use of long-chain glycolipids as an agent with preservative properties against fungi, a method of in

专利摘要:
LONG CHAIN GLYCOLIPIDES USEFUL TO PREVENT MICROBIAL DETERIORATION OR CONTAMINATION OF MATERIALS. The present invention relates to the use of, and methods of use employing certain glycolipid compounds, as defined in detail below and having preservative or antimicrobial properties, to new compounds of the glycolipid class, and to the related modalities of the invention. The compounds have the formula (I), where m is (3) to (5), n is (2) to (5), o is (0) or (1) and p is (3) to (17), with the condition that the sum m + n + o + p is not less than (14); and R is a portion of carbohydrate linked by means of one of its carbon atoms to the binding oxygen, and / or a physiological, special and pharmaceutical or nutraceutical or cosmetically acceptable salt; or an ester thereof, as such or in the form of a composition, where the compound can be present in the form of an open chain and / or in the form of a lactone.
公开号:BR112013031371B1
申请号:R112013031371-4
申请日:2012-06-06
公开日:2020-08-25
发明作者:Marc Stadler；Jens Bitzer；Bärbel Köpcke；Kathrin REINHARDT；Jana Moldenhauer
申请人:Imd Natural Solutions Gmbh；
IPC主号:

专利说明:

[0001] [0001] The present invention relates to the use of, and methods of use that employ, certain glycolipid compounds, as defined in detail below and having preservative or antimicrobial properties, to new compounds of the glycolipid class, and to the modalities of related invention.
[0002] [0002] These modalities of the invention and related modalities of the invention are described below and in the claims, which are incorporated in the report described by reference. Background of the Invention
[0003] [0003] Bacteria and other microbial organisms cause food and beverage products, cosmetics and household care products, as well as other products to change, thereby reducing the validity or shelf life of such products or goods. In this way, several efforts have been made to reduce the harmful effects of microbial contaminants in food and beverage products, cosmetics, curative material and other materials, for example, medical devices, such as implants.
[0004] [0004] Other food preservatives, such as salt, sugar and vinegar, have been used for generations, and although relatively safe to use, their preservative effect is limited both in the duration of the effect and in the types of food and drink. for which they can be used. In addition, at higher levels, preservatives, such as salt and vinegar, can affect the taste of the product.
[0005] [0005] The preservatives commonly used for cosmetics include antimicrobial agents, such as quaternary ammonium compounds, alcohols, chlorinated phenols, parabens and paraben salts, imidazolidinyl urea, phenoxyethanol, p-hydroxybenzoate, small carboxylic acids, such as benzoic acid, sorbic acid, salicylic acid, formic acid, propionic acid or the corresponding salts. Formaldehyde releasers and isothiazolinones can also be used.
[0006] [0006] However, these materials may often not be tolerated or, for example, in the case of formaldehyde, they may even be toxic and even carcinogenic, or they may cause allergies or food intolerance.
[0007] [0007] Another preservative, for example, used in food and especially in drinks is sulfuric acid, although in meat products, for example, sausages, preserved meat and meat, stabilizers are often added that decrease water activity. , such as potassium and / or sodium nitrites and nitrates. Smoke is also often used to preserve meat products, with the undesirable side effect of forming polycyclic aromatic hydrocarbons that have carcinogenic properties.
[0008] [0008] Food and beverages have varying degrees of sensitivity to microbiological deterioration, depending on intrinsic factors of the food or drink, such as pH, nutrient content (for example, juice, vitamin, or micronutrient content) , the carbonation level, Brix (an indicator of sugar content), water quality (eg alkalinity and / or hardness), and preservatives.
[0009] [0009] Deterioration events occur when microorganisms are able to overcome the product's intrinsic protective factors and develop. The ability of microorganisms to overcome these barriers can be influenced, among other things, by the level of initial contamination, temperature, water content, for example, water activity, and packaging integrity. Recurrent contamination of cosmetics is also of particular importance, for example, by hand contact during normal use.
[0010] [00010] Several organisms are responsible for the deterioration of a variety of beverage materials, including cold-filled drinks. Yeasts such as Saccharomyces, Zygosaccharomyces, Candida, and Dekkera spp. are the most common. Also, acidophilic bacteria, such as Lactobacillus, Leuconostoc, Gluconobacter, and Zymomonas spp., And fungi, such as Penicillium, Aspergillus and Mucor spp., Can deteriorate various materials that contain water.
[0011] [00011] Other materials and also other types of drinks are susceptible to deterioration by microorganisms. The spores of thermophilic, acidophilic bacteria, such as Alicyclobacillus spp., And the heat-resistant fungi spores of Byssochlamys, their anamorphic (asexual) stages Paecilomyces, and Neosartorya spp. they can survive pasteurization and can deteriorate hot, non-gaseous products, such as sports drinks and teas. Also, the packaged waters are susceptible to contamination by fungi.
[0012] [00012] In cosmetic products, for personal care and home care, deterioration occurs by a variety of microorganisms, ranging from Gram-positive bacteria (eg Staphylococcus spp.), Gram-negative bacteria (eg Escherichia coli, Pseudomonas spp.) To yeasts (for example, Candida albicans) and common fungi (for example, Aspergillus niger). The microbial growth in these, or on these, products depends on several intrinsic factors, such as the water activity of the formulation (the minimum water activity requirements for growth or proliferation range from 0.99 for the Acinetobacter species to 0, 61 for some fungal species), the composition of the formulation, the pH value (for example, the optimum pH for the growth of most yeasts and fungi is between 4.0 and 6.0), and the processing conditions, such as temperature. Although high temperatures, for example, 80 ° C for 20 minutes, can reduce microbial contamination during processing, it is important to prevent inactivation or degradation of preservatives in the formulation. In addition, the packaging of the product, the solubility of the preservative and its antimicrobial susceptibility profile will influence the conservation effectiveness and, consequently, the shelf life of the products.
[0013] [00013] Protection against microbiological deterioration of sensitive products can be achieved using chemical preservatives and / or processing techniques, such as hot insertion, tunnel pasteurization, ultra-high temperature (UHT), or pasteurization followed by aseptic packaging, and / or pasteurization followed by cooling the drink. In general, beverages with a pH <4.6 can be chemically preserved, processed hot, and placed in packaging in such a way that the product is not contaminated again. For example, process techniques, such as cold filling, can be used to store a cold filled beverage. In a similar way, this same drink can be processed using non-preserved techniques, such as hot filling, tunnel pasteurization, pasteurization followed by aseptic filling, or requiring the drink to be cooled, that is, under refrigeration, after the pasteurization stage. Drinks having a pH 2-4.6 should be processed in such a way that the spores are destroyed using ultra-high temperatures, followed by aseptic filling in packaging or using a retort.
[0014] [00014] Current preservation systems for acidic, shelf-stable, aerated and non-aerated food or beverages, for example, soft drinks, generally use weak acid preservatives (eg benzoic and / or sorbic acid). Benzoic and sorbic acids (and their salts) effectively inhibit yeasts, bacteria and fungi, with a few exceptions. Weak acids in drinks exist in balance between their dissociated and non-dissociated forms, which is dependent on the acid dissociation constant (pKa) and the pH of the drink. The pKa for benzoic acid is 4.19 and the pKa for sorbic acid is 4.76. A pH of the drink below the pKa of the acid involved pushes the balance into the undissociated form. The non-dissociated form is more effective against microorganisms; therefore, weak acid preservatives are most effective in the low pH range.
[0015] [00015] The preservative properties of weak acids can be increased by adding preservative enhancers, such as chelating compounds, to the material to be preserved, for example, a food, drink or cosmetic preparation. For example, common added chelating compounds include disodium calcium ethylene diaminetetraacetic acid (EDTA) or one or more of the polyphosphates, such as sodium hexametaphosphate (SHMP).
[0016] [00016] In non-gaseous products, with high nutrient content, such as drinks containing juice, vitamins, and / or minerals, weak acids are more likely to exert inhibition if used in conjunction with preservative enhancers. However, weak acid conservation systems, however, have limitations:
[0017] [00017] Genetic adaptation and subsequent resistance by microorganisms is a major concern (see Piper. Et al., Microbiol. (2001) 147: 2635-2642). Certain yeasts, such as Z. bailii, Z. bisporus, Candida krusei, and S. cerevisiae, have specific genes that enable them to resist weak acid preservatives and grow. This happens despite the presence of preservatives and regardless of the presence together of EDTA or SHMP. It is also believed that some bacteria, such as Gluconobacter spp., Are resistant to preservatives. It has been shown that the levels of weak acids needed to overcome this resistance are well beyond the regulatory limits on levels of use. Most often, the deterioration of preserved teas, drinks containing juices, and fizzy drinks is due to preservative-resistant microorganisms.
[0018] [00018] Medium chain saturated fatty acids and their esters with oligohydroxylated compounds have been found to have inhibitory effects against various bacteria and fungi. The minimum inhibitory concentration values reach a maximum with a chain length of about 8 to 12 carbon atoms (Varvaresou, Int. J. Cosmetic Sci (2009) 31: 163-75).
[0019] [00019] In addition, the other process techniques for low acid drinks (ie, pH> 4.6) have limitations. Such low acid beverages must be heat treated, sufficiently to destroy the spores of the species Clostridium botulinum and Bacillus (B. cereus, B. subtilis and others). Examples of such processes include UHT and retort. Even after such processing, beverage products must be handled in a way to prevent contamination after processing. The research, however, suggests that there may still be several strains of microorganisms that can survive these different processing techniques. To this end, these processing techniques may not eliminate the potential for deterioration.
[0020] [00020] Other chemical preservatives can also cause adverse side effects when consumed. Thus, many existing preservatives must be regulated and have upper limits legally set for use. In addition, many preservatives, such as sodium benzoate, propionates, aromatic benzenes, organic acids, propylene glycol and glycerol, for example, when used in sufficient levels for antimicrobial effects, give the drink a bad taste. or to the food, masking or altering to some degree the taste expected by the consumer. Weak acids can cause a burning sensation in the throat or mouth when used at high levels. Although there are certain stable drinks on the shelf where this quality may be acceptable, this sensory perception is often considered to be negative. Similarly, polyphosphates used in weak acid conservation systems may have some limitations. For example, polyphosphates can give a drink a bad taste.
[0021] [00021] Certain emollient solvents exhibit synergistic action when combined with essential oils or ingredients against microorganisms, as noted in WO 03/034994, which is incorporated by reference in its entirety. Emollient solvents used as preservatives in cosmetics do not normally produce skin reactions and, in addition, make the skin smooth and silky.
[0022] [00022] In a series of publications, Nishida et al. described compounds from an unidentified strain of "Basidiomycetes sp." to produce glycolipids, which are supposed to exhibit inhibitory activity against Gram-positive bacteria (Nishida, Tetrahedron Lett 1988, 29 (41): 5287-90; Chem Pharm Bull 1990, 38 (9): 2381-9; J Antibiot 1991, 44 (5): 541-5; Chem Pharm Bull 1991,39 (11): 3044-7; Proc "Symposium on the chemistry of natural organic compounds" 1987, 29: 729-36; ibid 1990, 32: 253-9 ) and against infection by the polio and herpes viruses (J Chrom 1994, 664 (2): 195-202; J Mass Spectrom Soc Jpn 1995, 43 (1): 27-36; ibid 37-44). Much effort was made to elucidate the structure and the results were presented in detail in the publications cited above; however, the authors did not provide any data on the suggested antimicrobial activity. Then, one of the compounds isolated by Nishida et al. (Glycine IVA) has been described as an antifungal agent from a Dacrymyces sp. (Wunder, A., Diss. 1995, Univ. Kaiserslautern, Germany). Mierau (Z Naturforsch 2003; 58c: 541-6) made reference to this work. In none of the publications cited were details described about the biological data and the identity of the producing organisms.
[0023] [00023] JP 2006-176438 A and J. Antibiot. 2007, 60, 633-639 disclose F-19848 A, a glycolipid obtained from the fermentation broth of the fungus strain Dacrymyces sp. SANK 20204, as an inhibitor of the CD44 hyaluronic acid binding receptor and as being useful to treat or prevent degenerative arthritis or a disease caused by degenerative arthritis.
[0024] [00024] Biotensive agents are produced extracellularly or as a part of the cell membrane by various organisms, such as bacteria and fungi. Their structures usually contain a hydrophobic nonpolar portion that consists of unsaturated, saturated, and / or oxidized lipids or fatty acids, and a hydrophilic component, which may be composed of amino acids, carbohydrates, phosphates or cyclic peptides. They are generally classified into glycolipids, lipopeptides, phospholipids, fatty acids and polymeric compounds according to their chemical structures. Biotensive agents are produced by a wide variety of microorganisms and, therefore, differ in their chemical structure. Some biotensoactives have antimicrobial activity against bacteria, yeasts, fungi or viruses. In addition, they can prevent microbial colonization of surfaces, such as those from implanted medical devices, through their ability to disrupt biofilms on these surfaces.
[0025] [00025] Soforolipids, ramnolipids and mannoyl erythritol lipids are the most widely used glycolipid biotactives in cosmetics.
[0026] [00026] Ramnolipids are known for their efficiency in removing nosocomial microbes in biofilms. A biofilm is characterized by a strong adhesion activity of the attached microorganisms. The known biotensoatives with anti-adhesive or biofilm disrupting activity are produced by Lactobacillus acidophilus, L. fermentum, Lactococcus lactis, Streptococcus thermophilus, Bacillus subtilis, B. licheniformis, Brevibacterium aureum, Pseudomonas aeruginosa, and P. put.
[0027] [00027] The genus Lactobacillus produces the lipopeptide surlactin, the genus Bacillus produces lipopeptides that belong to the families of secondary metabolites like fengicin and type surfactin. Another lipopeptide was isolated from Brevibacterium aureum. Streptococcus thermophilus is a producer of glycolipids not yet identified, which contain considerable amounts of nitrogen (Rodrigues, Colloids & Surfaces B: Biointerfaces (2006) 53: 105-112). Additional known producers of biotensoactives that exhibit activity against microorganisms involved in biofilms are Pseudomonas aeruginosa, which produces ramnolipids, and P. putida, which produces lipopeptides. Nitrogen-containing biotensives produced by Streptococcus mitis have not yet been identified. Lactococcus lactis produces a low molecular weight [467 Da] biotensoactive that consists of methyl-2-O-methyl-beta-d-xylopyranoside with octadecanoic acid. (Saravanakumari and Mani, Bioresour Technol (2010) 101: 8851-8854).
[0028] [00028] A good overview of the different classes of known biotensoatives is given by Rahman et al. (Biotechnology (2008): 360-70) showing great advantages of these compounds against synthetic compounds, such as less toxicity, greater biodegradability, better environmental compatibility and their ability to tolerate extreme temperature, pH and salinity. However, the difficulties described in production teach the limitation of industrial use as being the low relative yields, the high fermentation costs and the difficult procedures of isolation within the industrial processes. The economically acceptable production of biotensoactives on a large scale is suggested by the use of industrial waste as a process medium.
[0029] [00029] As commonly understood in the art, the definitions of the terms "preserve", "preservative" and "conservation" do not provide a standard period of time for how long the material to be preserved is preserved from deterioration, decomposition, or discoloration . The time frame for "conservation" can vary widely, depending on the subject matter. Without an established time frame, it may be difficult or impossible to deduct the time frame required for a composition to act as a "preservative".
[0030] [00030] In summary, many preservatives and preservation methods have undesirable side effects, such as toxicity, allergenicity, carcinogenicity, occasionally resistance formation, and / or are often not accepted by consumers at a time when natural conservation is preferred over conservation with synthetic products or other products that have a negative image for health.
[0031] [00031] Consequently, there is a great need for naturally derived, non-toxic, relatively inexpensive, effective preservative compositions that avoid the disadvantages as mentioned and are able to reduce microbial contamination and simultaneous deterioration in a wide variety of food, drinks, cosmetics, and other perishable consumer products, but without appreciably altering the taste, color, odor, or function of the product. General Description of the Invention
[0032] [00032] Surprisingly, new compounds have been found and isolated from strains of Dacryopinax spathularia and other fungal strains belonging to the family of Dacrymycetaceae, and for them, as well as for compounds of the same class, they have been found to have new and useful properties . The compounds are described in more detail below.
[0033] [00033] The class of compounds of formula I, both as they are not new, as they are new, is not known in the art for any activity that prevents deterioration / damage.
[0034] [00034] Very surprisingly, therefore, it has been found that these compounds exhibit a strong inhibitory activity against microorganisms that are responsible for the damage or deterioration of orally consumable products (such as food products and drinks) or cosmetic compositions . For example, a Dacryopinax extract according to the present invention can show a broader spectrum of activity than the corresponding individual components of the produced glycolipid complex (glycolipid mixture) that have been isolated to purity. Such multi-component mixtures exhibit remarkable long-term activity against several important deteriorating microbes, including, for example, the species Zygosaccharomyces and Bacillus.
[0035] [00035] More surprisingly, the microorganisms used in the present invention make it possible to produce large quantities of the compounds of formula I in a cost-effective production process.
[0036] [00036] They are, for example, capable of reducing the growth of microbial contaminants, such as bacteria, yeasts, fungi and other microorganisms and their spores, especially those that are resistant to temperature, for example, thermophilic , or heat-resistant, or acidophilic, for example, microorganisms that tolerate a lower pH value that cause deterioration of food, drinks, cosmetics and other materials.
[0037] [00037] Glycolipids do not exhibit a characteristic taste or an unpleasant sensation in the mouth and, therefore, this application refers to the use of glycolipids in materials that come into contact with the oral cavity of a human being. This application also refers to the synergistic combinations of antimicrobial ingredients that can be used in orally consumable compositions, such as food and drinks, without imparting bad flavors.
[0038] [00038] Still surprisingly, it was found that the use of a simple medium, which uses only dextrose or glucose as a carbon source and a small amount of yeast extract, can be superior against the typical complex media, known from the literature . However, the use of other growth media or substrates is also included. In addition, it was found that this culture medium endured particularly high production rates in a relatively low biomass production, which facilitated the downstream processing of crude glycolipid products, allowing their easy recovery from the culture fluid by precipitation.
[0039] [00039] The glycolipids of the present invention can be shown to demonstrate a broad antimicrobial spectrum and can be incorporated as additives in various materials, such as a preservative or an agent with preservative activity, especially as a cosmetic additive and / or food additive and / or additive for drinks based on this antimicrobial activity.
[0040] [00040] Although the mechanism of action for glycolipids is unknown, these compounds can, without being intended to mean a comprehensive and final definition of their properties, be considered as biotensoactive and, in addition, they can influence the cell membranes of microorganisms . The other known biotensoactives are ramnolipids, soforolipids, lipopeptides, such as chlamydocin, surfactin, lichenisin G, etc., as cited by Mukherjee (in "Biosurfactins", R Sen ed., Springer, 2010, chapter 4, "Microbial Surfactants and Their Potential Applications"). The cited biotensoatives differ from the compounds of formula I of the present invention. Although Mukherjee listed compounds specified as glycolipids, the structures of these compounds differ significantly from those of the present invention:
[0041] [00041] The compounds useful according to the invention are, in general, characterized a long-chain fatty acid with at least 20 carbon atoms; a portion of carbohydrate that is not linked to the acid group; by an alpha hydroxyl group; and by at least one additional hydroxyl group, at the "center" of the fatty acid carbon chain, clearly separated from both the alpha position and the glycosyl substituent.
[0042] [00042] Especially the compounds of the formula I represented below, which are esterified in their carbohydrate portion by acids other than acetic acid, in particular esterified by isovaleric acid, are not known in the art and, therefore, are new. Detailed Description of the Invention
[0043] [00043] In a first embodiment, the invention relates to the use of a compound of formula I, or a mixture of two or more such compounds of formula I,
[0044] [00044] Use is preferred where in the compound of the formula I m is 3 to 5, n is 2 to 5, o is 0 or 1, p is 5 to 15 and R is a portion of the sub-formula
[0045] [00045] In other terms: A compound for use according to the invention is especially a linear carboxylic acid with at least 20 carbon atoms, preferably 22 to 28, 24 to 26, in particular 26, substituted in position 2, which means the alpha position, with a hydroxyl group; replaced with a second hydroxyl group in the omega-5, omega-6 or omega-7 position, which is itself replaced by a carbohydrate, for example, as defined below, and an additional hydroxyl group (a third) among the omega substituents and those in alpha, which is separated from the second by two to five methylene groups, where optionally this third hydroxyl group has a vicinal hydroxyl group, towards the acidic end.
[0046] [00046] In another embodiment of the invention, the compound or compounds of formula I, a physiologically acceptable salt thereof, and / or an ester thereof, are added in the form of an extract from a natural source or obtained from such an extract. Preferably, the source of the extract is a fungus that belongs to the family Dacrymycetaceae, a species of the genera Dacryopinax, Ditiola, Guepiniopsis and / or Femsjonia, more especially Dacryopinax spathularia, Dacrymyces sp., Dacrymyces stillatus, Dacrymyces chrysocomus or Gacrypsis chinos Femsjonia luteo-alba (= Ditiola pezizaeformis). Particularly preferred are the Dacryopinax spathularia MUCL 53181 strain, the Dacryopinax spathularia MUCL 53182 strain, the Ditiola radicata MUCL 53180 strain, the Mucl 53179 nude Ditiola strain of the chrysocomus CBS2 chrysocomus c.82 alba (= Ditiola pezizaeformis) MUCL 53500.
[0047] [00047] In our investigations, it appears that the strain of Dacryopinax spathularia MUCL 53181 is the best strain identified so far to produce compounds of formula I and mixtures of two or more compounds of formula I, in particular those described in detail next, particularly the compounds of formula I that exhibit the strongest antimicrobial activity against yeasts and fungi. Thus, in a further aspect, the present invention also relates to the strain of Dacryopinax spathularia MUCL 53181 as such.
[0048] [00048] Another modality refers to the use as described above or below one or more compounds of formula I, where the material to which such compound (s) is (are) applied is subjected to a treatment thermal energy before, during or after the addition of the compound (s) of the formula I, a physiologically acceptable salt thereof and / or an ester thereof.
[0049] [00049] In another embodiment, the invention relates to a new compound of formula I, or a mixture of two or more compounds of formula I including a new compound of formula I, where the compound or compounds may be present in the form open-chain and / or in the form of a lactone, and / or a pharmaceutical or nutraceutical or cosmetically acceptable salt thereof, as such.
[0050] [00050] Another embodiment of the invention relates to a compound or mixture of compounds of the formula I shown above or as defined above or below, where the R portion carries at least one hydroxyl group esterified with an acid having 3 or more atoms carbon, a physiologically acceptable salt, and / or an ester thereof, especially where the acid is a C3-C10-alkanoic acid, especially isovaleric acid; a physiologically acceptable salt, and / or an ester thereof, more especially a compound selected from the group of compounds represented by the following formulas:
[0051] [00051] In yet another embodiment, the present invention relates to a preservative or antimicrobial composition, comprising as an active agent a compound or a mixture of compounds of formula I, a physiologically acceptable salt thereof, and / or an ester thereof, as shown or defined in any one of claims 1,3, 4 and 8 to 15, alone or with another additive, such as a carrier material, where the preservative composition is especially for use in a cosmetic, a food, a drink, a substance pharmaceutical, a medical device, or an active packaging material, especially in the form of a powder or liquid, for example, a composition that is a coating or film. The composition, in a more specific embodiment of the invention, can be a precursor to a drink, especially a concentrate, syrup or powder.
[0052] [00052] In another embodiment, the composition defined in the preceding paragraph is an antimicrobial composition to increase stability against microorganisms, especially where at least one microorganism is selected from the group consisting of fungus, yeast and bacteria.
[0053] [00053] In another embodiment, the composition according to either of the preceding two paragraphs is a preservative or antimicrobial composition for a pharmaceutical substance, a medical device, a food container, a beverage container, or especially a food, beverage , a cosmetic, or a home care product.
[0054] [00054] In another embodiment, the composition according to either of the preceding two paragraphs is a biofilm inhibiting agent and is used as such by administration, or in methods that comprise the administration, of one or more compounds of formula I, or from a composition that comprises them, to surfaces or materials that come into contact with the surface. In this way, biofilms on different materials can be avoided, including medical devices, teeth, containers, household care products, pipes or the electrical network or other devices that conduct or contain liquids and the like.
[0055] [00055] In yet another embodiment of the invention, the composition according to any of the preceding four paragraphs comprises an additional preservative.
[0056] [00056] The invention, in yet another embodiment, also relates to an extract comprising one or more compounds of formula I, a physiologically acceptable salt, and / or an ester thereof, as shown or defined above or below.
[0057] [00057] A further embodiment of the invention relates to a method of increasing the microbial stability of a material, comprising adding to said material one or more compounds of formula I, a physiologically acceptable salt, and / or an ester thereof, as shown or defined above or below, preferably a material selected from the group consisting of a cosmetic, a food, a drink, a pharmaceutical substance, a household care substance, a medical device, and an active packaging material, especially a drink, or a food, or a cosmetic.
[0058] [00058] Another embodiment of the invention relates to a material comprising, as or within a coating and / or as a mixture, an additive in the form of a compound or a mixture of compounds of formula I, a physiologically acceptable salt thereof and / or an ester thereof, as defined above or below. This material must be different from the fungus from which the compound or compounds of formula I are extracted. In another embodiment of the invention, the material is a cosmetic, a food, a drink, a pharmaceutical substance, a household care substance, a medical device, or an active packaging material, especially a beverage, a beverage precursor, especially a concentrate, syrup or powder, a food or a cosmetic. In another embodiment, such material comprises an additional preservative.
[0059] [00059] Another embodiment of the invention relates to a material according to the preceding paragraph, which is obtained after heat treatment.
[0060] [00060] The invention also relates to a method of extracting and / or isolating one or more compounds of formula I, especially as described below and / or in the Examples.
[0061] [00061] The invention also relates to the modalities in the claims and especially in the dependent claims which are incorporated by reference in the description together.
[0062] [00062] The compounds of the present invention or useful according to the present invention of formula I, according to the present understanding, are produced only by fungi of the family Dacrymycetaceae, particularly by fungi of the genera Dacryopinax (for example, D. spathularia) , Dacrymyces, Ditiola (for example, Ditiola radicata or Ditiola nuda), Guepiniopsis and Femsjonia (for example, F. luteo-alba). All of these fungi have in common that they belong to the family Dacrymycetaceae. All species of Dacrymycetaceae hitherto known are saprotrophs that live on wood, which can cause brown rot or white rot (Seifert, Mycologia 75 (1983): 1011-1018). Although they can be easily isolated from basidiocarp spores that grow in the field and readily grow in the crop, relatively few strains of Dacrymycetes are deposited in public collections.
[0063] [00063] The fungal genus Dacryopinax GW Martin was instituted by Martin (Lloydia 11 (1948): 111-122) and currently comprises 23 accepted taxa, including 22 species and a variety (see Mycobank; http://www.mycobank.org ). According to the latest database, the genus is currently classified in Basidiomycota, class Dacrymycetes, order Dacrymycetales, family Dacrymycetaceae. The members of the Dacrymycetes (formerly often referred to as Dacrymycetales order, before being elevated to the class category) are characterized by their unique basidial morphology, with two equidiameters of epibasid, thus modeling the basidium as a tuning fork. In addition, they have dolipores with continuous parentessomes.
[0064] [00064] These common morphological and ultrastructural characteristics have been supported very well in several molecular phylogenetic studies, as reflected by Hibbett (Mycologia 98, 917-925, 2006) and by the references cited in it. Most species of Dacrymycetes still belong to the order Dacrymycetales, family Dacrymycetaceae. This family comprises eight genera, which have traditionally been separated according to macroscopic morphological characters (mainly referring to the habit of basidiocarps) and microscopic (for example, the thickness of the wall of marginal hyphae in the sterile parts of the basidiocarps) of the fruiting bodies (that is, the basidiocarps). However, this classification is not clear and, therefore, has given rise to several alternative taxonomic concepts during the past decades. For example, McNabb (N.Z.J. Bot 3, 59-72, 1965) gave the first complete treatment of Dacryopinax and limited his basidiocarp habit as follows; "Extremely variable fructifications in shape, stipitate with a spathulate, petaloid, flabellate, cupulate, obliquely cupulate, inversely cupulate, foliose, or occasionally lobed and somewhat morchelloid pileus" ("Extremely variable fruiting in shape, with stipe having a spiked, petaloid cap , flabby, domed, obliquely domed, inversely domed, folios, or occasionally lobulated and somewhat morchelloide "). This limitation, which is still valid today, suggests that Dacryopinax is a complex genus. In addition, recent molecular phylogenetic studies by Shirouzu et al. (Mycoscience 48: 388-394, 2007 and Persoonia 23, 16-34, 2009) suggested that converging evolutionary developments in Dacrymycetales and Dacrymycetaceae could have given rise to the development of similar morphological characteristics, for this reason the basidiocarps that are characteristic of Dacryopinax and other genera of Dacrymycetaceae could have developed independently more than once.
[0065] [00065] The species of the currently accepted type of Dacryopinax is Dacryopinax elegans. However, without a doubt, the species most cited in the literature is Dacryopinax spathularia (Schwein Fr.) G.W.Martin. This species was first described from South Carolina, USA, and was treated under different names (ie Merulius spathularius, Guepinia spathularia) before Martin proposed the genus Dacryopinax. Under Guepinia spathularia (which is a later, invalid synonym for a plant genus name and therefore had to be abandoned), this fungus has been described to occur in several tropical and subtropical regions of the world, including North Australia, New Zealand, Asia and America, by Saccardo, PA, Sylloge Fungorum 6, p 808 (1888) and has since been described from numerous other countries in the world. Dacryopinax spathularia has an unusual geographical distribution. According to McNabb (N.Z.J. Bot 3 (1965): 59-72), it is widely distributed across both hemispheres, but has never been found in Europe, although it occurs in North Africa and eastern Russia. The species is characterized by having variable basidiocarps, although typically spatulated, up to 2.5 mm in height, spores from a single septum, and by the ab-hymenials, with thick walls.
[0066] [00066] Dacryopinax spathularia is one of the species in the family and in the order that it is capable of producing comparably large basidiocarps. It has been reported to be used as an "edible mushroom" by the indigenous population of the Republic of Cameroon (Van Dyck et al., Ambio 32 (2003): 19-23). The entire fungal family Dacrymycetaceae does not contain any poisonous species, although the basidiocarps of most species are somewhat inconspicuous and / or have a hard, rubbery consistency that prevents their culinary use. Interestingly, cultures of certain Dacrymyces species, which can be considered as closely related to Dacryopinax, have been patented for their usefulness in the production of carotene pigments (US Patent 2,974,044). Carotenoids are also apparently the only secondary metabolites that have so far been described from a species of Dacryopinax, and their production in D. spathularia cultures has been studied in detail by Vail and Lilly (Mycologia 60 (1968): 902-907).
[0067] [00067] The fungal strains that produce the compounds of the present invention were characterized by morphological methodology, using phase contrast microscopy of cultures grown on solid YMG medium, and by molecular phylogenetic methods. As the nucrDNA LSU or 28S / 5.8S was recently described to be informative for the phylogenetic assessments of Dacrymycetes by Shirouzu et al (Persoonia 23, 16-34, 2009), and the authors of this work published several reliable reference sequence data, this DNA region was chosen for comparison in the characterization of the strains of Dacrymycetes that are the subject of the present invention.
[0068] [00068] DNA for PCR has been isolated from YMG cultures. The LSU or 28S / 5.8S nucrDNA regions were then amplified using the LR7 and 5.8SR primers (Vilgalys Lab, Duke University, Durham, USA, http://www.biology.duke.edu/fungi/mycolab/primers.htm ), using the PCR PCR Taq Core Kit (Qiagen, Hilden), and applying a standard thermal profile with an annealing temperature of 53 ° C. The amplification products were purified using SigmaSpin Post-Reaction Clean-Up columns (Sigma-Aldrich), using the protocol provided by the manufacturer. The nucleotide sequences were obtained by cycle sequencing using a DNA Cycle Sequencing Kit (Jena Bioscience, Jena, Germany) and the 5 ′ IRD700 LR0R primer (Vilgalys Lab). The marked primers were synthesized to order by Eurofins MWG Operon, Ebersberg, Germany. The cycle sequencing products were then analyzed using a LI-COR 4200 genetic analyzer (Li-Cor Bioscience, Lincoln, NB). In what follows, the characteristics of the five strains that have been identified from producers of the glycolipids that are the subject of the present invention are briefly summarized.
[0069] [00069] The FU50088 strain was isolated from the sporocarp of an unidentified basidiomycete growing on wood, in French Guiana, by Sergej Buchet in 2002, supplied to Bayer Healthcare AG, and selected for fermentation to provide extracts that are suitable for the examination of natural products. On YMG agar, at 23 ° C, the culture reached about 10 mm in diameter after 10 days of incubation. The mycelium at first appeared velvety and white, but soon it became a strong yellowish color. The occasional presence of fixation links revealed that the fungus belongs to Basidiomycota. After 5 days of incubation, conidiogenous cells appeared in abundance over the vegetative hyphae, showing sympodial, poliblastic conidiogenesis, producing subglobous hyaline conidia, averaging 5-6 x 2.5-3 μm in size. These characteristics were found to be basically in agreement with the data described by Shirouzu et al (Persoonia 23, 16-34, 2009). The LSU nucrDNA sequence of this strain FU50088 is included here as the <SEQ ID NO: 1> sequence.
[0070] [00070] The strain was studied in comparison with an authentic strain of Dacryopinax spathularia, CBS 197.63, originating from Africa, which was obtained from Centrallbureau voor Schimmelcultures, Utrecht, Netherlands. Its morphological characteristics, as well as its production of secondary metabolites were largely in agreement with that of the FU50088 strain. In addition, a high degree of homology was observed between the 5.8S / ITS nrDNA and 28S nrDNA sequences of the two strains mentioned above and the reference DNA sequence data that had been published on the Internet by experts in the taxonomy and phylogeny of Basidiomycota , under the name of Dacryopinax spathularia or its synonyms. Therefore, the strain FU50088 was identified to belong to the species Dacryopinax spathularia by the phylogenetic and chemotaxonomic molecular, morphological methodology, and is referred to in this document as this species.
[0071] [00071] The reference strain Dacryopinax spathularia CBS 197.63, collected from Bangui, Central African Republic, isolated by J. Boidin and deposited with CBS in April 1963, was similar to the strain of Dacryopinax spathularia FU50088 in its growth and morphological characteristics . However, their pigmentation was not as intense, and even in aged cultures, mycelia only turned light yellow. Conidia were subglobose to ovoid, measuring 3-6.5 (-8) x 2.5-4 μm. The LSU nucrDNA sequence of this CBS 197.63 reference strain is included here as the <SEQ ID NO: 2> sequence.
[0072] [00072] Three other strains that were not specified for the genus Dacryopinax, but are also members of the family Dacrymycetaceae, were obtained from collections of public cultures, studied and verified to produce the compounds of the invention. Its history and characteristics are given below:
[0073] [00073] The Ditiola nuda strain CBS 173.60 was isolated in Shirokane, Tokyo, Japan, from a petiole of the Shiia sieboldii plant according to the information provided in the CBS catalog and deposited with CBS by K. Tubaki in 1960. The strain showed growth characteristics similar to Dacryopinax spathularia FU50088 and like those of this strain; their conidia measured 6-5 x 2.5-3 µm. The LSU nucrDNA sequence of the CBS 173.60 Ditiola nuda strain is included here as the <SEQ ID NO: 3> sequence.
[0074] [00074] The strain Ditiola radicata CBS 126.84 was isolated from sporocarps growing on wood with gymnosperm collected in August 1982 in Canada, Alberta, Banff National Park, C Level Cirque Trail, by Keith A. Seifert and deposited with CBS in 1984. The strain showed similar growth and morphological characteristics to Dacryopinax spathularia FU50088, but had smaller conidia (4-5 x 1.5-2 pm). The LSU nucrDNA sequence of the strain Ditiola radicata CBS 126.84 is included here as the <SEQ ID NO: 4> sequence.
[0075] [00075] The strain Ditiola pezizaeformis ATCC13299 was originally deposited with ATCC as Femsjonia luteo-alba. The strain had been used in a U.S. patent application US 2,974,044 and claimed to be a carotenoid producer. However, according to current taxonomy, Femsjonia luteo-alba is a synonym for the internationally accepted, valid name, Ditiola pezizaeformis, as described by Reid (A monograph of the British Dacrymycetales. Transactions of the British Mycological Society 62 (1974): 433 -494) and in accordance with current entries on Mycobank and other Basidiomycetes taxonomic databases and monographs. The ATCC13299 strain also showed growth and morphological characteristics similar to the FU50088 strain, and its conidia were elongated-ellipsoid to subglobous, 5-6.5 x 1.5-2 µm. The LSU nucrDNA sequence of the strain Ditiola pezizaeformis ATCC13299 is included here as the <SEQ ID NO: 5> sequence.
[0076] [00076] The Dacryopinax spathularia FU50088 strain was deposited under the Budapest Treaty at BCCM / MUCL, Mycothèque de l'Université catholique de Louvain, Place Croix du Sud 3, B-1348 Louvain-la-Neuve, Belgium, under the number of designation MUCL 53181, on 11.10.2010.
[0077] [00077] The other strains that produce the compounds of the invention were also deposited under the Budapest Treaty in BCCM / MUCL: Dacryopinax spathularia, CBS 197.63 under the designation number MUCL 53182, on 11.10.2010, Ditiola radicata, CBS 126.84 under MUCL designation number 53180, on 11.10.2010, Ditiola nuda, CBS 173.60 under the designation number MUCL 53179, on 11.10.2010, and Femsjonia luteo-alba Fr.1849, ATCC13299 under the designation number MUCL 53500, on 19.05. 2011.
[0078] [00078] Surprisingly, the compounds of the invention, for example, the compounds of formula I, exhibit a long-lasting, strong inhibitory activity against organisms involved in the deterioration of pharmaceutical, nutraceutical, nutritional, cosmeceutical, and / or preparations cosmetics. Said compounds are especially useful against acidophilic spoilage yeasts, which are involved in the damage or spoilage of drinks. Even more surprisingly, these compounds are able to inhibit the growth of thermophilic fungi, which are difficult to control with standard sterilization and / or pasteurization processes. Brief Description of Drawings
[0079] [00079] Figure 1: Numbering of the atoms of the compounds of the invention for assigning signals from analytical processes.
[0080] [00080] Figure 2: HPLC-MS typical of an extract of FU50088 (MUCL 53181) produced following the Fermentation of example 1 B c) 2001 of fermentation; a Preparation C of the extracts c) Preparation of a sedimentation product. Adjust the signals according to the "Adapted Method" of Example 3. The numbers in brackets "[..]" represent the corresponding compound in Table 1.
[0081] [00081] Figure 3: HPLC-ELSD chromatogram typical of an extract of the strain of Ditiola pezizaeformis ATCC13299 (MUCL 53500), notation of the peak according to Table 16 below.
[0082] [00082] Figure 4: HPLC-MS typical of an extract [X8] obtained from the strain Dacryopinax spathularia FU50088 (MUCL 53181) following example 8 D), annotating the signals according to the "improved method" of Example 8 C ) for details, see Table 24A below. Applications and definitions
[0083] [00083] The general expressions, within the present description, preferably have the following meaning, where, in each modality, one, more than one or all of the more general expressions can, independently of each other, be replaced by more specific definitions , thus forming the special, for example, preferred embodiments of the invention, respectively:
[0084] [00084] Preferably, the compounds of formula I are natural compounds, that is, compounds that are present in, and can be isolated from or extracted from, natural sources (especially those mentioned in detail above and below), without chemical synthesis steps (although they can also be prepared or modified by chemical synthesis, for example, acylated or similar), or be modified by certain downstream processing procedures (for example, permethylated under the influence of acid methanol) and are thus present as extracts or purified components of the extracts, and not derivatives only obtainable by chemical synthesis.
[0085] [00085] They can also be present and used as part of an extract that is obtainable by extracting a fungus or a part of an appropriate fungus of the genus Dacryopinax.
[0086] [00086] "Substantially" preferably means that the corresponding impurities are present only in minimal amounts, for example, in less than 5% by weight, less than 4% by weight, less than 3% by weight, less than 2% by weight, less than 1% by weight, less than 0.5% by weight or less than 0.2% by weight, based on the complete weight of the corresponding dry extract or compound of formula I or mixture of compounds of formula I.
[0087] [00087] In the context of the present invention, the terms "essentially consists of" or "essentially consisting of" mean that the part of the total weight is 90% by weight or more, preferably 95% by weight or more, more preferably 98% in weight or more, more preferably still 99% by weight or more, in each case based on the total amount used. For example, a "mixture essentially consisting of" means that the total amount of the constituents, as defined in the respective case, is 90% by weight or more, preferably 95% by weight or more, more preferably 98% by weight or more, more preferably still 99% by weight or more, in each case based on the total weight of the mixture.
[0088] [00088] The term "glycolipid" can be replaced by "glycosylated fatty acid" too, where it is used in relation to the compounds of formula I.
[0089] [00089] "A compound of the formula I" or "compound (s) of the formula I" can refer to one or more compounds of the formula I, which is a compound or a mixture of compounds of the formula I, or the USE of a compound of formula I, where the reference to the compound (s) of formula I always includes the compound (s) as such or in the form of a salt (especially a physiologically salt, ie for example, pharmaceutical, nutraceutical or cosmetically acceptable), a solvate and / or a tautomer thereof, or in the form of lactone. In all cases, this means that only one compound (in substantially pure form or as a direct extract or an additionally enriched extract) or a mixture of two or more compounds of formula I (which mixture is preferred) may be present, for example. for example, in a pharmaceutical, nutraceutical or cosmetic extract or formulation according to the invention, or that he or they can be of use according to the invention.
[0090] [00090] The compounds of formula I can also be esterified to their free carboxyl group shown in formula I, on the right side, with alcohols, for example, alcohols with 1 to 10 carbon atoms, such as alkanols, for example, C1-C7 alkanols, such as methanol or ethanol, phenyl-C1-C4 alkanols, such as benzyl alcohol, or the like. Compounds not esterified to the carboxyl group in formula I on the right side are preferred.
[0091] [00091] Preferably, the total weight portion of the compound or all compounds of formula I in an extract or mixture of compounds of formula I or a purified compound of formula I which is of use according to the invention in the final extract , in the mixture or in the compound (direct or additionally enriched) is in the range of 0.01% to 100% by weight, more preferably from 1% to 100% or to 99% by weight, in another modality from 5% to 100% or 99% by weight, or 20% to 100% or 95% by weight, or for example, 50% to 100% or 90% by weight.
[0092] [00092] When used in the invention, the "%", for example, the percentage is defined by the weight portion of the part of interest of the total weight, for example, the% is indicated as% by weight; except where otherwise explicitly defined.
[0093] [00093] For the purpose of the invention, the term "carbohydrate" is used in accordance with IUPAC recommendations (Pure and Applied Chemistry, 1995, 67, 1307). The term "carbohydrate having 3 to 30 (preferably 6 to 18) carbon atoms attached via one of its oxygen atoms" especially refers to the mono, oligo- or polysaccharidyl moieties attached via one of its oxygen atoms . The carbohydrates that form the basis for such portions include, but are not limited to, monosaccharides, disaccharides, additional oligosaccharides, or polysaccharides.
[0094] [00094] Monosaccharide, for example, includes, but is not limited to, aldotrioses, such as glyceraldehyde, ketotrioses, such as dihydroxyacetone, aldotetroses, such as erythrosis and threose, ketotetroses, such as erythrulose, aldopentaes, such as arabinose, lixose, ribose and xylose, and deoxypentoses, such as deoxyribose; ketopentoses, such as ribulose and xylulose; hexoses, especially aldo-hexoses, such as alose, altrose, galactose, glucose, gulose, idose, mannose and talose, or keto-hexoses, such as fructose, psychosis, sorbose and tagatose, or deoxy-hexoses, such as rhamnose, cimarose , fucose, 2-deoxyglucose or 2-deoxygalactose; heptoses, such as mano-heptulose, sedo-heptulose; octoses, such as octolose, 2-keto-3-deoxy-mano-octonate; nonoses, such as sialosealose.
