uses of linagliptin as vasoprotective and cardioprotective antidiabetic agents

专利摘要:
USES OF LINAGLIPTIN AS ANTI-DIABETIC AGENTS V ASOPROTECTORS AND CARDIOPROTECTORS. The present invention relates to DPP-4 inhibitory adjustments to treat and / or prevent oxidative stress, vascular stress and / or endothelial dysfunction, as well as to the use of such DPP-4 inhibitors in the treatment and / or prevention of patients diabetic or non-diabetic, including groups of patients at risk for cardiovascular and / or kidney disease.
公开号:BR112013011961B1
申请号:R112013011961-6
申请日:2011-11-15
公开日:2021-02-09
发明作者:Thomas Klein；Andreas DAIBER；Odd-Erik JOHANSEN；Michael Mark；Sanjaykumar PATEL；Hans - Juergen Woerle
申请人:Boehringer Ingelheim International Gmbh；
IPC主号:

专利说明:

The present invention relates to certain DPP-4 inhibitors to treat and / or inhibit oxidative stress, as well as to the use of such DPP-4 inhibitors in the treatment and / or prevention of diabetic and non-diabetic patients, including groups of patients in risk of cardiovascular and / or kidney disease.
The present invention also relates to certain DPP-4 inhibitors to treat and / or prevent endothelial dysfunction.
The present invention also relates to certain DPP-4 inhibitors for use as antioxidants and / or anti-inflammatories.
The present invention further relates to certain DPP-4 inhibitors to treat and / or prevent oxidative stress, vascular stress and / or endothelial dysfunction (for example, in diabetic or non-diabetic patients) particularly regardless of or in addition to glycemic control.
The present invention also relates to certain DPP-4 inhibitors to treat and / or prevent oxidative stress induced by or associated with hyperglycemia (for example, in addition to glycemic control), as well as the use of such DPP-4 inhibitors in therapy antidiabetic.
The present invention further relates to certain DPP-4 inhibitors to treat and / or prevent metabolic diseases, such as diabetes, especially type 2 diabetes mellitus and / or related diseases (for example, diabetic complications), particularly in patients having or being at risk of oxidative stress, vascular stress and / or endothelial dysfunction, or diseases or conditions related or associated with them.
In addition, the present invention relates to certain DPP-4 inhibitors for the treatment and / or prevention of metabolic diseases, such as diabetes, especially type 2 diabetes mellitus and / or related diseases (for example, diabetic complications), in patients having or being at risk of disease and / or kidney disease, such as, for example, myocardial infarction, stroke or arterial occlusive disease and / or diabetic nephropathy, micro- or macroalbuminuria, or acute or chronic renal deficiency.
In addition, the present invention relates to certain DPP-4 inhibitors to treat and / or prevent metabolic diseases, such as diabetes, especially type 2 diabetes mellitus and / or related diseases, in patients having or at risk for diabetic complications. micro or macrovascular, such as, for example, diabetic retinopathy, diabetic neuropathy, diabetic nephropathy, or cardiovascular or cerebrovascular diseases (such as, for example, myocardial infarction, stroke or peripheral arterial occlusive disease).
In addition, the present invention relates to certain DPP-4 inhibitors to modulate, block or reduce the deleterious metabolic memory effect of (chronic or transient episodes of) hyperglycemia, particularly in diabetic complications.
In addition, the present invention relates to certain DPP-4 inhibitors to treat, prevent or reduce the risk of micro or macrovascular diseases that can be induced, memorized or associated with exposure to oxidative stress.
In addition, the present invention relates to a certain DPP-4 inhibitor to treat or prevent metabolic diseases, such as diabetes, especially type 2 diabetes mellitus and / or related diseases (for example, diabetic complications), in patients with or at risk for cardiovascular and / or kidney disease, particularly in those patients with type 2 diabetes who are at risk for cardio- or cerebrovascular events, such as patients with type 2 diabetes with one or more risk factors selected from A), B), C) and D): A) previous or existing vascular disease (such as, for example, myocardial infarction (eg, silent or non-silent), coronary artery disease, percutaneous coronary intervention, myocardial revascularization, ischemic stroke or hemorrhagic, congestive heart failure (eg NYHA class I or II, eg left ventricular function <40%), or peripheral arterial occlusive disease), B) relative organ damage vascular, (such as, for example, nephropathy, retinopathy, neuropathy, impaired kidney function, chronic kidney disease, and / or micro or macroalbuminuria), C) advanced age (such as, for example, age> / = 60 -70 years), and D) one or more cardiovascular risk factors selected from - advanced type 2 diabetes mellitus (such as, for example,> 10 years' duration), - hypertension (such as, for example, > 130/80 mmHg, or systolic blood pressure> 140 mmHg or in at least one treatment that lowers blood pressure), - current daily cigarette smoker, - dyslipidemia (such as, for example, atherogenic dyslipidemia, postprandial lipemia, or high level of LDL cholesterol (for example, cholesterol> / = 130-135 mf / dL), low level of HDL cholesterol (for example, <35-40 mg / dL in men or <45-50 mg / dL in women) and / or high level of triglycerides (eg> 200-400 mg / dL) in the blood, or in at least one treatment for abnormal lipids), - obesity (such as such as abdominal and / or visceral obesity, or body mass index> / = 45 kg / m2). - age ./= 40 and </ = 80 years, - metabolic syndrome, hyperinsulinemia or insulin resistance, and - hyperuricemia, erectile dysfunction, polycystic ovary syndrome, sleep apnea, or family history of vascular disease or cardiomyopathy in a patient with first degree, wherein the method comprises administering a therapeutically effective amount of the DPP-4 inhibitor, optionally in combination with one or more therapeutic substances to the patient.
In addition, the present invention relates to a certain DPP-4 inhibitor for use in a method to prevent, reduce the risk of or delay the occurrence of cardio- or cerebrovascular events, such as cardiovascular death, myocardial infarction (fatal or non-fatal) (for example, silent or non-silent IM), stroke (fatal or non-fatal), or hospitalization (for example, for acute coronary syndrome, leg amputation), revascularization procedures (urgent), heart failure or for unstable angina pectoris), preferably patients with type 2 diabetes, particularly those patients with type 2 diabetes who are at risk for cardio- or cerebrovascular events, such as patients with type 2 diabetes with one or more risk factors selected from among A), B), C) and D): A) previous and existing vascular disease (such as, for example, myocardial infarction (eg, silent or non-silent), coronary artery disease, coronary artery surgery myocardial revascularization, ischemic or hemorrhagic stroke, congestive heart failure (eg NYHA class I or II, eg left ventricular function <40%), or peripheral arterial occlusive disease), B) vascular related target organ damage (such as, for example, nephropathy, retinopathy, neuropathy, impaired kidney function, chronic kidney disease, and / or micro or macroalbuminuria), C) old age (such as, for example, age:> / = 60-70 a- nos), and D) one or more cardiovascular risk factors selected from - advanced type 2 diabetes mellitus (such as, for example,> 10 years in duration), - hypertension (such as, for example,> 130/80 mmHg , or systolic blood pressure> 140 mmHg or at least a treatment to lower blood pressure), - daily cigarette smoker, current, - dyslipidemia (such as, for example, atherogenic dyslipidemia, postprandial lipemia, or high cholesterol level LDL (e.g. LDL cholesterol> / = 130-135 mg / dL), low level of HDL cholesterol (for example, <35-40 mg / dL in men or, 45-50 mg / dL in women) and / or high level of triglycerides (for example, .200-40 mg / dL) in the blood, or at least one treatment for lipid abnormality), - obesity (such as, for example, abdominal and / or visceral obesity, or body mass index> / = 45 / m2), - age> / = 40 and </ = 80 years old, - metabolic syndrome, hyperinsulinemia or insulin resistance, and - hyperuricemia, erectile dysfunction, polycystic ovary syndrome, sleep apnea, or family history of vascular disease or cardiomyopathy in a first-degree relative, wherein the method comprises administering a therapeutically effective amount of the DPP-4 inhibitor, optionally in combination with one or more therapeutic substances, to the patient.
Furthermore, the present invention also relates to a certain DPP-4 inhibitor in a method to prevent, reduce the risk of or delay the occurrence of cardio- or cerebrovascular events, such as cardiovascular death, myocardial infarction (fatal or not) fatal) (for example, silent or non-silent MI), stroke (fatal or non-fatal), or hospitalization (for example, for acute coronary syndrome, leg amputation), revascularization procedures (urgent), heart failure or unstable angina pectoris), in patients with type 2 diabetes with vascular related target organ damage, particularly nephropathy, chronic renal function, micro or macroalbuminuria, in which said method comprises administering a therapeutically effective amount of DPP-4 inhibitor , optionally in combination with one or more other therapeutic substances, to the patient.
In addition, the present invention also relates to a certain DPP-4 inhibitor for use in a method to improve cognitive function (eg, attenuate, reverse or treat cognitive decline), enhance β cell function (eg, enhance the rate of insulin secretion derived from a 3-hour meal tolerance test, improving the function of long-term β cells, improving the daytime glucose pattern (for example, improving the ambulatory glucose profile, glycemic variability, biomarkers oxidation, inflammation or endothelial function), and / or improve the durability of glucose control according to the status of β cell autoantibody (for example, glutamic acid decarboxylase (GAD), in which said method comprises administering a therapeutically effective amount of the DPP-4 inhibitor, optionally in combination with one or more other therapeutic substances, to the patient.
In addition, the present invention also relates to a certain DPP-4 inhibitor for use in a method to prevent, reduce the risk of, slow down the progression of, delay the onset of, mitigate, reverse or treat cognitive impairment or cognitive decline. , wherein the method comprises administering a therapeutically effective amount of the DPP-4 inhibitor, optionally in combination with one or more other therapeutic substances, to the patient.
In addition, the present invention also relates to a certain DPP-4 inhibitor for use in a method to prevent, reduce the risk of, slow down the progression of, delay the onset of, mitigate, reverse or treat latent autoimmune diabetes in adults (LADA), wherein said method comprises administering a therapeutically effective amount of the DPP-4 inhibitor, optionally in combination with one or more other therapeutic substances, to the patient.
In addition, the present invention relates to a certain DPP-4 inhibitor for use in a method (for mixed purposes) of preventing, reducing the risk of, slowing the progression of, delaying the onset of, mitigating, reversing or treating disease or events cardio- or cerebrovascular (such as, for example, those described here), and prevent, reduce the risk of, slow down the progression of, delay the onset of, mitigate, reverse or treat diabetic nephropathy, in a patient who needs the same (such as, for example, a patient as described here, especially a patient with type 2 diabetes), wherein the method comprises administering a therapeutically effective amount of the DPP-4 inhibitor, optionally in combination with one or more of other therapeutic substances, to the patient.
In addition, the present invention relates to one or more of the following methods to - treat, reduce, prevent and / or protect against oxidative stress, such as, for example, oxidative stress induced by or associated with non-diabetes or diabetes (hyperglycima) ; - treating, preventing, reducing the risk of, slowing the progression of, delaying the onset of, attenuating or reversing endothelial dysfunction or improving endothelial function; - treating, preventing, reducing the risk of, slowing the progression of, delaying the onset of, mitigating or reversing diseases or conditions associated with oxidative stress, such as those described here; - treat, prevent, reduce the risk of, slow down the progression of, delay the onset of, mitigate or reverse (ischemia (renal, cardiac, cerebral or hepatic / reperfusion injuries and / or reduce the size of myocardial infarction (for example , after myocardial ischemia / reperfusion); - treating, preventing, reducing the risk of, slowing the progression of, delaying the onset of, attenuating or reversing (adverse) vascular remodeling such as cardiac remodeling (particularly after myocardial infarction), which can be characterized by cardiomyocyte hypertrophy, interstitial fibrosis, ventricular dilation, contractile dysfunction and / or cell death / apoptosis; - treat, prevent, reduce the risk of, slow down the progression of, delay the onset of, mitigate or reverse renal failure chronic or acute renal and / or peripheral arterial occlusion; - treat, prevent, reduce the risk of, slow down the progression of, delay the onset of, mitigate or reverse congestive heart failure (p for example, NYHA class I, II, III or IV) and / or cardiac hypertrophy (e.g., left ventricular hypertrophy), and / or nephropathic and / or albuminuria; - treating, preventing, reducing the risk of, slowing the progression of, delaying the onset of, attenuating or reversing urenic cardiomyopathy, interstitial expansion and / or cardiac fibrosis (particularly in patients with chronic kidney or heart disease associated with type 2 diabetes); - modulate, block, reduce or protect against the harmful metabolic memory effect of (chronic, early or transient episodes of) hyperglycemia, particularly in diabetic complications; - prevent or protect against oxidation of atherogenic or pro-atherogenic lipoprotein (particularly, low density LDL particles) and / or atherosclerotic plaque formation; - prevent or protect against deficiency induced by oxidative stress in the function or viability of pancreatic beta cells; - treat, prevent, attenuate or decrease the inflammation of pancreatic islets or lipotoxicity and or islet glycotoxicity, or increase the ratio of beta cells to alpha cells, protect beta cells or normalize / improve the morphology or function of pancreatic islets; and / or - preventing, reducing the risk of, slowing the progression of, delaying the onset of, mitigating, reversing or treating diabetes mellitus composition such as micro and macrovascular diseases, such as, nephropathy, micro or macroalbuminuria, proteinuria, retinopathy, cataracts, neuropathy, learning or memory impairment, neurodegenerative or cognitive disorders, cardio- or cerebrovacular diseases, endothelial dysfunction, tissue ischemia, diabetic foot or diabetic ulcer, atherosclerosis, hypertension, myocardial infarction, acute coronary syndrome, angina unstable chest, stable angina, peripheral arterial occlusive disease, cardiomyopathy (including, for example, uremic cardiomyopathy), heart failure, heart rhythm disorders, vascular restenosis, and / or stroke; particularly and independently of or beyond glycemic control; in a patient in need of it (for example, a patient with type 1 diabetes, LADA or, especially, with type 2 diabetes); wherein said methods comprise administering an effective amount of a certain DPP-4 inhibitor, optionally in combination with an amount of one or more other active substances to the patient.
In addition, the present invention relates to a certain DPP-4 inhibitor for use in a method to prevent, reduce the risk of, slow down the progression of, delay the onset of, mitigate, reverse or treat diabetic nephropathy, in a patient (such as such as, for example, a patient as described here, especially type 2 diabetes), who does not respond adequately to therapy with an angiotensin receptor blocker (ARB such as, for example, telmisartan), said method comprising administering a therapeutic amount peutically effective DPP-4 inhibitor, optionally in combination with one or more other therapeutic substances (for example, ARB such as, for example, telmisartan), to the patient.
The characteristics of diabetic nephropathy may include hyperfiltration (at an early stage), micro or macroalbuminuria, nephrotic syndrome, proteinuria, hypertension, fluid retention, edema, and / or renal function or renal filtration that is progressively impaired or decreased (eg, glomerular filtration rate (GFR) ultimately leading to renal failure or end-stage renal disease. Other features may include diffuse or nodular glomerulosclerosis, afferent or efferent hyaline arteriosclerosis, and / or tubulointerstitial fibrosis and atrophy. Other characteristics may include abnormal albumin / creatinine or protein / creatinine ratio and / or abnormal glomerular filtration rate.
The present invention further relates to a certain DPP-4 for use in a method to prevent or treat diabetic nephropathy with an inadequate response to therapy with an angiotensin receptor blocker (ARB, such as, for example, telmisartan). The method may comprise administering a therapeutically effective amount of the DPP-4 inhibitor and telmisartan to the patient.
Therefore, in a particular embodiment, a preferred DPP-4 inhibitor within the meaning of the present invention is linagliptin.
Pharmaceutical compositions or combinations for use in these therapies comprising the DPP-4 inhibitor as defined herein optionally together with one or more other active substances are also contemplated.
In addition, the present invention relates to DPP-4 inhibitors, optionally in combination with one, two or more of other active agents, each as defined herein, for use in the therapies as described here.
In addition, the present invention relates to the use of DPP-4 inhibitors, optionally in combination with one, two or more of other active agents, each as defined herein, to prepare pharmaceutical compositions that are suitable for the treatment and / or for the purpose of preventing this invention.
In addition, the present invention relates to a therapeutic method (treatment or prevention) as described herein, wherein said method comprises administering an effective amount of a DPP-4 inhibitor as described herein, and, optionally, one or more of others active or therapeutic agents, as described here to the patient in need of it. Brief Description of Drawings
Fig. 1 shows the effect of linagliptin on ROS triggered by zymosan A (ZymA) in human PMN (LPS = lipopolysaccharide, PMN = polymophonuclear neutrophils, BI1356 = linagliptin, Nebi = nebivolol).
Fig. 2 shows the effect of linagliptin on the adhesion of human leukocytes (PMN) to human endothelial cells, following stimulation with LPS (Turks and CF-DA staining, B11356 = linagliptin).
Fig. 3 shows the effect of linagliptin on LPS-induced adhesion (50 μg / mL) of neutrophils to EA-hy cells - as measured by the oxidation of Amplex red.
Fig. 4A shows the effect of gliptins on the oxidative explosion in human neutrophils isolated by stimulation with luminescence (LPS = lipopolysaccharide, WBC = white blood cells, LG = BI1356 = linagliptin, AG = alogliptin, VG = vildagliptin, SaG = saxagliptin, SiG = sita-glyiptine, Nebi = nebivolol) intensifies with luminal / strong root peroxidase (HRP).
Fig. 4B shows the effect of gliptins on the oxidative explosion in human monocytes / lymphocytes by stimulation with LPS or zymosan A with chemiluminescence (LPS = liposaccharide, WBC = white blood cells, LG = B11356, AG = alogliptin, VG = vildagliptin, SaG = saxagliptin, SiG = sitagliptin, Nebi = nebivolol) enhanced with luminol / strong root peroxidase (HRP).
Fig. 5 is a table that compares glyptins in direct anti-oxidative effects in vitro.
Fig. 5 shows the effect of linagliptin on oxidation triggered by LPS-activated neutrophils from the peroxidase-derived ROS scavenger and inhibition of NADPH oxidase activity. Quantification of the oxidative explosion in isolated human PMN (5x105 cells / ml) with increasing concentrations of LPS and linagliptin by enhanced chemiluminescence using the luminol analogue L-012 (100pM). (PBS = phosphate buffered saline, LPS = liposaccharide, PMN = polymorphonuclear neutrophils LG = B11356 = linagliptin).
Figs. 7A and 7B show the effect of linagliptin on oxidative burst / oxidative stress of whole blood in nitrate tolerance induced by nitroglycerin (LPS = lipopolysaccharide, EtOH Ctr = ethanol control, GTNs.c. = glyceryl-subcutaneous trinitrate, B11356 = linagliptin).
Fig. 8A and Fig. 8B show the improvement of endothelial dysfunction by linagliptin in rats treated with GTN and LPC (pretreatment with linagliptin (3-10 mg / kg, induction of endothelial dysfunction by nitrates or LPS (3 days) .
Fig. 8A shows the effect of endothelial dysfunction induced with GTN and treatment with linagliptin on endothelium-dependent relaxation EtOH Ctr = ethanol control, GTN s.c. = glyceryl trinitrate - subcutaneous, BI1356 = linagliptin).
Fig. 8B shows the effect of in vivo treatment with LPS (10 mg / kgjd i.p.) and treatment with linagliptin in endothelium-dependent relaxation (LPS = liposaccharide, EtOH Ctr = control).
Fig. 9A and Fig. 9B show the direct vasodilatory effects of glyptins. Gliptin-induced vasodilation is determined by isometric tension recording in isolated aortic ring segments and relaxation in response to increasing cumulative concentrations (1nM to 32μM) of linagliptin, sitagliptin, or saxagliptin (Fig. 9A). In another set of experiments, aortic relaxation in response to increasing cumulative concentrations (1nM to 32 or 100pM) of linagliptin, alogliptin, or vilda-glyiptine is tested (Fig. 9B). Data are mean ± SEM of 12 (Fig. 9A) or 4 (Fig. 9B) rat aortic rings. *, p <0.05 vs. DMSO (solvent control); #, p <0.05 vs. sita- / vildagliptin e§, p <0.05 vs. saxa- / alogliptin.
Fig. 10 shows the blood sugar-based renal function detected after treatment with linagliptin, telmisartan or the combination versus placebo in animals treated with STZ: 1) Non-diabetic eNOS ko control mice, placebo (notrosol) (n = 14 ) 2) eNOS ko diabetic mice treated with placebo, placebo (natrosol) (n = 17) 3) eNOS ko diabetic mice treated with telmisartan (po 1 mg / kg) (n = 17) 4) eNOS ko diabetic mice treated with linagliptin (po 3 mg / kg) (n = 14) 5) eNOS ko diabetic mice treated with telmisartan (1 mg / kg) + linagliptin (3 mg / kg) (n = 12).
Fig. 11 shows the albumin / creatinine ratio of non-diabetic animals vs. diabetics: 1) non-diabetic eNOS ko control mice, placebo (notrosol) (n = 14) 2) placebo-treated eNOS ko mice, placebo (natrosol) (n = 17) 3) diabetic eNOS ko mice treated with telmisartan ( po 1 mg / kg) (n = 17) 4) diabetic eNOS ko mice treated with linagliptin (po 3 mg / kg) (n = 14) 5) diabetic eNOS ko mice treated with telmisartan (1 mg / kg) + Lignagliptin ( 3 mg / kg) (n = 12).
Fig. 12 shows the results of a rat study showing the effects of the combination of telmisartan with linagliptin (BL 1356), and mono-treatment with telmisartan (Telmi solo) or linagliptin (BL 1356 solo) on blood pressure in a model of hypertension-induced cardiac hypertrophy resulting in heart failure. In Fig. 12, between time point 3 and time point 5, the first line from the top refers to the 2K1C placebo (highest systolic RR, the second line from the top refers to linagliptin, the line in the middle it refers to telmisartan, the second line from the bottom refers to placebo, and the first line from the bottom refers to telmisartan + linagliptin (lowest systolic RR).
Fig. 13 is a table showing the results of a mouse model study of chronic renal failure showing the effects of linagliptin on markers of cardiac fibrosis and markers of left ventricular dysfunction in cardiac tissue (TGF-β = beta-factor of transforming growth, TIMP = tissue metalloproteinase inhibitor, Col1a = type 1 alpha collagen, Col3a = type 3a collagen, BNP = type B natriuretic peptide).
Fig. 14 shows the results of a study in diabetic knockout C57BL / 6J mice as a model of diabetic nephropathy that is refractory to ARB treatment showing effects of linagliptin and telmisartan on albuminuria. Detailed Description of the Invention
