of a mammalian complement control or complement or receptor regulatory protein. See Ricklin, D., et al. "Complement-targeted Therapeutics", Nature Biotechnology, 25 (11): 1265-75, 2007, for discussion of complement inhibitors that are or have been in preclinical or clinical development for various diseases and can be used in various modalities of the inventive methods.
[00149] [00149] In some embodiments, a complement inhibitor consists of an adnectin, affibody, anticalin or other type of polypeptide sometimes used in the art instead of an antibody, in which the polypeptide binds to a component of the complement.
[00150] [00150] The following sections discuss exemplary non-limiting complement inhibitors for use in the modalities of the present invention. Complement inhibitors have been classified into several groups for convenience. It will be understood that certain complement inhibitors fall into several categories.
[00151] [00151] In some embodiments, a complement inhibitor that binds to substantially the same binding site (for example, a binding site on a complement component such as C3, C5, factor B, factor D, or a product complement complement) as a complement inhibitor described herein is used. In general, the ability of the first and second agents to bind substantially 5 to the same site on a target molecule, such as a complement or receptor component, can be assessed using methods known in the art, such as competition, molecular modeling, etc. (See, for example, discussion of compstatin analogue mimetics.) Optionally, the first and / or second agent can be labeled with a detectable label, for example, a radioactive label, fluorescent label, etc. Optionally, the target molecule, first agent or second agent is immobilized on a support, for example, a slide, filter, chip, spheres, etc. In some embodiments, a second antibody that binds to substantially the same binding site as a first antibody is composed of one or more CDR (s) that are at least 90%, 91%, 92%, 93% , 94%, 95%, 96%, 97%, 98%, 99% or 100% identical to the CDR (s) of the first antibody. Compounds that inhibit C3 Mimetic Activation or Activity and Compstatin Analogs
[00152] [00152] Compstatin is a cyclic peptide that binds to C3 and inhibits complement activation, for example, inhibiting C3 cleavage of C3a and C3b by convertase. Pat. US No. 6,319,897 describes a peptide having the sequence Ile- [Cys-Val-Val-Gln-Asp-Trp-Gly-His-His-Arg-Cys] -Thr (SEQ ID NO: 1), with the disulfide bond between the two cysteines denoted by brackets. It will be understood that the name "compsatin" was not used in Pat. No. 6,319,897, but was later adopted in the scientific and patent literature (see, for example, Morikis, et al., Protein Sci., 7 (3): 619-27, 1998) to refer to a peptide , to refer to a peptide with the same sequence as SEQ ID NO: 2 disclosed in U.S. Pat. US No. 6,319,897, but amidated at terminal C
[00153] [00153] Compstatin analogs can be acetylated or starched, for example, at the N-terminal and / or C-terminal. For example, compstatin analogs can be acetylated at the N-terminal and amidated at the C-terminal. Consistent with the use in the technique, "compstatin" as used in this document and the activities of compstatin analogs described here in relation to those of compstatin, refer to amidated compstatin at the C-terminal (Mallik, 2005, supra).
[00154] [00154] Compatatin concatamers or multimers or a corresponding complement inhibitor analog are also useful in the present invention.
[00155] [00155] As used herein, the term "compstatin analogue" includes compstatin and any complement inhibitor analogue thereof. The term "compstatin analogue" encompasses compstatin and other compounds designed or identified on the basis of compstatin and whose complement inhibitory activity is at least 50% as great as that of compstatin as measured, for example, using any activation of the complement accepted in the technique or substantially similar or equivalent tests. Certain suitable tests are described in Pat. No. 6,319,897, WO2004 / 026328, Mo-
[00156] [00156] The activity of a compstatin analogue can be expressed through its IC50 (the concentration of the compound that inhibits complement activation by 50%), with a lower IC50 indicating greater activity, as recognized in the art. The activity of a preferred compstatin analogue for use in the present invention is at least as great as that of compstatin. It is noted that certain modifications known to reduce or eliminate complement the inhibiting activity and can be explicitly excluded from any modality of the invention. The IC50 of compstatin was measured as 12 µM using an alternative mediated erythrocyte lysis assay (WO2004 / 026328). It will be appreciated that the exact IC50 value measured for a given compstatin analogue will vary with experimental conditions (for example, the serum concentration used in the assay). Comparative values, for example, obtained from experiments in which IC50 is determined by several different compounds under substantially identical conditions, are useful. In one embodiment, the IC50 of the compstatin analog is no more than the IC50 of compstatin. In certain embodiments of the invention, the activity of the compstatin analogue is between 2 and 99 times that of compstatin (that is, the analogue has a lower IC50 than the IC50 of compstatin by a factor between 2 and 99). For example, the activity can be between 10 and 50 times as great as that of compstatin, or between 50 and 99 times as great as that of compstatin. In certain modalities
[00157] [00157] Kd of compstatin binding to C3 can be measured using isothermal titration calorimetry (Katragadda, et al., J. Biol. Chem., 279 (53), 54987-54995, 2004). The binding affinity of a variety of compstatin analogs for C3 was correlated with their activity, with a lower Kd, indicating a higher binding affinity, as recognized in the art. A linear correlation between binding affinity and activity was shown for certain tested analogues (Katragadda, 2004, supra; Katragadda 2006, supra). In certain embodiments of the invention, the compstatin analog binds to C3 with a Kd between 0.1 µM and 1.0 µM, between 0.05 µM and 0.1 µM, between 0.025 µM and 0.05 µM, between 0.015 µM and 0.025 µM, between 0.01 µM and 0.015 µM or between 0.001 µM and 0.01 µM.
[00158] [00158] Compounds "designed or identified based on compstatin" include, among others, compounds that make up a
[00159] [00159] In certain embodiments of the invention, the compstatin analog sequence comprises or essentially consists of a sequence that is obtained by making 1, 2, 3 or 4 substitutions in the compstatin sequence, i.e., 1, 2, 3 or 4 amino acids in the sequence of compstatin they are replaced by a different standard amino acid or by a non-standard amino acid. In certain embodiments of the invention, the amino acid at position 4 is changed. In certain embodiments of the invention, the amino acid at position 9 is changed. In certain embodiments of the invention, the amino acids at positions 4 and 9 are changed. In certain embodiments of the invention, only the amino acids at positions 4 and 9 are changed. In certain embodiments of the invention, the amino acid at position 4 or 9 is altered, or in certain embodiments, both amino acids 4 and 9 are altered, and furthermore, up to 2 amino acids located at selected positions of 1, 7, 10, 11 and 13 are changed. In certain embodiments of the invention, the amino acids at positions 4, 7 and 9 are changed. In certain embodiments of the invention, the amino acids at position 2, 12, or both are changed, provided that the change preserves the ability of the compound to be cyclized. Such change (s) in positions 2 and / or 12 can be additional to the change (s) in position 1, 4, 7, 9, 10, 11 and / or 13. Optionally , the sequence of any of the compstatin analogs whose sequence is obtained by replacing one or more amino acids in the compstatin sequence further includes up to 1, 2 or 3 additional amino acids at the C-terminus. In one embodiment, the additional amino acid is Gly. Optionally, the sequence of any of the compostatin analogs whose sequence is obtained by substituting one or more amino acids from the compstatin sequence further includes up to 5, or up to 10 additional amino acids at the C-terminus. It should be understood that compstatin analogs may have one or more of the characteristics or traits of the various modalities described herein, and characteristics or traits of any modality may additionally characterize any other modality described in this document, unless otherwise indicated or evidenced otherwise by context. In certain embodiments of the invention, the sequence of the compstatin analog comprises or essentially consists of a sequence identical to that of compstatin, except in the positions corresponding to positions 4 and 9 in the sequence of compstatin.
[00160] [00160] Compstatin and certain compstatin analogs with slightly higher activity than compstatin contain only standard amino acids ("standard amino acids" are glycine, leucine, isoleucine, valine, alanine, phenylalanine, tyrosine, tryptophan, aspartic acid , asparagus, glutamic acid, glutamine, cysteine, methionine, arginine, lysine, proline, serine, threonine and histidine). Certain compstatin analogs with enhanced activity incorporate one or more non-standard amino acids. Useful non-standard amino acids include halo amino acids
[00161] [00161] In certain embodiments of the invention, the compstatin analogue comprises one or more Trp analogs (for example, at position 4 and / or 7 with respect to the compstatin sequence). Exemplary Trp analogues are mentioned above. See also Beene, et. al. Biochemistry 41: 10262-10269, 2002 (describing, among others, halogenated Trp analogs once or multiple times); Babitzke & Yanofsky, J. Biol. Chem. 270: 12452-12456, 1995 (describing, among others, methylated and halogenated Trp and other analogs of Trp and indole); and US Patents 6,214,790, 6,169,057, 5,776,970, 4,870,097, 4,576,750 and
[00162] [00162] In certain modalities, the Trp analogue increased the hydrophobic character in relation to Trp. For example, the indole ring can be replaced by one or more alkyl groups (for example, methyl). In certain modalities, the Trp analogue participates in a hydrophobic interaction with C3. Such a Trp analogue can be located, for example, at position 4 with respect to the compstatin sequence. In certain embodiments, the Trp analogue comprises a substituted or unsubstituted bicyclic aromatic ring component or two substituted or unsubstituted monocyclic aromatic ring components.
[00163] [00163] In certain modalities, the Trp analogue increased propensity to the formation of hydrogen bonds with C3 in relation to Trp, but it does not have a greater hydrophobic character in relation to Trp. The Trp analogue may have a greater polarity in relation to Trp and / or more ability to participate in an electrostatic interaction with a hydrogen bonding donor in C3. Certain eXemplar Trp analogs with a higher hydrogen bond formation feature comprise an electronegative substituent on the indole ring. Such a Trp analogue can be located, for example, at position 7, in relation to the compstatin sequence.
[00164] [00164] In certain embodiments of the invention, the compstatin analogue comprises one or more Ala analogs (for example, at position 9 in relation to the compstatin sequence), for example, Ala analogs that are identical to Ala, except that they include one or more side chain CH2 groups. In certain embodiments, the Ala analogue is a single methyl branched amino acid, such as 2-Abu. In certain embodiments of the invention, the compstatin analogue comprises one or more Trp analogs (for example, at position 4 and / or 7 with respect to the compstatin sequence) and an Ala analogue (for example, at position 9 in the compstatin sequence).
[00165] [00165] In certain embodiments of the invention, the compstatin analogue is a compound comprising a peptide that has a sequence of (X'aa) n- Gln - Asp - Xaa - Gly- (X "aa) m, (SEQ ID NO: 2) where each X'aa and each X "aa is an independently selected amino acid or amino acid analog, where Xaa is Trp or a Trp analogue, and where n> 1 in> 1 and n + m is between 5 and 21. The peptide has a core sequence of Gln - Asp-Xaa - Gly, where Xaa is Trp or a Trp analogue, for example, a Trp analogue, having increased the propensity for formation of hydrogen bonds with an H bond donor in relation to Trp, but, in certain modalities, not having a greater hydrophobic character in relation to Trp. For example, the analogue can be one in which the Trp indole ring is replaced with an electronegative portion, for example, a halogen such as fluorine. In one embodiment, Xaa is 5-fluorotryptophan. In the absence
[00166] [00166] In certain embodiments of the invention, the peptide has a core sequence of X'aa-Gln - Asp - Xaa - Gly (SEQ ID NO: 3), where X'aa and Xaa are selected from Trp and analogues of Trp. In certain embodiments of the invention, the peptide has a core sequence of X'aa-Gln - Asp - Xaa - Gly (SEQ ID NO: 3), where X'aa and Xaa are selected from Trp, analogues of Trp and other amino acids or amino acid analogs comprising at least one aromatic ring. In certain embodiments of the invention, the nucleus sequence forms a -turn in the context of the peptide. O -turn can be flexible, allowing the peptide to assume two or more conformations as assessed, for example, using nuclear magnetic resonance (NMR). In certain embodiments, X'aa is a Trp analogue comprising a substituted or unsubstituted bicyclic aromatic ring component or two or more substituted or unsubstituted monocyclic aromatic ring components. In certain embodiments of the invention, X'aa is selected from the group consisting of 2-naptylalanine, 1-naptylalanine, 2-indanylglycine carboxylic acid, dihydrotryptophan and benzoylphenylalanine. In certain embodiments of the invention, X'aa is an analog of Trp that has a greater hydrophobic character compared to Trp. For example, X'aa can be 1-methyltryptophan. In certain embodiments of the invention, Xaa is a Trp analogue that is more likely to form hydrogen bonds in relation to Trp, but, in certain embodiments, not having a greater hydrophobic character in relation to Trp. In certain embodiments of the invention, the Trp analogue that has the greatest propensity to form hydrogen bonds over Trp comprises a modification of the Trp indole ring, for example, in position 5, as a substitution from a halogen atom to an H atom at position 5. For example, Xaa can be 5-fluorotryptophan.
[00167] [00167] In certain embodiments of the invention, the peptide has a core sequence of X'aa-Gln - Asp - Xaa - Gly-X "aa (SEQ ID NO: 4), where X'aa and Xaa are independently selected at from Trp and analogs of Trp and X "aa is selected from His, Ala, analogs of Ala, Phe and Trp. In certain embodiments of the invention, X'aa is a Trp analogue that has an increased hydrophobic character over Trp, such as 1-methyltryptophan or another Trp analogue with an alkyl substituent on the indole ring (for example, at position 1 , 4, 5, or 6). In certain embodiments, X'aa is a Trp analogue that comprises a substituted or unsubstituted bicyclic aromatic ring component or two or more substituted or unsubstituted monocyclic aromatic ring components. In certain modalities of the invention, X'aa is selected from the group consisting of 2-naptylalanine, 1-naptylalanine, 2-indanylglycine carboxylic acid, dihydrotriptophan and benzoylphenylalanine. In certain embodiments of the invention, Xaa is a Trp analogue that is more likely to form hydrogen bonds with C3 than Trp, but in certain embodiments, not having a greater hydrophobic character compared to Trp. In certain embodiments of the invention, the Trp analogue that is more likely to form hydrogen bonds than Trp comprises a modification of the Trp indole ring, for example, at position 5, as a substitution for a halogen atom for an H atom at position 5. For example, Xaa can be 5-fluorotryptophan. In certain modalities, X "aa is Ala or an analogue
[00168] [00168] In certain preferred embodiments of the invention. The peptide can be cyclized through a link between any two amino acids, one of which is (X'aa) n and the other which is located within (X "aa) m. In certain embodiments, the cyclic part of the peptide is between 9 and 15 amino acids in length, for example, 10-12 amino acids in length. In certain embodiments, the cyclic part of the peptide is 11 amino acids in length, with a bond (for example, a disulfide bond) between amino acids at positions 2 and 12. For For example, the peptide can be 13 amino acids long, with a link between amino acids at positions 2 and 12, resulting in a cyclic portion with 11 amino acids long.
[00169] [00169] In certain embodiments, the peptide comprises or consists of the sequence X'aa1 - X'aa2 - X'aa3 - X'aa4 -Gln-Asp-Xaa-Gly- X "aa1- X" aa2- X "aa3- X "aa4- X" aa5 (SEQ ID NO: 5). In certain modalities, X 'aa4 and Xaa are selected from Trp and analogues of Trp, and X'aa1, X'aa2, X'aa3, X "aa1, X" aa2, X "aa3, X" aa4, and X "aa5 are independently selected from amino acids and amino acid analogues. In certain embodiments, X'aa4 and Xaa are selected from aromatic amino acids and aromatic amino acid analogues. Any one or more of one from X’aa1,
[00170] [00170] In certain embodiments, the compstatin analog is a compound that comprises a peptide having a sequence:
[00171] [00171] Xaa1 - Cys - Val - Xaa2 - Gln - Asp - Xaa2 * - Gly - Xaa3 - His - Arg - Cys - Xaa4 (SEQ ID NO: 6); wherein: Xaa1 is Ile, Val, Leu, B1-Ile, B1-Val, B1-Leu or a dipeptide comprising Gly-Ile or B1- Gly-Ile and B1 represents a first blocking portion;
[00172] [00172] Xaa2 and Xaa2 * are selected independently from Trp and Trp analogs; Xaa3 is His, Ala or an analogue of Ala, Phe, Trp or an analogue of Trp; Xaa4 is L-Thr, D-Thr, Ile, Val, Gly, a dipeptide selected from Thr-Ala and Thr-Asn or a tripeptide comprising Thr-Ala-Asn, in which a carboxyl terminal –OH of any one of the L-Thr, D-Thr, Ile, Val, Gly, Ala or Asn is optionally replaced by a second blocking portion B2; and the two Cys residues are joined by a disulfide bond. In some embodiments, Xa- a4 is Leu, Nle, His, or Phe or a dipeptide selected from Xaa5-Ala and Xaa5-Asn or a Xaa5-Ala-Asn tripeptide, where Xaa5 is selected from Leu, Nle , His or Phe and in which a carboxy-terminal of any of L-Thr, D-Thr, Ile, Val, Gly, Leu, Nle, His, Phe, Ala, or Asn is optionally substituted by a second portion of B2 block; and the two Cys residues are joined by a disulfide bond.