[0095] [00095] Disaccharides, for example, include, but are not limited to, trehalose, sucrose, kojibiose, sophorose, nigrosis, laminaribiosis, maltose, cellobiosis, isomaltose, gentiobiose, lactose, melibiosis, neohsperidosis, rutinosis, primeverose, sambiosis latirose and maneuvering.
[0096] [00096] Oligosaccharides, for example, include, but are not limited to raffinose, nystosis, panose, cellotriosis, maltotriose, maltotetraose, xylobiosis, galactotetraose, isopanosis, cyclodextrin (alpha-CD) or cyclomaltohexose, beta-cyclodextrin -CD) or cyclomaltoheptaose and gamma-cyclodextrin (gamma-CD) or cyclomaltooctaose. Polysaccharides, for example, include, but are not limited to, xylan, mannan, galactan, glycan, arabinan, pustulan, gelan, guaran, xanthan, and hyaluronan. Some examples include, but are not limited to, starch, glycogen, cellulose, inulin, chitin, amylose and amylopectin.
[0097] [00097] In the case of di-, tri- and oligosaccharides, the bonds between the carbohydrate subunits can include several possible types, for example, preferably in the form of glycosidic bonds of types 1➜ 2, 1➜ 3, 1➜ 4 and 1➜ 6, in particular the glycosidic bonds are of the 1➜ 2 type.
[0098] [00098] Carbohydrate portions of trisaccharides, especially the formula
[0099] [00099] Preferably, the non-substituent carbohydrate moieties resulting from acylation or etherification have 15 to 18 carbon atoms and they are specially selected from the hexopyranosyl-pentopyranosyl-pentopyranoside type, such as beta-D-glycopyranosyl- (1 ➜ 2) -beta-D-xylopyranosil- (1➜ 2) -beta-D-xylopyranoside, or of the hexopyranosyl-pentopyranosyl-hexopyranoid type, such as beta-D-glycopyranosil- (1➜ 2) -beta-D-xylopyranosyl - (1 ➜ 2) -beta-D-glycopyranoside.
[0100] [000100] Carbohydrates can carry one, more or all of the hydroxyl groups in modified form, for example, as etherified hydroxyl or especially esterified hydroxyl, as defined below, respectively, for example, in a form acylated by a C2-C10- alkanoic, for example, acetylated form, for example, mono- or di- or tri- or tetra-acetylated. A particularly preferred modified form is represented by those compounds of the formula I that have one or more hydroxyl groups in the carbohydrate portion that are acylated by an isovaleryl (3-methyl-butanoyl) portion - these compounds are new and, therefore, also as such they form an embodiment of the invention.
[0101] [000101] Individual compounds of formula I with an acyl substituent with more than 2 carbon atoms, such as (and preferably) an isovaleryl substituent (and preferably a single isovaleryl substituent), in the carbohydrate moiety typically exhibit stronger antimicrobial activity, particularly against yeasts and fungi, especially against yeasts and bottoms of relevance to the deterioration of food, beverages and / or cosmetics, and / or a broader spectrum of activity than the corresponding compounds with a substituent of acetyl in the R carbohydrate portion.
[0102] [000102] Additional esters can be acetates; propionates; butyrates; isobutyrates; valerates, such as n-pentanoate or 2-methyl butyrate, or unsaturated derivatives, such as, but not limited to 2-methyl 2-butenoate (eg, angeloate or tiglate), 3-methyl 2-butenoate or 3-methyl 3-butenoate (senecioate), or hydroxylated derivatives, such as 2-methyl 3-hydroxy butyrate or 2-hydroxymethyl butyrate; or hexenoates, such as n-hexanoate (caproate), isohexanoates, such as, but not limited to 2-methyl valerate, 3-methyl valerate, 4-methyl valerate, 2,3-dimethyl butyrate, or unsaturated derivatives, for example, 2-ethyl 2-butyrate, 2-methyl 2-pentenoate, 4-methyl 2-pentenoate; or aminoacyl, for example, alanyl, cysteinyl, aspartyl, glutamyl, phenylalanyl, glycyl, histidyl, isoleucyl, lysyl, leucyl, methionyl, asparaginyl, pyrrolysinyl, prolyl, glutaminyl, arginyl, seryl, threonyl, selenocysteine, trilinyl, trilinyl, tyrosine, trilinyl, trilinyl, tyrosine, trilinyl, trilinyl, trilinyl, trilinyl, tyrosine. The acylated forms can preferably be natural products, however they can be products of chemical or enzymatic acylation, for example, using active forms of acids and, where required to prevent the reaction of other functional groups, the introduction and, especially to obtain the product final, the removal of the protective groups ("Pg").
[0103] [000103] The protection of such functional groups by such protecting groups ("Pg"), the protecting groups themselves, and their removal reactions are described, for example, in standard reference works, such as JFW McOmie, "Protective Groups in Organic Chemistry ", Plenum Press, London and New York 1973, in TW Greene and PGM Wuts," Protective Groups in Organic Synthesis ", Third edition, Wiley, New York 1999, in" The Peptides "; Volume 3 (editors: E. Gross and J. Meienhofer), Academic Press, London and New York 1981, in "Methoden der organischen Chemie" (Methods of organic chemistry), Houben Weyl, 4th edition, Volume 15 / I, Georg Thieme Verlag, Stuttgart 1974, in H.-D. Jakubke and H. Jescheit, "Aminosauren, Peptide, Proteine" (Amino acids, peptides, proteins), Verlag Chemie, Weinheim, Deerfield Beach, and Basel 1982, and in Jochen Lehmann, " Chemie der Kohlenhydrate: Monosaccharide und Derivate "(Chemistry of carbohydrates; monosaccharides and derivatives), Georg Thieme Verlag, Stuttgart 1974. Especially preferred protecting groups are hydroxyl protecting groups, such as tert-butyldimethylsilyl, methyl, methoxymethyl, or trityl.
[0104] [000104] Chemical acylation can take place with the corresponding acid as such or preferably in the form of a reactive derivative. Reactive (or active) derivatives used as such include halides, for example, chlorides, or nitrophenyl esters, for example, 2,4-dinitrophenyl esters, or acid anhydrides (symmetrical or, for example, with acetic acid) of the carboxy groups of the acids to be reacted.
[0105] [000105] For in situ training, the usual coupling agents can be applied. Such reagents are known to the person skilled in the art and can be conveniently deduced from many sources, for example, Aldrich ChemFiles - Peptide Synthesis (Aldrich Chemical Co., Inc., Sigma-Aldrich Corporation, Milwaukee, WI, USA) Vol 7 No. 2, 2007 (see http://www.sigmaaldri-ch.com/etc/me-dialib/docs/Aldrich/Brochure/al_chemfile_v7_n2.Par.0001 .File.tmp / al _chemfile_v7_n2.pdf). Among the possible coupling agents for the synthesis of the amide and ester bond, the following can be mentioned:
[0106] [000106] Triazoles, uranium or hexafluorphosphonium derivatives, for example, 1-hydroxy-benzotriazole (HOBt), Carbodiimides, for example, dicyclohexylcarbodiimide, active ester forming agents, for example, 2-mercaptobenzothiazole (2-MBT ), azide forming agents, for example, diphenyl phosphoryl azide, acid anhydrides, such as phosphonic propane anhydride, acid halogenating agents, for example, 1-chloro-N, N, 2-trimethyl-1-propenylamine, or similar, or mixtures of two or more such agents.
[0107] [000107] Although the compounds of formula I are preferably obtained by extraction in the form of extracts from natural sources, or further enriched or purified from such extracts (see below), they can also be obtained by chemical synthesis methods.
[0108] [000108] For example, compounds can be synthesized chemically, for example, by a convergent strategy. The glycoside part and the long-chain, unbranched α-hydroxy carboxylic acid part of the molecules are formed separately, with the hydroxyl groups and the carboxylic acid portions being protected by suitable protecting groups. Subsequently, both building blocks are joined by forming a glycosidic bond, using the methods described in the scientific literature. Finally, removal of the protecting groups will result in the desired compounds.
[0109] [000109] The following reaction schemes provide an example of a possible synthetic route: Outline for the overview (see also the following pages):
[0110] [000110] Synthetic methods for glycosylation reactions - including protection, activation and deprotection strategies - are described, for example, in the following literature:
[0111] [000111] J. McMurry; Organic Chemistry, 5th ed .; Brooks / Cole; 2000, 1031; D. E. Levy, P. Fügedi; The organic chemistry of sugars; Taylor and Francis, 2006, 181-197; S. Bufali, P. Seeberger, Org. React. 2006, 68, 303; G.-J. Boons, K.J. Hale, Organic synthesis with carbohydrates. Blackwell Publishing, 2000; R.R. Schmidt, J. Michel, Angew. Chem. Int. Ed. Engl. 1980, 19, 731-732; X.M. Zhu, R.R. Schmidt, Angew. Chem. Int. Ed. 2009, 48, 1900-1934; R. R. Kale et al, Angew. Chem. Int. Ed. 2008, 47, 1265-1268; N. Miquel, S. Vignando, G. Russo, L. Lay, Synlett 2004, 2, 341 - 343; W. Koenigs, E. Knorr, Chem. Ber. 1901, 34, 957981; Fraser-Reid, B .; Tatsuta, K .; Thiem, J., Hrsg., Glycoscience-Chemistry and Chemical Biology, Springer: Berlin, (2001); Lindhorst, T. K., Essentials of Carbohydrate Chemistry and Biochemistry, Wiley-VCH: Weinheim, (2000); Bochov, A. F .; Zaikov, G. E., Chemistry of the O-Glycosidic Bond: Formation and Cleavage, Pergamon Press: Oxford, (1979); Fraser-Reid, B .; Wu, Z .; Udodong, U. E .; Ottosson, H., J. Org. Chem., (1990) 55, 6068-6070; Lemieux, R. U .; Morgan, A. R., Can. J. Chem., (1965) 43, 2190-2198; Sinay, P., Pure Appl. Chem., (1991) 63, 519-528 Toshima, K .; Tatsuta, K., Chem. Rev., (1993) 93, 1503-1531; Evans, W. L .; Reynolds, D. D .; Talley, E. A., Adv. Carbohydr. Chem., (1951) 6, 27-81.
[0112] [000112] Synthetic methods for dihydroxylation reactions are described, for example, in the following literature:
[0113] [000113] R. Brückner: Reaktionsmechanismen, 2. Aufl, Spektrum Verlag, Heidelberg / Berlin 2003, 750-758; MH Junttila, OEO Hormi, J. Org. Chem., 2004, 69, 4816-4820; MH Junttila, OOE Hormi, J. Org. Chem., 2009, 74, 3038-3047; BM Choudary, NS Chodari, K. Jyothi, ML Kantam, J. Am. Chem. Soc., 2002, 124, 5341-5349; GM Mehltretter, S. Bhor, M. Klawonn, C. DӦbler, U. Sundermeier, M. Eckert, H.-C. Militzer, M. Beller, Synthesis, 2003, 295-301; LC Branco, CAM Afonso, J. Org. Chem., 2004, 69, 4381-4389; Krauch, H .; Kunz, H., Reaktionen der Organischen Chemie, 6. Aufl .; Hüthig: eidelberg, (1997); S. 434-436; Hudlicky, T .; Fan, R .; Luna, H .; Olivo, H .; Price, J., Pure Appl. Chem., (1992) 64, 1109-1113; Jacobsen, EN, Acc. Chem. Res., (2000) 33, 421-431; Kolb, HC; VanNieuwenhze, MS; Sharpless, KB, Chem. Rev., (1994) 94, 2483-2547.
[0114] [000114] The synthetic methods for the reactions that join two formation blocks through the generation of double bond are described, for example, in the following literature:
[0115] [000115] Ivin, K. J .; Mol, J. C., Olefin Metathesis and Metathesis Polymerization, Academic Press: New York, (1997); Grubbs, R. H., Handbook of Metathesis, Wiley-VCH: Weinheim, (2003); Bd.1-3; Fürstner, A .; Langemann, K., Synthesis, (1997), 792-803; Fürstner, A., Angew. Chem., (2000) 112, 3140-3172; Blakemore, P. R., J. Chem. Soc., Perkin Trans. 1, (2002), 2563-2585; Staden, L. F., van; Gravestock, D .; Ager, D. J., Chem. Soc. Rev., (2002) 31, 195-200; Wittig, G., Angew. Chem., (1980) 92, 671-675; Schlosser, M .; Christmann, K., Synthesis, (1969), 38-39; Maryanoff, B. E .; Reitz, A. B., Chem. Rev., (1989) 89, 863-927; Murphy, P. J .; Brennan, J., Chem. Soc. Rev., (1988) 17, 1-30; Boutagy, J .; Thomas, R., Chem. Rev., (1974) 74, 87-99; Clayden, J .; Warren, S., Angew. Chem., (1996) 108, 261-291; Brückner, R., Reaktionsmechanismen, 2. Aufl .; Spektrum: Heidelberg, (2003); Ager, D. J., Synthesis, (1984), 384-398; Mukaiyama, T .; Asami, M., Top. Curr. Chem., (1985) 127, 133-167.
[0116] [000116] Furthermore, the present glycolipid compounds of formula I comprise all stereoisomers, such as those that may exist due to asymmetric carbons on the various substituents, including enantiomeric and diastereoisomeric forms. The individual stereoisomers of the glycolipid derivatives of the present invention can, for example, be substantially free of other isomers, or they can be mixed, for example, as racemates or with all others, with more than one of the others, or with two up to less than all the others, selected stereoisomers, for example, diastereoisomers.
[0117] [000117] Especially, the vicinal dihydroxy group in the fatty acid part is, in an embodiment of the invention, to be understood as configured syn and / or anti.
[0118] [000118] To the extent that the compounds of formula I and the salts thereof can exist in their tautomeric form, all such tautomeric forms are contemplated in this document as part of the modalities of the present invention.
[0119] [000119] As the final carboxyl group in the carboxylic acid chain can also form a lactone with one of the hydroxyl groups present on the rest of a molecule of formula I, the compounds of formula I can also be present in the form of lactone, so pure or mixed with the open chain form.
[0120] [000120] The salts of the compound (s) of the formula I are especially the physiologically acceptable salts, that is, salts that have no disturbing toxic, allergenic and / or mutagenic properties on human or animal cells. Such salts can be selected from those known in the art, for example, using calcium, sodium, magnesium, or ammonium as counterions of the carboxylic group or from the salts mentioned below.
[0121] [000121] Where salt-forming groups (for example, acid groups, such as carboxylic acid groups, or basic groups, such as amino or imino groups) are present within them, the glycolipid compounds of formula I may be in the form free or in the form of salts. The term "salt (s)", as used herein, means acid and / or basic salts formed with inorganic and / or organic acids and bases. In addition, when a compound of formula I contains both a basic portion and an acidic portion, "internal salts" can be formed and are included in the term "salt (s)" as used herein. Pharmaceutical or nutraceutical or cosmetically acceptable (i.e., physiologically acceptable, non-toxic) salts are preferred, although other salts are also useful, for example, in the isolation or purification steps that can be employed during preparation. The salts of the compounds of formula I can be formed, for example, by reacting a compound of formula I with an amount of acid or base, such as an equivalent amount, in a medium, such as one in which the salt precipitates or in an aqueous medium, followed by lyophilization or followed by the addition of a water-miscible organic solvent. Ion exchangers can also be used to form salts from free forms or free forms from salts of a compound of formula I. The "free form" refers to the "form without salt-forming counterions", for example, in the form other than salt.
[0122] [000122] Where two active groups with different charge are present, internal or zwitterionic salts can also be formed.
[0123] [000123] Where the compounds of formula I (or glycolipids of formula I or similar) are mentioned in the present description, this also comprises the corresponding salts thereof (in particular, physiologically acceptable), also where not explicitly established, as well as the esters, as well as lactones, or mixtures of two or more of these forms.
[0124] [000124] The compounds of the formula I, which contain an acidic portion (for example, carboxyl groups (-COOH)) can form salts with a variety of organic and inorganic bases. Illustrative basic salts include ammonium salts, non-toxic metal salts derived from metals of groups Ia, Ib, IIa and IIb of the Periodic Table of Elements, for example, alkali metal salts, such as sodium salts, lithium, or potassium, alkaline earth metal salts, such as calcium or magnesium salts, or salts with other metals, such as zinc, salts with organic bases (eg, organic amines), such as mono- , di- or tri-alkylamines unsubstituted or substituted with hydroxy, especially mono-, di- or tri-lower alkylamines, or quaternary ammonium compounds, for example, with alkyl amines, for example, t-butyl amine, N -methyl-N-ethylamine, diethylamine, triethylamine, mono-, bis- or tris- (2-hydroxy-lower alkyl) amines, such as mono-, bis- or tris- (2-hydroxyethyl) amine, 2-hydroxy- tert-butylamine or tris (hydroxymethyl) methylamine, N, N-di-lower alkyl-N- (hydroxy-lower alkyl) -amines, such as N, N-dimethyl-N- (2- hydroxyethyl) -amine or tri- (2-hydroxyethyl) -amine, or N-methyl-D-glucamine; or the cyclic amines, such as piperidine, N-lower alkyl piperidine, for example, N-methyl-piperidine, piperazine, or the quaternary ammonium salts formed by common processes among the amines defined above, such as the tetrabutylammonium salts , or with benzathines, dicyclohexylamines, N-methyl-D-glucamines, N-methyl-D-glucamides, purines, caffeine, theobromine, hydrabamine, choline, betaine, or salts with amino acids, such as arginine, lysine, histidine It's similar. Also salts with the pharmaceutical and / or nutraceutical carrier materials forming salts are possible and included by the invention. ISOLATION
[0125] [000125] To obtain the compounds of formula I, as such or comprised in an extract, preferably the desired substances of formula I are isolated from natural sources, with subsequent chemical modification (for example, acylation) or preferably without such chemical modification.
[0126] [000126] The purpose of the extraction stage and especially the isolation stage is to retain the desired substances. The desired substances in the present context are any substances that directly or indirectly contribute to the preservative properties of the composition, provided that one or more compounds of formula I are also included. Isolation can be carried out by isolating or separating the one or more compounds of formula I according to chemical and / or physical properties. Examples of chemical properties include affinity for one or more compounds and chemical stability. Examples of physical properties include mass or size, charge, solubility, polarity, distribution, surface absorption, melting point, and the like.
[0127] [000127] The natural compounds of formula I, or extracts comprising one or more of them, for USE in, or in accordance with, the present invention are isolated from one or more cultures, especially liquid cultures, of mushrooms of the genera listed above or below, for example, with the genetic characteristics provided in detail below.
[0128] [000128] By the term "extract", we mean a direct extract (in liquid form, preferably dried), for example, obtained as described below, or preferably an additionally enriched extract (obtainable, for example, by one or more steps additional purification steps, after extraction, for example, chromatography, for example, as described below) containing one or more, preferably two or more compounds of formula I.
[0129] [000129] The compound (s) of the formula I in the form of an extract and the extracts according to the invention can be obtained especially and preferably by extracting liquid cultures, especially cultures of liquid or solid mycelium, of mushrooms of the genus Dacryopinax, for example, mushrooms or their parts of the species Dacryopinax, of mushrooms of the genus Ditiola, for example, mushrooms or their parts, and / or mushrooms of the genus Femsjonia luteo-alba, for example, mushrooms or its parts, especially the species and more especially the strains deposited as defined above.
[0130] [000130] Extracts according to the invention or useful according to the invention can be produced according to any suitable process, preferably comprising the extraction of one or more compounds of formula I. The term "extract", whenever used, it also includes precipitates, for example, produced as described below.
[0131] [000131] For example, the extraction of one or more compounds and / or the mixture of compounds of formula I from a cultivated mushroom (especially from the submerged mycelium culture) or part of the mushroom of the genera mentioned above by means of a solvent lipophilic (preferably non-aqueous).
[0132] [000132] Extraction, therefore, can take place with a non-polar or weakly polar solvent (meaning less polar than water) or a mixture of solvents, meaning that the preferred obtainable or obtained extracts according to the invention are lipophilic extracts.
[0133] [000133] Preferably, the polarity is defined by an Et (30) value of 56 kcal / mol or less (at 25 ° C and 100 kPa (1 bar)), for example, 52 kcal / mol or less (at water has an Et (30) of 63.1). The method of Et (30) is based on a method published by Reichart et al. and makes use of the stabilization of the fundamental state of the betaine dye 2,6-diphenyl-4- (2,4,6-triphenyl-1-pyridinium) phenolate, CAS No 10081-39-7, in nonpolar solvents, resulting in an energy greater for the transition from the ground state (HOMO) to the first excited state (LUMO) of the molecule (see K. Dimroth, J Lieb Ann d Chemie (1963) 661 (1): 1 - 37, DOI 10.1002 / jlac.19636610102) .
[0134] [000134] Examples of suitable solvents are organic solvents (two or more of which can also be mixed), for example, a ketone or an ester, such as acetone and / or ethyl acetate, an ether, for example , a cyclic ether, such as dioxane, and / or (also in a specific embodiment) an alcohol, for example, ethanol, and / or a liquid or superfluid gas, especially superfluid CO2.
[0135] [000135] Alternatively, the extract can be obtained by bringing a culture supernatant to a slightly alkaline pH, for example, by adding an alkali metal hydroxide, such as sodium or potassium hydroxide, separating any solid material, for example, mycelia or other solid components, eg by microfiltration, acidifying the filtrate by adding an acid, for example, an organic or inorganic acid, such as hydrohalide, such as hydrochloride, to an appropriate pH, for example example, less than pKa (which can, for compounds of formula I, be assumed to be in the range of about 4.0 to 5.0, for example, 4.2 to 4.5), removing the supernatant from a precipitate obtained (which comprises the compounds of formula I) and optionally washing the precipitate and / or extracting the compounds of formula I at an appropriate pH in a less polar solvent, for example, one as mentioned above. This process (also resulting in what is called an "extract" in the present description) is especially preferred since it results in high yield and helps to avoid the use of solvents, thus being economically and ecologically advantageous.
[0136] [000136] The addition according to the use or method of the invention preferably occurs by mixing the resulting extract or the isolated compound (s) of formula I in such material or by impregnating or coating it with the ( s) compound (s) of the formula I as such or in an appropriate composition (for example, liquid).
[0137] [000137] For preservative or antimicrobial compositions, additional processing steps may precede and / or follow, such as drying (for example, lyophilization, spray drying, fluid bed or spouted bed or evaporation), granulation, agglomeration, concentration (for example, even syrups, formed by means of concentration and / or with the aid of thickeners), pasteurization, sterilization, freezing, dissolving, dispersing, filtering, centrifuging, cooking, and the like .
[0138] [000138] The compounds of formula I have been surprisingly found to show especially preservative or antimicrobial purposes (the term "antimicrobials" especially referring to treated materials that are not treated to prevent deterioration of themselves, but are to be used in an uncontaminated form by microbes, for example, implants or similar, although the term "preservative activity" also includes antimicrobial activity, but also another stabilizing activity, for example, by emulsification or acidification due to the addition of the compound (s) of formula I perishable products).
[0139] [000139] The preservative and antimicrobial properties can conveniently be shown by methods known in the art, for example, as described below and in the Examples.
[0140] [000140] In such tests, antimicrobial and preservative activity can be shown.
[0141] [000141] The term "increase stability against microorganisms" refers to inhibiting growth or killing microorganisms, thereby providing a material provided according to the invention with one or more compounds of formula I with protection against damage, films or microbial degradation.
[0142] [000142] Among the materials to which one or more compounds of formula I can be added, the following can be mentioned: A material selected from the group consisting of a cosmetic, a home care product, a food, a drink, a pharmaceutical substance, a medical device, and an active packaging material. Also included are semi-finished products or precursors, for example, especially in the case of beverages or foods, ready-to-use powders or concentrates. Food and drinks
[0143] [000143] The term "food", sometimes also called "foodstuff", means articles used for food (or drink) for man or other animals, chewing gum, and articles used for the components of any such article. The term "food" especially refers to materials, usually of plant, animal or other organism origin, which comprise the body's nutrients, such as carbohydrates, fats, proteins, vitamins and / or minerals, and are ingested and assimilated by the body human or animal to produce energy, stimulate growth and sustain life. Normally, the food has a preferably solid form, but it can also be almost liquid, for example, in the case of yogurt or similar. The "food" includes a raw, cooked, or processed edible substance, the cream, or the ingredient used or intended for use or for sale whole or in parts for human or animal consumption, or chewing gum.
[0144] [000144] "Drink" means a liquid product to drink, usually including water, which can be consumed to quench thirst, provide nutrition, for the purposes of satisfaction or pleasure and / or for other functional purposes (for example, to administer medicines or other functional materials). Among liquids for human or animal consumption, those that qualify as juice, beverage (including soda, such as lemonade), non-alcoholic or alcoholic beverage, and / or cocktail can be mentioned.
[0145] [000145] "Juice" means the aqueous liquid squeezed or extracted from one or more fruits or vegetables, the purees of the edible parts of one or more fruits or vegetables, or any concentrates of such liquid or puree.
[0146] [000146] Use as an agent with preservative properties of a compound or compounds of formula I also includes use in precursor beverage products, for example, concentrates, syrups and / or powders will be reconstituted in a drink towards the invention by adding water.
[0147] [000147] The term "baby food" means a food that is intended to be or is represented for special dietary use only as a food for children, because of its simulation of human milk or its suitability as a complete or partial substitute for milk human.
[0148] [000148] Drinks can be alcoholic and / or non-alcoholic, carbonated and / or non-carbonated.
[0149] [000149] Drinks include milk substitutes, and the like.
[0150] [000150] Drinks may include water, flavored water, fortified water, flavored drinks, sparkling water, for example, sparkling mineral water or sodas, juices, Coca-cola, lime soda and lemon (“lemon lime”), ginger soda (“ginger ale”), and root beer soft drinks (“root beer”), which are carbonated in the style of soft drinks, as well as drinks that provide health benefits. health or well-being from the presence of metabolically active substances, such as vitamins, amino acids, proteins, carbohydrates, lipids, or their polymers, where such products can also be formulated to contain milk, coffee, or tea (for example, green tea) or other botanical solids, syrup, diet drinks, carbonated soft drinks, fruit juices, for example, orange juice, grapefruit juice, apple juice, red grape juice, white grape juice , pear juice, concord grape juice, pineapple juice, pomegranate juice, s cranberry juice, passion fruit juice, lime juice, lemon juice, mango juice, guava juice, banana juice, red and black currant juice, cashew juice, cantaloupe juice, apricot juice , blackberry juice, alpine blackberry juice, blackberry juice, gooseberry juice, sour wild apple juice, prune juice, plum juice, kiwi juice, strawberry juice, grape juice- blueberry juice, red raspberry juice, blackberry juice, cherry juice, watermelon juice, peach juice, nectarine juice, logan berry juice, honeydew melon juice, papaya juice, boysenberry, youngberry juice, rhubarb juice, soursop juice, açaí juice, goji juice, fig juice, cob juice, date juice, carambola juice, acerola juice, quince juice, grape juice- mountain juice, tangerine juice, drinks containing fruit, for example, fruit drinks that provide the flavor of any of the, for example, fruit juices mentioned above and with n have more than 0% fruit juice, but less than 100% fruit juice, fruit flavored drinks, vegetable juices, eg tomato juice, beet juice, carrot juice, celery juice , drinks containing vegetables, which provide the flavor of any of the aforementioned vegetable juices and contain more than 0% vegetable juice, but less than 100% vegetable juice, isotonic drinks, non-isotonic drinks, soft drinks containing a fruit juice, coffee, tea, tea drinks prepared from concentrate, extracts, or tea powders, drinkable dairy products, for example, drinkable yoghurts (drinking yoghurt), sour milk or buttermilk, hot chocolate, powders / mixtures for chocolate, drinkable soy products, milk substitutes, for example, coconut milk, alcoholic beverages, for example, malt beverages, wine, beer, distilled alcoholic beverages, distilled beverages, sparkling wine, champagne or liquors, milk fruit shakes, orc hata (vegetable and / or rice components made into a drink), sports drinks, energy drinks, health drinks, wholesome drinks, protein drinks (e.g. dairy, soy, rice or other), drinkable soy yogurts, low acid drinks as defined in US 21 CFR Part 113. Acidified drinks as defined in US21 C.F.R. Part 114, nectars, tonics, frozen fizzy drinks, frozen non-carbonated drinks, liquid meal replacements, infant formulations, and combinations or mixtures thereof.
[0151] [000151] It is also possible to formulate such drinks to contain one or more nutraceuticals. In this document, a nutraceutical is a substance that has been shown to possess at least a specific health benefit or a sense of well-being, as documented in professional journals or texts. Nutraceuticals, however, do not necessarily act to cure or prevent certain specific types of medical conditions.
[0152] [000152] Without considering one or more compounds of formula I, food or drink may comprise additional usual additives for food and / or drink.
[0153] [000153] For the purpose of the invention, "additives" in the sense of "sweeteners" are substances used to impart a sweet taste to foods (this term in this paragraph also including drinks) or in table top sweeteners; "antioxidants" are substances that prevent the oxidation of components, for example, preventing the material from becoming rancid; "dyes" are substances that add or restore color in a food, and include the natural constituents of food and natural sources that are not normally consumed as food as such and are not normally used as characteristic ingredients of the food. The preparations obtained from food and other materials from edible natural sources, obtained by physical and / or chemical extraction resulting in a selective extraction of the pigments in relation to the nutritional or aromatic constituents, are dyes within the meaning of this Regulation; "preservatives" are substances that prolong the shelf life of foods by protecting them against deterioration caused by microorganisms and / or that protect against the growth of pathogenic microorganisms; "antioxidants" are substances that prolong the shelf life of foods by protecting them against deterioration caused by oxidation, such as rancidity of fat and changes in color; "vehicles" are substances used to dissolve, dilute, disperse or otherwise physically modify a food additive or flavoring, food enzyme, nutrient and / or other substance, added for nutritional or physiological purposes to a food, without changing its function (and without exercising any technological effect themselves), to facilitate its handling, application or use; "acids" are substances that increase the acidity of a foodstuff and / or give it an acidic flavor; "acidity regulators" are substances that alter or control the acidity or alkalinity of a foodstuff; "pie anti-forming agents" are substances that reduce the tendency of individual particles in a foodstuff to adhere to one another; "defoaming agents" are substances that prevent or reduce foaming; "augmentation agents" are substances that contribute to the volume of a foodstuff, without significantly contributing to its available energy value; "emulsifiers" are substances that make it possible to form or maintain a homogeneous mixture of two or more immiscible phases, such as oil and water, in a foodstuff; "emulsifying salts" are substances that convert the proteins contained in cheese into a dispersed form and, thus, promote the homogeneous distribution of fat and other components; "firming agents" are substances that make or keep tissues of fruit or vegetables firm or lush, or interact with gelling agents to produce or strengthen a gel; "flavor enhancers" are substances that increase the existing taste and / or odor of a foodstuff; "foaming agents" are substances that make it possible to form a homogeneous dispersion of a gas phase in a liquid or solid foodstuff; "gelling agents" are substances that give a texture to the foodstuff through the formation of a gel; "polishing agents" (including lubricants) are substances that, when applied to the outer surface of a foodstuff, give a glossy appearance or provide a protective coating; "humectants" are substances that prevent food from drying out by neutralizing the effect of an atmosphere having a low degree of humidity, or promoting the dissolution of a powder in an aqueous medium; "modified starches" are substances obtained by one or more chemical treatments of edible starches, which may have undergone a physical or enzymatic treatment, and may be diluted or bleached with acid or alkali; "packing gases" are gases other than air, introduced into a container before, during or after placing a foodstuff in this container; "propellants" are gases other than air that expel a foodstuff from a container; "bulking agents" are substances or combinations of substances that release gas and thereby increase the volume of a dough or a dough with eggs and milk; "kidnappers" are substances that form chemical complexes with metal ions; "stabilizers" are substances that make it possible to maintain the physical-chemical state of a foodstuff; stabilizers include substances that enable the maintenance of a homogeneous dispersion of two or more substances immiscible in a foodstuff, substances that stabilize, retain or intensify an existing color of a foodstuff and substances that increase the binding capacity of the foodstuff , including the formation of cross-links between proteins, enabling the binding of pieces of food to the reconstituted food; "thickeners" are substances that increase the viscosity of a foodstuff; "fine flour treatment agents" are substances, other than emulsifiers, which are added to fine flour or dough to improve its cooking quality.
[0154] [000154] Among the known preservatives for food and beverages (also called additional (chemical) preservatives in this document), the following can be mentioned, without excluding others: benzoic acid, sodium benzoic acid salt, potassic benzoic acid salt, salt calcium benzoic acid, propionic acid, salicylic acid, sorbic acid, sodium salt of sorbic acid, potassium salt of sorbic acid, calcium salt of sorbic acid, ethyl parahydroxybenzoate, ethyl parahydroxybenzoate, propyl parahydroxybenzoate , sodium parahydroxybenzoate propyl, methyl parahydroxybenzoate, sodium parahydroxybenzoate, sulfur dioxide, sodium sulfite, sodium hydrogen sulfite, sodium metabisulfite, potassium metabisulfite, calcium sulfite, calcium sulfite hydrogen , biphenyl or diphenyl, orthophenyl phenol, orthophenyl sodium phenol, thiabendazole, nisin, natamycin or pimaracin, formic acid, sodium formate, calcium formate, hexamethylene tetr amine or hexamine, formaldehyde, dimethyl dicarbonate, sodium nitrite, potassium nitrite, sodium nitrate, potassium nitrate, acetic acid, sodium acetates, for example, sodium hydrogen acetate, potassium acetate, calcium acetate, acetate ammonium, lactic acid, propionic acid, sodium propinate, potassium propionate, calcium propionate, uric acid, sodium tetraborate (borax), invertase, lysozyme.
[0155] [000155] Preferably, however, no such preservatives are added in the embodiments of the present invention.
[0156] [000156] The consumer articles of the present invention, for example, beverages, can have a pH ranging from 1.5 to 10, for example, from about 1.5 to about 4.6. It is known in the art that the pH of a drink can be a factor in keeping a drink stable on the shelf, since the growth of some microorganisms can be prevented under acidic conditions. This, however, is not the case for acidophilic microorganisms, such as Lactobacillus, Saccharomyces and Candida, which thrive in such an acidic environment. The use of the present invention allows the composition to maintain microbial stability even in view of these acidophilic microorganisms.
[0157] [000157] For an acidic drink (pH <4.6), the acidity of the drink can be adjusted to, and kept within, the range referred to by methods known and conventional in the art. For example, the pH can be adjusted using one or more acidulants, also called acidity regulator (s), for example, as defined below.
[0158] [000158] In addition, the use of acidity regulators can assist in microbial inhibition, while maintaining the pH of the drink. The compositions of the present invention, however, can inherently have a desirable pH, without the use of any acidity regulator or other components to modify the pH. Thus, the incorporation of at least one acidity regulator is optional in the compositions of the present invention.
[0159] [000159] Furthermore, the amounts of the acidity regulator (s), which may be present in the composition according to the present description, are those conventionally used in beverage compositions. For example, at least one acidulant can be present in an amount ranging from about 0.01% to about 1% by weight with respect to the composition.
[0160] [000160] One aspect of the invention is aimed at conserving a wide range of beverage products that have a pH of less than 7.5, in particular less than about 4.6, such as 2.5 to 4.6 , against deterioration by yeast, fungus and a variety of acid-tolerant bacteria. The preservation of the product can be carried out merely by adding the chemical agents described in this document, however it is also possible to supplement the action of chemical substances with purely physical forms of conservation, such as changing the temperature of the product, different lengths of irradiation waves , pressure or their combinations. In certain illustrative embodiments, the pH of the beverage product comprising the preservative system is, for example, about 4.6 or less, about 2.5 to about 4.4, about 2.6 to about 4 , 5.
[0161] [000161] The acidity regulator (s) may be in a non-dissociated form or in their respective salt form, such as the potassium, sodium, or hydrochloride salts, or be a mixture, thereby forming a type of buffer to a desired pH. Among acidity regulators (pH regulators), mention may be made of organic and inorganic acids to be used in adjusting the pH of a composition of the present invention, such as a drink, for example, acetic acid, sodium acetates, for example, sodium hydrogen acetate, potassium acetate, calcium acetate, ascorbic acid, sodium ascorbate, potassium ascorbate, carbon dioxide, sodium carbonates, including sodium hydrogen carbonate ( sodium bicarbonate) and sodium sesquicarbonate, potassium carbonates, for example, potassium hydrogen carbonate, ammonium carbonates, for example, ammonium hydrogen carbonate, magnesium carbonates, for example, carbonate hydroxide magnesium (sin., magnesium hydrogen carbonate), malic acid, fumaric acid, sodium fumarate, potassium fumarate, calcium fumarate, calcium citrates, including mono, di or tricalcium salts, triamonium citrate, the ammonium ferrocitrate, sodium malates, for example, sodium hydrogen malate, potassium malate, calcium malates, for example, calcium calcium malate, adipic acid, sodium adipate, potassium adipate , succinic acid, 1,4-heptonolactone, potassium chloride, calcium chloride, ammonium chloride or ammonia solution, magnesium chloride, stannous chloride, sodium sulfates, for example, sodium hydrogen sulphate, potassium sulphates, for example, potassium hydrogen sulphate, calcium sulphate, ammonium sulphate, magnesium sulphate or Epsom salts, copper sulphate, aluminum sulphate, sodium aluminum sulphate, aluminum potassium sulphate, aluminum ammonium sulphate, sodium hydroxide, potassium hydroxide, calcium hydroxide, ammonium hydroxide, magnesium hydroxide, calcium oxide, calcium oxide magnesium, sodium ferrocyanide, potassium ferrocyanide, calcium ferrocyanide, dicalcium diphosphate, tartaric acid, sodium tartaric acid, potassium tartaric acid, glyconic acid, glucono-delta-lactone, or mixtures of two or more of them. Note that the compounds of formula I, due to their acid / base properties, can also be used to regulate the pH of a composition that comprises them.
[0162] [000162] Among the emulsifiers, the following can be mentioned:
[0163] [000163] lecithins; metatartaric acid, calcium tartrate; alginic acid and the sodium, potassium, ammonium and calcium salts; propane-1,2-diol alginate; the agar; Carrageenan; processed eucheuma kelp; locust bean gum; guar gum; tragacanth; acacia gum; gum arabic; xanthan gum; the Goma caraia; the Goma of the fake brazilwood; gellan gum; glycerol; Konjac, konjac gum, glycomanan konjac; soy emicellulose; Cassia Gum; polyoxyethylene stearate (8); polyoxyethylene sorbitan monolaurate, polysorbate 20; polyoxyethylene sorbitan monooleate, polysorbate 80; polyoxyethylene sorbitan monopalmitate, polysorbate 40; polyoxyethylene sorbitan monostearate, polysorbate 60; polyoxyethylene sorbitan tristearate, polysorbate 65; pectins and amidated pectin; ammonium phosphatides; sucrose isobutyrate acetate; glycerol esters from wood resins; diphosphates and salts, disodium diphosphate, trisodium, tetrasodium diphosphate, dipotassium diphosphate, tetrapotassium diphosphate, dicalcium diphosphate, calcium dihydrogen diphosphate; triphosphates and salts, pentasodium, pentapotassium; polyphosphates and salts, sodium, potassium, sodium calcium, calcium, sodium aluminum, aluminum; beta-cyclodextrin; cellulose, in powder or microcrystalline and derivatives, methyl-, ethyl-, hydroxypropyl-, hydroxypropyl methyl-, ethyl methyl-, carboxymethyl-, carboxymethyl- sodium cross-linked, enzymatically hydrolyzed methyloxy; the sodium, potassium and calcium salts of fatty acids; magnesium salts of fatty acids; mono- and diglycerides of fatty acids; acetic acid esters of mono- and diglycerides of fatty acids; the lactic acid esters of mono- and diglycerides of fatty acids; the citric acid esters of mono- and diglycerides of fatty acids; the tartaric acid esters of mono- and diglycerides of fatty acids; esters of mono- and diacetyltartaric acid of mono- and diglycerides of fatty acids; esters of mixed acetic and tartaric acids of mono- and diglycerides of fatty acids; the sucrose esters of fatty acids; saccharoglycerides; polyglycerol esters of fatty acids; polyglycerol polyrricinoleate; the propane-1,2-diol esters of fatty acids; thermally oxidized soybean oil interacted with mono- and diglycerides of fatty acids; sodium stearoyl-2-lactylate; calcium stearoyl-2-lactylate; stearyl tartrate; sorbitan monostearate; sorbitan tristearate; sorbitan monolaurate; sorbitan monooleate; sorbitan monopalmitate; invertase; silicon dioxide (Silica); magnesium stearate, calcium stearate; oxidized starch; acetylated diamide phosphate; octenyl sodium succinate starch; acetylated oxidized starch, or mixtures of two or more of them.
[0164] [000164] As antioxidants, among others, the following can be mentioned:
[0165] [000165] ascorbic acid and salts, sodium, calcium; ascorbic acid fatty acid esters; tocopherols, alpha-tocopherol, gamma-tocopherol, delta-tocopherol; propyl gallate; octyl gallate; dodecyl gallate; erythorbic acid, sodium erythorbate; tertiary butyl hydroquinone (TBHQ); butylated hydroxyanisole (BHA); butylated hydroxytoluene (BHT); rosemary extracts; 4-hexylresorcinol, or mixtures of two or more of them.
[0166] [000166] Such additives may be present in relative quantities, considering the complete composition of the food or drink product concerned, in quantities totaling from 0.01% to 90% by weight, for example, from 0.05% to 50% in weight, for example, from 0.1% to 5% by weight or from 0.2% to 20% by weight. Cosmetics
[0167] [000167] The compounds of formula I, in view of their preservative properties, are also useful in supporting or providing conservation of cosmetics.
[0168] (1) um artigo, por exemplo, uma mistura ou substância ou produto, pretendido ser colocado em contato com as diversas partes externas do corpo humano ou corpo animal (epiderme, sistema de cabelos, unhas, lábios e órgãos genitais externos), por exemplo, que possa ser esfregado, derramado, espalhado, ou pulverizado sobre, ou de outro modo aplicado ao,corpo humano ou qualquer parte dele, incluindo a cavidade oral e os dentes, a pele e o cabelo, com a finalidade exclusiva ou principalmente de limpá-las, perfumá-las, alterar o seu aspecto e/ou corrigir os odores do corpo, e/ou protegê-las ou mantê-las em boa condição; e (2) artigos pretendidos para uso como um componente de quaisquer tais artigos; explicitamente incluindo o sabão. [000168] The term "cosmetic" is intended here to mean (1) an article, for example, a mixture or substance or product, intended to be placed in contact with the various external parts of the human body or animal body (epidermis, hair system, nails, lips and external genitals), for example , which can be rubbed, poured, spread, or sprayed on, or otherwise applied to, the human body or any part of it, including the oral cavity and teeth, skin and hair, for the sole purpose or primarily of cleaning them, perfume them, change their appearance and / or correct body odors, and / or protect or keep them in good condition; and (2) articles intended for use as a component of any such articles; explicitly including soap.
[0169] [000169] The term cosmetics, as used here, also includes "personal care products" and "personal hygiene products", such as menstrual care products, handkerchief fabrics and the like.
[0170] [000170] For example, cosmetic products include, but are not limited to:
[0171] [000171] Creams, emulsions, lotions, gels and oils for the skin (hands, face, feet, etc.); facial masks (with the exception of peeling products); colored bases (liquids, pastes, powders); makeup powders, powders after bath, hygienic powders, etc .; soaps, deodorant soaps, etc .; perfumes, toilet waters and cologne; bath and shower preparations (salts, foams, oils, gels, etc.); depilatories; deodorants and antiperspirants; hair care products (hair dyes and bleaches; products for curling, straightening and fixing); products to define; cleaning products (lotions, powders, shampoos, hand washing products, disinfectant products (for hands); conditioning products (lotions, creams, oils); hairdressing products (lotions, lacquers, glosses); shaving products ( creams, foams, lotions, etc.); products for making up and removing make-up from the face and eyes; products intended for application on the lips; products for the care of teeth and mouth (for example, gargle fluids, antiseptic liquid mouthwash or toothpaste); products for nail care and painting; products for external intimate hygiene; products for sunbathing; products for sunless tanning; products for whitening the skin; anti-wrinkle products, absorbent tampons, sanitary napkins , wet cleaners, diapers or wipes.
[0172] [000172] Depending on the field of application, certain cosmetic products mentioned above can also be used in the medical field, in particular certain washing products are suitable as disinfectant products, such as hand disinfectants or instrument disinfectants.
[0173] [000173] In all cases, the antimicrobial properties of the compounds of formula I may provide, as an additional benefit towards an extra premium effect, their antimicrobial efficiency for the cosmetic properties of the formulations, although the purely cosmetic use of the corresponding cosmetics preferably be predominant.
[0174] [000174] Cosmetics can comprise, in relation to their intended use, several active and inactive ingredients, called "cosmetic additives" in the following.
[0175] [000175] Among the cosmetic additives, the following, without limiting the scope of possible additives, can be mentioned:
[0176] [000176] "Abrasives" are substances that remove materials from different surfaces of the body or assist in the mechanical cleaning of teeth or improve gloss, "absorbents" are substances that absorb dissolved or finely dispersed substances, soluble in water and / or oil, "pie anti-formation" are substances that allow free flow of solid particles and, thus, prevent the agglomeration of powdered cosmetics in aggregates or hard masses; "anticorrosives" are substances that prevent packaging corrosion, "anti-dandruff" are substances that help control dandruff, "antifoams" are substances that suppress foam during manufacture or reduce the tendency of finished products to generate foam, "antimicrobials" are substances that help control the growth of microorganisms on the skin, "antioxidants" are substances that inhibit reactions promoted by oxygen, thereby preventing oxidation and rancidity, "antiperspirants" are substances that reduce perspiration, "antiplatelets" are substances that help protect against platelets, "antiseboreal agents" are substances that help control sebum production, "antistatic agents" are substances that reduce static electricity by neutralizing the electrical charge on a surface, "astringents" are substances that contract the skin, "binders" are substances that provide cohesion in the skin. smetics, "whitening" are substances that lighten the tone of skin or hair, "buffering agents" are substances that stabilize the pH of cosmetics, "volume agents" are substances that reduce the mass density of cosmetics, "chelators" are substances that react and form complexes with metal ions that could affect the stability and / or appearance of cosmetics, "cleansers" are substances that help to keep the body surface clean, "cosmetic dyes" are substances that color cosmetics and / or add color to the skin and / or its members (for example, hair or nails) (for example, dyes or pigments, for example, lactoflavin, caramel capsanthin, capsorrubin, beet red, anthocyanins, bromothymol blue, bromocresol green, acid red, aluminum, magnesium, calcium and zinc stearates); "denaturants" are substances that make cosmetics unpleasant to the taste, mainly added to cosmetics containing ethyl alcohol; "deodorants" are substances that reduce or mask unpleasant body odors, "depilatories" are substances that remove unwanted hair from the body; "detanglers" are substances that reduce or eliminate hair entanglement due to changes in the surface of the hair or damage and, thus, help to comb; "emollients" are substances that soften and smooth the skin; "emulsifiers" are substances that promote the formation of intimate mixtures of non-miscible liquids by altering the interfacial tension; "emulsion stabilizers" are substances that help the emulsification process and improve the stability and the life of the emulsion; "film-makers" are substances that produce, on application, a continuous film on the skin, hair or nails; "sparkling" captures several small bubbles of air or other gas within a small volume of liquid by changing the surface tension of the liquid; "foam reinforcers" are substances that improve the quality of the foam produced by a system by increasing one or more of the following properties: volume, texture and / or stability; "gel formers" are substances that give the consistency of a gel (a semi-solid preparation with some elasticity) to a liquid preparation; "hair conditioners" are substances that make hair easy to comb, flexible, soft and shiny and / or add volume, lightness, shine, etc .; "hair dyes" are substances that color hair; "hair fixers" are substances that allow physical control of the hairstyle; "hair curlers or straighteners" are substances that modify the chemical structure of hair, allowing it to be defined in the required way; "humectants" are substances that retain and conserve moisture; "hydrotropic agents" are substances that increase the solubility of the substance which is only slightly soluble in water; "keratolytics" are substances that help eliminate dead cells in the stratum corneum; "masking agents" are substances that reduce or inhibit the basic odor or taste of the product; "moisturizing" compounds increase the water content of the skin and help to keep it soft and smooth; "nail conditioners" are substances that improve the cosmetic characteristics of the nail; "opacifying agents" are substances that reduce the transparency or translucency of cosmetics; "oral care" provides cosmetic effects to the oral cavity, for example, cleaning, deodorizing, protection; "oxidizers" are substances that alter the chemical nature of another substance by adding oxygen or removing hydrogen; "pearlizing agents" are substances that give a pearly appearance to cosmetics; "plasticizers" are substances that soften and make flexible another substance that, otherwise, could not be easily deformed, spread or formulated; "preservatives" (additional preservatives) are substances that mainly inhibit the development of microorganisms in cosmetics; "propellants" are substances that generate pressure in an aerosol canister, expelling the contents when the valve is opened, some liquefied propellants can act as solvents; "reducers" are substances that alter the chemical nature of another substance by adding hydrogen or removing oxygen; "renutridores" are substances that replace the lipids of the hair or of the top layers of the skin; "refreshing" are substances that give a pleasant freshness to the skin; "skin conditioners" are substances that keep the skin in good condition; "skin protectors" are substances that help to avoid the harmful effects to the skin of external factors; "softeners" are substances that seek to achieve a uniform surface of the skin by decreasing roughness or irregularities; "solvents" are substances that dissolve other substances; "mitigators" are substances that help to mitigate the discomfort of the skin or scalp; "stabilizers" are substances that improve the stability and shelf life of the ingredients or formulation; "surfactants" are substances that reduce the surface tension of cosmetics, as well as help the uniform distribution of the product, when used; "tanning" is a process that darkens the skin with or without exposure to UV; "tonics" are substances that produce a feeling of well-being on the skin and hair; "UV absorbers" are substances that protect the cosmetic product from the effects of UV light; "UV filters" are substances that filter certain UV rays to protect the skin or hair from the harmful effects of these rays; "viscosity controllers" are substances that increase or decrease the viscosity of cosmetics.
[0177] [000177] In one embodiment, the formulations may be or comprise or contain cosmetic additives, such as those conventionally used in cosmetic preparations, for example, sunscreens, preservatives, bactericides, fungicides, virucides, refrigerants, insect repellents (for example , DEET, IR 3225, Dragorepel), plant extracts, anti-inflammatory substances, wound healing accelerators (for example, chitin or chitosan and its derivatives), film-forming substances (for example, polyvinylpyrrolidones or chitosan or their derivatives) , usual antioxidants, vitamins (for example, vitamin C and derivatives, tocopherols and derivatives, vitamin A and derivatives), 2-hydroxycarboxylic acids (for example, citric acid, malic acid, L-, Dou DL-lactic acid), colorants skin (for example, nut extracts or dihydroxyacetone), active ingredients to promote hair growth (for example, minoxidil, difenciprone, hormones, caffeine, fin asterides, phytosterols, such as beta-sitosterol, biotin, or extracts of Cimicifuga racemosa, Eugenia caryophyllata or Hibiscus rosa-sinensis, barley, hops, or rice or wheat hydrolyzates), skin care products (eg cholesterol, ceramides, pseudoceramides), softening, moisturizing and / or moisture-maintaining substances (eg glycerol or urea), fats, oils, saturated fatty acids, monounsaturated or polyunsaturated fatty acids, alpha hydroxy acids, polyhydroxy acids fatty acids or their derivatives (for example, linoleic acid, alpha-linolenic acid, gamma-linolenic acid or arachidonic acid and their respective natural or synthetic esters), waxes or other conventional constituents of a cosmetic or dermatological formulation, such as alcohols, polyols, polymers, foam stabilizers, electrolytes, organic solvents, silicone derivatives or chelating agents (eg ethylene diaminetetraacetic acid and derivatives), anti-inflammatory substances aspa (for example, climbazole, ketoconazole, pyroctonoleamine, zinc pyrithione), hair care products, perfumes, antifoams, dyes, pigments with a coloring action, thickeners (advantageously silicon dioxide, aluminum silicates, such as bentonites, polysaccharides or their derivatives, for example hyaluronic acid, fine flour from guar seed, xanthan gum, hydroxypropyl methyl cellulose or allulose derivatives, particularly and advantageously polyacrylates, such as carbopoles, or polyurethanes), surfactants, emulsifiers, plant parts or plant extracts (for example, arnica, aloe, beard lichen, ivy, nettle, ginseng, henna, chamomile, marigold, rosemary, sage, horsetail or thyme), animal extracts, for example, royal jelly or propolis, proteins, protein hydrolysates, yeast extracts, hops and wheat extracts, peptides or thymus extracts.
[0178] [000178] Among the "preservatives" (additional preservatives) for cosmetic preparations, the following can be mentioned: benzoic acid, formic acid and its sodium salt; propionic acid, salicylic acid, sorbic acid, other weak acids, for example, free fatty acids, esters and their derivatives, undec-10-enoic acid and its salts; formaldehyde, including for formaldehyde; biphenyl-2-ol and salts thereof; pyrithione zinc; inorganic sulfites and hydrogen sulfites; chlorobutanol; 4-hydroxybenzoic acid and its salts and esters; 3-acetyl-6-methylpyran-2,4 (3H) -dione (dehydracetic acid) and its salts; 3,3'-dibromo-4,4'-hexamethylenodioxidibenzamidine (dibromohexamidine) and its salts (including isethionate); thiomersal; fernylmercuric salts (including borate); hexetidine; 5-bromo-5-nitro-1,3-dioxane; bronopol; 2,4-dichlorobenzyl alcohol; triclocarbon; 4-chloro-m-cresol; triclosan; 4-chloro-3,5-xylenol; 3,3'-bis (1-hydroxymethyl-2,5-dioxoimidazolidin-4-yl) -1,1'-methylenediurea (imidazolidinyl urea); poly (1-hexamethylenebiguanide hydrochloride; 2-phenoxyethanol; hexamethylenetetramine (methenamine); methenamine 3-chloroaloylochloride; 1- (4-chlorophenoxy) -1- (imidazol-1-yl) -3,3-dimethylbutan-2-one ; 1,3-bis (hydroxymethyl) -5,5-dimethylimidazolidine-2,4-dione; benzyl alcohol; 1-hydroxy-4-methyl-6 (2,4,4-trimethylpentyl) 2-pyridone and its salt monoethanolamine; 6,6-dibromo-4,4-dichloro-2,2'-methylenediphenol (bromochlorophene); 4-isopropyl-m-cresol; mixture of 5-chloro-2-methyl-isothiazole-3 (2H) -one and 2-methylisothiazole-3 (2H) -one with magnesium chloride and magnesium nitrate; 2-benzyl-4-chlorophenol (chlorophene); 2-chloroacetamide; chlorhexidine and its diglytonate, diacetate and dihydrochloride; 1-phenoxypropan-2- ol; (C12-C22) alkyl bromide trimethyl ammonium; 4,4-dimethyl-1,3-oxyzalidine; N- (hydroxymethyl) -N- (dihydroxymethyl-1,3-dioxo-2,5-imidazoli- dinyl-4) -N '- (hydroxymethyl) urea and chloride; 1,6-Di- (4-amidinophenoxy) -n-hexane (Hexamidine) and its salts (including isethionate and p-hydroxybenzoate); glutaraldehyde (pentane - 1.5-dial); 5-ethyl-3,7-dioxa-1-azabicyclo [3.3.0] octane; 3- (p-chlorophenoxy) -propane-1,2 diol (chlorphenesin); hydroxymethylamino sodium acetate (sodium hydroxymethylglycinate); silver chloride deposited on titanium dioxide; benzethonium chloride; benzalkonium chloride, bromide and saccharate; benzyl-hemiformal; iodopropynyl butylcarbamate (IPBC) 3-iodo-2-propynylbutylcarbamate; methylisothiazolinone, sodium hexametaphosphate, ethylenediaminetetraacetic acid, peptides such as polylysine, lauric arginate, cultured dextrose, neem oil, eugenol, p-cymene, thymol, carvacrol, linalool, hydroxycinnamic acid, cinnamic acid, melanic acid, melamine common foxglove extract, açaí powder, 4-hydroxybenzyl isothiocinate and / or white mustard seed essential oil, ferulic acid, or mixtures of two or more of these.
[0179] [000179] The other preservative agents, such as 1,3-diols, which are mentioned in WO 2011/023582, and / or benzaldehydes, such as those disclosed in WO 2009/000097, or the Scutellaria baicalensis extracts, such like those disclosed in KR 20030012821, are also understood as preservatives. Scutellaria baicalensis extracts comprising dimethoxytetrahydroxyflavone and / or baicalein (5,6,7-trihydroxyflavone) are preferred, for example, as obtainable by extraction with a solvent selected from propylene glycol, glycerin, 1,3- butylene glycol, water, ethanol, and mixtures thereof.
[0180] [000180] The cosmetics according to the invention preferably comprise only natural preservatives, or no preservatives are added in view of the preservative properties of the compound (s) of formula I.
[0181] [000181] The invention also encompasses cosmetics, especially their use, which comprise, in addition to one or more of the compounds of formula I, also "other antimicrobial active agents, natural", for example, proteins, corresponding peptides, alone or in combination. combination, natural essential oils or their derivatives, such as anileira oil, lemon, orange, grapefruit, rosemary, thyme, lavender, tea tree, cider, wheat, lemongrass, cedar, cinnamon, eucalyptus, peppermint, basil, herb - sweet, menthol, Ocmea origanum, Hydastis carradensis, Krameria lappacea, Podophyllum spp., Curcuma longa, or mixtures of two or more such oils.
[0182] [000182] In certain embodiments of the invention, essential oils are used in combination with emollient solvents and AHAs. Essential oils ("EOs"), as defined in this document, are volatile oils obtained from plant or animal sources, or their synthetic equivalents, and are composed of complex mixtures of different constituents, such as monoterpenes and hydrocarbons of sequiterpenes, monoterpene and sesquiterpene ; alcohols, esters, ethers, aldehydes, ketones, oxides and the like. Examples of EOs include, but are not limited to: bergamot oil, clarion oil, sage oil, almond oil, ylang-ylang oil, neroli oil, sandalwood oil, frankincense oil, ginger oil, peppermint oil, lavender oil, absolute jasmine, geranium oil bourbon, mint oil, clove oil, patchouli oil, rosemary oil, rosewood oil, sandalwood oil, oil tea tree oil, vanilla oil, lemongrass oil, cedar oil, balm oils, tangerine oil, Japanese cypress oil, Hiba oil, ginko oil, eucalyptus oil, lemon oil, orange oil, thyme oil, savory oil, oregano oil, and lime orange oil. Botanists, such as camphor and cinnamon, can also be used. The individual constituents ("ICs") of essential oils can be natural or wholly or partly synthetic, and include, but are not limited to, l-citronellol, alpha-amylcinamaldehyde, liral, geraniol, famesol, hydroxycitralal, isoeugenol, eugenol, eucaliptol, linalool, citral, thymol, limonene and menthol. In addition, sequiterpenoids, such as nerolidol, farnesol, bisabolol and apritone, can also be used in the present invention. Mixtures of one or more EOs, one or more ICs, and one or more EOs, as well as one or more ICs, are included by the present invention.
[0183] [000183] Possible UV filters include, but are not tetrasulfonic; 2-phenylbenzimidazole-5-sulfonic acid and corresponding salts; 1,4-di (2-oxo-10-sulfo-3-bornylidenmethyl) -benzene and corresponding salts; 4- (2-oxo-3-bornylidenmethyl) benzenes-ulfonic acid and its salts; 2-methyl-5- (2-oxo-3-bornylidenmethyl) sulfonic acid and its salts; 2,2'-methylen-bis- (6- (2H-benzotriazol-2-yl) -4- (1,1,3,3-tetrame-tilbutyl) -phenol); 2- (2H-benzotriazol-2-yl) -4-methyl-6- [2-methyl-3- [1,3,3,3-tetr-amethyl-1 - [(trimethylsilyl) oxy] disyloxanil] propyl] -phenol; 3- (4-methylbenzyliden) camphor; 3-benzylidencamphor; 4- (tert-butyl) -4'-methoxydibenzoylmethane; 2- (4'-diethylamino-2'-hydroxybenzoyl) -benzoic acid methyl ester; tereftalidendicanforasulfonic acid; 4- (dimethylamino) -benzoic acid (2-ethylhexyl) ester; 4- (dimethylamino) benzoic acid amyl ester; 4-ethoxybenzalmalonic acid ester (2-ethylhexyl); 2-hydroxy-4-methoxybenzophenone, 2-hydroxy-4-methoxy-4'-methylbenzophenone; 2,2'-dihydroxy-4-methoxybenzophenone; 2-ethylhexyl-2-hydroxybenzoate; 3- (4- (2,2-bis ethoxycarbonylvinyl) -phenoxy) propenyl) -methoxysiloxane / dimethylsiloxane copolymer; dioctylbutylamidotriazone (INCI: Diethylhexyl-Butamidotriazone); 2,4-bis- [5-1 (dimethylpropyl) benzoxazol-2-yl- (4-phenyl) -imino] -6- (2-ethylhexyl) -imino-1,3,5-triazine (CAS RN 288254-16-0); 4,4 ', 4 "- (1,3,5-triazin-2,4,6-triyltriimino) -tris-benzoic acid tris (2-ethylhexylester) (also: 2,4,6-tris- [anilino- (p-carbo-2'-ethyl-1'-hexyloxy)] - 1,3,5-triazine (INCI: Ethylhexyl Triazone); 2,4-bis - {[4- (2-ethyl -hexyloxy) -2-hydroxy] -phenyl} -6- (4-methoxyphenyl) -1,3,5-triazine (INCI: Bis-Ethyl-hexyloxyphenol Methoxy-phenyl Triazine); 2,4,6-tris- ( biphenyl) -1,3,5-triazine; 2,4-bis- (4'-di-neopentylaminobenzalmalonate) -6- (4 "-butylaminobenzoate) -s-triazine, 4-dicianomethylen-2,6-dimethyl-1 , 4-dihydropyridin-N- (ethyloxysulfate ester salt), titanium dioxides, zinc oxides, merocyanine, piperazine derivatives as mentioned in WO 2011/042088, without being limited thereto.
[0184] [000184] Possible solvents include, but are not limited to: alcohols, such as methanol, ethanol, butanol, pentanol (amyl alcohol), ethylene glycol, propylene glycol, glycerol, butyl acetate, dimethyl sulfoxide, acetone, methyl ethyl ketone, hydrocarbons, such as hexane, pentane, oils, such as zea mays oil, or the like.
[0185] [000185] Cosmetics can be solid, for example, of a waxy or similar aspect, or liquids or be in the form of pastes or creams, for example, as emulsions, solutions or suspensions, for example, mixtures of oil in water or water in oil (O / W or W / O). They can thus form, for example, a solution, a water-in-oil (W / O) or oil-in-water (O / W) emulsion, or a multiple emulsion, for example, water-in-oil type in water (W / O / W), a gel, a hydrodispersion, a solid stick or an aerosol.
[0186] [000186] Possible surfactants include, but are not limited to, usual, for example, anionic, non-ionic, amphoteric surfactants, such as soaps or sodium dodecyl sulfate, or the substances disclosed in WO 2011/023582.
[0187] [000187] It is to be mentioned here that, in view of their molecular structures, the compounds of formula I can also contribute to the properties of surfactants for a composition according to the invention, so that their use is a preferred modality of invention.
[0188] [000188] The composition comprising one or more compounds of the formula I according to the invention can be applied to the skin or lips or other surfaces of the body, for example, hair, nails or teeth, according to the use for the whatever it is intended. It can therefore be used in a method for the cosmetic treatment of said body surfaces, for example, the skin, comprising applying the composition according to the invention to said body surface, for example, to the skin, for example. example, for the purpose of strengthening it, regenerating it or smoothing, for example, its wrinkles on the skin and / or to combat aging, for example, of the skin, or the harmful effects of UV radiation and / or to fortify skin tissues, teeth, hair and / or nails against environmental attacks.
[0189] [000189] In an alternative form, the composition according to the invention can be used for the manufacture of a dermatological preparation. Home care products
[0190] [000190] As possible home care products to be provided with one or more of the compounds of formula I, among others, detergents for washing clothes, detergents for washing dishes, fabric softeners can be mentioned , strong surface cleaner or bleaching compositions; surface cleaners, clothes and / or dishes, soaps for washing clothes, air fresheners and odor eliminators, insect repellents, detergents for washing clothes, fabric softeners, bleaching agents , organic cleaners, grease removers, stain removers, window and glass cleaners, bathroom and toilet cleaners, floor cleaners, carpet cleaners, pet odor removers, deodorants for cat boxes, car coolers, furniture shine, waterless hand cleaners, disinfectants, spray deodorants, food processing plant cleaners, coloring materials or other similar care applications House.
[0191] [000191] Home care products have usual compositions. For example, in addition to surfactants, solvents, dyes, preservatives, emulsifying agents, perfumes, antibacterial agents, thickeners, conditioners, antistatic agents, silicone surfactants, and other similar conventional ingredients may be included. that are typically present in conventional home care formulations. The mixtures and / or combinations of the additional formulation agents mentioned above can also be employed in the present invention. The amounts of the additional formulation agents that can be employed in the present invention are within ranges that are well known to those skilled in the art and the further formulation is carried out using processes that are also well known in the art. Pharmaceutical substances
[0192] [000192] Pharmaceutical substances comprise one or more pharmaceutically active agents and a carrier material pharmaceutically acceptable.
[0193] [000193] Examples of such pharmaceutical substances (pharmaceutical compositions) are, for example, solid formulations (tablet, capsule, powder, medical chewing gum, lozenge, suppository) or liquid (e.g. injection solution, infusion solution , syrup, drinkable solution), a spray, or a pasty material, for example, a gel or cream.
[0194] [000194] Among the possible active ingredients, all the drugs known in the art can be added, for example, (without this enumeration being intended to be limiting) bronchodilators, antipyretics, analgesics, antipyretics, antiarrhythmic agents, reducing agents blood pressure, vasodilators, anticholinergics, anti-arteriosclerotic agents, enzymes, antibodies, secretolytics, ulcer preparations, antiproliferative agents, vasoconstrictors, expectorants, antitussives, mucolytics, or secretomotor drugs; in particular, antiallergic-free (including those mentioned here before), such as α-sympathometics (in particular, Phenylephrine, Ephedrine, Tetrizoline, Naphazoline, Oxymetzoline, Xylometazoline or Tramazoline), antihistamines, non-steroidal or anti-inflammatory active substances steroids (in particular, Triamcinolone acetonide, glucocorticoids, such as Prednisolone, Triamcinolone acetonide, Clometasone, Dexamethasone, or Fluticasone), β2 sympathomimetics; mast cell stabilizers, aromatase inhibitors; antiestrogens; topoisomerase I inhibitors; topoisomerase II inhibitors; active microtubule compounds; alkylating compounds; histone deacetylase inhibitors; compounds that induce cell differentiation processes; cyclooxygenase inhibitors; MMP inhibitors; mTOR inhibitors; antineoplastic antimetabolites; platinum compounds; compounds that target / decrease protein or lipid kinase activity and additional antiangiogenic compounds; compounds that target, decrease or inhibit the activity of a protein or lipid phosphatase; gonadorelin agonists; antiandrogens; methionine aminopeptidase inhibitors; bisphosphonates; biological response modifiers; antiproliferative antibodies; heparanase inhibitors; inhibitors of Ras oncogenic isoforms; telomerase inhibitors; proteasome inhibitors; compounds used to treat hematological malignancies; compounds that target, decrease or inhibit Flt-3 activity; Hsp90 inhibitors, such as 17-AAG (17-allylamino-geldanamycin, NSC330507), 17-DMAG (17-dimethylaminoethylamino-17-demethoxy-geldanamycin, NSC707545), IPI-504, CNF1010, CNF2024, CNF1010 from Conforma Therapeutics; temozolomide (TEMODAL®); kinesin spindle protein inhibitors, such as GlaxoSmithKline SB715992 or SB743921, or CombinatoRx pentamidine / chlorpromazine; MEK inhibitors, such as ARRY142886 from Array PioPharma, AZD6244 from AstraZeneca, PD181461 from Pfizer, leucovorin, EDG binders, antileukemia compounds, ribonucleotide reductase inhibitors, S-adenosylmethionine decarboxylase inhibitors, or other antiproteins; tricyclics, for example, benzodiazepines, including MAO inhibitors of mitochondrial benzodiazepine binders, SSRI's, SNRI's, NK receptor antagonists, CRF receptor antagonists, 5HT7 receptor antagonists, mGlu receptor agonists / antagonists / modulators, agonist / antagonists or modulators of the GABA-A or GABA-A / B receptor, vasopressin receptor antagonists, phytomedication, such as St. John's wort, 5-HT1A receptor agonists, vasopressin receptor antagonists, acetylcholine esterase inhibitors, such as rivastigmine or donepezil, mixed acetylcholine / butyrylcholine esterase inhibitors, nicotinic-alpha7 agonists, typical or atypical antipsychotics, such as clozapine or haloperidol, nicotinic-alpha7 agonists, eg anti-manic agents lithium, Carbamazepine, Valproate) or any atypical or typical antipsychotic; or similar; the pharmaceutically acceptable salts thereof, if salt-forming groups are present, or the combinations of two or more of the aforementioned active substances or the pharmaceutically acceptable salts thereof.
[0195] [000195] Pharmaceutical compositions comprising one or more active ingredients and one or more compounds of the formula I, in association with at least one acceptable pharmaceutical carrier or diluent, can be manufactured in the conventional manner by mixing with a pharmaceutically acceptable carrier or diluent.
[0196] [000196] The invention also relates to pharmaceutical compositions comprising an antimicrobially effective amount, especially an amount effective in treating one of the above mentioned disorders, one or more compounds of formula I, a pharmaceutically acceptable salt thereof, and / or a its ester, together with one or more pharmaceutically acceptable carriers which are suitable for topical, enteral, for example, oral or rectal, or parenteral administration and which can be inorganic or organic, solid or liquid. Especially tablets or gelatin capsules comprising the active ingredient together with diluents, for example, lactose, dextrose, mannitol, and / or glycerol, and / or lubricants and / or polyethylene glycol, may be used for oral administration. The tablets may also comprise binders, for example, aluminum magnesium silicate, starches, such as corn, wheat or rice starch, gelatin, methylcellulose, sodium carboxymethylcellulose and / or polyvinylpyrrolidone, and, if desired, disintegrants, for example, starches, agar, alginic acid or a salt thereof, such as sodium alginate, and / or effervescent mixtures, or adsorbents, dyes, flavors and sweeteners. It is also possible to use the pharmacologically active compounds of the present invention in the form of parenterally administrable compositions or in the form of infusion solutions. The pharmaceutical compositions can be sterilized and / or can comprise excipients, for example, preservatives, stabilizers, wetting and / or emulsifying compounds, solubilizers, salts for regulating osmotic pressure and / or buffers. The present pharmaceutical compositions, which may, if desired, comprise other pharmacologically active substances, are prepared in a manner known per se, for example, by means of conventional mixing, granulating, confectioning, dissolving or lyophilizing processes, and comprise approximately 1% to 99% by weight, especially from approximately 1% to approximately 60% of active ingredient (s) and 0.001 to 10, 0.01 to 8, 0.02 to 6 or 0.03 to 5 percent by weight of the compound (s) of the formula I, a pharmaceutically acceptable salt and / or an ester thereof. Also included is the use of its preservative or antimicrobial properties in said pharmaceutical compositions by adding one or more compounds of formula I.
[0197] [000197] Additives, both in the case of food and cosmetics, as well as in the case of pharmaceutical substances (the term pharmaceutical substances also including nutraceuticals), may exhibit more than one property as selected from the aforementioned or other lists properties not mentioned, for example, preservatives can also act as acidity regulators and vice versa, or, for example, antioxidants can act as preservatives as well as acidity regulators, or other multifunctional uses imaginable. Medical Devices
[0198] - diagnóstico, prevenção, monitoramento, tratamento ou alívio da doença, - diagnóstico, monitoramento, tratamento, alívio de, ou compensação por, uma lesão ou deficiência física, - investigação, reposição ou modificação da anatomia ou de um processo fisiológico, - controle da concepção, e que não atinja a sua ação pretendida principal no, ou sobre o, corpo humano por meio farmacológico, imunológico ou metabólico, porém que possa ser auxiliado em sua função por tais meios. [000198] Medical devices are especially devices intended for use in the diagnosis of disease or other conditions, or in the cure, mitigation, treatment, or prevention of disease, in man or other animals, and mean, for example, any instrument, apparatus, utensil, material or other article, whether used alone or in combination, including the software necessary for its appropriate application, intended by the manufacturer to be used for human beings for the purpose of: - diagnosis, prevention, monitoring, treatment or relief of the disease, - diagnosis, monitoring, treatment, relief from, or compensation for, an injury or physical disability, - investigation, replacement or modification of the anatomy or a physiological process, - control of conception, and that does not achieve its main intended action on, or on, the human body through pharmacological, immunological or metabolic means, but that can be aided in its function by such means.
[0199] [000199] Among medical devices, among others, for example, mention may be made of implants, prosthesis, plasters (= adhesive tapes), dressing materials (from wounds), bandages, cotton, gas bandages, surgical instruments, toothbrushes, syringes, syringe needles, medication containers, infusion bottles, infusion tubes, valves or multiple compartments used for infusion, infusion needles, infusion, surgical instruments, catheters, artificial or natural tissues or membranes, toothbrushes, and the like.
[0200] a) Malhas cirúrgicas ou outros materiais bidimensionalmente estendidos ou estendíveis (tais como as membranas), tais como as malhas de polipropileno (por exemplo, BARD MESH® da Bard Inc., SURGIPRO® da US Surgical, Inc., TRELEX® da Boston Scientific, PROLENE® ou MERSILENE® da Ethicon, Inc.), as malhas de poliéster (por exemplo, MERSILENE® da Ethicon), as malhas de politetrafluoretileno expandidas (por exemplo, SOFT TISSUE PATCH® da W.L. Gore & Associates, Inc), os materiais de poliamida ou similar, por exemplo, para a restauração de hérnias; ou tais malhas ou materiais bidimensionalmente estendidos ou estendíveis, tais como a poliglactina (por exemplo,VICRYL® da Ethicon, Inc.), o poliglicolato (por exemplo, DEXON® da US Surgical, Inc.), a polidioxanona (PDS), o poligliconato (por exemplo, MAXON®, Davis & Geck, Gosport, UK) ou os materiais de colágeno, por exemplo, COOK SURGISIS® da Cook Biomedical, Inc.; os outros materiais possíveis de membrana ou malha incluem FLUORO-TEX® Pericardial and Peritoneum Surgical Membrane ou FLUORO-TEX Dura Substitute (cada um da C.R. Bard) ou PRECLUDE® Pericardial Membrane PRECLUDE® Peritoneal Membrane e PRECLUDE® Dura Substitute Membrane, cada um da W.L. Gore & Associates, os elastômeros de silicone, tais como SILASTIC Rx® Medical Grade Sheeting da Dow Corning ou Polipropileno microporoso da Celgard, Inc., por exemplo, onde o implante for usado para vedar tecidos ou estruturas de compartimentação no corpo, tais como o peritônio, a pleura, o diafragma, o pulmão, o pericárdio ou similar; b) revestimentos de eletrodos, por exemplo, para eletrodos de marca-passos ou estímulo neural ou muscular ou similar, por exemplo, feitos de tungstênio, silício, platina-irídio ou aço inoxidável ou combinações dos mesmos; c) implantes de ossos ou cartilagem degradáveis ou não degradáveis; d) implantes ortopédicos, tais como implantes que substituem o tecido duro, o osso ou a articulação, por exemplo, para quadril ou joelho ou outra restauração de articulação, por exemplo, implantes feitos de aço inoxidável, ligas de cobalto-cromo, titânio ou ligas de titânio, titânio puro, tântalo, materiais plásticos, tais como polietileno, polipropileno, polilactato, fibra de carbono, cerâmica ou compostos de dois ou mais tais materiais; e) parafusos, pregos, roscas, placas ou outros materiais de fixação duros para os tecidos duros, por exemplo, a partir dos materiais mencionados em d); f) implantes orais, tais como dentais, por exemplo, a partir dos materiais mencionados em d); g) Enchimentos de ossos, tais como os compósitos de cimentos de ossos, os compósitos de hidroxil apatita ou a policaprolactona (recheio de Blurr); h) implantes que entram em contato com o sangue, tais como os enxertos vasculares, por exemplo, a partir de materiais plásticos biocompatíveis, tais como o politetrafluoretileno ou o poli[tereftalato de etileno] estendido, os stents (por exemplo, de metais ou ligas de metais, tais como o ácido inoxidável (por exemplo, 316L), a liga de cobalto-cromo-níquel-molibdênio-ferro, o Tântalo, as ligas com memória de forma, por exemplo, o nitinol, ou os materiais de polímeros (por exemplo, com memória de forma), tais como o polietileno ou a poliuretana), as válvulas cardíacas ou venosas (por exemplo, a partir de materiais de polímeros ou metais ou naturais ou suas combinações, por exemplo, o carbono pirolítico, o titânio revestido com o carbono pirolítico, e o manguito de anel de costura é, por exemplo, o teflon, o poliéster ou o dácron), as combinações de stent/válvula, ou os acessos contínuos, por exemplo, às veias ou ao peritônio, por exemplo, para a diálise peritoneal ou similar; i) implantes para a liberação de sinais ou substâncias químicas, por exemplo, fármacos, que entram em contato com o tecido e/ou os fluidos do corpo, por exemplo, bombas para a liberação de fármacos ou marca-passos; j) órgãos ou tecidos para a transplantação (por exemplo, para diminuir a expressão de antígenos que despertam a rejeição ao transplante), especialmente os autoenxertos, os aloenxertos, os heteroenxertos ou os xenoenxertos; k) substitutos da pele ou materiais de revestimento de feridas, tais como naturais (por exemplo, ceratinócitos em combinação com fibroblastos humanos no colágeno bovino do tipo I ou outras proteínas de ECM e citocinas, tais como Apligraf® (Organogenisis Inc.)), ou a partir de sintéticos, ou as combinações com materiais naturais, por exemplo, polissiloxanos sintéticos com colágeno bovino do tipo I e condroitina-6-sulfato (por exemplo, Integra® (Johnson & Johnson Medica Care Life)) ou preferivelmente a partir de sintéticos sozinhos ou combinados com o tecido dérmico humano (por exemplo, Tanscyte® (Advanced Tissue Sciences Inc.)), aloenxertos, colágeno (por exemplo, na forma reconstituída) ou similar; l) materiais de sutura (especialmente para as suturas internas não acessíveis a partir do lado de fora), por exemplo, a partir de sintéticos ou materiais naturais (por exemplo, intestino do gato) absorvíveis ou não absorvíveis. [000200] Possible implants include, but are not limited to: a) Surgical meshes or other two-dimensionally extended or stretchable materials (such as membranes), such as polypropylene meshes (for example, BARD MESH® by Bard Inc., SURGIPRO® by US Surgical, Inc., TRELEX® by Boston Scientific , PROLENE® or MERSILENE® by Ethicon, Inc.), polyester meshes (for example, MERSILENE® by Ethicon), expanded polytetrafluoroethylene meshes (for example, SOFT TISSUE PATCH® by WL Gore & Associates, Inc), polyamide or similar materials, for example, for hernia restoration; or such two-dimensionally extended or extendable meshes or materials, such as polyglactin (eg VICRYL® by Ethicon, Inc.), polyglycolate (eg DEXON® by US Surgical, Inc.), polydioxanone (PDS), polyglycolate (for example, MAXON®, Davis & Geck, Gosport, UK) or collagen materials, for example, COOK SURGISIS® by Cook Biomedical, Inc .; other possible membrane or mesh materials include FLUORO-TEX® Pericardial and Peritoneum Surgical Membrane or FLUORO-TEX Dura Substitute (each from CR Bard) or PRECLUDE® Pericardial Membrane PRECLUDE® Peritoneal Membrane and PRECLUDE® Dura Substitute Membrane, each of each WL Gore & Associates, silicone elastomers such as SILASTIC Rx® Medical Grade Sheeting from Dow Corning or microporous polypropylene from Celgard, Inc., for example, where the implant is used to seal tissues or compartmental structures in the body, such as the peritoneum, the pleura, the diaphragm, the lung, the pericardium or similar; b) electrode coatings, for example, for pacemaker electrodes or neural or muscular stimulus or similar, for example, made of tungsten, silicon, platinum-iridium or stainless steel or combinations thereof; c) degradable or non-degradable bone or cartilage implants; d) orthopedic implants, such as implants that replace hard tissue, bone or joint, for example, for hip or knee or other joint restoration, for example, implants made of stainless steel, cobalt-chromium alloys, titanium or titanium alloys, pure titanium, tantalum, plastic materials, such as polyethylene, polypropylene, polylactate, carbon fiber, ceramics or compounds of two or more such materials; e) screws, nails, threads, plates or other hard fastening materials for hard fabrics, for example, from the materials mentioned in d); f) oral implants, such as dental implants, for example, from the materials mentioned in d); g) Bone fillers, such as bone cement composites, hydroxyl apatite composites or polycaprolactone (Blurr filling); h) implants that come into contact with blood, such as vascular grafts, for example, from biocompatible plastic materials, such as polytetrafluoroethylene or extended poly [ethylene terephthalate], stents (for example, metals or metal alloys, such as stainless acid (eg 316L), cobalt-chromium-nickel-molybdenum-iron alloy, tantalum, shape memory alloys, eg nitinol, or polymer materials (for example, with shape memory), such as polyethylene or polyurethane), heart or venous valves (for example, from polymer or metal or natural materials or their combinations, for example, pyrolytic carbon, titanium coated with pyrolytic carbon, and the seam ring cuff is, for example, teflon, polyester or dacron), stent / valve combinations, or continuous access, for example, to the veins or peritoneum, for example, for peritoneal dialysis or the like; i) implants for the release of signals or chemical substances, for example, drugs, which come into contact with tissue and / or body fluids, for example, pumps for the release of drugs or pacemakers; j) organs or tissues for transplantation (for example, to decrease the expression of antigens that trigger transplant rejection), especially autografts, allografts, heterografts or xenografts; k) skin substitutes or wound lining materials, such as natural (for example, keratinocytes in combination with human fibroblasts in bovine type I collagen or other ECM proteins and cytokines, such as Apligraf® (Organogenisis Inc.)), either from synthetics, or combinations with natural materials, for example, synthetic polysiloxanes with type I bovine collagen and chondroitin-6-sulfate (eg Integra® (Johnson & Johnson Medica Care Life)) or preferably from synthetic alone or combined with human dermal tissue (for example, Tanscyte® (Advanced Tissue Sciences Inc.)), allografts, collagen (for example, in reconstituted form) or similar; l) suture materials (especially for internal sutures not accessible from the outside), for example, from synthetic or natural materials (eg cat gut) absorbable or non-absorbable.
[0201] [000201] Artificial implants made of metals, metal alloys, synthetic materials (= polymers) (degradable or non-degradable), carbon fibers, boranes, ceramics, replacement materials for glass or bone, especially types a ) to i) or k) to l) mentioned above, including composites of two or more such materials.
[0202] [000202] Implants can be for permanent insertion (for example, in the case of joint replacement) or transient (for example, in the case of fixation devices or skin replacements) or other administration.
[0203] [000203] Especially here, the usefulness of inhibiting biofilms of the compound (s) of formula I and the compositions which they comprise is of benefit. Active Packaging Materials
[0204] [000204] Among the active packaging materials, for example, a food or beverage or pharmaceutical or surgical packaging material that has a preventive / preservative effect against deterioration, for example, against colonization by bacterial films or other micro- organisms or against the deterioration of materials that come into contact with other perishable products, can be mentioned, for example, cans, covers, foil, bottles, canons, cardboard boxes, bathtubs, bags, cartridges, tubes, sachets, ampoules, bags, or the like.
[0205] [000205] Regarding both medical devices and active packaging materials, as well as personal care products used having a predetermined format, the application of the compound (s) of formula I is especially by coating, for example, on surfaces that come into contact with perishable products or a human or animal, or mass integration (for example, by mixing the starting materials and / or impregnating the final products) into the material.
[0206] [000206] The compound (s) of the formula I for medical devices and active packaging materials can be specially applied (alone) or in combination with appropriate carrier materials) on surfaces that come into contact with contact with perishable products or a human or animal, for example, in the form of a coating, or applied (s) by mass integration into the material.
[0207] [000207] The materials provided (which form an embodiment of the invention) or to be provided with one or more compounds of formula I may comprise the compound (s) of formula I, a physiologically acceptable salt thereof and / or one thereof ester, mixed with the mass of the material, or (in the case of products with a stable surface) by covalent and / or non-covalent bonding to (part or all of) said surface.
[0208] [000208] For covalent bonding, the surface must expose or be chemically modified to expose the functional groups that would allow the covalent bonding of the compound of formula I directly or by means of a spacer molecule.
[0209] [000209] In the case of covalently linked (at least bivalent) binding molecules, these may allow the covalent or non-covalent bonding of the compounds of formula I.
[0210] [000210] The linker in the covalent linkage method can be any linker.
[0211] 1. As superfícies funcionalizadas com epóxi ou éster ativado, onde é possível a reação com os grupos OH ou amino nos precursores ligadores. 2. Onde os precursores ligadores forem compostos orgânicos, os quais são fornecidos na extremidade com um grupo tiol, eles podem ser ligados, por exemplo, por meio de superfícies folheadas a ouro ou superfícies com camadas de maleinimida. 3. Os precursores ligadores que, durante o processo de fabricação, forem fornecidos na extremidade com um grupo carboxila ou fosfato, podem ser ativados até ésteres ativos ou similares, por exemplo, com EDC, de modo que um OH, SH ou amino reativo sobre a superfície possa ser ligado. 4. Os precursores para reticulador homo- ou, preferivelmente, hetero-bifuncional que podem ser ligados a grupos reativos na superfície, tais como grupos carboxila, epóxi, OH, SH, aldeído ou amino; ou outros métodos. 5. Outras superfícies diretamente funcionalizadas, por exemplo, especialmente os polímeros com aldeídos revestidos com plasma. [000211] The covalent bonding of compounds of formula I with or without linkers can occur directly by reaction of their precursors with surfaces, without activation, or to surfaces activated on implants or other products. Examples are: 1. Surfaces functionalized with epoxy or activated ester, where it is possible to react with OH or amino groups in the binding precursors. 2. Where the precursor binders are organic compounds, which are provided at the end with a thiol group, they can be linked, for example, by means of gold-plated surfaces or surfaces with layers of maleinimide. 3. Binding precursors that, during the manufacturing process, are supplied at the end with a carboxyl or phosphate group, can be activated until active or similar esters, for example, with EDC, so that an OH, SH or reactive amino on the surface can be connected. 4. The precursors for homo- or, preferably, hetero-bifunctional crosslinker that can be attached to reactive groups on the surface, such as carboxyl, epoxy, OH, SH, aldehyde or amino groups; or other methods. 5. Other directly functionalized surfaces, for example, especially polymers with plasma-coated aldehydes.
[0212] [000212] Other possible activations for both covalent and non-covalent bonding include, but are not limited to, luminescent discharge surfaces, electrostatically charged surfaces, and / or rough surfaces. Also coating with materials, for example, gels, varnishes, paints or the like, which comprise the compound (s) of the formula I is a method for providing their surfaces with these compounds.
[0213] [000213] In the (preferred) case of the non-covalent bond, the material can be any substrate. This substrate could be a synthetic polymer (ie, polyacrylate, polylactide-co-glycolide, polyethylene, or polypropylene), carbon fiber, glass, boranes, metal (ie, titanium or stainless steel), natural polymer (ie, collagen or alginate), or any other surface that is capable of supporting a coating, for example, in the form of solid or fiber, respectively. Composites of two or more such materials are also included. The non-covalent can, for example, be through adsorption, integration into a coating matrix or the like.
[0214] [000214] The compounds of the formula I in the modalities of the invention, in an additional modality, can also be used where the materials (products) with which they are associated (for example, by mixing) require a heat treatment, for example, for obtain sterilization by pasteurization.
[0215] [000215] Thus, in one embodiment, the one or more compounds of formula I are heat-stable. In one example, the compound (s) of the formula I totally or partially conserves the structure and activity, for example, in relation to its preservative properties after heating. Heating of the antimicrobial composition can be carried out at 60-130 ° C, such as in the range of 60-65 ° C, 65-70 ° C, 70-75 ° C, 75-80 ° C, 80-85 ° C , 85-90 ° C, 90-95 ° C, 95-100 ° C, 100 -105 ° C, 105-110 ° C, 110-115 ° C, 115-120 ° C, 120-125 ° C, 125 -130 ° C.
[0216] [000216] In one embodiment, heating is carried out at around 65-75 ° C, more preferred at around 70 ° C.
[0217] [000217] In one embodiment, heating is carried out at around 90-110 ° C, more preferred at around 100 ° C.
[0218] [000218] In yet another modality, heating is carried out at around 120-125 ° C, more preferred at around 121 ° C.
[0219] [000219] According to the present invention, heating can be carried out for shorter or longer periods of time, such as from one minute to several hours. Heating can, for example, be done for a few minutes, such as in the range of about 1-5 minutes, 5-10 minutes, 10-15 minutes, 15-20 minutes, 2025 minutes, 25-30 minutes, 30 minutes - up to 1 hour.
[0220] [000220] For the purpose of the invention, other known preservative agents or known preservatives can be added to the pharmaceutical substance, including the nutraceutical and cosmeceutical, nutritional or cosmetic product, as well as to the composition.
[0221] [000221] Preferred combinations of the compounds of the invention for use in food and drinks are with weak organic acids, combinations with sorbic acid and / or benzoic acid and their appropriate salts, or with natural preservatives are especially preferred.
[0222] [000222] Among the possible preferred combinations of the compounds of the invention for cosmetic use are combinations with C1-C4 alkyl parahydroxybenzoate or its salts, for example, methylparaben, ethylparaben, propylparaben, isopropylparaben, butylparaben, isobutylparaben and appropriate salts thereof. , benzylparaben, benzoic acid or salts, for example, sodium benzoate, N- (3-chloroaloyl) hexamine chloride, alcohols or polyols, such as ethanol, propylene glycol, benzyl alcohol or 2-phenoxyethanol, benzalkonium chloride, chloroacetamide, thimerosal, benzalkonium chloride, cetylpyridinium chloride, N- (3-chloroaloyl) hexamine chloride, formaldehyde donors, such as imidazolidinyl urea, diazolidinyl urea, or DMDM hydantoin, isothiazolinones, such as KATHON® CG, commercially & available from DA Haas, Philadelphia, Pa., Which contains an isothiazolinone replaced with chlorine (methylchloroisothiazolinone), other chlorinated aromatic compounds, such as chlorphenesin, phenoxyethan ol, vicinal diols, such as a 1,2-alkane diol or a glyceryl monoether, such as glyceryl laurate, isothiazolinone decyl glycoside compounds, such as methylisothiazolinone, for example, 2-methyl-3 (2H) isothiazolinone, propionic and its salts, undec-10-enoic acid and salts, extracts of Scutellaria baicalensis (such as, for example, available from BMB-FS, or similar), or mixtures of two or more such preservatives.
[0223] [000223] In all materials, the compound (s) of the formula I can also be used as emulsifiers, in addition to the use of their preservative properties.
[0224] [000224] The compound or compounds of formula I are preferably comprised, considering the weight or material to which it is added and the compound (s) of formula I as 100% by weight, in a relative proportion by weight from 0.00001 to 10 weight percent.
[0225] [000225] In the foods or beverages according to the invention, the compound or compounds of formula I are preferably / are added / comprised in a concentration, for example, in the range of 50 to 20000 ppm, for example, from 100 to 1000 ppm, for example, from 10 to 120 ppm, such as from 30 to 60 ppm, or, for example, from 0.1 -150 ppm, where ppm refers to parts by weight per million.
[0226] [000226] The "minimum inhibitory concentration" (MIC) is a term for which no standard period of time is routinely defined or understood. In medical fields, MIC is often used to designate the concentration of a substance that prevents the growth of a single type of microorganism in the overnight incubation, compared to a positive control without the substance. However, the rest of the scientific community has adopted the term MIC to mean any of several conditions of incubation period and degree of inhibition.
[0227] [000227] Even within the medical field, it is recognized that a MIC value developed during a 24 hour incubation period may not be the same value developed after 48 hours or longer. In other words, a substance can exhibit an observable MIC during the first 24 hours of an experiment, but it cannot exhibit any measurable MIC in relation to the positive control after 48 hours.
[0228] [000228] The following table provides some of the compounds of formula I that are of interest in the various embodiments of the present invention.
[0229] [000229] The preservative properties of the compounds of the invention, for example, of the compounds of formula I, can be evaluated according to the methods cited in the art, such as WO 2010/062548. For example, they can be determined for drinks using the method described in Example 3, in WO 2010/062548, which is incorporated herein by reference. For example, a single basic drink preparation is used to prepare each of the five tests and consists of 4% apple juice, 68 g / l of sucrose, 52 g / l of glucose, 2 g / l of fructose in portioned water, which is formulated to 90 ppm hardness with calcium chloride and magnesium chloride. A pH of 3.4 is achieved through combinations of malic acid and sodium malate for all preparations, regardless of the presence or absence of the compounds of formula I. The total combined amount of sodium malate and malic acid is almost constant , but the ratio of malic acid and malate may vary slightly, given the presence of the compound of formula I. It is relevant that the drink used for the test does not naturally contain any substance with measurable antimicrobial activity, such as in essential oils. Where required, the compound of formula I is supplemented from stock solutions prepared separately. Dimethyl dicarbonate is released via a hypodermic needle (Hamilton syringe) through the septum that seals the test vessel against moisture loss. The dimethyl dicarbonate stock solution consists of 1 ml of dimethyl dicarbonate (1.25 g) in 49 ml of 100% ethanol (25 mg / ml). Consequently, one microliter of stock contains 25 micrograms of dimethyl dicarbonate. Each of the five tests employs the same bioindicator organisms; Growth (+) versus no growth (-) is established by visual inspection or spectrophotometric (see, for example, the Examples below). The names of the organisms and their strain numbers, as well as the incubation times and details about departing from the test conditions, if any are used, are mentioned in the Examples.
[0230] [000230] Among the preferred embodiments of the invention, the following are to be mentioned:
[0231] [000231] A) The first embodiment of the invention is that defined in claim 1, or a method that comprises the use mentioned herein.
[0232] [000232] B) The said use or method is especially preferred, where the material to which the agent is added is a cosmetic, a food or a drink.
[0233] [000233] C) Another embodiment of the invention refers to the use or method according to paragraph A) or B), where in the compound of the formula I m is 3 to 5, n is 2 to 5, o is 0 or 1, p is 5 to 15 and R is a portion of the sub-formula
[0234] [000234] D) Another embodiment of the invention relates to the use or method according to paragraph A) above, wherein the compound or mixture of compounds of formula I preferably comprises at least one compound selected from of the group of compounds mentioned in table 1, or a physiologically acceptable salt thereof.