Oxidative stress represents an imbalance between the production of reactive oxygen species (which include free radicals, which typically have an unpaired electron in oxygen or nitrogen in their outermost orbitals and peroxides) and an ability of the biological system to detoxify intermediates reactive and repair the resulting injury. Disturbance in the normal redox state of tissues can cause effects through the production of peroxides and free radicals that damage all components of the cell, including proteins, lipids and nucleic acid / DNA. Oxidative stress can target many organs (such as blood vessels, eyes, heart, skin, kidneys, joints, lung, brain, immune system, liver and multiple organs) and can be involved in many diseases and conditions. Examples of such diseases or conditions associated with oxidative stress include atherosclerosis (eg, platelet activation and atheromatous platelet formation), endothelial dysfunction, restenosis, hypertension, peripheral occlusive vascular disease, ischemic reperfusion injury (for example, ischemic reperfusion injury) renal, hepatic, cardiac or cerebral), fibrosis (for example, renal, hepatic, cardiac or pulmonary fibrosis); macular degeneration, retinal degeneration, cataracts, retinopathy; coronary heart disease, ischemia, myocardial infarction; psoriasis, dermatitis; chronic kidney disease, nephritis, acute renal failure, glomerulonephritis, nephropathy; rheumatoid atritis, osteoarthritis; asthma, COPD, respiratory distress syndrome; stroke, neurodegenerative diseases (eg, Alzheimer's disease, Parkinson's disease, Huntington's disease), schizophrenia, bipolar disorder, obsessive compulsive disorder; chronic systemic inflammation, perivascular inflammation, autoimmune disorder, multiple sclerosis, irritable bowel disease, ulcerative colitis; NA- FLD / NASH, chronic fatigue syndrome, cystic ovary syndrome, sepsis, diabetes, metabolic syndrome, insulin resistance, hyperglycemia, hypersulinemia, dyslipidemia, hypercholesterolemia, hyperlipidemia, etc. In addition to their original pharmacological properties, certain drugs used clinically, including, without limitation, hypertensive agents, angiotensin receptor blockers and antihyperlipidemic agents such as statins, protect various organs via antioxidative stress mechanisms.
Patients with or at risk for oxidative and / or vascular stress can be diagnosed by determining patient oxidative stress markers, such as oxidized LDL, inflammatory status markers (eg, pro-inflammatory interleukins), 8-OhdG , isoprostans (for example, F2-isoprostanes, 8-isoprostaflandin F2alpha), nitrotyrosine, or N-carboxymethyl lysine (CML).
Endothelial dysfunction, commonly clinically assessed as vasomotion (for example, imbalance between vasodilation and vasoconstriction), is an inability of endothelial cells, the cells that line the inner surface of blood vessels, arteries and veins, which prevents them from performing their functions normal biochemical reactions. Normal endothelial cells are involved in mediating coagulation processes, platelet adhesion, immune function, volume control and electrolyte content in intravascular and extravascular spaces. Endothelial dysfunction is associated with pro-inflammatory, pro-oxidative and pro-thrombotic changes within the arterial wall. Endothelial dysfunction is considered to be a key event in the development and progression of atherosclerosis and arterial stiffness, predating several clinically obvious vascular complications. Endothelial dysfunction is of prognostic significance in the detection of vascular disease and in the prediction of adverse vascular events. Risk factors for atherosclerosis and vascular diseases / events are associated with endothelial dysfunction. Endothelial injury also contributes to the development of kidney damage and / or chronic or progressive kidney damage, such as, for example, tubulointerstitial fibrosis, glomerulonephritis, micro or macroalbuminuria, nephropathy and / or chronic kidney disease or kidney failure. There is evidence to support that oxidative stress not only contributes to endothelial dysfunction or injury but also to vascular disease.
Type 2 diabetes mellitus is a common chronic and progressive disease that arises from a complete pathophysiology that involves the dual endocrine effects of insulin resistance and impaired insulin secretion with the consequence of not meeting the requirements to maintain plasma glucose levels. in the normal range, this leads to hyperglycemia and its associated micro and macrovascular complications or chronic lesions, such as, for example, nephropathy, retinopathy or diabetic neuropathy, or macro vascular complications (for example, cardiovascular or cerebrovascular). The vascular disease component plays a significant role, but it is not the only factor in the spectrum of disorders associated with diabetes. The high frequency of complications leads to a significant reduction in life expectancy. Diabetes is currently the most frequent cause of the onset of loss of vision in adults, and amputation in the industrialized world because of complications induced by diabetes and is associated with an increase of two to five in the risk of cardiovascular disease.
Large randomized studies have established that intensive and strict glycemic control during early-stage diabetes (recent diagnoses for 5 years) has supported beneficial effects and reduces the risk of diabetic complications, both micro and macrovascular. However, many patients with diabetes still develop diabetic complications in spite of receiving intensified glycemic control.
Epidemiological and prospective data support a long-term influence of early metabolic control (recently diagnosed for 5 years) on clinical outcomes. It has been found that hyperglycemia has long-lasting deleterious effects in both type 1 and type 2 diabetes and that glycemic control, if not initiated at a very early stage of the disease or not intensively or not rigidly provided, may not be sufficient to completely reduce complications. It was also found that transient episodes of hyperglycemia (for example, hyperglycemic events) can induce molecular changes, and that these changes may persist or are irreversible after returning to normoglycemia.
Collectively, these data suggest that metabolic memories are stored early in the course of diabetes and that, under certain diabetic conditions, oxidative and / or vascular stresses may persist after glucose normalization. This phenomenon, which in the early glycemic environment and / or even in transient hyperglycemia, is remembered with clinical consequences in the target terminal organs (for example, blood vessels, retina, kidneys, heart, extremities), has recently been referred to as "metabolic memory".
Potential mechanisms to propagate this "memory" are certain epigenetic alterations, the glycation of cellular proteins and lipids (for example, formation of advanced glycation end products), oxidatively modified atherogenic lipoproteins, and / or excess of reactive oxygen species and cellular species nitrogen (RONS), in particular originating from the level of glycated mitochondrial proteins, perhaps acting in accordance with another to maintain the signaling of stress.
Mitochondria are one of the main sources of reactive oxygen species (ROS) in cells. Mitochondrial dysfunction increases electron leakage and the generation of ROS from the mitochondrial respiratory chain (MRC). High levels of glucose and lipid compromise the activities of complex MRC enzymes. For example, the MRC enzyme NADPH oxidase generates superoxide from NADPH in cells. The increased activity of NADPH oxidase can be detected in diabetic patients.
Still, there is evidence that overproduction of free radicals, such as, for example, reactive oxygen species (ROS), contributes to oxidative and vascular stress after glucose normalization and to the development and maintenance of metabolic memory, and so on. for the unifying link between the effects of hyperglycemia and cellular memory, such as, for example, on endothelial dysfunction and other complications of diabetes.
Thus, mainly related to persistent (long-term) oxidative stress induced by or associated with hyperglycemia (chronic, early or transient episodes), there are certain metabolic conditions in which, even normalizing blood glucose, a long-term persistent activation of many pathways involved in pathogenesis of diabetic complications may still be present. One of the main findings in the course of diabetes here has been the demonstration of even in normoglycemia and regardless of actual glycemic levels an overproduction of free radicals may still be evident. For example, endothelial dysfunction (a causative marker of diabetic complications) can persist even after glycemia normalizes. However, there is evidence that the combination of anti-oxidant therapy and normalization of blood glucose can be used to almost stop endothelial dysfunction.
Therefore, the treatment of oxidative and / or vascular stress particularly beyond glycemic control, such as by reducing reactive cell species and / or glycation (for example, by inhibiting the production of oxygen and nitrogen free radicals), preferable and regardless of glycemic status, it can beneficially modulate, reduce, block or protect the memory effect of hyperglycemia and reduce the risk, prevent, treat or delay the onset of long-term diabetic complications, particularly those that are associated or are induced by oxidative stress in patients who need it.
Treatment of type 2 diabetes typically begins with diet and exercise, followed by oral antidiabetic monotherapy, and although conventional monotherapy can initially control blood glucose in some patients, it is nevertheless associated with a high rate of insufficiency secondary. The limitations of single agent therapy to maintain glycemic control can be overcome, at least in some patients and for a limited period of time by combining multiple drugs to achieve reductions in blood glucose that cannot be sustained during long-term therapy. with single agents. The available data support the conclusion that in most patients with type 2 diabetes, current monotherapy will fail and treatment with multiple drugs will be required. However, due to the fact that type 2 diabetes is a progressive disease, even patients with good initial responses to conventional combination therapy will eventually require an increase in dosage or other insulin treatment because the blood glucose level is very difficult to maintain. keep stable for a long period of temp. As existing combination therapy has the potential to enhance glycemic control, it is not without limitations (especially with regard to long-term efficacy). Still, traditional therapies can show an increased risk of side effects, such as hypoglycemia or weight gain, which can compromise their effectiveness and acceptability.
Thus, for many patients, these existing drug therapies result in progressive deterioration in metabolic control despite treatment and do not sufficiently control metabolic status especially for a long term and thus fail to achieve and maintain glycemic control in advanced type 2 diabetes. or late, including diabetes with inadequate glycemic control despite medication, conventional, oral or non-oral antidiabetic.
Therefore, although intensive treatment of hyperglycemia can reduce the incidence of chronic lesions, many patients with type 2 diabetes remain inadequately treated, partly because of the limitations of long-term efficacy, tolerability and inconvenience in the dosage of conventional anti-hyperglycemic therapies.
This high incidence of therapeutic failure is what mainly contributes to the high rate of complications associated with long-term hyperglycemia or chronic injuries (including micro and macrovascular complications, such as, for example, nephropathy, retinopathy or diabetic neuropathy, or complications brain- or cardiovascular, such as, for example, myocardial infarction, stroke or mortality or vascular morbidity (in patients with type 2 diabetes).
Oral antidiabetic drugs used conventionally in therapy (such as, for example, first or second line and / or mono (or initial or added) combination therapy include, without being restricted to any theory, metformin, sulfonylureas, thiazolidinediones, glinides, and α-glycosidase inhibitors.
Antidiabetic drugs used conventionally in therapy (such as, for example, first or second line and / or mono (or initial or added) combination therapy) include, but are not limited to, GLP-1 or GLP-1 analogs, and insulin and insulin analogues.
However, the use of these conventional anti-diabetic or anti-hyperglycemic agents can be associated with several adverse effects. For example, metformin may be associated with lactic acidosis or gastrointestinal side effects; sulfonylureas, glinides and insulin or insulin analogues may be associated with hypoglycemia and weight gain; thiazolidinediones may be associated with edema, bone fracture, weight gain and heart failure / cardiac effects; and alpha-glucosidase and GLP-1 blockers or GLP-1 analogs may be associated with adverse gastrointestinal effects (eg, dyspepsia, flatulence or diarrhea, or nausea or vomiting), and, more seriously (but rare), pancreatitis.
Therefore, there is still a need in the art to provide effective, safe and tolerable antidiabetic therapies.
Still, within the therapy of type 2 diabetes, there is a need to treat the condition effectively, avoiding the complications inherent in the condition, and slowing the progression of the disease, for example, to obtain long-term therapeutic benefit.
In addition, there is a need for antidiabetic treatments that not only prevent the long-term complications often encountered in advanced stages of diabetes disease, but are also a therapeutic option for patients who have developed or are at risk for diabetes. develop complications, such as kidney failure.
In addition, there is still a need to provide prevention or risk reduction for adverse effects associated with conventional antidiabetic therapies.
The DPP-4 enzyme (dipeptidyl peptidase IV) also known as CD26 is a serine protease known to lead to the cleavage of a peptide from the N-terminal end of several proteins having at its N-terminal end a proline or alanine residue. Due to these properties, DPP-4 inhibitors interfere with the plasma level of bioactive peptides including the GLP-1 peptide and are considered to be promising drugs for the treatment of diabetes mellitus.
For example, DPP-4 inhibitors and their uses are described in WO 2002/068420, WO 2004/018467, WO 2004/018468, WO 2004/018469, WO 2004/041820, WO 2004/046148, WO 2005/051950, WO 2005/082906, WO 2005/063750, WO 2005/085246, WO 2006/027204, WO 2006/029769, W02007 / 014886; WO 2004/050658, WO 2004/111051, WO 2005/058901, WO 2005/097798; WO 2006/068163, WO 2007/071738, WO 2008/017670; WO 2007/128721, WO 2007/128724, WO 2007/128761, or WO 2009/121945.
In monitoring the treatment of diabetes mellitus, the value of HbA1c, the product of a non-enzymatic glycation of the hemoglobin B chain, is of exceptional importance. Since its formation depends essentially on the blood sugar level and the lifespan of the erythrocytes, HbA1c in the sense of a "blood sugar memory" reflects the average blood sugar level of the previous 4 to 12 weeks. Diabetic patients, whose HbA1 level has been well controlled for a long time by more intensive treatment of diabetes (i.e. <6.5% of the total hemoglobin in the sample) are significantly better protected against diabetic micro-angiopathy. Diabetes treatments can give the diabetic an average improvement in their HbA1c level in the order of 1.0 - 1.5 ”. This reduction in the level of HbA1C is not sufficient in all diabetics to place it in the desired target range of <7.0%, preferably <6.5% and more preferably <6% HbA1c.
Within the meaning of this invention, inadequate or insufficient glycemic control means in particular a condition in which patients show HbA1c values above 6.5%, in particular above 7.0%, even more preferably above 7.5% , especially above 8%. A modality of patients with inadequate or insufficient glycemic control includes, but is not limited to, patients having an HbA1c value of 7.5 to 10% (or, in another modality, 7.5 to 11%). A special sub-modality of inadequately controlled patients refers to patients with poor glycemic control including, without limitation, patients having an HbA 1c value> 9%.
Within glycemic control, in addition to improving the level of HbA1c, other recommended therapeutic targets for patients with type 2 diabetes mellitus are the improvement of fasting plasma glucose (FPG) and postprandial plasma glucose levels (PPG) and normal or as almost as normal as possible. Recommended target ranges for preprandial plasma glucose (fasting) are 70-130 mg / dL (or 90-130 mg / dL) or <110 mg / dL, and two hour postprandial plasma glucose is <180 mg / dL or <140 mg / dL.
In one embodiment, patients with diabetes within the meaning of this invention may include patients who have previously been treated with an antidiabetic drug (patients (naive) who have never been treated with this drug before). Thus, in one embodiment, the therapies described here can be used in naive patients. In another embodiment, patients with diabetes within the meaning of this invention may include patients with advanced or late type 2 diabetes mellitus (including patients with failed therapy) conventional antidiabetic), such as, for example, patients with inadequate glycemic control on one, two or more conventional oral and / or non-oral drugs as defined here, such as, for example, patient with insufficient glycemic control despite (mono) therapy with metformin, a thiazolidinedione (particularly pioglitazone), a sulfonylurea, a glinide, GLP-1 and GP-1 analogue, insulin or insulin analogue, or an α-glycosidase inhibitor, or despite dual combination therapy with metformin / sulfonylurea, metformin / thiazolidinedione (particularly pioglitazone), sulphonylurea / glucosidase inhibitor, pioglitazone / sulphonylurea, mephromine / insulin, pioglite zo- / insulin or sulfonylurea / insulin. Thus, in one modality, the therapies described here can be used in patients who have undergone therapy, for example, with medication in combination mono, or dual, or triple, antidiabetic, conventional oral and / or non-oral, as mentioned here.
Another modality of diabetic patients within the meaning of this invention refers to patients ineligible for meftormin therapy, including - patients for whom metformin therapy is contraindicated, for example, patients having one or more contraindications against metformin therapy. according to the label, such as, for example, patients with at least one selected contraindication for: kidney disease, kidney deficiency or kidney dysfunction (for example, as specified by the locally approved metformin product information), dehydration, congestive heart failure unstable or acute, acute or chronic metabolic acidosis, and hereditary galactose intolerance, and - patients who suffer from one or more intolerable side effects attributed to metformin, particularly metformin-associated gastrointestinal side effects, such as, for example, patients suffering from at least one gastrointestinal side effect selected in tre: nausea, vomiting, diarrhea, intestinal gas, and severe abdominal discomfort.
Another modality of patients with diabetes, who may be receptive to the therapies of this invention may include, without limitation, those patients with diabetes for whom normal metformin therapy is not appropriate, such as, for example, those patients with diabetes who need therapy with metformin in dose received due to reduced tolerability, intolerability or contraindication to metformin or due to renal function or due to (slightly) impaired / reduced renal function (including elderly patients, such as those with age, for- example,> 60-65 years).
Another modality of diabetic patients within the meaning of the invention refers to patients having kidney disease, kidney dysfunction, or impaired or impaired kidney function (including mild, moderate and severe kidney deficiency), for example, as suggested by elevated serum creatinine levels. (eg, serum creatinine levels above the upper limit of normal for their age, for example, £ 130 - 150 μmol / L, or only 1.5 mg / dL (£ 136 pmol / dL) in men and £ 1 , 4 mg / dL (£ 124 μmol / L) in women) or abnormal creatinine clearance (eg glomerular filtration rate (GFR) <30-60 mL / min).
In this context, for a more detailed example, mild renal impairment can, for example, be suggested by creatinine clearance of 50-80 mL / min (approximately corresponding to serum creatine levels of £ 1.7 mg / dL in men and <1.5 mg / dL in women); moderate renal impairment can be, for example, suggested by creatinine clearance of 30-50 ml / min (corresponding approximately to serum creatinine levels of> 1.7 to £ 3.0 mg / dL in men> 1.5 to £ 2.5 mg / dL women); and severe renal deficiency can, for example, be suggested by creatinine clearance of <30 mL / min (corresponding approximately to serum creatinine levels of> 3.0 mg / dL in men and> 2.5 mg / dL in women). Patients with kidney disease in the last stage require dialysis (for example, hemodialysis or peritoneal dialysis).
For another more detailed example, patients with kidney disease, kidney dysfunction or disability include patients with chronic kidney failure or impairment, which can be stratified to the glomerular filtration rate (GFR, mL / min / 1.73 m2) in 5 stages disease: stage 1 characterized by normal GFR> 90, more or persistent albuminuria or known or hereditary structural kidney disease; stage 2 characterized by a mild reduction in GFR (GFR 60-89) describing mild renal impairment; stage 3 characterized by a moderate reduction in GFR (GFR 30-59) describing moderate renal deficiency; stage 4 characterized by severe GFR reduction (GFR 15-29) describing severe renal impairment; and terminal stage 5 characterized by requiring dialysis or GFR <15, describing stabilized renal failure (end stage renal disease, ESRD).
Another modality of diabetic patients within the meaning of this invention relates to patients with type 2 diabetes mellitus with or at risk of developing micro or macrovascular diabetic complications, such as, for example, described here (for example, such patients at risk as described in follow).
Another modality of diabetic patients within the meaning of this invention refers to patients with type 2 diabetes with or at risk of developing renal complications, such as diabetic nephropathy (including chronic and progressive renal failure, albuminuria, proteinuria, fluid retention in the body (edema) and / or hypertension.
Another modality of patients with diabetes, who may be receptive to the therapies of this invention, may include, without limitation, those with or at risk of developing complications in the retina, such as diabetic retinopathy.
Another modality of patients with diabetes, who may be receptive to the therapies of this invention, may include, without limitation, those patients with type 2 diabetes with or at risk of developing macrovascular complications, such as myocardial infarction, coronary artery disease, stroke cerebral ischemic or hemorrhagic, and / or peripheral arterial occlusive disease.
Another modality of diabetic patients, who may be receptive to the therapies of this invention, may include patients with type 2 diabetes with or at risk for cardio- or cerebrovascular diseases or events (such as, for example, those at cardiovascular risk described here) .
Another modality of diabetic patients, who may be receptive to the therapies of this invention, may include, without limitation, diabetic patients (specifically type 2 diabetes) with advanced age and / or with advanced diabetes disease, such as, for example, patients being treated with insulin, patients with triple oral antidiabetic therapy, patients with pre-existing cardio- and / or cerebrovascular events with duration of advanced disease (for example,> / = 5 to 10 years).
Another modality of diabetic patients who may be receptive to the therapies of the invention may include, without limitation, those diabetic patients (especially type 2 diabetic patients) with one or more cardiovascular risk factors selected from A), B) C) and D) ; A) previous or existing vascular disease (such as, for example, myocardial infarction (for example, silent or non-silent), coronary artery disease, percutaneous coronary intervention, myocardial revascularization, ischemic or hemorrhagic stroke, congestive heart failure ( for example, NYHA class I or II, for example, left ventricular function <40%), or peripheral occlusive arterial disease (B) related target organ damage, vascular (such as, for example, nephropathy, retinopathy, neuropathy , impaired kidney function, chronic kidney disease, and / or micro or macroalbuminuria), C) advanced age (such as, for example, age> / = 60-70 years), and D) one or more cardiovascular risk factors selected from - advanced type 2 diabetes mellitus (such as, for example,> 10 years in duration), - hypertension (such as, for example,> 130/80 mmHg, or systolic blood pressure> 140 mmHg or at least a treatment that lowers blood pressure uine), - current daily cigarette smoker, - dyslipidemia (such as, for example, atherogenic dyslipidemia, postprandial lipemia, or high level of LDL cholesterol (eg cholesterol> / = 130-135 mf / dL), low level of HDL cholesterol (eg <35-40 mg / dL in men or <45-50 mg / dL in women) and / or high triglycerides (eg> 200-400 mg / dL) in the blood , or at least one treatment for abnormal lipids), - obesity (such as, for example, abdominal and / or visceral obesity, or body mass index> / = 45 kg / m2). - age ./= 40 and </ = 80 years, - metabolic syndrome, hyperinsulinemia or insulin resistance, and - hyperuricemia, erectile dysfunction, polycystic ovary syndrome, sleep apnea, or family history of vascular disease or cardiomyopathy in a relative of first degree.