[00173] [00173] In other embodiments, Xaa1 is absent or is any amino acid or amino acid analog, and Xaa2, Xaa2 *, Xaa3, and Xaa4 are as defined above. If Xaa1 is absent, the Cys doo N-terminal residue may have a blocking portion B1 attached to it.
[00174] [00174] In another embodiment, Xaa4 is any amino acid or amino acid analog and Xaa1, Xaa2, Xaa2 * and Xaa3 are as defined above. In another embodiment, Xaa4 is a dipeptide selected from the group consisting of: Thr-Ala and Thr-Asn, where the carboxy-terminal -OH or Ala or Asn is optionally replaced by a second blocking B2 portion.
[00175] [00175] In any of the modalities of the compostatin analog of SEQ ID NO: 6, Xaa2 can be Trp.
[00176] [00176] In any of the modalities of the compostatin analog of SEQ ID NO: 6, Xaa2 can be a Trp analogue comprising a substituted or unsubstituted aromatic bicyclic ring component or two or more monocyclic aromatic ring components - substituted or unsubstituted co. For example, the Trp analogue can be selected from 2-naphthylalanine (2-NaI), 1-naphthylalanine (1-NaI), 2-indanylglycine (Ig1) carboxylic acid, dihydrotropithopan (Dht) and 4-benzoyl-L -phenylalanine.
[00177] [00177] In any of the modalities of the compostatin analog of SEQ ID NO: 6, Xaa2 can be a Trp analogue with increased hydrophobic character in relation to Trp. For example, the Trp analogue can be selected from 1-methyltryptophan, 4-methyltryptophan, 5-methyltryptophan and 6-methyltryptophan. In one embodiment, the analogue of
[00178] [00178] In any of the modalities of the compatin-5 analogue of SEQ ID NO: 6, Xaa2 * can be a Trp analogue such as a Trp analogue with a propensity for increased hydrogen bond formation with C3 over Trp, which, in certain modalities, did not increase the hydrophobic character in relation to Trp. In certain embodiments, the Trp analogue comprises an electronegative substituent on the indole ring. For example, the Trp analogue can be selected from 5-fluorotryptophan and 6-fluorotryptophan.
[00179] [00179] In certain embodiments of the invention, Xaa2 is Trp and Xaa2 * is an analogue of Trp that is more likely to form hydrogen bonds with C3 compared to Trp, which, in certain modalities, is not of greater character hydrophobic in relation to Trp. In certain embodiments of the compstatin analogue of SEQ ID NO: 6, Xaa2 is a Trp analogue with increased hydrophobic character over Trp, such as a Trp analogue selected from 1-methyltryptophan, 4-methyltryptophan, 5-methyltryptophan and 6-methyltryptophan, and Xaa2 * is a Trp analogue with a propensity for formation of increased hydrogen bonding with C3 in relation to Trp, which, in certain modalities, did not increase the hydrophobic character in relation to Trp. For example, in one embodiment, Xaa2 is methyltryptophan and Xa- a2 * is 5-fluorotryptophan.
[00180] [00180] In some of the modalities mentioned above, Xaa3 is Ala. In some of the above mentioned modalities, Xaa3 is a single methyl branched amino acid, for example, Abu.
[00181] [00181] The invention further provides compstatin analogs of SEQ ID NO: 6, as described above, in which Xaa2 and Xaa2 * are selected
[00182] [00182] In certain embodiments of the invention, the N- or C-terminal blocking portion of any of the compstatin analogs described herein is any group that stabilizes a peptide against degradation that would otherwise occur in the blood or mammalian interstitial fluid (for example, human or non-human primate). For example, the blocking portion B1 could be any portion that alters the N-terminal structure of a peptide in order to inhibit cleavage of a peptide bond between the N-terminal amino acid of the peptide and the adjacent amino acid. The B2 blocking portion could be any portion that alters the C-terminal structure of a peptide in order to inhibit cleavage of a peptide bond between the C-terminal amino acid of the peptide and the adjacent amino acid. Any suitable blocking portions known in the art could be used. In certain embodiments of the invention, the blocking portion B1 comprises an acyl group (i.e., the portion of a carboxylic acid that remains after removal of the -OH group). The acyl group generally comprises between 1 and 12 carbons, for example, between 1 and 6 carbons. For example, in certain embodiments of the invention, the blocking portion B1 is selected from the group consisting of: formyl, acetyl, proprionyl, butyryl, isobutyryl, valeryl, isovaleryl, etc. In one embodiment, the blocking portion B1 is an acetyl group, that is, Xaa1 is Ac-Ile, Ac-Val, Ac-Leu, or Ac-Gly-Ile.
[00183] [00183] In certain embodiments of the invention, the blocking portion B2 is a primary or secondary amine (-NH2 or -NHR1, where R is an organic portion, such as an alkyl group).
[00184] [00184] In certain embodiments of the invention, the blocking portion B1 is any group that neutralizes or reduces the positive charge that, otherwise, may be present at the N-terminus at physiological pH. In certain embodiments of the invention, the blocking portion 5 B2 is any group that neutralizes or reduces the negative charge that, otherwise, may be present at the C-terminus at physiological pH.
[00185] [00185] In certain embodiments of the invention, the compstatin analogue is acetylated or amidated from the N-terminal and / or C-terminal, respectively. A compstatin analog can be acetylated at the N-terminal, amidated at the C-terminal, and / or either acetylated at the N-terminal or amidated at the C-terminal. In certain embodiments of the invention, a compstatin analogue comprises an alkyl or aryl group at the N-terminus, instead of an acetyl group.
[00186] [00186] In certain embodiments, the compstatin analog is a compound that comprises a peptide having a sequence:
[00187] [00187] Xaa1 - Cys - Val - Xaa2 - Gln - Asp - Xaa2 * - Gly - Xaa3 - His - Arg - Cys - Xaa4 (SEQ ID NO: 7); where: Xaa1 is Ile, Val, Leu, Ac-Ile, Ac-Val, Ac-Leu or a dipeptide comprising Gly-Ile or Ac-Gly-Ile;
[00188] [00188] Xaa2 and Xaa2 * are selected independently from Trp and Trp analogs; Xaa3 is His, Ala or an analogue of Ala, Phe, Trp or an analogue of Trp; Xaa4 is L-Thr, D-Thr, Ile, Val, Gly, a dipeptide selected from Thr-Ala and Thr-Asn or a tripeptide comprising Thr-Ala-Asn, in which a carboxyl terminal –OH of any one of the L-Thr, D-Thr, Ile, Val, Gly, Ala or Asn is optionally replaced by –NH2; and the two Cys residues are joined by a disulfide bond. In some embodiments, Xaa4 is Leu, Nle, His, or Phe or a dipeptide selected from Xaa5-Ala and Xaa5-Asn or a Xaa5-Ala-Asn tripeptide, where Xaa5 is selected from Leu, Nle , His or Phe and in which a carboxy-terminal -OH of any of the L-
[00189] [00189] In some embodiments, Xaa1, Xaa2, Xaa2 *, Xaa3 and Xa- 5 a4 are as described above for the various embodiments of SEQ ID NO: 6. For example, in certain embodiments, Xaa2 * is Trp. In certain embodiments, Xaa2 is a Trp analogue with increased hydrophobic character over Trp, for example, 1-methyltryptophan. In certain embodiments, Xaa3 is Ala. In certain modalities, Xaa3 is a single methyl branched amino acid.
[00190] [00190] In certain embodiments of the invention, Xaa1 is Ile and Xaa4 is L-Thr.
[00191] [00191] In certain embodiments of the invention, Xaa1 is Ile, Xaa2 * is Trp and Xaa4 is L-Thr.
[00192] [00192] The invention further provides compstatin analogs of SEQ ID NO: 7, as described above, wherein Xaa2 and Xaa2 * are independently selected from Trp, Trp analogs and other amino acids or amino acid analogs, and Xaa3 is His, Ala or an Ala, Phe, Trp analog, a Trp analogue or other aromatic amino acid or aromatic amino acid analog.
[00193] [00193] In certain embodiments of any of the compstatin analogues described in this document, a Phe analogue is used instead of Phe.
[00194] [00194] Table 2 provides a list of non-restrictive compstatin analogs useful in the present invention. The analogues are referred to in an abbreviated form in the left column, indicating specific changes in the designated positions (1-13), in comparison with the parental peptide, compstatin. Consistent with the use in the art, "compstatin" as used in this document and the analogous activities of compstatin described here in relation to those of compstatin, refer to the amidated compstatin peptide at the C-terminus.
[00195] [00195] In certain embodiments of the compositions and methods of the invention, the compstatin analog has a sequence selected from sequences 9-36. In certain embodiments of the compositions and methods of the invention, the compstatin analogue has a sequence selected from SEQ ID NO: 14, 21, 28, 29, 32, 33, 34 and 36. In certain embodiments of the compositions and / or methods of the invention, the compstatin analogue has a sequence selected from SEQ ID NO: 30 and 31. In a fashion of the compositions and methods of the invention, the compostatin analogue has a sequence of SEQ ID NO : 28. In one embodiment of the compositions and methods of the invention, the compstatin analogue has a sequence of SEQ ID NO: 32. In one embodiment of the compositions and methods of the invention, the compstatin analog has a sequence of SEQ ID NO: 34. In one embodiment of the compositions and methods of the invention, the compstatin analogue has a sequence of SEQ ID NO: 36.
[00196] [00196] In some embodiments, a B1 blocking portion comprises an amino acid, which can be represented as Xaa0. In some embodiments, the blocking portion B2 comprises an amino acid, which can be represented as XaaN.
[00197] [00197] In some embodiments, a compstatin analogue comprises B1a-Xaa0 - Xaa1 - Cys - Val -Xaa2 -Gln - Asp - Xaa2 * -
[00199] [00199] In some embodiments, Xaa0 and / or XaaN in any compstatin analog sequence comprises an amino acid comprising an aromatic ring having an alkyl substituent in one or more positions. In some embodiments, an alkyl substituent is a lower alkyl substituent. For example, in some modalities, an alkyl substituent is a methyl or ethyl group. In some modalities, a substituent is located in any position that does not destroy the aromatic character of the compound. In some ways, a substituent is located in any position that does not destroy the aromatic character of a ring to which the substitute is connected. In some embodiments, a substituent is located at position 1, 2, 3, 4 or 5. In some embodiments, Xaa0 comprises an O-methyl tyrosine, 2-hydroxyphenylalanine or 3-hydroxyphenylalanine analogue. For the purposes of the present disclosure, a lowercase "m" followed by a three letter abbreviation of amino acid can be used to indicate specifically that the amino acid is an N-methyl amino acid. For example, where the acronym "mGly" appears in this document, it denotes N-methyl glycine (also sometimes referred to as sarcosine or Sar). In some embodiments, Xaa0 is or comprises mGly, Tyr, Phe, Arg, Trp, Thr, Tyr (Me), Cha, mPhe, mVal, mIle, mAla, DTyr, D-
[00200] [00200] In some embodiments, a blocking portion or part thereof, for example, an amino acid residue, can contribute to increasing the compound's affinity for C3 or C3b and / or improving the activity of the compound. In some embodiments, a contribution to the affinity or activity of an amino acid or amino acid analog may be more significant than a blocking activity.
[00201] [00201] In certain embodiments of the compositions and methods of the invention, the compstatin analogue has a sequence as set out in Table 2, but where the group Ac- is replaced by an alternative blocking portion B1, as described herein. In some embodiments, the group -NH2 is replaced by an alternative blocking portion B2, as described herein.
[00202] [00202] In one embodiment, the compstatin analogue binds to substantially the same region of the human C3 chain as does compstatin. In one embodiment, the compstatin analogue is a compound that binds to a fragment of the C-terminal part of the human C3 chain having a molecular weight of about 40 kDa, to which compstatin binds (Soulika, AM, et al., Mol. Immunol., 35: 160, 1998; Soulika, AM, et al., Mol. Immunol. 43 (12): 2023-9, 2006). In certain embodiments, the compstatin analogue is a compound that binds to the compstatin binding site as determined in a compstatin-C3 structure, for example, a crystalline structure or 3D structure derived from NMR. In certain embodiments, the compstatin analogue is a compound that can replace compostatin in a compstatin-C3 structure and would form substantially the same intermolecular contacts with C3 as compstatin. In certain embodiments, the compstatin analogue is a compound that binds to a peptide binding site having a sequence set out in Table 2, for example, SEQ ID NO: 14, 21, 28, 29, 32, 33, 34 or 36 or another compstatin analog sequence in a C3 peptide structure, for example, a crystalline structure. In certain embodiments, the compstatin analog is a compound that binds to a peptide binding site having SEQ ID NO: 30 or 31 in a C3 peptide structure, for example, a crystalline structure. In certain embodiments, the compstatin analogue is a compound that can replace the peptide of SEQ ID NO: 9-36, for example, a compound that can replace the peptide of SEQ ID NO: 14, 21, 28, 29, 32, 33, 34 or 36 or another compstatin analogue sequence disclosed herein in a C3 peptide structure and would form substantially the same intermolecular contacts with C3 as the peptide. In certain embodiments, the compstatin analog is a compound that can replace the peptide of SEQ ID NO: 30 or 31 in a compstatin-C3 structure and would substantially form the same intermolecular contacts with C3 as the peptide.
[00203] [00203] A person skilled in the art will readily be able to determine whether a compstatin analog binds to a fragment of the C-terminal part of the C3 chain using routine experimental methods. For example, a person skilled in the art could synthesize a photo-crosslinkable version of the compstatin analog, including a photo-crosslinking amino acid, such as p-benzoyl-L-phenylalanine (Bpa) in the compound, for example, in C- end of the sequence (Soulika, AM, et al, supra). Optionally additional amino acids, for example, an epitope tag such as a FLAG tag or an HA tag could be included to facilitate detection of the compound, for example, by Western blotting. The compstatin analog is incubated with the fragment and crosslinking is started. The colocalization of the compstatin analog and the C3 fragment indicates binding. Surface plasmon resonance can also be used to determine whether a compstatin analogue binds to the C3 binding site or a fragment thereof. A person skilled in the art would be able to use molecular modeling software to predict whether a compound would form substantially the same intermolecular contacts with C3 that compstatin would form or a peptide with the sequence of any of the peptides in Table 1, for example, SEQ ID NO: 14, 21, 28, 29, 32, 33, 34 or 36 or, in some embodiments, SEQ ID NO: 30 or 31 or other compstatin analog sequence disclosed herein.
[00204] [00204] Compstatin analogs can be prepared by various synthetic methods of peptide synthesis known in the art through the condensation of amino acid residues, for example,
[00205] [00205] The analogue of compstatin can be modified by adding a molecule such as polyethylene glycol (PEG) or similar molecules to stabilize the compound, reduce its immunogenicity, increase its lifetime in the body, increase or decrease its solubility, or increase its resistance to degradation. PEGylation methods are well known in the art (Veronese, F.M. & Harris, Adv. Drug Deliv. Rev. 54, 453-456, 2002; Davis, F.F., Adv. Drug Deliv. Rev. 54, 457-458, 2002); Hinds, K.D. & Kim, S.W. Adv. Drug Deliv.
[00206] [00206] In some embodiments, a compstatin analogue for use in the methods described in this document is a long-acting compsatin analogue, which has a terminal half-life of at least 3, 4, 5, 6 or 7 days. In modalities, a long-acting compostatin analogue is a PEGylated compstatin analogue. Exemplary long-acting compstatin analogs are described below and / or in PCT / US12 / 37648, entitled "CELL-REACTIVE, LONG-ACTING, OR TARGETED COMPSTATINA ANALOGS AND USES THEREOF", filed on May 11, 2012. On some modalities of any method or composition related to an analysis
[00208] [00208] The compstatin analog portions can be attached directly to the polymeric support or can be connected via a linker portion that connects the compstatin analog portion to the polymeric support. The binding portion can be attached to a single analogous portion of compstatin and to the polymeric support. Alternatively, a linker portion may have multiple portions of compostatin analog joined to it so that the linker portion links multiple portions.
[00209] [00209] In some embodiments, a compstatin analogue comprises an amino acid having a side chain comprising a primary or secondary amine, for example, a Lys residue. 5 For example, any of the compstatin analogue sequences disclosed in this document can be extended or modified by adding a linker comprising one or more amino acids, for example, one or more amino acids comprising a primary or secondary amine, for example, in a side chain of the same. For example, a Lys residue, or a sequence comprising a Lys residue, is added at the N-terminal and / or C-terminal of the compstatin analog. In some embodiments, the Lys residue is separated from the cyclic portion of the compstatin analog by a flexible or rigid spacer. A binder or spacer can, for example, comprise a saturated or unsaturated, substituted or unsubstituted alkyl chain, oligo (ethylene glycol) chain, and / or other moieties. The chain length can be, for example, between 2 and 20 carbon atoms. In some embodiments, the spacer is or comprises a peptide. The peptide spacer can be, for example, between 1 and 20 amino acids in length, for example, between 4 and 20 amino acids in length. Suitable spacers may comprise or consist of multiple residues of Gly, residues of Ser, or both, for example. Optionally, the amino acid that has a side chain comprising a primary or secondary amine and / or at least one amino acid in a spacer is a D-amino acid. A PEG moiety or similar molecule or polymeric support can be linked to the primary or secondary amine, optionally, via a linker. In some embodiments, a bifunctional ligand is used. A bifunctional ligand can include two reactive functional groups, which can be the same or different in several modalities.