[0235] [000235] E) Another embodiment of the invention relates to the use or method according to any of the aforementioned paragraphs A) to D), wherein the compound or compounds of formula I, or a physiologically acceptable salt, or a physiologically acceptable ester thereof is added to increase stability against microorganisms.
[0236] [000236] F) Another embodiment of the invention relates to the use or method according to paragraph E) above, where the microorganism is at least one microorganism selected from the group consisting of fungus, yeast and bacteria from a drink or a food or a cosmetic.
[0237] [000237] G) Another embodiment of the invention relates to the use or method according to any of the aforementioned paragraphs A) to F), where at least one additional preservative is added.
[0238] [000238] H) Another embodiment of the invention relates to the use or method according to any of the above mentioned paragraphs A) to G), where the compound or compounds of formula I, a physiologically acceptable salt thereof, and / or an ester thereof is added in the form of an extract (this term including a precipitate) from a natural source or obtained from such an extract.
[0239] [000239] L) Another modality of the invention refers to the use or the method according to paragraph H), where the source of the extract is a Dacryopinax fungus, a Ditiola and / or a Femsjonia.
[0240] [000240] J) Another modality of the invention refers to the use or method according to paragraph H), where the source of the extract is Dacryopinax spathularia, Dacrymyces sp., Ditiola radicata, Ditiola nuda and / or Femsjonia luteo-alba (= Ditiola pezizaeformis).
[0241] [000241] K) Another embodiment of the invention relates to the use or method according to paragraph J) above, where the source of the extract is the strain of Dacryopinax spathularia FU50088, the strain of Ditiola radicata MUCL 53180, the strain of Ditiola nuda CBS 173.60 or the strain of Femsjonia luteo-alba (= Ditiola pezizaeformis) MUCL 53500.
[0242] [000242] L) Another embodiment of the invention relates to the use or method according to any of paragraphs A) to K), where the material is subjected to a heat treatment before, during or after the addition of the (s) compound (s) of formula I, a physiologically acceptable salt and / or an ester thereof, as defined in any of paragraphs A), C), D) or H) to K), especially heating the material to a temperature from 60 to 130 ° C.
[0243] [000243] M) Another embodiment of the invention relates to a compound or mixture of compounds of the formula I shown in paragraph A) or as defined in any of paragraphs C), D) or H) to L), where the portion R carries at least one hydroxyl group esterified with an acid with 3 or more carbon atoms, a physiologically acceptable salt, and / or an ester thereof.
[0244] [000244] N) Another embodiment of the invention relates to the compound or mixture of compounds of paragraph M), where the acid is a C3-C10-alkanoic acid, especially isovaleric acid; a physiologically acceptable salt, and / or an ester thereof.
[0245] [000245] O) Another embodiment of the invention relates to a compound of formula I shown in claim 1, selected from the group of compounds represented in Table 1 with the following compound numbers: [1], [12], [ 13], [14], [17] and [18], and, in a broader aspect, from the compound [4], a physiologically acceptable salt, and / or an ester thereof.
[0246] [000246] P) An additional embodiment of the invention relates to a preservative or antimicrobial composition, comprising as an active agent a compound or mixture of compounds of formula I, a physiologically acceptable salt, and / or an ester thereof, as shown or defined in any of paragraphs A), C), D) and H) to O), alone or with another additive, such as a carrier material, where the preservative composition is especially for use in a cosmetic, a food, a drink, a pharmaceutical substance, a medical device, or an active packaging material.
[0247] [000247] Q) Another embodiment of the invention relates to the composition according to paragraph P), which is a powder.
[0248] [000248] R) Another embodiment of the invention relates to the composition according to paragraph P), which is a liquid.
[0249] [000249] S) Yet another embodiment of the invention relates to the composition according to paragraph P), which is a coating or film.
[0250] [000250] T) Another embodiment of the invention relates to the composition according to any of the paragraphs P) to S), wherein the preservative or antimicrobial composition is to increase the stability against microorganisms.
[0251] [000251] U) Another embodiment of the invention relates to the composition according to paragraph T), in which the microorganisms are at least one microorganism selected from the group consisting of mold, yeast and bacteria.
[0252] [000252] V) Another embodiment of the invention relates to the composition according to any of paragraphs P) to U), being a preservative or antimicrobial composition for a pharmaceutical substance, a medical device, a food container, a container for drinks or especially a food, drink or cosmetic or household care product.
[0253] [000253] W) Another embodiment of the invention relates to the composition according to any of paragraphs P) to V), which comprises an additional preservative.
[0254] [000254] Y) Another embodiment of the invention relates to the composition according to any of the paragraphs P) to W), which is a precursor to a drink, especially a concentrate, syrup or powder.
[0255] [000255] Z) Another embodiment of the invention relates to an extract comprising one or more compounds of formula I, a physiologically acceptable salt, and / or an ester thereof, as shown or defined in any of paragraphs A), C ), D) or H) to O).
[0256] [000256] AA) Another embodiment of the invention relates to a method of increasing the microbial stability of a material, comprising adding one or more compounds of formula I, a physiologically acceptable salt, and / or an ester thereof, as shown or defined in any of paragraphs A), C), D) or H) to O), to a material, preferably a material selected from the group consisting of a cosmetic, a food, a drink, a pharmaceutical substance, a device doctor, and an active packaging material.
[0257] [000257] BA) Another embodiment of the invention relates to the method of paragraph AA), in which the material is a drink or a food.
[0258] [000258] CA) Another embodiment of the invention relates to the method of paragraph AA), in which the material is a cosmetic.
[0259] [000259] DA) Another embodiment of the invention relates to a material comprising, as or within a coating and / or as a mixture, an additive in the form of a compound or a mixture of compounds of formula I, a physiologically acceptable salt and / or an ester thereof, as defined in any of paragraphs A), C), D) or H) to O).
[0260] [000260] EA) Another embodiment of the invention relates to the material of paragraph DA), which is a cosmetic, a food, a drink, a pharmaceutical substance, a medical device, or an active packaging material.
[0261] [000261] FA) Another embodiment of the invention relates to the material according to paragraph DA), which is a beverage.
[0262] [000262] GA) Another embodiment of the invention relates to the material in the form of a compound or a mixture of compounds of formula I, a physiologically acceptable salt and / or an ester thereof, according to paragraph FA), where the drink is selected from the group consisting of water, flavored water, fortified water, an flavored drink, sparkling water, a juice, Coca-Cola, lemon and lime soda, ginger soda, root beer sodas, which are gaseous in the soft drink style, a syrup, diet drinks, a carbonated soda, fruit juice, other fruit-containing drinks that provide the flavor of fruit juices and contain more than 0% fruit juice, but less than 100% fruit juice fruit juice, fruit flavored drinks, vegetable juices, drinks containing vegetables, which provide the flavor of any of the vegetable juices mentioned above and contain more than 0% vegetable juice, but less than 100% vegetable juice vegetable juice, drinks isotonic, non-isotonic drinks, soft drinks containing fruit juice, coffee, tea, tea drinks prepared from concentrate, extracts, or tea powders, drinkable dairy products, hot chocolate, chocolate powders / mixes, drinkable soy products, milk substitutes, alcoholic drinks, fruit milkshakes, orchata, sports drinks, energy drinks, health drinks, wellness drinks, whipped drinks, protein drinks, drinkable soy yogurts, low acid drinks , acidified drinks, nectars, tonics, frozen carbonated drinks, frozen non-carbonated drinks, liquid meal replacements, infant formulations, and combinations or mixtures thereof.
[0263] [000263] HA) Another embodiment of the invention relates to the material according to paragraph DA), which is a precursor to beverage, especially a concentrate, syrup or powder.
[0264] [000264] IA) Another embodiment of the invention relates to the material according to paragraph DA), which is a food.
[0265] [000265] JA) Another embodiment of the invention relates to the material according to paragraph DA), which is a cosmetic.
[0266] [000266] KA) Another embodiment of the invention relates to the material in the form of a cosmetic according to paragraph JA), which is a skin cream, emulsion, lotion, gel or oil; a facial mask; a colored base; a powder for makeup, a powder after bath, a hygienic powder; a soap, a deodorant soap; a perfume, a toilet water, a cologne, a bath or shower preparation; a depilatory; a deodorant, an antiperspirant, a hair care product; a product; a product to define; a cleaning product; a conditioning product; a hairdresser product; a shaving product; a product to make up and remove makeup from the face and eyes, a product intended for application on the lips, a product for the care of teeth and / or mouth; a product for nail care and painting, a product for intimate external hygiene, a product for sunbathing, a product for sunless tanning, a product for whitening the skin, an anti-wrinkle product, an absorbent tampon, a sanitary napkin, a diaper or a handkerchief.
[0267] [000267] LA) Another embodiment of the invention relates to the cosmetic material according to any of the paragraphs JA) and LA), which comprises one or more additives selected from the group consisting of abrasives, absorbents, pie anti-formation, anti-corrosion, anti-dandruff, antifoam, antimicrobial, antioxidants, antiperspirants, antiplatelet, antiseboreal, antistatic, astringent, binding agents, bleaching agents, buffering agents, bulking agents, chelators, cleaners, cosmetic dyes, denaturants, deodorants, depilators, de-emollients, de-emollients, de-emollients, de-emollients, de-emollients, de-emulsifiers, de-emulsifiers, de-emulsifiers, de-emulsifiers, de-emulsifiers, de-emulsifiers. , emulsion stabilizers, film forming agents, foaming agents, foam reinforcers, gel forming agents, hair conditioners, hair dyes, hair fixers, undulators or hair straighteners, humectants, hydrotropic agents, keratolytics, masking agents, moisturizers , nail conditioners, opacifying agents, oral care, oxidizing agents pearlescent, plasticizers, preservatives, propellants, skin protectors, softeners, solvents, mitigators, stabilizers, surfactants, tanning, tonics, UV absorbers, UV filters, and viscosity controllers.
[0268] [000268] MA) Another embodiment of the invention relates to the cosmetic material according to any of the paragraphs JA) and LA), where the formulations can be or comprise or contain cosmetic additives selected from sunscreens, preservatives, bactericides, fungicides, virucides, refrigerants, insect repellents, plant extracts, anti-inflammatory substances, wound healing accelerators, film-forming substances, usual antioxidants, vitamins, 2-hydroxycarboxylic acids, skin dyes, active ingredients to promote the hair growth, skin care products, softening, moisturizing and / or moisture-maintaining substances, fats, oils, saturated fatty acids, monounsaturated or polyunsaturated fatty acids, alpha hydroxy acids, polyhydroxy fatty acids or their derivatives, alcohols, polyols, polymers, foam stabilizers, electrolytes, organic solvents, silicone derivatives or chelating agents, anti-dandruff substances, hair care products, perfumes, antifoams, dyes, pigments with a coloring action, thickeners, surfactants, emulsifiers, plant parts and plant extracts, animal extracts, proteins, protein hydrolysates, yeast extracts , hops and wheat extracts, peptides and thymus extracts.
[0269] [000269] NA) Another embodiment of the invention relates to the material according to any of the paragraphs DA) to MA) comprising an additional preservative.
[0270] [000270] PA) Another embodiment of the invention relates to the material according to any of the paragraphs DA) to NA), which is obtained after heat treatment, especially at 60 to 130 ° C.
[0271] [000271] QA) Another embodiment of the invention relates to a compound of formula I or a mixture of such compounds, according to any one of paragraphs A), B) and H) to O), or a composition comprising them , especially in accordance with any of the paragraphs P) to S) mentioned above, as a biofilm inhibiting agent and its corresponding use, for example, by administration, or in methods that comprise the administration, of one or more compounds of formula I , or a composition that comprises them, to the surfaces or materials that come into contact with the surfaces.
[0272] (i) bactérias Gram-positivas são selecionadas a partir do grupo que consiste nos gêneros Bacillus, Brevibacterium, Lactobacillus, Micrococcus, Staphylococcus, Streptococcus, Clostridium, Chlamydia, Enterococcus, Listeria, Corynebacterium, Leuconostoc, Pediococcus, Propionibacterium, e os fungos preferivelmente são selecionados a partir do grupo consistindo em (ii-a) fungos das famílias Trichocomaceae, Arthrodermataceae e Mucoraceae, mais preferivelmente fungos (bolores) dos gêneros Aspergillus, Botryotinia, Byssochlamys, Magnaporthe, Paecilomyces, Neosartorya, Mucor, Penicillium, Rhizopus, Talaromyces, e Trichophyton, (ii-b) leveduras da ordem Saccharomycetales, preferivelmente as leveduras das famílias Saccharomycetaceae ou Pichiaceae, mais preferivelmente a partir do grupo que consiste nos gêneros Brettanomyces, Candida, Dekkera, Pichia, Saccharomyces, e Zygosaccharomyces, [000272] In a preferred embodiment, the invention relates to (the use of) one or more compounds of the aforementioned formula I, one or more physiologically acceptable salts of a compound of the aforementioned formula I, or a mixture thereof, as an agent with preservative properties against (i) Gram-positive bacteria and / or (ii) fungi, in which the (i) Gram-positive bacteria are selected from the group consisting of the genera Bacillus, Brevibacterium, Lactobacillus, Micrococcus, Staphylococcus, Streptococcus, Clostridium, Chlamydia, Enterococcus, Listeria, Corynebacterium, Leuconostoc, Pediococcus, and selected from the group consisting of (ii-a) fungi of the families Trichocomaceae, Arthrodermataceae and Mucoraceae, more preferably fungi (molds) of the genera Aspergillus, Botryotinia, Byssochlamys, Magnaporthe, Paecilomyces, Neosartorya, Mucor, Penicillium, Rhizopus, Talichomyces, Talaromyces, e (ii-b) yeasts of the order Saccharomycetales, preferably yeasts of the families Saccharomycetaceae or Pichiaceae, more preferably from the group consisting of the genera Brettanomyces, Candida, Dekkera, Pichia, Saccharomyces, and Zygosaccharomyces,
[0273] [000273] preferably comprising adding the agent to a material, where said material is selected from the group consisting of a cosmetic product, a food product, a beverage, a pharmaceutical product, a medical device, a medical hygiene product, a home care product, and an active packaging material.
[0274] [000274] In a more preferred embodiment, the invention relates to (the use of) one or more compounds of the aforementioned formula I, one or more physiologically acceptable salts of a compound of the aforementioned formula I, or a mixture thereof, where m is 3 to 5, n is 3, o is 0 or 1 and p is 11 to 14, preferably m is 3 to 5, n is 3, o is 0 or 1 and p is 12 or 13, and R is a trisaccharide carbohydrate portion of the sub-formula
[0275] [000275] linked by means of a carbon atom to the bonding oxygen (through the link indicated by the dotted line), where ring A is a portion of xylopyranoside, ring B is a portion of xylopyranosyl, and ring C is a portion of glycopyranosyl, and where one or more of the hydroxyl groups of said rings are esterified with a C2-C10-alkanoic acid, preferably a C3-C10-alkanoic acid, more preferably a C3-C6-alkanoic acid, as an agent with properties preservatives against (i) Gram-positive bacteria and / or (ii) fungi, as indicated above, preferably comprising adding the agent to a material, where said material is preferably selected from the group consisting of a cosmetic product, a food product , a drink, a pharmaceutical product, a medical device, a medical hygiene product, a household care product, and an active packaging material.
[0276] [000276] As already mentioned, and especially due to their excellent and generally superior antimicrobial properties (in particular with respect to yeasts and fungi), the preferred compounds or mixtures of compounds of the formula I shown above are defined by a carbohydrate portion of trisaccharide R that carries at least one hydroxyl group esterified with an acid with 3 or more carbon atoms, particularly where the acid is a C3-C10-alkanoic acid, especially where the acid is a C3-C6-alkanoic acid , and / or a physiologically acceptable salt thereof.
[0277] - bactérias selecionadas a partir do grupo consistindo em Bacillus subtilis, Bacillus cereus, Brevibacterium epidermidis, Brevibacterium linens, Chlamydia trachomatis, Clostridium perfringens, Clostridium botulinum, Clostridium sporogenes, Corynebacterium xerosis, Corynebacterium variabile, Corynebacterium minutissimum, Enterococcus faecalis, Lactobacillus plantarum, Listeria monocytogenes, Listeria welshimeri, Micrococcus luteus, Propionibacterium acnes, Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus mutans, e Streptococcus pneumoniae, e/ou - fungos filamentosos selecionados a partir do grupo consistindo em Aspergillus brasiliensis, Aspergillus flavus, Aspergillus fumigatus, Aspergillus niger, Botrytis cinerea, Byssochlamys fulva, Magnaporthe grisea (Magnaporthe oryzae, Pyricularia oryzae), Mucor plumbeus, Rhizopus arrhizus, Rhizopus nigricans, Rhizopus stolonifer, e Talaromyces luteus, e/ou - levedura selecionada a partir do grupo consistindo em Brettanomyces bruxellensis, Brettanomyces naardenensis, Candida albicans, Candida glabrata, Candida lusitaniae, Candida tropicalis, Dekkera bruxellensis, Dekkera naardenensis, Saccharomyces cerevisiae, Zygosaccharomyces bailii, Zygosaccharomyces bisporus, Zygosaccharomyces florentinus, e Zygosaccharomyces rouxii. [000277] In a particularly preferred embodiment, the invention relates to (the use of) one or more compounds of the aforementioned formula I, one or more physiologically acceptable salts of a compound of the aforementioned formula I, or a mixture of them ( preferably as defined in one of the preferred or particularly preferred embodiments herein) as an agent with preservative properties against - Bacteria selected from the group consisting of Bacillus subtilis, Bacillus cereus, Brevibacterium epidermidis, Brevibacterium linens, Chlamydia trachomatis, Clostridium perfringens, Clostridium botulinum, Clostridium sporogenes, Corynebacterium xerosis, Corynebacterium variate, Cysnachernum , Listeria welshimeri, Micrococcus luteus, Propionibacterium acnes, Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus mutans, and Streptococcus pneumoniae, and / or - filamentous fungi selected from the group consisting of Aspergillus brasiliensis, Aspergillus flavus, Aspergillus fumigatus, Aspergillus niger, Botrytis cinerea, Byssochlamys fulva, Magnaporthe grisea (Magnaporthe oryzae, Pyricularia oryize, Rhyorous, Rhino, and Talaromyces luteus, and / or - yeast selected from the group consisting of Brettanomyces bruxellensis, Brettanomyces naardenensis, Candida albicans, Candida glabrata, Candida lusitaniae, Candida tropicalis, Dekkera bruxellensis, Dekkera naardenensis, Saccharomyces cerevisiae, Zygosaccharomycesychaacyromyacisacchi, Zygosaccharomgoscesaccha, Zygosaccharomgoscesaccha, Zygosaccharomgoscesaccha, Zygosaccharomgoscesaccha, Zygosaccharomgoscesacchi, Zygosaccharomycesyac,
[0278] [000278] Within the scope of this text, the terms "for oral consumption", "orally consumable" or "food product" and the like, in particular, refer to materials that are intended to be swallowed by a human in a unchanged state (ie, by direct oral consumption, "ready to eat", "ready to drink") or processed and then digested.
[0279] [000279] The term "ready to use" product refers to a product whose composition, in terms of the substances that determine the taste, is (essentially) complete. The term "ready to use" product includes gaseous and non-gaseous liquids and viscous or semi-solid products. Examples of "ready to use" products include frozen products that, before consumption, are to be defrosted and heated before consumption. Ready-to-use products may also be "ready to eat" or "ready to drink", such as carbonated drinks, flavored milk, ice (water), yogurt, and the like, or may have to be diluted with water before oral consumption, which is, for example, the case for beverage syrups.
[0280] [000280] Yeasts are able to develop in orally consumable compositions, such as food and beverages, with low pH values (usually pH 5.0 or less), and in the presence of sugars, organic acids or carbon sources easily metabolized. During their development, yeasts metabolize some components of food and produce metabolic products. This causes the physical, chemical, and sensory properties of an orally consumable composition to change, and the composition is deteriorated. The development of yeast within orally consumable compositions is often seen on its surface, as in cheeses or meats, or by the fermentation of sugars in drinks, such as juices, and in semi-liquid products, such as syrups and jams.
[0281] [000281] Of particular relevance in the context of orally consumable compositions are Aspergillus niger, Brettanomyces bruxellensis, Brettanomyces naardenensis, Dekkera bruxellensis, Dekkera naardenensis, Saccharomyces cerevisiae, Zygosaccharomyces bailii, Zygosacusinchuschususususychischusacus, Zygosacusinus the Zygosaccharomyces rouxii.
[0282] [000282] Yeasts of the genus Zygosaccharomyces have had a long history as deteriorating yeasts within the food industry. This is mainly due to the fact that these species can develop in the presence of high concentrations of sucrose, ethanol, acetic acid, sorbic acid, benzoic acid, and sulfur dioxide (which are some of the preservatives commonly used in orally consumable compositions).
[0283] [000283] Clostridium botulinum is a Gram-positive bacterium that produces several toxins, among others, neurotoxins that cause the flaccid muscle paralysis seen in botulism. Botulism poisoning can occur due to improperly preserved food or canned food that has not been processed using the correct preservation times and / or pressure. Especially slightly acidic or neutral food is at risk, which has been stored under anaerobic conditions (usually pH> 4.6) and storage temperatures above 10 ° C. This is generally applied to canned foods, such as canned meat and fish, mayonnaise, but also slightly acidic fruit or vegetables.
[0284] [000284] Bacillus cereus can be harmful to humans and cause foodborne illnesses (severe nausea, vomiting and diarrhea), particularly in foods such as meat, milk, condiments, spices, fruits, vegetables, cereal and cereal products, rice products and portions of rice (ready to eat). Bacillus cereus is also known to cause chronic skin infections and keratitis.
[0285] [000285] Bacillus subtilis is known to cause disease in severely immunocompromised patients, and it can cause food poisoning. Bacillus subtilis spores can survive extreme heat during cooking. Strains of Bacillus subtilis are responsible for causing viscosity in the damaged bread dough.
[0286] [000286] Micrococcus luteus is found in soil, dust, water and air, and is part of the normal flora of the skin and mucous membranes of mammals. It is also a bacterium that spoils food and is often found on spoiled meat. In immunocompromised patients, Micrococcus luteus can cause infections.
[0287] [000287] Propionibacterium acnes is quite commensal and is part of the flora of human skin in a healthy adult. It lives mainly on the fatty acids in the sebum secreted by sebaceous glands in the follicles and is linked to the acne skin condition. Propionibacterium acnes can also cause chronic blepharitis and endophthalmitis, particularly after intraocular surgery.
[0288] [000288] Dental plaque is a biofilm formed by colonization of bacteria that try to stick to a smooth tooth surface. A microorganism that significantly contributes to dental plaque and caries is Streptococcus mutans.
[0289] [000289] The rice rust fungus Magnaporthe grisea (syn .: Pyricularia oryzae; conidia / anamorph stage: Pyricularia grisea) attacks leaves, grains, and other parts of rice plants.
[0290] [000290] Athlete's foot (tinea pedis) is a contagious disease caused by parasitic fungi in the genus Trichophyton, predominantly Trichophyton rubrum and / or Trichophyton mentagrophytes. These can also cause skin infections over other areas of the body, most often under the toe nails (onychomycosis) or over the groin (crural ringworm).
[0291] [000291] Mucor species are often involved in fertilization with plant compost and plant residues and are found on foods, such as milk, butter, cheese and tomatoes. Mucor plumbeus has a worldwide distribution in the soil. As a deteriorating germ, Mucor plumbeus is mainly found on fermented foods (such as bread, beer, wine, cheese, yogurt, sour milk, salami), and on grain.
[0292] [000292] Mucormycosis (sometimes also referred to as Zygomycosis) is the term used to describe fungal infections caused by fungi in the order Mucorales, among others, by species in the genus Mucor. These rare but serious and potentially fatal fungal infections usually affect the face, the oropharyngeal cavity (nose / mouth), the gastrointestinal tract or the skin. Individuals with immune disorders (immunocompromised) are more prone to this type of fungal infection.
[0293] [000293] Rhizopus is a genus of fungi found on plants and on several other organic substrates, including ripe fruits and vegetables, jellies, syrups, bread, peanuts and tobacco. Some Rhizopus species are opportunistic agents of human zygomycosis (fungal infection) and can be fatal. Rhizopus infections are also an associated complication of diabetic ketoacidosis.
[0294] [000294] Rhizopus arrhizus is the most common cause of mucormycosis in humans and occasionally infects other animals.
[0295] [000295] Rhizopus nigricans is a fungus commonly known as bread mold and is the most common species of Rhizopus. It is found on the old food. Spores, spread in hot dry weather, contain allergenic proteins, which can produce respiratory and nasal symptoms. Food workers are particularly at risk if they are allergic to mold.
[0296] [000296] Rhizopus stolonifer (black bread mold) is a widely distributed mold and is most commonly found on bread and soft fruits, such as bananas and grapes, and causes damage to the surface where it lives. It is capable of causing opportunistic infections in humans.
[0297] [000297] Staphylococcus aureus is the most common species to cause staphylococcal infections. Staphylococcus can cause a variety of diseases, from skin infections to life-threatening diseases, such as pneumonia, meningitis, osteomyelitis, endocarditis, toxic shock syndrome, bacteremia, and sepsis. Strains of Staphylococcus aureus are also responsible for poisoning food through the production of an enterotoxin, particularly in meat, meat products (eg, bologna, hams, sausages), milk, milk products, such as cheese .
[0298] [000298] Clostridium perfringens is widely present in nature and can be found as a normal component of decomposing vegetation, but also in the intestinal tract of humans. Clostridium perfringens bacteria are often the cause of foodborne illness, particularly in meat and poultry poorly prepared. Often, the meat is well prepared, but too early before consumption. Since Clostridium perfringens forms spores that can withstand cooking temperatures, under rest or storage germination occurs and infectious bacterial colonies develop. Clostridium perfringens causes a wide variety of symptoms: it is a very common cause of food poisoning and the most common bacterial agent for gas gangrene, which is necrosis, tissue rot, and gas production.
[0299] [000299] Fungi of the Aspergillus genus can cause infections that cause a variety of diseases called aspergillosis (the common forms are allergic bronchopulmonary aspergillosis, pulmonary aspergilloma and invasive aspergillosis).
[0300] [000300] Aspergillus flavus is a common mold in the environment, and can cause storage problems in stored grains. It can also be a human pathogen, associated with aspergillosis and other infections.
[0301] [000301] Aspergillus fumigatus is one of the most common Aspergillus species to cause disease in individuals with an immunodeficiency. In immunocompromised individuals, such a patient receiving immunosuppressive therapy for autoimmune or neoplastic disease, the organ transplant recipient, and people with AIDS or leukemia, the fungus is more likely to become pathogenic and cause aspergillosis. Aspergillus fumigatus mainly causes invasive lung infection (for example, chronic lung infections) and represents a major cause of morbidity and mortality in these individuals.
[0302] [000302] Aspergillus niger causes black mold on certain fruits and vegetables, such as grapes, onions, and peanuts, and is a common food contaminant. For example, Aspergillus niger causes a common disease after harvesting onions. Aspergillus niger is less likely to cause human disease than some other species of Aspergillus, however, if large amounts of spores are inhaled, serious lung disease (aspergillosis) can occur. Aspergillus niger is one of the most common causes of otomycosis (fungal infections of the ear).
[0303] [000303] Chlamydia infection is one of the most common sexually transmitted infections in humans, and is caused by the bacterium Chlamydia trachomatis. Chlamydia is a major infectious cause of human genital and eye disease. Conjunctivitis or chlamydia trachoma is a common cause of blindness worldwide. Both sexes can show urethritis, proctitis, trachoma, and infertility. If left untreated, chlamydia infections can cause serious health problems. Chlamydia trachomatis is also an important neonatal pathogen, where it can lead to infections of the eye (trachoma) and pulmonary complications.
[0304] [000304] Enterococcus faecalis inhabits the gastrointestinal tracts of humans and other mammals. It can cause endocarditis and bacteremia, urinary tract infections, meningitis, and other infections in humans (for example, teeth with treated dental canals). It can even cause potentially fatal infections in humans, especially in the hospital environment.
[0305] [000305] Listeria can be found in uncooked meat, uncooked vegetables, fruit, pasteurized or unpasteurized milk, foods prepared from milk, and processed foods. Pasteurization and sufficient cooking kill Listeria; however, contamination can occur after cooking and before packaging. For example, processing plants that produce ready-to-eat foods, such as hot dogs, sausages, fish products, cheese, milk, and spice salads, follow extensive sanitation policies and procedures to prevent contamination by Listeria. The main human pathogen in the genus Listeria is Listeria monocytogenes. It is usually the causative agent of listeriosis, a serious bacterial infection, caused by eating food contaminated with Listeria monocytogenes.
[0306] [000306] Yeasts of the genus Candida are a group of opportunistic pathogens that cause oral and vaginal infections in humans, known as candidiasis. The pathogenic yeasts of candidiasis are Candida albicans, Candida tropicalis, Candida stellatoidea, Candida glabrata, Candida krusei, Candida parapsilosis, Candida guilliermondii, Candida viswanathii, and Candida lusitaniae, of these, Candida albicans being the most important and the most relevant.
[0307] [000307] Candida glabrata is the second most common Candida pathogen after Candida albicans, also causing infections of the urogenital tract and bloodstream (candidemia). Candida glabrata has been shown to be a highly opportunistic pathogen and is especially prevalent in immunocompromised individuals and the elderly. Candida glabrata can also adhere to biotic and abiotic surfaces, thereby forming microbial "biofilms" over, for example, urinary probes or intravenous delay probes. It can also cause problems with dental devices, such as dentures.
[0308] [000308] In addition, in particular Staphylococcus aureus, Candida albicans, Aspergillus brasiliensis and Aspergillus niger are probably microbiological contaminants in cosmetic formulations.
[0309] [000309] Species of certain bacteria, such as Staphylococcus epidermidis, Corynebacterium xerosis, Corynebacterium minutissimum and Brevibacterium epidermidis, are largely responsible for the formation of odor in the armpit and / or feet, or for body odor in general. Brevibacterium linens inter alia causes odor in the feet.
[0310] [000310] In another aspect, the present invention relates to one or more compounds of formula I and / or their physiologically acceptable salts, particularly one or more compounds selected from the group consisting of [1], [2], [3], [4], [5], [6], [7], [8], [9], [10], [11], [12], [13], [14], [18 ], [19], [20], [21] and their physiologically acceptable salts, for use in the prophylactic and / or therapeutic treatment of a disorder, disease or condition selected from the group consisting of mycoses (fungal infections), preferably mycoses associated with Aspergillus, Candida or Mucor, particularly mycoses associated with Candida.
[0311] [000311] Particularly relevant and preferably treated mycoses are selected from the group consisting of candidiasis [in particular oral Candida infections (cold sores), urogenital tract infections (eg vaginal) by Candida bacteria (in particular Candida albicans and / or Candida glabrata), diaper candidiasis (diaper rash associated with Candida, diaper rash)], invasive candidiasis (particularly candidemia (bloodstream infections)), aspergillosis and mucormycosis.
[0312] [000312] The compounds of formula I and / or the physiologically acceptable salts thereof, particularly those selected from the group consisting of [1], [2], [3], [4], [5], [6] , [7], [8], [9], [10], [11], [12], [13], [14], [18], [19], [20], [21] and the physiologically acceptable salts thereof, are particularly beneficial for use in the prophylactic treatment of a disorder, disease or condition mentioned above.
[0313] [000313] As used herein, the "effective amount" or "effective dose" refers to the administration (preferably oral) of an effective dose of one or more compounds of formula I and / or the physiologically acceptable salts thereof that it produces the effects for which it is administered.
[0314] [000314] As used herein, the term "therapeutic" or "therapeutically" refers to the administration (in particular, oral) of a therapeutically effective dose of one or more compounds of formula I and / or their physiologically acceptable salts ( preferably in the form of a mixture, composition or material as defined in the context of the present invention) which produces the effects for which it is administered, that is, which will elicit the biological or medical response (in vitro or in vivo, preferably in live in a mammal, particularly in vivo in humans) that is being sought, in particular to improve and / or alleviate the symptoms of the disorder, disease or condition being treated until, and including, complete cure.
[0315] [000315] As used herein, the term "prophylactic" or "prophylactically" refers to the administration (in particular, oral) of a prophylactically effective dose of one or more compounds of formula I (preferably in the form of a mixture, a composition or material as defined in the context of the present invention) that produces the effects for which it is administered, that is, that will elicit the biological or medical response (in vitro or in vivo, preferably in vivo in a mammal, particularly in vivo in a human being) that is being sought, in particular the prevention of the onset of a disorder, disease or condition in individuals who are at risk of such disorder, disease or condition as mentioned in this document.
[0316] - um ou mais compostos de fórmula I como definidos neste documento, e/ou um ou mais sais fisiologicamente aceitáveis deles, particularmente um ou mais compostos selecionados a partir do grupo consistindo em [1], [2], [3], [4], [5], [6], [7], [8], [9], [10], [11], [12], [13], [14], [18], [19], [20], [21] e os sais fisiologicamente aceitáveis do mesmo,ou - um extrato ou material de acordo com a presente invenção, preferivelmente em uma modalidade preferida ou particularmente preferida de acordo com a presente invenção. [000316] The present invention also relates to a method of reducing the activity and / or the number of pathogenic Gram-positive bacteria and / or pathogenic fungi in an immunocompromised individual, comprising the following step: administer (preferably, orally or topically) to a mammal, particularly an immunocompromised mammal, particularly an immunosuppressed human, a total amount of - one or more compounds of formula I as defined herein, and / or one or more physiologically acceptable salts thereof, particularly one or more compounds selected from the group consisting of [1], [2], [3], [4 ], [5], [6], [7], [8], [9], [10], [11], [12], [13], [14], [18], [19], [20], [21] and the physiologically acceptable salts thereof, or - an extract or material according to the present invention, preferably in a preferred or particularly preferred embodiment according to the present invention.
[0317] [000317] Preferably, the pathogenic Gram-positive bacteria are selected from the group consisting of the genera Bacillus, Clostridium, Listeria, Micrococcus and Staphylococcus, more preferably selected from the group consisting of Bacillus cereus, Clostridium sporogenes, Clostridium perfringens, Listeria monocytogenes, Micrococcus luteus, Staphylococcus aureus, Staphylococcus epidermidis.
[0318] [000318] Preferably, pathogenic fungi are selected from the group consisting of the genera Aspergillus and Candida, most preferably selected from the group consisting of Aspergillus flavus, Aspergillus fumigatus, Candida albicans, Candida glabrata, Candida lusitaniae, and Candida tropicalis .
[0319] - um ou mais compostos de fórmula I como definidos neste documento, e/ou um ou mais sais fisiologicamente aceitáveis deles, particularmente um ou mais compostos selecionados a partir do grupo consistindo em [1], [2], [3], [4], [5], [6], [7], [8], [9], [10], [11], [12], [13], [14], [18], [19], [20], [21], e os sais fisiologicamente aceitáveis do mesmo,ou - um extrato ou material de acordo com a presente invenção, preferivelmente em uma modalidade preferida ou particularmente preferida de acordo com a presente invenção. [000319] The present invention also relates to a method for the prophylactic and / or therapeutic treatment of a disease, disorder or condition, comprising the following step: administer (preferably, orally or topically) to a mammal, particularly an immunocompromised mammal, particularly an immunosuppressed human, a total effective amount of - one or more compounds of formula I as defined herein, and / or one or more physiologically acceptable salts thereof, particularly one or more compounds selected from the group consisting of [1], [2], [3], [4 ], [5], [6], [7], [8], [9], [10], [11], [12], [13], [14], [18], [19], [20], [21], and the physiologically acceptable salts thereof, or - an extract or material according to the present invention, preferably in a preferred or particularly preferred embodiment according to the present invention.
[0320] [000320] The compounds of formula I (for use) according to the present invention and / or their physiologically acceptable salts (preferably, the preferred or particularly preferred compounds of formula I, defined above) show comparatively weak activity against Gram-negative bacteria, such as Escherichia coli, Pseudomonas aeruginosa, Pseudomonas putida or Salmonella typhimurium. In our own investigations regarding Gram-negative bacteria, MIC values> 100 ppm were generally observed, typically in the range of 200 - 500 ppm.
[0321] [000321] Thus, in a preferred embodiment, a material according to the present invention, preferably a cosmetic or a pharmaceutical substance, comprises (i) one or more compounds of formula I (for use) according to the present invention and / or the physiologically acceptable salts thereof, and (ii) one or more agents that exhibit an antimicrobial activity against Gram-negative bacteria, preferably selected from the group consisting of triclosan® (2,4,4'-trichloroether) 2'-hydroxydiphenyl), chlorhexidine, chlorhexidine salts (preferably chlorhexidine diacetate, chlorhexidine dichloride, chlorhexidine digliconate), octenidine, octenidine dihydrochloride, 2-bromo-2-nitropropane-1,3-diylamine, polyamine urea, diazolidinyl urea, chlorphenenesin, DMDM hydantoin, sodium hydroxymethylglycinate, phenoxyethanol, isothiazolinones (preferably methyl isothiazolinone, methyl chloro isothiazolinone), benzalkonium chloride (alkyldim chloride ethylbenzylammonium, preferably N-octyl-N-benzyl-N, N-dimethylammonium chloride, N-decyl-N-benzyl-N, N-dimethylammonium chloride, N-dodecyl-N-benzyl-N, N-dimethylammonium chloride , N-tridecyl-N-benzyl-N, N-dimethylammonium chloride, N-tetradecyl-N-benzyl-N, N-dimethylammonium chloride, N-hexadecyl-N-benzyl-N, N-dimethylammonium chloride of N-octadecyl-N-benzyl-N, N-dimethylammonium), and lantibiotics (preferably those disclosed in US 7,960,505 B2).
[0322] [000322] In some cases, a material according to the present invention, preferably a cosmetic or a pharmaceutical substance, comprises (i) one or more compounds of formula I (for use) according to the present invention and / or the salts physiologically acceptable substances, and (ii) one or more parabens (parahydroxybenzoic acid esters) and / or the salts thereof, preferably one, two or more parabens selected from the group consisting of methylparaben, ethylparaben, propylparaben, isopropylparaben , butylparaben, isobutylparaben, benzylparaben and their physiologically acceptable salts (preferably the sodium salts).
[0323] [000323] The compounds of formula I, mixtures and physiologically acceptable salts thereof are preferred in the context of the present invention, where R is a portion of formula
[0324] [000324] The compounds of formula I, their mixtures and the physiologically acceptable salts thereof are more preferred in the context of the present invention, where R is a portion of formula
[0325] [000325] Individual compounds of formula I with an acyl substituent with more than 2 carbon atoms, such as an isovaleryl substituent, in the carbohydrate portion of trisaccharide R exhibit a stronger antimicrobial activity, particularly against yeasts and fungi, especially against yeasts and fungi of relevance in relation to the deterioration of food, beverages and / or cosmetics, and / or a broader spectrum of activity than the corresponding compounds with an acetyl substituent in the R trisaccharide carbohydrate portion.
[0326] [000326] It was also found in the investigations themselves that mixtures comprising (i) two, three, four, five, six or more compounds of the aforementioned preferred formula I, (ii) two, three, four, five, six or more salts physiologically acceptable compounds of a compound of the aforementioned preferred formula I, or a mixture thereof, or (iii) one, two, three or more compounds of the aforementioned preferred formula I and one, two, three or more physiologically acceptable salts of a compound of the the preferred formula I mentioned above typically showed a broader spectrum of activity and / or a stronger long-term inhibitory activity, particularly against yeasts and fungi, especially against yeasts and fungi of relevance to the deterioration of food, beverages and / or cosmetics, compared to the individual compounds of formula I (preferred) or a physiologically acceptable salt thereof.
[0327] [000327] Said stronger long-term inhibitory activity - which is typically bactericidal and / or fungicidal, and not merely bacteriostatic and / or fungistatic - and / or the broader spectrum of long-lasting inhibitory activity of mixtures according to The present invention against organisms involved in the deterioration of preparations or compositions with a high water content (generally 50% by weight or more, based on the total weight of the preparation or composition) is especially useful against acidophilic yeasts of deterioration, which are therefore example, involved in the spoilage of drinks These mixtures according to the present invention are particularly capable of inhibiting the growth of thermophilic fungi, which are difficult to control with standard sterilization and / or pasteurization processes.
[0328] (a) um ou mais compostos de fórmula I ou um sal fisiologicamente aceitável dos mesmos, em que um grupo de Rx, Ry, e Rz não é hidrogênio, (b) um ou mais compostos de fórmula I ou um sal fisiologicamente aceitável dos mesmos, em que dois grupos de Rx, Ry, e Rz não são hidrogênio, e (c) um ou mais compostos de fórmula I ou um sal fisiologicamente aceitável dos mesmos, em que três grupos de Rx, Ry, e Rz não são hidrogênio, em que preferivelmente- a quantidade total dos compostos do grupo (a) é 2% em peso ou mais, preferivelmente 3% em peso ou mais, mais preferivelmente 5% em peso ou mais, em cada caso com base no peso total da misturae/ou- a dita mistura compreende um, dois, três ou todos os compostos [5], [8], [12], e [14],e/ou- a quantidade total dos compostos do grupo (b) é 20% em peso ou mais, preferivelmente 25% em peso ou mais, mais preferivelmente 30% em peso ou mais, ainda mais preferivelmente 35% em peso ou mais, mais preferivelmente 40% em peso ou mais, em cada caso com base no peso total da misturae/ou- a dita mistura compreende um, dois, três, quatro, cinco ou todos os compostos [1], [6], [7], [10], [13], e [18], mais preferivelmente compreende [1] e/ou [7], em particular, mais preferivelmente [1] e [7],e/ou- a dita mistura compreende o composto [1], preferivelmente em uma quantidade total de 1% em peso ou mais, mais preferivelmente 2% em peso ou mais, ainda mais preferivelmente 4% em peso ou mais, em particular, preferivelmente 5% em peso ou mais,e/ou- a dita mistura compreende o composto [7], preferivelmente em uma quantidade total de 5% em peso ou mais, mais preferivelmente 8% em peso ou mais, ainda mais preferivelmente 10% em peso ou mais, em particular, preferivelmente 12% em peso ou mais,e/ou- a quantidade total dos compostos do grupo (c) é 1% em peso ou mais, preferivelmente 1,5% em peso ou mais, mais preferivelmente 2% em peso ou mais, em cada caso com base no peso total da misturae/ou- a dita mistura compreende [3] e/ou [9], preferivelmente o composto [9].[000328] It is especially preferred in the context of the present invention, in view of the excellent properties, a mixture of three or more compounds of formula I or of the physiologically acceptable salts thereof, said mixture comprising (a) one or more compounds of formula I or a physiologically acceptable salt thereof, where a group of Rx, Ry, and Rz is not hydrogen, (b) one or more compounds of formula I or a physiologically acceptable salt thereof, wherein two groups of Rx, Ry, and Rz are not hydrogen, and (c) one or more compounds of formula I or a physiologically acceptable salt thereof, where three groups of Rx, Ry, and Rz are not hydrogen, where preferably - the total amount of the compounds in group (a) is 2% by weight or more, preferably 3% by weight or more, more preferably 5% by weight or more, in each case based on the total weight of the mixture and / or - said mixture comprises one, two, three or all compounds [5], [8], [12], and [14], and / or - the total amount of the compounds of group (b) is 20% by weight or more, preferably 25% by weight or more, more preferably 30% by weight or more, even more preferably 35% by weight or more, more preferably 40% by weight or more, in each case based on the total weight of the mixture and / or - said mixture comprises one, two, three, four, five or all compounds [1], [6], [7], [10], [13], and [18], more preferably comprises [1] and / or [7], in particular, more preferably [1] and [7], and / or - said mixture comprises the compound [1], preferably in a total amount of 1% by weight or more, more preferably 2% by weight or more, even more preferably 4% by weight or more, in particular, preferably 5% in weight or more, and / or - said mixture comprises the compound [7], preferably in a total amount of 5% by weight or more, more preferably 8% by weight or more, even more preferably 10% by weight or more, in particular, preferably 12% by weight weight or more, and / or - the total amount of the compounds of group (c) is 1% by weight or more, preferably 1.5% by weight or more, more preferably 2% by weight or more, in each case based on the total weight of the mixture and / or - said mixture comprises [3] and / or [9], preferably the compound [9].
[0329] [000329] In a preferred mixture according to the present invention, preferably the average degree of acylation is as follows: 1.1 to 2.2 acetyl groups per molecule, preferably 1.3 to 1.9 acetyl groups per molecule, and / or 0.1 to 1.0 isovaleryl groups per molecule, preferably 0.15 to 0.6 isovaleryl groups per molecule,
[0330] [000330] wherein the average degree of acylation is preferably determined by quantification by 1 H-NMR using an average molecular weight for glycolipids of 985 g / mol. As an internal standard, 1,3,5-trichlorbenzene can preferably be used. The 1H NMR signal used for said quantification was that of the hydrogen atom attached to the carbon atom at the C-2 position (ie (CH) OH, the carbon atom containing the alpha-hydroxy group in relation to the group carboxylic acid at C-1).
[0331] [000331] A mixture according to the present invention, an extract according to the present invention, a material according to the present invention, and / or a composition according to the present invention preferably comprises less than 25% by weight Glycine IVA ((2S, 16R, 17S, 21R) -2,16,17-trihydroxy-21 - [[2-O- [2-O- (6-O-acetyl-β-D-glycopyranosyl ) β-D-xylopyranosyl] -4-O-acetyl-β-D-xylopyranosyl] oxy] hexacosanoic), more preferably less than 20% by weight of Glycine IVA, particularly and preferably less than 15% by weight of Glycine VAT in each case based on the total amount of compounds of formula I and their physiologically acceptable salts.
[0332] [000332] Preferably, a mixture comprises compounds [1] and [7] and their physiologically acceptable salts, where more preferably the amount of