In certain embodiments, patients who may be receptive to the therapies of this invention may have or be at risk for one or more of the following diseases, disorders or conditions: type 1 diabetes, type 2 diabetes, impaired glucose tolerance (IGT), glucose in the compromised fasting blood (IFG), hyperglycemia, postprandial hyperglycemia, post-absorptive hyperglycemia, latent autoimmune diabetes in adults (LADA), overweight, obesity, dyslipidemia (including, for example, atherogenic dyslipidemia), hyperlipidemia, hypercholesterolemia, hypertriglycerid hyperNEFA-emia, postprandial lipemia, hypertension, atherosclerosis, endothelial dysfunction, osteoporosis, chronic systemic inflammation, non-alcoholic fatty liver disease (NAFLD), polycystic ovarian syndrome, hyperuricemia, metabolic syndrome, nephropathy, micro or macroalbuminuria, proteinuria , retinopathy, cataracts, neuropathy, learning or memory deficit, neurodegenerative or cognitive disorders, cataracts, cardiovascular or cerebrovascular diseases, tissue ischemic, diabetic foot or diabetic ulcer, atherosclerosis, hypertension, endothelial dysfunction, myocardial infarction, acute coronary syndrome, unstable angina pectoris, stable angina pectoris, peripheral arterial occlusive disease, cardiomyopathy (including , for example, uremic cardiomyopathy), heart failure, cardiac hypertrophy, cardiac rhythm, vascular restenosis, stroke, ischemic / reperfusion injuries (renal, cardiac, brain or liver), fibrosis (renal, cardiac, cerebral or hepatic ), vascular remodeling (renal, cardiac, cerebral or hepatic; diabetic disease, especially type 2 diabetes, diabetes mellitus being preferred (for example, as an underlying disease).
In another embodiment, patients who may be receptive to the therapies of this invention have diabetic disease, especially type 2 diabetes mellitus, and may have or be at risk for one or more other diseases, disorders or conditions, such as, for example, selected of those mentioned above.
Within the scope of the present invention, it has now been found that certain DPPP-4 inhibitors, as defined herein, optionally in combination with one or more therapeutic substances (for example, selected from those described here), as well as pharmaceutical combinations, compositions or combined uses according to this invention such DPP-4 inhibitors defined herein have properties that can make them suitable for the purpose of the present invention and / or to satisfy one or more of the above needs.
The present invention further relates to a certain DPP-4 inhibitor, as defined herein, preferably linagliptin (BI 1356), for use in the therapies described herein.
The present invention further relates to a certain DPP-4 inhibitor, as defined herein, preferably linagliptin (BI 1356), in combination with metformin, for use in the therapies described herein.
The present invention further relates to a certain DPP-4 inhibitor, as defined herein, preferably linagliptin (BI 1356), in combination with pioglitazone, for use in the therapies described herein.
The present invention further relates to a certain DPP-4 inhibitor, as defined herein, preferably linagliptin (BI 1356), in combination with telmisartan, for use in the therapies described herein.
The present invention relates to a pharmaceutical composition comprising a certain DPP-4 inhibitor, as defined herein, preferably linagliptin (BI 1356), for use in the therapies described herein.
The present invention relates to a pharmaceutical composition that comprises a certain DPP-4 inhibitor, as defined herein, preferably linagliptin (BI 1356), and metformin, for use in the therapies described herein.
The present invention relates to a pharmaceutical composition that comprises a certain DPP-4 inhibitor, as defined herein, preferably linagliptin (BI 1356), and pioglitazone, for use in the therapies described herein.
The present invention also relates to a combination comprising a certain DPP-4 inhibitor (particularly BI 1356) and one or more active substances selected from those mentioned here, for example, other anti-diabetic substances, active substances that decrease the level of sugar in the blood, active substances that lower the blood lipid level, active substances that increase the level of HDL in the blood, active substances that reduce blood pressure, active substances that are indicated in the treatment of atherosclerosis or obesity, antiplatelet agents, agents anticoagulants, and vascular endothelial protective agents, for example, each as described here, particularly for simultaneous, separate or sequential use in the therapies described here.
The present invention also relates to a combination comprising a certain DDP-4 inhibitor (particularly BI 1356) and one or more other antidiabetic products selected from the group consisting of metformin, sulfonylurea, nateglinide, repaglidine, thiazolidinedione, PPAR- agonist. gamma, alpha-glycosidase inhibitor, insulin or insulin analog, and GLP-1 or GLP-1 analog, particularly for separate or sequential use in the therapies described herein, optionally in combination with telmisartan.
The present invention further relates to a method for treating and / or preventing metabolic diseases, especially type 2 diabetes mellitus and / or conditions related to it (e.g., diabetic complications) comprising combined administration (e.g., simultaneous, separate or sequential) an effective amount of one or more other antidiabetic products selected from the group consisting of metformin, sulfonylurea, nateglinide, repaglidine, thiazolidinedione, PPAR-gamma agonist, alpha-glucosidase inhibitor, insulin or insulin analogue, and GLP-1 or GLP-1 analogue, and an effective amount of DPP-4 inhibitor (particularly B11356) as defined herein, and, optionally an effective amount of telmisartan, to the patient (particularly human patient) in need thereof, such as , for example, a patient as described here, including patients at risk.
The present invention further relates to therapies or therapeutic methods described herein, such as, for example, a method for treating and / or preventing metabolic diseases, especially type 2 diabetes mellitus and / or conditions related to it (for example, diabetic complications ) comprising administering a therapeutically effective amount of linagliptin (BI 1356) and, optionally, one or more of other thiazolidinedione agents, PPAR-gamma agonist, alpha-glucosidase inhibitor, insulin or insulin analogue, and GLP-1 or GLP-1 analogue, and / or telmisartan, to the patient (particularly human patient) in need of it, such as, for example, a patient as described here (for example, patient at risk as described here).
The present invention further relates to therapies or therapeutic methods described herein, such as, for example, a method for treating and / or preventing metabolic diseases, especially type 2 diabetes mellitus and / or conditions related to it (for example, diabetic complications) including the administration of a therapeutically effective amount of linagliptin (BI 1356) to the patient (particularly a human patient) in need thereof, such as, for example, a patient as described here, including a patient at risk ( particularly a patient such as or at risk for cardio- or cerebrovascular diseases or events and / or with or at risk for kidney disease) as described herein.
The present invention further relates to therapies or therapeutic methods described herein, such as, for example, a method for treating and / or preventing metabolic diseases, especially type 2 diabetes mellitus and / or conditions related to it (for example, diabetic complications) including the administration of a therapeutically effective amount of linagliptin (BI 1356) and metformin to the patient (particularly human patient) in need of it, such as, for example, a patient as described here, including a patient at risk ( particularly a patient such as or at risk for cardio- or cerebrovascular diseases or events) as described herein.
The present invention further relates to therapies or therapeutic methods described herein, such as, for example, a method for treating and / or preventing metabolic diseases, especially type 2 diabetes mellitus and / or conditions related to it (for example, diabetic complications) including the administration of a therapeutically effective amount of linagliptin (BI 1356) to the patient (particularly human patient) telmi-sartan to the patient (particularly human patient in need of it, such as, for example, a patient as described here, including patient at risk (particularly a patient such as or at risk for cardio- or cerebrovascular diseases or events and / or with or at risk for kidney disease) as described here.
Examples of such metabolic disorders or diseases receptive to the therapy of this invention, (particularly in patients having or at risk for cardiovascular and / or kidney disease, may include, without limitation, type 1 diabetes, type 2 diabetes, impaired glucose tolerance (IGT), impaired fasting blood glucose (IFG), hyperglycemia, postprandial hyperglycemia, post-absorptive hyperglycemia, latent autoimmune diabetes in adults (LADA), overweight, obesity, dyslipidemia, hyperlipidemia, hypercholesterolemia, hypertriglyceridemia, hyperNE -emia, postprandial lipemia, hypertension, atherosclerosis, endothelial dysfunction, osteoporosis, chronic systemic inflammation, non-alcoholic fatty liver disease (NAFLD), retinopathy, neuropathy, nephropathy, polycystic ovary syndrome, and / or meta-bolic syndrome.
The present invention also relates to the following methods: - preventing, slowing the progression of, slowing down or treating a metabolic disorder or disease, such as, for example, type 1 diabetes mellitus, type 2 diabetes mellitus, impaired glucose tolerance (IGT) , impaired fasting blood glucose (IFG), hyperglycemia, postprandial hyperglycemia, post-absorptive hyperglycemia, latent autoimmune diabetes in adults (LADA), overweight, obesity, dyslipidemia, hyperlipidemia, hypercholesterolemia, hypertriglyceridemia, hyperNEIG-emia, hyperNEFA-emia postprandial lipemia, hypertension, atherosclerosis, endothelial dysfunction, osteoporosis, chronic systemic inflammation, non-alcoholic fatty liver disease (NAFLD), retinopathy, neuropathy, nephropathy, polycystic ovary syndrome, and / or meta-balloon syndrome; - improve and / or maintain glycemic control and / or to reduce fasting plasma glucose, postprandial plasma glucose, post-absorptive plasma glucose and / or glycosylated hemoglobin HbA1c; - prevent, slow, delay or reverse the progression of pre-diabetes, impaired glucose tolerance (IGT), impaired fasting blood glucose (IFG), insulin resistance and / or metabolic syndrome for type 2 diabetes mellitus; - prevent, reduce the risk of, slow down the progression of, delay or treat complications of diabetes mellitus such as micro and macrovascular diseases, such as nephropathy, micro or macroalbuminuria, proteinuria, retinopathy, cataracts, neuropathy, learning deficit and of memory, neurodegenerative or cognitive disorders, cardiovascular diseases, tissue ischemia, diabetic foot or diabetic ulcer, atherosclerosis, hypertension, endothelial dysfunction, myocardial infarction, acute coronary syndrome, unstable angina pectoris, stable angina pectoris, peripheral arterial occlusive disease, cardiomyopathy (including, for example, uremic cardiomyopathy), heart failure, heart rhythm disturbances, vascular restenosis and / or stroke; - reduce body weight and / or body fat or prevent an increase in body weight and / or body fat or facilitate a reduction in body weight and / or body fat; - prevent, slow down, delay or treat the degeneration of pancreatic beta cells and / or the decline in the functionality of pancreatic beta cells and / or improve, preserve and / or restore the functionality of pancreatic beta cells and / or stimulate and / or restore or protect pancreatic insulin secretion functionality; - prevent, slow down, delay and or treat non-alcoholic fatty liver disease (NAFLD) including liver steatosis, non-alcoholic steatohepatitis (NASH) and / or liver fibrosis (such as, for example, preventing, slowing down progression, slowing down, alleviate, treat or reverse liver steatosis, (hepatic) inflammation and / or abnormal accumulation of fat in the liver; - prevent, slow down progression, delay or treat failed type 2 diabetes for mono or combination antidiabetic therapy, conventional ; - obtain a reduction in the dose of conventional antidiabetic medication required for adequate therapeutic effect; - reduce the risk of adverse effects associated with conventional antidiabetic medication (eg, hypoglycemia and / or weight gain); and / or - maintain and / or improve insulin sensitivity and / or treat or prevent hyperinsulinemia and / or insulin resistance; in a patient who needs it (such as, for example, a human patient as described here, especially a patient with type 2 diabetes), particularly in a patient with or at risk of oxidative stress, vascular stress and / or endothelial dysfunction or related or associated diseases or conditions, or in a patient or at risk for cardiovascular disease and / or renal (such as, for example, myocardial infarction, stroke or peripheral arterial occlusive disease and / or diabetic nephropathy, micro or macroalbuminuria, or acute or chronic renal failure), or in a patient with one or more risk factors cardiovascular diseases selected from A), B), C), and D): A) previous or existing vascular disease (such as, for example, myocardial infarction (eg, silent or non-silent), coronary artery disease, intervention percutaneous coronary artery, myocardial revascularization, ischemic or hemorrhagic stroke, congestive heart failure (eg NYHA class I or II, eg left ventricular function <40%), or peripheral arterial occlusive disease), B) vascular, related target organ damage (such as, for example, nephropathy, retinopathy, neuropathy, impaired kidney function, chronic kidney disease, and / or micro or macroalbuminuria), C ) advanced age (such as, for example, age> / = 60-70 years), and D) one or more cardiovascular risk factors selected from - advanced type 2 diabetes mellitus (such as, for example,> 10 years), - hypertension (such as, for example,> 130/80 mmHg, or systolic blood pressure> 140 mmHg or in at least one treatment that lowers blood pressure), - current daily cigarette smoker, - dyslipidemia (such as such as atherogenic dyslipidemia, postprandial lipemia, or high level of LDL cholesterol (for example, cholesterol> / = 130-135 mf / dL), low level of HDL cholesterol (for example, <35-40 mg / dL in men or <45-50 mg / dL in women) and / or high triglycerides (eg> 200-400 mg / dL) in the blood, or at least s a treatment for lipid abnormality), - obesity (such as, for example, abdominal and / or visceral obesity, or body mass index> / = 45 kg / m2). - age ./= 40 and </ = 80 years, - metabolic syndrome, hyperinsulinemia or insulin resistance, and - hyperuricemia, erectile dysfunction, polycystic ovary syndrome, sleep apnea, or family history of vascular disease or cardiomyopathy in a patient with first degree; wherein said method comprises administering a therapeutically effective amount of a certain DPP-4 inhibitor, optionally in combination with one or more other therapeutic substances as described herein.
Other aspects of the present invention will become apparent to those skilled in the art from the comments above and below (including the examples and claims).
Aspects of the present invention, in particular pharmaceutical compounds, compositions, combinations, methods and uses, relate to DPP-4 inhibitors as defined above and below.
A DPP-4 inhibitor within the meaning of the present invention includes, without limitation, any of those DPP-4 inhibitors mentioned above and below, preferably orally active DPP-4 inhibitors.
One embodiment of this invention relates to a DPP-4 inhibitor for use in the treatment and / or prevention of metabolic diseases (particularly type 2 diabetes mellitus) in patients with type 2 diabetes, in which said patients still suffer from kidney disease. , renal dysfunction or renal deficiency, particularly characterized by the fact that said DPP-4 inhibitor is administered to said patients at the same dose levels as to patients with normal renal function, thus, for example, said DPP-4 inhibitor no dose adjustment is required for impaired renal function.
For example, a DPP-4 inhibitor according to this invention (especially one that may be suitable for patients with impaired renal function) can be such as an oral DPP-4 inhibitor, which and whose active metabolites preferably have a relatively wide therapeutic window (for example, about> 100 times) and / or, especially, which are mainly eliminated via hepatic metabolism or by biliary excretion (preferably without adding another load to the kidneys).
In a more detailed example, a DPP-4 inhibitor according to this invention (especially one that may be suitable for patients with impaired kidney function) may be an orally administered DPP-4 inhibitor, which has a relatively broad therapeutic window. (for example,> 100-fold) (preferably, a safety profile comparable to placebo) and / or that satisfies one or more of the following pharmacokinetic properties (preferably at its therapeutic dose levels): - The DPP- inhibitor 4 is substantially or mainly excreted via the liver (for example,> 80% or even> 90% of the administered oral dose), and / or for which renal excretion does not represent any route of elimination or only a small elimination (for example, < 10%, preferably <7% of the oral dose administered, for example, following the elimination of an oral dose of a radiolabelled carbon substance (14C); - The DPP-4 inhibitor is mainly excreted mainly unchanged as a drug the original (for example, with an average of> 70%, or> 80%, or, preferably, 90% of radioactivity excreted in urine and faeces after oral dosing of the radiolabeled substance (14C), and / or that is eliminated at a substantial extent or only a minor extent via metabolism (for example, <30%, or <20%, or, preferably, 10%). - The metabolism (s) of the DPP-4 inhibitor is (are) pharmacologically active (s). As, for example, the metabolite does not primarily bind to the target enzyme DPP-4 and, optionally, it is rapidly eliminated compared to the parent compound (for example, with a terminal half-life of the metabolite of <20 h, or, preferably, <about 16 hours, such as, for example, 15.9 h).
In one embodiment, the plasma (main) metabolite (which may be pharmacologically inactive) of a DPP-4 inhibitor having a 3-amino-piperidin-1-yl substituent is such a derivative in which the amino group of fraction 3- amino-piperidin-1-yl is replaced by a hydroxyl group to form the 3-hydroxy-piperidin-1-yl fraction (for example, the 3- (S) -hydroxy-piperidin-1-yl fraction, which is formed by inversion of the chiral center configuration).
Other properties of a DPP-4 inhibitor according to this invention can be one or more of the following: Rapidly obtaining a constant state (for example, achieving steady state plasma levels (> 90% of the steady state plasma concentration) ) between the second and the fifth day of treatment with oral therapeutic dose levels), little accumulation (for example, with an average RAAUC accumulation ratio £ 1.4 with oral therapeutic dose levels), and / or maintaining a long-lasting effect on DPP-4 inhibition, preferably when used once daily (for example, with almost complete (> 90%) inhibition of DPP-4 at oral therapeutic dose levels),> 80% inhibition over a range 24 hours after ingestion once a day of> 80% (already on the first day of therapy) at therapeutic dose levels, and the cumulative amount of the original compound unchanged excreted in the urine on the first day of below 1% of administered dose and increasing to no the more than about 3-6% in constant state.
Thus, for example, a DPP-4 inhibitor according to this invention can be characterized by the fact that said DPP-4 inhibitor has a mainly non-renal pathway of excretion, ie said DPP-4 inhibitor is excreted at a non-substantial or only small extent (for example, <10%, preferably <7%, for example, about 5%, of the administered oral dose, preferably of the therapeutic oral dose) via the kidneys (measured, for example, by following the elimination of an oral dose of the radiolabelled carbon substance (14C)).
In addition, a DPP-4 inhibitor, according to this invention, can be characterized by the fact that the DPP-4 inhibitor is excreted substantially or mainly via the liver and feces (whose measurement is done, for example, example, following the elimination of an oral dose of the radiolabelled carbon substance (14C).
Furthermore, a DPP-4 inhibitor, according to this invention, can be characterized by the fact that said DPP4 inhibitor is excreted mainly unchanged as the original drug (for example, with an average of> 70%, or> 80 %, or, preferably, 90% of the radioactivity excreted in the urine and faeces after oral dosing of the radiolabelled carbon substance (14C), said DPP-4 inhibitor is eliminated to a non-substantial or only minor extent via metabolism, and / or the main metabolite of said DPP-4 inhibitor is pharmacologically inactive or has a relatively wide therapeutic window.
Furthermore, a DPP-4 inhibitor, according to this invention, can be characterized by the fact that said DPP-4 inhibitor does not significantly compromise the glomerular and / or tubular function of the patient with type 2 diabetes with chronic renal failure ( for example, mild, moderate or severe kidney deficiency or end-stage kidney disease), and / or said DPP-4 inhibitor through blood plasma levels of type 2 diabetes patients with mild or moderate kidney deficiency are comparable to levels in patients with normal renal function, and / or said DPP-4 inhibitor does not require an adjusted dose in a patient with type-2 diabetes with impaired renal function (for example, mild, moderate or severe renal impairment or disease kidney in the final stage, preferably, regardless of the stage of renal deficiency).
Furthermore, a DPP-4 inhibitor, according to this invention, can be characterized by the fact that said DPP-4 inhibitor provides its minimally effective dose at that dose which results in> 50% inhibition of DPP-4 activity. at the lowest level (24 h after the last dosage) in> 0% of patients, and / or said DPP-4 inhibitor provides its total therapeutic dose at that dose which results in> 80% inhibition of DPP-4 activity at the lowest level (24 hours after the last dose) in> 80% of patients.
Furthermore, a DPP-4 inhibitor, according to the invention, can be characterized by the fact that it is suitable for use in patients with type 2 diabetes who are diagnosed with renal impairment and / or who are at risk of developing renal complications. , for example, patients with or at risk of diabetic nephropathy (including chronic and progressive renal failure, albuminuria, proteinuria, fluid retention in the body (edema and / or hypertension).
In a first embodiment (modality A), a DPP-4 inhibitor in the context of the present invention is any DPP-4 inhibitor of formula (i)
or formula (ii)
or formula (III)
or of formula (IV) V
wherein R1 represents ([1,5] naphthyridin-2-yl) methyl, (quinazolin-2-yl) methyl, (quinoxalin-6-yl) methyl, (4-methyl-quinazolin-2-yl) methyl , 2-cyano-benzyl, (3-cyano-quinolin-2-yl) methyl, (3-cyano-pyridin-2-yl) methyl, (4-methyl-pyrimidin-2-yl) methyl, or (4, 6-dimethyl-pyrimidin-2-yl) methyl and R2 represents 3 - (/ ) - amino-piperidin-1-yl, (2-amino-2-methyl-propyl) -methylamino or (2- (S) - amino-propyl) -methylamino, or its pharmaceutically acceptable salt.
With respect to the first modality (modality A), preferred DPP-4 inhibitors are any or all of the following compounds and their pharmaceutically acceptable salts: • 1 - [(4-methyl-quinazolin-2-yl) meti] -3-metill -7- (2-butin-1-yl) -8- (3- (R) -amino-piperidin-1-yl) -xanthine (compare WO 2004/018468, Example 2 (142)):
• 1 - [([1,5] naphthyridin-2-yl) methyl] -3-methyl-7- (2-butin-1-yl) -8 - ((R) -3- amino-piperidin-1- il) -xanthine (compare WO 2004/018468, Example 2 (252)):
• 1 - [(Quinazolin-2-yl) methyl] -3-methyleth-7- (2-butyn-1 -yl) -8 - ((/ ) - 3-amino-piperidin-1-yl) -xanthine (compare WO 2004/018468, example 2 (80)):
• 2 - ((R) -3-Amino-pipθridin-1-yl) -3- (b ut-2-yl) -5- (4-methyl-quinazolin-2-ylmethyl) -3,5-di -hydro-imidazo [4,5-d] pyridazin-4-one (compare WO 2004/050658, Example 136):
• 1 - [(4-Methyl-quinazolin-2-yl) methyl] -3-methyl-7- (2-butynin-1-yl) -8 - [(2-amino-2-methyl-propyl) -methylamino ] -xanthine (compare WO 2006/029769, E-10 xample2 (1)):
• 1 - [(3-Cyano-quinolin-2-yl) methyl] -3-methyl-7- (2-butin-1-yl) -8 - ((/ ) - 3-amino-piperidin-1- il) -xanthine (compare WO 2005/085246, Example 1 (30)):
* 1 - (2-Cyano-benzyl) -3-methyl-7- (2-butin-1-yl) -8 - ((R) -3-amino-piperidin-1-yl) -xanthine (compare WO 2005 / 085246, Example 1 (39)):
• 1 - [(4-Methyl-inazolin-2-yl) methyl] -3-methyl-7- (2-butin-1-yl) -8 - [(S) - (2-amino-propyl ) -methylamino] -xanthine (compare WO 2006/029769, Example 2 (4)):
• 1 - [(4-Methyl-quinazolin-2-yl) methyl] -3-methyl-7- (2-butin-1-yl) -8 - [(S) - (2-amino-propyl) -methylamino ] -xanthine (compare WO 2006/029769, Example 2 (4)):
• 1 - [(3-Cyano-pyridin-2-yl) methyl] -3-methyl-7- (2-butin-1-yl) -8 - ((/ ) - 3- amino-pipθridin-1- il) -xanthine (compare WO 2005/085246, Example 1 (52)):
• 1 - [(4-Methyl-pyrimidin-2-yl) methyl] -3-methyl-7- (2-buti-1-yl) -8 - ((/ ) - 3-amino- piperidin-1-yl) -xanthine (compare WO 2005/085246, Example 1 (81)):
• 1 - [(4,6-Dimetikpirimidin-2-yl) methyl] -3-methyl-7- (2-butin-1-yl) -8 - ((/ ) - 3-amino-piperidin-1- il) -xanthine (compare WO 2005/085246, Example 1 (82)):
• 1 - [(Quinoxalin-6-yl) methyl] -3-methyl-7- (2-butin-1-yl) -8 - ((R) -3-amino-piperidin-1-yl) -xanthine ( compare WO 2005/085246, Example 1 (83)): O