[00210] [00210] Any of a variety of polymeric main structures or supports could be used. For example, the main polymeric structure or support can be a polyamide, polysaccharide, polyanhydride, polyacrylamide, polymethacrylate, polypeptide, polyethylene oxide, or dendrimer. Suitable polymeric main structures and methods are described, for example, in WO98 / 46270 (PCT / US98 / 07171) or WO98 / 47002 (PCT / US98 / 06963). In one mode, the polymeric backbone or support comprises multiple reactive functional groups, such as groups of carboxylic acids, anhydride, or succinimide. The polymeric main structure or support is reacted with compstatin analogs. In one embodiment, the compstatin analog comprises any one of a number of different reactive functional groups, such as groups of carboxylic acids, anhydride or succinimide, which are reacted with appropriate groups in the polymeric backbone. Alternatively, monomeric units that could be joined to one another to form a main polymeric or support structure are first reacted with the compstatin analogs and the resulting monomers are polymerized. In another embodiment, short chains are prepolymerized, functionalized, and then a mixture of short chains of different composition is assembled into longer polymers.
[00211] [00211] In some aspects, a portion such as a polyethylene glycol (PEG) chain or other polymer (s) that, for example, stabilizes the compound, increases its life span in the body, increases its solubility, decreases its immunogenicity and / or increases its resistance to degradation can be referred to here as a "release reduction moiety" (CRM), and a compstatin analogue comprising such a moiety can be referred to as a compstati-
[00212] [00212] In some respects, a long-acting compstatin analogue comprises a compound of the formula M – L – A, where A is a portion comprising a CRM, L is a linker portion optionally present, and M comprises a portion of compstatin analog. The compstatin analogue portion can comprise any compstatin analogue, for example, any compstatin analogue described above, in various embodiments. The formula M – L – A encompasses modalities in which LA is present in the N-terminus of the compstatin analog portion, modalities in which LA is present in the C-terminus of the compstatin analog portion, modalities in which LA it is linked to a side chain of an amino acid of the compstatin analogue portion, and modalities where the same or different L-As are present at both ends of M. It will be appreciated that when determined analogue (s) of compstatin is (are) present (s) as a portion of compstatin analog in a compound of formula M – L – A, a functional group of the compstatin analog will have reacted with a functional group of L to form a covalent bond for A or L. For example, a long-acting compstatin analog where the compstatin analog portion comprises a compstatin analog that contains an amino acid with a side chain containing a primary amine group (NH2) (whose analog of compstatin can be represented by the formula to R1— (NH2)), can have a formula R1 — NH — L– A in which a new covalent bond to L (for example, N — C) has been formed and a hydrogen lost. Thus, the term "compostatin analog portion" includes molecular structures in which at least one atom of a compstatin analog participates in a covalent bond with a second portion, which can, for example, make a modification of a chain side. Similar considerations apply to portions of compstatin analog present in multivalent compounds.
[00214] [00214] L can comprise one or more of any of the portions described in the previous paragraph, in various modalities. In some embodiments, L comprises two or more different portions bonded together to form a structure normally having a length of between 1 to about 60 atoms, between 1 to about 50 atoms, for example, between 1 and 40, between 1 and 30, between 1 and 20, between 1 and 10 or between 1 and 6 atoms, where length refers to the number of atoms in the main (longest) chain. In some embodiments, L comprises two or more different portions, connected to each other to form a structure normally having between 1 to about 40, for example, between 1 and 30, for example, between 1 and 20, between 1 and 10 or between 1 and 6 carbon atoms in the main (longest) chain.
[00215] [00215] In some embodiments, a long-acting compstatin analogue has an average plasma half-life of at least 1 day, for example, 1 to 3 days, 3 to 7, 7 to 14 days, or 14 to 28 days, when administered IV at a dose of 10 mg / kg to humans or non-human primates. In some embodiments, the mean plasma half-life of an extended-acting compstatin analog after IV administration at a dose of 10 mg / kg to humans or non-human primates is increased by at least a factor of 2, for example, by a factor of 2 to 5, 5 to 10, 10 to 50 or 50 to 100 times compared to that of a corresponding compstatin analogue having the same amino acid sequence (and, if applicable)
[00216] [00216] In some embodiments, a plasma half-life is a terminal half-life after administration of a single IV dose. 5 In some modalities, a plasma half-life is a terminal half-life after a steady state has been achieved after the administration of multiple IV doses. In some modalities, a long-acting compstatin analogue achieves a Cmax in plasma at least 5 times greater than that of a corresponding compstatin analogue not comprising CABG, for example, between 5- and 50 times greater , after the administration of a single IV dose to a primate, or after the administration of multiple IV doses. In some modalities, a long-acting compstatin analogue achieves a Cmax in plasma between 10 and 20 times greater than that of a corresponding compstatin analogue not comprising CABG after the administration of a single IV dose to a primate, or after the administration of multiple IV doses. In some modalities a primate is a human being. In some embodiments, a primate is a non-human primate, for example, a monkey, such as a Cynomolgus monkey or a Rhesus monkey. In some embodiments, the renal release of a long-acting compstatin analog during the first 24 hours after IV administration at a dose of 10 mg / kg to humans or non-human primates is reduced by at least a factor of 2, for example, by a factor of 2 to 5, 5 to 10, 10 to 50 or 50 to 100 times compared to the renal release of a corresponding compstatin analog. The concentration of compostatin analogue can be measured in blood and / or urine samples using, for example, UV, HPLC, mass spectrometry (MS) or antibody to the CRM, or combinations of such methods, such as LCMS or LCMSMS. Pharmacokinetic parameters, such as half-life and release
[00218] [00218] In some embodiments, a portion of (CH2CH2O) n is monodispersed and the polydispersity of a portion of (CH2CH2O) n is
[00220] [00220] In genera and compounds represented here, a portion of polyethylene glycol is removed with the oxygen atom on the right side of the repeat unit or on the left side of the repeat unit. In cases where only one orientation is withdrawn, the present invention encompasses both orientations (that is, (CH2CH2O) n and (OCH2CH2) n) portions of polyethylene glycol for a given compound or genus, or in cases where a compound or genus contains multiple portions of polyethylene glycol, all combinations of guidelines are encompassed by the present disclosure.
[00221] [00221] Formulas for some exemplary monofunctional PEGs comprising a reactive functional group are illustrated below. For 5 illustrative purposes, formulas in which the reactive functional group (s) comprise an NHS ester are represented, but other reactive functional groups could be used, for example, as described above. In some embodiments, (CH2CH2O) n is represented as a left-ended termination with a methoxy group (OCH3), but it will be understood that the chains represented below and elsewhere in this document may end with a different OR portion (for example, an aliphatic group, an alkyl group, a lower alkyl group or any other suitable PEG terminal group) or an OH group. In addition, it will be appreciated that portions other than those described can connect the portions of (CH2CH2O) n with the NHS group in various modalities.
[00222] [00222] In some embodiments, a monofunctional PEG is of formula A: where the "reactive functional group" and n are as defined above and described in classes and subclasses in this document; R1 is a hydrogen group, aliphatic, or any suitable end group; and T is a covalent bond or a straight or branched C1-12 hydrocarbon chain in which one or more carbon units of T are optionally and independently replaced by -O-, -S-, -N (Rx ) -, -C (O) -, -C (O) O -, - OC (O) -, -N (Rx) C (O) -, -C (O) N (Rx) -, - S ( O) -, -S (O) 2-, -N (Rx) SO2-, or -SO2N (Rx) -; and each Rx is independently hydrogen or C1-6 aliphatic.
[00223] [00223] Exemplary monofunctional PEGs of formula A include: Formula I
[00224] [00224] In Formula I, a portion comprising the reactive functional group has the general structure -CO- (CH2) m-COO-NHS, where m = 2. 5 In some embodiments, monofunctional PEGs have the structure of Formula I , where m is between 1 and 10, for example, between 1 and 5. For example, in some modalities m is 3, as shown below: Formula Ia.
[00225] [00225] In Formula II, a portion comprising the reactive functional group has the general structure - (CH2) m-COO-NHS, where m = 1. In some embodiments, a monofunctional PEG has the formula II structure, where m is between 1 and 10 (for example, where m is 5, as shown in Formula III below), or where m is 0 (as mos - shown below in Formula IIIa).
[00226] [00226] In some embodiments a linear bifunctional PEG comprises a portion comprising a reactive functional group at each of its ends. The reactive functional groups can be the same (homobifunctional) or different (heterobifunctional). In some embodiments, the structure of a bifunctional PEG can be symmetrical, in which the same portion is used to connect the reactive functional group to oxygen atoms at each end of the - (CH2CH2O) n chain. In some embodiments, different portions are used to connect the two reactive functional groups to the PEG portion of the molecule. The structures of the exemplary bifunctional PEGs are shown below. For illustrative purposes, formulas in which the reactive functional group (s) comprise an NHS ester are represented, but other reactive functional groups could be used.
[00227] [00227] In some modalities, a bifunctional linear PEG is of formula B: in which each T and "reactive functional group" are independently, as defined above and, described in classes and subclasses in this document, en is as defined above and described in classes and subclasses in this document.
[00228] [00228] Exemplary bifunctional PEGs of formula B include: Formula IV
[00229] [00229] In Formula IV, the portion comprising the reactive functional group has the general structure - (CH2) m-COO-NHS, where m = 1. In some modalities, bifunctional PEGs have the structure of Formula IV, where m is between 1 and 10, for example, between 1 and 5.
[00230] [00230] In Formula V, the portion comprising the reactive functional group has the general structure -CO- (CH2) m-COO-NHS, where m = 2. In some modalities, bifunctional PEGs have the structure of Formula V, where m is between 1 and 10, for example, between 1 and 5.
[00231] [00231] In some embodiments, a branched, comb-like, or star-shaped PEG comprises a portion comprising a reactive functional group at the end of each of the multiple (- CH2CH2O) n chains. The reactive functional groups can be the same or they can be at least two different groups. In some embodiments, a branched, comb-type, or star-shaped PEG is of the following formulas:
[00232] [00232] Where each R2 is independently a "reactive functional group" or R1, and each T, n, and "reactive functional group" is independently as defined above and described in classes and subclasses in this document.
[00233] [00233] The structure of exemplary branched PEGs (having 4 arms, or branches) comprising portions of NHS as reactive functional groups is described below: 5 Formula VIII Formula IX
[00234] [00234] The number of branches emanating from the main structure can be varied. For example, the number 4 in formulas VI and VII above can be changed to any other integer between 0 and 10 in various ways. In certain embodiments, one or more branches do not contain a reactive functional group and the branch ends with a -CH2CH2OH or -CH2CH2OR group, as described above.
[00235] [00235] In some modalities a branched PEG has the structure
[00236] [00236] In some embodiments a branched PEG has the structure of Formula VII, VIII, or IX (or variants of them having different numbers of branches) with the proviso that x is
[00237] [00237] Evidently the methylene group (CH2) in the x-portion above may, instead, comprise a longer alkyl chain (CH2) m, where m is up to 2, 3, 4, 5, 6, 8, 10, 20 or 30, or may comprise one or more other portions described herein.
[00238] [00238] In some embodiments, exemplary branched PEGs having NHS or maleimide reactive groups are described below: Formula X Formula XI
[00239] [00239] In some modalities, a variant of Formula X or XI is used, in which 3 or each of the four branches comprise a reactive functional group.
[00240] [00240] Still other examples of PEGs can be represented as follows: Formula XII Formula XIII
[00241] [00241] As noted above, it will be appreciated that, as described in this document, in various embodiments, any one of a variety of portions can be incorporated between the peptide component and the (CH2CH2O) nR portion of an analog of long-acting compstatin, such as a linear alkyl, ester, amide, aromatic ring (for example, a substituted or unsubstituted phenyl), a substituted or unsubstituted cycloalkyl structure, or combinations thereof. In some embodiments, such portions may make the compound more susceptible to hydrolysis, which can release the peptide portion of the CRM compound. In some embodiments, such release may increase tissue penetration and / or compound activity in vivo. In some embodiments, hydrolysis is general hydrolysis (for example, acid-base). In some embodiments, hydrolysis is catalyzed by enzyme, for example, catalyzed by esterase. Naturally, both types of hydrolysis can occur. Examples of PEGs comprising one or more of these moieties and an NHS ester as a reactive functional group are as follows: Formula XIV Formula XV Formula XVI 5 [00242] In some embodiments a branched PEG (multiple arms) or type PEG star comprises a pentaerythritol core, hexaglycerin core, or tripentaerythritol core. It will be understood that not all branches can emanate from a single point in certain modalities.
[00243] [00243] Monofunctional, bifunctional, branched, and other PEGs comprising one or more reactive functional groups can be obtained from, for example, NOF America Corp. White Plains, NY or BOC Sciences 45-16 Ramsey Road Shirley, NY 11967, USA, among others.
[00244] [00244] In some embodiments, a compstatin analogue of any of SEQ ID NOs: 3-41 is extended by one or more amino acids at the N-terminal, C-terminal, or both, where at least one of the amino acids has a side chain comprising a reactive functional group such as a primary or secondary amine, a sulfhydryl group, a carboxyl group (which may be present as a carboxylate group), a guanidino group, a phenol group, an indole ring,
[00245] [00245] In certain discussions at present, an amino acid having a side chain containing an amine group is used as an example. Analogous modalities are included in which an amino acid having a side chain containing a different reactive functional group is used. In some embodiments, an amino acid having a side chain comprising a primary or secondary amine is linked directly to the N-terminal or C-terminal of any one of SEQ ID NOs: 3-41 via a peptide bond. In some modalities, an amino acid having a side chain comprising a primary or secondary amine is attached to the N- or C-terminal of any of SEQ ID NOs: 3-41 via a linker portion, which can contain any of one or more of the binding portions described above. In some embodiments, at least two amino acids are attached to either or both ends. The two or more attached amino acids can be joined to each other by peptide bonds or at least some of the amino acids
[00246] [00246] It will be understood that a corresponding compstatin analog not comprising CRM may also lack one or more of those amino acids that are present in the long-acting compostatin analog to which it corresponds. Thus, a corresponding compstatin analogue comprising any of SEQ ID NOs: 3-41 and not having a CRM will be understood as "having the same amino acid sequence" as SEQ ID NOs: 3-41, respectively. For example, a corresponding compstatin analogue comprising the amino acid sequence of SEQ ID NO: 14, 21, 28, 29, 32, 33, 34 or 36 and not having a CRM will be understood as "having the same amino acid sequence "as SEQ ID NO: 14, 21, 28, 29, 32, 33, 34 or 36, respectively.
[00247] [00247] For descriptive purposes, a peptide having the amino acid sequence Ile-Cys * -Val- (1Me) Trp-Gln-Asp-Trp-Gly-Ala-His-Arg-Cys * - Thr (SEQ ID NO: 42 ) (corresponding to the compstatin analog of SEQ ID NO: 28, where the asterisks in SEQ ID NO: 42 represent cysteines linked by a disulfide bond in the active compound, and (1Me) Trp represents 1-methyl-tryptophan)), it is used as an exemplary compstatin analogue portion; (CH2) n and (O-CH2-CH2) n are used as an example of linking moieties; lysine is used as an example of an amino acid comprising a reactive functional group (in some compounds), and acetylation and amidation of the N- and C-terminals, respectively, are used as exemplary blocking moieties optionally present in some compounds and can be represented in italics, that is, as Ac and NH2, respectively. In some embodiments, SEQ ID NO: 42 is extended to comprise a Lys residue at the N- or C-terminal of the peptide, for example, as e-
[00248] [00248] Ac-Ile-Cys * -Val- (1Me) Trp-Gln-Asp-Trp-Gly-Ala-His-Arg- Cys * -Thr-Lys-NH2 (SEQ ID NO: 43).
[00249] [00249] In some embodiments, a Lys residue is linked to the 5 N- or C-terminal of SEQ ID NO: 42 via a peptide linker, for example, as exemplified below by a C-terminal link:
[00250] [00250] Ac-Ile-Cys * -Val- (1Me) Trp-Gln-Asp-Trp-Gly-Ala-His-Arg- Cys * -Thr- (Gly) 5-Lys-NH2 (SEQ ID NO: 44 ).
[00251] [00251] In some embodiments, a linker comprising a primary or secondary amine is added to the N- or C-terminal of a compstatin analog. In some embodiments, the linker comprises an alkyl chain and / or a portion of oligo (ethylene glycol). For example, NH2 (CH2CH2O) nCH2C (= O) OH (eg 8-amino-3,6-dioxaoctanoic acid (AEEAc) or 11-amino-3,6,9-trioxaundecanoic acid) or an NHS ester of even (for example, an NHS ester of 8-amino-3,6-dioxaoctanoic acid or 11-amino-3,6,9-trioxaundecanoic acid), can be used. In some embodiments, the resulting compound is as follows (where the portion contributed by the linker is shown in bold):
[00252] [00252] NH2 (CH2) 5C (= O) -Ile-Cys-Val- (1Me) Trp-Gln-Asp-Trp-Gly- Ala-His-Arg-Cys-Thr-NH2 (SEQ ID NO: 45) .