[0333] [000333] compound [1] and the physiologically acceptable salts thereof is 1% by weight or more, more preferably 2% by weight or more, more preferably 3% by weight or more, and / or
[0334] [000334] compound [7] and the physiologically acceptable salts thereof is 1% by weight or more, more preferably 2% by weight or more, more preferably 3% by weight or more, and / or
[0335] [000335] of the compounds [1] and [7], and the physiologically acceptable salts thereof, is 5% by weight or more, more preferably 8% by weight or more, even more preferably 12% by weight or more,
[0336] [000336] the weight percentages in each case referring to the total weight of the mixture.
[0337] [000337] More preferably, a mixture according to the present invention comprises
[0338] [000338] a total amount of 1 - 20% by weight, preferably 2 - 15% by weight, of compound [1] and the physiologically acceptable salts thereof,
[0339] [000339] a total amount of 0 - 10% by weight, preferably 0.5 - 5% by weight, of compound [5] and the physiologically acceptable salts thereof,
[0340] [000340] a total amount of 0 - 10% by weight, preferably 0.5 - 5% by weight, of compound [6] and the physiologically acceptable salts thereof,
[0341] [000341] a total amount of 2 - 75% by weight, preferably 5 - 50% by weight, of compound [7] and the physiologically acceptable salts thereof,
[0342] [000342] a total amount of 0 - 12% by weight, preferably 1 - 8% by weight, of compound [8] and the physiologically acceptable salts thereof,
[0343] [000343] a total amount of 0 - 12% by weight, preferably 1 - 8% by weight, of compound [9] and the physiologically acceptable salts thereof,
[0344] [000344] a total amount of 0 - 12% by weight, preferably 1 - 10% by weight, of compound [10] and the physiologically acceptable salts thereof,
[0345] [000345] a total amount of 0 - 10% by weight, preferably 0.5 - 6% by weight, of compound [12] and the physiologically acceptable salts thereof,
[0346] [000346] a total amount of 0 - 8% by weight, preferably 0.25 - 4% by weight, of compound [13] and the physiologically acceptable salts thereof,
[0347] [000347] a total amount of 0 - 8% by weight, preferably 0.25 - 5% by weight, of compound [18] and the physiologically acceptable salts thereof,
[0348] - a dita mistura compreende uma quantidade total de compostos de fórmula I e os sais fisiologicamente aceitáveis dos mesmos de 75% em peso ou mais, preferivelmente 80% em peso ou mais, mais preferivelmente 85% em peso ou mais, - a dita mistura compreende menos do que 8% em peso, preferivelmente menos do que 6% em peso, mais preferivelmente menos do que 4% em peso, de compostos de fórmula I sem nenhum substituinte de acila na porção de carboidrato de trissacarídeo R (particularmente menos do que 2,0% em peso de composto [16]), - a dita mistura compreende Glicenina IVA em uma quantidade de 25% em peso ou menos, preferivelmente de 20% em peso ou menos, mais preferivelmente de 15% em peso ou menos, - a dita mistura compreende proteínas em uma quantidade total de 2% em peso ou menos, preferivelmente de 1,25% em peso ou menos, mais preferivelmente de 1,0% em peso ou menos,e/ou - a dita mistura compreende água em uma quantidade total de 4% em peso ou menos, preferivelmente de 3% em peso ou menos, mais preferivelmente de 2% em peso ou menos, [000348] and additional and preferably one, several or all of the following additional parameters apply: - said mixture comprises a total amount of compounds of formula I and the physiologically acceptable salts thereof of 75% by weight or more, preferably 80% by weight or more, more preferably 85% by weight or more, - said mixture comprises less than 8% by weight, preferably less than 6% by weight, more preferably less than 4% by weight, of compounds of formula I without any acyl substituent in the carbohydrate portion of trisaccharide R ( particularly less than 2.0% by weight of compound [16]), - said mixture comprises Glycine IVA in an amount of 25% by weight or less, preferably 20% by weight or less, more preferably 15% by weight or less, - said mixture comprises proteins in a total amount of 2% by weight or less, preferably 1.25% by weight or less, more preferably 1.0% by weight or less, and / or - said mixture comprises water in a total amount of 4% by weight or less, preferably 3% by weight or less, more preferably 2% by weight or less,
[0349] [000349] the percentages in each case referring to the total weight of the mixture.
[0350] [000350] Particularly and preferably, a mixture according to the present invention comprises
[0351] [000351] a total amount of 3 - 15% by weight of compound [1] and the physiologically acceptable salts thereof,
[0352] [000352] a total amount of 0.5 - 5% by weight of compound [5] and the physiologically acceptable salts thereof,
[0353] [000353] a total amount of 0.5 - 5% by weight of compound [6] and the physiologically acceptable salts thereof,
[0354] [000354] a total amount of 10 - 35% by weight of compound [7] and the physiologically acceptable salts thereof,
[0355] [000355] a total amount of 1 - 8% by weight of compound [8] and the physiologically acceptable salts thereof,
[0356] [000356] a total amount of 1 - 8% by weight of compound [9] and the physiologically acceptable salts thereof,
[0357] [000357] a total amount of 1 - 10% by weight of compound [10] and the physiologically acceptable salts thereof,
[0358] [000358] a total amount of 0.5 - 6% by weight of compound [12] and the physiologically acceptable salts thereof,
[0359] [000359] a total amount of 0.25 - 4% by weight of compound [13] and the physiologically acceptable salts thereof, and
[0360] [000360] a total amount of 0.25 - 5% by weight of compound [18] and the physiologically acceptable salts thereof,
[0361] - a dita mistura compreende uma quantidade total de compostos de fórmula I e os sais fisiologicamente aceitáveis dos mesmos de 85% em peso ou mais, mais preferivelmente de 90% em peso ou mais, - a dita mistura compreende menos do que 5% em peso, preferivelmente menos do que 3% em peso, de compostos de fórmula I sem nenhum substituinte de acila na porção de carboidrato de trissacarídeo R (particularmente menos do que 1,0% em peso de composto [16]), - a dita mistura compreende Glicenina IVA em uma quantidade de 20% em peso ou menos, preferivelmente de 15% em peso ou menos, - a dita mistura compreende proteínas em uma quantidade total de 1,25% em peso ou menos, preferivelmente de 0,95% em peso ou menos.e/ou - a dita mistura compreende água em uma quantidade total de 3% em peso ou menos, preferivelmente de 2% em peso ou menos, as porcentagens em cada caso referindo-se ao peso total da mistura. [000361] and additional and preferably one, two, three, four or all of the following additional parameters apply: - said mixture comprises a total amount of compounds of formula I and the physiologically acceptable salts thereof of 85% by weight or more, more preferably 90% by weight or more, - said mixture comprises less than 5% by weight, preferably less than 3% by weight, of compounds of formula I without any acyl substituent in the carbohydrate portion of trisaccharide R (particularly less than 1.0% by weight) of compound [16]), - said mixture comprises Glycine IVA in an amount of 20% by weight or less, preferably 15% by weight or less, - said mixture comprises proteins in a total amount of 1.25% by weight or less, preferably 0.95% by weight or less. and / or - said mixture comprises water in a total amount of 3% by weight or less, preferably 2% by weight or less, the percentages in each case referring to the total weight of the mixture.
[0362] [000362] Such a particularly preferred mixture according to the present invention had the following average degree of acylation; 1.4 to 1.8 acetyl groups per molecule, and 0.2 to 0.5 isovaleryl groups per molecule.
[0363] [000363] In view of their broad spectrum of activity and particularly high efficacy (which has been found to be superior to otherwise identical compounds of formula I that do not carry a hydroxyl group esterified with isovaleric acid), particularly against yeasts and fungi , especially against yeasts and fungi of relevance to the deterioration of food, beverages and / or cosmetics, compounds of formula I, mixtures and physiologically acceptable salts thereof are especially preferred in the context of the present invention, where m is 3, 4 or 5, n is 3, o is 0 or 1 and p is 11 to 14 (preferably p is 12 or 13), and R is a portion of formula
[0364] [000364] Particularly preferred are compounds of formula I of the following formulas
[0365] [000365] Particularly preferred are the compounds of formulas [1a], [12a], [13a], [18a], [19a], [20a], and [21a], mixtures and physiologically acceptable salts thereof [1a]
[0366] [000366] Particularly preferred are the following compounds of formulas [1b], [12b], [13b] and [18b], mixtures and physiologically acceptable salts thereof
[0367] [000367] Particularly preferred are the following compounds of formulas [1c], [12c], [13c] and [18c], mixtures and physiologically acceptable salts thereof
[0368] [000368] In a preferred embodiment, the present invention relates to a composition that comprises or consists of three, four, five, six, seven, eight, nine, ten, eleven, twelve or more compounds of formula I, wherein the total amount of said compounds of formula I is greater than 75% by weight, preferably greater than 80% by weight, more preferably greater than 85% by weight, particularly and preferably greater than 90% by weight, in each case based on the total weight of the composition, and preferably - 90% by weight or less, more preferably 75% by weight or less, even more preferably 50% by weight or less, particularly and preferably 25% by weight or less, especially and preferably 10% by weight or less , and more preferably 5% by weight or less of liquid diluents (at 25 ° C and 101.3 kPa (1013 mbar)), in particular water, in each case based on the total weight of the composition, and / or - a total amount of 5% by weight or less, more preferably 2% by weight or less, particularly and preferably 1.25% by weight or less, and more preferably 0.8% by weight or less of proteins, in each case based on the total weight of the composition, and / or - a total amount of 5% by weight or less, more preferably 3% by weight or less, particularly and preferably 2% by weight or less of sugar and mono- or disaccharide alcohols, in each case based on the total weight composition, and / or - a total amount of 5 wt% or less, more preferably 4 wt% or less, even more preferably 3 wt% or less, particularly and preferably 2 wt% or less, more preferably 1 wt% weight or less of cells and cell material with a size in at least one dimension of more than 3 micrometers (m), preferably with a size in at least one dimension of more than 2 μm, more preferably with a size in at least at least one dimension of more than 1 μm, of fungi of the family Dacrymycetaceae, in each case based on the total weight of the composition.
[0369] [000369] In a particularly preferred embodiment, the present invention relates to a composition which comprises or consists of one or more, preferably two, three, four, five, six, seven, eight, nine, ten, eleven, twelve or more physiologically acceptable salts of one or more, preferably two, three, four, five, six, seven, eight, nine, ten, eleven, twelve or more compounds of formula I, wherein the total amount of said physiologically acceptable salts of the compounds of formula I is greater than 70% by weight, preferably greater than 80% by weight, more preferably greater than 90% by weight, particularly and preferably greater than 95 % by weight, in each case based on the total amount of compounds of formula I and their physiologically acceptable salts, and preferably - a total amount of 90% by weight or less, more preferably 75% by weight or less, even more preferably 50% by weight or less, particularly and preferably 25% by weight or less, special and preferably 10% by weight or less, and more preferably 5% by weight or less of liquid diluents (at 25 ° C and 101.3 kPa (1013 mbar)), in particular water, in each case based on the total weight of the composition, and / or - a total amount of 5% by weight or less, more preferably 3% by weight or less, particularly and preferably 2% by weight or less, and more preferably 1% by weight or less of proteins, in each case with based on the total weight of the composition, and / or - a total amount of 20% by weight or less, more preferably 15% by weight or less, particularly and preferably 10% by weight or less, more preferably 5% by weight or less of sugar alcohols and mono- or disaccharides, in each case based on the total weight of the composition, and / or - a total amount of 5 wt% or less, more preferably 4 wt% or less, even more preferably 3 wt% or less, particularly and preferably 2 wt% or less, more preferably 1 wt% weight or less of cells and cell material with a size in at least one dimension of more than 10 micrometers μm), preferably with a size in at least one dimension of more than 5 μm, more preferably with a size of at least a dimension of more than 3 μm, more preferably with a size of at least one dimension of more than 2 μm, of fungi of the family Dacrymycetaceae, in each case based on the total weight of the composition.
[0370] - uma quantidade total de 25% em peso ou menos, preferivelmente de 10% em peso ou menos, e particular e preferivelmente de 5% em peso ou menos de água, em cada caso com base no peso total da composição, - uma quantidade total de 2% em peso ou menos, preferivelmente de 1 % em peso ou menos de proteínas, em cada caso com base no peso total da composição, - uma quantidade total de 10% em peso ou menos, mais preferivelmente de 5% em peso ou menos de álcoois de açúcares e mono- ou dissacarídeos, em cada caso com base no peso total da composição, e - uma quantidade total de 2% em peso ou menos, mais preferivelmente de 1% em peso ou menos de células e material de célula com um tamanho em pelo menos uma dimensão maior do que 2 μm, mais preferivelmente com um tamanho em pelo menos uma dimensão de mais do que 1 μm, mais preferivelmente com um tamanho em pelo menos uma dimensão de mais do que 0,7 μm de fungos da família Dacrymycetaceae, em cada caso com base no peso total da composição. [000370] In an especially preferred embodiment, the present invention relates to a composition that comprises or consists of three, four, five, six, seven, eight, nine, ten, eleven, twelve or more physiologically acceptable salts of one or more more, preferably two, three, four, five, six, seven, eight, nine, ten, eleven, twelve or more compounds of formula I, wherein the total amount of said physiologically acceptable salts of the compounds of formula I is greater than 85% by weight, more preferably greater than 90% by weight, particularly and preferably greater than 95% by weight, in each case based on the total amount of compounds of formula I and their physiologically acceptable salts, and additionally - a total amount of 25% by weight or less, preferably 10% by weight or less, and particularly and preferably 5% by weight or less of water, in each case based on the total weight of the composition, - a total amount of 2% by weight or less, preferably 1% by weight or less of proteins, in each case based on the total weight of the composition, - a total amount of 10% by weight or less, more preferably 5% by weight or less of sugar and mono- or disaccharide alcohols, in each case based on the total weight of the composition, and - a total amount of 2% by weight or less, more preferably 1% by weight or less of cells and cell material with a size in at least one dimension greater than 2 μm, more preferably with a size in at least one dimension of more than 1 μm, more preferably with a size in at least one dimension of more than 0.7 μm of fungi of the family Dacrymycetaceae, in each case based on the total weight of the composition.
[0371] [000371] Preferably, the determination of the total amount of nitrogen and the total protein content is carried out according to the Kjeldahl method, preferably according to ISO 5549: 1978.
[0372] [000372] It has been found in our own investigations that said preferred or particularly preferred compositions according to the present invention, which comprise one or more physiologically acceptable salts of one or more compounds of formula I, show improved storage stability, in particular , longer compared to the corresponding compounds of formula I in the form of free acid. It has also been found in our own investigations that such compositions have a higher solubility, that is, greater, in aqueous-alcoholic solvents or water, compared to the corresponding compounds of formula I in the form of free acid. For example, although it is not possible to obtain a 1% by weight stable solution of a mixture of compounds of formula I in the form of free acid in water, it is easily possible to produce a 10% by weight solution of a mixture of compounds of formula I in its salt form. Accordingly, the one or more physiologically acceptable salts of one or more compounds of formula I have excellent formulation properties, in particular, with respect to aqueous food, beverage and cosmetic materials, in particular with a water content of 50% in weight or more, which are higher compared to the corresponding compounds of formula I in the form of free acid. Additionally, said compositions and solutions have shown very good skin compatibility.
[0373] [000373] Particularly and preferably, the carbohydrate portion of trisaccharide R in the compounds of formula I - without including any substituents resulting from the acylation of hydroxyl groups of said carbohydrate portion of trisaccharide R - is a beta-D-glycopyranosyl- ( 1⟶ 2) -beta-D-xylopyranosil- (1⟶ 2) -beta-D-xylopyranoside. In addition, of said trisaccharide carbohydrate portion R, preferably one, two, three or four hydroxyl groups are esterified by a C2-C10-alkanoic acid, i.e., said trisaccharide carbohydrate portion R being in mono- or di-form - or tri- or tetra-acylated (that is, esterified with a C2-C10-alkanoic acid). It was found in our own investigations that compounds of formula I without any acyl substituents in the carbohydrate portion of trisaccharide R (such as compound [16]) showed significantly lower antimicrobial activities, particularly with respect to Gram-positive bacteria, but also, to a remarkable extent, in relation to yeasts and fungi.
[0374] [000374] Therefore, a mixture according to the present invention, an extract according to the present invention, a material according to the present invention, and / or a composition according to the present invention preferably comprises less than 15% by weight of compounds of formula I without any acyl substituents in the carbohydrate portion of trisaccharide R (particularly compound [16]), more preferably less than 10% by weight, particularly and preferably less than 5% by weight each case based on the total amount of compounds of formula I and their physiologically acceptable salts.
[0375] [000375] A particularly preferred embodiment relates to the compounds of formula I which carry one, two, three or four acylated hydroxyl groups in the carbohydrate moiety of trisaccharide R, said acyl moiety preferably being a C3-C6-alkanoic acid, more preferably a portion of isovaleryl (3-methyl-butanoyl). These compounds have been found to exhibit superior antimicrobial activity in relation to certain Gram-positive bacteria and in relation to fungi, yeasts and molds.
[0376] [000376] Preferably, such mixture comprises, essentially consists of or consists of alkaline and / or alkaline earth salts of two or more compounds of formula I, more preferably of sodium and / or potassium and / or calcium and / or magnesium salts, in particular the sodium and / or potassium and / or calcium and / or magnesium salts of one, two, three, four, five, six, seven, eight, nine or more of the compounds selected from the group consisting of compounds [1 ], [4], [5], [6], [7], [8], [9], [10], [12], [13], [14], and [18], where the amount total water is preferably less than 5% by weight, more preferably less than 3% by weight, and particularly and preferably less than 1% by weight, in each case based on the total weight of the mixture.
[0377] [000377] Preferably such a mixture comprises the sodium and / or potassium and / or calcium and / or magnesium salts of one, two, three, four, five, six, seven, eight or more of the compounds selected from the group consisting of in [1], [4], [5], [6], [7], [8], [9], [10], [12], [13], [14], and [18], where the total amount of salts of the compounds of formula I is greater than 70% by weight, preferably greater than 75% by weight, more preferably greater than 80%, particularly and preferably greater than 85% by weight, in each case based on the total weight of the mixture.
[0378] [000378] Particularly and preferably such a mixture comprises the sodium and / or potassium and / or calcium and / or magnesium salts of one, two, three, four or all compounds selected from the group consisting of compounds 1], [7 ], [12], [13], and [18], where the total amount of said salts of compounds [1], [7], [12], [13], and [18] is greater than 10% by weight, preferably greater than 15% by weight, more preferably greater than 20% by weight, in each case based on the total weight of the mixture.
[0379] [000379] Preferably, such a mixture according to the present invention is in solid form (at 25 ° C and 101.3 kPa (1013 mbar)), preferably in powder form with a total residual water content of 5% weight or less, preferably 3% by weight or less, more preferably 1% by weight or less, or is in the form of an aqueous or aqueous-alcoholic solution, where the total amount of compounds of formula I or their physiologically salts acceptable values is in the range of 1 to 40% by weight, more preferably in the range of 2 to 33% by weight, even more preferably in the range of 3 to 25% by weight, and most preferably still in the range of 5 to 20% by weight , in each case based on the total weight of the mixture.
[0380] [000380] Further purification of an extract according to the present invention, a mixture according to the present invention and, in particular, a composition comprising or consisting of one or more physiologically acceptable salts of one or more compounds of formula I (as defined above) can be further purified according to the methods and materials described in US 6.051.212, WO 96/38057 and / or JP 2006-176438 A, preferably centrifugation and / or filtration (including ultrafiltration and / or microfiltration), preferably using one or more sorption materials (absorbents or adsorbents) selected from the group consisting of activated carbons, charcoal, ion exchange resins (preferably a weakly basic or weakly acidic ion exchange resin, resins macroporous ion exchange agents, in turn, being preferred), silica, alumina, filter soil (diatomaceous earth, for example, celite), glass particles, glass wool, fiber glass, zeolites (such as zeolite A, zeolite X, zeolite Y), silicates and aluminosilicates (preferably clays and clay minerals, such as bentonite, kaolinite, montmorillonite, smectite, illite, chlorite).
[0381] - preferivelmente proporcionar um fungo da família Dacrymycetaceae, preferivelmente um fungo dos gêneros Dacryopinax, Dacrymyces, Ditiola, Femsjonia ou Guepiniopsis, mais preferivelmente um fungo da espécie Dacryopinax spathularia, - realizar um processo de fermentação de modo tal que um, dois ou mais compostos de fórmula I sejam produzidos (preferivelmente pelo dito fungo da família Dacrymycetaceae), - ajustar o valor do pH do caldo de fermentação para um valor abaixo de 4, preferivelmente um valor de pH na faixa de 1 a 3,5, mais preferivelmente para um valor de pH na faixa de 1,5 a 3, - manter a mistura de reação resultante em um valor de pH abaixo de 4, preferivelmente em um valor de pH na faixa de 1 a 3,5, mais preferivelmente até um valor de pH na faixa de 1,5 a 3, com isso precipitando parcial ou essencialmente um, dois ou mais compostos de fórmula I, e - lavar o precipitado resultante compreendendo, consistindo essencialmente em ou consistindo em um, dois ou mais compostos de fórmula I, preferivelmente com um diluente aquoso, mais preferivelmente com a água, particular e preferivelmente com a água desmineralizada, - opcionalmente suspender o precipitado compreendendo, essencialmente consistindo em ou consistindo em um, dois ou mais compostos de fórmula I em um diluente aquoso, mais preferivelmente na água, particular e preferivelmente na água desmineralizada, - opcionalmente remover a água do produto resultante preferivelmente liofilizando o produto resultante, com isso preferivelmente produzindo um extrato compreendendo, essencialmente consistindo em ou consistindo em um, dois ou mais compostos de fórmula I na forma sólida (e de preferência, essencialmente sem água). [000381] In a further aspect, the present invention relates to a method of producing an extract comprising a compound of formula I or a mixture of two or more compounds of formula I (for use) according to the present invention, in particular, a mixture comprising one or more compounds selected from the group consisting of compounds [1], [2], [3], [4], [5], [6], [7], [8] , [9], [10], [11], [12], [13], [14], [18], [19], [20], and [21], comprising the following steps: - preferably providing a fungus of the family Dacrymycetaceae, preferably a fungus of the genera Dacryopinax, Dacrymyces, Ditiola, Femsjonia or Guepiniopsis, more preferably a fungus of the species Dacryopinax spathularia, - carry out a fermentation process in such a way that one, two or more compounds of formula I are produced (preferably by the said fungus of the family Dacrymycetaceae), - adjust the pH value of the fermentation broth to a value below 4, preferably a pH value in the range of 1 to 3.5, more preferably to a pH value in the range of 1.5 to 3, - maintain the resulting reaction mixture at a pH value below 4, preferably at a pH value in the range of 1 to 3.5, more preferably up to a pH value in the range of 1.5 to 3, thereby partially or essentially precipitating one, two or more compounds of formula I, and - washing the resulting precipitate comprising, consisting essentially of or consisting of one, two or more compounds of formula I, preferably with an aqueous diluent, more preferably with water, particularly and preferably with demineralized water, - optionally suspending the precipitate comprising, essentially consisting of or consisting of one, two or more compounds of formula I in an aqueous diluent, more preferably in water, particularly and preferably in demineralized water, - optionally removing water from the resulting product preferably by lyophilizing the resulting product, thereby preferably producing an extract comprising, essentially consisting of or consisting of one, two or more compounds of formula I in solid form (and preferably, essentially without water).
[0382] - proporcionar um fungo da família Dacrymycetaceae, preferivelmente um fungo dos gêneros Dacryopinax, Dacrymyces, Ditiola, Femsjonia ou Guepiniopsis, mais preferivelmente um fungo da espécie Dacryopinax spathularia, - realizar um processo de fermentação de modo tal que um composto de fórmula I ou uma mistura de dois ou mais compostos de fórmula I seja produzida pelo dito fungo, - ajustar o valor do pH do caldo de fermentação para um valor abaixo de 4, preferivelmente para um valor de pH na faixa de 1 a 3,5, mais preferivelmente para um valor de pH na faixa de 1,5 a 3, - manter a mistura de reação resultante em um valor de pH abaixo de 4, preferivelmente em um valor de pH na faixa de 1 a 3,5, mais preferivelmente até um valor de pH na faixa de 1,5 a 3, com isso precipitando parcial ou essencialmente um composto de fórmula I ou uma mistura de dois ou mais compostos de fórmula I, - preferivelmente lavar o precipitado (pelota) resultante preferivelmente com um diluente aquoso, mais preferivelmente com a água, particular e preferivelmente com a água desmineralizada, - suspender o precipitado (pelota) resultante em um diluente aquoso, mais preferivelmente na água, particular e preferivelmente na água desmineralizada, - ajustar o valor do pH da suspensão para um valor na faixa de 4,5 a 7,5, preferivelmente na faixa de 5 a 7, particular e preferivelmente na faixa de 5,5 a 6,5, por adição de uma base inorgânica (solução ou suspensão), preferivelmente o hidróxido de sódio, o hidróxido de potássio, o hidróxido de cálcio, e/ou o hidróxido de magnésio, e - remover a água do produto resultante, preferivelmente secando, particularmente liofilizando o produto resultante, com isso obtendo um ou mais sais fisiologicamente aceitáveis de um ou mais compostos de fórmula I de acordo com a presente invenção como um sólido, preferivelmente um pó, e de preferência na forma essencialmente sem água. [000382] In a further aspect, the present invention relates to a method of producing one or more physiologically acceptable salts of one or more compounds of formula I according to the present invention, in particular one or more physiologically acceptable salts of a , two or more compounds selected from the group consisting of compounds [1], [2], [3], [4], [5], [6], [7], [8], [9], [10], [11], [12], [13], [14], [18], [19], [20], and [21], comprising the following steps: - provide a fungus of the family Dacrymycetaceae, preferably a fungus of the genera Dacryopinax, Dacrymyces, Ditiola, Femsjonia or Guepiniopsis, more preferably a fungus of the species Dacryopinax spathularia, - carrying out a fermentation process in such a way that a compound of formula I or a mixture of two or more compounds of formula I is produced by said fungus, - adjust the pH value of the fermentation broth to a value below 4, preferably to a pH value in the range of 1 to 3.5, more preferably to a pH value in the range of 1.5 to 3, - maintaining the resulting reaction mixture at a pH value below 4, preferably at a pH value in the range of 1 to 3.5, more preferably up to a pH value in the range of 1.5 to 3, thereby precipitating partially or essentially a compound of formula I or a mixture of two or more compounds of formula I, - preferably washing the resulting precipitate (pellet) preferably with an aqueous diluent, more preferably with water, particularly and preferably with demineralized water, - suspend the resulting precipitate (pellet) in an aqueous diluent, more preferably in water, particularly and preferably in demineralized water, - adjust the pH value of the suspension to a value in the range of 4.5 to 7.5, preferably in the range of 5 to 7, particularly and preferably in the range of 5.5 to 6.5, by adding an inorganic base (solution or suspension), preferably sodium hydroxide, potassium hydroxide, calcium hydroxide, and / or magnesium hydroxide, and - removing water from the resulting product, preferably by drying, particularly freeze-drying the resulting product, thereby obtaining one or more physiologically acceptable salts of one or more compounds of formula I according to the present invention as a solid, preferably a powder, and of preferably in the form essentially without water.
[0383] [000383] Preferably, fermentation is carried out in the absence of an effective amount of visible light (i.e. light with a wavelength in the range of 380 to 750 nm), more preferably in the absence of an effective amount of visible light and ultraviolet light, more preferably in the absence of an effective amount of light. Due to this measure, the production of carotenoids, in particular beta-carotene, is minimized or avoided (in contrast to US 2,974,044).
[0384] [000384] Preferably, fermentation is carried out with one or more fungi selected from the group consisting of Dacryopinax aurantiaca, Dacryopinax crenata, Dacryopinax dennisii, Dacryopinax elegans, Dacryopinax felloi, Dacryopinax fissus, Dacryopinax foliacea, Dacryopinax formosus, imazekiana Dacryopinax, Dacryopinax indacocheae, Dacryopinax lowyi, Dacryopinax macrospora, Dacryopinax martinii, Dacryopinax maxidorii, Dacryopinax parmastoensis, Dacryopinax petaliformis, Dacryopinax spathularia , Dacryopinax sphenocarpa, Dacryopinax taibaishanensis, Dacryopinax xizangensis, Dacryopinax yungensis, Dacrymyces ancyleus, Dacrymyces aureosporus, Dacrymyces australis Dacrymyces capitatus, Dacrymyces chrysocomus, Dacrymyces chrysospermus, Dacrymyces cupularis, Dacrymyces dictyosporus, Dacrymyces enatus, Dacrymyces flabelliformis, Dacrymyces intermedius, Dacrymyces lacrymalis, Dacrymyces macnabbii, Dacrymyces minor, Dacrymyces novae-zelandiae, Dacrymyces ovisporus, Dacrymyces paraphysatus, Dacrymyces pinacearum, Dacrymyces punctiformis, Dacrymyces stillatus, Dacrymyces subarcticus, Dacrymyces tortus, Dacrmymyces tortus ymyces variisporus, Ditiola abieticola, Ditiola brasiliensis, Ditiola coccinea, Ditiola nuda, Ditiola oblique, Ditiola orientalis, Ditiola pezizaeformis, Ditiola radicata, Guepiniopsis alpina, Guepiniopsis, Gispiniopsis, Gispiniopsis, Gispiniopsis, Gispiniopsis,
[0385] [000385] In a preferred embodiment, a material according to the present invention comprising a total amount of water defined above (preferred or particularly preferred) is selected from the group consisting of an O / W emulsion, a hydrodispersion, a suspension, a solution, or a hydrogel.
[0386] [000386] Preferably, a material according to the present invention comprises a high proportion of water, preferably water in a total amount of 50% by weight or more, more preferably 60% by weight or more, even more preferably 65% by weight or more, particularly and preferably 70% by weight or more, and more preferably still 75% by weight or more, in each case based on the total weight of the material.
[0387] [000387] Preferably, the total amount of water is in the range of 70 to 99.5% by weight, more preferably in the range of 75 to 99% by weight, and most preferably still in the range of 80 to 98% by weight, in each case based on the total weight of the material.
[0388] [000388] The total amount of water of an orally consumable material according to the present invention, in particular a ready-to-drink composition according to the present invention, is 60% by weight or more, preferably 70% by weight or more , more preferably 75% by weight or more, even more preferably 80% by weight or more, in each case based on the total weight of the orally consumable material.
[0389] [000389] Preferably, the total amount of water, preferably non-deionized water, particularly drinking water or mineral water, of an orally consumable material according to the present invention, in particular of a ready-to-drink composition according to the present invention, is in the range of 82% by weight to 98% by weight, more preferably in the range of 83% by weight to 96% by weight, even more preferably in the range of 84% by weight to 95% by weight, and more preferably still in the range of 85% by weight to 94% by weight, in each case based on the total weight of the orally consumable material.
[0390] [000390] In a preferred embodiment, a material according to the present invention comprising a total amount of water (preferred or particularly preferred) defined above has a pH value at 25 ° C of 6.8 or less, preferably in the range of 1.5 to 6.5, more preferably in the range of 2.0 to 6.0, even more preferably in the range of 2.1 to 5.8, particularly and preferably in the range of 2.2 to 5.0, and more preferably still in the range of 2.3 to 4.5.
[0391] [000391] The pH value of an orally consumable material according to the present invention, in particular a ready-to-drink composition according to the present invention, when measured at 25 ° C (and preferably at 101.3 kPa (1013 mbar)), preferably is in the range of 1.5 to 6.5, preferably in the range of 1.8 to 6.0, more preferably in the range of 2.0 to 5.5, even more preferably in the range of 2, 0 to 5.0, particularly and preferably in the range of 2.1 to 4.4, especially and preferably in the range of 2.2 to 4.2, and more preferably still in the range of 2.3 to 3.9.
[0392] [000392] It should be emphasized that the one or more compounds of the aforementioned formula I, one or more physiologically acceptable salts of a compound of the aforementioned formula I, or a mixture of them are stable (preferably at temperatures of 40 ° C or less) for a long period of time (generally more than 16 weeks at 25 ° C) in such aqueous materials at the acidic pH values indicated above, that is, the compounds of the aforementioned formula I are not decomposed or degraded to an appreciable extent or significant.
[0393] - um ou mais ácidos orgânicos alimentícios e/ou o sal fisiologicamente aceitável dos mesmos, preferivelmente selecionados a partir do grupo consistindo em ácido acético, ácido adípico, ácido cafeotânico, ácido cítrico, ácido isocítrico, ácido maléico, ácido fumárico, ácido galacturônico, ácido glicurônico, ácido glicérico, ácido glicólico, ácido láctico, ácido málico, ácido oxálico, ácido pirúvico, ácido quínico, ácido succínico, ácido tânico, ácido tartárico, e os sais fisiologicamente aceitáveis dos mesmos, preferivelmente os sais dos mesmos de sódio e/ou potássio e/ou cálcio e/ou magnésio,e/ou - um ou mais ácidos inorgânicos comestíveis e/ou o sal fisiologicamente aceitável dos mesmos, preferivelmente selecionados a partir do grupo consistindo em ácido fosfórico, ácido pirofosfórico, ácidos polifosfóricos, e ácidos bisfosfônicos (em particular aqueles explicitamente mencionados no parágrafo [0050] da US 2010/0151104 A1) e os sais fisiologicamente aceitáveis dos mesmos,e/ou - um ou mais adoçantes de alta potência, preferivelmente selecionados a partir do grupo consistindo em magap, ciclamato de sódio, acessulfame K, neo-hesperidina di-hidrocalcona, sal sódico de sacarina, aspartame, superaspartame, neotame, alitame, sucralose, esteviosídeo, rebaudiosídeos (preferivelmente rebaudiosídeo A), lugduname, carrelame, sucrononato, sucro-octato, monatina, filodulcina, hernandulcina, di-hidrocalcona glicosídeos, glicirrizina, ácido glicirretínico e seus sais fisiologicamente aceitáveis de sabor doce, mogrosídeos, extratos de alcaçuz (Glycyrrhizza glabra ssp.), extratos de Lippia dulcis, extratos de Momordica ssp. (em particular Momordica grosvenori [Luo Han Guo]), extratos de Hydrangea dulcis e extratos de Stevia ssp. (por exemplo, Stevia rebaudiana),preferivelmente um ou mais adoçantes de alta potência em uma quantidade total isodoce a, ou mais doce do que, uma solução a 1,0% em peso de sacarose em água, mais preferivelmente em uma quantidade total isodoce a, ou mais doce do que, uma solução a 2,0% em peso de sacarose em água, ainda mais preferivelmente em uma quantidade total isodoce a, ou mais doce do que, uma solução a 3,0% em peso de sacarose em água, particular e preferivelmente em uma quantidade total isodoce a, ou mais doce do que, uma solução a 4,0% em peso de sacarose em água, e/ou - um ou mais mono- ou dissacarídeos de sabor doce, preferivelmente selecionados a partir do grupo consistindo em sacarose, lactose, maltose, glicose, e frutose, a quantidade total dos ditos mono- ou dissacarídeos de sabor doce estando na faixa de 1,5 a 19% em peso, preferivelmente na faixa de 2,5 a 16% em peso, mais preferivelmente na faixa de 3,5 a 14% em peso, particular e preferivelmente na faixa de 4,5 a 13% em peso, e mais preferivelmente na faixa de 5,5 a 12% em peso, em cada caso com base no peso total do material, e preferivelmente um, dois, três, quatro, cinco ou mais agentes aromatizantes, preferivelmente tendo um peso molecular na faixa de 120 a 300 g/mol, mais preferivelmente na faixa de 130 a 280 g/mol.[000393] In a preferred embodiment, a material according to the present invention (preferably comprising a preferred or particularly preferred total amount of water defined above and having a pH value in a preferred or particularly preferred range indicated above), suitable for the oral consumption, preferably a food or drink, comprises - one or more organic food acids and / or the physiologically acceptable salt thereof, preferably selected from the group consisting of acetic acid, adipic acid, caffeic acid, citric acid, isocitric acid, maleic acid, fumaric acid, galacturonic acid, glycuronic acid, glycolic acid, glycolic acid, lactic acid, malic acid, oxalic acid, pyruvic acid, quinic acid, succinic acid, tannic acid, tartaric acid, and their physiologically acceptable salts, preferably the same and / or sodium salts potassium and / or calcium and / or magnesium, and / or - one or more edible inorganic acids and / or the physiologically acceptable salt thereof, preferably selected from the group consisting of phosphoric acid, pyrophosphoric acid, polyphosphoric acids, and bisphosphonic acids (in particular those explicitly mentioned in US paragraph [0050] 2010/0151104 A1) and the physiologically acceptable salts thereof, and / or - one or more high potency sweeteners, preferably selected from the group consisting of magap, sodium cyclamate, acesulfame K, neo-hesperidin dihydrocalcone, saccharin sodium salt, aspartame, superaspartame, neotame, alitame, sucralose, stevioside, rebaudioside (preferably rebaudioside A), lugduname, carrelame, sucrononate, sucro-octate, monatin, phyllodulcin, hernandulcin, dihydrocalconate glycosides, glycyrrhizin, glycyrrhetinic acid and its physiologically acceptable salts of sweet, ghroscyrous, extracts .), extracts of Lippia dulcis, extracts of Momordica ssp. (in particular Momordica grosvenori [Luo Han Guo]), extracts of Hydrangea dulcis and extracts of Stevia ssp. (e.g. Stevia rebaudiana), preferably one or more high potency sweeteners in a total amount of isodoce a, or sweeter than, a 1.0 wt% solution of sucrose in water, more preferably in a total amount of isodoce a, or sweeter than, a 2.0% by weight solution of sucrose in water, even more preferably in a total amount is greater than, or sweeter than, a 3.0% solution by weight of sucrose in water, particularly and preferably in an amount total isodoce to, or sweeter than, a 4.0% by weight solution of sucrose in water, and / or - one or more sweet-flavored mono- or disaccharides, preferably selected from the group consisting of sucrose, lactose, maltose, glucose, and fructose, the total amount of said sweet-flavored mono- or disaccharides being in the range of 1.5 to 19% by weight, preferably in the range of 2.5 to 16% by weight, more preferably in the range of 3.5 to 14% by weight, particularly and preferably in the range of 4.5 to 13% by weight, and more preferably in the range of 5.5 to 12% by weight, in each case based on the total weight of the material, and preferably one, two, three, four, five or more flavoring agents, preferably having a molecular weight in the range of 120 to 300 g / mol, more preferably in the range of 130 to 280 g / mol.
[0394] [000394] The one or more compounds of formula I and / or the physiologically acceptable salts thereof, especially as defined in one of the preferred or particularly preferred embodiments, and mixtures as defined in one of the preferred or particularly preferred embodiments allow the prevention of deterioration of materials with such a high proportion of water (and preferably a pH value at 25 ° C in the range of 2.2 to 4.6) by microorganisms, in a sealed container, for a period of at least 12 weeks , preferably at least 16 weeks, at 25 ° C. Thus, it is possible to reduce or replace conventional preservatives (which may present health and / or environmental concerns).
[0395] [000395] The one or more compounds of formula I and / or the physiologically acceptable salts thereof, especially as defined in one of the preferred or particularly preferred embodiments, and the mixtures as defined in one of the preferred or particularly preferred embodiments, can be used together in other known beverage preservatives, in an additive or synergistic way, to reduce the amount of preservative required and thereby improve the sensory impact of the inventive drink on drinks having conventional preservatives. Such other known beverage preservatives are preferably selected from the group consisting of ethyl-N-alpha-lauroyl-L-arginate (LAE) and its hydrochloride, dimethyl dicarbonate, trans-cinnamic acid, EDTA (ethylenediaminetetraacetic acid) and physiologically acceptable salts thereof, preferably their sodium and / or calcium salts, EDDS (ethylene diamine-N, N'-disuccinic acid) and physiologically acceptable salts thereof, preferably the sodium and / or calcium salts, polyphosphoric acid and physiologically acceptable salts thereof (preferably comprising or consisting of sodium hexametaphosphate), bisphosphonic acids and bisphosphonates (in particular, those explicitly mentioned in paragraph [0050] of US 2010/0151104 A1), and mixtures thereof.
[0396] [000396] The total amount of ethyl-N-alpha-lauroyl-L-arginate and its hydrochloride is preferably in the range of 1 to 25 ppm, more preferably 2 to 12 ppm, based on the total weight of the drink.
[0397] [000397] The total amount of dimethyl dicarbonate is preferably in the range of 20 to 500 ppm, more preferably 50 to 250 ppm, based on the total weight of the drink.
[0398] [000398] The total amount of trans-cinnamic acid is preferably in the range of 1 to 40 ppm, more preferably 2 to 30 ppm, based on the total weight of the beverage.
[0399] [000399] The total amount of EDTA (ethylene diaminetetraacetic acid) and physiologically acceptable salts is in the range of 0.5 to 50 ppm, more preferably 1 to 30 ppm, based on the total weight of the drink.
[0400] [000400] The total amount of EDDS (ethylene diamine-N, N'-disuccinic acid) and physiologically acceptable salts thereof is in the range of 1 to 500 ppm, more preferably 20 to 450 ppm, based on the total weight of the drink.
[0401] [000401] The total amount of polyphosphonic acid and physiologically acceptable salts thereof is in the range of 10 to 1500 ppm, based on the total weight of the drink.
[0402] [000402] As already mentioned above, compounds of formula I, preferably compounds [1], [2], [3], [4], [5], [6], [7], [8], [ 9], [10], [11], [12], [13], [14], [18], [19], [20], [21], and their physiologically acceptable salts do not exhibit a flavor characteristic or unpleasant sensation in the mouth, in particular no unpleasant taste, in particular in the total quantities used in the ready-to-use product.
[0403] [000403] The total amount of the compounds of formula I, preferably of the compounds [1], [2], [3], [4], [5], [6], [7], [8], [9] , [10], [11], [12], [13], [14], [18], [19], [20], [21] and the physiologically acceptable salts thereof, more preferably the compounds [1 ], [4], [5], [6], [7], [8], [9], [10], [12], [13], [14], [18], and of the physiologically salts acceptable values thereof, preferably is in the range of 0.1 to 1000 ppm, more preferably from 0.5 to 500 ppm, particularly and preferably from 1 to 250 ppm, and most preferably from 2 to 150 ppm, in each case based in the total weight of the orally consumable material according to the present invention, particularly a food or drink according to the present invention.
[0404] [000404] Therefore, the compounds of formula I and the physiologically acceptable salts thereof are preferably used in orally consumable materials, particularly food or drink, according to the invention, in combination with one or more flavoring agents, preferably having a molecular weight in the range of 120 to 300 g / mol, more preferably in the range of 130 to 280 g / mol.
[0405] [000405] Preferably, one, two, three or more of said flavoring agents are fresh, sweet, tasty, spicy and / or herbaceous flavoring agents, preferably selected from the group consisting of menthol (preferably L-menthol, D-menthol , racemic menthol, isomentol, neoisomentol, neomenthol), isomentone, menthol, peppermint oil, L-carvone, D-carvone, peppermint oil, cineol, eucalyptus oil, cinnamaldehyde (preferably trans-cinnamaldehyde), cinnamic alcohol, cinnamon bark oil, cinnamon leaf oil, methyl cinnamate, benzaldehyde, furfural, furfuryl alcohol, methyl salicylate, wintergreen oil, thyme oil, thymol, carvacrol, clove oil, camphene, p -cymene, alpha-terpinene, borneol, eugenol, fennel oil, star anise oil, anethole (preferably trans-anethole), anisole, cis-3-hexenol, cis-3-hexenyl acetate, D-limonene , L-limonene, linalool, citral, geraniol, geranyl acetate, nerol, citronelol, citrone lal, alpha-felandrene, beta-felandrene, alpha-pinene, beta-pinene, vanilla extract, vanilla, ethyl vanilla, 2-hydroxy-4-methoxybenzaldehyde, 2,5-dimethyl-4-hydroxy-3 (2H) -furanone , 2-ethyl-4-hydroxy-5-methyl-3 (2H) -furanone, 2-ethyl-5-methyl-4-hydroxy-3 (2H) -furanone, 5-ethyl-2-methyl-4-hydroxy -3 (2H) -furanone, 3-hydroxy-4,5-dimethyl-2 (5H) -furanone, maltol, ethylmaltol, coumarin, butyrolactone, gamma-undecalactone, gamma-nonalactone, 4-methyl-delta-lactone, lactone massoia, sotolone, delta-decalactone, tuberolactone, methyl sorbate, 2-hydroxy-3-methyl-2-cyclopentenones, n-butyl acetate, isoamyl acetate, ethyl butyrate, n-butyl butyrate, isoamyl butyrate , Ethyl 3-methyl-butyrate, ethyl n-hexanoate, allyl n-hexanoate, n-butyl n-hexanoate, ethyl n-octanoate, ethyl-3-methyl-3-phenylglycidate, ethyl-2-trans -4-cis-decadienoate, 4- (p-hydroxyphenyl) -2-butanone, 1,1-dimeoxy-2,2,5-trimethyl-4-hexane, 2,6-dimethyl-5-hepten-1 -al, and phenylacetaldehyde.
[0406] [000406] Preferably, one, two, three or more of said flavoring agents are sweet, tasty and / or seasoned flavoring agents, preferably selected from the group consisting of trans-cinnamaldehyde, cinnamic alcohol, methyl cinnamate, benzaldehyde, furfural , furfuryl alcohol, camphene, p-cymene, alpha-terpinene, borneol, eugenol, trans-anethole, anisol, cis-3-hexenol, cis-3-hexenyl acetate, D-limonene, L-limonene, linalool, citral, geraniol, geranyl acetate, nerol, citronelol, citronelal, alpha-felandrene, beta-felandrene, alpha-pinene, beta-pinene, vanilla extract, vanilla, ethyl vanilla, 2-hydroxy-4-methoxybenzaldehyde, 2,5-dimethyl- 4-hydroxy-3 (2H) -furanone, 2-ethyl-4-hydroxy-5-methyl-3 (2H) -furanone, 2-ethyl-5-methyl-4-hydroxy-3 (2H) -furanone, 5 -ethyl-2-methyl-4-hydroxy-3 (2H) -furanone, 3-hydroxy-4,5-dimethyl-2 (5H) -furanone, maltol, ethylmaltol, coumarin, gamma-undecalactone, gamma-nonalactone, 4 -methyl-delta-lactone, massoia lactone, sotolone, delta-decalactone, tuberolactone, methyl sorbate, n-butyl acetate, isoamyl acetate, ethyl butyrate, n-butyl butyrate, isoamyl butyrate, ethyl 3-methyl butyrate, ethyl n-hexanoate, allyl n-hexanoate, n-butyl n-hexanoate, ethyl n-octanoate, ethyl-3-methyl-3-phenylglycidate, ethyl-2-trans-4-cis-decadienoate, 4- (p-hydroxyphenyl) -2-butanone, 2, 6-dimethyl-5-hepten-1-al, and phenylacetaldehyde.
[0407] [000407] The present invention also relates to foods, such as meat, meat products, fish and seafood products, with increased shelf life stability and increased resistance against bacterial growth Gram positive. The preparation process for the manufacture of foodstuffs using the compounds of formula I and / or the physiologically acceptable salts thereof, according to the invention, comprises, for example, the combination of a meat, meat products, fish or product of uncooked seafood with one or more phosphates, one or more lactates, lactic acid, and preferably also and / or a flavoring agent, followed by further processing, such as packaging or cooking. In this specific aspect, the present invention makes use of phosphates that are functional in activating protein in meat, meat products, fish or seafood and also have properties to absorb lactates and flavoring agents.