These DPP-4 inhibitors are distinguished from structurally comparable inhibitors in that they combine exceptional potency and a long-lasting effect with favorable pharmacological properties, receptor selectivity and a favorable side effect profile or have unexpected therapeutic benefits or improvements when combined with other active pharmaceutical substances. Its preparation is described in the publications mentioned above.
A more preferred DPP-4 inhibitor among the DPP-4 inhibitors mentioned above of modality A of this invention is 1 - [(4-methyl-quinazolin-2-yl) methyl] -3-methyl-7- (2- butin-1-yl) -8- (3 - (/ ) - amino-piperidin-1-yl) - xanthine, particularly its free base (which is still known as linagliptin or B11356).
A particularly preferred inhibitor of DPP-4 within the present invention is linagliptin. The term "linagliptin", as used herein, refers to linagliptin or a pharmaceutically acceptable salt thereof, including its hydrates and solvates, and its crystalline forms, preferably, linagliptin refers to 1 - [(4-methyl-quinazolin-2 -yl) methyl] -3-methyl-7- (2-butin-1-yl) -8- (3- (/ ) - amino-piperidin-1-yl) -xanthine. Crystalline forms are described in WO 2007/128721. Methods for making linagliptin are described in Patent Applications WO 2004/018468 and WO 2006/048427, for example. Linagliptin is distinguished from structurally comparable DPP-4 inhibitors in that it combines exceptional potency and a long-lasting effect with favorable pharmacological properties, receptor selectivity and a favorable side effect profile or have unexpected therapeutic advantages or improvements in therapy mono or dual or triple combination.
For the avoidance of doubt, the description of the documents cited above and below in connection with the specified DPP-4 inhibitors is specifically incorporated here by way of reference in its entirety;
Within the scope of this invention, it is to be understood that combinations, compositions or combined uses according to the present invention may contemplate the simultaneous, sequential or separate administration of the active ingredients or components.
In that context, "combination" or "combined" according to the meaning of this invention, may include, without limitation, fixed or non-fixed forms (for example, free) (including kits) and uses, such as, for example, use simultaneous, sequential or separate from the components or ingredients.
The combined administration of this invention can be carried out by administering the active ingredients or components together, such as, for example, by administering them simultaneously in single formulations or in two separate formulations or dosage forms. Alternatively, administration can be carried out by administering the active ingredients or components sequentially, such as, for example, in two separate formulations or dosage forms.
For the combination therapy of this invention, the components or active ingredients can be administered separately (which implies that they are formulated separately) or formulated together (which implies that they are formulated in the same preparation or in the same dosage form), Logo , the administration of one element of the combination of the present invention can be prior, concurrent, or subsequent to the administration of the other element of the combination.
Unless otherwise combined, combination therapy may refer to first-line, second-line or third-line therapy or add-on therapy or replacement therapy.
With respect to modality A, the synthesis methods for DPP-4 inhibitors, according to modality A of this invention, are known to those skilled in the art. Advantageously, DPP-4 inhibitors, according to embodiment A of this invention, can be prepared using synthetic methods as described in the literature. Thus, for example, the purine derivatives of formula (I) can be obtained as described in WO 2002/068420, WO 2004/018468, WO 2005/085246, WO 2006/029769 or WO 2006/048427, the content of which is embedded here. The purine derivatives of formula (II) can be obtained as described, for example, in WO 2004/050658 or WO 2005/110999, the disclosure of which is incorporated herein. Purine derivatives of formulas (III) or (IV) can be obtained, as described, for example, in WO 2006/068163, WO 2007/071738 or WO 2008/017670, the content of which is incorporated herein. The preparation of those DPP-4 inhibitors, which are specifically mentioned, is described in the publications mentioned here in connection with it. Modifications to polymorphic crystals and formulations of particular DPP-4 inhibitors are described in WO 2007/128721 and WO 2007/128724, respectively, the content of which is incorporated herein in full. Formulations of particular DPP-4 inhibitors with metformin or other combination partners are described in WO 2009/121945, the disclosure of which is incorporated herein in its entirety.
Typical dosage strengths of the dual fixed (tablet) combination of linagliptin / metformin IR (immediate release) are 2.5 / 500 mg, 2.5 / 850 mg and 2.5 / 1.00 mg, which can be administered 1-3 times a day, particularly twice a day.
Typical dosage strengths of the dual fixed (tablet) combination of linagliptin / metformin XR (extended release) are 5/500 mg, 5 / 1,000 mg and 5 / 1,500 (each tablet), or 2.5 / 500mg, 2.6 / 750 mg and 2.5 / 1,000 mg (each two tablets), which can be administered 1 to 2 times a day, particularly once a day, preferably taken at night with a meal.
The present invention further provides a DPP-4 inhibitor as defined herein for use in therapy in combination (with addition or initial) with metformin (for example, in a total daily amount of 500 to 2,000 mg of metformin hydrochloride, such as, for example, 500 mg, 850 mg or 1,000 mg once or twice a day.
For pharmaceutical application in warm-blooded vertebrates, particularly humans, the compounds of this invention are commonly used in dosages from 0.001 to 100 mg / kg of body weight, preferably at 0.01-15 mg / kg or 0.1-15 mg / kg. kg, in each case, 1 to 4 times while still. For this purpose, the compounds, optionally combined with other active substances, can be incorporated together with one or more conventional inert vehicles and / or diluents, for example, with starch, lactose, glucose, microcrystalline cellulose, magnesium stearate, pyrrolidone polyvinyl lidine, citric acid, tartaric acid, water, water / ethanol, water / glycerol, water / sorbitol, water / polyethylene glycol, propylene glycol, cetylstearyl alcohol, carboxymethylcellulose or fatty substances such as hard fat or suitable mixtures of these in galenical preparations conventional tablets such as plain or coated tablets, capsules, powders, suspensions or suppositories.
The pharmaceutical compositions according to this invention, comprising DPP-4 inhibitors, as defined herein, are thus prepared by one skilled in the art using excipients of pharmaceutically acceptable formulations. Examples of such excipients include, without limitation, diluents, binders, vehicles, fillers, lubricants, fluidity promoters, crystallization retardants, disintegrators, solubilizers, dyes, pH regulators, surfactants and emulsifiers.
The oral preparations or dosage forms of the DPP-4 inhibitor of this invention can be prepared according to known techniques.
Examples of suitable diluents for compounds according to modality A include cellulose powder, calcium hydrogen phosphate, erythritol, low substituted hydroxypropyl cellulose, mannitol, pregelatinized starch or xylitol.
Examples of suitable lubricants for compounds according to modality A include talc, polyethylene glycol, calcium beenate, calcium stearate, hydrogenated castor oil or magnesium stearate.
Examples of suitable binders for compounds according to modality A include copovidone (vinylpyrrolidone copolymerized with other vinyl derivatives), hydroxypropyl methylcellulose (HPMC), hydroxypropyl cellulose (HPC), polyvinyl pyrrolidone (povidone), pregelatinized starch or hydroxopropylcellulose low substitution (L-HPC).
Examples of suitable disintegrants for compounds according to modality A include corn starch or crospovidone.
Suitable methods for preparing the pharmaceutical formulations of DPP-4 inhibitors according to embodiment A of the invention are: • direct tabletting of the appropriate substance in powder mixtures with tablet-forming excipients; • granulation with suitable excipients and subsequent mixing with suitable excipients and subsequent formation of the tablets as a film coating; or • packaging of powder or granule mixtures in capsules. Suitable granulation methods are • wet granulation in an intensive mixer followed by dry- • granulation in a simple pan; • fluidized bed granulation; • dry granulation (example powder, compaction with laminator) with suitable excipients and subsequent formation of tablets or capsule packaging.
Exemplary composition (e.g., tablet core) of a DPP-4 inhibitor according to embodiment A of the invention comprises the first diluent mannitol, pregelatinized starch as a second diluent with additional binder properties, the copovidone binder, the corn starch disintegrator, and magnesium stearate as a lubricant; wherein copovidone and / or corn starch may be optional. A DPP-4 inhibitor tablet according to embodiment A of the invention can be a coated film, preferably the coated film comprises hydroxypropylmethyl cellulose (HPMC), polyethylene glycol (PEG), talc, titanium dioxide and iron oxide ( for example, red and / or yellow).
Pharmaceutical compositions (or formulations) can be packaged in many ways. Generally, an article for distribution includes one or more containers that contain one or more pharmaceutical compositions in an appropriate manner. The tablets are typically packaged in a suitable main packaging for ease of handling, distribution and storage and to ensure proper stability of the composition in prolonged contact with the environment during packaging. The main containers for tablets can be bottles or blister packs.
A bottle suitable, for example, for a pharmaceutical composition or combination (tablet) comprising a DPP-4 inhibitor, according to embodiment A of the invention, can be made of glass or polymer (preferably polypropylene (PP) or high density polyethylene (HD-PE) and sealed with a screw cap The screw cap can be provided with a child resistant safety closure (eg pressure and twist closure) to prevent or lock access to the contents If required (for example, in regions with high humidity), by the additional use of desiccant (such as bentonite clay, or, preferably silica gel), the shelf life of the packaged composition can be extended.
A blister pack suitable, for example, for a pharmaceutical composition or combination (tablet) comprising a DPP-4 inhibitor according to embodiment A of the invention, comprises or is formed of a top sheet (which is tear-away by tablets) and a bottom part (containing blisters for the tablets). The top sheet may contain a metal sheet, particularly an aluminum or aluminum alloy sheet (for example, having a thickness of 20 μm to 45 μm, preferably from 20 μm to 25 μm) which is coated with a layer of thermo polymer -sealed on its internal side (side of the seal). The bottom part may contain a multilayer polymer sheet (such as, for example, poly (vinyl chloride) (PNV) with poly (vinylidene chloride) (PVDC); or a PVC sheet laminated with poly (chlorotrifluoroethylene) (PCT-FE) or a polymer-metal-polymer multilayer sheet (such as, for example, a laminated, thermo-conformable PVC / aluminum / polyamide composition;
To ensure a long storage period under hot and humid climatic conditions, an additional cover or pouch made of the polymer-metal-polymer multilayer sheet (eg a laminated polyethylene / aluminum / polyester composition) can be used for packaging blister. Supplemental desiccant (such as, for example, bentonite clay, molecular sieves, or, preferably, silica gel) in such a pouch can further extend the shelf life under such stringent conditions.
The article may also comprise a label or package insert, which contains instructions usually contained in commercial packaging for therapeutic products, which may contain information on the indications, use, dosage, administration, contraindications and / or warnings regarding the use of such therapeutic products. In one embodiment, the label or package insert indicates that the composition can be used for any of the products described here.
With respect to the first modality (modality A), the typically required dosage of DPP-4 inhibitors mentioned here in modality A when administered intravenously is 0.1 mg to 10 mg, preferably 0.25 to 5 mg, and when administered orally it is from 0.5 to 100 mg, preferably 2.5 mg to 50 mg or 0.5 mg to 10 mg, more preferably 2.5 mg to 10 mg or 1 mg to 5 mg, in each case from 1 to 4 times a day. Thus, for example, the dosage of 1 - [(4-methyl-quinazolin-2-yl) methyl] -3-methyl-7- (2-butin-1-yl) -8- (3 - (/ ) -amino-piperidin-1-yl) -xanthine, when administered orally, is 0.5 mg to 10 mg per patient per day, preferably 2.5 mg to 10 mg or 1 mg to 5 mg per patient per day.
A dosage form prepared with a pharmaceutical composition, which comprises a DPP-4 inhibitor mentioned here in modality A, contains the active ingredient in a dosage range ranging from 0.1 to 100 mg. Thus, for example, particular oral dosages of 1 - [(4-methyl-quinazolin-2-yl) methyl] -3-methyl-7- (2-butin-1-yl) -8- (3 - (/ ) -amino-piperidin-1-yl) -xanthine are 0.5 mg, 1 mg, 2.5 mg, 5 mg and 10 mg.
A special modality of the DPP-4 inhibitors of this invention relates to those orally administered DPP-4 inhibitors, which are therapeutically effective at low dose levels, for example, dose levels of <100 mg or <70 mg can patient per day , preferably <50 mg, more preferably <30 mg or <20 mg, even more preferably from 1 mg to 10 mg, particularly from 1 mg to 5 mg (more particularly 5 mg), per patient per day (if required, divided into 1 to 4 single doses, particularly 1 or 2 single doses, which can be of the same size, preferably administered orally once or twice a day (more preferably once a day), advantageously administered at any time of the day, with or So, for example, the daily oral amount of 5 mg of BI 1356 can be given in a once-daily dosing regimen (ie, 5 mg of BI 1356 once a day) or in a dosing regimen twice a day (ie 2.5 mg BI 1356 twice a day) day), at any time of the day, with or without food.
The dosage of the active ingredients in the combinations and compositions according to the present invention can be varied, although the amount of the active ingredients is such that a suitable dosage form is obtained. Therefore, the selected dosage or the selected dosage form will depend on the desired therapeutic effect, the route of administration and the duration of treatment. Dosage ranges suitable for the combination are from the maximum tolerated dose for the single agent to the lowest doses, for example, to 1/10 of the maximum tolerated dose.
A particularly preferred inhibitor to be emphasized with the meaning of this invention is 1 - [(4-methyl-quinazolin-2-yl) methyl] -3-methyl-7- (2-butin-1-yl) -8- ( 3- (R) -amino-piperidin-1-yl) -xanthine (also known as BI 1356 or linagliptin). BI 1356 exhibits high potency, 24 duration of action, and a wide therapeutic window. In patients with type 2 diabetes who are receiving multiple oral doses of 1, 2.5, 5 or 10 mg of BI 1356 once daily for 12 days, BI 1356 shows a favorable pharmacodynamic and pharmacokinetic profile (see, for example, Table 1 below) with rapid attainment of steady state (for example, reaching steady plasma levels (> 90% of plasma concentration on Day 13) between the second and fifth day of treatment in all dose groups), little accumulation (for example, with an average RA accumulation ratio, AUC 1.4 with doses above 1 mg) and maintaining a long-lasting effect on the inhibition of DPP-4 (for example, with almost complete inhibition (> 90%) of DPP-4 at the dose levels of 5 mg and 10 mg, ie, 92.3 and 97.3% inhibition in the constant state, respectively, and> 80% inhibition during a 24-hour interval after drug ingestion ), as well as a significant decrease in 2 h postprandial blood glucose excursions of> 80% (already on Day 1) at doses> 2.5 mg , and the cumulative amount of the original unchanged compound being excreted in the urine on Day 1 of 1% of the administered dose and increasing to no more than about 3-6% on Day 12 (CLR renal clearance, SS is about 14 at about 70 ml / min for the oral doses administered, for example, for renal clearance the dose of 5 mg is about 60 ml / min). In patients with type 2 diabetes, BI 1356 shows safety and tolerance similar to placebo. With low doses of about> 5mg, BI 1356 acts as a real oral drug once a day for a total duration of 24 hours and only to a lesser extent (about <7% of the administered oral dose) via the kidneys. BI 1356 is mainly excreted unchanged via the bile. The fraction of BI 1356 eliminated via the kidneys increases only very slightly over time and with increasing dose, so there is likely to be no need to modify the BI 1356 dose based on the patient's renal function. The non-renal elimination of BI 1356 in combination with its low accumulation potential and wide safety margin can be of significant benefit in a patient population that has a high prevalence of renal failure and diabetic nephropathy. Table 1: Geometric mean (gMean) and geometric coefficient of variation (gCV) of the pharmacokinetic parameters of BI 1356 in constant state (Day 12)
Average and range [min-max] NC not calculated as most values below the lower limit of quantification
Since different metabolic functional disorders often occur simultaneously, it is very often indicated to combine several different active ingredients with one another. Thus, depending on the functional disorders diagnosed, improved treatment results can be obtained if a DPP-4 inhibitor is combined with active substances customary for the respective disorders, such as, for example, one or more active substances selected from among other diabetic substances, especially active substances that reduce blood sugar or blood lipid levels, raise blood levels of HDL, lower blood pressure or are indicated for the treatment of atherosclerosis or obesity.
The DPP-4 inhibitors mentioned above - in addition to their use in monotherapy - can also be used in conjunction with other active substances, by means of which improved treatment can be obtained. Such a combined treatment can be given as a free combination of substances or in the form of a fixed combination, for example, in a tablet or capsule. The pharmaceutical formulations of the combination partner for this can either be obtained commercially as pharmaceutical compositions or can be formulated by one skilled in the art using conventional methods. The active substances that can be obtained commercially as pharmaceutical compositions are described in various places in the prior art, for example, in the list of drugs that appears annually, the "Rote Liste®" of the federal association of the pharmaceutical industry, or in the compilation updated annually of information to manufacturers on prescription drugs known as the "Physicians's Desk Reference".
Examples of partners for diabetic combinations are metformin; sulfonylureas, such as glibenclamide, tolbutamide, glimepiride, glipizide, gluquidone, glubomuride and glycazide; nateglinide; repaglinide; mitiglycinide; thiazolidinediones such as rosiglitazone and pioglitazone; PPAR-gamma modulators such as metaglidases; PPAR-gamma agonists such as, for example, rivoglitazone, mitoglitazone, INT-131 and balaglitazone; PPAR-gamma antagonists; PPAR-gamma / ala modulators such as thesaglitazar, muraglitazar, aleglitazar, indeglitazar and KRP297; PPAR-gamma / alpha / delta modulators such as, for example, lobeglitazone, AMPK activators such as AICAR; acetyl-CoA carboxylase inhibitors (ACC1 and ACC2); diacylglycerol-acetyltranferase (DGAT) inhibitors; pancreatic beta cell GCRP agonists such as SMT3 and GPR119 receptor agonists, such as GPR119 agonists 5-ethyl-2- {4- [4- (4-tetrazol-1-yl-phenoxymethyl) -thiazole-2- yl] -piperidi n-1 -yl} -pi rimidene or 5- [1 - (3-isopropyl- [1,2,4] oxadiazol-5-yl) -piperidin-4-ylmethoxy-2- (4 -methanesulfonyl-pheni) -pyridine; 11β-HSD inhibitors; FGF19 agonists or analogs; alpha-glycosidase blockers such as acarbose, voglibose and miglitol; alpha2 antagonists; insulin and insulin analogues such as human insulin, lispro insulin, glusilin insulin, r-DNA-insulinsparte, NPH insulin, insulin determinal, degludec insulin, tregopyl insulin, zinc insulin suspension and insulin glargine; Gastric Inhibitor Peptide (GIP); amylin and amylin analogues (for example, pranlintide or davalintide); GLP-1 and GLP-1 analogs such as Exendin-4, for example, exentatide, exenatide LAR, liraglutide, taspoglutide, lixisenatide (AVE-0010), LY-2428757 (a Pegylated version of GLP-1), dulaglutide (L Y-2189265), semagglutide or albiglutide; SGLT2 inhibitors such as, for example, dapaglifozin, serglifozin (KGT-1251), atigliflozin, cannagliflozin, ipragliflozin or tofogliflozin; protein tyrosine phosphatase inhibitors (for example, trodusquemine); glucose-6-phosphatase inhibitors; fructose-1,6-bisphosphatase modulators; glycogen modulators phosphorylates; glucagon receptor antagonists; phosphoenolpyruva tercarboxyl kinase (PEPCK) inhibitors; pyruvate dehydrogenase kinase (PDK); tyrosine kinase inhibitors (50 mg to 600 mg) such as PDGF-kinase receptor (see EP-A-564409, WO 98/35958, US 5093330, WO 2004/005281, and WO 2006/041976) or serine / threonine kinases; glucokinase / regulatory protein modulators, including glucokinase activators; glycogen synthase kinase inhibitors; inhibitors of the SH2 domain containing inositol 5-phosphatase type 2 (SHIP2); IKK inhibitors such as high dose salicylate; JNK1 inhibitors; protein kinase C-theta inhibitors; beta 3 agonists such as ritobegron, YM 178, solabegron, talibegron, N-5984, GRC-1087, rafabegron, FMP825; aldosereductase inhibitors such as AS 3201, zenarate, fidarestate, epalrestate, ranirate, NZ-314, CP-744809, and CT-112 inhibitors; SGLT-1 or SGLT-2; KV channel inhibitors 1.3; modulators of GPR40 such as, for example, [(3S) -6 - ({2 ', 6'-dimethyl-4' - [3- (methylsulfonyl) propoxy] biphenyl-3-yl} methoxy) -2,3 dihydro-1-benzofuran-3-iljacetic; SCD-1 inhibitors; CCR-2 antagonists; dopamine receptor agonists (bromocriptine mesylate [Cycloset]; 4- (3- (2,6-dimethylbenzyloxy) phenyl) -4-oxobutanoic acid; sirtuin stimulants; and other DPP-IV inhibitors.
Metformin is usually given in doses ranging from about 500 mg to 2.00o mg to 2.500 mg per day using various dosage regimens from about 100 mg to 500 mg or 200 mg to 850 mg (1 to 3 times a day ), or about 300 mg to 1,000 mg once or twice daily, or delayed-release metformin in doses of about 100 mg to 1.00 mg or preferably 500 mg to 1,000 once or twice a day or about 500 mg to 2,000 once a day. The particular dosage potencies can be 250, 500, 625, 750, 850 and 1,000 mg of metformin hydrochloride.
For children 10 to 16 years of age, the recommended starting dose of metformin is 500 mg is administered once daily. If that dose fails to produce adequate results, the dose can be increased to 500 mg once a day. Other increases can be made in increments of 500 mg weekly for a maximum daily dose of 2,000 mg, given in divided doses (for example, 2 or 3 divided doses). Metformin can be administered with food to increase nausea.
A dosage of pioglitazone is usually about 1 to 10 mg, 15 mg, 30 mg, or 45 mg once a day.
Rosiglitazone is usually given in doses of 4 to 8 mg once a day (or divided into two times) a day (typical dosage potencies are 2, 4 and 8 mg).
Glibenclamide (glyburide) is usually given in doses of 2.5-5 to 20 mg once a day (or divided twice) a day (typical dosage strengths of 1.25, 2.5 and 5 mg), or micronized glibenclamide in doses of 0.75-3 to 12 mg once a day (or divided twice) a day (typical dosage potencies are 1.5, 3, 4.5 and 6 mg).
Glipizide is usually given at a dose of 2.5 to 10-20 mg once (or up to 40 mg divided twice) a day (typical dosage potencies are 5 and 10 mg), or prolonged release glibenclamide in doses of 5 to 10 mg (up to 20 mg) once a day (typical dosage potencies are 2.5, 5 and 10 mg).
Glimepiride is usually given in doses of 1-2 to 4 mg (up to 8 mg) once a day (typical dosage potencies are 1, 2 and 4 mg).
A dual combination of glibenclamide / metformin is usually given in doses of 1.25 / 250 once daily at 10 / 1,000 mg twice daily (typical dosage potencies are 1.25 / 250, 2.5 / 500 and 5/500 mg).
A dual composition of glipizide / metformin is usually given in doses of 2.5 / 250 to 10 / 1,000 mg twice daily (typical dosage potencies are 2.5 / 250, 2.5 / 500 and 5/500 mg ).
A dual combination of glimepiride / metformin is usually given in doses of 1/250 to 4 / 1,000 mg twice daily.
A dual combination of rosiglitazone / glimepiride is usually given in doses of 4/1 once a day at 4/2 mg twice a day (typical dosage potencies are 4/1, 4/2, 8/2 and 8/4 mg). A dual combination of pioglitazone / glimepiride is usually given in doses of 30/2 to 30/4 mg once a day (typical dosage strengths are 30/4 to 45/4 mg).
A dual rosiglitazone / metformin combination is usually given in doses of 1/500, 2/500, 2 / 1,000 and 4 / 1,000 mg).
A dual composition of pioglitazone / metformin is usually given in doses of 15/500 once or twice a day at 15/850 three times a day (typical dosage potencies are 15/500 and 15/850 mg).
The nateglinide non-sulfoniltureia insulin secretagogue is usually given in doses of 60 to 120 mg with meals (up to 360 mg / day, typical dosage potencies are 60 and 120 mg); repaglinide is usually given in doses of 0.5 to 4 mg with meals (up to 16 mg / day, typical dosage potencies are 0.5, 1 and 2 mg). A dual repaglinate / metformin combination is available in dosage strengths of 1/500 and 2/850 mg.
Acarbose is usually given in doses of 25 to 100 mg with meals. Miglitol is usually given in doses of 25 to 100 mg with meals.
Examples of combination partners that reduce the blood lipid level are HMG-CoA reductase inhibitors such as simvastatin, atorvastatin, lovastatin, fluvastatin, pravastatin, pitavastatin and rosavastatin; fibrates such as bezafibrate, fenofibrate, clofibrate, genfibrozil, etofibrate and etophyllinclofibrate; nicotinic acid and its derivatives such as acipimox; PPAR-alpha agonists; PPAR-delta agonists such as, for example, [4 - [(R) -2-ethoxy-3- (4-trifluormethyl-phenoxy) -propylsulfanyl] -2-methyl-phenoxy} -acetic acid inhibitor, acylcoenzyme A. cholesterolacyltransferase (ACAT) inhibitors; EC 2.3 1.26) such as avasimib; cholesterol reabsorption inhibitors such as ezetimibe; substances that bind to bile acid, such as cholestyramine, colestipol and colesevelam; bile acid transport inhibitors; HDL modulating active substances such as D4f, reverse D4F, LXR modulating active substances and FXR modulating active substances; inhibitors of CETP such as torcetrapib, JTT-705 (dealcetrapib) or compound 12 of WO 2007/005572 (a-nacetrapib) or avecetrapib; LDL receptor modulators; MTP inhibitors (for example, lomitapide); and ApoBlOO antisense RNA.
A dose of atorvastatin is usually 1 mg to 40 mg or 10 mg to 80 mg once daily;
Examples of combination partners that lower blood pressure are beta-blockers such as atenolol, bisoprolol, celiprolol, metoprolol, nebivolol and carvedilol; diuretics such as hydrochlorothiazide, chlortalidone, xipamide, furosemide, piretanide, torasemide, spironolactone, eplerenone, amiloride and triamterene; calcium channel blockers such as amlodipine, nifedipine, nitrendipine, nisoldipine, nicardipine, felodipine, lacidipine, lercanipidine, manidipine, isradipine, nilvadipine, verapamil, cockpamil and diltiazem; ACE inhibitors such as ramipril, lisinopril, cilazapril, quinapril, captopril, enalapril, benazepril, perindopril, fosinopril and trandolapril; as well as angiotensin II receptor blockers (ARBs) such as telmisartan, candesartan, valsartan, losartan, irbesartan, olmesartan, azilsartan and eprosartan.
A dosage of telmisartan is usually 20 mg to 320 mg or 40 mg to 160 mg per day.
Examples of combination partners that increase the level of HDL in the blood are inhibitors of Transfer Protein Cholesteryl Esters (CETP); endothelial lipase inhibitors; ABC1 regulators; LXRalfa antagonists; LXRbeta agonists; PPAR-delta agonists; regulators of LXRalpha / beta, and substances that increase the expression and / or plasma concentration of apoliprotein A-l.
Examples of combination partners for the treatment of obesity are sibutramine; tetrahydrolipstatin (orlistat); alizime (cetilistat); dexfenfluramine; axoquine; cannabinoid receptor 1 antagonists such as the CB1 antagonist rimonobant; MCH-1 receptor antagonists; MC4 receptor agonists; NPY5 as well as NPY2 antagonists (for example, velneperite); beta3-AR agonists such as SB-418790 and AD-9677; 5HT2c receptor agonists such as APD 356 (lorecerin); myostatin inhibitors; Acrp30 and adiponectin; styroyl CoA desaturase (SDC1) inhibitors; fatty acid synthase (FAZ) inhibitors; CCK receptor agonists; modulators of Ghrelin receptors; Pyy 3-36; orexin receptor antagonists; and tesofensina; as well as the dual combinations of bupropion / naltrexone, bupropion / zonisamide, topi- ramate / phentermine and pramlintide / metreleptin.