[00253] [00253] NH2 (CH2CH2O) 2CH2C (= O) -Ile-Cys-Val- (1Me) Trp-Gln-Asp- Trp-Gly-Ala-His-Arg-Cys-Thr-NH2 (SEQ ID NO: 46)
[00254] [00254] In some embodiments, a Lys residue is linked to the N- or C-terminal of SEQ ID NO: 42 via a linker comprising a non-peptide portion. For example, the linker may comprise an alkyl chain, oligo (ethylene glycol) chain, and / or cyclic ring system. In some embodiments, 8-AEEAc or an NHS ester of the same is used, resulting (in the case of Lys binding at the C-terminal) in the following compound (where the portion contributed by 8-AEEAc is shown in bold) :
[00255] [00255] Ac-Ile-Cys * -Val- (1Me) Trp-Gln-Asp-Trp-Gly-Ala-His-Arg- Cys * -Thr -NH-CH2CH2OCH2CH2OCH2-C (= O) -Lys-NH2 ( SEQ ID NO: 5 47)
[00256] [00256] It will be appreciated that in SEQ ID NOs: 45 and 46, a portion of -C (= O) is attached to the adjacent Ile residue via a CN bond, where N is part of the amino acid and is not shown . Similarly, in SEQ ID NO: 46, a portion of -C (= O) is attached to the adjacent Lys residue via a CN bond, where N is part of the amino acid and is not shown . It will be appreciated that in SEQ ID NO: 47 the NH portion is linked to the N-terminal amino acid (Thr) immediately, via a CN bond, where C is the carbonyl carbon of the amino acid and not is shown.
[00257] [00257] The compounds of SEQ ID NOs: 43-47 can be readily modified in the primary amine group to produce a long-acting compstatin analogue.
[00258] [00258] Exemplary long-acting compstatin analogs are set out below, where n is sufficient to provide an average molecular weight of between about 500; 1,000; 1,500; 2,000; 5,000;
[00259] [00259] (CH2CH2O) nC (= O) -Ile-Cys-Val- (1Me) Trp-Gln-Asp-Trp-Gly- Ala-His-Arg-Cys-Thr-NH2) (SEQ ID NO: 48)
[00260] [00260] Ac-Ile-Cys * -Val- (1Me) Trp-Gln-Asp-Trp-Gly-Ala-His-Arg- Cys * -Thr -NH-CH2CH2OCH2CH2OCH2-C (= O) -Lys-C ( = O) - (CH2CH2O) n-NH2 (SEQ ID NO: 49)
[00261] [00261] Ac-Ile-Cys * -Val- (1Me) Trp-Gln-Asp-Trp-Gly-Ala-His-Arg- Cys * -Thr-Lys-C (= O) - (CH2CH2O) n -NH2 (SEQ ID NO: 50).
[00262] [00262] Ac-Ile-Cys * -Val- (1Me) Trp-Gln-Asp-Trp-Gly-Ala-His-Arg-
[00263] [00263] Ac- (CH2CH2O) nC (= O) Lys- (Gly) 5-Ile-Cys * -Val- (1Me) Trp- Gln-Asp-Trp-Gly-Ala-His-Arg-Cys * -Thr - NH2) (SEQ ID NO: 52)
[00264] [00264] Ac- (CH2CH2O) nC (= O) Lys-Ile- Cys * -Val- (1Me) Trp-Gln-Asp- 5 Trp-Gly-Ala-His-Arg-Cys * -Thr - NH2) ( SEQ ID NO: 53)
[00265] [00265] In SEQ ID NO: 48, (CH2CH2O) n is coupled via an amide bond to the N-terminal amino acid. In SEQ ID NOs: 49-53, the (CH2CH2O) n portion is coupled via an amide bond to a Lys side chain; thus, it will be understood that the NH2 at the C-terminal in SEQ ID NOs: 49, 50, and 51, represents amidation of the C-terminal of the peptide, and it will be understood that in SEQ ID NOs: 52 and 53, the Ab in the N- terminal represents acetylation of the N-terminal of the peptide, as described above. It will be appreciated by those skilled in the art that a free end of a (CH2CH2O) n portion normally ends with an (OR) where the underlined O represents the O atom in the terminal group (CH2CH2O). (OR) is often a moiety such as a hydroxyl (OH) or methoxy (-OCH3) group, however, other groups (for example, other alkoxy groups) could be used. Thus, SEQ ID NO: 49, for example, can be represented as Ac-Ile-Cys * -Val- (1Me) Trp-Gln-Asp-Trp-Gly-Ala-His-Arg-Cys * -Thr- NH-CH2CH2OCH2CH2OCH2-C (= O) -Lys- (C (= O) - (CH2CH2O) n- R) -NH2 (SEQ ID NO: 54) where R is, for example, H or CH3 in the case of Linear PEG. In the case of a bifunctional, branched or star-shaped PEG, R represents the remainder of the molecule. In addition, it will be understood that the portion comprising the reactive functional group may vary, as described in this document (for example, according to any of the formulas described in this document). For example, long-acting compstatin analogs comprising the same peptide sequence as SEQ ID NO: 54, wherein the portion comprising the reactive functional group comprises an ester and / or alkyl chain can be represented as follows
[00266] [00266] Ac-Ile-Cys * -Val- (1Me) Trp-Gln-Asp-Trp-Gly-Ala-His-Arg- Cys * -Thr-NH-CH2CH2OCH2CH2OCH2-C (= O) -Lys- (C (= O) - (CH2) m- (CH2CH2O) nR) -NH2 (SEQ ID NO: 55); 5 [00267] Ac-Ile-Cys * -Val- (1Me) Trp-Gln-Asp-Trp-Gly-Ala-His-Arg- Cys * -Thr-NH-CH2CH2OCH2CH2OCH2-C (= O) -Lys- ( C (= O) - (CH2) m- C (= O) - (CH2CH2O) nR) -NH2 (SEQ ID NO: 56)
[00268] [00268] Ac-Ile-Cys * -Val- (1Me) Trp-Gln-Asp-Trp-Gly-Ala-His-Arg- Cys * -Thr-NH-CH2CH2OCH2CH2OCH2-C (= O) -Lys- (C (= O) - (CH2) m- C (= O) - (CH2) j (CH2CH2O) nR) -NH2 (SEQ ID NO: 57)
[00269] [00269] In EQ ID NOs: 55-57, m can vary from 1 to 2, 3, 4, 5, 6, 7, 8, 10, 15, 20 or 30 in various modalities. In SEQ ID NO: 57, j can range from 1 to about 2, 3, 4, 5, 6, 7, 8, 10, 15, 20 or 30 in various embodiments. In addition, it will be appreciated that, as described in this document, in various embodiments other portions can be incorporated between Lys- (C (= O) - and (CH2CH2O) nR, such as an amide, aromatic ring (for example, a substituted phenyl or unsubstituted), or a substituted or unsubstituted cycloalkyl structure.
[00270] [00270] In some embodiments, a long-acting compstatin analogue comprises a variant of SEQ ID NOs: 48-57 where -Ile-Cys * -Val- (1Me) Trp-Gln-Asp-Trp-Gly-Ala- His-Arg-Cys * -Thr- is replaced by an amino acid sequence comprising the amino acid sequence of any other compstatin analogue, for example, any of SEQ ID NOs 3-27 or 29-41, with the respects that the blocking portions present in the N- and / or C-terminal of a compstatin analogue may be absent, replaced by a linker (which may comprise a blocking portion), or linked to an amino acid of Different N- or C-terminal present in the corresponding variant (s).
[00271] [00271] Any compstatin analogue, for example, any compound that includes any of SEQ ID NOs: 3-41, can be linked through its N-terminal or C-terminal directly or indirectly to any portion comprising a group reactive functional, for example, any of Formulas I - XVI or Compound I-III, 5 in various modalities.
[00272] [00272] In some embodiments, a CRM comprises a polypeptide that occurs in human serum, or a fragment thereof, or a substantially similar variant of the polypeptide or fragment thereof. In some embodiments, the polypeptide, fragment, or variant has a molecular weight of between 5 kD and 150 kD, for example, at least 5, 10, 20, 30, 40, 50, 60, 70, 80, 90, 100 kD, or more, for example, between 100 and 120, or 120 and 150 kD. In some embodiments, producing a long-acting compstatin analog comprises reacting a compstatin analog comprising a reactive functional group with one or more amino acid side chains of the polypeptide, wherein the side chain comprises a compatible functional group. In some embodiments, producing a long-acting compstatin analog comprises reacting a compstatin analog comprising a functional group reactive with the N-terminal amine and / or the C-terminal carboxyl group of the polypeptide. In some embodiments, producing a long-acting compstatin analogue comprises reacting a compstatin analogue comprising an amine reactive functional group with amino acids having a side chain comprising a primary amine (e.g., lysine) and / or with the N- terminal of the polypeptide. In some embodiments, producing a long-acting compstatin analogue comprises reacting a compstatin analogue comprising a reactive carboxyl group with the C-terminal carboxyl group of the polypeptide. In some embodiments, a portion of compstatin analog is attached at each end of the polypeptide and, optionally, to the side chain of one or more internal amino acids.
[00274] [00274] In some embodiments, the polypeptide is produced recombinantly. In some embodiments, the polypeptide is at least partly produced recombinantly (for example, in 5 bacteria or in eukaryotic host cells such as fungi, insects, plants, or vertebrate animals) and / or at least in part, produced - made using chemical synthesis. In some embodiments, the polypeptide is purified. In some embodiments, the polypeptide is glycosylated. In some embodiments, the polypeptide is non-glycosylated. In some embodiments, the polypeptide is human serum albumin (HSA). In some embodiments, a substantially similar variant of the polypeptide is sufficiently similar to the polypeptide that it is a variant so as not to be recognized as foreign by a normal immune system of an individual, for example, a human individual. In some embodiments, changes in the sequence of the substantially similar variant when compared to the polypeptide of which it is a variant are selected in order to avoid MHC Class I epitopes. Several methods known in the art can be used to predict whether one sequence comprises an MHC Class I epitope.
[00275] [00275] The structure of compstatin is known in the art, and NMR structures for a number of compstatin analogs having higher activity than compstatin are also known (Malik, supra). Structural information can be used to design compstatin mimetics. In some embodiments, the compstatin mimetic is any compound that competes with compstatin or any compstatin analogue (for example, a compstatin analogue whose sequence is provided in Table 2) for binding to C3 or a fragment thereof ( for example, a 40 kD fragment of the b chain to which compstatin binds). In some embodiments, the compstatin mimetic has an activity equal to or greater than that of compstatin. In some embodiments, the compstatin mimetic is more stable, available orally, or has better bioavailability than compstatin. The compstatin mimetic can be a peptide, nucleic acid, or small molecule. In certain embodiments, the compstatin mimetic is a compound that binds to the compstatin binding site as determined in a compstatin-C3 structure, for example, a crystal structure or a 3-D structure derived from NMR experiments. In certain embodiments, the compstatin mimetic is a compound that could replace compostatin in a compstatin-C3 structure and would form substantially the same intermolecular contacts with C3 as compstatin. In certain embodiments, the compstatin mimetic is a compound that binds to a peptide binding site having a sequence set out in Table 1, for example, SEQ ID NO: 14, 21, 28, 29, 32, 33, 34, or 36 or other compstatin analogue sequence or in certain embodiments SEQ ID NO: 30 or 31, in a C3 peptide structure. In certain embodiments, the compstatin mimetic is a compound that could replace a peptide having a sequence set out in Table 1, for example, SEQ ID NO: 14, 21, 28, 29, 32, 33, 34, or 36 or another sequence of compstatin analog or in certain embodiments SEQ ID NO: 30 or 31, in a C3 peptide structure and would form substantially the same intermolecular contacts with C3 as the peptide. In certain modalities, the compstatin mimetic has a non-peptide main structure, but has side chains arranged in a sequence designed based on the compstatin sequence.
[00276] [00276] A person skilled in the art will appreciate that once a specific desired conformation of a short peptide has been verified, methods for designing a peptide or peptidomimetic to
[00277] [00277] It will be appreciated by those skilled in the art that a peptide mimic can serve as well as a peptide in order to provide the conformation of the specific main structure and side chain functionalities necessary to bind C3 and inhibit complement activation. In this sense, it is contemplated as being in the scope of the present invention to produce and use the C3 bond, complement inhibitory compounds through the use of naturally occurring amino acids, amino acid derivatives, analogs or non-amino acid molecules capable of be joined to form the conformation of the appropriate main structure. A non-peptide analogue, or an analogue comprising peptide and non-peptide components, is sometimes referred to in this document as "peptidomimetic" or "isosteric mimetic", to denote substitutions or derivations of a peptide that has many of the same conformational characteristics. main structure and / or other functionalities, in order to be sufficiently similar to the exemplified peptides to inhibit complement activation. More generally, a compstatin mimetic is any compound that would position pharmacophores similar to its placement in compstatin, even if the structure
[00278] [00278] The use of peptidomimetics for the development of high-affinity peptide is well known in the art. Assuming similar rotational restrictions of amino acid residues, within a peptide, analogues comprising portions of non-amino acids can be analyzed, and their conformational motifs verified, using the Ramachandran graph (Hruby & Nikiforovich 1991), among others known techniques.
[00279] [00279] A person skilled in the art will readily be able to establish adequate screening assays to identify additional compstatin mimetics and select those having desired inhibitory activities. For example, compstatin or an analogue of it could be labeled (for example, with a radioactive or fluorescent label) and placed in contact with C3 in the presence of different concentrations of a test compound. The ability of the test compound to decrease binding of the compstatin analogue to C3 is assessed. A test compound that significantly decreases the binding of the compstatin analogue to C3 is a candidate compstatin mimetic. For example, a test compound that decreases the steady-state concentration of a compstatin-C3 analog complex, or that decreases the rate of formation of a compstatin-C3 analog complex by at least 25%, or by at least 50%, it is a candidate compstatin mimetic. One skilled in the art will recognize that a number of variations of this screening test can be employed. The compounds to be screened include natural products, aptamer libraries, phage display libraries, compound libraries synthesized using combinatorial chemistry, etc. The invention encompasses synthesizing a combinatorial library of compounds based on the core sequence described above and screening the library to identify compstatin mimetics.
[00281] [00281] In some embodiments, a protease that degrades C3 can be used as a complement inhibitor. For example, Pat. US No. 6,676,943 discloses protein that degrades human complement C3 from Streptococcus pneumoniae. Such proteins, or variants thereof, can be used in certain modalities of the invention.
[00282] [00282] Pat. US No. 5,942,405, PCT / IB2006 / 002557 (WO / 2007/034277 -ARYL SUBSTITUTED IMIDAZO [4,5-C] PYRIDINE COMPOUNDS AS C3A RECEPTOR ANTAGONISTS); PCT / IB2006 / 002568 (WO / 2007/034282 -DIARYL-IMIDAZOLE COM- POUNDS CONDENSED WITH A HETEROCYCLE AS C3A RECEP-
[00283] [00283] In certain embodiments, a complement inhibitor inhibits factor B activation or activity. For example, the complement inhibitor can bind factor B and, for example, inhibit factor B activation. examples that inhibit factor B activation or activity include, for example, antibodies, antibody fragments, peptides, small molecules and aptamers. Exemplary antibodies that inhibit factor B are described in Pat. US Pub. No. 20050260198. In certain embodiments, an antibody or antigen-binding fragment selectively binds to factor B within the third domain of short consensus repeats (SCR). In certain embodiments, the antibody prevents the formation of a C3bBb complex. In certain embodiments, the antibody or antigen-binding fragment prevents or inhibits factor B cleavage by factor D. In some embodiments, an antibody binds to the Bb portion of factor B.PCT / US2008 / 074489 (WO / 2009/029669) discloses exemplary antibodies, p. , the antibody produced by the hybridoma clone deposited under ATCC Adhesion Number PTA-8543. In some embodiments, a humanized version of said antibody is used, which may be an antibody fragment. In certain embodiments, a complement inhibitor, for example, antibody, small molecule, aptamer, polypeptide or peptide, substantially binds to the same binding site on factor B as an antibody described in Pat. US Pub. No. 20050260198 or WO / 2009/029669. In some embodiments, the complement inhibitor comprises the fragment of monoclonal antibody, known as TA106 (previously under development by Taligen Therapeutics) or antibody, small molecule, aptamer, polypeptide or peptide, binds substantially to the same binding site on factor B that TA106 is used. In some embodiments, a peptide that binds to and inhibits factor B is identified using, for example, a method such as phage display. In some modalities, a complement inhibitor comprises an aptamer that binds to and inhibits factor B. In some modalities, an RNAi agent that inhibits the expression of factor B can be used. Compounds that inhibit Factor D Activation or Activity
[00284] [00284] In certain embodiments, the complement inhibitor inhibits factor D. For example, the complement inhibitor can bind to factor D. Exemplary agents include antibodies, antibody fragments, peptides, small molecules and aptamers. Axemplar antibodies that inhibit factor D are described in Pat. US No.