[0408] [000408] In this context, the present invention relates to a foodstuff treatment composition, particularly for the treatment of meat, meat products, fish and seafood products, said composition comprising (i) one or more compounds of formula I and / or their physiologically acceptable salts, (ii) lactic acid and / or lactates, and (iii) one or more phosphates.
[0409] (i) um, dois, três ou mais dos compostos [1], [2], [3], [4], [5], [6], [7], [8], [9], [10], [11], [12], [13], [14], [18], [19], [20], [21] e/ou dos sais fisiologicamente aceitáveis dos mesmos, preferivelmente em uma quantidade total de 0,0001 a 1 % em peso, mais preferivelmente em uma quantidade total de 0,0005 a 0,5% em peso, ainda mais preferivelmente em uma quantidade total de 0,001 a 0,1% em peso, particular e preferivelmente em uma quantidade total de 25 a 500 ppm, (ii) lactato de sódio e/ou lactato de potássio, preferivelmente em uma quantidade total de 0,5 a 25% em peso, mais preferivelmente em uma quantidade total de 1 a 20% em peso, ainda mais preferivelmente em uma quantidade total de 1,5 a 15% em peso, (iii) um ou mais fosfatos de sódio e/ou potássio selecionados a partir do grupo consistindo em ortofosfatos de sódio e/ou potássio, pirofosfatos (difosfatos), metafosfatos, e polifosfatos (hexametafosfatos), preferivelmente em uma quantidade total de 1 a 45% em peso, mais preferivelmente em uma quantidade total de 2 a 30% em peso, ainda mais preferivelmente em uma quantidade total de 3 a 25% em peso, (iv) preferivelmente a água, mais preferivelmente a água em uma quantidade total de 50% em peso ou mais, ainda mais preferivelmente em uma quantidade total de 60% em peso ou mais, e opcionalmente (v) um ou mais constituintes adicionais selecionados a partir do grupo consistindo em cloreto de sódio, nitrito de sódio, nitrito de potássio, nitrato de sódio, nitrato de potássio, lactato de cálcio, diacetato de sódio, ácido acético, acetato de sódio, diacetato de sódio, acetato de potássio, diacetato de potássiio, ácido cítrico, e citrato de sódio, em que as quantidades em cada caso são baseadas no peso total da composição de tratamento de gênero alimentício. [000409] In a preferred embodiment of the invention, said foodstuff treatment composition comprises (i) one, two, three or more of the compounds [1], [2], [3], [4], [5], [6], [7], [8], [9], [10 ], [11], [12], [13], [14], [18], [19], [20], [21] and / or their physiologically acceptable salts, preferably in a total amount of 0 0001 to 1% by weight, more preferably in a total amount of 0.0005 to 0.5% by weight, even more preferably in a total amount of 0.001 to 0.1% by weight, particularly and preferably in a total amount from 25 to 500 ppm, (ii) sodium lactate and / or potassium lactate, preferably in a total amount of 0.5 to 25% by weight, more preferably in a total amount of 1 to 20% by weight, even more preferably in a total amount of 1.5 to 15% by weight, (iii) one or more sodium and / or potassium phosphates selected from the group consisting of sodium and / or potassium orthophosphates, pyrophosphates (diphosphates), metaphosphates, and polyphosphates (hexametaphosphates), preferably in a total amount of 1 to 45 % by weight, more preferably in a total amount of 2 to 30% by weight, even more preferably in a total amount of 3 to 25% by weight, (iv) preferably water, more preferably water in a total amount of 50% by weight or more, even more preferably in a total amount of 60% by weight or more, and optionally (v) one or more additional constituents selected from the group consisting of sodium chloride, sodium nitrite, potassium nitrite, sodium nitrate, potassium nitrate, calcium lactate, sodium diacetate, acetic acid, sodium acetate, sodium diacetate, potassium acetate, potassium diacetate, citric acid, and sodium citrate, where the amounts in each case are based on the total weight of the foodstuff treatment composition.
[0410] [000410] In another preferred embodiment of the invention, the one or more sodium and / or potassium phosphate salts of constituent (iii) are selected from trisodium phosphate (Na3PO4), tetrasodium pyrophosphate (Na4P2O7), sodium tripolyphosphate (Na5P3O10), tripotassium phosphate (K3PO4), tetrapotassium pyrophosphate (K4P2O7), potassium tripolyphosphate (K5P3O10), and sodium hexametaphosphate (NaPO3) 6.
[0411] [000411] These food treatment compositions are particularly useful for the treatment of meat, meat products, fish and seafood products, to increase the resistance of the foodstuff against the growth of bacteria, in particular of the Listeria genera ( particularly Listeria monocytogenes), Lactobacillus, Clostridia, Micrococcus (particularly Micrococcus luteus), and / or Bacillus (particularly Bacillus cereus), whereby the foodstuff treatment composition is applied to the food in an amount to reach a total amount of 0, 25 to 6% by weight, preferably 0.5 to 4% by weight, of the constituents (ii) lactic acid and lactates and (iii) phosphates in the final treated foodstuff.
[0412] - um ou mais tensoativos não correspondendo à fórmula I como definida no contexto da presente invenção, preferivelmente um ou mais tensoativos selecionados a partir do grupo consistindo em tensoativos aniônicos, tensoativos catiônicos, tensoativos não iônicos, tensoativos anfotéricos (zwitteriônicos), e biotensoativos, e/ou - um ou mais mono-, di- ou trióis tendo 2 a 14 átomos de carbono, preferivelmente um ou mais di- ou trióis tendo 3 a 12 átomos de carbono, onde preferivelmente a quantidade total de mono-, di- e trióis é 1% em peso ou mais, mais preferivelmente na faixa de 1,1 a 30% em peso, e/ou - uma ou mais substâncias de fragrâncias, preferivelmente uma mistura de três, cinco, oito ou mais substâncias de fragrâncias, mais preferivelmente um perfume, preferivelmente substâncias de fragrâncias em uma quantidade total de 0,1 a 3% em peso, mais preferivelmente em uma quantidade total de 0,15 a 2% em peso, ainda mais preferivelmente em uma quantidade total de 0,2 a 1% em peso, em que as porcentagens em cada caso são baseadas no peso total do produto cosmético. [000412] In a preferred embodiment, a material according to the present invention (preferably comprising a preferred or particularly preferred total amount defined above water and having a pH value in a preferred or particularly preferred range indicated above) is a cosmetic product suitable for topical application on the mucous membrane (mucosa) and / or the epidermis of a mammal, preferably in the form of an O / W lotion, a milk, a (hydro) gel, a body care product and / or hair care (such as preferably a shower gel and / or shampoo, a hair conditioning product, or a deodorant), comprises - one or more surfactants not corresponding to formula I as defined in the context of the present invention, preferably one or more surfactants selected from the group consisting of anionic surfactants, cationic surfactants, non-ionic surfactants, amphoteric (zwitterionic) surfactants, and biotensoactives, and / or - one or more mono-, di- or triols having 2 to 14 carbon atoms, preferably one or more di- or triols having 3 to 12 carbon atoms, where preferably the total amount of mono-, di- and triols is 1 % by weight or more, more preferably in the range of 1.1 to 30% by weight, and / or - one or more fragrance substances, preferably a mixture of three, five, eight or more fragrance substances, more preferably a perfume, preferably fragrance substances in a total amount of 0.1 to 3% by weight, more preferably in a total amount of 0.15 to 2% by weight, even more preferably in a total amount of 0.2 to 1% by weight, the percentages in each case being based on the total weight of the cosmetic product.
[0413] [000413] Preferably, one, several or all mono-, di- or triols having 2 to 14 carbon atoms are selected from the group consisting of ethanol, 1-propanol, 2-propanol, ethylene glycol, 1,2 -propylene glycol, glycerol (glycerin), 1,3-propandiol, 2-methyl-1,3-propandiol, trimethylolpropane, 1,2-butandiol, 1,3-butandiol, 1,4-butandiol, 1,2,3 -butanthriol, 1,2,4-butanthriol, 1-pentanol, 2-pentanol, 3-pentanol, 1,2-pentandiol, 1,3-pentandiol, 1,5-pentandiol, 1-hexanol, 2-hexanol, 3 -hexanol, 1,2-hexandiol, 1,3-hexandiol, dipropylene glycol, 1-octanol, 2-octanol, 3-octanol, 1,2-octandiol (caprylyl glycol), 1,3-octandiol, 2-methyl- 5-cyclohexylpentanol, 2-methyl-4-phenyl-2-butanol, 4-methyl-4-phenyl-2-pentanol (dimethyl phenyl 2-butanol), 1-decanol, 2-decanol, 1,2-decandiol , 3- (2-ethylhexyloxy) propane-1,2-diol (ethylhexylglycerin, octoxiglycerin), 1-dodecanol, 2-dodecanol, 1,2-dodecandiol, 1,12-dodecandiol, 1-tetradecanol, 2 -tetradecanol, 1,2-tetradecandiol and 1,14-tetradecandiol, where p referring to the total amount of mono-, di- and triols having 2 to 14 carbon atoms is 0.5% by weight or more, more preferably 1.0% by weight or more, even more preferably 1.25% by weight or more, and preferably it is in the range of 1.25 to 25% by weight, particularly in the range of 1.5 to 20% by weight, in each case based on the total weight of the cosmetic product.
[0414] [000414] Preferably, one, several or all di- or triols having 3 to 12 carbon atoms are selected from the group consisting of 1,2-propylene glycol, glycerol (glycerin), 1,3-propandiol, 2 -methyl-1,3-propandiol, trimethylolpropane (2- (hydroxymethyl) -2-ethylpropane-1,3-diol), 1,2-butandiol, 1,4-butandiol, 1-pentanol, 2-pentanol, 1, 2-pentandiol, 1,5-pentandiol, 1-hexanol, 2-hexanol, 1,2-hexandiol, dipropylene glycol, 1-octanol, 2-octanol, 1,2-octandiol, 2-methyl-4-phenyl-2 -butanol, 4-methyl-4-phenyl-2-pentanol, 1-decanol, 1,2-decandiol, 1-dodecanol, 1,2-dodecandiol, 1,12-dodecandiol, and 3- (2-ethylhexyloxy ) propane-1,2-diol (ethylhexylglycerin), wherein preferably the total amount of di- and triols having 3 to 12 carbon atoms is 0.5% by weight or more, more preferably 1.0% by weight or more, even more preferably it is in the range of 1.25 to 15% by weight, particularly in the range of 1.5 to 10% by weight, in each case based on the total weight of the cosmetic product.
[0415] - tensoativos aniônicos baseados em ânions permanentes (sulfato, sulfonato, fosfato) ou ânions dependentes do pH (carboxilato), preferivelmente sulfatos [sulfatos de alquila, tais como lauril sulfato de amônio, lauril sulfato de sódio (SDS, dodecil sulfato de sódio), sulfatos de éter de alquila, tais como laureth sulfato de sódio, também conhecido como lauril éter sulfato de sódio (SLES), myreth sulfato de sódio], sulfonatos [docusatos, tais como dioctil sulfossuccinato de sódio, fluortensoativos de sulfonato (perfluor-octanossulfonato (PFOS), perfluorbutanossulfonato), alquil benzeno sulfonatos], fosfatos [fosfato de éter de alquil arila, fosfato de éter de alquila], carboxilatos [carboxilatos de alquila, sais de ácidos graxos (sabões), tais como estearato de sódio, lauroil sarcosinato de sódio, fluortensoativos de carboxilato, tais como perfluornonanoato, perfluor-octanoato (PFOA ou PFO)], - tensoativos catiônicos baseados em aminas primárias, secundárias, ou terciárias dependentes do pH (tais como dicloridrato de octenidina, cátions de amônio quaternário, preferivelmente sais de alquiltrimetilamônio (tais como brometo de cetil trimetilamônio (brometo de hexadecil trimetil amônio), cloreto de cetil trimetilamônio, cloreto de cetilpiridínio, cloreto de benzalcônio, cloreto de benzetônio, cloreto de dimetildioctadecilamônio e brometo de dioctadecildimetilamônio), - tensoativos zwitteriônicos (anfotéricos) baseados em aminas primárias, secundárias, ou terciárias ou cátion de amônio quaternário com sulfonatos (tais como(3-[(3-colamidopropil)dimetilamônio]-1-propanossulfonato) (CHAPS), sultaínas (tais como cocamidopropil hidroxissultaína), carboxilatos (por exemplo, a partir de aminoácidos, imino ácidos), betaínas (tais como cocamidopropil betaína), e fosfatos (tais como lecitinas), - tensoativos não iônicos, preferivelmente álcoois graxos (tais como álcool cetílico, álcool estearílico, álcool cetoestearílico (essencialmente consistindo em álcoois cetílicos e estearílicos), álcool oleílico), éteres alquílicos de polioxietileno glicol CH3-(CH2)10-16-(O-C2H4)1-25-OH (tais como éter monododecílico de octaelileno glicol, éter monododecílico de pentaetileno glicol), éteres alquílicos de polioxipropileno glicol CH3-(CH2)10-16-(O-C3H6)1-25-OH, éteres alquílicos de glicosídeo CH3-(CH2)10-16-(O-glucosídeo)1-3-OH (tais como decil glicosídeo, lauril glicosídeo, octil glicosídeo), éteres de polioxietileno glicol octilfenol C8H17-(C6H4)-(O-C2H4)1-25-OH (tais como Triton X-100), éteres de polioxietileno glicol alquilfenol C9H19-(C6H4)-(O-C2H4)1-25-OH (tais como nonoxinol-9), ésteres de alquila de glicerol (tais como laurato de glicerila), ésteres de polioxietileno glicol sorbitan alquila (tais como polissorbatos, preferivelmente polissorbato 20, polissorbato 40, polissorbato 60, polissorbato 65, polissorbato 80, polissorbato 85 e/ou polissorbato 120; estes estão todos comercialmente disponíveis, por exemplo, sob as marcas registradas Canarcel® ou Tween®), ésteres de alquila de sorbitan, cocamida MEA, cocamida DEA, óxido de dodecildimetilamina, copolímeros em blocos de polietileno glicol e polipropileno glicol (tais como poloxâmeros (comercialmente disponíveis, por exemplo, sob a marca registrada Pluronic®)), e sebo amina polietoxilada (POEA), - biotensoativos, preferivelmente soforolipídios, ramnolipídios e os lipídios de manosil-eritritol e lipopeptídeos (os lipopeptídeos preferidos são a clamidocina, a surfactina, a liquenisina G, e os lipopeptídeos do tipo fengicina). [000415] Anionic surfactants, cationic surfactants, non-ionic surfactants, amphoteric (zwitterionic) surfactants, and bioturfactants are preferably selected from - anionic surfactants based on permanent anions (sulphate, sulphonate, phosphate) or pH-dependent anions (carboxylate), preferably sulphates [alkyl sulphates, such as ammonium lauryl sulphate, sodium lauryl sulphate (SDS, sodium dodecyl sulphate), alkyl ether sulfates, such as sodium laureth sulfate, also known as sodium lauryl ether sulfate (SLES), myreth sodium sulfate], sulfonates [docusates, such as sodium dioctyl sulfosuccinate, sulfonate fluorides (perfluor-octane sulfonate ( PFOS), perfluorbutanesulfonate), alkyl benzene sulfonates], phosphates [alkyl aryl ether phosphate, alkyl ether phosphate], carboxylates [alkyl carboxylates, fatty acid salts (soaps), such as sodium stearate, lauroyl sarcosinate sodium, fluoride surfactants of carboxylate, such as perfluornonanoate, perfluoroctanoate (PFOA or PFO)], - cationic surfactants based on pH-dependent primary, secondary, or tertiary amines (such as octenidine dihydrochloride, quaternary ammonium cations, preferably alkyl trimethylammonium salts (such as trimethylammonium cetyl bromide (trimethyl ammonium hexadecyl bromide), trimethyl ammonium chloride , cetylpyridinium chloride, benzalkonium chloride, benzethonium chloride, dimethyldioctadecylammonium chloride and dioctadecyldimethylammonium bromide), - zwitterionic (amphoteric) surfactants based on primary, secondary, or tertiary amines or quaternary ammonium cation with sulfonates (such as (3 - [(3-colamidopropyl) dimethylammonium] -1-propanesulfonate) (CHAPS), sultaines (such as cocamidopropyl hydroxysultaine), carboxylates (for example, from amino acids, imino acids), betaines (such as cocamidopropyl betaine), and phosphates (such as lecithins), - non-ionic surfactants, preferably fatty alcohols (such as cetyl alcohol, stearyl alcohol, ceto-stearyl alcohol (essentially consisting of cetyl and stearyl alcohols), oleyl alcohol), polyoxyethylene glycol CH3- (CH2) 10-16- (O- C2H4) 1-25-OH (such as octaelylene glycol monododecyl ether, pentaethylene glycol monododecyl ether), polyoxypropylene glycol alkyl ethers CH3- (CH2) 10-16- (O-C3H6) 1-25-OH, alkyl ethers glycoside CH3- (CH2) 10-16- (O-glucoside) 1-3-OH (such as decyl glycoside, lauryl glycoside, octyl glycoside), polyoxyethylene glycol ethers octyphenol C8H17- (C6H4) - (O-C2H4) 1-25-OH (such as Triton X-100), polyoxyethylene glycol alkylphenols C9H19- (C6H4) - (O-C2H4) 1-25-OH (such as nonoxynol-9), glycerol alkyl esters (such such as glyceryl laurate), polyoxyethylene glycol sorbitan alkyl esters (such as polysorbates, preferably polysorbate 20, polysorbate 40, polysorbate 60 , polysorbate 65, polysorbate 80, polysorbate 85 and / or polysorbate 120; these are all commercially available, for example, under the trademarks Canarcel® or Tween®), alkyl esters of sorbitan, cocamide MEA, cocamide DEA, dodecyldimethylamine oxide, block copolymers of polyethylene glycol and polypropylene glycol (such as poloxamers ( commercially available, for example, under the trademark Pluronic®)), and polyethoxylated tallow amine (POEA), - biotensoactives, preferably soforolipids, rhamnolipids and the mannosyl erythritol and lipopeptide lipids (the preferred lipopeptides are chlamydocine, surfactin, lichenisin G, and phenicine type lipopeptides).
[0416] [000416] Preferably, one, two, three, four, five or more fragrance substances with one or more notes selected from the group fresh, floral (flowery), aldehydes, watery, fruit, sweet, woody, musky, green and herbaceous, more preferably with one or more notes selected from the floral group (preferably rose and / or lily-of-the-valley (muguet)), aldehydes, vanilla, citrus, sandalwood, and musk.
[0417] [000417] The epidermis refers to the outermost layers of cells in the skin of a mammal, in particular certain parts of the body of a human, namely the hand, arm, foot, head and axillary region (particularly the axilla). The mucous membrane (mucosa) lines the cavities that are exposed to the external environment and the internal organs, particularly in the nostrils, the mouth (oral cavity), the lips, the eyelids, and the genital area.
[0418] - água, preferivelmente em uma quantidade total de 10% em peso ou mais, mais preferivelmente em uma quantidade total de 50% em peso ou mais, particular e preferivelmente em uma quantidade total de 60% em peso ou mais, - um ou mais álcoois selecionados a partir do grupo consistindo em etanol, 1,2-propileno glicol, glicerol (glicerina), 2-metil-1,3-propanodiol, 1,2-butanodiol, 1,3-butanodiol 1,4-butanodiol, 1,2,4-butanotriol, 1-pentanol, 1,2-pentanodiol, 1,5-pentanodiol, 1,2-hexanodiol, 1-octanol, 1,2-octanodiol, e 3-(2-etil-hexilóxi)propano-1,2-diol (etil-hexilglicerina, octoxiglicerina), preferivelmente em uma quantidade total de 1,5% em peso ou mais, mais preferivelmente em uma quantidade total de 2,5% em peso ou mais, em que as quantidades indicadas em cada caso referem-se ao peso total da composição desodorante cosmética. [000418] A cosmetic deodorant composition according to the present invention preferably comprises - an effective antimicrobial amount of one or more compounds of formula I and / or the physiologically acceptable salts thereof, preferably 2 ppm or more, more preferably 5 ppm or more , even more preferably 10 ppm or more, - water, preferably in a total amount of 10% by weight or more, more preferably in a total amount of 50% by weight or more, particularly and preferably in a total amount of 60% by weight or more, - one or more alcohols selected from the group consisting of ethanol, 1,2-propylene glycol, glycerol (glycerin), 2-methyl-1,3-propanediol, 1,2-butanediol, 1,3-butanediol 1,4 -butanediol, 1,2,4-butanotriol, 1-pentanol, 1,2-pentanediol, 1,5-pentanediol, 1,2-hexanediol, 1-octanol, 1,2-octanediol, and 3- (2-ethyl -hexyloxy) propane-1,2-diol (ethylhexylglycerin, octoxiglycerin), preferably in a total amount of 1.5% by weight or more, more preferably in a total amount of 2.5% by weight or more, in that the quantities indicated in each case refer to the total weight of the cosmetic deodorant composition.
[0419] [000419] Such a cosmetic deodorant composition according to the present invention is preferable and additionally comprises one or more constituents selected from the group consisting of additional antiperspirants, fragrance substances, and surfactants.
[0420] [000420] Antiperspirants inhibit the secretion of sweat. As antiperspirants, in general, astringent metal salts are used, in particular the inorganic and organic metal salts of the elements aluminum, zinc, magnesium, tin and zirconium, as well as mixtures thereof. Often aluminum and zirconium salts and mixtures thereof are also used in the form of complexes, with propylene glycol, polyethylene glycol or glycerin being used as complex forming agents. One or more antiperspirants are preferably selected from the group consisting of aluminum chlorohydrate; aluminum sesquichlorohydrate, aluminum chlorohydrex propylene glycol, aluminum dichlorohydrex propylene glycol, aluminum sesquichlorohydrex propylene glycol, aluminum chlorohydrex polyethylene glycol, aluminum dichlorohydrex polyethylene glycol, sesquichlorohydrexhydrex aluminum polyethylene glycol, aluminum chloride, aluminum zirconium chlorohydrate, aluminum zirconium trichlorohydrate, aluminum zirconium tetrachlorohydrate, aluminum zirconium pentachlorohydrate, aluminum zirconium octachlorohydrate, aluminum zirconium trichlorohydrate- glycerin, aluminum zirconium-glycerin tetrachlor-hydrex, aluminum zirconium-glycerin pentachloro-hydrex, aluminum zirconium-glycerin octachloride-hydroxide, basic aluminum chloride, zirconium hydroxide, zirconium chloride.
[0421] [000421] Preferably, one or more of the additional surfactants are selected from the group consisting of anionic surfactants, cationic surfactants, nonionic surfactants, or amphoteric surfactants, explicitly mentioned above.
[0422] - produtos à base de soja (preferivelmente leite de soja, bebidas de soja, iogurtes de soja), - bebidas e xaropes não alcoólicos (preferivelmente limonadas, concentrados de bebidas (xaropes), refrigerantes não gasosos, e refrigerantes gasosos), - bebidas alcoólicas, - produtos contendo 50% em peso ou mais de água e um ou mais outros extratos de ervas e/ou temperos (preferivelmente selecionados a partir do grupo consistindo em baunilha, canela, anis-verde, erva-doce, cravo, cardamomo, tamarindo, noz-moscada, pimenta-da-jamaica, pimenta-do-reino, alcaçuz, gengibre, fruto maduro da roseira, chá-verde, chá-vermelho, chá de rooibos, chá-mate, chá de honeybush, chá de pu-erh, chá oolong, chá-preto, grão de café, semente de cacau, hortelã-pimenta, hortelã, e gaultéria), - produtos de leite ou à base de leite, fermentados ou não fermentados, não congelados (preferivelmente leite, quark, requeijão, queijo, manjares, pudins, musses, bebidas à base de leite, iogurtes para beber, e iogurtes), - produtos congelados (preferivelmente sorvete, iogurte congelado, sorbet, leite gelado, manjares congelados, sorbets, sorvetes tipo granita, sorvete de frutas, e purês de frutas congelados), - massas de farinha e massas de farinha com ovos e leite (preferivelmente massa de farinha com ovos e leite para panquecas, massa de farinha para waffle, massas de farinha para bolos, massa de farinha para pães, massa de farinha para pãezinhos doces redondos, massa de farinha para massas), - emulsões de O/W (o que se passa sobre o pão, temperos, e molhos (para saladas). [000422] Examples of preferred materials according to the present invention, particularly orally consumable materials, are - products containing fruit or vegetables (preferably products containing juice, extract, puree, paste, pulp, concentrate, dried parts of lemon, lime, grapefruit, orange, lime orange, Seville orange, bergamot, tangerine, apple, pear, prickly pear, peach, apricot, fig, pineapple, prune, mango, melon, plum, kiwi, lychee, banana, cherry, wild cherry, strawberry, raspberry, red currant, black currant, blackberry, cranberry, marion blackberry, passion fruit, grapes (white grape, red grape, green grape, purple grape), pomegranate , acerola, tomato, carrot, parsnip, pumpkin, lettuce, cabbage, smoked cabbage, beans, peas, potatoes, peppers, red chilli, onion, celery, cucumber, leek, broccoli, cauliflower, radish, eggplant, zucchini ), - soy-based products (preferably soy milk, soy drinks, soy yoghurts), - non-alcoholic drinks and syrups (preferably lemonades, beverage concentrates (syrups), non-carbonated soft drinks, and carbonated soft drinks), - alcoholic beverages, - products containing 50% by weight or more of water and one or more other extracts of herbs and / or spices (preferably selected from the group consisting of vanilla, cinnamon, anise, fennel, cloves, cardamom, tamarind, nutmeg, allspice, black pepper, licorice, ginger, ripe rose hips, green tea, red tea, rooibos tea, mate tea, honeybush tea, pu-erh tea , oolong tea, black tea, coffee bean, cocoa bean, peppermint, mint, and wintergreen), - milk or milk-based products, fermented or non-fermented, not frozen (preferably milk, quark, curd, cheese, delicacies, puddings, mousses, milk-based drinks, drinking yogurts, and yoghurts), - frozen products (preferably ice cream, frozen yogurt, sorbet, frozen milk, frozen delicacies, sorbets, granita sorbets, fruit ice cream, and frozen fruit purees), - dough and dough with egg and milk (preferably dough with egg and milk for pancakes, dough for waffle, dough for cake, dough for bread, dough for round sweet rolls, dough for pasta), - O / W emulsions (what goes on in bread, spices, and dressings (for salads).
[0423] [000423] In a preferred embodiment, the one or more compounds of formula I and / or the physiologically acceptable salts thereof (for use) according to the present invention, the extracts according to the present invention are combined with one or more ingredients selected from the group consisting of lactic acid, lactose, sucrose, calcium salts (preferably calcium phosphate, calcium gluconate, calcium lactate, and calcium chloride), calcium oxide, magnesium salts, magnesium oxide, iron salts (preferably ferrous fumarate, ferrous succinate, iron sucrate-malate, iron fructate-malate, iron sucrate-citrate, iron fructatocitrate, iron sucrate-ascorbate, iron fructate-ascorbate, and mixtures thereof) , vitamin A (particularly retinol (vitamin A1)), vitamin B6, vitamin B12, vitamin C, vitamin D, vitamin E, thiamine, niacin, biotin, riboflavin, pantothenic acid, phytic acid, daidzein, genistein, proteins (preferably ine, caseinates (preferably sodium caseinate), milk protein, milk protein hydrolyzate, milk protein isolate, whey protein, whey protein hydrolyzate, whey protein isolate, milk protein soy, soy protein hydrolyzate, soy protein isolate), milk powder, soy powder, polyunsaturated fatty acids [referably omega-3-, omega-6- and / or omega-9-fatty acids, preferably selected from the group consisting of docosahexaenoic acid (DHA, all-c / s-docosa-4,7,10,13,16,19-hexaenoic acid), eicosatetraenoic acid (ETA, all-c / 's-8 acid , 11,14,17-eicosatetraenoic acid), eicosatetraenoic acid (ETA, all-cis-8,11,14,17-eicosatetraenoic acid), stearidonic acid (SDA, all-cis-6,9,12,15-octadecatetraenoic acid ), docosapentaenoic acid (DPA; clupanodonic acid, all-cis-7,10,13,16,19-docosapentaenoic acid), linoleic acid, α-linolenic acid (all-cis-9,12,15-octadecatrienoic acid), and γ-linolenic acid], soybean oil, cream, fish oil (refined), algae oil, squid oil, flaxseed oil, grape residue oil, and triglycerides derived from fatty acids myristic acid, palmitic acid and / or oleic acid, thereby forming preferred materials according to the present invention, particularly materials suitable for oral consumption.
[0424] [000424] Preferably, a material according to the present invention (preferably comprising a preferred or particularly preferred total amount, defined above, of water and having a pH value in a preferred or particularly preferred range indicated above), suitable for the oral consumption, comprises a total amount of glutamic acid and sodium glutamate of less than 0.2% by weight, preferably less than 0.15% by weight, more preferably less than 0.1% by weight, particularly and preferably less than 0.05% by weight, and most preferably it is still free of glutamic acid and sodium glutamate.
[0425] [000425] Examples of stabilizers and / or thickeners which can be part of a material (preferably orally consumable) according to the present invention are preferably selected from the group consisting of carbohydrate polymers (polysaccharides, preferably starches, polydextrose (number E E1200), physically modified starches, chemically modified starches (preferably oxidized starch (number E E1404), mono starch phosphate (number E E1410), diamide phosphate (number E E1412), phosphate diamide phosphate (number E E1413) , acetylated diamide phosphate (number E E1414), acetylated starch (starch acetate esterified with acetic anhydride; number E E1420), acetylated diamide adipate (number E E1422), propyl hydroxyl starch (number E E1440), propyl hydroxy phosphate diamido (number E E1442) starch octenyl succinate sodium (number E E1450), and oxidized acetylated starch (number E E1451)), cyclodextrins, celluloses, modified celluloses (preferably me tilcellulose, ethylcellulose, hydroxymethyl cellulose, hydroxyethyl cellulose, hydroxypropyl cellulose, hydroxyethyl methylcellulose, hydroxypropyl methylcellulose), gum arabic (acacia gum), ghatti gum, tragacanth gum, caraway gum, carrageenan, guar gum, locust bean gum (fine flour) locust bean gum, number E E410), alginates, pectins, inulin and xanthan gum.
[0426] [000426] In an additional preferred embodiment, the total amount of compounds of formula I and the physiologically acceptable salts thereof in an orally consumable material according to the present invention, in particular a ready-to-drink composition according to the present invention , is in the range of 1 to 200 ppm, more preferably in the range of 2 to 150 ppm, even more preferably in the range of 5 to 100 ppm, in each case based on the total weight of the orally consumable material.
[0427] [000427] Preferably, the total amount of glucose, fructose and sucrose of an orally consumable material according to the present invention, in particular a ready-to-drink composition according to the present invention, is in the range of 1.5 to 15.0% by weight, more preferably in the range of 2.0 to 12.0% by weight, even more preferably in the range of 2.5 to 10.5% by weight, particularly and preferably in the range of 3.0 to 9.5% by weight, in each case based on the total weight of the material orally consumable.
[0428] [000428] Glucose, fructose and sucrose are commercially and readily available from several sources and in different forms, and can be obtained from suitable plant sources, for example, from beet (Beta vulgaris ssp. , sugar fractions, sugar syrup, molasses), from sugar cane (Saccharum officinarum ssp., for example, molasses, sugar syrups), from maple (Acer ssp.), from agave (thick agave juice), sorghum, certain palm trees, inverted sugar syrup, high fructose corn syrup (HFCS, also called glucose-fructose syrup, for example, prepared from wheat or corn starch), or fruit concentrates (for example, from apples or pears, apple syrup, pear syrup).
[0429] [000429] In a preferred embodiment, an orally consumable material according to the present invention, in particular a ready-to-drink composition according to the present invention, preferably comprises one, two, three or more fruit flavoring agents having a molecular weight in the range of 135 to 190 g / mol, more preferably a molecular weight in the range of 135 to 180 g / mol, said fruit flavoring agents preferably imparting a note of flavor selected from the group consisting of lemon, lime, grapefruit, orange, lime orange, Seville orange, bergamot, mandarin, apple, pear, cloves, peach, apricot, pineapple, prune, mango, melon, plum, kiwi, lychee, banana, cherry, wild cherry, strawberry, raspberry, red currant, black currant, blackberry, cranberry, passion fruit, grape, pomegranate, acerola, coconut, vanilla and mixtures thereof.
[0430] [000430] Preferably, an orally consumable material according to the present invention, in particular a ready-to-drink composition according to the present invention, comprises one or more organic food acids (i.e., organic acids suitable for oral consumption ), preferably selected from the group consisting of acetic acid, adipic acid, caffeic acid, citric acid, isocitric acid, maleic acid, fumaric acid, galacturonic acid, glycuronic acid, glycolic acid, glycolic acid, lactic acid, malic acid, oxalic, pyruvic acid, quinic acid, succinic acid, tannic acid, tartaric acid, and their physiologically acceptable salts, preferably the sodium and / or potassium and / or calcium and / or magnesium salts thereof.
[0431] [000431] Preferred physiologically acceptable salts of phosphoric acid are, for example, sodium acetate, monosodium phosphate, disodium phosphate, monopotassium phosphate, dipotassium phosphate, sodium hexametaphosphate, and bisphosphonates of sodium.
[0432] [000432] Preferably, an orally consumable material according to the present invention, in particular a ready-to-drink composition according to the present invention, comprises one or more acids selected from the group consisting of citric acid, tartaric acid, acid lactic acid, malic acid, maleic acid, fumaric acid, phosphoric acid, pyrophosphoric acids, polyphosphoric acids, bisphosphonic acids and their physiologically acceptable salts.
[0433] - sacarose, e/ou - uma mistura de glicose e frutose, em que a quantidade de frutose está na faixa de 30 a 95% em peso, preferivelmente 40 a 92% em peso, com base na quantidade total de glicose e frutose no material oralmente consumível. [000433] A more preferred orally consumable material according to the present invention, in particular a ready-to-drink composition according to the present invention, comprises - sucrose, and / or - a mixture of glucose and fructose, in which the amount of fructose is in the range of 30 to 95% by weight, preferably 40 to 92% by weight, based on the total amount of glucose and fructose in the orally consumable material.
[0434] - um ou mais emulsificantes, e/ou - um ou mais antioxidantes e opcionalmente uma ou mais substâncias para intensificar o efeito antioxidante dos ditos antioxidantes, e/ou - um ou mais conservantes, e/ou - uma ou mais vitaminas e os sais ou ésteres fisiologicamente aceitáveis das mesmas, e/ou - um ou mais agentes corantes, e/ou - um ou mais agentes pesantes, e/ou - um ou mais álcoois de açúcares, e/ou - um ou mais adoçantes de alta potência, preferivelmente um ou mais adoçantes de alta potência que ocorrem naturalmente, e/ou - um ou mais estabilizantes e/ou espessantes. [000434] Another preferred orally consumable material according to the present invention, in particular a ready-to-drink composition according to the present invention, comprises one or more additional constituents, suitable for consumption, selected from: - one or more emulsifiers, and / or - one or more antioxidants and optionally one or more substances to enhance the antioxidant effect of said antioxidants, and / or - one or more preservatives, and / or - one or more vitamins and their physiologically acceptable salts or esters, and / or - one or more coloring agents, and / or - one or more weighing agents, and / or - one or more sugar alcohols, and / or - one or more high potency sweeteners, preferably one or more naturally occurring high potency sweeteners, and / or - one or more stabilizers and / or thickeners.
[0435] - um ou mais emulsificantes, preferivelmente selecionados a partir do grupo consistindo em lecitinas (preferivelmente lecitinas que ocorrem naturalmente, particularmente a lecitina de ovo ou soja), fosfolipídios (preferivelmente fosfatidilcolinas), monoacilgliceróis, e diacilgliceróis, e/ou - um ou mais antioxidantes e opcionalmente uma ou mais substâncias para intensificar o efeito antioxidante dos ditos antioxidantes, e/ou - um ou mais conservantes (preferivelmente selecionados a partir do grupo consistindo em ácido benzoico, benzoato de sódio, benzoato de potássio, ácido sórbico, sorbato de sódio, sorbato de sódio, hidroxianisol butilado (BHA), e hidroxitolueno butilado (BHT)), preferivelmente em uma quantidade total de 0,05 a 0,5% em peso, mais preferivelmente de 0,1 a 0,3% em peso, com base no peso total da composição, e/ou - uma ou mais vitaminas e os sais ou ésteres fisiologicamente aceitáveis das mesmas, preferivelmente selecionados a partir do grupo consistindo em vitamina A, palmitato de vitamina A, vitamina B1, vitamina B2 (riboflavina), vitamina B3 (niacina), vitamina B6, vitamina B9 (ácido fólico) vitamina B12, vitamina C (ácido ascórbico), ascorbato de monossódio, ascorbato de monopotássio, diascorbato de cálcio, diascorbato de magnésio, palmitato de ascorbila, estearato de ascorbila, vitamina D, e vitamina E, acetato de vitamina E, palmitato de vitamina E, vitamina H (biotina), vitamina K, e/ou - um ou mais agentes corantes, preferivelmente selecionados a partir do grupo consistindo em carotenos (Número E E160a, preferivelmente beta-caroteno), extrato de páprica (Número E E160c), pó do suco da beterraba vermelha (compreendendo betanina, vermelho beterraba, Número E E162), urucu (Número E E160b), antocianinas (Número E E163), clorofilas (Número E E140), curcuma (Número E E100, compreendendo curcumina), tartrazina (Amarelo FD&C No. 5, Número E E102), amaranto (Número E E123), dióxido de titânio (Número E E171), óxidos de ferro e hidróxidos de ferro (Número E E172), eritrosina (Número E E127), cor de caramelo (Número E E150, preferivelmente E150d), amarelo FD&C No. 6 (Número E E110), vermelho allura (vermelho FD&C No.40, Número E E129), verde FD&C No. 3 (verde firme, Número E E143), azul FD&C No. 1 (azul brilhante, Número E E133) e azul FD&C No. 2 (indigotina, Número E E132), e/ou - uma ou mais substâncias que tenham um sabor amargo selecionadas a partir do grupo consistindo em quinina, neo-hesperidina, hesperidina, naringina, quercitrina, floridzina, floretina-2-O'-xiloglicosídeo, ácido caféico, ácido clorogênico, ácido neoclorogênico, ácido criptoclorogênico, limonóides (preferivelmente limonina ou nomilina a partir de frutas cítricas), lupolonas a partir de lúpulos, humulonas a partir de lúpulos, ésteres de ácidos gálico e elágico de carboidratos (preferivelmente pentagaloilglicose), catequinas e epicatequinas (preferivelmente selecionadas a partir do grupo consistindo em catequinas galoiladas, epicatequinas galoiladas, galocatequinas ou epigalocatequinas, galocatequinas galoiladas ou epigalocatequinas galoiladas), teaflavinas (em particular teaflavina, isoteaflavina, neoteaflavina), teaflavinas galoiladas, e procianidinas (= proantocianidinas) (em particular Procianidina B1, Procianidina B2, Procianidina A2, Procianidina B5, e Procianidina C1), e/ou - um ou mais estabilizantes e/ou espessantes, preferivelmente selecionados a partir do grupo consistindo em octenil succinato de sódio, carboximetil celulose, maltodextrina, goma Arábica, goma guar, goma de alfarroba, alginatos, pectina, e goma xantana. [000435] Preferably, an orally consumable material according to the present invention, in particular a ready-to-drink composition according to the present invention, comprises one or more additional constituents, suitable for oral consumption, particularly - one or more emulsifiers, preferably selected from the group consisting of lecithins (preferably naturally occurring lecithins, particularly egg or soy lecithin), phospholipids (preferably phosphatidylcholines), monoacylglycerols, and diacylglycerols, and / or - one or more antioxidants and optionally one or more substances to enhance the antioxidant effect of said antioxidants, and / or - one or more preservatives (preferably selected from the group consisting of benzoic acid, sodium benzoate, potassium benzoate, sorbic acid, sodium sorbate, sodium sorbate, butylated hydroxyanisole (BHA), and butylated hydroxytoluene (BHT)), preferably in a total amount of 0.05 to 0.5% by weight, more preferably from 0.1 to 0.3% by weight, based on the total weight of the composition, and / or - one or more vitamins and their physiologically acceptable salts or esters, preferably selected from the group consisting of vitamin A, vitamin A palmitate, vitamin B1, vitamin B2 (riboflavin), vitamin B3 (niacin), vitamin B6, vitamin B9 (folic acid) vitamin B12, vitamin C (ascorbic acid), monosodium ascorbate, monopotassium ascorbate, calcium diascorbate, magnesium diascorbate, ascorbyl palmitate, ascorbyl stearate, vitamin D, and vitamin E, vitamin E acetate , vitamin E palmitate, vitamin H (biotin), vitamin K, and / or - one or more coloring agents, preferably selected from the group consisting of carotenes (Number E E160a, preferably beta-carotene), paprika extract (Number E E160c), red beet juice powder (comprising betanine, red beet, number E E162), annatto (Number E E160b), anthocyanins (Number E E163), chlorophylls (Number E E140), turmeric (Number E E100, comprising curcumin), tartrazine (Yellow FD&C No. 5, Number E E102), amaranth ( Number E E123), titanium dioxide (Number E E171), iron oxides and iron hydroxides (Number E E172), erythrosine (Number E E127), caramel color (Number E E150, preferably E150d), yellow FD&C No. 6 (Number E E110), allura red (red FD&C No.40, Number E E129), green FD&C No. 3 (solid green, Number E E143), blue FD&C No. 1 (bright blue, Number E E133) and blue FD&C No. 2 (indigotine, Number E E132), and / or - one or more substances that have a bitter taste selected from the group consisting of quinine, neo-hesperidin, hesperidin, naringin, quercitrin, floridzine, floretine-2-O'-xyloglycoside, caffeic acid, chlorogenic acid, neochlorogenic acid, acid cryptochlorogenic, limonoids (preferably limonine or nomilin from citrus fruits), lupolones from hops, humulones from hops, esters of gallic and ellagic carbohydrates (preferably pentagalloyl glucose), catechins and epicatechins (preferably selected from the group consisting of galloated catechins, galloated epicatechins, galocatechins or epigallocatechins, galloated galocatechins or galloated epigallocatechins), teaflavins (in particular teaflavine, isoteaflavine, neoteaflavine), galloated teaflavines, and procyanidins, Procyanidins (Procanidines, Procyanidins, Procyanidins, Procyanidins, Procyanidins, Procyanidins, , Procyanidin B5, and Procyanidin C1), and / or - one or more stabilizers and / or thickeners, preferably selected from the group consisting of sodium octenyl succinate, carboxymethyl cellulose, maltodextrin, gum arabic, guar gum, locust bean gum, alginates, pectin, and xanthan gum.
[0436] [000436] Examples of stabilizers and / or thickeners that can be part of an orally consumable material according to the present invention, in particular a ready-to-drink composition according to the invention, are preferably selected from the group consisting of carbohydrate polymers (polysaccharides), cyclodextrins, starches, degraded starches (starch hydrolysates), chemically or physically modified starches (preferably sodium octenyl succinate starch, E1450), modified celluloses (preferably carboxymethyl cellulose), gum arabic (acacia gum) , ghatti gum, tragacanth gum, caraia gum, carrageenan, guar gum, locust bean gum (fine locust bean flour), alginates, pectin, inulin and xanthan gum.
[0437] [000437] If an orally consumable material according to the present invention, in particular a ready-to-drink composition according to the present invention, comprises one or more thickeners, then the total amount of thickeners is preferably in the range of 0.0025 to 1% by weight, more preferably in the range of 0.01 to 0.4%, even more preferably in the range of 0.015 to 0.2%, in each case based on the total weight of the composition.
[0438] [000438] An orally consumable material according to the present invention, in particular a ready-to-drink composition according to the present invention, preferably comprises 200 ppm or more of hydrogen carbonate (HCO3-), more preferably 250 ppm or more of hydrogen carbonate, even more preferably 300 ppm or more of hydrogen carbonate, and particularly and preferably 400 ppm or more of hydrogen carbonate, in each case based on the total weight of the orally consumable material.
[0439] [000439] If an orally consumable material according to the present invention, in particular a ready-to-drink composition according to the present invention, is gaseous, the total amount of carbon dioxide (CO2) is preferably in the range of 0.02 to 5.0% by weight, more preferably in the range of 0.05 to 3% by weight, even more preferably in the range of 0.1 to 2.5% by weight, particularly and preferably in the range of 0.2 to 2 , 0% by weight, more preferably still in the range of 0.25 to 1.5% by weight, in each case based on the total weight of the orally consumable material.
[0440] [000440] An orally consumable material according to the present invention, in particular a ready-to-drink composition according to the present invention, may additionally comprise lactose and / or maltose, and / or one or more sugar alcohols, such as such as dulicitol, fucitol, maltitol, erythritol, isomaltitol (E 953), lactitol (E 966), maltitol, mannitol (E421), sorbitol (E420), xylitol (E967), and mixtures thereof.
[0441] [000441] An orally consumable material according to the present invention, in particular a ready-to-drink composition according to the present invention, may additionally comprise one or more high potency sweeteners which are preferably selected from the group consisting of cyclamate of sodium, acesulfame K, neo-hesperidin dihydrocalcone, saccharin, sodium saccharin salt, aspartame, superaspartame, neotame, alitame, sucralose, magap, lugduname, carrelame, sucrononate, sucro-octate, miraculin, curculine, monelin, mabinline, taatin curculine, brazein, pentadine, or their extracts or fractions obtained from natural sources containing said amino acids and / or proteins, neo-hesperidin dihydrocalcone, steviolglycoside, stevioside, steviolbioside, rebaudiosides (preferably reaudioside A, reaudioside B, rebaudioside C, rebaudioside C, , rebaudioside D, rebaudioside E, rebaudioside F, rebaudioside G, rebaudioside H, dulcoside, rubusoside), suaveioside A, smoothioside B, smoothioside G, smoothioside H, smoothioside I, smoothioside J, baiyunoside 1, baiyunoside 2, flomisoside 1, flomisoside 2, flomisoside 3, flomisoside 4, abrusoside A, abrusoside B, abrusoside C, abrusoids D, cyclocariosides A and cyclocarioside I, oslandin, polypodoside A, estrogin 1, estrogin 2, estrogin 4, seligueanin A, dihydroquercetin-3-acetate, perilartine, telosmoside A15, periandrine IV, pterocarioside, cyclocarioside, mucurozioside, brioside, brionidside, brionid, brionid scandenoside, gipenoside, trilobatin, floridzine, dihydroflavanol, hematoxylin, cyanine, chlorogenic acid, albiziasaponine, telosmoside, gaudicaudioside, mogroside, hernandulcin, monatin, glycyrrhetin, glycyrrhizin, the same ones, the same ones, the same ones, the same ones, the same ones potassium, sodium, calcium or ammonium salts, liquorice extracts (Glycyrrhizza glabra ssp.), extracts of Lippia dulcis, extra Momordica ssp. or individual substances (in particular Momordica grosvenori [Luo Han Guo] and the mogrosides obtained from it), extracts of Hydrangea dulcis or Stevia ssp. (e.g. Stevia rebaudiana) or individual substances.
[0442] [000442] An orally consumable material according to the present invention, in particular a ready-to-drink composition according to the present invention, preferably comprises one or more high potency sweeteners, preferably selected from the group indicated above, more preferably selected from the group consisting of aspartame, neotame, superaspartame, advantame, saccharin, sucralose, cyclamate, acesulfame, tagatose, moneline, stevioside, rebaudioside A, rebaudioside C, rebaudioside D, rubusoside, fylodulcine, hernandulcin, tamarine, glycine , glycyrrhetinic acid, the physiologically acceptable salts (preferably the sodium, potassium or calcium salts) of these compounds.
[0443] [000443] An orally consumable material according to the present invention, in particular a ready-to-drink composition according to the present invention, preferably comprises a total amount of less than 4.5% by weight of fats and fatty oils (i.e. is triglycerides), more preferably less than 3.5% by weight of fats and fatty oils, even more preferably less than 2.0% by weight of fats and fatty oils, particularly and preferably less than 1.0% by weight. weight of fat and fatty oils, and more preferably still less than 0.5% by weight of fatty and fatty oils, in each case based on the total weight of the orally consumable material.
[0444] [000444] An orally consumable material according to the present invention, in particular a ready-to-drink composition according to the present invention, preferably comprises a total amount of less than 5.0% by weight of proteins, more preferably less than than 4.0% by weight of proteins, even more preferably less than 3.0% by weight of proteins, particularly and preferably less than 1.0% by weight of proteins, and more preferably still less than 0.5 % by weight of proteins, in each case based on the total weight of the material orally consumable.
[0445] [000445] Preferred orally consumable materials according to the present invention are alcoholic or non-alcoholic drinks (preferably drinks containing coffee, drinks containing tea, drinks containing cocoa, drinks containing wine, drinks containing beer, soft drinks containing fruit, isotonic drinks, soft drinks , energy drinks, nectars, fruit and vegetable juices, instant beverage powders after dilution in water, beverage concentrates, beverage syrups, fountain syrups, milk shakes), milk products (preferably flavored milk, yoghurts, yoghurts, sour milk drink, semi-skim milk drinks, milk shakes, milk mix drinks), ice products (sorbet, ice cream), fruit preparations (preferably sorbets, fruit jams, fruit fillings, fruit ice creams ), vegetable products (preferably soy milk products, ketchup, sauces), emulsions (preferably mayonnaise, remolada, condiments, in bakery essences), fruit jellies, fruit preserves, bakery fillings, pickle brine, frozen juice compositions, sour candies, fruit pie fillings, desserts, wine vines, and soups.
[0446] [000446] A beverage (preferably acidic) of this invention can be prepared, for example, from a corresponding syrup composition (preferably acidic) based on a dilution or discharge of the syrup (preferably acidic). Those skilled in the art recognize that a common discharge for a soda, for example, cola-flavored carbonated soda, is 1 + 5, so that a preparer uses one part of the cola syrup and five parts of water to prepare the drink (preferably acid) from the syrup (preferably acid). The amount of syrup (preferably acidic) used to prepare the drink (preferably acidic) of this invention will, of course, vary depending on the concentration of the syrup and the desired end product. Such an amount can be readily determined by those of ordinary skill in the art.
[0447] [000447] In a preferred embodiment, an orally consumable material according to the present invention, in particular a ready-to-drink composition according to the present invention, is transparent. The term "transparent", in the context of the present invention, refers to a composition of matter having a turbidity of less than 25 FNU (Formazina Nephelometric Units), as measured according to DIN EN ISO 7027 - Water quality - Determination of turbidity (ISO 7027: 1999).
[0448] [000448] Preferably, an orally consumable material according to the present invention, in particular a ready-to-drink composition according to the present invention, has a turbidity of less than 12 FNU, more preferably less than 6 FNU, preferably measured with a Hach 2100N IS turbidimeter.