Examples of combination partners for the treatment of atherosclerosis are phospholipase A2 inhibitors; tyrosine kinase inhibitors (50 mg to 600 mg) such as PDGF-receptor kinase (see EP-A-564409, WO 98/35958, US 5093330, WO 2004/005281, and WO 2006/041976); oxLDL antibodies, and oxLDL vaccines; apoA-1 Milano; WING; and VCAM-1 inhibitors.
Furthermore, furthermore, optionally within the meaning of this invention, the DPP-4 inhibitor can be combined with one or more other antioxidants, anti-inflammatories and / or endothelial protective agents.
Examples of antioxidant combination partners are selenium, betaine, vitamin C, vitamin E and beta-carotene.
An example of a partner for a combination of anti-inflammatories is pentoxifylline; another example of an anti-inflammatory combination partner is a PDE-4 inhibitor, such as, for example, tetomilast, roflumilast, or 3 [7-ethyl-2- (methoxymethyl) -4- (5- methyl-3-pyridinyl) pyrrole [1,2-b] pyridazin-3-yl] propanoic (or other species described in US 7153854, WO 2004/063197, US 7459451 and / or WO 2006/004188).
Another example of an anti-inflammatory partner drug is a caspase inhibitor, such as, for example, (3S) -5-fluorine-3 - ({[((5R) - 5-isopropyl-3- (1- isoquinolinyl) -4,5-dihydro-5-isoxazolyl} amino-4-oxapentanoic (or other species described in WO 2005/021515 and / or WO 2006/090997).
An example of a vascular endothelial protective agent is a PDE-5 inhibitor, such as, for example, silfenafil, vardenafil or tadalafil; another example of an endothelial protective agent is a nitric oxide donor or stimulator (such as, for example, L-arginine or tetrahydrobiopterine).
Furthermore, in addition, optionally within the meaning of this invention, the DPP-4 inhibitor can be combined with one or more antiplatelet agents, such as, for example, aspirin (low dose) (acetylsalicylic acid), an inhibitor of selective COX-2 or non-selective COX-1 / COX-2, or an ADP receptor inhibitor, such as a thienopyridine (for example, clopidogrel or prasugrel), elinogrel or ticagrelor, or a thrombin receptor antagonist such as vorapaxar.
In addition, furthermore, optionally within the meaning of this invention, the DPP-4 inhibitor can be combined with one or more anticoagulant agents, such as, for example, heparin, warfarin, or a direct thrombin inhibitor (such as, for example, dabigatran), or a Factor Xa inhibitor (such as, for example, rivaroxaban or apixaban or edoxaban or otamizaban).
Furthermore, in addition, optionally within the meaning of this invention, the DPP-4 inhibitor can be combined with one or more agents for the treatment of heart failure.
Examples of combination partners for the treatment of heart failure are beta-blockers such as atenolol, bisoprolol, celiprolol, metoprolol, nebivolol and carvedilol; diuretics such as hydrochlorotiazide, chlortalidone, xipamide, furosemide, piretanide, torasemide, spironolactone, eplerenone, amiloride and triamterene; ACE inhibitors such as ramipril, lisinopril, cilazapril, quinapril, captopril, enalapril, benazepril, perindopril, fosinopril and trandolapril; as well as angiotensin II receptor blockers (ARBs) such as telmisartan, candesartan, valsartan, losartan, irbesartan, olmesartan, azilsartan and eprosartan; cardiac glycosides such as digoxin and digitoxin; combined alpha / beta-blockers such as carvedilol; vasodilators; antirhythmic drugs; or type B natriuretic peptide (BNP), and peptides derived from BNP and BNP fusion products.
In addition, further, optionally within the meaning of this invention, a DPP-4 inhibitor can be combined with one or more CCK2 or gastrin agonists, such as, for example, proton pump inhibitors (including well-reversible inhibitors). as irreversible from gastric H + / K + -ATPase), for example, omeprazole, esomeprazole, pantoprazole, rabeprazole or lansoprazole.
The present invention is not limited in scope by the specific modalities described here. Various modifications of the invention, in addition to those described here, may become apparent to those skilled in the art from the exposure of the present invention. Such modifications are intended to fall within the scope of the appended claims.
All patent applications are hereby incorporated by reference in their entirety.
Other embodiments, characteristics and advantages of the present invention will become apparent from the following examples. The following examples serve to illustrate, by way of example, the principles of the invention without restriction. EXAMPLES Antioxidant effects: Linagliptin anti-inflammatory and vasodilator potential
Direct antioxidant effects of glyptines (linagliptin, alogliptin, vildagliptin, saxagliptin, sitagliptin) are evaluated by inference with superoxide formation from xanthine oxidase, peroxynitrite (authentic and derived from Sin-1) or 1-electron oxidation of hydrogen peroxide nium / peroxidase. These oxidations are detected by fluorescence, chemiluminescence and nitration of phenols (screened by HPLC). Indirect antioxidant effects of glyptins are measured in isolated human leukocytes (PMN) by interference with an oxidase burst (activation of NADPH oxidase) induced by the forbol ester PDBu, the endotoxins LPS and zymosan A and the chemotactic peptide fMLP.
Direct vasodilatory effects of glyptins are measured by the isometric tension technique in isolated aortic ring segments. Indirect antioxidant effects of linagliptin are also tested in a rat model of nitroglycerin-induced tolerance and treatment with linagliptin (3-10 mg / kg / d by special diet for 7 days) by determining endothelial function (acetylcholine-dependent relaxation of segments of pre-constricted phenylephrine aortic vessels), smooth muscle function (nitroglycerin-dependent relaxation) by isometric tension records. In addition, the formation of reactive oxygen and nitrogen species (RONS) is determined in cardiac mitochondria and oxidative explosion triggered by LPS or PDBu in whole blood. Also, the anti-inflammatory potential is tested in an experimental model of LPS-induced septic shock (10 mg / kg i.p. for 24 hours) in Wistar rats. The effects of sepsis and linagliptin co-therapy (3-10 mg / kg / day by special diet for 7 days) are assessed by isometric, vascular, cardiac, and blood RONS formation and protein expression records by Western blotting. Results: (See Figs. 1-6) Direct antioxidant properties:
All glyptins show only direct, marginal antioxidant capacity. Mild (but significant) suppression of superoxide formation is seen in vildagliptin and linagliptin in response to nitration mediated by peroxynitrite formation. Except for saxagliptin, all gliptins show significant interference with electron oxidations 1 by the hydrogen peroxide / peroxidase system with linagliptin being the most potent compound. Indirect antioxidant properties in isolated human neutrophils:
Linagliptin shows the best inhibition of oxidative explosion in human leukocytes isolated in response to the activation of NADPH oxidase by LPS and zymosan A. Using L-012-enhanced chemiluminescence, LPS (0.5, 5 and 50 μg / mL) increases the PMN-derived RONS signal in a concentration-dependent mode and linagliptin suppresses the concentration-dependent signal.
In experiments with luminol / peroxidase, a chi-myuminuminescence enhancer, linagliptin is much more effective in suppressing the oxidative explosion triggered by LPS or zymosan A in isolated PMN than other gliptins, linagliptin is as effective as nebivolol. The effectiveness in inhibiting the formation of LPS-dependent RONs is slightly more pronounced than the suppressive effect on RONS triggered by zimozane A. All of these measurements support a superior antioxidant effect of linagliptin on isolated neutrophils compared to other gliptins.
Inhibition of adherence of activated neutrophils to endothelial cells:
When studying the adherence of human neutrophils stimulated by LPS to cultured endothelial cells (the number of adherent PMN correlates with the oxidative explosion triggered by PDBu, which can be measured by fluorescence with Amplex red / peroxidase), the linglipine suppresses leukocyte adherence to endothelial cells in the presence of LPS. Treatment of vascular dysfunction and / or oxidative stress:
Effects of treatment with oral linagliptin on vascular dysfunction and oxidative stress in nitrate tolerant rats;
Studies on isometric tension in organ baths reveal that nitroglycerin and treatment with LPS induces remarkable endothelial dysfunction and nitrate tolerance. Endothelial dysfunction caused by both factors is significantly improved by linagliptin therapy (Figs. 8A and 8B) whereas nitrate tolerance is not altered. Treatment with nitroglycerin evokes an increase in the formation of cardiac mitochondrial ROS and an oxidative explosion triggered by LPS / zimozane A in whole blood. All of these adverse effects are enhanced by treatment with linagliptin (Fig. 7). Treatment with nitroglycerin or treatment with linagliptin has no effect on the human body of animals whereas blood glucose levels are slightly increased in the nitroglycerin group that is normalized by treatment with linagliptin.
In summary, in vivo treatment with linagliptin attenuates endothelial dysfunction induced by nitroglycerin and shows a small improvement in the formation of ROS in isolated cardiac mitochondria and in the oxidative explosion in whole blood of nitrate-tolerant rats.
The effects of treatment with linagliptin on vascular dysfunction and oxidative stress in septic rats:
Very similar protective effects for linagliptin are seen in an experimental model of septic shock. Vascular function (Ach-, GTN- dependent relaxation and NONOate diethylamine) is highly compromised and almost normalized by linagliptin therapy. Production of mitochondrial and whole blood RONS (stimulators by LPS, PDBu) is dramatically increased by LPS and improved by treatment with linagliptin. Vascular oxidative stress (measured by DHE-dependent fluorescent microtopography) and vascular inflammation markers (VCAM-1, Cox-2, and NOS-2) are dramatically increased by treatment with LPS and significantly enhanced by therapy with linagliptin. Similar effects are observed for nitration of the aortic tyrosine protein and the content of malondialdehyde (both markers for oxidative stress) as well as expression of aortic NADPH oxidase subunit (Nox1 and Nox2). As proof of the concept, DPP-4 activity and GLP-1 levels are detected in the respective animals and demonstrate potent DPP-4 and an approximately 10-fold increase in plasma GLP-1 levels. Vasodilatory effects of glyptins:
The recording of the isometric tension reveals that several glyptins show direct vasodilatory effects in the concentration range of 10-100 μM. Linagliptin is the most potent compound directly followed by alogliptin and vildagliptin, whereas sitagliptin and saxagliptin are no more effective in inducing vasodilation than solvent control alone (DMSO) (see Figs. 9A and 9B).
These observations support the pleiotropic anti-oxidant and anti-inflammatory properties of linagliptin, which are not (or to a lesser extent) shared by other gliptins. In addition, linagliptin reduces leukocyte adherence to endothelial cells due to the presence of LPS and improves endothelial dysfunction and oxidative stress induced by nitroglycerin and inflammation. This can contribute to improved endothelial function and support for the cardioprotective action of linagliptin. Thus, there is evidence that linagliptin confers antioxidant effects that beneficially influence cardiovascular diseases, which are secondary to diabetic complications with high levels of morbidity and mortality. Treatment of diabetic nephropathy and albuminuria
Endothelial injury is characteristic of type 2 diabetes and contributes to the development of end-stage kidney disease. Thus, the endothelial vascular activity of NO synthase (eNOS) is altered in T2D and genetic abnormalities in the respective gene (NOS3) are associated with the development of advanced diabetic nephropathy (DN) in patients with type 1 and type 2 diabetes. The use of STZ at a low dose to induce T2D in this genetic phenotype (eNOD - / -) was recently reported (Brosius et al., JASN 2009) as a valid experimental model for DN.
ENOS - / - mice of eight weeks of age were transformed into diabetics with intraperitoneal injections of streptozotoxin (100 mg / kg per day for two consecutive days). The development of diabetes (defined by blood glucose> 250 mg / dL) was observed one week after the injection of streptozotocin. No insulin was administered, as this could prevent the development of diabetic nephropathy. Mice are treated for 4 weeks with: 1) Enos KO control mice, non-diabetic, placebo (natrosol) (n = 14) 2) eNOS ko mice, for placebo (Sham), diabetics, placebo (natrosol) (n = 7) 3) eNOS ko diabetic mice treated with telmisartan (po 1 mg / kg) (n = 17) 4) eNOS ko diabetic mice treated with linagliptin inhibitor (po 3 mg / kg) (n = 14) 5) eNOS mice ko diabetics treated with Telmisartan (po 1 mg / kg) + Linagliptin (n = 12)
Renal function (s-creatinine, albuminuria) and blood glucose levels are detected.
No significant differences in blood sugar were detected after treatment with linagliptin, telmisartan or the combination versus placebo in animals treated with STZ (see Fig. 10).
Although no effect on blood glucose was detected, the albumin / creatinine ratio is significantly reduced in the group receiving linagliptin + telmisartan (middle bar, No. 5 in Fig. 11). The respective mono-treatment also reduces the albumin / creatinine ratio, without, however, reaching significance. Also, the albumin / creatinine ratio of non-diabetic animals vs. diabetics is significantly reduced (see Fig. 11). These effects support the use of linagliptin and telmisartan in renal protection and in the treatment and / or prevention of diabetic nephropathy and albuminuria. The combination of linagliptin and telmisartan offers a new therapeutic approach to patients with or at risk of diabetic nephropathy and albuminuria. Treatment of congestive heart failure and cardiac hypertrophy:
The hypothesis was formulated here that the supply of glycosis / energy is particularly important in heart failure, which is characterized by cardiac hypertrophy. Inadequate energy supply is considered to be one of the most important stages of compensated to decompensated left ventricular hypertrophy resulting in heart failure. A classic model of hypertension-induced left ventricular hypertrophy that results in long-term left ventricular failure and pathological remodeling is the model (model 2K1C) 2 kidney renovascular hypertension - 1 clip.
The animals are treated for 3 months according to the following regimen: 1. Rats 2K1C, Telmisartan in drinking water (10 mg / kg KG) (n = 14) 2. Rats 2K1C, Linagliptin (BI1356) in the feed (89 ppm, corresponding to 3-10 mg / kg of oral gavage) (n = 15). 3. Rats 2K1C, Telmisartan (10 mg / kg) + Linagliptin (BI1356) in the ration (89 ppm) (n = 15) 4. Rats 2K1C, placebo (n = 17) 5. SHAM rats, placebo (n = 11) .
Non-invasive systolic blood pressure is measured in all groups for time points (1. before treatment; 2. after 1 week; 3. after four weeks; 4. after 6 weeks; 5. after 12 weeks, and 6. after 6 weeks of treatment with the respective compounds.
Before treatment, only the animals treated with were significantly different from others in the other groups. After 1 week of treatment until the end of the study, telmisartan and the combination of telmisartan and linagliptin were always significant vs. vehicle treated animals. The combination of telmisartan and linagliptin reaches the level of placebo animals (sham) treated with placebo and shows additional effects to mono-treatment with telmisartan (see Fig. 12). These effects support the use of linagliptin and telmisartan in the treatment and / or prevention of cardiac hypertrophy and / or congestive heart failure. The combination of linagliptin and telmisartan offers a new therapeutic approach for patients with or at risk for cardiac hypertrophy and / or congestive heart failure. Treatment of uremic cardiomyopathy
Uremic cardiomyopathy substantially contributes to the morbidity and mortality of patients with chronic kidney disease, which, in turn, is a frequent complication in type 2 diabetes. Glucagon-like peptide 1 (GLP-1) can improve cardiac function and LPG -1 is mainly degraded by dipeptidyl peptidase-4 (DPP-4). Linagliptin is the only DDP-4 inhibitor that can be used clinically (for example, in patients with type 2 diabetes and diabetic nephropathy) in all stages of renal failure without dose adjustment.
Linagliptin was investigated in a rat model of chronic renal failure (5; 6 for nephrectomy [5 / 6N);
Eight weeks after 5 / 6N or "sham" surgery, the rats are treated with 3.3 mg / kg of linagliptin or vehicle for 4 days, and subsequently, plasma is sampled for 72 hours to quantify DPP- activity. 4 and GLP-1 levels. At the end of the study, cardiac tissue is harvested for mRNA analysis. 5 / 6N causes a significant decrease (p <0.001 (in GFR measured by creatinine clearance ("sham": 2510 ± 210 mU24 h; 5 / 6N: 1665 ± 104.3 mU24 h) and increased levels of cystatin C (" sham ": 700 ± 35.7 ng / mL; 5 / 6N: 1434 ± 77.6 ng / mL). DPP-4 activity is significantly reduced at all time points with no difference between" sham "or animals 5 / 6N In contrast, the levels of active GLP increased significantly in animals 5 / 6N, as measured by the maximum plasma concentration (CmaX; 5 / 6N: 6.36 ± 2.58 pg / mL vs sham: 3, 91 ± 1.86 pg / mL; p <0.001) and AUC (0-72h) (5 / 6N: 201 pg * h / mL vs. "sham": 114 pg * h / mL; p <0.001). mRNA levels of cardiac fibrosis markers (pro-fibrotic factors) such as TGF-β, matrix metalloproteinase 1 tissue inhibitor (TIMP-1) and collagen 1a1 and 3a1 as well as markers of left ventricular dysfunction, cerebral natriuretic peptide ( NP) are all significantly increased by 5 / 6N vs. "sham" animals and are consequently reduced s or even normalized by treatment with linagliptin (all p <0.05, see Fig. 13).
Linagliptin increases GLP-1 AUC by about 2-fold in a mouse model with renal failure, and decreases gene expression of BNP, a marker of left ventricular dysfunction, as well as markers of cardiac fibrosis (TGF-β, TIMP -1, Col 1α1 and Col 3a1) in the hearts of uremic rats. These effects support the use of linagliptin in the treatment and / or prevention of uremic cardiomyopathy. Linagliptin offers a new therapeutic approach for patients with uremic cardiomyopathy. Effect on infarction size and cardiac function after myocardial ischemia / reperfusion:
The purpose of this study is to assess the cardiac effects (particularly on myocardial ischemia / reperfusion, cardiac function, or infarct size) of a xanthine-based DPP-4 inhibitor of this invention, such as, for example, in conditions involving factor-1 alpha derived from stromal cells (SDF-1a).
Male Wistar rats were divided into 3 groups: "sham", is-chia / reperfusion (l / R), and l / R + DPP-4 inhibitor of this invention; n = 10-12 per group. The DPP-4 inhibitor is given once daily starting two days before the L / R. The anterior descending coronary artery is ligated for 30 minutes. Echocardiography is performed after 5 days and cardiac characterization after 7 days. The DPP-4 inhibitor significantly reduces the size of the absolute infarction (-27.8%; p <0.05), the proportion of infarcted tissue in relation to the total risk area (-18.5 "; p < 0.05) and the extent of myocardial fibrosis (-31.6%; p <0.05). The DPP-4 inhibitor significantly increases the accumulation of stem / progenitor cells as characterized by expression of CD34, CXCR4 and C-kit and C-kit and cardiac immunoreactivity for active SDF-1a in the infarcted myocardium The left ventricular ejection fraction is similar in all Ml groups after 7 days, however, the inhibition of DPP-4 reduces the size of infarction, reduces fibrotic remodeling and increases the density of stem cells in infarcted areas by blocking the degradation of SDF-1a.
An xanthine-based DPP-4 inhibitor of this invention is capable of reducing the size of the infarction after myocardial infarction. Mechanisms of action may include reduced degradation of SDF-1α with subsequently increased recruitment of CXCR-4 + stem cells and / or pathways dependent on incretin receptors.
These data reinforce the usefulness of a xanthine-based DPP-4 inhibitor of this invention to increase stem cells, increase tissue repair, activate myocardial regeneration, reduce the size of the infarction, reduce fibrotic remodeling and / or increase the density of stem cells in infarcted cardiac areas in the treatment or prevention of myocardial ischemia / reperfusion and / or in cardioprotection.
Based on the size of the infarction, it is a prognosticator of future events (including mortality), it is postulated that a xanthine-based DPP-4 inhibitor of this invention may also be useful to increase cardiac (systolic) function, cardiac contractility and / or mortality after myocardial ischemia / reperfusion. Effect of linagliptin on infarction size and cardiac function after myocardial ischemia / reperfusion
Materials and methods: Male Wistar rats are divided into three groups: "sham", l / R and l / R + linagliptin (n = 1-18 per group). Linagliptin is given once daily (3 mg / kg), starting 30 days before L / R. l / R is induced by ligation of the left anterior descending coronary artery for 30 minutes. Echocardiography is performed after 58 days and cardiac catheterization after 60 days.
Linagliptin significantly reduces the proportion of the infarcted tissue relative to the total area at risk (-21%; p <0.001) as well as the size of the absolute infarction (-18%; p <0.05) in this ischemic injury model / reperfusion (l / R). In addition, glucagon-like peptide-1 levels (GLP-1) are increased 18-fold (p <0.0001) and DPP-4 activity is reduced by 78% (p <0.0001). The diastolic and systolic pressure of the left ventricular left extremity and all echocardiography parameters are similar between the groups, with a significant improvement in the isovolumetric contractility indexes (dP / dTmin) from -4771 ± 79 mmHg / s to - 4957 ± 73 mmHg / s or improved maximum rate of decline in left ventricular pressure. These data also support a cardioprotective function of linagliptin in the condition of acute myocardial infarction. Treatment of ARB-resistant diabetic nephropathy:
The need for an improved treatment for diabetic nephropathy is greater in patients who do not respond to angiotensin receptor blockers (ARBs). This study investigates the effect of linagliptin, alone and in combination with telmisartan ARB, on the progress of diabetic nephropathy in eNOS knockout mice, a pathology of similarity close to the new model.
Twenty-six male mice, C57BL / 6J knockout e-NOS, are divided into 4 groups after receiving a high intraperitoneal dose of streptozotocin: telmisartan (1 mg / kg), linagliptin (3 mg / kg), lina-glyiptine + telmisartan (3 + 1 mg / kg), and vehicle. Fourteen mice are used as non-diabetic controls. After 12 weeks, urine and blood are obtained and blood pressure is measured. Glucose concentrations are increased and similar in all diabetic groups. Telmisartan alone lowers blood pressure modestly by 5.9 mmHg vs. diabetic controls (111.2 + 2.3 mmHg vs. 117.1 ± 2.2 mmHg; mean ± SEM; n = 4 each; p = 0.071) and none of the other treatments reached significance. Combined treatment significantly reduces albuminuria (eg, urinary albumin excretion for 24 hours and / or the albumin / creatinine ratio) compared to diabetic controls (71.7 ± 15.3 μg / 24 h vs. 170, 8 ± 34.2 pg / 24 h; n = 12-13; p = 0.017), whereas the effects of simple or telmisartan treatment (97.8 ± 26.4 μg / 24 h; n = 14) or linagliptin (120.8 ± 37.7 μg / 24 h; n = 11) are not statistically significant (see Fig. 14). Linagliptin, alone or in combination, leads to levels of osteopon-
Plasma concentrations of TNF-a are significantly lower in all treatment groups than with the vehicle. Levels of lipocalin associated with neutrophil gelatinase (NGAL) are significantly increased after treatment with telmisartan with untreated diabetic mice, where this effect is prevented by combined treatment with linagliptin.
In addition, linagliptin, alone and in combination with tel-misartan, leads to an average kidney glomerulosclerosis by histological score compared to diabetic controls (2.1 +/- 0.0 vs. 2.4 +/- 0.0; 10 p <0.05), while the reduction obtained by telmisartan alone is not significantly different. In conclusion, linagliptin significantly reduces urinary albumin excretion in knockout, eNOS, diabetic mice, which are refractory to ARB (for example, in a blood pressure independent mode). These effects may support the use of linagliptin 15 in renoprotection and in the treatment and / or prevention of ARB-resistant diabetic nephropathy. Linagliptin may offer a new therapeutic approach for patients resistant to ARB treatment. Delayed onset of diabetes and conservation of beta cell function in non-obesity type 1 diabetes:
Although the migration of pancreatic T cells and the production of cytokines are considered to be executors for the onset of insulinitis, the exact mechanism and effects and effects on pancreatic cell assembly are not yet fully understood. In an attempt to assess the effect of linagliptin on pancreatic inflammation and beta cell mass, the progression of diabetes in non-obese mice (NOD) was examined for a 60-day experimental period coupled with the terminal stereological evaluation of cellular pancreatic changes.
Sixty female NOD mice (10 weeks old) were included in the study and fed either a normal feed diet or a diet containing linagliptin (0.083 g of linagliptin / kg of feed; corresponding to 3-10 mg / kg, po) through study period. Two-week plasma samples are taken to determine the onset of diabetes (BG> 1 mmols / L). At the end, the pancreas is removed and a final blood sample is obtained to assess the levels of active GLP-1.
At the end of the study period, the incidence of diabetes is significantly decreased in mice treated with linagliptin (9 out of 30 mice with the control group (18 out of 30 mice, p = 0.021). The subsequent stereological assessment of cell mass beta (identified by insulin immunoreactivity) demonstrates a significantly higher beta cell mass (veh 0.18 + 0.03 mg; blade 0.48 + 0.09 mg, p <0.01) and total islet mass (veh ± 0.04 mg; blade 0.70 ± 0.09 mg, p <0.01) in mice treated with linagliptin. There is a tendency for linagliptin to reduce lymphocytes that infiltrate the Peri-islets (1.06 ± 0.14; line 0.79 ± 0.12 mg, p + 0.17) As expected, active plasma GLP-1 are greater in termination of mice treated with linagliptin.
In summary, the data demonstrate that linagliptin is capable of delaying the onset of diabetes in a type 1 diabetic model (NOD mouse). The pronounced sparse effects of beta cells, which can be seen in this animal model, indicate that such an inhibition that such DPP-4 inhibition protects beta cells by increasing the levels of active GLP-1, but may also have anti-inflammatory actions. direct or indirect inflammatory diseases. These effects may support the use of linagliptin in the treatment and / or prevention of type 1 diabetes or latent autoimmune diabetes in adults (LADA). Linagliptin may offer a new therapeutic approach for patients with or at risk for type 1 diabetes or LADA. Effect of linagliptin on total body weight, liver fat and intramyocellular fat
In another study, the effectiveness of chronic treatment with linagliptin on body weight, total body fat, intramiocellular fat, and liver fat in a non-diabetic model of diet-induced obesity (DIO) compared to appetite suppressing subtramine was investigated:
The rats are fed a high-fat diet for 3 months and received either vehicle, linagliptin (10 mg / kg), or subtramine (5 mg / LG) for an additional 6 weeks, while continuing with the high-fat diet. Magnetic resonance spectroscopy (MRS) analysis of total body fat, muscle fat, and liver fat is performed before treatment and at the end of the study.
Subtramine causes a significant reduction in body weight (- 12%) vs. control, whereas linagliptin has no significant effect (-3%). Total body fat decreased significantly by subtramine (-12%), whereas animals treated with linagliptin show no significant reduction (-5% 0. However, both linagliptin and subtramine 10 result in a potent reduction in intramiocellular fat ( -24% and 34%, respectively). In addition, treatment with linagliptin results in a marked increase in liver fat (-39%), while the effect of si-butramine (-30%) does not reach significance (see Table below.) Thus, linagliptin is of natural weight, but improves accumulation of intramiocellular and liver lipid 15. A reduction in steatosis, inflammation and fibrosis in the liver, measured by histological score, is also measured by histological score is also observed for treatment of linagliptin Table: Effect of linagliptin on fat from fat weight, fat from liver and intramiocellular fat