[00285] [00285] In some embodiments, the complement inhibitor comprises at least a portion of a mammal, for example, complement regulatory protein or human complement receptor. Examples of complement regulatory proteins, for example, CFH, CFH-related proteins (such as CFHR1), CFI, CR1, DAF, MCP, CD59, C4bp and complement 2 receptor trispanning inhibitor (CRIT; Inal, J., et al, J. Immunol., 174 (1): 356-66, 2005). In some embodiments, the complement regulatory polypeptide is one that is normally attached to the membrane in its natural state. In some embodiments of the invention, a fragment of such a polypeptide is used that lacks some or all of a transmembrane and / or intracellular domain. Soluble forms of complement 1 receptor (sCR1) or soluble portions of other complement receptors, for example, are of use in certain modalities. For example, compounds known as TP10 or TP20 (Avant Therapeutics) can be used. In some embodiments, a soluble complement control protein, for example, CFH or a CFH-related protein, is used. In some embodiments, the complement inhibitor is a Complement Receptor molecule C3b / C4b such as those described in Pat. US Pub. No. 20020192758. Variants and fragments of mammalian complement regulatory proteins or receptors that retain complement inhibitory activity can be used in certain embodiments.
[00286] [00286] In certain embodiments of the invention, the complement inhibitor comprises a chimeric polypeptide comprising a first polypeptide that inhibits complement activation, linked, for example, covalently linked, to a second polypeptide that inhibits complement activation and / or that attaches to an add-on component or add-on activation product. In some embodiments, at least one of the polypeptides comprises at least a portion of a mammalian complement regulatory protein. The chimeric polypeptide can contain one or more additional domains located, for example, between the first and the second polypeptide or at a terminal. For example, the first and second polypeptides can be separated by a spacer polypeptide.
[00287] [00287] In some embodiments, the first and second polypeptides each comprise at least a portion of a mammalian complement regulatory protein. In some embodiments, the complement inhibitor comprises at least a portion of DAF and at least a portion of MCP. Exemplary chimeric polypeptides are disclosed, for example, in Pat. US No. 5,679,546, for example, CAB-2 (also known as MLN-2222). In some embodiments, the polypeptide comprises at least 4 SCR domains of at least one mammalian complement regulatory protein or complement receptor. In some embodiments, the polypeptide comprises at least 4 SCR domains from each of the first and second complement regulatory proteins of different mammals.
[00288] [00288] In some embodiments, a chimeric polypeptide comprises at least a portion of complement 1 receptor (CR1), complement 2 receptor (CR2), complement 3 receptor (CR3), complement 4 receptor (CR4) or a variant or fragment
[00289] [00289] In some embodiments of the invention, other anti-protoperdine agents, antibody fragment or the other anti-protoperin agents are used. See, for example, Pat. US Pub. No. 20030198636 or PCT / US2008 / 068530 (WO / 2009/110918 -ANTI-PROPERDIN ANTI-BODIES) for examples. Compounds that Inhibit Lectin Pathway Components
[00290] [00290] In some embodiments, the compounds inhibit one or more components of the lectin pathway. See, for example, WO / 2007/117996) METHODS FOR TREATING CONDITIONS ASSO- CIATED WITH MASP-2 DEPENDENT COMPLEMENT ACTIVATION. Compounds that inhibit C5 Activation or Activity
[00291] [00291] In certain embodiments, the complement inhibitor inhibits C5 activation. For example, the complement inhibitor can bind to C5 and inhibit its cleavage. In some embodiments, the complement inhibitor inhibits the physical interaction of C5 with C5 convertase by, for example, binding to C5 or C5 convertase or C5 at a site that would normally participate in such a physical interaction. Exemplary agents that inhibit C5 activation include antibodies, antibody fragments, polypeptides, small molecules and aptamers. Exemplary compounds, for example, antibodies, which bind to C5 are described, for example, in Pat. US No. 6,534,058; PCT / US95 / 05688 (WO 1995/029697), PCT / EP2010 / 007197 (WO2011063980); Pat. U.S. Pub. No. 20050090448; and Pat. US Pub. No. 20060115476. Pat. US Pub. No. 5 20060105980 disclose aptamers that bind to and inhibit C5. In some embodiments, a humanized anti-C5 monoclonal antibody, for example, eculizumab (also known as h5G1.1-mAb; Solisris®) (Alexion), or a fragment or derivative thereof that binds to C5. In some embodiments, an antibody that includes at least some of the same complementarity determining regions (C-DR1, CDR2 and CDR3), for example, all of CDR1, CDR2 and CDR3, such as those of the heavy chain and / or light chain of eculizumab is used. In some embodiments, the antibody comprises at least some of the same framework regions as eculizumab. In some embodiments, an antibody that binds to substantially the same C5 binding site as eculizumab is used. In some embodiments, pexelizumab (also known as h5G1.1-scFv), a humanized, recombinant, single chain antibody derived from h5G1.1-mAb, is used. In certain embodiments, the complement inhibitor comprises a Staphylococcus SSL7 protein from Staphylococcus aureus or a variant or derivative or mimetic of such a protein that can bind to C5 convertase and inhibit its cleavage.
[00292] [00292] As indicated above, biospecific or multi-specific antibodies can be used. For example, PCT / US2010 / 039448 (WO / 2010/151526) discloses the biospecific antibodies described as binding to two or more different proteins, in which at least two of the proteins are selected from C5a, C5b, a receptor cell for C5a (for example, C5aR1 or C5L2), the C5b-9 complex and a terminal complement component or intermediate such as C5b-6, 7-C5b or C5b-8. In some embodiments, an RNAi agent that inhibits C5 or C5aR expression can be used.
[00293] [00293] In some modalities, a complement inhibitor known as OmCI, or a variant, derived, or mimetic, is used. OmCI binds to C5 and inhibits its activation, probably inhibiting the interaction with convertase. OmCI is produced naturally by the Ornithodoros moubata tick. See, for example, PCT / GB2004 / 002341 (WO / 2004/106369) and PCT / GB2010 / 000213 (WO / 2010/100396), for description of OmCI and certain respective variants. It has been shown that OmCI binds to eicosanoids, in particular leukotrienes (LKs), for example, LTB4. In some embodiments, an OmCI polypeptide (or a variant, derivative or fragment thereof) that retains the binding capacity for a LK, for example, LTB4, is used. In some embodiments, an OmCI polypeptide (or a variant, derivative or fragment thereof) that has reduced capacity or is substantially incapable of binding to LK, for example, LTB4, is used.
[00294] [00294] In some embodiments, the agent is an antagonist of the C5a (C5aR) receptor. In some embodiments, the C5aR antagonist comprises a peptide. Exemplary C5a receptor antagonists include a variety of small cyclic or acyclic peptides, such as those described in March, DR, et al., Mol. Pharmacol., 65 (4), 2004, and in Woodruff, TM, et al., J Pharmacol Exp Ther., 314 (2): 811-7, 2005, US Pat. No. 6,821,950; USSN 11 / 375,587; and / or PCT / US06 / 08960 (WO2006 / 099330), or a mimetic thereof. In certain embodiments, the complement inhibitor binds C5aR and inhibits the binding of C5a to them. In certain modalities, a cyclic peptide comprising the sequence [OPdCha-WR] (SEQ ID NO: 59) is used. In certain embodiments, a cyclic peptide comprising the sequence [KPdChaWR] (SEQ ID NO: 60) is used. In certain embodiments, a peptide comprising the sequence (Xaa) n [OPdChaWR] (SEQ ID NO: 61) is used, where Xaa is an amino acid residue and n is between 1 and 5. In certain embodiments, a peptide comprising the sequence (Xaa) n [KPdChaWR] (SEQ ID NO: 62) is used, where Xaa is an amino acid residue and n is between 1 and 5. In certain modalities, n is 1. In certain modalities, n is 1 and Xaa is a standard or non-standard aromatic amino acid. For example, the peptides F- [OPdChaWR] (SEQ ID NO: 63), F- [KPdChaWR] (SEQ ID NO: 64); CIN- [OPdChaWR] (SEQ ID NO: 65) and HCin- [OPdChaWR] (SEQ ID NO: 66) are useful in certain modalities. Optionally, the free terminal comprises a blocking portion, for example, the terminal amino acid is acetylated. For example, in some modalities, the C5aR antagonist is AcF- [OPdChaWR] (SEQ ID NO: 67) (also known as PMX-53). (Abbreviations: O: ornithine; Cha: cyclohexylalanine; CIN: cinnamoyl; Hcin: hydrokinamoyl; brackets denote internal peptide bond). In some embodiments, a C5aR antagonist comprises a compound, for example, a peptide, disclosed in Pat. US Pub. No. 20060183883 (USSN 10/564788), for example, a compound as represented by formula I, formula II, formula IV, formula V or formula VI. An exemplary C5aR antagonist is the peptide known as JPE-1375 (Jerini AG, Germany).
[00295] [00295] In some embodiments, a C5aR antagonist is a small molecule. Several small molecule C5aR antagonists are disclosed in the following references: PCT / US2005 / 015897 (WO / 2005/110416; 4,5-DISUBSTITUTED-2-ARYL PYRIMIDINES); PCT / EP2006 / 005141 (WO2006128670); PCT / US2008 / 072902 (WO / 2009/023669; SUBSTITUTED 5,6,7,8- TETRAHYDROQUINOLINE DERIVATIVES); PCT / US2009 / 068941 (WO / 2010/075257; C5AR ANTAGONISTS). An exemplary small molecule C5aR antagonist is CCX168 (ChemoCentryx, Mountain View, CA).
[00296] [00296] In certain embodiments, the complement inhibitor is an agent, for example, an antibody, small molecule, aptamer 5 or polypeptide, which binds substantially to the same binding site in C5 or C5aR as a compound described in any one of the aforementioned references disclosing agents that bind to C5 or C5aR. In some embodiments, the complement inhibitor is not a C5a receptor antagonist. Multimodal Complement Inhibitors
[00297] [00297] In certain embodiments of the invention, the complement inhibitor binds to more than one complement protein and / or inhibits more than one step in a complement activation pathway. Such complement inhibitors are referred to in this document as "multimodal". In certain embodiments of the invention, the complement inhibitor comprises a virus complement control protein (VCCP). The invention contemplates the use of any of the agents described in U.S.S.N. 11 / 247,886 and PCT / US2005 / 36547. Poxviruses and herpesviruses are large and complex virus families, with a linear double-stranded DNA genome. Some of these viruses encode immunomodulatory proteins that are believed to play a role in the pathogenesis by subverting one or more aspects of the normal immune response and / or promoting the development of a more favorable environment in the host organism (Kotwal, GJ, Immu - nology Today, 21 (5), 242-248, 2000). Among these are VCCPs. Poxvirus complement control proteins are members of the complement control protein (CCP) superfamily and typically contain 4 SCR modules. In certain modalities, VCCP is a poxvirus complement control protein (PVCCP). PVCCP may include a sequence encoded by, for example, vaccinia virus, major smallpox virus, minor smallpox virus, bovine smallpox virus, monkey pox virus, ectromelia virus, rabbit smallpox virus, myxoma virus , Yaba-like disorder virus or swinepox virus. In other modalities, VCCP is a herpesvirus complement control protein (HVCCP). HVCCP may include a sequence encoded by a Macaca fuscata rhadinovirus, cercopithecine herpesvirus 17, or human herpesvirus 8. In other embodiments, HVCCP is composed of a sequence encoded by herpes simplex saimiri virus ORF 4 or ORF 15 (Albrecht, JC . & Fleckenstein, B., J. Virol., 66, 3937-3940, 1992; Albrecht, J., et al., Virology, 190, 527-530, 1992).
[00298] [00298] VCCP can inhibit the classic complement pathway, the alternative complement pathway, the lectin pathway, or any two or more of these. In certain embodiments of the invention, VCCP, for example, a PVCCP, binds to C3b, C4b or both. In certain embodiments of the invention, PVCCP comprises one or more putative heparin binding sites (K / R-X-K / R) and / or has a positive overall load. In some embodiments, the PVCCP comprises at least 3 SCR modules (for example, modules 1-3), for example, 4 SCR modules. The PVCCP protein may be a precursor to a mature PVCCP (that is, they may include a signal sequence that is normally cleaved when the protein is expressed in virus-infected cells) or it may be a mature form (ie, lack the signal sequence).
[00299] [00299] Vaccinia complement control protein (VCP) is a virus-encoded protein secreted from vaccinia-infected cells. VCP is 244 amino acids long, contains 4 SCRs and is produced naturally by intracellular cleavage of a 263 amino acid pre-cursor. VCP is performed as a ~ 35 kD protein on a 12% SDS / polyacrylamide gel under reduced conditions
[00300] [00300] VCP also has the ability to bind strongly to heparin in addition to heparan sulfate proteoglycans. VCP contains two putative heparin binding sites located in modules 1 and 4 (Jha, P and Kotwal, GJ and references therein). VCP is able to bind to the surface of endothelial cells, possibly through interaction with heparin and / or heparan sulfate on the cell surface, resulting in decreased antibody binding (Smith, SA, et al., J.Viol. , 74 (12), 5659-5666, 2000). VCP can be taken by mast cells and possibly persist in the tissue for long periods of time, thus potentially prolonging its activity (Kotwal, GJ, et al., In GP. Talwat, et al. (Eds), 10th International Congress of Immunology., Monduzzi Editore, Bologna, Italy, 1998). In addition, VCP can reduce leukocyte chemotactic migration, blocking chemokine binding (Reynolds, D, et al., In S. Jameel and L. Villareal (ed., Ad-
[00302] [00302] Beef pox complement control proteins (referred to as inflammation-modulating protein, IMP) and monkey pox virus (referred to herein as monkey pox virus complement control protein, MCP) also They were also identified and sequenced (Miller, CG, et al., Virology, 229, 126-133, 1997 and Uvarova, EA and Shchelkunov, SN, Virus Res., 81 (1-2), 39-45, 2001). MCP differs from other PVCCPs described in this document, which contain a truncation of the C-terminal portion of the fourth SCR.
[00303] [00303] It will be appreciated that the exact sequence of complement control proteins identified in different isolated viruses may be slightly different. These proteins fall within the scope of the present invention. Complement control proteins from any such isolate may be used, provided the protein has not undergone a mutation that substantially abolishes its activity. Thus, the sequence of a VCCP such as SPICE or VCP may differ from the exact sequences presented in this document or under the membership numbers listed in Table 3. It will also be appreciated that a number of amino acid changes, for example, additions, exclusions or substitutions such as conservative amino acid substitutions, can be done on a typical polypeptide such as a VCCP without significantly affecting its activity, such that the resulting protein is considered equivalent to the original polypeptide. The viral polypeptides identified by the adhesion number in Table 3 below are in use in various embodiments of the invention. Table 3: Viral Complement Control Proteins Representatives Viruses Protein Adherence Virus Type Smallpox D12L NP_042056 Orthopoxvirus D15L (SPICE) AAA69423 Orthopoxvirus Vaccinia VCP AAO89304 Orthopoxvirus Bovine variola CPXV034 AAM13481 Orthopoxyvirus Antipoxy virus V4 control of the CAE00484 Orthopoxvirus complement Rabbit smallpox RPXV017 AAS49730 Orthopoxvirus Rhadinovirus Maca- JM4 AAS99981 Rhadinovirus ca fuscata (Herpesvirus) Cercopithecinae- Complement linkage NP_570746 Herpesvirus Herpesvirus Herpesvirus virusH2 virus (ORF4)
[00304] [00304] In addition to the VCCPs described above, there are several other viral proteins that interfere with one or more steps in a complement pathway. These proteins are also of use in certain embodiments of the present invention. Some of these proteins do not necessarily show clear homology to cell complement regulators known to date. For example, HSV-1, HSV-2, VZV, PRV, BHV-1, EHV-1, and EHV-4 encode versions of a conserved glycoprotein known as gC (Schreurs, et al., J Virol., 62, 2251 -2257, 1988; Mettenleiter, et al, J. Virol., 64, 278-286; 1990; Herold, et al., J Virol., 65, 1090-1098; 1991). With the exception of VZV, the gC protein encoded by these viruses binds to C3b (Friedman, et al., Nature, 309, 633-634,1984; Huemer, et al., Virus Res., 23, 271- 280, 1993) gC1 (from HSV-1) accelerates the decay of the classic C3 convertase pathway and inhibits the binding of properdin and C5 to C3. Purified EBV virions have an activity that accelerates the decomposition of the alternative C3 convertase pathway and serve as a cofactor for the complement 1 regulatory protein factor (Mold et al., J Exp Med, 168, 949-969, 1988). The proteins mentioned above are collectively referred to as virus complement interference proteins (VCIPs). By any of a variety of means, such as interference with one or more complement activation steps, accelerating the decay of a complement component, and / or enhancing the activity of a complement regulatory protein, these VCIPs are said complement inhibitors. Any of these proteins, or their derivatives, for example, fragments or variants thereof, can be used as a therapeutic agent in the invention. As is the case with VCCPs, it will be appreciated that the exact sequence of VCIPs identified in different isolated viruses may be slightly different. These proteins fall within the scope of the present invention.