[0449] [000449] The preferred orally consumable materials according to the present invention are, in particular, transparent or cloudy (aerated or non-aerated) drinks, preferably selected from the group consisting of lemonade, aerated soft drinks, tea, iced tea, mixtures of beer-lemonade, coca-cola, beer-cola mixes, whey drink lemonade, tea, beer-lemonade mixes, cola drinks, beer-cola mixes, and whey drinks, and concentrates for produce said drinks.
[0450] [000450] An orally consumable material according to the present invention (as defined above), at 20 ° C and 101.3 kPa (1013 mbar), is preferably capable of flowing, and more preferably is liquid.
[0451] [000451] An orally consumable material according to the present invention, in particular a ready-to-drink composition according to the invention, preferably has a dynamic viscosity value of less than 1250 mPa s (mPa s = milli Pascal seconds; equal a cP = centiPoise), preferably less than 600 mPa s, more preferably less than 250 mPa s, particularly and preferably less than 100 mPa s, especially and preferably less than 50 mPa s, and more preferably still less than 25 mPa s, in each case measured at 20 ° C and at a shear rate of D = 10 s-1, for example, as determined with a Brookfield® viscometer according to DIN 53018.
[0452] [000452] An orally consumable material according to the present invention, in particular a ready-to-drink composition according to the invention, preferably has a dynamic viscosity value in the range of 1 to 1000 mPa s (mPa s = milli Pascal seconds; equal to cP = centiPoise), preferably in the range of 2 to 500 mPa s, more preferably in the range of 2 to 125 mPa s, particularly and preferably in the range of 3 to 50 mPa s, especially and preferably in the range of 3 to 25 mPa s, in each case measured at 20 ° C according to DIN 53018.
[0453] [000453] The taste of an orally consumable material according to the present invention, in particular a ready-to-drink composition according to the present invention, is preferably selected from the group consisting of berries, citrus fruits, pomegranate fruits, condiments , herbs, mint, tea, coffee, milk and / or milk products, and more particularly and preferably selected from the group consisting of cola, lemon, lime, lemon and lime soda, grapefruit, orange, orange lemon, Seville orange, bergamot, mandarin, apple, pear, cloves, peach, apricot, pineapple, prune, mango, melon, plum, kiwi, lychee, banana, cherry, wild cherry, strawberry, raspberry, redcurrant, blackcurrant, blackberry, cranberry, passion fruit, grape, pomegranate, acerola, vanilla, cinnamon, anise, fennel, cloves, cardamom, tamarind, nutmeg, allspice, black pepper, honey, licorice, ginger soda, ginger, beer soda root, ripe rose hips, green tea, red tea, rooibos tea, mate tea, honeybush tea, pu-erh tea, oolong tea, black tea, kombucha, milk, coffee, espresso, cocoa , chocolate, hazelnut, walnut, almond, peppermint, mint, wintergreen and mixtures thereof.
[0454] [000454] In a preferred embodiment, an orally consumable material according to the present invention, in particular a ready-to-drink composition according to the present invention, comprises one or more amino carboxylic acids and / or one or more sulfonic amino acids, preferably gamma-amino butyric acid and / or taurine (2-aminoethanesulfonic acid).
[0455] [000455] An orally consumable material according to the present invention, in particular a ready-to-drink composition according to the present invention, can preferably comprise one or more ingredients derived from fruits, in particular fruit essences, fruit juices, purees of fruit and fruit juice concentrates.
[0456] [000456] Fruit juices or fruit juice concentrates that can be used are preferably derived from citrus fruits, such as orange, lemon, grapefruit and tangerine, and other fruits, such as apple, pear , grape, apricot and pineapple. In addition, fruit juices and fruit juice concentrates can be derived from soft fruits, such as blackberry, gooseberry, gooseberry, cranberry, cob, strawberry and raspberry.
[0457] [000457] Preferably, an orally consumable material according to the present invention, in particular a ready-to-drink composition according to the present invention, is an emulsion. The densities of the dispersed phase, which are preferred for proper stabilization and avoiding ring formation, preferably are in the range of 0.92 to 1.06 g / ml, more preferably in the range of 0.94 to 1.03 g / ml. "Ring formation" is the formation of a ring around the neck of a (drink) container, which is sought to be avoided.
[0458] [000458] If an orally consumable material according to the present invention, in particular a ready-to-drink composition according to the present invention, comprises one or more weighing agents, these are preferably selected from the group consisting of sucrose isobutyrate acetate (SAIB, E 444), ester gum (E 445), gum-damar, and brominated vegetable oils, in an amount that does not exceed the respective legally authorized concentrations.
[0459] [000459] In a preferred embodiment, an orally consumable material according to the present invention, in particular a ready-to-drink composition according to the present invention, is a cloudy (turbid) emulsion, preferably comprising one or more turbid agents, such as titanium dioxide, palm oil, or terpene oils, such as limonene.
[0460] [000460] If an orally consumable material according to the present invention, in particular a ready-to-drink composition according to the present invention, is an emulsion, for example, prepared as described in US 5,616,358, in EP 2 025 250 or EP 1 151 677.
[0461] [000461] If an orally consumable material according to the present invention, in particular a ready-to-drink composition according to the present invention, is a cloudy (turbid) emulsion, the particle size (droplet) D90 of the dispersed phase (as measured by laser diffraction) is in the range of 0.15 to 1.0 μm (micron), in some preferred modalities in the range of 0.35 to 0.5 μm, in some other preferred modalities in the range of 0.6 to 0.75 μm.
[0462] [000462] In a preferred embodiment, a beverage according to the present invention comprises a terpene oil, more preferably a citrus terpene oil. The terpene oils preferred in the context of the present invention comprise or consist of orange, lemon and / or grapefruit oils and their fractions, preferably limonene (especially D-limonene) and / or orange oil terpenes.
[0463] [000463] A material according to the present invention, preferably a food or cosmetic product according to the present invention, may additionally comprise one or more physiological refrigerants, preferably selected from the group consisting of menthol derivatives (preferably L- menthol glycerol ketal), p-menthane-3,8-diol, baseball, isopulegol and esters thereof (preferably L - (-) - isopulegol, L - (-) - isopulegol acetate), mentholic ethers (preferably (L- menthoxy) -1,2-propanediol, (L-menthoxy) -2-methyl-1,2-propanediol, L-menthyl-methyl ether), menthol esters (preferably menthol format, menthol acetate, menthol isobutyrate, menthol lactate, L-mentyl-L-lactate, L-mentyl-D-lactate, L-mentyl- (2-methoxy) acetate, L-mentyl- (2-methoxyethoxy) acetate, L-menthyl pyroglutamate, carbonates menthol (preferably L-menthyl propylene glycol carbonate, L-menthyl ethylene glycol carbonate, L-mentyl glycerol carbonate a or mixtures thereof), semesters of mentholes with a dicarboxylic acid or its derivatives (preferably menthyl oxamate, menthyl-N-methyloxamate, menthyl-N-ethyloxamate, mono-L-menthyl succinate, mono-L-menthyl glutarate , mono-L-menthyl malonate, N-menthyl succinic acid ester-N, N- (dimethyl) amide, OL-menthyl succinic acid ester amide), 2,3-dimethyl-2- (2- propyl) -butanoic acid (preferably 2,3-dimethyl-2- (2-propyl) -butanoic acid-N-methyl amide [WS-23]), amine carboxylic acid amides (preferably L-mentane carboxylic acid-N-ethyl amide [WS-3], Na- (L-mentanocarbonyl) glycine ethyl ester [WS-5], M-carboxylic acid-N- (4-methoxyphenyl) -amide [WS-12], L-menthane carboxylic acid-N- tert-butyl amide [WS-14], L-menthane carboxylic acid-N- (4-cyanophenyl) amide, N- (4-cyanomethylphenyl) p-mentanecarboxamide), L-menthane carboxylic acid-N- (alkoxyalkyl) amides, L-mentane carboxylic acid-N- (alkylthioalkyl) amides, and pyrrolidone derivatives of cycloalkyldione derivatives (preferably 3-methyl-2 (1-pyrrolidinyl) -2-cyclopenten-1-one).
[0464] [000464] In one aspect of the present invention, the preferred cosmetic products of the invention are oral care products (oral care products). The preferred oral care products are creams, gels, pastes, foams, emulsions, suspensions, aerosols, sprays or chewing gums. Such formulations are used to clean and care for the substance of the tooth and oral cavity and to freshen the breath. The most preferred oral care products are toothpastes, tooth gels, 2 in 1 tooth gels, mouthwashes, mouthwashes, gargle fluids and mouth or breath sprays.
[0465] [000465] A cosmetic product, in particular for oral hygiene according to the invention, may contain additional auxiliary substances, such as are conventionally used in such preparations, for example, additional preservatives, abrasives, antibacterial agents, anti-inflammatory agents, irritation-preventing agents, irritation-inhibiting agents, additional antimicrobial agents, antioxidants, astringents, antiseptic, antistatic, binding agents, buffers, support materials, chelating agents, cell stimulants, cleansing agents, conditioning agents, additional surfactants, deodorants, softeners, emulsifiers, enzymes, essential oils, film-forming agents, foaming agents, foam stabilizers, substances to prevent the formation of foams, gelling agents, moisturizing substances, substances that retain moisture, whitening agents, optical brighteners, agents that repel dirt, lubricate dyes, opacifying agents, illuminators, polymers, powders, proteins, silicones, agents that soothe the skin, agents that cleanse the skin, agents for skin care, agents that heal the skin, agents that refresh the skin, agents that heat the skin, stabilizers, thickeners, vitamins, oils, waxes, fats, phospholipids, saturated fatty acids, mono- or polyunsaturated fatty acids, alpha-hydroxy acids, polyhydroxy fatty acids, dyes, color-protecting agents, pigments , flavoring substances, perfumes, and other conventional constituents, such as alcohols, polyols, electrolytes, organic solvents, sweeteners, sugar substitutes, silicas, calcium carbonate, calcium hydrogen phosphate, aluminum oxide, fluorides, zinc salts, tin, potassium, sodium and strontium, pyrophosphates, hydrogen peroxide, and hydroxyapatites.
[0466] [000466] Examples of flavoring substances which may preferably be part of an oral care product according to the invention are: anethole, menthol, menthol, isomentone, menthyl acetate, menthol propionate, mentofuran, mint lactone, eucalyptol (1,8-cineol), limonene, eugenol, eugenol acetate, methyl isoeugenol ether, thymol, pinene, sabinene hydrate, 3-octanol, carvone, gamma-octalactone, gamma-nonalactone, germacrene-D, viridiflorol, 1, 3E, 5Z-undecatriene, isopulegol, piperitone, 2-butanone, ethyl formate, 3-octyl acetate, isoamyl isovalerianate, hexanol, hexanal, cis-3-hexenol, linalool, alpha-terpineol, cis- and trans acetate -carvila, p-cimol, damascenone, damask, rose oxide, fenchol, diethyl acetaldehyde acetate, 1-ethoxyethyl acetate, cis-4-heptenal, isobutyraldehyde, isovaleraldehyde, cis-jasmine, methyl dihydrojasmonate, anisaldehyde, salic methyl, 2'-hydroxypropiophenone, menthyl methyl ether, mirtenyl acetate, 2-phenylethyl alcohol single, 2-phenylethyl isobutyrate, 2-phenylethyl isovalerate, cinnamaldehyde, geraniol, nerol. In the case of chiral compounds, aroma substances can be used as a single enantiomer or a mixture of enantiomers, for example, in the form of a racemate.
[0467] [000467] The flavor substances which are preferably part of an oral hygiene product according to the invention are preferably selected from the group consisting of anise seed oil, basil oil, sour almond oil, camphor oil, citronella oil, citrus fruit oils, eucalyptus citriodora oil, eucalyptus oil, chamomile oil, mint oil, lime oil, tangerine oil, clove oil, orange oil , peppermint oil, sage oil, thyme oil, oil of wintergreen, cinnamon oil, cinnamon bark oil, I-menthol, menthol, isomentone, 1,8-cineol (eucalyptol), carvone, alpha- terpineol, methyl salicylate, 2'-hydroxypropiophenone, and methyl menthol.
[0468] [000468] Another group of preferred cosmetic products of the invention are sanitary articles, preferably selected from the group consisting of wet towels, sanitary napkins, diapers, vaginal tampons, tissues and refreshing tissues containing one or more compounds of formula I (to be used) according to the invention and / or one or more physiologically acceptable salts thereof. Said sanitary articles according to the invention preferably contain a material according to the present invention, in particular in one of the preferred variants described in this document.
[0469] [000469] In a non-woven system, preferably at least one layer comprises an absorbent non-woven fabric or a porous polymer, which is impregnated with a solution or suspension comprising one or more compounds of formula I (to be used) according to the invention and / or one or more physiologically acceptable salts thereof, and preferably comprises one or more other additional active substances (such as skin-smoothing and / or skin-hydrating agents).
[0470] 1) SDB (Caldo de Dextrose Sabouraud, Ref. 238230, Difco® Lawrence, KS, EUA, contendo 0,5% de Digesto Peptídico de Tecido Animal, 0,5% de Digesto Pancreático de Caseína, 2,0% de Dextrose, pH 5,6. 2) Meio YMG (Levedura-Malte-Glicose): D-glicose 0,4% (Merck, Darmstadt, Alemanha, Ref. K25252846 831), extrato de malte 1% (Carl Roth, Karlsruhe, Alemanha, Ref. X976.2), extrato de levedura 0,4% (Merck, Darmstadt, Alemanha, Ref. 1.11926.1000), pH 6,3. 3) PDB (Caldo de Dextrose de Batata): 2,0% de D-glicose, 0,4% de purê de batatas (Pfanni, Hamburg, Alemanha). 4) Meio de farinha de milho (CM): 2,0% de fubá (Neuform, Zarrentin, Alemanha), 1,0% de D-glicose. 5) GM1 (meio de Glicose-Levedura 1): 2,0% de D-glicose, 0,5% de extrato de levedura. 6) GM2 (meio de Glicose-Levedura 2): 2,0% de D-glicose, 0,1% de extrato de levedura. 7) Meio de malte 1: 2,0% de extrato de malte, 0,5% de extrato de levedura. 8) Meio de malte 2: 2,0% de extrato de malte sem extrato de levedura. 9) Meio de suco de maçã: 10% de suco de maçã (produto comercial comum: suco claro, EAN 20009717, Fruchtstern®, marca registrada da Netto Marken-Discount AG & Co.KG, Maxhütte-Haidhof, Alemanha), 4,83% de glicose, 5,94% de frutose, 0,23% de sacarose, 0,1%(v/v) de solução de dureza (preparada a partir de 4,4 g de CaCl2*2H2O + 3,04 g de MgCl2*6H2O dissolvidos em 100 ml de água), 0,2%(v/v) de malato de sódio a 1M, 0,2%(v/v) ácido maléico a 1M; ajustado para pH 3,2 - 3,4 com ácido maléico a 1M. 10) Meio de Tripticase Soja Extrato de Levedura: 3% de Caldo de tripticase soja (Difco, Lawrence EUA, Ref. 211825), 0,3% de Extrato de levedura (Merck, Darmstadt Alemanha, Ref. 111926). [000470] The following Examples illustrate the invention, without limiting its scope.
[0471] [000471] One ml of a cryoprob containing a mycelium suspension of FU50088 in 10% glycerol was recovered from liquid nitrogen and, after thawing, used to inoculate 200 ml Erlenmeyer flasks containing 50 ml of sterile YMG medium and propagated on a shaker rotating at 240 rpm and 23 ° C for 72 h. After that, each two ml of the primary seed culture were transferred to batches of two 500 ml Erlenmeyer flasks containing 200 ml of the same medium and propagated on a rotary shaker at 140 rpm and 23 ° C for 120 h. These vials served as secondary seed cultures. b) Fermentation on a 301 scale
[0472] [000472] A 40 l Biostat LP42 fermenter (Bioengeneering, Wald, Switzerland) containing 30 l of medium was sterilized in situ (1 h at 121 ° C and 110 kPa (1.1 bar)) and inoculated with 400 ml of the culture of secondary seed. The production culture was developed under agitation (240 rpm) and aeration (0.2 vvm (volumes of air per minute per batch volume)) at 30 ° C.
[0473] [000473] In addition, at each incubation time point listed above, HPLC analysis of crude extracts, prepared from 20 ml samples obtained under sterile conditions and extracted with equal amounts of ethyl acetate, served as a means detection and estimation of glycolipids. For this purpose, the ethyl acetate extracts are dried over anhydrous sodium sulfate, evaporated to dryness, dissolved again in 2-propanol and analyzed using the HPLC systems described below in the "fermentation control" HPLC-MS methods.
[0474] [000474] For the optimization of culture media, Dacryopinax spathularia was propagated in a series of batches of 500 ml shake flasks each containing 200 ml of culture media as described above (Example 1 B) under "a ) Shake flask cultures "(including" Seed cultures "). During fermentation, the samples were obtained, and the pH, the dry weight of the mycelium, the amount of extractable material and the biological activities of the crude ethyl acetate extracts from the culture broth against Bacillus subtilis and Zygosaccharomyces bailii were determined in the agar diffusion. The results (Table A) show that, although glycolipids are produced in a variety of different culture media, the GM2 medium showed the highest specific biological activity in relation to dry mycelium weight and fermentation time. As it could also be observed during the fermentation by microscopic control that the glycolipids adhered to the mycelium hyphae during the production phase, it was considered favorable to use the GM2 medium for large-scale fermentation, to drive most of the products to be located in the culture broth, especially in view of the precipitation experiments that finally found the most effective and ecologically beneficial way to obtain the desired compounds. Furthermore, maximum production was achieved earlier than in most other fermentation media. c) Fermentation on a 200 I scale
[0475] [000475] Fermentation was carried out in a 300 l fermentor (Bioengineering Type P, equipped with four Ekato Intermig® impellers) containing 200 l of GM2 medium, sterilized under steam for 45 min at 121 ° C, inoculated with the seed culture 20 l described above. To prevent foaming, 0.03 ml / l of Clarol FBA 3003K (Cognis, Monheim, Germany) as a defoaming agent was added; no additional supply of antifoam was required during fermentation. Fermentation was carried out at around 33 ° C, under agitation (75 rpm) and aeration (0.2 vvm). Fermentation was stopped after 300 h, when the free glucose had almost been consumed and the partial pressure of oxygen had dropped to 20%. C) Preparation of extracts a) Preparation of mycelium extract
[0476] [000476] Cultures from 10 shake flasks were collected. The culture fluid was separated by filtration of the mycelia. The wet mycelia were extracted twice with equal volumes of acetone for every 30 min in an ultrasonic bath. This acetone was evaporated in vacuo at 40 ° C and the remaining aqueous phase was diluted to 700 ml with water. This phase was extracted three times with equal volumes of ethyl acetate (EtOAc). The combined organic phases were dried over anhydrous Na2SO4 and evaporated in vacuo to yield 329 mg of a crude extract. b) Preparation of the culture fluid extract
[0477] [000477] 1000 g of Amberlite® XAD16 (Sigma-Aldrich, St. Louis, MO 63103, CAS 90003-69-4, Batch No. 099K0079) was added to 30 l of culture filtrate (from a 30 1, GM2 medium) and incubated overnight with shaking (60 rpm). The resin was collected by filtration and the dried resin was incubated with two liters of methanol and incubated for 30 min in an ultrasonic bath. After that, the methanol eluate was removed by filtration. This methanol elution process was repeated twice. The methanol eluates (about 6 l) were evaporated combined in vacuo and the resulting oily residue dissolved again in 500 ml of distilled water. The pH was adjusted with 2 M HCl to pH 2.9, and the resulting suspension was extracted three times each with equal amounts of ethyl acetate. The combined organic phases were dried over sodium sulfate, evaporated in vacuo (40 ° C) to produce 25 g of a light brown, amorphous crude extract. c) Preparation of a sedimentation product (precipitation) (Downstream processing)
[0478] [000478] The culture broth resulting from a 200 l fermentation was basified from an initial pH value of 4.5 to pH 8 with 1 N sodium hydroxide solution, to allow the glycolipids that partially adhere to the cells, under acidic conditions, become largely released from mycelia. After 1 h, the mycelia were separated using a Westfalia KA1-06-525 separator and, in addition, the culture broth was filtered through a Pall microfiltration system (Dreieich Germany) 0.1 polysulfone membrane filter cassettes µm, fiber diameter 1.4 mm, total area 24 m2) to completely retain mycelia. The product was pelleted (precipitated) by acidifying the filtrate with 2 N hydrochloric acid to pH 3 and incubating for 16 h under cooling to 11 ° C. The fluid was removed by decanting and subsequent centrifugation (4500 rpm, 15 min, Typ Jouan SA LR 5.22 (Jouan, Paris France), resulting in a light gray gel. This crude product was immediately washed with water (pH 7), centrifuged again (at 4500 rpm for 15 min) and lyophilized This process produced 380 g of dry glycolipid which was further characterized by HPLC-MS (see, for example, the HPLC chromatogram in Fig. 2). D) Isolation of compounds a) Flash chromatography
[0479] [000479] 10 g of the crude product were dissolved in 5 ml of methanol and connected to 10 g of Chromabond XTR (Diatomaceous earth for the extraction of liquid-liquid Macherey-Nagel, Article No. 730 595,500, Düren, Germany) and the solvent was evaporated in vacuo. This dried material was applied to an MPLC Biotage Isolera system (Uppsala, Sweden), using a Chromabond® flash (120 Nucleodur 100-20 C18ec; 130 x 40 mm) (trademark of Macherey-Nagel) as a stationary phase.
[0480] [000480] The column was equilibrated with ACN / water (1: 5) and then eluted using the following conditions in a flow of 20 ml / min: 3:30 min, ACN / water (1: 5) isocratic; 9 min, ACN / water gradient (1: 1); 19:00 min, ACN / water (1: 1) isocratic; 29:00 min ACN / water gradient (2: 1); 49:00 min ACN / water (2: 1) isocratic; 59:00 min gradient from pure ACN.
[0481] [000481] Small aliquots of the fractions were obtained, evaporated and dissolved in 2-propanol to concentrations of 5 mg / ml and analyzed by HPLC-MS using the "Adapted Method" as described in Example 3. The fractions were combined according to HPLC-MS results and concentrated in vacuo.
[0482] [000482] The fractions containing the compounds of formula I elute according to the following table. Table 2: Chromatographic elution profile of compounds of formula I
[0483] [000483] All these separation steps were carried out with CA: Waters SunFire C18 preparative HPLC column (7 μm length 250 mm * diameter 19 mm) CB: Kromasil C18 (7 μm length 250 mm * diameter 40 mm) + pre-column (Kromasil C18, 7 μm, length 50 mm * diameter 20 mm) CC: Kromasil C8 (7 μm, length 250 mm * diameter 40 mm) CD: Inertsil ODS-3 C18 (5 μm, length 250 mm * diameter 40 mm) as a stationary phase. The eluent was established with ACN and water using a flow rate of 10 ml / min. The maximum capacity of this column is about 600 mg. Therefore, it was necessary to perform several identical purifications in series to purify larger quantities.
[0484] [000484] The separations were monitored by a diode array detector at 200 and 210 nm. Five ml fractions were obtained using an automatic fraction collector and finally combined according to UV absorption (200 and 210 nm), concentrated in vacuo and subjected to HPLC-MS, to evaluate its purity.
[0485] [000485] For those skilled in the art, it is obvious to adapt the elution methodology to the retention needs of each compound, for example, the less polar compounds will elute later, consequently it is possible to start with a higher content of ACN in the eluent. Sometimes it is better to use another gradient slope or it is necessary to use an isocratic system.
[0486] [000486] Illustratively, the purification of compound [1] is described in this document, in detail: The elution profile was run from: 10 min, 50% isocratic ACN; gradient of 30 min, ACN 50% ⟶ 80%; 45 min, ACN 80% isocratic; 50 min gradient, ACN 80% ⟶ 85%; 70 min, ACN 85% isocratic; 90 min gradient, ACN 85% ⟶ 100% with a flow rate of 20 ml / min using the CB column (Kromasil C18). Within a retention time of 35--36 min, compound [1] eluted from the system described above. Purity was determined to be 95% by 1H-NMR. E) Definition of extracts
[0487] [000487] The extracts containing the compounds of formula I are defined with their preparation procedures, for example, the use of the processes explained above. Table 4:
[0488] [000488] Illustratively, the encoding of [X1] is described in this document in detail: The use of strain FU50088 of the species Dacryopinax spathularia in a fermentation procedure, as described above in example 1 "Cultivation", with a process as described in this example under section B "fermentation", using a 30 l fermentor as described in this section under subsection b) "301 fermentations", followed by a preparation procedure, as described in the same example 1, with a process as described under section C "Preparation of extracts", using a precipitation process, as described in this section under subsection c) "Preparation of a sedimentation product", is coded as "an extract of FU50088 according to example 1 using a process of production B b) and an extraction process C c) "or in short" extract FU50088 example 1, B b), C c) ". Example 2: Structural characterization Compound No. [1]
[0489] [000489] The molecular structure was elucidated by complete interpretation of the high resolution mass spectrometric data and the 1D and 2D NMR spectra. The structural characterization follows the general methodology that is known to the person skilled in the art and described in more detail in the scientific literature (examples: Nishida et al., J. Antibiot. 1991, 44, 541; Nishida et al, Chem. Pharm. Bull, 1991, 39, 3044).
[0490] [000490] The numbering of the atoms is shown in Fig. 1. Chemical Formula: C49H88O21 bake Exact: 1012.5818 From Molecular Weight: 1013.2104 From HR-ESIMS: found m / z 1013.5874; calculated m / z 1013.5891 for [M + H] +
[0491] [000491] NMR spectra were obtained on CD3OD at 293 K on a Bruker DRX spectrometer operating at a proton frequency of 500 MHz. The residual peak of solvent was used as an internal reference (δh = 3.30; δc = 49.0 ). The assigned NMR data is summarized in Tables 5-14.
[0492] [000492] LC-MS / UVELSD analyzes were performed using an Agilent HP1100 liquid chromatograph (Agilent, Waldbronn, Germany) coupled with an LCT mass spectrometer (Waters Corporation, Milford, MA, USA) in positive electrospray ionization mode and negative (ESI) and a Sedex 75 Evaporative Light Diffusion Detector (Sedere, Alfortville Cedex, France). A Waters symmetry column (Waters Symmetry® (Trademark by Waters) C18, 3.5 µm, 2.1 mm x 150 mm, Waters GmbH, Eschborn, Germany) was used as the stationary phase, with a flow rate of 0, 4 ml / min at 40 ° C. Mobile phase A: 0.1% formic acid in water, mobile phase B: 0.1% formic acid in acetonitrile; gradient: 0-1 min. 98% A, 1-21 min. up to 100% B, from 21-27 min 100% B. The UV / Vis spectra were recorded between 200-500 nm, the LC-MS spectra (Liquid Chromatography-Mass Spectrometry coupling) were recorded in the range molecular weights between 160 and 1,600 Da. b) "Adapted method"
[0493] [000493] LC-MS / UVELSD analyzes were performed using an Agilent HP1100 liquid chromatograph (Agilent, Waldbronn, Germany) coupled with an LCT mass spectrometer (Waters Corporation, Milford, MA, USA) in positive electrospray ionization mode and negative (ESI) and a Sedex 75 Evaporative Light Diffusion Detector (Sedere, Alfortville Cedex, France). A Waters symmetry column (Waters Symmetry® (Trademark by Waters) C18, 3.5 µm, 2.1 mm x 150 mm, Waters GmbH, Eschborn, Germany) was used as the stationary phase, with a flow rate of 0, 4 ml / min at 40 ° C. Mobile phase A: 0.1% formic acid in water, mobile phase B: 0.1% formic acid in acetonitrile; gradient: 0 min: 55% A, 0-14 min. up to 100% B, from 14-16 min 100% B. The UV / Vis spectra were recorded between 200-500 nm, the LC-MS spectra (Liquid Chromatography-Mass Spectrometry coupling) were recorded in the range molecular weights between 160 and 1,600 Da. c) "Fermentation control"
[0494] [000494] HPLC system: Agilent 1100 analytical HPLC system including pumps and autosampler, DAD detectors (200-500 nm) and ELSD: column oven at 40 ° C; column: Waters Symmetry® C18 3.5 µm (2.1 x 150 mm); solvents: deionized water (A) and acetonitrile (B) with 0.1% formic acid each. The flow was adjusted to 0.4 ml / min using a temperature of 40 ° C. The applied gradient was optimized for the separation and resolution of the glycolipid pattern: 0 to 14 min: from 45% to 100% (B); 14 to 16 min: 100% (B); 16 to 16.1 min: from 100% to 45% (B); 16.1 to 20 min: 45% (B). For HPLC analysis, the samples were dissolved in 2-propanol. B HPLC-MS of pure compounds Table 15: Retention times and MS signals for compounds of formula I, HPLC prepared according to the methods specified in Section A of this Example.
[0495] [000495] All signals were unequivocally attributed to being glycolipids by HPLC-MS / UV. b) Extracts of the strain Dacryopinax spathularia MUCL53181 Extract [X1]: Mixture of glycolipids prepared according to Example 1 from 30 l of fermentation Extract [X2]: Mixture of glycolipids prepared according to Example 1 from 200 l of fermentation Table 17: Analysis by HPLC-MS (using the "Adapted Method") of the extracts obtained from the strain Dacryopinax spathularia MUCL 53181 (the HPLC-ELSD chromatogram for [X2] is shown in Figure
[0496] [000496] The various bacteria, yeasts, and non-pathogenic fungi were obtained from collections of public cultures and maintained as recommended in the catalogs and protocols of the respective institutions. The strain Saccharomyces cerevisiae HT10 and Mucor plumbeus were originally obtained from the culture collection of InterMed Discovery GmbH, but deposited with MUCL as reference strains for the antimicrobial susceptibility test. These strains were kept under liquid nitrogen and, before the exam, on YMG agar.
[0497] [000497] Before the examination, yeast and bacterial strains were grown overnight on SDB (medium 1), except for Bacillus subtilis, which was grown on YMG agar (medium 2), for 1 week, to prepare the suspensions of spores. Also, the filamentous fungi were pre-incubated on YMG agar for 2-3 weeks to create inoculum for the spore suspensions. The spores were then rinsed from the surface of the flasks using 0.9% saline, checked for viability by microscopic control and preparation on agar plates, and diluted to the desired spore concentration. For all experiments, freshly prepared spore suspensions were used. The initial concentrations for the bioassays were adjusted to 1 x 105 CFU (i.e., cells or spores, respectively) per ml. The standards (preservative agents benzoic acid and sorbic acid; antibiotics: penicillin G, streptomycin sulfate, amphotericin B) served as positive controls.
[0498] [000498] To adjust the initial titer before the bioassays, the CFU per ml was determined under the microscope using a Brand Neubauer improved counting chamber; BRAND GmbH & Co KG, Wertheim, Germany). This microscopic control also served as a means for assessing the viability of cells.
[0499] [000499] The actual tests were performed on Bio-one suspension culture plates type Greiner (96 Wells, flat bottom, sterile, No 655185).
[0500] [000500] The compounds or extracts to be tested from the stock material, including the standards, were dissolved in appropriate volumes of DMSO prior to testing and diluted in the microtiter plates, using a final concentration of 1.5%. Apart from the regular incubation time for determining the MIC (18-24 h), the stability of the inhibitory effects was also studied in prolonged incubation times. For this purpose, each vial on the microtiter plate was filled with 200 μl of the cell suspensions and the test plates were incubated in an incubator (Heraeus HERA cell) at 28 ° C, an absolute humidity of 95%, to prevent evaporation of the solvent. Under such conditions, no noticeable evaporation of the microtiter plates was observed for up to several weeks. MICs were generally determined in a traditional way, checking the MTP optically and determining the dilution of each individual compound where no visible growth has occurred. However, the DO630 was also determined using a plate reader, in these cases where it seemed difficult to observe the MIC with the naked eye. In some situations, the DO630 of the plates could be monitored and determined using the plate reader for up to four weeks. However, in general, long-term experiments have been run for at least 168 hours. For the determination of DO630, microtiter plates were scanned using an SPECTROstar Omega plate reader (BMG LABTECH, Offenburg, Germany), except for filamentous fungi, where a PHERAstar plus plate reader (BMG LABTECH, Offenburg, Germany) used in "Wellscan" mode (action sphere averaging 4 mm), as this instrument provided more reliable data if the mycelium colonies originated from the initial spore suspensions. The MIC on the optical viewfinder was determined using the following formula:
[0501] [000501] The MIC values described refer to the concentration that causes at least 80% inhibition compared to the positive control. Results: Table 18: MIC values of standards in different media: sorbic acid [SA] and benzoic acid [BA]
[0502] [000502] Note: The apple juice medium per se already shows limited growth, which is mainly based on the low concentration of nitrogen compounds available and necessary for growth.
[0503] [000503] It is evident and notable that, regardless of the extraction method (for example, X1 - X3), comparable results are achieved. Bacteria Bacillus subtilis (ATCC6633) Clostridium perfringens (ATCC13124) Corynebacterium variabile (DSM20132) Corynebacterium variabile (ATCC15753) Escherichia coli (ATCC9637) Lactobacillus plantarum (DSM12028) Pseudomonas putida (ATCC17484) Staphylococcus aureus (ATCC 6538P) Filamentous fungi ('molds') Aspergillus fumigatus (ATCC1028) Aspergillus niger (ATCC16404) Byssochlamys fulva (DSM62097) Mucorplumbeus (MUCL49355) Yeasts Dekkera bruxellensis (DSM70726) Dekkera naardenensis (DSM70743) Saccharomyces cerevisiae (HT10) Zygosaccharomyces bailii (DSM70492) Zygosaccharomyces bailii (ATCC60484) Zygosaccharomyces bisporus (DSM70415) Zygosaccharomyces florentinus (DSM70506) Zygosaccharomyces rouxii (NCYC381) b) Pathogenic microorganisms
[0504] [000504] Several samples of extracts and pure compounds were tested against Staphylococcus aureus (ATCC 6538P), Clostridium perfringens (ATCC 13124) or Aspergillus fumigatus (ATCC 1028). The test was performed by Ricerca Biosciences, LLC (Taiwan): S. aureus (cat # 604000) with Mueller-Hinton Broth medium using 1% DMSO as a vehicle for an incubation time of 20 hours at 36 ° C (di Modugno , Antimicrob Agents Chemother (1994) 38: 2362-8); C. perfringens (cat no 620700) with Reinforced Clostridium Medium using 1% DMSO as a vehicle for an incubation time of 2 days at 36 ° C (di Modugno, ibid); A. fumigatus (cat no 640010) with Potato Dextrose Broth medium using 1% DMSO as a vehicle for an incubation time of 3 days at 28 ° C (Turner, Antimicrob Agents Chemother (1989) 33 (2): 215 -22). MICs were detected by measuring turbidity in all cases.
[0505] [000505] The average mol weight was adjusted to 1000 g / mol, the data shown in table 22 below are given in mg / ml (ppm). Table 22: MICs [pm] of extracts and pure compounds against pathogenic microorganisms.
[0506] [000506] The extract [X2] was dissolved in water to final concentrations of 10 ppm and 100 ppm. These two test samples were presented together with a sample of pure water (as a negative control) to two test persons. The three samples were blinded before the taste evaluation.
[0507] [000507] None of the test persons was able to distinguish between pure water and the sample containing 10 ppm of the test compound. The sample containing 100 ppm of the test compound was described with a diffuse taste comparable to the water that was stored for a longer time in an open PET bottle. No bitter, strong or otherwise unpleasant taste was observed.
[0508] [000508] In a second test, a test person tried the pure dry powder of the aforementioned test compound. Even after this application, no additional adverse or unpleasant taste was mentioned. Example 6: Optical Rotation Values
[0509] [000509] Optical rotation values were determined in methanolic solutions using a Unipol L 1000, Schmidt-Haensch polarimeter, equipped with a silica glass microcube (100 mm long; 1 ml sample volume). Table 23: Specific values of rotation in methanol
[0510] [000510] A non-aerated drink based on 2% fruit juice, pH 3.4 and about 12 Brix, is formed by combining the following materials.
[0511] [000511] A pH of 3.4 is reached through the combinations of malic acid and sodium malate. The total combined amount of sodium malate and malic acid is almost constant, but the ratio of malic acid and malate varied slightly depending on the content of compound extract [X2].
[0512] [000512] The above mentioned mixture is inoculated with the test organisms and incubated for weeks at room temperature. Example 7: Semi-finished products A) The following mixtures can be used as concentrates for preservative activities in different foods or drinks.
[0513] [000513] The compound extract [X2] is used as a concentrated solution (concentrate) in DMSO, which will be diluted to a final DMSO content of the concentrate in the semi-finished products. The compound extract solution [X2] is pre-mixed with the appropriate alcohol, in variation C, together with orange oil, in variation B, together with beeswax, while the thickeners are mixed with an appropriate volume of Water. The two mixtures are vigorously stirred, combined while continuing to stir and filled with water to the final volume (100%). B) The following mixture is to be used as a concentrate for an apple juice drink (9 liters).
[0514] [000514] Semi-finished products are tested against microorganisms, for example, fungus, yeasts and / or bacteria. Example 8: Production and isolation of a mixture of compounds of formula I and a mixture of sodium salts of compounds of formula I A) Fermentation using the FU50088 strain (Dacryopinax spathularia MUCL 53181 strain) a) Seed culture (Shake Flask Cultures)
[0515] [000515] One ml of a cryoprote containing a mycelium suspension of the MUCL 53181 strain in 10% glycerol was recovered from liquid nitrogen and, after thawing, used to inoculate 200 ml Erlenmeyer flasks containing 50 ml of sterile YMG medium and propagated over a rotary shaker at 240 rpm and 23 ° C for 72 h. After that, each two ml of the primary seed culture were transferred to batches of two 500 ml Erlenmeyer flasks containing 200 ml of the same medium and propagated on a rotary shaker at 140 rpm and 28 ° C for 90 h. These vials served as secondary seed cultures. b) Fermentation on a 30 I scale
[0516] [000516] A 40 l Biostat LP42 fermenter (Bioengeneering, Wald, Switzerland) containing 30 l of GM2 medium was sterilized in situ (1 h at 121 ° C and 110 kPa (1.1 bar)) and inoculated with 1500 ml of the culture secondary seed. The production culture was developed under agitation (240 rpm) and aeration (0.2 vvm (volumes of air per minute per batch volume)) at 30 ° C for 200 h. B) Downstream processing and insulation
[0517] [000517] The culture broth resulting from the 30 l fermentation of Example 8 A) b) was alkalized from an initial pH value of 4.5 to a pH value of 8 with 5 N sodium hydroxide solution , to allow glycolipids that partially adhere to cells, under acidic conditions, to become largely released from mycelia. After 1 h, the mycelia were separated from the culture broth by centrifugation, and then the culture broth was further filtered through a Pall T1000 depth filter (Dreieich, Germany) to remove cell clumps and filamentary material. The product was pelleted (precipitated) by acidification of the filtrate with 6 N hydrochloric acid to pH 2.2 and subsequent storage for 20 h at 4 ° C. The fluid was removed by decanting, subsequent centrifugation (4500 rpm, 15 min, type Jouan SA LR 5.22 (Jouan, Paris, France) and then discarded, resulting in a light gray gel. This crude product was washed immediately with 1 l of water slightly basic demineralized (adjusted to a pH value of 8 with sodium hydroxide) and centrifuged (at 4500 rpm for 15 min), the supernatant was removed and the remaining pellet was suspended in 0.5 l of demineralized water. the residue was lyophilized to produce a slightly beige-gray powder (residual water content: 1.36% (according to Karl Fischer method), the total protein content was below 1% (<1%) (method de Kjeldahl according to ISO 5549: 1978) .This process produced a total of 87 g of glycolipids, which were subsequently characterized by HPLC-MS (see [X7] in Table 24). B-2) Isolation of a mixture of sodium salts of the compounds of formula I
[0518] [000518] The culture broth resulting from the 30 l fermentation of Example 8 A) b) was alkalinized from an initial pH value of 4.5 to a pH value of 8 with 5 N sodium hydroxide solution , to allow glycolipids that partially adhere to cells, under acidic conditions, to become largely released from mycelia. After 1 h, the mycelia were separated from the culture broth in a separator, and then the culture broth was pumped through a combined filter unit: first through a depth filter with a pore size of 0.65 μm and then through a membrane filter with a pore size of 0.45 µm, to remove not only filamentary material and cell clusters, but also cells. The product was pelleted (precipitated) by acidifying the filtrate with 6 N hydrochloric acid to pH 2 and then stored for 16 h at 4 ° C. The fluid was removed by decanting, subsequent centrifugation (4500 rpm, 15 min, Type Jouan SA LR 5.22 (Jouan, Paris, France) and then discarded, resulting in a light gray gel. This crude product was immediately washed with 1 l of water demineralized and centrifuged (at 4500 rpm for 15 min.) The supernatant was removed, the remainder suspended in 0.5 l of demineralized water and the pH adjusted to a pH value of about 6 with 5 N sodium hydroxide solution Finally, the resulting solution was lyophilized to produce a total of 93 g of sodium glycolipid salts in dry form as a slightly beige powder (residual water content: 1.2% (according to Karl Fischer method), the total protein content was below 1% (<1%) (Kjeldahl method according to ISO 5549: 1978). C) Analysis by HPLC-MS - "Improved method"
[0519] [000519] HPLC-MS analyzes were performed using a Dionex Ultimate® 3000 RSLC liquid chromatograph (Thermo Fisher GmbH, Idstein; Germany) coupled with an amaZon SL ion capture mass spectrometer (Bruker Daltonik GmbH, Bremen, Germany) in the negative electrospray ionization mode (ESI) and an ELSD Sedex 85 (Sedere, Alfortville Cedex, France). A Nucleoshell RP18 column (2.7 µm, 2 mm x 150 mm, Macherey-Nagel GmbH & Co. KG, Düren, Germany) was used as the stationary phase, with a flow rate of 0.4 ml / min at 40 ° C . Mobile phase A: 0.1% formic acid in water, mobile phase B: 0.1% formic acid in acetonitrile; gradient: 0 min: 70% A, 0-40 min. up to 40% A, 40-42 min up to 0% A, 42-48 min. 0% A, 48-49 min to 70% A, 49-55 min 70% A. The LC-MS spectra (Liquid Chromatography-Mass Spectrometry coupling) were recorded in the molecular weight range between 700 and 1,200 Da. Table 24: HPLC-MS analysis (using the "improved method") of the extract [X7] obtained from the strain Dacryopinax spathularia MUCL 53181 according to Example 8 B-1)
[0520] [000520] A 1 liter Erlenmeyer flask containing 400 ml of sterile medium (2.0% D-glucose, 0.5% malt extract) was inoculated with 2 ml of the secondary seed culture of Example 8 A a) . The production culture was developed on a rotary shaker (at 200 rpm), at 32 ° C, for 360 h.
[0521] [000521] The preparation after 360 h of fermentation produced a total of 5.6 g / l of glycolipids of formula I, which were subsequently characterized by HPLC-MS (see [X8] in Table 24A). Table 24A: HPLC-MS analysis (using the "improved method" of Example 8 C)) of the extract [X8] obtained from the strain Dacryopinax spathularia MUCL 53181 according to Example 8 D) (the HPLC-ESI chromatogram for [X8] is shown in Figure 4)
[0522] [000522] A 1 liter Erlenmeyer flask containing 400 ml of sterile medium (4.0% D-glucose, 0.2% yeast extract) was inoculated with 6 ml of the secondary seed culture of Example 8 A a) . The production culture was developed on a rotary shaker (at 200 rpm), at 32 ° C, for 240 h.
[0523] [000523] The preparation after 240 h of fermentation produced a total of 6.2 g / l of glycolipids of formula I. Example 9: Biological activities
[0524] [000524] In analogy to the methods described in Example 4 above, additional MIC data of biological activity were determined for several pure compounds (purity> 94% by weight) and for the mixture of compounds of formula I (extract [X8] as described in detail in Example 8 D).
[0525] [000525] Tables 25 - 27 below represent the corresponding MIC values of several pure compounds after 48 h against several microorganisms.
[0526] [000526] In summary, Tables 25 - 27 demonstrate that individual compounds of formula I without any acyl substituents in the carbohydrate portion of trisaccharide R (as is the case for compound [16])) typically exhibit significantly more antimicrobial activity weaker, particularly against yeasts and fungi, than the corresponding compounds being mono- or diacylated in the saccharide portion.
[0527] [000527] Said Tables also demonstrate that the individual compounds of formula I with an acyl substituent with more than 2 carbon atoms, such as an isovaleryl substituent, in the carbohydrate portion of trisaccharide R (as is the case for compounds [12], [13] and [1]) typically exhibit stronger antimicrobial activity, particularly against yeasts and fungi, and / or a broader spectrum of activity than the corresponding compounds with an acetyl substituent on the carbohydrate portion of trisaccharide R (such as compound [14]). Table 25: MIC values [pm] after 48 h of pure compounds against Gram-positive bacteria
[0528] [000528] Table 28 below represents the MIC values [pm] of the mixture [X8] and the pure compound [12] after 48 h against various microorganisms.
[0529] [000529] At a concentration of 25 ppm of [X8], the observed inhibition of Bacillus cereus was 90%. At a concentration of 12.5 ppm of [X8], the observed inhibition of Candida albicans was 90-100%. At a concentration of 50 ppm of [X8], the observed inhibition of Clostridium sporogenes was 80-90%. At a concentration of 100 ppm of [X8], the observed inhibition of Staphylococcus aureus was 90%.
[0530] [000530] Table 29 below shows the results of several tests of bacterial counts carried out with the mixture [X8] for several microorganisms, compared to an untreated control. The tests were carried out at 37 ° C at a pH of 7.4 in complete medium. The respective bacterial count (Ba.C) is indicated in colony forming units / ml (CFU / ml). Table 29: Tests of bacterial counts carried out with the mixture [X8]
[0531] [000531] The beverage compositions A and C, each, were carbonated with 3.8 volumes of carbon dioxide after filling in the bottles. The beverage compositions B and D each were carbonated with 3.0 volumes of carbon dioxide after filling into the bottles.
[0532] [000532] The beverage compositions A and C, each, were carbonated with 4 volumes of carbon dioxide after filling in the bottles. Drink composition B was carbonated with 2 volumes of carbon dioxide after filling into the bottles. Example 13: Yogurt drink compositions
[0533] [000533] Skim milk and whole milk were mixed in proportions to give a milk with 1.1% fat, then 5% by weight of sucrose was added to it, and heated to 82 ° C for 30 minutes. After cooling to 42 ° C, 0.7% of a commercially available starter culture of Bifidobacterium bifidum and 0.5% of a starter culture of Streptococcus thermophilus were added, and the mixture grown at 39 ° C to pH of the mixture reaches 4.4. The resulting firm yogurt rennet was then broken by shaking, and divided into two portions (portion A and portion B).
[0534] [000534] Preparation of LiqYog A: To a stirred portion A was added 0.4% by weight (based on the mass of firm yogurt) of citric pectin with high methoxyl content as a 5% by weight solution in water and the cooled mixture with stirring to 5 ° C. This product was then passed through a sterilized homogenizer at 4000 kPa (40 bar), to give a liquid yogurt having a dynamic viscosity of 380 mPas at 10 ° C. 45 ppm of the extract [X7] of Example 8 B-1) were first added to it, and then 9.0% by weight of a pasteurized peach pulp, in both cases based on the total weight of the yogurt liquid. The resulting mixture was homogenized, giving LiqYog A, which was transferred to a glass container, and stored at 7 ° C for 10 days.
[0535] [000535] Preparation of LiqYog B: To a stirred portion B was added 0.6% by weight (based on the mass of firm yogurt) of apple pectin with high methoxyl content as a 5% by weight solution in water and the mixture cooled with stirring to 5 ° C. This product was then passed through a sterilized homogenizer at 1500 kPa (15 bar), to give a liquid yogurt having a dynamic viscosity of 600 mPas at 10 ° C. 95 ppm of the extract [X8] of Example 8 D) was first added to it, and then 7.5% by weight of a pasteurized strawberry purée, in both cases based on the total weight of the yogurt liquid. The resulting mixture was homogenized, giving LiqYog B, which was stored at 8 ° C before further processing. Example 14: Mouthwash fluids
[0536] [000536] Emulsions / lotions A, C and D were each applied separately to a non-woven fabric to produce wet towels (pre-moistened towels). Solution B was applied to sheets of woven fabrics.
[0537] [000537] Compositions A - D were each applied separately to a nonwoven fabric to produce wet towels.
[0538] [000538] Compositions A and C are sun sprays, composition B is a soft sunscreen cream. Example 24: Composition for hand sanitization
[0539] [000539] A clear liquid composition having a pH value of 5.6 was prepared, consisting of 2-propanol (45% by weight), 1-propanol (30% by weight), lactic acid (0.3% by weight) ), 1-tetradecanol, medium chain triglycerides, glycerol, sodium lactate, extract [X7] from Example 8 B-1), and water. Example 25: Composition for hand sanitization
[0540] [000540] A clear liquid composition having a pH value of 5.3 was prepared, consisting of 1-propanol (40% by weight), 2-propanol (28% by weight), citric acid (0.2% by weight) ), lactic acid (0.15% by weight), 1-dodecanol, medium chain triglycerides, glycerol, 1,2-propylene glycol, extract [X8] from Example 8 D) (45 ppm), and water. Example 26: Composition for hand sanitization
[0541] [000541] A composition was prepared, consisting of ethanol (55% by weight), 1-propanol (10% by weight), 1,2-propylene glycol (6% by weight), 1,3-butylene glycol, lactic acid , extract [X8] from Example 8 D) (15 ppm), and water.
[0542] [000542] Compositions A and B are concentrated, which are diluted with water in a ratio (concentrate: water) in the range of 1: 80 to 1: 20 to give a ready-to-use solution.
[0543] [000543] Compositions C and D are ready-to-use solutions.