In conclusion, treatment with linagliptin causes a potent reduction in intramiocellular lipids and liver fat, which are both independent of weight loss. Treatment with linagliptin provides an additional benefit to patients with diabetes who are still affected by fatty liver (for example, NAFLD). The effects of sibutramine on muscle fat and liver fat are mainly attributed to the known weight reduction induced by this compound. Linagliptin has similar efficacy to glimepiride, but improves cardiovascular safety for 2 years in patients with type 2 diabetes uncontrollably controlled on metformin:
In a 2-year double-blind experiment, the long-term efficacy and safety of adding linagliptin or glimepiride to the ongoing metformin to treat type 2 diabetes (T2DM) is investigated. Patients with T2DM on stable metformin (> 15000 mg / day) for> 10 weeks are randomized to linagliptin 5 mg / day (N = 764) or glimepiride 1-4 mg / day (N = 755) for 2 years. The analyzes for verification of efficacy are based on the change in HbA 1c from the baseline in the total set of analysis (FAZ) and population per protocol (PP). Safety assessments include pre-specified, prospective, and adjudicated capture of cardiovascular events (CV) (CV death, non-fatal myocardial infarction or stroke, unstable angina with hospitalization). Baseline characteristics are well balanced in the 2 groups (bA1c 7.7% for both). In the PP population, the adjusted mean HbA1c changes (± SE) from baseline are -0.4% (± 0.4%) for linagliptin 5 mg / day vs. -0.5% (± 0.04%) for glimepiride (average dose of 3 mg / day). The average difference between the groups is 0.17% (95% Cl, 0.08-0.27%; p = 0.0001 for non-inferiority). Similar results are seen in the FAS population. Far fewer patients suffered from drug-related hypoglycemia, defined by the investigator, with linagliptin than with glimepiride (7.5% vs. 36.1%; p <0.0001). Body weight is decreased with linagliptin and increased with glimepiride (- 1.4 kg vs. 1.3 kg; adjusted mean difference, -2.7 kg; p <0.0001). CV events occurred in 13 (1.7%) of patients on linagliptin vs. 26 (3.4%) of glimepiride patients, showing a significant 50% reduction in the relative risk for the combined CV end point (RR, 0.50; 95% Cl, 0.26-0.96; p = 0.04). In conclusion, when added to metformin monotherapy, linagliptin provides reductions in HbA1c similar to glimepiride, but with less hypoglycemia, relative weight loss, and significantly less adjudicated CV events. Cardiovascular Risk with Linagliptin in Patients with Type 2 Diabetes: A Pre-specified, Prospective and Awarded Meta-Analysis from a Large Phase III program:
The cardiovascular benefit (CV) of glucose reduction, particularly if very intensive in type 2 diabetes mellitus (T2DM) is currently debated. Some modalities were even reported, unexpectedly as being associated with the worst CV results.
Linagliptin is the first once-a-day DPP-4 inhibitor available as a dose without the need for dose adjustment for declining kidney function. Linagliptin achieves glycemic control without weight gain or increased hypoglycemic risk that can translate into CV benefits.
To investigate the CN profile of the DPP-4 inhibitor linagliptin, a pre-specified meta-analysis of all CV events from 8 controlled, double-blind, randomized phase III trials (> 12 weeks) was conducted. CV events are prospectively awarded by a specialized, independent, blind committee. The primary end point of this analysis is a composition of CV death, non-fatal stroke, non-fatal myocardial infarction (Ml), and hospitalization for UAP unstable angina pectoris. Other secondary and tertiary CV end points are also assessed, including adverse CV events, major (MACE), custom- FDA Of the 5,239 patients included (baseline HbAic 8.0%) 3,319 received linagliptin once daily (5 mg: 3,159 , 10 mg: 160) and 1,920 comparators (placebo: 977, glimepiride; 781, voglibose: 162). Cumulative exposure (person in years) is 2,060 for linagliptin and 1,372 for comparators. In all, major CV events awarded occurred in 11 patients (0.3%) who were receiving linagliptin and 23 (1.2%) who were receiving comparator. The risk ratio for the main end point is significantly lower for linagliptin vs. comparator and risk ratios are similar or significantly lower with linagliptin vs. compared for all other CV end points (TABLE).
This is the first pre-specified, prospective, and independently awarded meta-analysis of a DPP-4 inhibitor in a large Phase III program. Although a meta-analysis, with distinct limitations, the data support a potential reduction in CV events with linagliptin. TABLE:
* Significant lower risk ratio (Cl 95% higher than 1.0; p <0.05). Treatment of patients with type 2 diabetes mellitus with high cardio-vascular risk
The long-term impact on cardiovascular morbidity and mortality and relevant efficacy parameters (eg, HbA1c, fasting plasma glucose, treatment sustainability) of treatment with linagliptin in a relevant population of patients with type 2 diabetes mellitus is investigated as follows :
The type 2 diabetes patient with insufficient glycemic control (naive or currently treated (monotherapy or dual therapy) with, for example, metformin and / or alpha-glucosidase inhibitor (for example, having 6.5-8.5 % HbA1c), or currently treated (monotherapy or dual therapy) with, for example, a sulfoniurea or glinide, with or without metformin or an alpha-glucosidase inhibitor (for example, having 7.5-8.5% HbA1c)) and high risk of cardiovascular events, for example, defined as one or more indicated risk factors A), B), C) and D), are treated for a long period (for example, for> / = 2 years, 4.5 years or 1-6 years) with linagliptin (optionally in combination with one or more active substances, for example, aunts like those described here) and compared to patients who have been treated with other anti-diabetic drugs (for example, a sulfonylurea, such as glimepiride) or with placebo. Evidence of therapeutic success compared to patients who have been treated with single or multiple complications (eg, cardiovascular or cerebrovascular events such as cardiovascular death, myocardial infarction, stroke, or hospitalization (eg, acute coronary syndrome, leg amputation) , urgent revascularization procedures or for unstable angina pectoris)), or, preferably, the longest time taken for the first occurrence of such complications, for example, time for the first occurrence of any of the following endpoint components primary compound: cardiovascular death, non-fatal myocardial infarction, non-fatal stroke and hospitalization for unstable angina pectoris.
Additional therapeutic success can be found in a greater proportion of patients in the study treatment at the end of the study who maintain glycemic control (for example, HbA1c </ + 7%) without the need for rescue medication and without weight gain (for example ,> / = 2%). Additional therapeutic success can be found in a greater proportion of patients in the study treatment at the end of the study who maintain glycemic control (eg, HbA1c </ + 7%) without the need for rescue medication and without moderate / severe hypoglycemic episodes and no gain weight (for example,> / = 2%).
Another therapeutic success can be found, for example, in the CV superiority of treatment with linagliptin vs. treatment with glimepyride (each optionally as mono-therapy or as therapy added to metformin or an alpha-glucosidase inhibitor) with risk reduction of preferably about 20%, for example.
Risk factors A), B), C) and D) for cardiovascular events: A) Previous vascular disease (for example,> 1 = 6 weeks): - myocardial infarction (for example,> / = 50% narrowing of the luminal diameter of the left coronary artery or in at least two main coronary arteries on the angiogram), - percutaneous coronary intervention (eg> / = 6 weeks), - myocardial revascularization surgery (eg> / = 4 years or with recurrent angina following surgery), - ischemic or hemorrhagic stroke (for example,> 1 = 3 months), - peripheral arterial occlusive disease (for example, prior revascularization surgery of the limbs or percutaneous transluminal angioplasty; previous amputation of the limb or of the foot for circulatory failure, stenosis of the significant vessel detected by angiography or ultrasound (> 50%) of the main arteries of the limbs (common iliac artery, internal iliac artery, external iliac artery, femoral artery, politea artery l) intermittent claudication, with an ankle blood pressure ratio: arm <0.90 on at least one side), B) Injury to the vascular related target organ (eg, age 40-85 years); - impaired renal function (for example, moderately impaired renal function as defined by the MDRD formula, with eG-FRF of 30-59 mL / min / 1.73 m2), - micro or macroalbuminuria (for example, microalbuminuria, or dot ratio random urinary albuminxreatinin> / = 30 μg / mg), - retinopathy (eg, proliferative retinopathy, or retinal neovascularization or retinal laser coagulation therapy), C) Elderly (eg, age> / = 70 years) , D) At least two of the following cardiovascular risk factors (for example, age 40-85 years): - advanced type 2 diabetes mellitus (for example,> 10 years in duration), - hypertension (for example, systolic blood pressure > 140 mmGHg or at least one blood pressure lowering treatment), - current daily cigarette smoker, - dyslipidemia (atherogenic) or high blood levels of LDL cholesterol (eg LDL cholesterol> / = 135 mg / dL ) or in at least one treatment for lipid abnormality, - obesi (visceral and / or abdominal) (for example, body mass index> / = 45 kg / m2), - age> / = 40 and </ = 80 years.
Beneficial effects (eg, improvement) of cognitive function (eg, cognitive decline, changes in psychomotor speed, psychological well-being), β cell function (eg, insulin secretion rate derived from a 3 hour meal tolerance test, long-term β cell function), renal function parameters, daytime glucose pattern (eg ambulatory glucose profile, glycemic variability, oxidation biomarkers, inflammation of endothelial function, cognition and CV morbidity / mortality), silent Ml (for example, ECG parameters, CV prophylactic properties), LADA (for example, use of rescue therapy or disease progression in LADA) and / or glucose control durability of β cell antibody status (eg GAD) from linagliptin treatment.