[00305] [00305] In certain embodiments of the invention, a fragment or variant of a VCCP or VCIP is administered locally to a subject. Preferred fragments and variants of a PVCCP have at least one of the following activities: (i) ability to bind to C3, C3b or both; (ii) ability to act as a cofactor for C3 cleavage factor I; (iii) ability to bind to C4, C4b or both; (iv) ability to act as a cofactor for C4 cleavage factor I; (v) the ability to accelerate the decay of C3 convertase existing from the classical pathway, alternative pathway or both; (vi) ability to bind heparin; (vii) the ability to bind to heparan sulfate proteoglycans; (viii) ability to reduce leukocyte chemotactic migration; (ix) ability to block chemokine binding (for example, MIP-1), for example, with the surface of a cell (for example, a leukocyte or endothelial cell surface); (x) ability to inhibit binding of the antibody to MHC class I molecules; (xi) ability to inhibit the classical complement pathway; (xii) ability to inhibit the alternative complement pathway; and (xiii) ability to inhibit complement-mediated cell lysis. Preferred fragments and variants of PVCCP show complement binding activity
[00306] [00306] In certain embodiments of the invention, the PVCCP fragment or variant comprises at least 3 SCR modules (for example, modules 1-3), preferably 4 SCR modules. Preferably, each of the SCR modules exhibits a significant sequence identity for an SCR module, found in a natural PVCCP, for example, VCP or SPICE. Preferably, the various SCR modules are arranged in a N to C form in order to maximize the overall identity for a natural PVCCP. If the sequence of a PVCCP fragment or variant contains an SCR domain that differs from the SCR consensus sequence in one or more positions, in certain modalities of the invention, the amino acid (s) in one or more different positions ferentes is identical to that found in a corresponding position in more closely related SCRs found in a natural PVCCP. In certain embodiments, the PVCCP variant comprises at least one SCR module from a first PVCPP and at least one SCR module from a second PVCPP. In certain embodiments, the PVCCP variant comprises at least one SCR module from a PVCCP and at least one SCR from a mammalian complement control protein (RCA protein). Any number of SCR modules, for example, 1, 2, 3, 4 or more can come from any particular PVCCP or RCA protein in various embodiments of the invention. All of these combinations and permutations are contemplated, although not explicitly established.
[00307] [00307] Generally, a fragment or variant of VCCP or VCIP that occurs naturally has sufficient structural similarity to its natural counterpart, which is recognized by a polyclonal antibody that recognizes the natural counterpart. In certain embodiments of the invention, a fragment or variant of a VCCP has sufficient structural similarity with VCP or SPICE so that when its three-dimensional structure (structure is real or predicted) is superimposed on the structure of VCP or SPICE, the overlap volume is at least 70%, preferably at least 80%, more preferably at least 90% of the total volume of the VCP structure. A complete or partial three-dimensional structure of the fragment or variant can be determined by crystallizing the protein as described for VCP (Murthy, 2001). Alternatively, an NMR solution structure can be generated, performed by several fragments of VCP (Wiles, AP, et al., J. Mol. Biol. 272, 253-265, 1997). A modeling program like MODELER (Sali, A. and Blundell, TL, J. Mol. Biol, 234, 779- 815, 1993), or any other modeling program, can be used to generate a predicted structure . The model can be based on the VCP structure and / or any known SCR structure. The PROSPECT-PSPP suite of programs can be used (Guo, JT, et al., Nucleic Acids Res. 32 (Web Server Issue): W522-5, July 1, 2004). Similar methods can be used to generate a structure for SPICE.
[00308] [00308] Fragments or variants of VCCP or VCIP can be generated by all available means, a large number of which are known in the art. For example, VCCPs, VCIPs and fragments or their variants can be produced using DNA technology combined, as described below. A fragment of VCCP or
[00309] [00309] Even if you do not wish to be bound by any theory, it is likely that amino acid differences between natural PVCCPs will occur in positions that are relevant to checking for differences in specific properties, such as the ability to bind heparin, level activity, etc. For example, VCP and SPICE differ by only 11 amino acids, but SPICE has much greater activity as a cofactor for targeting C3b (for example, cleavage occurs at a much faster rate with SPICE than with VCP). The differences in amino acids are likely to be responsible for the differential activities of the two proteins. The amino acids in these positions are attractive candidates for alteration to identify variants that have even greater activity. Additional Complement Inhibitors
[00310] [00310] In some embodiments, a complement inhibitor is a naturally occurring complement regulatory protein of mammals or a fragment or derivatives thereof. For example, the complement regulatory protein can be CR1, DAF, MCP, CFH or CFI. In some embodiments of the invention, the complement regulatory polypeptide is one that is normally attached to the membrane in its natural state. In some embodiments of the invention, a fragment of such a polypeptide is used that lacks some or all of a transmembrane and / or intracellular domain. Soluble forms of the complement 1 receptor (sCR1), for example, are of use in the invention. For example, compounds known as TP10 or TP20 (Advantage Therapeutics) can be used. C1 inhibitor (C1-INH) is also used. In some embodiments, a soluble complement control protein, for example, CFH, is used. In some embodiments of the invention, the polypeptide is modified to increase its solubility.
[00311] [00311] In some embodiments, a complement inhibitor is a C1s inhibitor. For example, Pat. US No. 6,515,002 describes compounds (furanyl and thienyl amidines, heterocyclic amidines and guanidines) that inhibit C1s. Pat. US No. 6,515,002 and 7,138,530 describe heterocyclic amidines that inhibit C1s. Pat. US No. 7,049,282 describes peptides that inhibit activation of the classical pathway. Some of the peptides comprise or consist of WESNGQPENN (SEQ ID NO: 68) or KTISKAKGQPREPQVYT (SEQ ID NO: 69) or a peptide having significant sequence identity and / or three-dimensional structural similarity. In some embodiments, these peptides are identical or substantially identical to a part of an IgG or IgM molecule. Pat. US No. 7,041,796 discloses Complement C3b / C4b Receptor molecules and their uses to inhibit complement activation. Pat. US No. 6,998,468 discloses anti-C2 / C2a inhibitors of complement activation. Pat. US No. 6,676,943 discloses protein that degrades human complement C3 from Streptococcus pneumoniae. V. Anti-Th17 agents
[00312] [00312] An anti-Th17 agent is any agent that inhibits the formation, survival and / or activity of Th17 cells or that inhibits the production or biological activity of a Th17 effector cell molecule such as IL-17. In some embodiments, an anti-Th17 agent inhibits the development, proliferation, survival and / or maturation of Th17 cells. In some embodiments, an anti-Th17 agent inhibits the production and / or biological activity of IL-6, IL-21, IL-23, and / or IL-1β. In some embodiments, an anti-Th17 agent inhibits the production and / or activity of a Th17 effector cytokine, for example, IL-17A, 5 IL-17F, IL-21, and / or IL-22. Exemplary anti-Th17 agents include, for example, agents that bind to a Th17-associated cytokine or that bind to a receptor for a Th17-associated cytokine and, for example, block the receptor's interaction with the endogenous cytokine, but do not significantly activate the receiver. EXemplar anti-Th17 agents include, for example, antibodies, aptamers, soluble receptor fragments (e.g., soluble extracellular domain of the relevant cytokine receptor) or other polypeptides, peptides, small molecules, etc. In some embodiments, an anti-Th17 inhibitor comprises an antibody that lacks substantially the ability to activate complement. For example, the antibody may have less than 10%, less than 5%, or less than 1% complementary stimulating activity compared to full-length human IgG1. In some embodiments, the antibody comprises a CH2 domain that has reduced the ability to bind to C1q compared to the CH2 domain of human IgG1. In some embodiments, the antibody contains human IgG4 CH1, CH2 and / or CH3 domains and / or does not contain human IgG1 CH1, CH2 and / or CH3 domains.
[00313] [00313] In some embodiments, an anti-Th17 agent has a molecular weight of 1 kD or less. In some embodiments, an anti-Th17 person has a molecular weight between 1 kD and 2 kD, between 2 kD and 5 kD, between 5 kD and 10 kD, between 10 kD and 20 kD, between 20 kD and 30 kD, between 30 kD and 50 kD, between 50 kD and 100 kD, or between 100 kD and 200 kD.
[00314] [00314] In some embodiments, an anti-Th17 agent is composed of an adnectin, affibody, anticalin or other type of polypeptide sometimes used in the art instead of an antibody, in which the polypeptide binds to a cytokine associated with Th17 to cytokine receptor.
[00315] [00315] Several anti-Th17 agents, for example, agents that inhibit one or more cytokines associated with Th17, are known in the art and can be used in several modalities.
[00316] [00316] Polypeptide sequences of interest in this document, for example, cytokines associated with Th17 and its receptors, are known in the art and available in public databases such as those available through Entrez at the National Center for Biotechnology Information (www. ncbi.nih.gov) or Universal Protein Resource (www.uniprot.org). Exemplary databases include, for example, GenBank, RefSeq, Gene, Protein, Nucleotide, Uni- ProtKB / SwissProt, UniProtKB / Trembl and the like. In general, sequences, for example, mRNA and polypeptide sequences, in the NCBI Reference Sequence database can be used as gene product sequences for a gene of interest. Such sequences can be used, for example, to produce a polypeptide useful as an antigen or reagent for the production, isolation or characterization of an agent that binds to the gene product. It will be appreciated that multiple alleles of a gene can exist between individuals of the same species. For example, differences in one or more nucleotides (for example, up to approximately 1%, 2%, 3-5% of nucleotides) in nucleic acids encoding a particular protein may exist between individuals in a particular species. Due to the degeneracy of the genetic code, such variations often do not alter the encoded amino acid sequence, although DNA polymorphisms that lead to changes in the sequence of the encoded proteins may exist. Examples of polymorphic variants can be found, for example, in the Single Nucleotide Polymorphism Data-base (dbSNP), available on the NCBI website at www.ncbi.nlm.nih.gov/
[00317] [00317] Table 4 lists Gene Identification and NCBI RefSeq action numbers for certain cytokines associated with human Th17 and their receptors. It will be appreciated that some of the protein sequences are precursor sequences. The mature form of the protein would lack a secretion signal sequence present in the precursor. It will be appreciated that the sequences described under the respective adhesion numbers for the cytokine and cytokine receptors listed in Table 4 are exemplary and that naturally occurring variants, for example, allelic variants, are encompassed in several modalities. In addition, it will be appreciated that for the purpose of generating a useful binding agent (for example, an antibody) for use as a detection reagent or therapeutic agent, sequences of variants, short peptide segments, etc., can be used in certain modalities. Table 4: Gene Identification and NCBI RefSeq Adhesion Numbers for Certain Th17-Associated Cytokines and Their Proteins
[00318] [00318] In some embodiments, an anti-Th17 agent is an anti-IL-23 agent. An IL-23 agent is an agent (for example, a molecule or complex) that partially or totally blocks, inhibits, neutralizes, prevents or interferes with a biological activity of IL-23. In some modalities, a biological activity sw IL-23 is the ability to induce the production of IL-17 by activated T cells. IL-23 is a heterodimeric cytokine composed of two subunits. The beta subunit of IL-23, also called p40, is shared with another cytokine, interleukin-12 (IL-12). The alpha subunit of IL-23 is also called p19. IL-23 subunits are joined by a disulfide bond. IL-23 signals through binding to a hetero-dimeric receptor, composed of IL-12Rbetal (IL12RB1), which is shared by the IL-12 and IL-23R receptor (Parham C, et al. (2002) J. Immunol 168 (11): 5699-708). IL-23R is constitutively associated with Janus kinase 2 (JAK2) and also binds to the STAT3 transcription activator in a ligand-dependent form. The signal transduction cascade of
[00319] [00319] Certain anti-IL-23 agents and methods of identifying and / or producing such agents are disclosed in USSN 10 / 697,599. For example, screening methods and assays that can be easily employed by the person skilled in the art to identify and / or produce a variety of anti-IL-23 agents (sometimes referred to as "IL-23 antagonists" in USSN 10 / 697,599) are disclosed.
[00320] [00320] In certain embodiments, an antibody an anti-IL-23 that binds to the p40 subunit of IL-23 is ustekinumab or a fragment thereof. Ustekinumab (experimental name CNTO 1275, trade name Stelara®, Centocor; CAS number: 815610-63-0) is a human monoclonal antibody of the IgG1 subclass. Exemplary anti-IL-23 antibodies that bind to the human IL-23 p19 subunit and isolated nucleic acids encoding at least one anti-IL-23p19 antibody, vectors, host cells and production methods are described in USSN 11 / 617,503. Additional anti-IL-23 antibodies that bind with the p19 subunit are described in USSN11 / 762,738.
[00321] [00321] In some embodiments, an anti-IL-23 agent comprises a protein derived from IL-23p40 specific immunoglobulin (see, for example, USSN 11 / 768,582).
[00322] [00322] In some embodiments, an IL-23 inhibitor comprising
[00323] [00323] In some embodiments, IL-23 activity is inhibited by interfering with IL-23 signal transduction, for example, by inhibiting one or more processes or proteins involved in the IL-23 signal transduction pathway. For example, in some embodiments, IL-23 signaling is inhibited using either a JAK inhibitor or a STAT inhibitor. In some embodiments, a JAK inhibitor inhibits the expression of JAK. Methods of use to inhibit JAK expression in some embodiments include the use of RNAi agents (eg, siRNA) or antisense oligonucleotides. In some embodiments, a JAK inhibitor inhibits the binding of JAK to the IL-23 receptor. In some embodiments, a JAK inhibitor inhibits the dimerization of JAK. In some embodiments, a JAK inhibitor inhibits the activity of the JAK kinase. For example, in some embodiments, a JAK inhibitor binds to the JAK kinase domain, for example, to the ATP binding site. Numerous JAK inhibitors are known in the art. For example, INCB028050 is an orally available JAK1 / JAK2 inhibitor with reported nanomolar potency against JAK1 (5.9 nM) and JAK2 (5.7 nM) (Fridman, JS, et al, J Immunol. 2010; 184 ( 9): 5298-307). INCB028050 is reported to inhibit intracellular signaling of multiple pro-inflammatory cytokines, including IL-6 and IL-23 at concentrations <50 nM. Small molecule inhibitors of JAK2 include, for example, AZD1480 and AZ960.
[00324] [00324] In some modalities, a STAT inhibitor inhibits the ex-
[00325] [00325] In some embodiments, an anti-Th17 agent is an anti-IL-17 agent. An agent IL-17 is an agent (for example, a molecule or complex) that partially or totally blocks, inhibits, neutralizes, prevents or interferes with a biological activity of IL-17. Exemplary anti-IL-17 polypeptides, for example, anti-IL-17 antibodies, are described in, for example, USSN 11 / 658,344. Additional anti-IL-17 antibodies are described in USSN 11 / 762,738. In some embodiments, an anti-IL-17 agent comprises at least a portion of an IL-17 receptor, the portion of which binds to IL-17. Exemplary IL-17 receptor polypeptides are disclosed in, for example, USSN 09 / 022.260.
[00326] [00326] It will be understood that a polypeptide comprising a binding domain of any of the various anti-Th17 antibodies or other polypeptides described herein can be transferred to other main polypeptide structures or used as isolated agents in certain embodiments. It will also be understood that the variants can be used. For example, a variant can be at least 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or more identical to a receptor binding domain . In some embodiments, an antibody that competes with a specific antibody known in the art for binding to a cytokine of interest can be used. In some modalities, an IgG class antibody is modified so that it does not lack an Fc domain that can activate the complement. For example, an IgG1 antibody variable domain can be transplanted into a constant region of an IgG4 antibody. SAW. Pharmaceutical Compositions and Administration Approaches
[00327] [00327] Appropriate preparations, for example, substantially pure preparations of a complement inhibitor, can be combined with pharmaceutically acceptable carriers or vehicles, etc., to produce a suitable pharmaceutical composition. The term "pharmaceutically acceptable carrier or vehicle" refers to a non-toxic carrier or vehicle that does not destroy the pharmacological activity of the compound with which it is formulated. One skilled in the art will understand that a carrier or vehicle is "non-toxic" if it is compatible with administration to a subject in an adequate amount to deliver the compound without causing undue toxicity. Pharmaceutically acceptable carriers or vehicles that can be used include, but are not limited to, water, saline, Ringer's solution, sodium acetate or potassium acetate solution, 5% dextrose and the like. The composition may include other components
[00328] [00328] In some embodiments, the invention provides a suitable complement inhibitor or a pharmaceutically acceptable composition comprising a complement inhibitor, packaged together with a package insert (label) approved by a government agency responsible for regulating pharmacists, for example. example, the US Food & Drug Administration. In some embodiments, the invention provides a pharmaceutical package comprising: (a) a pharmaceutically acceptable complement inhibitor in concentrated or solid form (for example, as a lyophilized powder); (b) a pharmaceutically acceptable carrier, diluent or vehicle. In some embodiments, a carrier, thinner or vehicle is suitable for delivering the composition using a nebulizer. In some instances, a suitable carrier, thinner, or vehicle may be provided separately or purchased by a healthcare provider from an appropriate source. Optionally, a package contains instructions for dissolving or diluting the complement inhibitor in the carrier, diluent or vehicle to produce a composition for administration. In some modalities, a package insert states one or more indications that include one or more chronic disorders measured by complement, for example, one or more chronic respiratory disorders, for example, asthma or COPD. In some modalities, the package insert informs specific characteristics of the patient and / or the disorder or criteria that define a patient population or category of disorder for the treatment for which the composition has been approved for use. In some embodiments, the package insert specifies that the composition can be or should be administered according to a method of the present invention, for example, according to a dosage plan and / or using a dosage range described herein.