权利要求:
Claims (9)
[0001]
Use of a compound having formula I or a mixture of two or more compounds having formula I,
[0002]
Use according to claim 1, characterized by the fact that the material to which the agent is added is a cosmetic, a food or a drink.
[0003]
Use according to claim 1, characterized in that the compound of formula I is or the mixture of compounds of formula I comprises at least one compound selected from the group of compounds with the following formulas:
[0004]
Use according to claim 1, characterized by the fact that at least one additional preservative is added.
[0005]
Use according to claim 1, characterized by the fact that the compound or compounds of formula I, or physiologically acceptable salt thereof, are added in the form of an extract from a natural source or obtained from such an extract.
[0006]
Use according to claim 5, characterized by the fact that the source of the extract is the strain Dacryopinax spathularia FU50088 or strain Femsjonia luteo-alba (= Ditiola pezizaeformis) MUCL 53500.
[0007]
Method for increasing the microbial stability of a material, characterized in that it comprises adding one or more compounds having the formula I or physiologically acceptable salt thereof, as defined in any one of claims 1, 3, 4 to 6, to a material selected from the group consisting of a cosmetic, a food, a drink other than water, a pharmaceutical product, a medical device and an active packaging material.
[0008]
Material, characterized by the fact that it comprises, as or within a coating and / or as a mixture, an additive in the form of a compound having formula I or a mixture of compounds having formula I, a physiologically acceptable salt thereof, as defined in any one of claims 1, 3, to 6, wherein such material is a cosmetic, a food, a drink other than water, a pharmaceutical product, a medical device or an active packaging material.
[0009]
Material according to claim 8, characterized by the fact that the drink is selected from the group consisting of sparkling water, a juice, lemon and lime soda, ginger soda, root beer sodas, which are gaseous in the style of soft drinks, syrup, diet drinks, carbonated soft drinks, fruit juices, other drinks containing fruits that provide the flavor of fruit juices and contain more than 0% fruit juice, but less than 100% fruit juice fruits, fruit-flavored drinks, vegetable juices, drinks containing vegetables, which provide the flavor of any of the aforementioned vegetable juices and contain more than 0% vegetable juice, but less than 100% vegetable juice vegetable, isotonic drinks, non-isotonic drinks, soft drinks containing a fruit juice, coffee, tea, tea drinks prepared from concentrate, extracts, or tea powders, drinkable dairy products, hot chocolate, powder / mixtures for c hocolate, drinkable soy products, milk substitutes, alcoholic drinks, fruit milkshakes, orchata, sports drinks, energy drinks, health drinks, wellness drinks, smoothies, protein drinks, soy yogurts drinkable, low acid drinks, acidified drinks, nectars, tonics, frozen fizzy drinks, frozen soft drinks, liquid meal replacements, infant formulations, and combinations or mixtures thereof; or the material is a cosmetic selected from a cream, emulsion, lotion, gel or oil for the skin; a facial mask; a colored base, a powder for makeup, a powder after bath, a hygienic powder; a soap, a deodorant soap, a perfume, a toilet water, a cologne, a bath or shower preparation; a depilatory; a deodorant, an antiperspirant, a hair care product; a product to define; a cleaning product; a conditioning product; a hairdresser product; a shaving product; a product to make up and remove makeup from the face and eyes, a product intended for application on the lips, a product for the care of teeth and / or mouth; a product for nail care and painting, a product for intimate external hygiene, a product for sunbathing, a product for sunless tanning, a product for whitening the skin, an anti-wrinkle product, an absorbent tampon, a sanitary napkin, a diaper and a handkerchief.

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法律状态:
2018-04-03| B06F| Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]|

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2019-07-09| B07A| Application suspended after technical examination (opinion) [chapter 7.1 patent gazette]|

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2020-02-18| B06A| Patent application procedure suspended [chapter 6.1 patent gazette]|

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2020-06-30| B09A| Decision: intention to grant [chapter 9.1 patent gazette]|

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2020-08-25| B16A| Patent or certificate of addition of invention granted [chapter 16.1 patent gazette]|Free format text: PRAZO DE VALIDADE: 20 (VINTE) ANOS CONTADOS A PARTIR DE 06/06/2012, OBSERVADAS AS CONDICOES LEGAIS. |

优先权:

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申请号 | 申请日 | 专利标题

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EP11004776A|EP2532232A1|2011-06-10|2011-06-10|Long chain glycolipids useful to avoid perishing or microbial contamination of materials|

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EP11004776.8|2011-06-10|

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PCT/EP2012/002399|WO2012167920A1|2011-06-10|2012-06-06|Long chain glycolipids useful to avoid perishing or microbial contamination of materials|

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