权利要求:
Claims (8)
[0001]
1. Use of linagliptin, characterized by the fact that it is to prepare a pharmaceutical composition to treat diabetic nephropathy in a patient who does not respond adequately to therapy with an angiotensin receptor blocker (ARB), in which the pharmaceutical composition contains a therapeutically effective amount of linagliptin.
[0002]
2. Use according to claim 1, characterized by the fact that the patient has type 2 diabetes.
[0003]
Use according to claim 1 or 2, characterized in that the therapeutically effective amount of linagliptin can be used in combination with an angiotensin receptor blocker (ARB).
[0004]
Use according to any one of claims 1 to 3, characterized in that the angiotensin receptor blocker (ARB) is telmisartan.
[0005]
5. Use of linagliptin, characterized by the fact that it is to prepare a pharmaceutical composition to treat diabetic nephropathy in a patient who does not respond adequately to therapy with an angiotensin receptor blocker (ARB), in which the pharmaceutical composition contains a therapeutically effective amount of linagliptin and is packaged in the form of an article containing instructions that such a pharmaceutical composition can be used in combination.
[0006]
6. Use according to claim 5, characterized by the fact that the patient has type 2 diabetes.
[0007]
Use according to claim 5 or 6, characterized in that the pharmaceutical composition can be used in combination with an angiotensin receptor blocker (ARB).
[0008]
Use according to any one of claims 5 to 7, characterized in that the angiotensin receptor blocker (ARB) is telmisartan.