[00329] [00329] In general, a pharmaceutical composition can be administered to a subject by any appropriate route of administration, including, but not limited to, intravenous, intramuscular, subcutaneous, respiratory, etc. In some modalities, local administration for a tissue or organ affected by a complement-mediated disorder is used. It will be understood that "administration" encompasses directly administering a compound or composition to a subject, instructing a third party to administer a compound or composition to a subject, prescription or suggesting a compound or composition to a subject (for example, for self-administration), self-administration and, if necessary, other means of making a compound or making a compound available to a subject. If administration is performed through an implanted reservoir, administration can be referred to as causing the release of a composition or compound from the reservoir.
[00330] [00330] Pharmaceutical compositions suitable for injectable use (for example, intravenous, subcutaneous or intramuscular administration) usually include sterile aqueous solution (where water is soluble) or sterile dispersions and powders for the extemporaneous preparation of sterile and injectable solutions or dispersion. Sterile solutions can be prepared by incorporating the compound in the required amount in an appropriate solvent, optionally with one or a combination of ingredients such as buffers such as acetates, citrates, lactates or phosphates; agents for the regulation of tonicity, such as sodium chloride or dextrose; antibacterial agents, such as benzyl alcohol or methyl parabens; antioxidants such as ascorbic acid, glutathione or sodium bisulfite; chelating agents such as ethylene diaminetetraacetic acid; and other suitable ingredients, etc., as desired, followed by filter-based sterilization. Those 5 versed in the technique will be aware of the numerous physiologically acceptable compounds that can be included in a pharmaceutical composition. Other useful compounds include, for example, carbohydrates, such as glucose, sucrose, lactose; dextrans; amino acids, such as glycine; polyols, such as mannitol. These compounds can, for example, serve as bulking agents and / or stabilizers, for example, in a powder and / or when part of the manufacturing or storage process involves lyophilization. Surfactant (s) such as Tween-80, Pluronic-F108 / F68, deoxycholic acid, phosphatidylcholine, etc., can be included in a composition, for example, to increase solubility or to provide microemulsion to deliver hydrophobic drugs. The pH can be adjusted with acids or bases, such as hydrochloric acid or sodium hydroxide, if desired. The parenteral preparation can be placed in ampoules, disposable syringes or infusion bags or multi-dose vials made of glass or plastic. Preferably, solutions for injection are sterile and acceptably free of endotoxins.
[00331] [00331] Generally, dispersions are prepared by incorporating the active compound in a sterile vehicle that contains a basic dispersion medium and other appropriate ingredients from those listed above. In the case of sterile powders for the preparation of sterile injectable solutions, preparation methods may include vacuum drying and freeze drying which produces a powder of the active ingredient plus any additional desired ingredient, for example, from a previously filtered sterile solution thereof.
[00332] [00332] For administration by respiratory route (inhalation), a complement inhibitor can be delivered in the form of an aerosol spray from a pressed container or dispenser that contains an appropriate propellant. A nebulizer, dry powder inhaler or metered dose inhaler (MDI) can be used. The aerosol may comprise liquid particles and / or dry particles (for example, 5 dry powders, large porous particles, etc.). Suitable aqueous vehicles useful in various modalities include water or saline, optionally including an alcohol. In some embodiments, the composition comprises a surfactant suitable for introduction into the lung. Other excipients suitable for pulmonary administration can be used.
[00333] [00333] A variety of different devices are available for respiratory administration. Nebulizers are devices that transform drug solutions or suspensions into aerosols that are suitable for deposition in the lower airway. Nebulizer types include jet nebulizers, ultrasonic wave nebulizers, and vibrating mesh nebulizers. A partial list of available vibrating mesh nebulizers includes eFlow (Pari), i-Neb (Respironics), MicroAir (Omron), IH50 Nebulizer (Beurer), and Aerosp® (Aerogen). A Respimat® Soft Mist ™ Inhaler (Boeringer Engage) can be used. A metered dose inhaler (MDI) is a portable aerosol device that uses a propellant to deliver the therapeutic agent. MDIs include a pressurized metal cylinder containing a pharmacological agent in suspension or solution, propellant, surfactant (usually) and a metering valve. Chlorofluorocarbons (CFCs) were widely used as propellants, but have been largely replaced by hydrofluorocarbons (HFCs, also known as hydrofluoroalkanes (HFA)) such as HFC-134a and HFC-227ea. Carbon dioxide and nitrogen are other alternatives. A dry powder inhaler (DPI) is a breathing activated device that delivers the drug in the form of particles contained in a bottle.
[00334] [00334] Inhalation accessory devices (IADs) generally fall into two categories: spacers and holding chambers. Spacers and holding chambers extend the mouthpiece of the inhaler and direct the medication mist towards the mouth, reducing the amount of medication lost in the air. Using a spacer with an MDI can help reduce the amount of drug that sticks to the back of the throat, improve the direction and deposition of the medication delivered by MDI. Valve retention chambers (HCVs) allow a thin cloud of medication to remain in the spacer until the patient breathes through a unidirectional valve, pulling the dose of the medicine into the lungs. Examples include Aerochamber and Optichamber.
[00335] [00335] Compositions of particulate material can be characterized based on several parameters such as the fraction of fine particles (FPF), the emitted dose, the average particle density and the average aerodynamic diameter of the mass (MMAD). Suitable methods are known in the art, some of which are described in Pat. US No. 6,942,868 and 7,048,908 and US Publication No. 20020146373, 20030012742 and 20040092470. In certain embodiments, aerosol particles are between approximately 0.5 m -10 m (MMAD), for example, about 5 m for respiratory delivery , although less
[00336] [00336] A dry particle composition containing particles smaller than about 1 mm in diameter is also referred to in this document as a dry powder. A "dry" composition has a relatively low liquid content, so that the particles are readily dispersible, for example, in a dry powder inhalation device to form an aerosol or spray. A "powder" is composed, in large part or essentially, entirely of finely dispersed solid particles that are relatively fluid and capable of being easily dispersed in an inhalation device and subsequently inhaled by a subject, preferably so that a significant fraction of the particles - cells can reach a desired part of the respiratory tract. In certain modalities, large porous particles having average geometric diameters ranging between 3 and 15 m and apparent density between 0.04 and 0.6 g / cm3 are used. See, for example, Pat. US No. 7,048,908; Edwards, D. et al, Science 276: 1868-1871, 1997; and Vanbever, R., et al., Pharmaceutical Res. 16: 1735-1742, 1999).
[00337] [00337] Various considerations for respiratory delivery that may be useful in embodiments of the present invention are discussed in Bisgaard, H., et al., (Eds.), Drug Delivery to the Lung, Vol. 26 in "Lung Biology in Health and Disease ", Marcel Dekker, New York, 2002. Aerosol devices are discussed, for example, in Dolovich MB, Dhand R. Lancet. (2011) 377 (9770): 1032-45.
[00338] [00338] Oral administration can be used in certain modalities. Oral compositions generally include an inert diluent or an edible carrier. For the purpose of administering oral therapy, the active compound can be incorporated with excipients and used in the form of tablets, troches or capsules, for example,
[00339] [00339] For topical application, a complement inhibitor can be formulated in an appropriate ointment that contains the active component suspended or dissolved in one or more carriers. Carriers for topical administration include, but are not limited to, mineral oil, liquid petrolatum, white petrolatum, propylene glycol, polyoxyethylene, compound polyoxypropylene, wax emulsion and water. Alternatively, pharmaceutically acceptable compositions can be formulated as an appropriate lotion or cream that contains a compstatin analogue suspended or dissolved in one or more pharmaceutically acceptable carriers. Suitable carriers include, but are not limited to, mineral oil, sorbitan monostearate, polysorbate 60, cetyl ester wax, cetearyl alcohol, 2-octyldodecanol, benzyl alcohol and water.
[00340] [00340] Systemic administration can also be through trans-mucous or transdermal means. For transmucosal or transdermal administration, penetrants suitable for the barrier to be permeated by
[00341] [00341] The compounds can also be prepared in the form of suppositories (for example, with conventional suppository bases such as cocoa butter and other glycerins) or retention enemas for rectal delivery.
[00342] [00342] Methods of local administration to the eye include, for example, intraocular administration, for example, intraocular injection, for example, intravitreal injection. In some modalities, administration is by choroidal injection, transscleral injection, eye drops or ointment for the eye, transretinal, subconjunctival bulbar, intravitreal injection, supracoroidal injection, subtenonian injection, scleral bag or scleral incision injection.
[00343] [00343] In certain embodiments of the invention, a complement inhibitor is prepared with carrier (s) that will protect the compound against rapid elimination from the body, such as a controlled release formulation, including implants and microencapsulated delivery systems. . For example, a compound can be incorporated or encapsulated in a microparticle or nanoparticle formulation. Biodegradable, biocompatible polymers can be used, such as vinyl acetate, polyanhydrides, polyglycolic acid, collagen, polyiortoesters, polyethers, polylactic acid, PLGA etc. Liposomes or other
[00344] [00344] In some embodiments, a complement inhibitor is used in combination with one or more additional active agents useful to treat a disorder of interest in this document (see, for example, Brunton, LL, et al. (Eds.) , Goodman and Gilman's The Pharmaco-logical Basis of Therapeutics, (for example, 11th or 12th edition), Mc- Graw-Hill, for examples of such agents.) In some modalities, one or more additional active agents are administered in the same composition as a complement inhibitor. In some embodiments, one or more additional active agents are administered in a separate composition, which separate composition can be administered before, at approximately the same time as, or after administration of a complement inhibitor. In some modalities, the use of a complement inhibitor allows a reduction in the dose and / or frequency of administration of an additional active agent, maintaining at least equivalent control of the disorder and / or benefit for the subject. It will be understood that pharmaceutical compositions comprising an additional active agent can be prepared using pharmaceutically acceptable carriers and / or preparation methods described herein or known in the art and administered using administration routes described herein or known in the art.
[00345] [00345] In some modalities, a second active agent is an agent that interferes with the cycle of DC-Th17-B-Ab-C-DC by a mechanism distinct from direct inhibition of complement component or activation of complement. In some embodiments, a second active agent can be an anti-IL-23 agent or an anti-IL-17 agent. In some embodiments, a pharmaceutical composition or pharmaceutical package comprises a second active agent that interferes with the cycle of DC-Th17-B-Ab-C-DC. In some embodiments, a package insert specifies that two agents should be administered in combination. In some embodiments, a complement inhibitor, for example, a compstatin analog, can be added to any treatment regimen that includes an anti-Th17 agent. In some embodiments, such an addition allows for a lower dose or a larger dosage range of the anti-Th17 agent to be used, without reducing effectiveness. In some embodiments, such an addition results in greater effectiveness.
[00346] [00346] When two or more therapies (for example, compounds or compositions) are used or administered "in combination" with each other, they can be given at the same time, within overlapping periods of time or in sequence (for example , separated by up to 2-4 weeks in time), in various modalities of the invention. They can be managed through the same route or different routes in several modalities. They can be administered in any order in various ways. In some embodiments, the compounds or compositions are administered within 4, 8, 12, 24, 48, 72, or 96 hours each. In some embodiments, a first agent is administered before or after the administration of the second person, for example, sufficiently close to the time when the two agents are present at useful levels within the body at least once. In some embodiments, the agents are administered sufficiently close to each other in time so that no more than 90% of the composition previously administered has been metabolized to inactive metabolites or eliminated, for example, excreted from the body, in the body. time when the second compound or composition is administered. In some embodiments, agents administered sufficiently close to each other in time so that no more than 2 weeks have passed since the previously administered agent has been metabolized to inactive metabolites or eliminated, for example, excreted from the body, at the time the second agent is administered. In some embodiments, the administration of two agents (for example, a complement inhibitor and a second agent that interferes with the DC-Th17-B-Ab-C-DC cycle act additively, resulting in an effect that cannot be achieved by any agent alone In some embodiments, the administration of two agents (for example, a complement inhibitor and a second agent that interferes with the DC-Th17-B-Ab-C-DC cycle act in synergy, resulting in in an effect that is greater than an additive effect and / or qualitatively different from an additive effect in a clinically and / or statistically significant way.
[00347] [00347] It will be noted that a complement inhibitor and / or additional active person (s) can be provided as a pharmaceutically acceptable salt. Pharmaceutically acceptable salts including those derived from pharmaceutically acceptable organic and inorganic acids and bases. Examples of suitable acid salts include acetate, adipate, alginate, aspartate, benzoate, benzenesulfonate, bisulfate, butyrate, citrate, camphorate, camphorsulfonate, cyclopentanepro-
[00348] [00348] It will be understood that the pharmaceutically acceptable carriers, compounds and methods of preparation mentioned in this document are exemplary and not restrictive. See, for example, Richmond: The Science and Practice of Pharmacy. 21st Edition. Philadelphia, PA. Lippincott Williams & Wilkins, 2005, for further discussion of pharmaceutically acceptable compounds and methods of preparing pharmaceutical compositions of various types.
[00349] [00349] A compound or composition, for example, a pharmaceutical composition, can be used or administered to a subject in an effective amount. In some embodiments, an "effective" amount of an active agent, for example, a complement inhibitor, (or composition containing an active agent) refers to an amount of the active agent (or composition) sufficient to cause one or more biological responses of interest to, for example, a subject to whom the active agent (or composition) is administered. As will be appreciated by those skilled in the art, the absolute value of a particular agent that is effective can vary depending on factors such as the biological end, the specific active agent, the target tissue, etc. Those skilled in the art will also understand that an "effective amount
[00350] [00350] Indicators of airway inflammation include, for example, the presence of a greater number of cells associated with inflammation, such as white blood cells (for example, eosinophils, lymphocytes, macrophages and / or neutrophils) and / or inflammatory mediators (for example, chemokines (for example, flunisolide, thymus and pro-activation regulated chemokines (TARC), macrophage-derived chemokines (MDC)), cytokines (for example, TNF, IL-1beta, IL-4, IL-5, IL-13, IL-25), histamine, cysteinyl leukotrienes, nitric oxide) in the airways, compared to an appropriate reference level, for example, a normal level. For example, the number and / or concentration of cells and / or mediators may be above the upper limit of the normal range in subjects who do not suffer from a disorder (where "normal range" usually refers to an interval within ± 2 standard deviations from an average value of a population of subjects) or can be greater than a value (or average value) measured in that subject, when the subject's disorder is well controlled. A reduction in the severity and / or frequency of symptoms can be statistically significant and / or clinically significant within the judgment of a physician or other medical professional. Determining whether a disorder is well controlled is up to the judgment of a physician or other medical professional. Guidelines accepted in the technique can be used. 5 [00351] In some modalities, an effective amount results in the reduction of at least one parameter associated with Th17 cells and / or Th17 activity. In some embodiments, an effective amount reduces the level of at least one cytokine associated with Th17 cells and / or Th17 activity, for example, a cytokine that promotes Th17 cell formation and / or activity or a cytokine produced by cells Th17. In some embodiments, a cytokine is IL-17, IL21, IL-22, or IL-23. In some modalities, an effective amount results in a change from Th17 to Treg cells. In some modalities, the exchange of Th17 cells for Treg cells is reflected in an immune microenvironment that is relatively rich in IL-10 and relatively poor in IL-17 and IL-23.
[00352] [00352] For the treatment of AMD, an effective amount may be sufficient to achieve one or more of the following: (i) inhibit or prevent the formation of druses; (ii) cause a reduction in the number and / or size of druses (dredge regression); (iii) causing a decrease or avoiding lipofuscin deposits; (iv) inhibit or prevent visual loss or decrease the rate of visual loss; (v) inhibit choroidal neovascularization or decrease the rate of choroidal neovascularization; (vi) cause a reduction in the size and / or number of lesions characterized by choroidal neovascularization; (vii) inhibit choroidal neovascularization or decrease the retinal neovascularization rate; (viii) cause a reduction in the size and / or number of lesions characterized by neovascularization of the retina; (ix) improving visual acuity and / or contrast sensitivity; (x) inhibit or prevent photoreceptor or atrophy of RPE cells or apoptosis or reduce the rate of photoreceptor or atrophy of RPE cells or apoptosis; (xi) inhibit or prevent the progression of non-exudative macular degeneration to exudative macular degeneration; (xii) reduce one or more signs of inflammation, for example, the presence of cells associated with inflammation, such as white blood cells (for example, neutrophils, macrophages) in the eye, the presence of inflammatory mediators endogenous, one or more symptoms such as eye pain, redness, sensitivity to light, blurred vision, and floaters, etc.