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法律状态:
2018-01-23| B07D| Technical examination (opinion) related to article 229 of industrial property law [chapter 7.4 patent gazette]|

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2018-04-03| B06F| Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]|

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2019-07-02| B07E| Notice of approval relating to section 229 industrial property law [chapter 7.5 patent gazette]|Free format text: NOTIFICACAO DE ANUENCIA RELACIONADA COM O ART 229 DA LPI |

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2019-09-10| B06U| Preliminary requirement: requests with searches performed by other patent offices: procedure suspended [chapter 6.21 patent gazette]|

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2019-09-17| B06I| Publication of requirement cancelled [chapter 6.9 patent gazette]|Free format text: ANULADA A PUBLICACAO CODIGO 6.21 NA RPI NO 2540 DE 10/09/2019 POR TER SIDO INDEVIDA. |

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2020-09-01| B06A| Notification to applicant to reply to the report for non-patentability or inadequacy of the application [chapter 6.1 patent gazette]|

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2020-12-08| B09A| Decision: intention to grant [chapter 9.1 patent gazette]|

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2021-02-09| B16A| Patent or certificate of addition of invention granted|Free format text: PRAZO DE VALIDADE: 20 (VINTE) ANOS CONTADOS A PARTIR DE 15/11/2011, OBSERVADAS AS CONDICOES LEGAIS. |

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2021-08-17| B17A| Notification of administrative nullity (patentee has 60 days time to reply to this notification)|Free format text: REQUERENTE DA NULIDADE: LIBBS FARMACEUTICA LTDA - 870210071842 - 6/08/2021 |

优先权:

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申请号 | 申请日 | 专利标题

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EP10191261|2010-11-15|

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EP10191261.6|2010-11-15|

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US41554510P| true| 2010-11-19|2010-11-19|

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US61/415,545|2010-11-19|

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US42140010P| true| 2010-12-09|2010-12-09|

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US61/421,400|2010-12-09|

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EP11168317|2011-05-31|

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US201161492391P| true| 2011-06-02|2011-06-02|

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US61/492,391|2011-06-02|

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EP11170992.9|2011-06-22|

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EP11170992|2011-06-22|

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PCT/EP2011/070156|WO2012065993A1|2010-11-15|2011-11-15|Vasoprotective and cardioprotective antidiabetic therapy|

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