[00353] [00353] An individual skilled in the art will be aware of the appropriate methods to assess the biological effects mentioned above and other biological effects of interest. Symptoms can be assessed using standardized instruments (for example, questionnaires), known in the art. Any of a variety of different health-related quality of life (HRQoL) instruments can be used, which can be generic or specifically associated with the respiratory system (for example, asthma and / or COPD specifics). Pulmonary function tests, spirometry, in particular, can be used to measure pulmonary function parameters that are frequently changed in individuals with chronic respiratory diseases, such as FEV1, FVC, FEV1 / FVC, PEF, etc. Allergen challenge can be performed, for example, as described in Kelly MM. J Allergy Clin Immunol. 125 (2): 349-356, 2010 or studies described in Cockcroft, DW, et al. Can Respir J. 14 (7): 414–418, 2007. Myofibroblasts synthesize collagen and are believed to play an important role in airway remodeling in diseases characterized by chronic inflammation of the airways such as asthma and COPD. These cells increase in the airways of asthmatic individuals 24 h after the allergen challenge. Inhibition of the increase in airway wall myofibroblasts that would otherwise occur after the allergen challenge may indicate decreased airway remodeling potential. As an alternative or in addition, resources associated with airway remodeling such as smooth muscle hyperplasia, goblet cell hyperplasia, and / or subepithelial collagen deposition can be assessed. 5 [00354] Bronchial hyperreactivity can be assessed using, for example, "direct" and "indirect" challenge tests, which refer to the agents' mode of action in relation to smooth muscle contraction. Histamine diphosphate and methacholine chloride and are most commonly used as direct smooth muscle stimuli. The most frequently used indirect stimuli are hypertonic saline, adenosine monophosphates (AMP) and mannitol. Challenge tests can be performed, for example, according to guidelines published by the ERS (Sterk PJ, et al. Airway responsiveness. Standardized challenge testing with pharmacological, physical and sensitizing stimuli in adults. Report Working Party Standardization of Lung Function Tests , European Community for Steel and Coal. Official Statement of the European Respiratory Society. Eur Respir J Suppl 1993; 16: 53–83) and ATS (Crapo, RO, et al., Guidelines for methacholine and exercise challenge testing-
[00355] [00355] Cells associated with inflammation and / or mediators may be evaluated, for example, in a suitable sample such as induced sputum, BAL fluid, and / or airway tissue samples (for example, obtained from a biopsy , such as an endobronchial biopsy). Cells, for example, cells associated with inflammation can optionally be detected and quantified, using, for example, scanning electron microscopy, optical microscopy (optionally using suitable chemical stains or antibodies for specific markers (immunohistochemistry) ), flow cytometry or other appropriate methods Mediator levels (eg cytokine) can be measured using, for example, antibody-based assays, such as ELISA assays, flow matrix assays (eg, Luminex technology xMAP or flow cytometry (CBA - cytometric beads array) BD Biosciences system), antibody matrix assays or appropriate bioassays Expression of mediators can be evaluated alternatively or additionally by measuring the level of mRNA encoding such mediators , using any suitable method to measure the level of RNA such as PCR reverse transcription, hybridization to oligonucleotide or cDNA, RNA arrays -Seq (for example, methods using high-throughput sequencing technologies to sequence cDNA to obtain information about RNA in a sample), etc.).
[00356] [00356] Exercise tolerance can be assessed, for example, through the performance test in a 6-minute walk test (for example, in which improved exercise tolerance is evidenced by an increase in distance that a subject is capable of walking in 6 minutes), a controlled walk test (shuttle walk test), and / or cardiopulmonary exercise test. See, for example, ATS Statement: Guidelines for the Six-Minute Walk Test (2002) for a 6-minute walk test discussion.
[00357] [00357] In general, a control subject can be, for example, an untreated subject or a subject treated with a placebo. An "untreated subject" can be a subject who has not received treatment with a complement inhibitor in the previous 6 months. In some modalities, an untreated subject has not received treatment with an ICS, OCS, LTRA, or LABA for at least the previous 4 weeks. In some embodiments, an untreated subject has not received treatment with an anti-IgE agent for at least the previous 12 weeks. Historical control information can be used. In some modes, a subject can serve as his own control. For example, one or more parameters can be measured one or more times before treatment and again during and / or after treatment. In some modalities, an "active control" (or "active comparator") is used, in which a biological effect of the complement inhibitor is compared with that of a compound known to affect the parameter to be evaluated. For example, a compound that is approved for use as an asthma control drug can be used. It will be appreciated that, if an active comparator is used as a control, an effective amount of a complement inhibitor may have less, more, or approximately the same effect as the active comparator at one or more points in time in different modalities.
[00358] [00358] In some embodiments, one or more biological effects of a complement inhibitor are evident when tested at various points in time during a dosage interval of the present invention, where such points in time cover at least 75% of the ingestion. - dosing range, for example, at least 80%, 85%, 90%, 95% or more of the dosing range. In some embodiments, one or more biological effects of a complement inhibitor are evident when tested at or near the end of the dosing interval, where "near the end of the dosing interval" means up to 2 days before the end of the dosing interval, for example, the day before the end of the dosing interval 5.
[00359] [00359] In some modalities, an animal model is used, for example, to help guide the selection of a dose, dose range or formulation for testing in humans, to evaluate one or more biological effects, etc. Animal models commonly used for airway inflammation and / or asthma involve inhaling the Ascaris suum antigen. For example, inhaling the Ascaris suum antigen by allergic monkeys (for example, the cynomolgus monkey; Macaca fasicularis) causes an initial bronchoconstriction and delayed allergic reaction, including a pulmonary inflammatory infiltrate. See, for example, Mellado, M., et al., J Pharmacol Exp Ther. (2008) 324 (2): 769-75; Zou, J., et al. Genome Biol. 2002; 3 (5): research0020. EPub 2002 Apr 11. Similar models exist in mice, sheep, guinea pigs, etc. In some embodiments, a significant reduction in allergen-induced EAR, LAR and / or AHR (for example, as assessed using methacholine) and / or a significant increase in CP (X) in treated animals compared to untreated animals indicates effectiveness. In some embodiments, a reduction in EAR, LAR and / or AHR remains evident at the end of a selected dosage range according to the present invention (for example, just before the next dose).
[00360] [00360] In general, adequate doses of complement inhibitor or other active agent, at least in part, depend on the potency of the complement inhibitor or other active agent, route of administration, etc. In general, dose ranges that are effective and well tolerated can be selected by an individual skilled in the art. Such do-
[00361] [00361] A potent compstatin analogue having the amino acid sequence of the compstatin analogs of SEQ ID NO: 28, was synthesized using standard methods. Soon, amino acids were obtained as Fmoc-protected amino acids, in which the -amine group of each amino acid was protected with Fmoc. Groups of side-chain functionals have also been blocked with several suitable protection groups. The synthesis was performed following the solid phase methodology, described by Merrifield (J. Amer. Chem. Soc. 85, 2149 (1963)). The assembly of the chain was carried out in the solid phase, with the conclusion that the N terminal was acetylated; the peptide was then cleaved from the solid phase and simultaneously deprotected via acidolysis using TFA and conversion to amide. The linear peptide was then oxidized and purified. A study was carried out to assess the efficacy of CA-28 after 14 days of administration in a non-human asthma primate model. In this study, a dose of 15 mg / kg of CA-28 in a 2.0% glycerol solution was administered to anesthetized animals (cynomolgus monkeys) via intratracheal nebulization once daily for 14 consecutive days, using a nebulizer pneumatic (Pari LC Plus, Pari USA, Midlothian, VA). Budesonide (10 mg / kg, administered once daily for 9 days as a powder using an insufflator), a glucocorticoid used to treat asthma, was used as a positive control. Major endings include the effects on bronchoalveolar lavage (BAL) cell counts, cytokine levels and changes in acute lung function, as assessed by airway resistance (RL) and dynamic compliance (CDYN) after challenges with Ascaris suum (A. suum).
[00362] [00362] The animals were submitted to the challenge with A. suum at 3 points in time (before the initial dose - Challenge), on day 14 (Challenge 1, that is, the last day of dosing) and on day 30 (Challenge 2 ). While each animal was anesthetized, a single dose of A. suum antigen was administered through intermittent positive pressure, breathing with a ventilator and in-line nebulization for more than 15 breaths. Each animal received an optimal response dose (ORD), which is the dose of the antigen (dilution) that historically caused an increase of> 40% in pulmonary resistance (RL) and a decrease of> 35% in dynamic conformity (CDYN) . Blood was collected by venipuncture and analyzed for routine clinical chemistry and hematology parameters.
[00363] [00363] Bronchoalveolar lavage (BAL) was performed by guiding a pediatric fiber optic bronchoscope past the carina to insert into a main bronchus. An attempt was made to wash different fields of the lung at each time point. Three washes of 5 sterile saline solution (20 mL each) were instilled and immediately aspirated for collection in the tubes. The first wash collection was placed in a 50 ml conical tube, while the second and third wash collections were combined in a second 50 ml conical tube. The samples were placed on wet ice or in a refrigerator to keep at 4ºC until transport. Cell pellets of different wash combinations (1st / 2nd / 3rd wash) were combined and analyzed for total and differential cell count. From stained slides, BAL and differential cell morphology were determined by counting a minimum of 200 nucleated cells from all washes (cell pellets were combined from all washes), if available, if less than 200 nucleated cells were available, this is documented in the study records and results. Relative and absolute counts were determined by macrophages, neutrophils, eosinophils, lymphocytes and mast cells. Erythrocytes, squamous epithelial cells and respiratory hair cells were not counted. BAL samples were analyzed by eotaxin, RANTES, IL-4, IL-5, IL-6, IL-8, IL-10, IL-13, IL-23, IL-17a and INF- using qualified methods. Results
[00364] [00364] After the A. suum antigen aerosol challenge during Challenge 0 (control challenge before dosing), all animals exhibited a severe bronchoconstrictor response, which was associated with increased pulmonary resistance (RL) and decreased dynamic compliance (CDYN) followed by pulmonary eosinophilia.
[00365] [00365] CA-28 did not affect the acute phase bronchoconstriction resulting
[00366] [00366] CA-28 resulted in a slight improvement (reduction) in eosinophilia after Challenges 1 and 2. However, eosinophil counts were higher in the reference samples collected after the dose and before the first challenge from A. suum.
[00367] [00367] Treatment with CA-28 inhaled at 15 mg / kg in a vehicle composed of 2.0% glycerol in water resulted in lower levels of most cytokines and chemokines increased compared to treated animals with vehicle control in the treatment session, in a way comparable to Budesonide in many cases, most notably eotaxin, IFN-y, IL-4, IL-13 and IL-23, although the suppression has not reached statistical significance in most cases, due to the high intrinsic variability of the data and the low number of animals. The most notable data were total suppression of IL-23 in animals treated with CA-28 seen at all points in time after Challenges 1 and 2. Inhibition of most other cytokines appeared to be present even after Challenge 2 in treated groups. with CA-28. CA-28 increased baseline levels of IL-10, key regulatory cytokines, after Challenges 1 and 2. Data are presented in graphical format in Figures 1-11.
[00368] [00368] The data are consistent with the conclusion that CA-28 creates an immune protective microenvironment (high IL-10, low IFN / IL-4 / IL-13 / IL-17 / IL-23) when the drug is present in the lung (Challenge 1) and 27 days after the drug's (washout) elimination (Challenge 2) (assuming a 1-day elimination for CA-28 and Budesonide). In particular, the levels of IL-17 and Il-23 in animals treated with CA-28 24 hours after Challenge 2 were lower than control animals, suggesting a sustained beneficial effect. In the case of Budesonide, the IL-17 / IL-23 axis appears to be increased 24 hours after Challenge 2.
[00369] [00369] Those skilled in the art will recognize or be able to verify using no more than routine experimentation, many equivalents for the specific modalities of the invention described here.
[00370] [00370] When the elements are presented as lists, it should be understood that each subgroup of the elements is also disclosed, and any element can be removed from the group. For the sake of brevity,
[00371] [00371] When intervals are given, terms are included. In addition, it should be understood that unless otherwise indicated, or otherwise evident from the context and understanding of the person skilled in the art, values that are expressed as intervals can assume any specific value or subscale within the scales declared in different modalities of the invention, to the tenth of the unit of the lower limit of the scale, unless the context clearly dictates otherwise. Any modality, aspect, element, resource, etc. of the present invention can be explicitly excluded from the claims. For example, any complement inhibitor, anti-Th117 agent, carrier, formulation, formulation component, disorder, subject population or characteristic (s), dosage intervals, route of administration or combination of these can be explicitly excluded. .

权利要求:
Claims (14)
[1]
1. Pharmaceutical composition, characterized by the fact that it comprises a complement inhibitor and an anti-Th17 agent.
[2]
2. Pharmaceutical composition, according to claim 1, characterized by the fact that the complement inhibitor inhibits C3 activity or C3 activation.
[3]
3. Pharmaceutical composition according to claim 1, characterized by the fact that the complement inhibitor comprises a compstatin analogue.
[4]
Pharmaceutical composition according to any one of claims 1 to 3, characterized in that the anti-Th17 agent comprises an antibody, small molecule, aptamer or polypeptide that binds to IL-1β, IL-6, IL-21, IL-22, IL-17 or IL-23 or binds to a receptor from any of the above.
[5]
5. Use of a complement inhibitor and anti-Th17 agent, characterized by the fact that it is in the preparation of a pharmaceutical composition for the treatment of a chronic complement-mediated disorder, a disorder associated with Th17, or for the interruption of a DC-Th17-B-Ab-C-DC cycle.
[6]
6. Use of a complement inhibitor, characterized by the fact that it is in the preparation of a pharmaceutical composition for the treatment of an individual who is suffering or is at risk of suffering from a complement-mediated disorder or a disorder associated with Th17, or for interrupting a DC-Th17-B-Ab-C-DC cycle.
[7]
7. Use of a complement inhibitor and an anti-Th17 agent, characterized by the fact that it is in the preparation of a pharmaceutical composition for the treatment of an individual who is suffering or is at risk of suffering from a mediated disorder by complement or a disorder associated with Th17.
[8]
8. Use according to any one of claims 5 to
7, characterized by the fact that the treatment comprises the administration of multiple doses of the composition to the subject according to the dosing schedule in which successive doses are administered, on average, (i) at least 2 weeks after the plasma concentration of the inhibitor the complement is reduced to no more than 20% of the maximum plasma concentration that was reached after the previous dose; (ii) at least two weeks after the plasma complement activation capacity has returned to at least 50% of the baseline value of the previous dose; (iii) at intervals equal to at least 2 times the terminal plasma half-life of the complement inhibitor; or (iv) at intervals of at least 3 weeks.
[9]
9. Use according to any one of claims 5 to 7, characterized by the fact that it comprises monitoring the subject for detecting evidence of a DC-Th17-B-Ab-C cycle and administering the composition to the subject based, at least partially, on a result of such monitoring.
[10]
10. Use according to any one of claims 5 to 7, characterized by the fact that it comprises monitoring the subject for the detection of a Th17 biomarker and administering the composition to the subject based, at least partially, on a result of such monitoring.
[11]
Use according to any one of claims 5 to 10, characterized in that the complement inhibitor comprises an antibody, aptamer, peptide, polypeptide or small molecule that binds to C3, C5, factor B or factor D .
[12]
Use according to any one of claims 5, 7, 8, 9, 10 or 11, characterized in that the anti-Th17 agent comprises an antibody, small molecule, aptamer or polypeptide that binds to IL- 1β, IL-6, IL-21, IL-22, IL-17 or IL-23 or binds to a receptor from any of the above.
[13]
13. Use of an anti-IL-23 agent, characterized by the fact that it is in the preparation of a pharmaceutical composition for the treatment of an individual who needs treatment for AMD.
[14]
14. Invention, in any form of its embodiments or in any applicable category of claim, for example, product or process or use encompassed by the material initially described, revealed or illustrated in the patent application.

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法律状态:
2020-11-24| B06F| Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]|

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2021-04-20| B07D| Technical examination (opinion) related to article 229 of industrial property law [chapter 7.4 patent gazette]|Free format text: DE ACORDO COM O ARTIGO 229-C DA LEI NO 10196/2001, QUE MODIFICOU A LEI NO 9279/96, A CONCESSAO DA PATENTE ESTA CONDICIONADA A ANUENCIA PREVIA DA ANVISA. CONSIDERANDO A APROVACAO DOS TERMOS DO PARECER NO 337/PGF/EA/2010, BEM COMO A PORTARIA INTERMINISTERIAL NO 1065 DE 24/05/2012, ENCAMINHA-SE O PRESENTE PEDIDO PARA AS PROVIDENCIAS CABIVEIS. |

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2021-06-15| B07E| Notification of approval relating to section 229 industrial property law [chapter 7.5 patent gazette]|

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2021-06-29| B06U| Preliminary requirement: requests with searches performed by other patent offices: procedure suspended [chapter 6.21 patent gazette]|

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2021-12-07| B350| Update of information on the portal [chapter 15.35 patent gazette]|

优先权:

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申请号 | 申请日 | 专利标题

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US201161499895P| true| 2011-06-22|2011-06-22|

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US61/499,895|2011-06-22|

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PCT/US2012/043845|WO2012178083A1|2011-06-22|2012-06-22|Methods of treating chronic disorders with complement inhibitors|

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